LOCUS       DARBFD010000001        95142 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000001 DARBFD010000000
VERSION     DARBFD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 95142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.001"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(19..729)
                     /locus_tag="SCK08_000001"
     CDS             complement(19..729)
                     /locus_tag="SCK08_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEG1199924.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="SCK08_000002"
     CDS             complement(726..2198)
                     /locus_tag="SCK08_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1199925.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="SCK08_000003"
     CDS             2336..2809
                     /locus_tag="SCK08_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1199926.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="SCK08_000004"
     CDS             complement(2962..3513)
                     /locus_tag="SCK08_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1199927.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="SCK08_000005"
     CDS             3618..4148
                     /locus_tag="SCK08_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEG1199928.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="SCK08_000006"
     CDS             complement(4631..5074)
                     /locus_tag="SCK08_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1199929.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="SCK08_000007"
     CDS             complement(5090..6250)
                     /locus_tag="SCK08_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1199930.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="SCK08_000008"
     CDS             6757..8457
                     /locus_tag="SCK08_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1199931.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="SCK08_000009"
     CDS             complement(8559..9392)
                     /locus_tag="SCK08_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEG1199932.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="SCK08_000010"
     CDS             complement(9696..11861)
                     /locus_tag="SCK08_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1199933.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="SCK08_000011"
     CDS             12121..14415
                     /locus_tag="SCK08_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1199934.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="SCK08_000012"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="SCK08_000012"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1199935.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            15066..16073
                     /locus_tag="SCK08_000013"
     CDS             15066..16073
                     /locus_tag="SCK08_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1199936.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="SCK08_000014"
     CDS             16095..17114
                     /locus_tag="SCK08_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1199937.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="SCK08_000015"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="SCK08_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1199938.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18872..20293)
                     /locus_tag="SCK08_000016"
     CDS             complement(18872..20293)
                     /locus_tag="SCK08_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1199939.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20536..22413)
                     /gene="tdc"
                     /locus_tag="SCK08_000017"
     CDS             complement(20536..22413)
                     /gene="tdc"
                     /locus_tag="SCK08_000017"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1199940.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22704..23960)
                     /locus_tag="SCK08_000018"
     CDS             complement(22704..23960)
                     /locus_tag="SCK08_000018"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEG1199941.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24072..24277)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24540..25841
                     /locus_tag="SCK08_000019"
     CDS             24540..25841
                     /locus_tag="SCK08_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1199942.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25910..26509)
                     /locus_tag="SCK08_000020"
     CDS             complement(25910..26509)
                     /locus_tag="SCK08_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1199943.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26763..27644
                     /locus_tag="SCK08_000021"
     CDS             26763..27644
                     /locus_tag="SCK08_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1199944.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27657..29267
                     /locus_tag="SCK08_000022"
     CDS             27657..29267
                     /locus_tag="SCK08_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEG1199945.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29344..30003)
                     /locus_tag="SCK08_000023"
     CDS             complement(29344..30003)
                     /locus_tag="SCK08_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1199946.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30286..30951)
                     /locus_tag="SCK08_000024"
     CDS             complement(30286..30951)
                     /locus_tag="SCK08_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEG1199947.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(31098..31703)
                     /gene="dhaL"
                     /locus_tag="SCK08_000025"
     CDS             complement(31098..31703)
                     /gene="dhaL"
                     /locus_tag="SCK08_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEG1199948.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(31731..32741)
                     /gene="dhaK"
                     /locus_tag="SCK08_000026"
     CDS             complement(31731..32741)
                     /gene="dhaK"
                     /locus_tag="SCK08_000026"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEG1199949.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(32716..33162)
                     /gene="dhaM"
                     /locus_tag="SCK08_000027"
     CDS             complement(32716..33162)
                     /gene="dhaM"
                     /locus_tag="SCK08_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEG1199950.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            33265..33585
                     /locus_tag="SCK08_000028"
     CDS             33265..33585
                     /locus_tag="SCK08_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEG1199951.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(33697..34566)
                     /locus_tag="SCK08_000029"
     CDS             complement(33697..34566)
                     /locus_tag="SCK08_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1199952.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(34662..35965)
                     /locus_tag="SCK08_000030"
                     /pseudo
     CDS             complement(34662..35965)
                     /locus_tag="SCK08_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(35962..36471)
                     /locus_tag="SCK08_000031"
     CDS             complement(35962..36471)
                     /locus_tag="SCK08_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1199953.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(36646..38781)
                     /locus_tag="SCK08_000032"
     CDS             complement(36646..38781)
                     /locus_tag="SCK08_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1199954.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(38864..40519)
                     /locus_tag="SCK08_000033"
     CDS             complement(38864..40519)
                     /locus_tag="SCK08_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1199955.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(40521..40835)
                     /locus_tag="SCK08_000034"
     CDS             complement(40521..40835)
                     /locus_tag="SCK08_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1199956.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            41005..41265
                     /locus_tag="SCK08_000035"
     CDS             41005..41265
                     /locus_tag="SCK08_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEG1199957.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            41279..41593
                     /locus_tag="SCK08_000036"
     CDS             41279..41593
                     /locus_tag="SCK08_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEG1199958.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(42323..42877)
                     /locus_tag="SCK08_000037"
     CDS             complement(42323..42877)
                     /locus_tag="SCK08_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEG1199959.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(43003..44931)
                     /locus_tag="SCK08_000038"
     CDS             complement(43003..44931)
                     /locus_tag="SCK08_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1199960.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(45237..45953)
                     /locus_tag="SCK08_000039"
     CDS             complement(45237..45953)
                     /locus_tag="SCK08_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1199961.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(45954..47777)
                     /gene="glpO"
                     /locus_tag="SCK08_000040"
     CDS             complement(45954..47777)
                     /gene="glpO"
                     /locus_tag="SCK08_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEG1199962.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(47791..49287)
                     /gene="glpK"
                     /locus_tag="SCK08_000041"
     CDS             complement(47791..49287)
                     /gene="glpK"
                     /locus_tag="SCK08_000041"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEG1199963.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(49423..50883)
                     /locus_tag="SCK08_000042"
     CDS             complement(49423..50883)
                     /locus_tag="SCK08_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1199964.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(50873..52168)
                     /locus_tag="SCK08_000043"
     CDS             complement(50873..52168)
                     /locus_tag="SCK08_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1199965.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(52500..53177)
                     /locus_tag="SCK08_000044"
     CDS             complement(52500..53177)
                     /locus_tag="SCK08_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEG1199966.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(53207..54298)
                     /locus_tag="SCK08_000045"
     CDS             complement(53207..54298)
                     /locus_tag="SCK08_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEG1199967.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(54399..54833)
                     /locus_tag="SCK08_000046"
     CDS             complement(54399..54833)
                     /locus_tag="SCK08_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1199968.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(55417..56874)
                     /gene="ascB"
                     /locus_tag="SCK08_000047"
     CDS             complement(55417..56874)
                     /gene="ascB"
                     /locus_tag="SCK08_000047"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1199969.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(56871..58766)
                     /locus_tag="SCK08_000048"
     CDS             complement(56871..58766)
                     /locus_tag="SCK08_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1199970.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(58824..60599)
                     /locus_tag="SCK08_000049"
     CDS             complement(58824..60599)
                     /locus_tag="SCK08_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1199971.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(60649..62145)
                     /locus_tag="SCK08_000050"
     CDS             complement(60649..62145)
                     /locus_tag="SCK08_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1199972.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(62339..63961)
                     /locus_tag="SCK08_000051"
     CDS             complement(62339..63961)
                     /locus_tag="SCK08_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1199973.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(64410..65723)
                     /locus_tag="SCK08_000052"
     CDS             complement(64410..65723)
                     /locus_tag="SCK08_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1199974.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(65968..66192)
                     /locus_tag="SCK08_000053"
     CDS             complement(65968..66192)
                     /locus_tag="SCK08_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1199975.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(66212..66634)
                     /locus_tag="SCK08_000054"
     CDS             complement(66212..66634)
                     /locus_tag="SCK08_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEG1199976.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(66778..67488)
                     /locus_tag="SCK08_000055"
     CDS             complement(66778..67488)
                     /locus_tag="SCK08_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1199977.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(67506..68003)
                     /locus_tag="SCK08_000056"
     CDS             complement(67506..68003)
                     /locus_tag="SCK08_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1199978.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(68040..68492)
                     /locus_tag="SCK08_000057"
     CDS             complement(68040..68492)
                     /locus_tag="SCK08_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1199979.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(68627..69232)
                     /locus_tag="SCK08_000058"
     CDS             complement(68627..69232)
                     /locus_tag="SCK08_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1199980.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(69682..70302)
                     /locus_tag="SCK08_000059"
     CDS             complement(69682..70302)
                     /locus_tag="SCK08_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1199981.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(70304..71173)
                     /locus_tag="SCK08_000060"
     CDS             complement(70304..71173)
                     /locus_tag="SCK08_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEG1199982.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(71196..71603)
                     /locus_tag="SCK08_000061"
     CDS             complement(71196..71603)
                     /locus_tag="SCK08_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1199983.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(71622..72911)
                     /locus_tag="SCK08_000062"
     CDS             complement(71622..72911)
                     /locus_tag="SCK08_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1199984.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(72938..73240)
                     /locus_tag="SCK08_000063"
     CDS             complement(72938..73240)
                     /locus_tag="SCK08_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1199985.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(73265..75358)
                     /locus_tag="SCK08_000064"
     CDS             complement(73265..75358)
                     /locus_tag="SCK08_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1199986.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(75847..76611)
                     /locus_tag="SCK08_000065"
     CDS             complement(75847..76611)
                     /locus_tag="SCK08_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEG1199987.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(76722..77591)
                     /gene="mdcB"
                     /locus_tag="SCK08_000066"
     CDS             complement(76722..77591)
                     /gene="mdcB"
                     /locus_tag="SCK08_000066"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEG1199988.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(77616..78692)
                     /locus_tag="SCK08_000067"
     CDS             complement(77616..78692)
                     /locus_tag="SCK08_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEG1199989.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(78716..79309)
                     /locus_tag="SCK08_000068"
     CDS             complement(78716..79309)
                     /locus_tag="SCK08_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEG1199990.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(79313..80989)
                     /gene="mdcD"
                     /locus_tag="SCK08_000069"
     CDS             complement(79313..80989)
                     /gene="mdcD"
                     /locus_tag="SCK08_000069"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEG1199991.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(80979..81278)
                     /locus_tag="SCK08_000070"
     CDS             complement(80979..81278)
                     /locus_tag="SCK08_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEG1199992.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(81380..83026)
                     /gene="mdcA"
                     /locus_tag="SCK08_000071"
     CDS             complement(81380..83026)
                     /gene="mdcA"
                     /locus_tag="SCK08_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEG1199993.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(83033..83944)
                     /locus_tag="SCK08_000072"
     CDS             complement(83033..83944)
                     /locus_tag="SCK08_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1199994.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            84064..84921
                     /locus_tag="SCK08_000073"
     CDS             84064..84921
                     /locus_tag="SCK08_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1199995.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(85108..85470)
                     /locus_tag="SCK08_000074"
     CDS             complement(85108..85470)
                     /locus_tag="SCK08_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEG1199996.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            85816..87255
                     /locus_tag="SCK08_000075"
     CDS             85816..87255
                     /locus_tag="SCK08_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1199997.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(87262..89814)
                     /locus_tag="SCK08_000076"
     CDS             complement(87262..89814)
                     /locus_tag="SCK08_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEG1199998.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(89928..91259)
                     /locus_tag="SCK08_000077"
     CDS             complement(89928..91259)
                     /locus_tag="SCK08_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1199999.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(91225..92703)
                     /locus_tag="SCK08_000078"
     CDS             complement(91225..92703)
                     /locus_tag="SCK08_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1200000.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(93008..94843)
                     /gene="tdc"
                     /locus_tag="SCK08_000079"
     CDS             complement(93008..94843)
                     /gene="tdc"
                     /locus_tag="SCK08_000079"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1200001.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        27591 a        20079 c        16476 g        30996 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca cgccgcaagc ttggatcaag
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggttggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttgttttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaacagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcaga aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgagat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttacctgac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttttcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtgtca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagatc
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtac ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag acgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt tttatttttc ttctaaactt ttttctacct
    18901 caatccccca agaatctttc ttgaagtgat tgatgatcaa tggaataacg aacatgacca
    18961 ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc tgtacctaat gtagcaaata
    19021 cgacaacgat gatagagaag atcagcatgc agctgactac ccaagctaat ccgtttccgt
    19081 ttttaccaat tcggtaagga cgttcgaatt ctggatgttt cttacgcatt ttaagcaatg
    19141 aaacagcaat caaaatgtac acgatacaat aaataacagt tgtagcattt gtaagtgtca
    19201 agaatacagc acttacgtca tccattacac cataaagcaa agcaaataat gaaataacga
    19261 ttgattgtgt tagaacgaca tttctagata cgccgtattt gttttcttta tggaatccga
    19321 attttggtgg aaggaatcct tcacgagcta cttggatgat tgtttttgat ggtccagtaa
    19381 cccaagctga taattgtaaa agcacaccaa taaataccat gaagctgaag atatttccga
    19441 tcacttctgg taagcctagg atttgacagt caatcaagat aggctgcgtg atatttgcta
    19501 attccatttt tccatctggt acgacatttg ctacaagcat cgcattgatg acattcaata
    19561 aaactaatcc gatcaaagcg ataaatacgc cttttgtata attttttgtt gcgtctttca
    19621 aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa aataaatgtg ataccagcta
    19681 agtatttcaa tgaatcaata tgttccaagt cagggaaagc ttttgaccat gaaaagtcac
    19741 ctagatagcc gtttgagact agtccgactt tgaaggctgc caaaacacct aaaacgaaca
    19801 tgatgactac tgggatatat acccctagcc atacaccgat gttcccgccg acttttgcca
    19861 tatcgaattt caagttcaag atcgtgatga tccagtagat aactaaaata caacctaaaa
    19921 cgaaccagtg attattgcca agttctacat taccaaatgt gtttcctaac aatggtccta
    19981 aagtagaagc gaccattacc atacctggga acatttgcac ccataatagc catgcaacga
    20041 caaatcccca tttgctgccc agtgctgttt ttacccataa ctgaggaccg ccttcttgag
    20101 gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg accggcaaaa aacaaaactg
    20161 caaaaattga ataaaaaatc aatgtccaac cgactgcggc taacgtagga atactccgaa
    20221 ccgttgcaca cagtgccatt gtcagaccga taaaggtccc aagacccatt ttacttactt
    20281 cagatttact caccgttgac actctccttg attttttgga taaaataacg gtgtctttga
    20341 agtagtttca gcaataaacg aaacaattca gcgaatattt gaggggattc tttgagtcta
    20401 ttgcttgaag ggtttcttca aagacaacct caaaacaaag ggatcgaaga gcttaggtct
    20461 ccccttgctc ttcagatcca aacatgcgtc agaaacagtt agtattcaag ctgttttcta
    20521 acatgattac cttgcttatt ttgcttcgct tgccaataat tggtctgcgt agattttttc
    20581 caatttttct tgaagagctg ctttgatttt ttcagcatat tcttcaaagt gttcttcttt
    20641 gttcatgtat ggtgtcatta cgctggcacg caatacagtt acttttccgg cacggttcca
    20701 ttcttcatct gagaatccta gttggttcac aaattgtaat gggctgttgc catagtctgg
    20761 gatagcaaag tcagtatgtg aagttaagaa ctcattaccg taaatgcttc ctttcacgta
    20821 tgaagaataa tcatatacgt catggttcaa tttattcatt gcaactaagt catcgttgcc
    20881 tttttctttg aatacatagt ctaccatatt gaaatctgga taagttaatg gatgaacttc
    20941 gatttctttg tctcctactt taaatgacaa gtcattcaag aagttgtaga aacgatgtgc
    21001 accttcgatt gatgcaccca ttaatttacc atatcctgtg acattcaaag gtaagacatg
    21061 gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt gaaccttcca agatataagc
    21121 cccaagtaat gctgggatat ctgcgccttt ttcaaataca tatgtcgcaa agtaagagat
    21181 aacgtctctc atgcggatat ctttgatgac gataccaccg gcagaatatg gaacatagcc
    21241 cattttatgt gggtcaatcg ttacagattc tgcttcttcg attgctttat atgcactgtg
    21301 tacttcttct aagatataat ctttgttttc tgtaaagaca ttgtatttgt aatgaacatc
    21361 ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct aagaaaatcg cacggccgta
    21421 tccgccataa gcagcatcta cgtgtaagta gaagtaaata ccgtcttttt ctaatacgcg
    21481 acgcaatgca acgattttat cgataccgtc gattgctcct tcttctgttg atcctacaac
    21541 accgactaca cctaggattg gtgttttttc agctgctaga ccacgaacga ttttttcaag
    21601 ttcgttgatg tccatgcgat agttgtggtc aactggcact ggaatcactt ggtctaaacc
    21661 gataccgatg atgtcggcag cttttagcca tgaatagtgt ttagtttgtg gaaccaacca
    21721 ttttcctaat ttttctaaat gttttccgct acgtgctgag tgagctttga tttcgtcgat
    21781 tttttctggg acgctgtcta acagattcat gatttcttct gttgacaagt tcattaattc
    21841 ccaatcgctt tttccagcaa ctagttcagg tgtcacttct ttcattgcaa gcggcaatga
    21901 tttgatgttg cgggcatacc aaagtccttc taagtttgct aaagaaccat cagcaacgat
    21961 gtggccccag ccatctttat agctcattaa ttttgcaaat tccattccta cttcttcttc
    22021 catttggctt gttgcaggag aagattcata ggcaacgtta ttaccattcc atagcatagc
    22081 aaaattgtac gctagtaaag atggcatcaa tgtttcagag ttcatatgac cccagtaacg
    22141 tccggcatta tgccaaggaa cggaatgggt acgcatccgc gctgaaattt ctgatagaac
    22201 atcctttgtt ttattcacag tgtgttcaaa actagcactg cttttttctt ctggcgtaat
    22261 aataggcatg tcttgaggca tgtagttttg acgccagcct aaatgttcat ccaccaattc
    22321 attcaacaaa gctttataga tttggccgtt ctcagctttg tccccaatga atagggcatt
    22381 taggtttaga tctttcgaca atgattcact catagtgaaa ccctcctaaa aaaatattta
    22441 aattttgtcg agccctacat tacacctgtt cttctattct gtaaactaag ttttggcctg
    22501 atttttaatc atattttaat atgtatcaat tatccgaatt gttcacactt ttgaaataat
    22561 taaaaaaaac gagggaattt acaaatgtaa actccctcgc aaagattgta ttaacgacaa
    22621 ccagcgttct attttaggat gggtttcaaa attggaatgt tgtaatagta tttccctttt
    22681 tcaaggtaaa aacttactaa attctaatca attactttgg ccaaaaagta atttttctta
    22741 ccttttcgaa taaccacaaa cttaccatcg aattcatcgg atggatttat gacaaaattt
    22801 aaatcggtaa cgcggtcacc gttaatactt atagctccgt ttgaaacatc ttcacgtgct
    22861 tgacgtttag atggttcaat ttttgtggaa acaagcagtt ccacgatatt ttcaggtgtg
    22921 cttgaaattt caacgttcgg cattttaccg aatccttgcg cgatttcttc cgcatttagt
    22981 tctttaatat ttccagagaa caatgcttgc gttatttttt gcgcttcttt caagtcttct
    23041 tcactatgca caaatcttgt aacttcttct gccaaacggc gttgtgcttc acgtttctca
    23101 ggttctgtct ctactttttt cgctaagtct tcgatttctt cttgagacaa gaaagtaaag
    23161 aatttcaagt atttcactac atcgcgatca tcttgattca accagaattg gtagaactca
    23221 aatggtgaag ttttcttcgg atcaagccaa acggcaccgc ctgcagtttt tccgaatttc
    23281 gttccgtcag ccttcagcat caatgggatc gttaaaccga acgcttctac ttctggtcct
    23341 tctaattttc ggattaggtc taatccagct gtgatatttc cccattggtc tgctccaccg
    23401 atttgcaatt gcacatcgta tgttttatgc agatgcaaaa agtcgataga ttgcagaatt
    23461 tggtaagtaa actctgtaaa tgagattcct acttccaaac ggctggcaac gatatctttt
    23521 gctaacatcg tgttgatgtt gaagttttta ccgtagtctc tcaaaaattc aagtaaagag
    23581 atttttgaca accagtcgta gttgttcaca aatgtgatat ttgcatcttt accaaacaat
    23641 tttctcattt gattggaaag tgcgtccacg ttatgttgca cttgttccat cgtttgcaac
    23701 acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc ctgttccgcc gccaatcaga
    23761 atatatggat gatggcctgc caattggaat cttttcatca tcataaatgg aatcaaatgt
    23821 ccgatatgca tcgaatcacc agttggatca acgccgcaat aaagtgaaat gctcttttct
    23881 tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat taatggcgtc gcgccaagtt
    23941 aattcatcaa taatattcat tttactgacc tccgtgtagt ttttttatga aagcaggaac
    24001 ctgctagcat gtgtaattaa ttatttcttc aggataggat aggaaataaa aaaagtccct
    24061 tggcatataa gccaagggac gaaatctcgc ggtaccaccc aatttgtatg tctagatatc
    24121 caacatacct ctcgaacatt taacgctgtc gcgtggcaat cactgccaaa gactcctgaa
    24181 atgtaattcg ctttaaagca tgtactggtt tgcagcgacc accagctttc tgaaacattg
    24241 gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt attaatatgt gtctatcata
    24301 gagcgcaact ttttctttgt caacagaaaa aggatatttt ttttaaattc ctcatttttt
    24361 cttttttgaa tccgctctaa ttccgaacat tttattttca attattgtta aaagaaataa
    24421 ctttttctgt attttttctg caatttatcg agaagtcact ttactttgcc tcttattctt
    24481 tcttcgttac aataaggaat gggacaagta aaaaaatgaa aaagcaaggg acgaggtata
    24541 tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc tttaactatc gttattttaa
    24601 ttctaagttt gttttttgtc atccgatcat ctacaaaaaa agaaccaatc attcctgaaa
    24661 aaaaaataac agaaaaagca aaagaaccga cagatgataa gcaacaacta gttactcaag
    24721 caaaaaatct ggctcaaagc tacgattatg accaagcgat tgcgttattg gaaaaagata
    24781 gtaagaacgg atctactgaa acacaacagc ttcttcaaac actaaaaaag gagaaagaac
    24841 aactgatcga ctggtctgat cctactcaga tctctcatgt ctttttccac agcttgatcg
    24901 tggacccgca aaaagccttc cactctcaac aggcacaagg atacaaagat tatatggtga
    24961 ctgttgaaga attcaaccgt tcgatcgaac aactttatca aaatgatttt gttttagtta
    25021 atttaaatga tttgatccaa aaagacgaac aaggaaatct ttcctttaac gggcttgcgc
    25081 tacctgaagg aaaaaaacca ttggttcttt cacaagatga tgtcagctat tatgaatata
    25141 tggataattc cggttttcct agccgattgg tcgtatcaaa taacaatgaa atcaaaaatg
    25201 tctataaaga gaaagataaa gaagaaatcg gtgactttga catggttcct ctgatcgatg
    25261 cctttgtcaa gaaacatcct gatttttctt atcgaggcgc aaaaggtacc ttggccctga
    25321 caggttacaa cggagtttta ggttatcgaa catcaaaatc tcaatatgga gacaatgaaa
    25381 aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga acagttgaag aaagacggct
    25441 ggagctttgc ttcccataca tggggtcact tgaatatgac tcaagcttca ctagaagatg
    25501 taaaaaaaga caatgaattg tggcagcaag aagttgcacc tatcctagga aaaacaaata
    25561 ttctgattta tccatttggt gcagacatca gcgactggca gccttattca gcagaaaatc
    25621 aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt ctgtaatgtc gatgcttcta
    25681 caccagcttg gggacaatta ggtacggatt tctatcgaaa tgctcggatc aatatcgatg
    25741 gcattcgttt tcaatctgat ttagacggaa aaaatcctat tcttgatcca ttcatcaatg
    25801 tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg attttacgtc atttatgaac
    25861 caaaaaagcc gaactgacat tttatcgtca gtttcggctt ttttatgctt tatttcttct
    25921 tcggtgcgat tcccttgatc aggacttctg tccattctgc tgtatatgga agcagttttg
    25981 cgtatatctc cggcttagaa attccttcta ccattgcttg aaaatagagg atcaagaact
    26041 catccgaata tttcaaatcc acttttcctt ctttccgccc tcgatggaaa agatcaagca
    26101 tgatactgaa actttcttgc gtatacgcat ccatcgcgtc agaaaattct tttttttctt
    26161 tttttgtata gtatgtcatc aaatcgatat aaaattgttt gttcactcga ttgagataac
    26221 ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa tggaagattt tccgcaatca
    26281 acgctttcgc ttcttgccct aaatcattca taaatgcaaa aaatacttca taaatcagcc
    26341 cttccttgct gccaaaatat ttaaaaatcg tcgctttact tacctgagcg ttctcagcta
    26401 ccttatccat tgtcactcca gcggttccta tgtctgtgtt catcaagtca aaagctgctt
    26461 gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa accgttcatt tgtagagcct
    26521 cccgtaattt taaactttta taatatatat agttcaaatt tattctgagg tttattataa
    26581 ctatctattg agaaagtatc aagctagcaa ttccttgttt atgaattcat tgacagaaat
    26641 ttataaaaag aatattatgt actaaaataa taaaaaaagt tttataaaaa ggtataaaac
    26701 ctttttatta aactatttaa tcttgtttag tatatatttt tagaaaaagg agtggggttt
    26761 ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag ctttggtcgt ttcgaagctt
    26821 taaaagatgt ctctttcacc gtacattcag gtgaagtcgt tggatttatc ggaccaaatg
    26881 gttctggaaa atccacgacg attcggacac ttttagggat tataaaaaaa gacggcggaa
    26941 cggcaacgat ttttgggcaa gatgtatggg aagatagttt atctatccat gaacgtcttt
    27001 cttatgttcc aggcgatatc tctttgtggg gaaatttgac tggaggagcg atcatcgatc
    27061 tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa agatgaactg attcaacgtt
    27121 tctctcttga tcctaagaaa aaagcaaaaa actattcaaa aggaaatcgc caaaaagtgg
    27181 gcttgattgc tgctttggct gttgattcgg atctctattt atttgatgag cctacttctg
    27241 gtttagatcc tttgatggaa acgattttcc aagaagaagt tgaaaaattg aaagaacagg
    27301 gcaaatccat cctactttct tctcatattc tcagtgaagt cgagcgacta gcggataaag
    27361 tcgtgatcat acgcgaagga acagtcgtag aagaaggaac actcgatgaa ttgcgtcatt
    27421 tgactcgttc gaccatcact atagaaacag aagcagagaa tagtcactta gcacaattga
    27481 acggtgtcca tgactttgtg caaaaaggtc acctcgctca atttgctgct gataacgaag
    27541 ctttcgatgt gattttagct gaagcgtcca aattaggtgt gaaaaaattt gaatctattc
    27601 caccgactct tgaggattta ttcatgagac attatgaagg atgaggagga agcgaaatga
    27661 ctgataaatt ttcacgctgg tccacccttt tttctcaaaa ccttagccgt gactggaaaa
    27721 agatcctgat atggctagtc ggtgtcactg ctttttctgg tggttttgtc cctgcgtttg
    27781 aagaattgac ttccggacaa ggattagctg gattatacga aaccttgcaa aacccggcaa
    27841 tgattgcaat ggtcggacca actccagctt cgaatgctgc tgagtatact ttaggagcga
    27901 tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc tgctgtgatc tctgttttac
    27961 acgtggtgag ccaaacgaga aaaaaagaag aacttggtct gattgaactt attcgttcct
    28021 ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc tgaaagtatt ctgatcaatt
    28081 tgcttttagc aatatcgatc agctgtacga tgattttttt ccaagcagat tcgatcactg
    28141 cagaaggtgc ttggcttttt gggctatctg taggtagtat ggggattttg gggagtgccg
    28201 ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag ttcgaatggt ctgtctttag
    28261 cgtttttagg tggactttac cttctacgag caataacaga tgtagctgat tcctcccttt
    28321 cgatgtggaa tcctttaggc tggtcttatt tgacctatcc atttacaact aacaactgga
    28381 ttcctttgct ttatttgttt ttgttcagtc taatcttgat aatcagttca tttgttttag
    28441 aggaacatcg agatttgaac accggctata tccccgaacg tgaaggaaaa ccgtatgcta
    28501 aacgttctct tttatccttg cgtggtttat tattctatct aaataaaggg atgacaatca
    28561 gttggcttat cacttttggt ttgatggggg cggcatacgg ctctatctac gggaatatgc
    28621 aacagttttt agaaagtaac gacttaatga aacagatgtt tatccaatct ggcgtctcaa
    28681 ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat ttgtctagct gctattttac
    28741 caattgctag catcaatcgt ttatttacag aagaaaccca cggacgtatg aatcagctgt
    28801 tcgcaacaaa aatttctagg aaccaacttt attggaccaa tatgttacta gctattctat
    28861 ttgctatatt ggcaatcctg attagttctg gtgcactagg gctttctgct ttggcttcga
    28921 tgaaggatgc gaaagacatg gatctattga tgtttcttaa agccgggttc aatttcctgc
    28981 ctgccgtttt agtgtttatc ggaattagtg gctttctatt cggatggctt cctcgaatgg
    29041 gtaaacttgc ttatatctac ttagcttact cctttgcact caattacttt ggcggaattt
    29101 tggatttacc agaatggttt tccaaaacag ctgtccctag ctgggttcca cggatgccaa
    29161 acgattcttt tgatctttcc gtgttcctta cgttatcatg tatcagcctt gcactgttcg
    29221 tccttggttc tattggttat aggcgaagag acttactaga aggataataa aaaagcatgt
    29281 acaatttcat gctagcctcc aaatctgggt caaaaatcca acatttggag gtcatttttt
    29341 tatttacaag tcttctaact gctctttatc aatgatccag acttctcgat tctttctttt
    29401 tacataacca ttctcctcta ataatttaaa ttttctagat aaagtttcgg gggtcgtacc
    29461 gataaatgca gcaagttctt tcattttcat tggcaggaaa aagtgatcct tccctgtcgc
    29521 tttagctaag tcaagcagat agacagcgac tcgttcttct actttttcca tcatcaaaaa
    29581 agttgtctgc tgctgtactt ttgttaattt ctcaacgttg attgctaaaa gtttcagact
    29641 aagggcggga taatgcagta agagttccga gaaatctttt tgtctcaata tgcaaatttc
    29701 cgttttttct agtgcttcgc caaataaaga ctcatttgga acgcctagca actggctctc
    29761 tccctcatat ccgcccggct ctattactcg taaaagctgt tcctttccgc tagcagataa
    29821 ttgatacact ttcatactgc cggaggccac gatcactaat tgaggttctc cattcggcgc
    29881 aagaatctgt tctccttttt ccacatagtg atgtttgaca agtgcgtgta ctttttcttg
    29941 atcttgctgt actaagtgat tgaacaaagg aacaaatgaa acacataaat gatcatgact
    30001 cataatctct cctccttcta aacagctatt tctaataaag aaacataact tcccttattc
    30061 caactgataa ttttctttta tcatagcata aacgtcaagc gaaccagata ccagcaaata
    30121 gagtggattt ctttttgtcc cttgctttat tttccttctt ttgagacctt aagtaattga
    30181 ttcctatcaa ttttttaaat atttactttt tgaataatca aaaacaaacc ttcactcgca
    30241 agccgagagt aaggtttgtt tttcgcattg cttatttcgt gttgtctatt gaaatttttc
    30301 cgcacctagc ttttgatata gtggtgctag ttcttgtaag gtttcagcgg ttatttgctc
    30361 aacgtgttcg atttcttgcg gatagaagaa tagtttaccc atttgaaagg ttcggtctct
    30421 catcgctttt ttcaactctt cttgtttctt tgtatcggac aaatagtcag acaaagctaa
    30481 gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa taatccttga tatcgactgt
    30541 tttgctccac tctttcagta tcgcaagcaa ctggttatac gctgcaatgc taccatatcg
    30601 ttcttcgctt ttataatgtt ggtacatcaa atagatttgg tattgttttt gattcaacaa
    30661 aactgctaga caggcattct cgtcttgacg agattcgctt cggtacgcgc tccaaaaatg
    30721 actgcgtaaa ttccagccat tcgtccagct ttcgatttta ggtttgctca tgccgtacat
    30781 atcaggcaaa agtgcagctg tctggagctg aatctctttc catttttgcc agtgttcttt
    30841 atattctttc ttgatttgtt caatcttatc tgttggatgg ctctctttga gctgtttgaa
    30901 tgcgtagatt ggtcgatcaa acaactgata atctgatttt ccaagttcca ttgtttcgcc
    30961 tctttccgtt atcttttgta ttctacctga attttgcgat atggacaaat agaggttgtg
    31021 aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca aaaaactgcg atgagcatca
    31081 ccgcagtttt attgaactta aggttgattg atcacatcta aaagcgttga aaataaaatg
    31141 gcactagaag cagctcctgg gtcgatatgt ccgattgcac gctctcctag ataggaagct
    31201 cgtccttttt tagctaccaa gactttggtg tgttcttttg cctggtcaat gatttctttt
    31261 gttaaattcc ctgccttcaa tgcctctgtg actggcagcc aaacatctaa catcgtttta
    31321 tcgtcagctt ctgcctttcc tcggctttga atacccgctg ttccctgctc gatcacttcg
    31381 cctaattttt cttgagaatc aatagaatcg acctcttttg tcgctttcat catgttcatg
    31441 aatgcagatc cgtataacgg accagaagcc ccaccgactt tcgatatcat cgccatagag
    31501 aatactttga atgtatcgct gattgtttca ggatgatcgg cttgaaaagc ttcttcataa
    31561 gctgaaaccc ctcttcccat gttatttcca tggtctccat cgccaatcgg ggtatctaga
    31621 tcactgagat attgtttgtt ctgattgatt gcctctgcaa attgcgtgag ccattcttgg
    31681 atttcttgaa cggttagttt catattatca cccttttatc ttgagatttt ttaccatgaa
    31741 atcgtctgta cattgctttc taaagaatct ttccattgtg tctccgtcaa gtcgatcagt
    31801 gttagagaaa gaccttgcat atctagtgag gtcataaaat taccgacttt tcggaaaact
    31861 acattgacgc ctctttcttc caacagagct aaaacatcat tcatgaacac gaactgttcc
    31921 atcaaaggcg tcccgcccat tccgttgact agtacacctg cttcttttgg taaattcgag
    31981 taatcatcta aaatcttagt aacaagttcc tttgccagta ctttagaagg ctgcatcttt
    32041 tctcgacgat agcctggctc accgtggata ccaacaccat actcgatctc atcttctcct
    32101 agttcgaatc ctggtttccc tacttctggt acagttgctg ctcgaagcgc tagaccgatc
    32161 gtcttagttg cctgtacgac cttttctccc agttctttca actcagctag ggaagcaccg
    32221 tttcttgctg catcgcccac gattttgtgt acaaggacag tacctgcaac tccgcgtttt
    32281 ccagcagtat acgtactatt ttcaacggcg atatcatcat ctacgatcac actttctact
    32341 tcaatatcgt ccatagcagc taattccttt gccatatcga agttcaatgc atcccctgta
    32401 taattcttaa cgatcagcaa aactcctttt ccactatccg cttccttaat agccgtctga
    32461 atctgatctg gagttggaga agtaaacaca tcaccaagca ccgctgcact taacatccct
    32521 tcccctacaa acccggcatg ggatggttca tgtccgcttc ctccaccaga aaccaaacca
    32581 acttgttccg ctttctgatt cttggctaca acacgggaag attctacacg atgaacaagt
    32641 tctggatagc tttttactaa tccttccagc atttcttcga caatattgct tggatcattc
    32701 atgatttttt tcaatttatt cccatccttt ctttccggca ttttgtgctt ctgttaagat
    32761 ttgctgagta tcatctgtta taccggcagt gatggctgct gcaaaagctc cttccactaa
    32821 aggcgcatcc accagatgat aacgttgctg ctgctcttct tccaacatgt cttttgccag
    32881 ttccgagttc aataccgcgc taccgatatc cgcaaaaaca aaataactag ctgcatcgct
    32941 tgattcattg actgcttcta cgattttcat cgggtctgat ccgatctcgc catttattcc
    33001 gccgagagaa aaaatttgca cctcttcgga tttttgcatc tgctcaatca tttctttaat
    33061 accatctgtt actttttgac tatgagaaat aagcaaaata ctttttttca caagaatctt
    33121 cctttctttt acatagttgc taacgattgg tttcgctttc attaatgaac aattcaagta
    33181 tataataaat ttctaatttt gggaagatat gtgtagtgct tcttgaagtt tgttatactt
    33241 taattaaaag agaggagtga tcgaatgatt acaacaacaa cgaacagtgt agagggaaaa
    33301 gccgtagtag cttataaagg gatcgttttt ggtgaggtca ttactgggat ccatgcattt
    33361 aaagacttgg aggcggggtt gcgaaatatc tttggcggcc gctcaaaaag ctatgaaaac
    33421 gaactaatgg gtgcaagaga agaagcactt gccgaaatgg ccgaacgtgc aaaagcatta
    33481 ggagctgatg cgatcattgg cgtaaaaatg gactatgaag tattaggcag cgacaatggc
    33541 atgctgatgg tcacatgtag tggaacagct gtctccacga gataagtagg ggacatctgt
    33601 tttaataact aaagagagat gggacatgta gtggcactca agaattagat ttttcagtct
    33661 aacttcgagg tactctacct tcccatcctc tcttttttat ttctttttgc ggatgattcc
    33721 gtaagctgct gcgactaaca cacccgcccc tgctaaggaa taactgacag aagctgtttt
    33781 ttcatttgtt tttggcaaag tctgacttgt tgatttggat ggctctgtcg tttgctgaac
    33841 tgtttctgtc gtgcttccag aggtcactga tttttctcct gccgaatgca aaatatcttt
    33901 tattcgttgg aaactagctg ctaagtcagc tttttttgct tcaaaatcat tagcaactgt
    33961 ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc gctgtgatat ccgcttgagt
    34021 ttgacttagc tttccagaag ctgtttgagc aacctgattt aattgctgat tgaattcttt
    34081 tgcttgttgg atcttcccgt caagatctgt ttggacagct tccgcttttg ctttcgcttc
    34141 ggcaagcttt ttctcaagtt gtgcacggtc atttcccact ttatttttaa gatcagctaa
    34201 atcttgatcg attgctgctt ggtcttcttt caatttgttt tttgcagctt ctaatgcttc
    34261 ttcgagttct tttcttttat tttccaagtc ttgcaaatga tcgatttggt cagaagaggt
    34321 agaactattt attgatgtat cttctgaaga tgaaatcgtt tctcctgaat tatctgtcgg
    34381 tacattggaa gtcatttctg tttccgaggc accgttttca gcaccagaag agcttatagt
    34441 tgttgaaggt tctgtttgag caggttcaga agtttccgca aaagctgtct gtcccaataa
    34501 gaaaatactg gaaacagcaa ttggccagat taacggcttc attctttttt tcatcacttt
    34561 tcccatcctt actacttttt aactaatttc ttattataag caaaaatgtt tggaagaacc
    34621 ataaacacaa caggctgttt gtctaaaaaa cggtgcatct tttatttttt tgtacgttcc
    34681 gggcttttcc atgtcccgct tccataacct aaaatcgttt tgactgctgg aatgaaagtc
    34741 gtcacgatcg ttatcgcgtt cactatccag aacaaaagca tatatagtgg tgcgaaaaga
    34801 aggtatttca attttcttcg tcgatcatct acaatcagcg aagctagcaa ctggaaaaat
    34861 cctgcgacca tttcaaagca gatgaacaca aaactgaatg taaaagtaat ataaatctgt
    34921 tcgtagtttc cttgatatac ataatgcccg attaatccag caaaaagaac tacggaaata
    34981 caaaagaata ttgaccagac aatactcaat gtttgatcaa tgaagatgat cgttcttcca
    35041 atatgtttaa aaggatggag catcactttt ttgaaattcg tcagccagac ttctgttcca
    35101 ccttttgccc aacgttttct ttgtcgatat agcatcttca acgtcacagg tacttccatg
    35161 aaaaagataa tctcaggagc aaaaacagat aaccagtcat ttaactgatg atcccaagca
    35221 atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc ccacatcgat caaggcttct
    35281 tttcgataca tcgtatttgc tccactgtac gcataaagac caccgaaaac agctgactgt
    35341 gttcgtttaa tgatgcccac gatacttgag aattcaaccg tttgagattt tgcaatcaat
    35401 ttggtacgat tttggacatc catatttgca gtgaccgcag caatatgtct tgcaccaggg
    35461 cgaatgaaat aattcacata cctaatcagt gcatctggct ccggtaccgt gtctgcatca
    35521 ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga tattaaacgc gtgggctttt
    35581 cctttatttt tttcgatccg aaccacacga agattagcgt attttttcat cagtctagct
    35641 aatatttctg gtgtttggtc cgttgaacca tcgtctgtca ctaatacctc atagttgtga
    35701 taattcaatt tcgtcatcaa atactcgatt gtatcttcga tcacaatttc ttcattatgg
    35761 gctggcacca tgatcgtgat gaacggttcg acactcaagg gaacatctac ccattctttt
    35821 tgcttatgtt tgtataaaaa cacataacac cagacaccaa taaaccatac aaatccgcct
    35881 aaaatcggat aagaaacgag aaaaaataca taatagacaa acaagtatct aagaataaat
    35941 tacctgtatg ggtgattgag atcatttcag ttccacccca ttttctttat acaaatctgc
    36001 aatgaatgtc gtatctagat ttttctcttc ggagacagaa taaaaacgga ccgtttctct
    36061 ctcttctttt gttccaaaac gttccgtata cacttcttca agtaattctt gtcttttttt
    36121 caatttttca tcttcatatt caaaagattt atttactttt ttgtatagat ttcgattgtt
    36181 ccacctagtc aatacaaatg aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc
    36241 gattaagaaa tacagcattt tcttttctga taaaaaagcg tacaaggaaa aatccttggc
    36301 tctttctggt ctcgttaatg ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca
    36361 tcctaaaaga gccactaacg tttgccaaat ctttaaccag taattacctt ttgaaaaata
    36421 cttatctttc accaaaattg atgcaacttc ttctcttgtt acttttttca tatcctcacc
    36481 tcacgttttt ttgttacgac gtaggagttt accatacatt tcatttcttt tcacataaag
    36541 tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata
    36601 ttttgttctt tttatcagtt gttcttttta ttttttatct cttaattatg ttgctctttc
    36661 caatacgaca atgcttttca ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct
    36721 ttgctcatct ccatcgtttt caaatggata taagattaca tgatcaaatt ccagtccttt
    36781 tgctagactc aaaggaatga tttggcagtt ttctaatcca tatgaatttt ttttaatcga
    36841 ttcttgtagc gtttgggttt cctcttctgt ttgggtgata actgccgctt tttctccttt
    36901 tagagaaacc aaaatctcag ctagtatttt caacaattct tcagaggatc gaaaagagcg
    36961 gtattcgggt tcttttcctt gcggtcggat cgatacgatg tcgatcttct cttgatccac
    37021 agcataggtt ttgaaaagct ctgtgattgc tccgctagaa cggtaactat ttctaagatc
    37081 ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt
    37141 ggaagaatta aagattgcct gattctcgtc acctactaaa gtaaagtgag tttttggaaa
    37201 taattcgatc aatagaccta attgtgccag ggtataatct tgtacttcat caatcaatag
    37261 atagcgaaat gctggtacat ttaccttttc aacaagtaaa tgacgaatag ctaatataat
    37321 gactgcttca tcaacagtac gtgtgggttg ccaagcataa gctgagccat atcgtttttc
    37381 ataaattctt tcaaacaact gatgctcatc cacccatgcc atttcttcga tttgtctggt
    37441 tatttttcga tatttttttt gtaaaagttt ccttgcataa gctacaatac tttgttccga
    37501 atcatcagaa atcaatttac cgaataattc caactgccta tcttctgtca acgagagtac
    37561 ctgattctgc acagcaggac tttttgcttg tttcaaaaga tgattcttcc attcgctcat
    37621 cagtgccttt ttcgttgctt gaagcctatc gatcaattta ggatgagcag gaactttctc
    37681 atatattttt tcgatcgttt ttttagaaag gactgttttt ccatttttag taagtccacg
    37741 gataaaattc ggatgatcta aaaacaaagg atctgattgt tttagattat cgataaactt
    37801 tttgctacgc aaacgttctg tctgctccga tacattttct cccgtgatac gtttgaaata
    37861 agcctcttca ccttctattt cctctgctga aagctgtgaa accagctgta tgatggttag
    37921 attcagtgga tttctttcac ctaatgatgg taggacattc gaaatatagt cgataaaccg
    37981 atgattaggt gatagaatca gcaagtcatc ggatgtcatc gtatctcgat ataaatagag
    38041 cagatacgca atacgctgca tgatagcaga ggttttacca cttcctgcta cgccatttac
    38101 caagatattt tgacttttca catcccgaat gattgtgttt tgttccgatt ggatcgtagc
    38161 agtgatagcc ttcatttcat tggtctcatc ttggcctaag acttctaata atacgtcatc
    38221 ttgaatagct acagacgtat caaaaaactt gaccaaacga tctctttcca aaagaaattg
    38281 tcgtctttct ttaataaccg tatcgatttt attttgacga accgtataag aagatggacc
    38341 aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc caatcataaa caagattttc
    38401 atctgcatca tctgtgaaat ttgctgtacc gatataaaag gattcttttg gctcattatc
    38461 cagaaagtct acaacgactt ttccaaagta gggacttttc aataaacgtt cgatctttgc
    38521 tagctgattt tctgcgtttt tctgtttgat attcatctga tcgatttctc tatttttcat
    38581 ttcaatcatc gaaaaagtat ccaggttatc taagtaactg tcaaaattca atcgaagctc
    38641 gccagccatt tctttcaacg actggtttcc ttctattttt gccgtatcca tcgattcccg
    38701 cagttcgttt tttttatcaa ttagcttttg atacacgttc ttcagatatg cttcttctaa
    38761 ctgtttttgt gtgttgacca tacacattct cctctttttt taaagtggtg gaaccgatta
    38821 gtttacctct tttaagagtc gaatgcaagc tttttaacga gtatcatttg ttaggatata
    38881 tcagttcttc aggtcggaca gtttgatata aagaaaatgc accatcaagg ttttttacgt
    38941 ggtagccaga ctgcttcaag attctttccg ctagatagct acgtaatccg ctatgacagc
    39001 tgacgatata ttcttgttgg ctgtctaatt catttaatcg gtcccgaagt tcattcaaag
    39061 ggatatgttt ggcttccttg aatttgccct tttccaattc ttctgctgtt cgcacatcta
    39121 ataaaatttt tccttttgct agttctgtcg gcaattcatg ccattgtacg gtttcactaa
    39181 gcccttctgc aatgttcatt gctgcatatc ctagcatgtt tactggatct ttggctgagc
    39241 caaatggcgg tgcatacgtc aattctaatt ccggtaaatc gaagatcgtc aaatgccctt
    39301 tgattgctgt tgctaaaata tcgatccgtt tatccacacc ttttgcccca acaccttgtg
    39361 caccataaat ctcgccagtc tctggatgga agatcaactt cagtaaaata tctgttgctt
    39421 ctggataata gcttgcatga tcttttcctg atacatgaat gacttcaaaa gacagttcgt
    39481 tttctttggc tgttctttca cttaaaccgg tggatgcggc agtcattcca aacgttcgta
    39541 taattgctgt tccaataccg ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa
    39601 cttgtctacc ttgacgattg gctggtgaag caagagaaat aagtgcatct tttccagtga
    39661 tttgctgttt tacaacgata gcatccccca ccgcataaat atcaggaata ttcgtttgat
    39721 aacgttcgtc tactaggata cccccgcgta tgcctaattc gattccagcg gtcactgcta
    39781 gctggttatc aggacgaaca ccaatggata aaataactaa atcagcaggg atggtacgcc
    39841 catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa
    39901 tcacttctac tccttgatga gacaattctt tttctataaa agctgccatt tcttcatcca
    39961 gtgttggaag aatatgtggt gcttgttcga tcaccatgac ttctaaacct cttcttttta
    40021 ggttttctgc catttccaat ccgatgaatc ctgcaccaac gatcacggca cgctttggct
    40081 gtttttctaa tgcatgcatc actttatcga tatctggtac atttctaata gaaaaaacat
    40141 tgtcggcttc tgttatccct ggaatcgatg gaacaactgg ttttgcacca ggagataaaa
    40201 ccaatgcatc gtactgttca atgtgttttt gaccctcatg tacgacttct accgttttgt
    40261 tttcgggaaa gatggctgtc acttcatgat ttggacgcac atctaaacaa aaacgagcgg
    40321 ataatgattc tggcgtttgg acaagaagat tttcccgttc actgatttct cccgaaagat
    40381 aataaggcaa gccgcaattt gcaaaagaaa catacggtcc tttttcaaaa acaacgattt
    40441 ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga cattccacct gctacacctc
    40501 cgacaataac gattttcatc ttatttccct cctactgttc gatcaaacca ttgattcatg
    40561 ccaccacgta cattcactac atcataacca gatttcttta attctttagc tgcttgttta
    40621 ctcctcatcc ctgattgaca aatcacatag acggtctttt ctttgtctcc atcatatctg
    40681 tttatacttt gtaatggaac atttttagct ccttttatat gcccgccccg gtattctgca
    40741 ggtgttctaa catcaagtaa cgctggattt tccaaaagct tttcctttaa ttctgggact
    40801 gttatcgatt ttattgattg aaatagcaaa ttcattttct gcacctcatt tacattttta
    40861 taaatacctc atggggtata ttaaacagga gctttattaa tatgtcaacc agaatgactc
    40921 ttcatttttt tcactacttt ttatatttgc ataaaatacc gataaggggt attatctaat
    40981 tataaaaata gaggagaatg tgttatggag attgataata aaaatatcct gaaccgtttg
    41041 aaacgtacag agggacaaat tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt
    41101 atggatgtca tcactcaatt aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg
    41161 atcgttgcag ataatctgaa aaattgcttt gagaaacctg atagtaatcc agaagaacaa
    41221 gcggctaaac tagaacaagc gatcaagatg attattaaaa agtaaaggaa tgatttcaat
    41281 gaaagtcgtt ttccatattg atgaattaga aaaatggtca gaaacaggga aaaacgtaaa
    41341 aaacctgata aaagcttctc cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat
    41401 taccggttat ctagactctg ccaatgcaga attcttagat ttgcaaggag tcgtttttca
    41461 cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa agttctttac ctgaacaagt
    41521 aatcgttgta ccagcaggtg tccttgattt agttgaactg caatcccaag gttatgctta
    41581 tatcaagcct tagtcagtag aggttgcgac agaagtgatc agcttcaaga aatttcggct
    41641 tatttttcga agaagctact tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc
    41701 tgcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc gtttggttgg
    41761 gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaatcagc
    41821 ggtttgacct aagtattaga gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt
    41881 ttgagctaat accgaaggtc agctgattga acaccgttta aggaagagat tgtgaaaaag
    41941 tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gatttctaca tttttgcata
    42001 aatttggctt aataccgaga aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa
    42061 tcagcggttt gacctgagta ttagagaaac aaacggaaaa atagctgctc atattttgcc
    42121 gttttttgag ctaataccga aggtcagctg attgaacacc gtttaccaag aggttgtgag
    42181 cctaccattt aactccgaat cacaagaaat aatttcaaaa acaacttctc ctgtttttgg
    42241 tttggttggc ttattatcga gaagtagcta ctgggagtct atttatcaaa aaaaggaatg
    42301 cgacataggt cacacccctt ttttattttc tttgaaacat ctttcggtac tcgacagcat
    42361 acaagacaaa agattgtaaa agaccgtaag tgatgacggc aaaaatgact ctcacccaca
    42421 gcgttggtaa ttctgggaaa agcagcaaaa ggatcatgcc aacatagatg acagcagtcg
    42481 ctactttgcc ataccacttc gctccactta atttgatatt tttagatagc ccgattaatc
    42541 cagctacaaa cataaagcct tcttttatca agaatagacc aaacaacagc caaagccacg
    42601 gtcgatacca tgccatagac aaaatcagaa aaagttgtgt caatttatct gcaaacggat
    42661 caagcacctt ccctaagtct gttacctgat tatatctccg agcaatgacc ccgtctagat
    42721 aatcagacaa acctgagaca acaatgaccg ctcctgctat ataaaatgac tgtctcagca
    42781 caaaataaag ataaatcggc agcaaaaaaa tacggaatat cgataaaaga ttcggaatcg
    42841 tttgtatttc tttacgccag tctgtgctta atttcatttt atcgccctac ctcctcagca
    42901 ctcttttctt tttattttag cttacacggg gaataaaacc aatgatttca ctgtttttat
    42961 ttttctttac agccactgat attatgagct aatttgagat aatcacttgc tgttttcatc
    43021 gtttactctt ctttcttcct gtcgtttgcg catttctttt actagtactt tatgagcttt
    43081 ttttgatcct cgctctaggt cacgctgccg tttacgtgtt ttataatcga taaacagact
    43141 gtctaaatcg taacctgtcg ggtacagatc ttctgctgaa cgcaccagac gaacacgctg
    43201 acgaggcacg tggattattt ttttgtctaa atatacatca atcgtatctt cacctatatc
    43261 ttcataaact aacgcttctt tttggtattc attgacaaat atccgatcac cttttgcaaa
    43321 gagctggcgt tcttttttat tctccttcct atttgtaact gctgtcttag gaagaaattc
    43381 ttttttctgt gtccgatagg ctttttccgc caagtaacgc tctgcttgat ggatcaattt
    43441 catcgacata tccattttat gagcaatcca caaagcttgg ctttctcctg tttctccaac
    43501 ccgcaactga tatttaggac gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa
    43561 atcttcatga tctccggcga atttcttgat ttctccataa tgggtcgtgg caatgatcaa
    43621 tgccccttgc tcatacatag attccatgat tgcgatcgct agtgcagccc cttcatttgg
    43681 ctctgttcca ctgccaatct catctaaaag taccaacgta ttccttttta tctttttcaa
    43741 aatagcagcg atgttttgca tatgaccaga aaatgtactc aacgcatttt ctaaattttg
    43801 gtggtcaccg acatcgacga aaatctcatc aaagacagca agctctgtcc cttcttttgc
    43861 cggaacttgc ataccgaaca tcgccatcaa agtcaataaa ccgactgtct tcaacacgac
    43921 tgttttgcct ccggcatttg ctccggtgat aatcaagcct cgatagtctt taccgatttc
    43981 aacatccaat ggtaccgcat gctcaggtaa aaatggatgt ctgccttgct tgataatgat
    44041 atgttccgat ttattgattc taggtgtgct tccattgatt tctcggctaa attttgcccg
    44101 agcaaagatg atatctaaca ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt
    44161 ttctgcgata gcaccagtca atgccgccag gacttgatat tcttcactga tttcttcagc
    44221 ttttaacaac tgatagtgtt cattcaattt agaaatcaca gttggttcaa taaaaaccgt
    44281 cgttctttta tttgattcat cgataatcgt tccatctatt ttatttttat agctggcttt
    44341 gataggaatc gtatagtatt cgcctttttg aacaatcatt gcttcttgga tcatttcttt
    44401 gtttttcgga tgacgcaaga atttcagtaa ttttgactgg atttcttttt cgattagttg
    44461 taattgtttg cgtactttac gcaaattacg tgacgcgtcg tcactgactt tattattttt
    44521 tattttctgg tcaatcaact cttctacatt tatcaaatca ggaagatgtt tgctataagc
    44581 aaacaacaag ggtgtctggt attgattctt atcaaaaaac tttgtgatca tccgactgct
    44641 tctaagaaaa tccgcatatt cgatcaaatc ggttggctgc agaatcaacc cttttttgac
    44701 ttgttctgtc agtgcatcga tcctcggcaa ccccataaat ggaacatgct gactgctttc
    44761 taagatcaag cgtgcttctc ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt
    44821 cgagaccgtc atatcgctga ttcgtttttt gctatagttt ccaattgctc ttgcttggat
    44881 ttcctttttg atccgatcaa attgtaattg actaatcatt tcgttgtcca tattctctcc
    44941 ttttcttgaa gagatacctg tcccaaaata aataagggcc tagagataaa taaaaggata
    45001 gaacacagct taaaaaagtc atgtcctatc cttattagaa tacaaagata ttgttttcct
    45061 gacaggtacc gtttcataag cacaccaaat aaactttgtt tattggctat tcgacttact
    45121 gattagatac cagctacact cacaaaacat accccattct tctaggtctt atttattttc
    45181 cacttaacta tagcaagaca cattttggtt gtcaaacttt agtatgtttt atagatttac
    45241 accattaatg aatagacacc taccgcgata attgctccga tcattggacc tgcgattggt
    45301 acccatgaat atgcccaatc tgaagttcct ttattagcta ttggcaatac cgcatgtgct
    45361 aatcgaggac caaagtcccg agcagggttg attgcatatc ctgttggccc tcctaatgaa
    45421 agaccgatgg ctaagatcaa tccacctaca gcgaatacgt tcgttccagg tgcaaattcg
    45481 ttttgtccaa aagctagcaa cccaagaact aatacaaatg taccaattaa ttctgtgatc
    45541 acatttgctg gataattccg tatagcagga cctgttgcaa acgttcctaa aatcgcgctt
    45601 tcatctttcg ttgcattcca atgtggcaaa taagaaagcc aaacaagaat ggcccctgca
    45661 aaagctccta aaacttgcgc aatgatgaaa ggaacgacaa gattccaact aattgcccct
    45721 gtcatcgcca tagctagaga gactgcagga ttcaaatgag ctgggcccat gaatccagaa
    45781 atatagacag ccattgttac agcgagtccc caaccaaatg cgatgacaat ccagccagaa
    45841 gcctgtgctt tacttttgtt taaattcaca gccgcacaca caccatctcc aagcaaaact
    45901 aggatcagtg tcccaataaa ctctcccatg atttgagtca tatcactgtt catttatttc
    45961 tcccccttta gttcccgcag atctgattct gcaatcaatg cttctaaacg tttagtctgt
    46021 tgcgccttct cctcttctgt ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc
    46081 ttgatctcat caagctgatc tcgttcaaac aacagatgat ttgtacgacg aatgagataa
    46141 tcgcctgggg ccaaaaccat ttcttcttct aaaccataac gtaaccgagc tgactcagcg
    46201 agactcaaac caggataagg tgccatttct tttgctaatt caaagatacg tcgggcatta
    46261 gtcccgtaaa aatcagcgat ataagtagca tcttcttcct ctaaaccggc tgctactcct
    46321 tcttttgcta attctgtcac tgtttcttct aatttagttg gatcgaagtt tccacctgaa
    46381 atctgatatt ttttagagtc gatttctttc gtctctattc cgtattcttc ttttaataat
    46441 tggcgtatca atcgcaaagc tccttctgcc atcttacggt aatcggtgat tttcccacca
    46501 gataaagtca gcaaaccatc tggttctctt tctaaagaac tacctctgga aatcgtagaa
    46561 ggtgcttttt catcacggct gttttccaaa tggttcaaca cgtcttctac ttcagcacga
    46621 gaaactttgt tttccttata ttcacttact gtatcgacaa ctttattgaa gctcttgtct
    46681 gaaatcgatc cattatctcc accattgtaa tcagaaccag aattaccaat caacagtgga
    46741 cgaagtcctg cccagcttgc ttcgatatca ttcaatgtga tatttgcttc tggatagcga
    46801 tgattgatca catccaatag ataatctaca tcttcttgtg tgactttcgg atccgtaaaa
    46861 tctccttggt aatccgtatc tgtcgtacca aagtaagtct tgttttctct tggaatagca
    46921 aaaaccatcc gcccatcttg ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt
    46981 tttttcgcat ctacaactaa atggatccct ttagttggac gcattttagg agagactgga
    47041 cgcgtaaaat tcaagtttcg tactttatct acccaaggac cactcgtatt gatcactaat
    47101 tttgctttga tttcgatcac ttcatctgtc agcaggtcac gagctttgac gccaacgatt
    47161 tgatcgccat catataaaaa gccaaccgct ttcattttac ttactagata agccccatct
    47221 tcggcagcct ttttgatatt atcaatcact aaacgggcat cattgttgcg gaaatctaga
    47281 tagacacccg cgccttttag cccttctttt ttcaaaaacg gttctctttc caatacttct
    47341 tctggtgtca gcgtataatt ctcatatttg gttcctgtca catttgccag tttgtcataa
    47401 aggtccattg ctactttgac cgagaacata ttgaaggttg ttgctccttc gccctcatag
    47461 attggcaaaa gcattggatc tggttttggg atgtgtgggg caatactttg tacgactgca
    47521 cgttcaccaa ctgtgtcagc tactacttcc acatcaaatg tcttcagata acgaataccg
    47581 ccatgaacaa gtttggtcga gcgagaggaa gtcccttctg caaaatcctg catttcgatc
    47641 aatcctgttt tgattcctga tgctgctgac tggactgcca caccggcacc agtgattccg
    47701 ccaccgatga tcaacagatc caattcttct tgctgcattt tttgaatgct atcttgtctt
    47761 gtcttgtttg aaaacatatg gatttcctcc ttattcaaat gcttgggtag cttttactgc
    47821 ttttttccaa ccgttgtaaa gtttttctcg gcgctcttcc tccatttgaa cttcaaatag
    47881 cttgccagct tcataaaatt cgcggatctc gtctgtatct ttccaataac cgacagctaa
    47941 tcctgctaaa aatgctgccc ctagcgccgt tgtttccaag ttttctgctc gttggatcgg
    48001 tacattcaag atatctgttt ggaattgcat cagatattca ttgtttgctg caccgccatc
    48061 tactttcaat acaggaatct tgattccagt gtcatcttgc atcgtatcga tgatatctct
    48121 cacttgataa gcaatcgact gtaaagtagc tttaatgata tcttcttttg tcgtaccacg
    48181 tgtcaagcca aacatagccc ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac
    48241 gaatgcagga acaacataaa cctcatcttc gcttgtagag cgtttcgctg cgtcttctga
    48301 atcgctagct ttttgcaaca tttgaagtcc atctctcagc cattgaatcg aagaaccagc
    48361 cacaaaaata cttccttcta aagcatagta aacctttcca tttatgccgt aaccaattgt
    48421 cgtcaataaa ttatttttgg ataattgcgg tttttcaccg gtattcatga caataaatga
    48481 acctgtacca taagtattct tgaccatgcc tggttcgaat gccatttgtc cgaataatgc
    48541 ggcttgctgg tctcctgcca tccctgaaat tggtgcttcg cttccataaa aatgataacc
    48601 ttgcgtataa ccataaactt ctgagttaga agtgactttt ggtaacatca ctcgaggaat
    48661 atttagtaaa tctaaaatct cctgatccca atctagctga tggatattga acaacatcgt
    48721 ccgactggca ttcgagtaat cagtaacatg tgcttgtcca tcggtcagtt tccatactaa
    48781 ccaagaatcg atcgttccaa acatcaattc gcctttttca gctcgttctt gcgcgccttc
    48841 tacatgatca agaatccagc gtatcttcgt tgctgagaaa taggcatcga tcactaaccc
    48901 tgttttttcg tggaaaaatt cttgatagcc ttcatttttt aagttatctg caattccagc
    48961 tgactgtctg gattgccaaa caactgcatg atagattgga cgtcctgtat ctttttccca
    49021 tattactgtt gtttcacgct ggttcgttat gccaatccca gcaatctgat ttggtttgat
    49081 ccctgattcg atgaacgctc cggcaataac tgactgtaca gaattccaga tttcatttgc
    49141 atcatgttcc acccaacttt cttgcgggaa atattgcgtg aactcttttt gagaactgcc
    49201 gatgtgtctt cctttcttgt caaagataat tgctcgtgag ctagttgtac cttggtcaat
    49261 cgccatgata tacgttgatt ctgtcattgt gattccctcc attatatgca aacgctttct
    49321 ttaactactc ttatcatact actgttttaa aaaaagaaac tgtcaaaatt ttattctttt
    49381 tttctttttt tgacagtttt ttattatgaa gctatttttt aatcaattct tttctttttt
    49441 cagcgtctct attcttcttt tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag
    49501 ataatattca cactgttttt tgtttctctg gcaaaatgtg atcaccaaat catattcttt
    49561 accttcttca taacttccaa tctcaatacc gccgattccc ctcagttctc tgatcagata
    49621 ttgttgaaaa gcaatacgat aaatcggcag actttctaaa tcgattccaa tcactactgg
    49681 ttttgcaata taaaaatcga tcatcaaaag aacattggca tactggatca tcaggtaact
    49741 aagctcacta tcttcagctc ttttcctcgg caattgctgc accgctgttt cctgtaattt
    49801 cgccagtaac tgtgttaagg aatgcccgag cattttctgc tgctgttcca aaagccgatc
    49861 acgctcatag acttcgatct ttccaacaaa gaaaaaccat tcattgttga tctgggaaat
    49921 ttggtagcct actgcctttt cttctttgat tttcaaacga cgaggacgat aatgccataa
    49981 catcgtttcc cgaatgtaag tatctaaaac agcggttggt aactttttgc tccgtgtcaa
    50041 atcatactga taaaatattt cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa
    50101 catcattgtt tcgtatacat tcagttctgc tgctgttcga ctaagatgca tcacgatgat
    50161 tggatcgatt gcttgataaa gccggtccga ctgatagagt aatttctttt ctttcaaagt
    50221 tgcgtacgta cttttttcat tcaatagtct ttttttcgta atcccgagcc agcaagcaat
    50281 ctgtgcttct gctgatgccg taaacgttgt ttctaataca cggtccaatc ctagaatgaa
    50341 cggtcttttt tctggtgtat tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg
    50401 gaatagttca taataaaaat atcgtatctg tagctcttct ccgtataatt gaccgttttt
    50461 gatttgtaat ccaaattcct ctaagagctg gttcaattct ttgatctttc gaaaaacaga
    50521 agactcactg atattaaacg ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa
    50581 taaatagacg agcatcttaa acttcaagct ttcctgcaat aaaaacaata gaattttttc
    50641 caaacttaca tcttcgtcca acacaagcag cgcctgatac tcatttcgct gtacttctac
    50701 ttttttttcc atcattcggc caatcaattc aatatcttcc aaatattgat caaacgaggc
    50761 ttttgacagt tggacatgct ctctcaaacc attgatagag gctgttcccc cttgcagaac
    50821 aaggtttcgc aaaatcagga tctgtaactg ctcttttctt tctaataatg cttcaatctt
    50881 cataggcagt attcgccacc tttgaccaca ccatatctgg cacacggtat tctggctgga
    50941 aaaatagctc atgttcttct aactgttcaa acgtccatcc ttcttctact gcaattgtca
    51001 gttggtcaag aaggtggaac atctcttttt tgctttggag ctgtgctcca agaatctttt
    51061 tttcagaatc aaatagaaat ttgagtttaa ctttgctttg atttattgct gtaagcttgt
    51121 agtctacgta tcctgtttca attttttggg gataaaaaat gctctcttct tctgtcaaac
    51181 ctacgcttcc taagaaatag ccaaaccact gattgccgac tggacgatag gttccctgat
    51241 cctttgtctg tgagccacta attgtcttgc tgacatttcg tgctgtacgg acagcgttat
    51301 ttactagaga aacatacatt ttttcatttg ttggacgaaa agttacttgg atcgcatcgc
    51361 caatcgcata aacgtctttt tgagacgttt gcaagtagtc atctacccag atcgttccat
    51421 catcattcaa tgttaattgt tcttcccaca tctgattgtc ggggcgagaa tgattagcca
    51481 gcaaaacaag atcatagttc tcctttcttt tttccgtctc tacaatagcg gtttcatttt
    51541 cggtcaaaga cacaacttgt tcttctaaaa aaagtgaaag cccttgattt ttcatttcct
    51601 ttactaacgg ttctgtcatt tcaggatcta gataacgaaa aagaattgac tttcggcttt
    51661 cgtaaagatg gatttgtttt tttcttgatg ccaacccttc agctatttcg attcctgctt
    51721 gtcctgcacc aataattgca attttctttg cattcatgat cttttgctga agagccgtta
    51781 cgttttgtat ctttttaact gggtgaataa gcgttgattc tgcagaaata tttcgaaact
    51841 tttgatttga accagtcgct agaatcaaac gatcaaaatc caattttttt cctcctgcta
    51901 aaacgacttt tttttctttc aatcctatta ctgtcttttc tgtgtaaaca tggattcgat
    51961 agcggtcagt cagttcttct ttcgtgatcc aagaaagttg tttttcactg gtaacctttc
    52021 cttttaatat caaattgaac ccgctgggaa tccatcctaa cttcgattgc ttttcaatca
    52081 aatatatttc acttgttggc atcaattggc gaagataaat agccgcatga atccctgcaa
    52141 acgacccgcc gacaattaca atgttcatct gcattcctct ttcttataag gtgttctact
    52201 gctaacttct caatcagaaa acacagtatt cataactcgc tttcaacctt atgatacata
    52261 gcgatgcagc agaatcaacc ctctccatca taccgtgttc taggtctgct tctcaataat
    52321 gtactagtgg acctaattga agagtatcat ttctgaagag atgtttcaaa aaatgtactc
    52381 ttataaaaga aaaaaactcg ccttttacgt acctctgtcc aaataggaaa cagaaaagaa
    52441 cgaaaagacg agttttagcg tgtgtaatgt aatagttaga atagacacat catttatttt
    52501 taattcttta atgcttcaac aactgcttcg cctaattgat gagcagattg aggattttgc
    52561 cctgtaagaa gccgcccatc aatttgaacc acttctgaaa aagctgctcc agactgatag
    52621 tttgctcctt tttctttcaa agcatcttct gctaagaatg gtacttcatt cgttgtacca
    52681 tttgcttcct cttcttcatt actgaaccca gtcactgtac gtccagctaa aatagatttt
    52741 ccagactcat ctgtaactgg caacaatcct acaacaccgt gacaaacagc tgagaccagc
    52801 ccttcatttc gatagatttg tgaagcgact ttcccaagat ctattgcttc tggaaaatcc
    52861 cacattgttc cgtggccacc agcataataa accgctgcat aatcatttgc atccacatca
    52921 tcaggacgta aggtatttcc caaggcccga tttctgaaat cttcatcttc ataataggcc
    52981 caatcgattt catccatatt caaaacactt tgcggttcta aaggtacata tccgccttta
    53041 ggactgacaa aatctgcatc gatcccattt tttactaata catcataaaa atgagtcagt
    53101 tcacttaacc acaaacctgt aggacgctcg atcttttcgt acttttctac atttgttaat
    53161 acgatcaata ctttcatttt cccatcctct ttctataaaa attgcgttat tgataacgtg
    53221 ctttcaagaa attatataat gggcttggca aataaagctc tttatatgct tcttcaaaag
    53281 gtttcgttac aaattcatcc tcacggtttt ccaataattt ctctgcccag tcaggatcga
    53341 cgatcatttg ctgtccaatt gctgcaagtt ctgcatattt caatacctgc tctacatctt
    53401 cgcgattgcg gatcccaccg acaccaacta aagggatctt tccttgaatc gtgttatgga
    53461 ccacttcaag aatcgattta tcgttgtatt tatcagaacg agctattcga tcatacatat
    53521 ttaatgaaac atgcagataa tctagttttg actcacgtaa tcgttcaaga agccagatcg
    53581 tgtcttcaaa acggatacct ggtgtttcat attcttctgg tgagaaacga taccctacga
    53641 tgaatggttt tttagcatat tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa
    53701 taaaacgata acgattttcc aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg
    53761 gagagaaaaa ttgttggatc aaatacgtat ttgctccatg caactcgatt ccatcaaatc
    53821 ctgattcaat cgctctacgt gtggctttgc caaatgcttg gattgtttca acgatttctt
    53881 cttcactcat tgctcgtggt gtttccgcat caggacgttt tgctgcaact gcacttgcag
    53941 atacaggctg ttctccagat aaagttgctc gacttgtcat tcgtccaccg tggaaaatct
    54001 gaataatcgc ttttgcgcct tctccttgga ttgctttcgc tagctcactt aatcgaggaa
    54061 tcatttcatc atgagcgatg ctgagttcac ctggccagcc ttttccgata tcttggacat
    54121 ttgcagcacc agtaataaca gcaccaagac ctttagaacg ttgtttataa taagtgatct
    54181 catcttcggt gatcactcca ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca
    54241 atcggttctt caattgaact cctgaaggta aagtaattga ttgatcaaat tgtgacattg
    54301 tcattcctcc cataattcgc tctgattaac ctatcgagaa tcatctatca ttaaaccact
    54361 cgattagttg tacatgcaac taatttgctt aaaaaatttt attttttttg taacttatct
    54421 gttgcatcat tcattgtctg ggctaattct ttacttgctt catttcccaa aactgctttg
    54481 tatgactgat gaagttcttc ccagctacca tcgacttcct tagcaaattg gcgtccttgt
    54541 tctgtaagat aaactaatga caagcgacct tcgctaactg ttttgacgta tcctttatat
    54601 tctaattttt caacaaaacg agcaatcgtt gatggggcgg aatagagcat ttgtgcaata
    54661 tctttttgtg gtattccatc tttttctttt aatatcaata aaataaaacc ataagttggt
    54721 gccagacctg tccttctaaa cgcttcatca gctaatttat tcaacatgcg atctagcttt
    54781 ttcacgctaa agaaaagaca ttcatctaac gctttttcaa ttggcttttc catttcatcc
    54841 cctccattgg cgtacatatc atagtatgat ttttattttt tgtcaaaaaa cacggaataa
    54901 tgtccgcctc ctctgtctag atttgaaaaa aagaagtagc catcttcaaa gaatttcggc
    54961 ttgttttcga caaaactcct tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt
    55021 agttttgagt atgaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg
    55081 gcttaatacc gaaaaactgg cttttgaaca ccgtttatat cagaggttgt gaaaaagccg
    55141 tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt tgtcgttttt
    55201 tgagctaatg ccgatggtca gctgattgta caccgtttat tgagaggtcg tgagcctgcc
    55261 gttgaactcc gagtcacaag gagcaatttc aaaaaacagc ttctcatgtt ttatgactat
    55321 ggtgacttgt gatcgaggag ttggcaggag aatactgttt attcgtaaag aaggggctgt
    55381 gacaagactt ttgtcacagt cccttatttc cttttattat aggatttcgc catttgattg
    55441 gatgacttgt ctgtaccaat caaatgattt tttctttaac cgtttctttg tgccattccc
    55501 ttcgtcatct tgatcaacat agatatatcc ataacgtttt gacatttgtg aagtagaagc
    55561 gctgacgata tcaatgcagc cccacgacgt ataaccaaat aaatctactc catctttgat
    55621 agcttccttc atctgctcaa tatgctgttt caaatagtca atgcggtaat cgtcttgaac
    55681 ggttccatct tcttctactt tgtctatagc tccgagtccg ttttcagcta tgaacagtgg
    55741 tttttgatac ctatcatata attgattcaa agcaattctc aaacccttag gatcaatctg
    55801 ccagccccat tcacttgatg gaagatacgg atttttaatt cctccagtta ttaagtttcc
    55861 ttggacccct tcccaatttt ctggatgagc actgactgca ctagacatgt agtagctaaa
    55921 agaaacaaaa tcaacagggt aggctgctaa gatttcttca tcacctactt ctatttttaa
    55981 gttgacacct ttttcttcaa acattcgttg cgtatacgct ggataatatc ctcttgcttg
    56041 aacatcacta aagaacaaag aagttctttg ctgctcaatt gttttttgaa tatcatccgg
    56101 atcacaagaa tacggaacaa gaagctgata gctgatcata caaccgactt gtgaaccttt
    56161 gataatttca tgacaagctt ttgtagctaa agcacttgct ataaactgat ggtgtgcacc
    56221 ttggtatttc gcttgttcca aatggggatg gttttcttca attactccta cgctaacaaa
    56281 agggtgatgt tttacacagt tgatctcatt gaaagtcatc caatatttta ctttattttt
    56341 atagcgtttg aatactgttt cagcgaaacg aacgtaaaac tgaataagct ttcgattagg
    56401 ccatccacca tattctgtaa ccaaatataa cggcatttca taatgggaaa gagaaacgac
    56461 tggctcaatc ccatatttta gacattcgtc aaacacttga tcataaaaaa ataatcccgc
    56521 ttcattgggt gtttcttcat caccatgagg aaaaattctt gtccatgcga tagacatccg
    56581 atagcattta aaacccattt cagcaaataa acggatgtct tctttaaagt gatgatagaa
    56641 atcatttccg tgtcttttag gataataata cttgctgttg ggatcctgcg ccgcttttac
    56701 ttgctgatgc gtcatcatat gccattgatc tgtccatttt tctttaggta aatcgctgtc
    56761 gaatgtatac acatcagaca cagataatcc tttttcatct acatcccatg caccttcaca
    56821 ttgattggcc gctgtcgctc cgccccataa aaatccttct ggaaattttt tcatatcgat
    56881 ccccctaaac ttgctagttt tcttgtttga ttatttttct tatgactagt aatgatattt
    56941 ttgaatctac tactatttgt aacaataacc attgtggtcg gatcatatcc ctctttgatt
    57001 aattcttgca aatctacttc catcaataaa tcgcctttac gtactttttt tccatcagat
    57061 actgcacttt gaaagtattt gccatttaaa ttcactgtat caataccgat atggatcagt
    57121 atctctgctc cttcttcact gactacccca attgcatgtt tcgttggaaa aactgttgaa
    57181 acttccccgt ttacaggact gtagattttt ccgtccgttg gaatgatcgc aaacccttct
    57241 cctaaagatc cactagcaaa tgttggatcg tttacttcag ataaatcaat tatttcacct
    57301 gctgcaggag tacttaacac tatatccttt gaggattctt cttcgatttc atccttgtag
    57361 ccgaaaagaa aagttagcat acaggaactg atcatcgcta agaaataact agcaactagc
    57421 caaacaaatc cttcaccaat ataaatcggg aaagacaaaa tacttcttgt agcaactgcc
    57481 aaagcaccgg ctcctgcggc accagctact ccaccaaata tagctccaca tatacaagca
    57541 atgtaaaatg gtcgtttata tttaagagtc acaccataaa cagcaggttc agtgattcca
    57601 aataatgctg tgataaatgc agataaagat aaacttttta ctttcttatt tttcgttttc
    57661 aaaaataccc caagtactgc tcctgcttgc gcagctacgg cagtcataca tgctggacct
    57721 agcgtatcac gaccgtagaa agaaagattg ttggtcatta ctggtacaag tccccaatgt
    57781 aacccaaaaa tcactaacac ttgccaaaat ccgccgataa cagcaccaga taaaacaggg
    57841 ctaaagttat aaacagctaa atatccagct gcaatcagct gacttgcata atctgcaatt
    57901 ggacctacaa ctaataaagt aattgggacc atcacgatga tacaaatgaa tggtgtcaat
    57961 aaatttcgaa tactttcgtg caagcttttg cgaatcttag gctcaatata agaaagaacc
    58021 catacagcta aaattgctgg aataaccgta cttgtatacc ttgctagaat cacgggcatc
    58081 cctagaaatc gcaaggttat gctatcacta tatgcactga caatcgtagg ataaacgaga
    58141 gctgctgcaa taaccatagc gatgaaacga tctgtattga acttttttgc agcagtatat
    58201 gccaaaaata taggcaagaa atagaaaaat gcatcagctg ctgcattcaa aatgatataa
    58261 gttcctgatt cagtagttaa taaattcaaa ctcgtacata aggcgagtac tcctttgatc
    58321 aaaccacttc ctgccaagac gcctaaagtc ggggcaaaaa cagcggcgat catgtctata
    58381 aaagagttta aaatgccttt tttctcagtt cctctctttc cactagatgc atcatcttct
    58441 agtttcattt gtttggataa tacgtcatac acttcattta ctgcatttcc tactacgact
    58501 tgaaacattc ctccagcttc gactacagtg atcacacctt ctagttgctc taccttctcc
    58561 ttatctgcct tcttattatc ttttagggta aagcgtaacc ttgttgcaca atggtaaaca
    58621 ttgctgacat tttcttcgcc tccgacaaat cggacaatat ctttcgctaa ttgtgaatag
    58681 tccattttta cttccccctt gttaattaat gccgtagcaa tccttcttac aagataagta
    58741 tagttcttag aaaacggttt ctccattcaa ttagtacccc ttataatagc gaacgaaact
    58801 gtacaatagt gtgtatggtt tacttagccg agcaaatcac tcactgcttg aaagaaaaca
    58861 agtatatcgt ttctcttata gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc
    58921 tgatgtataa gtggtatctc aagctgatac tctgtgattt ttgtttctac ttggcttttg
    58981 ataagacagc acaacaaagt ctcggcattt aacggaagca taactaggtc agaactagga
    59041 gagattagtt gctgaatgga atctatcttt ttatggattt ttatttgatt ttcttctttg
    59101 tagtaatgat caagcagttt tgttttcttt ttttcttcaa ctatggtcag cttttcacgc
    59161 aaaatctgat aaatctccca agattcaaac tcatctagaa cactaggtag ataaatcaaa
    59221 tcatcccact caaaaataag ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt
    59281 ttgtttgatt gatttttgaa aagatacact ggttctatcc ggcattcttt gatccacatg
    59341 ttcatacttt gttgaatctg tttttttagt gtattcaaaa tactaattgg ctggttacta
    59401 acaatcagta tctccccttg ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca
    59461 aaataagcgc tttcagctaa attgggacgt aattgaaaat gtattgggaa gaaacgccga
    59521 attaaataaa tttctaaagg ataacgggag atgatctttt gtacaaaatg attcatggta
    59581 ttatgtccgg catccatcct cgttttcatt tgtttgatat gggttagcaa aaattctgtt
    59641 tcatggaaca ataacgaaac aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct
    59701 cttagattct tctctatttc tttgtttttt tcatcattaa tcatcaaata atttaactct
    59761 aatagtcgat tttcaattgc ttgtttttct tgattggaaa aagaataggg tagttctgat
    59821 gataactttt ttgtgagggt ctctaccatc ggaataaata atgtatacct gtttgttttt
    59881 tctaaaagtt cttctcttcg aagaatggtc aaaacgagat accgagaaaa tcgtatgaag
    59941 tcctctcctg aaacaatata attatatgat ataagaacct cttttaatgt cttttggaca
    60001 gtcggcatga tattggaaaa aattgtactg atttgttgag gcaaccttgc gtgttggatc
    60061 accgtttctg ttccaacaag agataagaaa attctttttg aattttcttc agcaatgatt
    60121 tttagtcctt ttgtcgacga aacttccagt tctaatttag gattgcgctc gatccaacgc
    60181 aaaatcgttt ttatttgtaa tgatacggct gtttttgaaa gaaagaaaat gtctgctatc
    60241 ttttgcatac tgacataccc tttttgaaac aataagtacg tcacaatagt tgaagctcgg
    60301 aaatccatgc tggaatagat tttatgtttc gtcacctctt gtatgatttg ctgtttagtt
    60361 tcagattcaa ttgattctaa aaaataacct gaacgtttat ttgactggat aatgattttc
    60421 ttctcgtaga tagcaagcat atttttgatt tcagtttgga tcgttttctt gttggtacct
    60481 aaagcttcag ctaattcttc gcttgttatc caatgattct tatttcttaa tactacaaga
    60541 atttggagtt gtcgtttatt taagtccatt tgcttacacc tcctctttgt tataaacata
    60601 caggaaaaac ctctcaaatg acaatagtca cttgcagagg tttaaaaatc atttgaagaa
    60661 agatctatat tcaaggtttt taccattaga ctggataatt tgtttataat attcaaaact
    60721 atcttttttc aatctcctaa agcttccgct cccatcattt tgtctatcca cataaataaa
    60781 gccatatcgc ttcctcattt cccctgttcc tgccgatacg atatcgattg gtccccacca
    60841 taagtaacca atgatttggc aaccatcttt tattgcctca gcaatttcct gaagatgtat
    60901 ttttagatac tcacaacgtt ttggatcgtg gattcgattc aattcatctg gtgcttcatc
    60961 tagtccaatg ccattttcaa ctatgaataa tggtaattca taacggtctg tcaattcatt
    61021 catgacataa cgaaacccta gaggatcaat tggccaactc caaggttcgg gagatttttc
    61081 cttcaaaaag ggattatcga aaccatttgc tccgccagta tctacgcgca tttctgcttc
    61141 ttttttatag acagcgctac gataataact aaatgcaata taatctgccg tgttagcttg
    61201 caagcactgt tcgtctccat ctaacattac tggtgcacag tcttcttcct tccatgtcct
    61261 gtaaatataa gctggataat gccctcggca catgacatcg gagaaaaacc atgaattgcg
    61321 tttaaattgc agtgaaccaa atacattctc tggatcacag tcatatgcat aagtcgctaa
    61381 tgcagaaaag ctacacataa tacccatctt agcatttgga tcgattttct ttaataattg
    61441 agtagcacgg gcgttcgcta gaaacatatg atgacaggct tgataaatca ctttttttgt
    61501 tagtgtagca atgggttcaa gtttatctct agcagggaat gtaaccaatc cagcagctgc
    61561 ataaggcata cgaaaagcat tattgatctc attaaaggtc atccaatatt tcactttccc
    61621 ttggtagcgt tcaaagacgg tctgcgcata aaattcaaaa aagtcgatta attttcgatt
    61681 ttgccacccc ccatactctc ctaccaatgc taagggggtt tcataatgac taagagtaat
    61741 tactggctcg atatcatatc gatttaatgt attgattagt ctgtcataaa atacgagacc
    61801 agcctcattt ggcttctgtt cgtctcctct tggaaatatt cgtgcccatg agatgcttgt
    61861 tcgaaaagct tttaaaccta actccttcag taaacgaata tcctcttcaa atcgatgata
    61921 aaaatcaatc gcttcatgag aaagataatg gagtgattcg tctaaagcaa tctcccaaat
    61981 ctttttttct tcattccact gaatactagg atgctggttc atgatcccga taagcgtatc
    62041 tgtcacatta ggtaatttgc cgtcttccaa ccatgcacct tcacattgat tagcagctac
    62101 tgctccgccc cataaaaaat tttctggaaa tatttcctga tccataacta tctctcctcg
    62161 ctatcaacaa tttcttactt gaagtatagc tcgattcatc aaaacaagat attctaacta
    62221 tttcggcaga agagaagaaa aaaattgaat gctactcttc atttttaata tttatatata
    62281 acaaatacat aagacgatcc ccgaaagaaa acactcgtta accatctaag caaacgattt
    62341 attttacttc caacacaaat gcttcataag gtcgtaaatc aactttgccg tctggtaact
    62401 gtttcagagg ataatttgac acaataaccc ttaaagcttc acgtggaaca gtgaacgtct
    62461 gcttttcact ggacatatta caaataacga gccattgctg gtcttcatat atgcgagaat
    62521 aggcaaagac atgctcaggc gtcttttcca ctaattcata gtctccccag acgatgatgt
    62581 catgttttct acgcaaatcg atcaatttct tgtacatata aaaaacagaa tcttgatctg
    62641 ctaacgcttc ttctgcattg atttctttat aatttggatt gactcgaagc catggaattc
    62701 cagtagtaaa cccagcatgt tcacgggcgt tccattgcat aggcgtccta gcattgtctc
    62761 ttcctttcgc attgattgat tccaagattt cctcttttgt aaatccagaa gaaatccttt
    62821 cgttatacat attgattgtc tcaatatctt ttgcttccgc gatatcatcg attgggcaat
    62881 tcgtcatgcc taactcttct ccttgataga tataaggggt gcctttcatc atatggagca
    62941 agatagcaaa catcttggca ctttcttttc gatacacatc gtcatttccc caacgagaaa
    63001 caattcgcgg aagatcgtga ttattccaaa ataaactatt ccatccttca tcacctaaag
    63061 atgtttgcca tttggataat acttttttca gttcagagat actcaagggt ttcaaatccc
    63121 atttgtcttt gccttcttgc tgatctaatc ctatgtgttc gaattggaag accatggata
    63181 attcatttct tttaggattg gaataaagtt tagcgatttc ggtagttgct ccccatgttt
    63241 cgccaacagt catcagattt ttgtcaccaa aagttgcttg attcatttcc tgaagatact
    63301 catgcagttt aggtccatta cttgtgattt ctttatccgg ttctttgcca atcatatcga
    63361 tcacgtccat acgaaagccc ccaatacctt tatcgatcca gaaattcatc atttgataaa
    63421 tctcttggcg aacctgttta tttttccaat tcaaatcagg ttgacgcttg ctatacaagt
    63481 gcagaaaata ttgccccgtt ttttcatcca actgccaagc agaaccactg aatattgagc
    63541 gcaatctatt aggctctttt ccattgaccg gatcacgcca aatataatag tcccgataag
    63601 gattttcttt atttttacgg gcctcaatga accacggatg ttcatcagaa gtatgattca
    63661 ccaccagatc catgatgata cggatatttc ttttctttgc ttcttcaatc agttcttcca
    63721 tttcttccat cgtaccgaat tctgccatga ttgcttgata attactgata tcatacccat
    63781 tgtcatcatt cggagactcg tatactggag aaagccaaat tgcatcaata cctaaatacg
    63841 ccagataatc caacctggaa ataatccccc gcaaatctcc aataccatct tgatttgaat
    63901 cttgaaaact tcttggatag atttgataaa caactgaatg gtgccaccat ttttgttcca
    63961 tgattttcct cctataaatg gatgtttcta ttttttgctt aatcatttat tttagtctct
    64021 ctcactaaaa attgtaacgc tttcaaatag ctattgcacc ttgttatcgg taacagaata
    64081 aaacaaaaaa acagctgagg aaattccctc aactgttttt tgaaaggatt gatttatgca
    64141 gggaagaatg ctgatttcac aacttcttta taaacggtgt tcactcagct gtcccttggt
    64201 attagctcaa aaaacggcaa aatatgagga actatttttt cgtttttttc tctaatactc
    64261 aggccagaac actgatttca caacctcttt ataaacggtg ttcactcagc tgtccctcgg
    64321 tattagctca aaaaacggca aaatataaga aactattttt tcgttttttt ctctaatact
    64381 caggccagaa cgctgatttc acaacctctt tatttcacat tcaatatcca catgaatccc
    64441 acaaatacaa agaacagacc atacatgatt ggagatactt cttttcctct tttagctgct
    64501 aacatggtaa ttggatagaa gataaaacct aatgcaatcc catctgaaat actataagtc
    64561 aaaggcatgc cgatcaagat caagaatgaa gggatcgcaa tctctaagtt tccccattcg
    64621 atttgtcgca aagattgtgc catcaatact cctaccataa tcaatgctgg tgcagttact
    64681 tgtgaagtaa caactgatag taatggagaa aagaacaagc ctaacaagaa gaaaatccct
    64741 gtagtaatag ctgtgatacc tgaacgtcct ccaacggcga taccagctga tgattctaca
    64801 aatgcaccaa caggagaagt accaaataag gaacccgcaa gcatagctgt tgaatcagaa
    64861 gctaaagctt tccctacacg aggcatcttg ttgtctttca tgaaacctgc ttggtttgct
    64921 aatccaacta aagttcctgc tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg
    64981 acccacattt gaagagaatt gatgtcccct acatgtttta atgcaactaa aaatgttggt
    65041 ttcaaacttg gtgctgcggc aacaatttca gaaggcattg gaatcaaacc tgtgaccaga
    65101 cctaaaatcg ttgtagctgc tatcccgatg aagatgccac ctggtacacg ccgaacaagc
    65161 aagatagcag ttacgactaa tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat
    65221 cctaatccta ctaatgtaga ttcattcgaa acgatgatcc caccttcgct cagaccaagg
    65281 aaagcgataa atagaccgat accgccagaa atcgcatatt tcaaatctgc tgggatcgca
    65341 tcaatgatca tttcccgcag cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg
    65401 aatacgccgg ctaatgctgt ttcccatgga atccccattc ctaaacaaac agaataagca
    65461 aaaaaagcat tgatcccaag agcaggtgcc gttccaattg gataacgggc taataccccc
    65521 attaaaacac agccgatcgc actggctaat gcagtcgcag taaacactgc tccttcatcc
    65581 attccagaag cacctaatac agttggattt acaaataaga tataagccat tgagataaat
    65641 gttgtaaatc ctgccagtac ctcacgtttt accgtggtgt tcaatttttc tatttcgaag
    65701 taggaaagta ttttttcctt catcttccat ccccctaaat ccgaacatta taaataaagt
    65761 ccgttaaaat ttcgatttat taataaaaca acgtgtttta tcatatacta aaacaaaaca
    65821 taagtaaaca aaaaattcgg attttaagca aaatccgaac gttccatctg attaaatccc
    65881 tttatctcaa agttcatgaa cattcacttt ggattacaag aatttcttta ttgtgttaat
    65941 aaagtttctt cattctagtc tcaaaaatca atagtgtgat ttgattttac catttcgttt
    66001 ctgttcttct ttgatgaatt cttttgtatc gacaggattc agattattta gtccgccaaa
    66061 acctgtatcg accatttttc cccagccttt tgtcttcttt tttcctgatt tgagattctt
    66121 tgctcgaagc ttctcactat gttttgctcg ccgatacttc aaataacctt ctaatcttcc
    66181 cattagatcc actgtttttc ctccttttta ttcaatcaga cgactcagct tttcctataa
    66241 caaaataatt gccttcaggg tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga
    66301 tttctcctgg ttcttccatg ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta
    66361 aagaaggcgg tggtcctaat acctctggtg aaaacttctg cacaaatact tttgggaata
    66421 cgcctaattc taattctttt gagatgctga gtacaatact cttaaattct tctggcaatt
    66481 cgattgtttc agaaatttct gcaccaattt tttctacaaa gaatcggcaa atcatctcta
    66541 cgtcattgac ataaagcatc acacgtgttt tttgtatcat catttgaccc ttctttctat
    66601 ttgattttct ttcctgtcta taaactgatt ccattatatc acgaaagcgc aaacaccttg
    66661 tgtttgatac tattcaagaa aaataaagca atagtttcta ggattgcttt cctccacaaa
    66721 ctttttttac aaaaaaaaaa aacggcaatg aatgccgctc ttcttatttc gattctttta
    66781 taattctgct tctgattcat ctttggttag tgtgtgtcta acactagtta cgccaaatgt
    66841 ttgatcgatt ttttccttca acgcttcgat tgcctgtgcg ccacgtgccg cttgttcagc
    66901 attgatggat tcaatgacca gcaccgctgc ttgtgtttct tccgtcagca ttgttctttg
    66961 ggcttcacgc cagttgagac agcgacagat cgctccttgt tcatcatagt agatgatttc
    67021 ttccggaagt gccggagcat cggaatctgc acctaaaggc aggaatgatt cgccaccttg
    67081 ggcttttcct aaatgcagat ttcctgcgat cttttcgata tcttctccgc cgcaaggtac
    67141 gccaaaagac aaggaaatac tattatagat atctacaagc ggattgattg ggaaaaattc
    67201 ccgttcttga tttgcacgtt tcagtaaagc ttcaatagaa gaacgtgcac cttttttagt
    67261 cttgaatgtg gtaaatgcct gacgccactg agcaatgact tcgttttggc tgaatgtttc
    67321 ttctgtaagg taatttcttg cttcttttgc tgcctcattc aataattgat gatacgtttc
    67381 ttcactttct tttgttacgt ggttatcgat accatttacg gataagattt cgatggttgc
    67441 ttcaggaaat acttcccaga aattggtatc cactattact tttttcatct gttcacacct
    67501 cttgattaga ataattgctt gatgaataga tagtctgttt ggggatcatc tcccatataa
    67561 aaaacatgtt cgctgtattt gataaatcct tttttacgat aaaaagcgag cgcaacatcg
    67621 ttgtgttccc aaacaccgag ccaaatggct gttttttgct tttcttttgc tttttcaata
    67681 gctgtatcta gtaaaaatgt accgatacct tgatgtttaa atttttttcg tacatagatt
    67741 cgctcgattt ctaaaaaatg atcagccatt tcctcagatt gactcgttcc tgtatttagt
    67801 ttcaggtaac ccgcaggctc ttgatcgata ctcactagga aaaaatcggt ttctttatct
    67861 tttagctctt ctgtaagttt ttctagtgca taagcttgtg tcaagtaatc agctaaatgc
    67921 tccggtgaat tatcttttcc aaatgtatct gtaaatgttt cgatactaat ttgttgtagt
    67981 aagtctacat ctgaaggcgt catttttcga atttcgtatt tattcacggc ttcttccttt
    68041 caatagatcc ttttattgcc ttttttgaca aaattccaat cttcgctaac attcttgcat
    68101 actttattta aaagatattc caattgtttt gcttcttcgt cagaaagacc atttaatgcg
    68161 actgcattgg agtagttgtt ttctcgaatg atgaaaggat agatttctgc tcctttttta
    68221 gtcggataga gtttcttgat ttttttattt tctttatctt ccaaacgttc gatcatccca
    68281 ttggattcta atttcttgat tgctcgggca gtcgttgtac gatctacttt gatcatttct
    68341 gctagttttt cttggatgat tccaggattt tcgcaaatac gtactaaata aagatactgt
    68401 cctcttgtta aatcgacttc tttgaattcg atattgctaa tagaatctag cgctcgggca
    68461 atcattccga tgtctcgaag gatttcagcc atttcatccc acctttattt tgtttgtaag
    68521 actagtttac ctctttttgt tgcaaatgca actattttca ataaaaaaat caagttcttt
    68581 tttaataact agtaaagtat attcttttct tcttttacga aaaagctcaa tcacaataga
    68641 tctggttttt gttttctaaa ataagtaact gtttcttcgt tgttcgcttt tctgatagct
    68701 ttggcaactc ttctatacta aaaaatccca tgttggaagt ttcattattc tcaataaact
    68761 tggcctcttc accgtggatc gcacaagcaa aaacaagttt ataatactga aaaagctgtg
    68821 gaatatcttt ttgtttattc gtatcaaaaa ctgctcgcaa ctgagtagtt tctactgtta
    68881 atcctgtttc ttcatagact tctttacgca cattttcttc aggagacaat cctatttcag
    68941 caaatccacc aggaagtgac cattctttcg tccgtttatc ttcgattagt aggaccttgc
    69001 catctttttt gatataggca cgaacatcta cttttggtgt tggatgtcct tctccaatcg
    69061 ttgtgagcgc ttttatttct tcgaaagttt cttttcctat ttcactgatc agttccaatg
    69121 aaatcgtccg gatctcctca aaacgctctt tatcaaatac atcttttcca taatatagac
    69181 cagcttgaga aagtgcgagt agtcttttgt attgagccaa cttcttgttc attctcttcc
    69241 cttctttttt agcaaatgta tgactatgta atcaagtata gaagactggt ttaattacgg
    69301 caaataaata taatgaaatc tgcaataaat aaaagaatgt taaaaaactc gagaacaaaa
    69361 gacattcgac ttctctgggg ctgtgaataa atggcgttca aaagcttgca tctgcggtaa
    69421 taagccccac caaacgaaaa atatgacgag ctattcttgt ctggctgttc ttattaattg
    69481 aggcaggaca gctttttcac aacctcccca ctaaacggtg ttctcctacc aactcctcgg
    69541 tcacaagtca caatattcat caaacatgag aagctgtttt tttgaaattg ctccttgtga
    69601 ctcggagctg aacgacaggc tcacgacctc tcggtaaaca gcgttcaaaa gccaattttt
    69661 cgatattaag ccatttcttt gttacttatt gtcattgatt tctttttcta atttatccaa
    69721 tagtggagtg taatccactt gagtatagtc tgttacatca aattcatata gtgttcctaa
    69781 ctggaatttg tcatattttc ggtcggcgat gatctttttg aatgcttctt tcgtaatatg
    69841 attggtccct ttcgttcgat cttccaatac gtcaccatag tgataatgat ccatgatata
    69901 gctcaaatga cgatctgatt cacgatcacg tttgtaccga cgttgacata acacttcaaa
    69961 gtctgctgtt aggcgaatcg tgatcacctg atatccgtat ctatctgcta attcttggag
    70021 tcgtgctttt tgcttatcac taaaaggata ctcggacaca atcactttct ttcctgcttc
    70081 catatacaaa tcaagtgctt tgtaatagaa aggataaact ttcgtccttt cttgttccgc
    70141 ccgttcttcc aaattgttaa acccgatgga ttctgcatag tattccttga tttcatcgag
    70201 cgtgatggca tacatatctg gaaatcgttc ccgtagttta ttcataagat atgtttttcc
    70261 tgtaccagga ctaccagcca gaagaatgaa atactttttc atctcattta tttcctaagt
    70321 taattggact ttccaagttc ataaacagtc caatcttatg cttgacaatt tcttttactg
    70381 cttcatttgc tggaatcaca ttatggcgaa gagacttgtt gatttctgtt tcactaaagg
    70441 tttctttagc tactttcgtc cagttgacta aaagctccgt tccaacattg aatttgcgga
    70501 tcccgttatt gatcaattca ggataatctt cttctttcac acctgttcct ccatggataa
    70561 ctagggggac atcaactact gcatcgattt ctttcagcaa gggaatgttt acttctgttt
    70621 ttgatttgaa ttgtccatga ttcgtaccga tagcaatagc taatgcatcc acgcctgtag
    70681 cttctacaaa tttcactgcg tcttccggtc ttgtatatac cttgtcattt tcagcgacat
    70741 ggattccttc ttctgtaccg ccgattgttc caagttctcc ttctacggaa acaccgtgcg
    70801 catgggcata ttctactact gcttttgtct tcaggatatt ttctttgaaa ggcagatggg
    70861 acccatcata catgacagaa gtatatccgc tgtcgattgc ttctttgata tcatcaaagt
    70921 ctcgggcgtg gtctaagtgc aagactgcat caaccatttc ttcttctgca atagctgtca
    70981 ttacattgac taacacatgc atgccgatat atttagcggt atcgacactt gtttgaataa
    71041 tgatcggcga acccatttct tttgctgcgc ctagcatagc aggaagcatt tctaggttat
    71101 gcgtattaaa agctccaact gtaaaattca actcttctgc tgtttttgtt acttctttca
    71161 atgttgtata catcttttat tgccacctta tctttttatt tgtttccttt gatcgttact
    71221 tgttttgcaa tttgtcgcat ctcatccatt ttgcccatgt agtaatcgat cccttgatca
    71281 tcaccagcac gagcagcatc tgcacgcttc tggttataga ggctcatcag ttttttatac
    71341 ccatctccga tcgtctgttt attttccaaa ctcttttcta aatattggat ggcttgatcg
    71401 ttcacataca attcagaaaa tttaagtcca acttgttcat aaagtttcgc taatttatgc
    71461 ttatcttggg tagtggaaat ctcttgtttc aattgtgtaa tctctgattg aatcttttcg
    71521 gtcgcttctg gcgatagggc tttttcttct tttgccagaa cttttttcgg ttctttttct
    71581 tttttcttaa aaaatccaaa catcattttc cctactttca attaaaatgc tttcaaaact
    71641 gggcttaaga tccaggcata taagagtgct gcggctactg tatctacatc ggttgctgta
    71701 gcattgatga atcccatcga attaaagatc gttactagca aagcgggcaa taatgtaata
    71761 aagaaaccat gagcaatacc gccaatgatc gctcctcggc gtccgccaac tgcgttgcca
    71821 aatatccctg ctgtccctcc ggcaaagaaa ttggttagca taccaggtaa aatcatcgct
    71881 aagccgaaca tgggcaaagt aaacatggca ataatcgtac caatcgttgt cgtaatgaaa
    71941 cctaaaatga cagcatttgg gctataagga aagaaaactg gacaatctaa ggcagggatc
    72001 gcatcaggaa ctaatttcat agctatccct cgaaatgccg gcacgatttc acctaacagt
    72061 agacgtacac cagctagcaa tacatacaca cccactacga attggatggc ttgtaaaaag
    72121 gcaaacatga tatagttttg atctcctgat aattcagaag cgaatggttc gcctgcaaat
    72181 aaaacagtga tgatatatag cgggaccatc acgaccatga cagacaaata agtatcttgc
    72241 agaaattcga aatgtgtcgg caacttgata tcttcgatcg actttttatt ttcgcctttc
    72301 tcaccaaaaa gcttagcaac accggcttca aataagtacc cgatcgtaca aaaatgacct
    72361 aaagcaatat catttgatcc agtgatttta cgaatgatcg gttgcgctac tgccggcata
    72421 gcaatggcga aacaagctcc gataaaacca ccgaccaaga tcaatacgat ccctcgcaga
    72481 ccggcaaaat agccaaagac tgtcgtcatc gttgccatcc ataaaattgc ttgaccagtt
    72541 aaaaaaatgt acttccattt tgtgaaacga gccaaaataa tattgaatac aaaaatggct
    72601 aagaacgtca gtgcaatatc tctacctaaa cccaaatcat tcattgcttg accattgata
    72661 gattcaatag aagggatgat cccttgcata tgaaatcctt cagtaaagat tttcccgaaa
    72721 taagttaaac ttccaacgat gatgctcgaa ccggcactca acacttgaaa tcctaggagt
    72781 gtctttaacg tgccagagat gacttgtcct gctgatttct tctgcagtag tagacccaac
    72841 atagcaatca atgcaatggt gatcgatgcc tgagtcaaaa tattttcgat gataaaattg
    72901 ataacgccca tgtttttacc ctcttttctt tttgtattta gataactccc ttatcttgca
    72961 gaactggggt gattttttct tcaatttcat tttttgaaac aatatttttc aaatagatca
    73021 tcgccgtttt aggatcaact gcgaactttt cgaactgcga acgaaaattt tctgctgtga
    73081 tgatcacatc tggtttcatg gaaactgcgg aagaaatatc acaatggtcc agtttggctt
    73141 ctactcctaa tttgttcaaa acatcctcag cagacatctg tgctgcaaaa ctacttccta
    73201 aacctgcacc gcatacgaat aaaatgttca atttgttcat ctttgttctc ccccgtatca
    73261 tttattattt ctcaactact gaaaaaagca gttgtttgaa atcttcgata tttgtgcatt
    73321 caatcaatcg atccaacttc tcttcgttgt tgatcaattc aattagttct ttcatgatgt
    73381 tcagatgcga gtaggagtct actgctgcca aacaaaaaat aatctttact ggatccattt
    73441 caggattacc gaaatcgatt ggttgctcaa tcgtagctac gctgactcct aatttattga
    73501 ccccatcttc tggtcgggca tgggctagtg ccatgtgttt gccaatcacg atatacggtc
    73561 cgtaatcatt cactgcacga atcatcgctt cgacatagcg ctcttcgaca atgttttctt
    73621 taaccaatgg tctagcagct agttcaatcg attcctccca agtagtggct ttttctttga
    73681 tcatgatatt gctgtccgtc aaaatatctt ttaacatagg ctggatctcc cttttgttga
    73741 tttctaaatt gtttgtttcg aataattttt ctaacttgcc atagatgatt cgatctactc
    73801 ggccaccact ttctttcatg acatctatta tttgagtgaa taactccgtc gattcatgac
    73861 tgttaggctc taaccgctgt agttcttcat ttcttttcaa aaagtcttct ataaccaaga
    73921 tattcgtctc agttaaaatc ggatcgacaa ctaaaagcgg cttatttcga taattcaatt
    73981 gaaaggtgct gaaaaccaga ttgacatcca atttatctac taattcgatt tcttttgaac
    74041 ttaaaactgc agtaatctcg atctgaggaa acttttcttt cagattctct gctaacagat
    74101 ttccagtagc catcccatgg ttacagacaa cgactgattt ataaacataa ttcaagtctc
    74161 gcttgattac actggcgatc gtcgaaaagt ggatgacaag aaaagctatc tcgtcctcca
    74221 gtatcttgcc accaattact tcttcgatcg taggaacaaa atgagtgact gcgcggtaga
    74281 ttgggaaata atttgttcgg atgttttctt tcaatggatt catgacttgg atatgattct
    74341 tgattcttac aatcagtgca gccatgtgct tatagagatt ttcgaataaa gcttcttcct
    74401 ttagatggaa aggtacgtgt gtctcgttct ctacaaattg gatcaattgg atcgttagta
    74461 attgagcttg tacccactcg attgaattgg agatatctct agcattgaac gtctcaatag
    74521 tgaaacgaag ataatttctt tctacttgcg ggacattgtc caattgaaat tcctcgatca
    74581 tgtggtcaat aaatgcagaa aagctactgt cttttatttc tgtatttttc caattaatgg
    74641 cagagataaa ttcatgtttc ctgattcgct ggatccagat caatgtaaaa agcaggattt
    74701 gttttttata aatatcatct tcttgtttca acaacttatt ggaatagatc tcctgtattt
    74761 ttttgatttc caccaaagga aaataatgga gaaagatttt ttgagccggt gttaattgtg
    74821 catcttgatt cataaagaag tcggtcattc ccagattctg attgataaaa tcatagatca
    74881 tcgtacggat agatcgttct gaacctttat aaaccagtcc ttgctttcca taactgacga
    74941 tctctatccc ataactgacc agtgttgctc gtaaccgtct catatcttca tctaatgtac
    75001 ttttagagat ttgaaattct tcttcttttt tgtttagatg gatcgggatt cggctgtaag
    75061 caacggctaa aactagatca aaaatacgtt cgcgccgatt caaaaagccg tctttcacat
    75121 cttttatttc agtttctaat cgagcttgct tttccttttc cagcttcaac aaaaaaactt
    75181 tgttgcgaat tgtacggatc tccggaagct ttttactgct caaccagata ttcgcttcaa
    75241 gaacttcctg acggatcgtc cgctgactga cattgaactc acttgctaga tgttttgctt
    75301 ttactggata ttgggctgtc atgattctgt gaagtatttc aattgttctt gttttcatag
    75361 aaatgatctc cttctgattt caagtatatg gttccgctta cataataaaa agtagatatt
    75421 tctgccactt gaagtggaaa gaaagtaagc aaagcaaaca aaaaataagc catatgcttt
    75481 ttcatctcct caacgaaaaa gacatacgcc tattattaca aaaattttgc ccatctatac
    75541 aaaaaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacaact aaactaaaat
    75601 agtgcttcct atttttcgtt tggttgttct tattatcgca gatgcaagct tttaaacacc
    75661 atttagtgga gaggttgtga cagagatgtt cagccttgag cgataagaag cgattttgaa
    75721 aaatcgcttc ccatatttta caaaatcgtg ccttattgcc gataggctat ctctggcaca
    75781 ctgtttattc agaaaaagga gctgcgacaa ggcttttgtc acaactccgt tcataaatat
    75841 ttcctgtcaa tccagcataa tcggctgaat aaaatcggct tgccatcttt tttctttctc
    75901 agaaaaatca aaataccata tctgagaaaa atactgtcgt tgtgtcttac ttcctgagat
    75961 caatcgccca tcttttcgat cgactgaata atcacagcat ctgaaatcaa ttcggatggc
    76021 aaaactattt tcgtggatct gtcgataatt tcctacttcc tgaatagtta tttttttagt
    76081 atgatttctt acaccgtctg ctttgttttt ttgcagaact ttctgatgtt cggcaaacag
    76141 cttgtctgta taacagcttc tggctttttc aggagtttct tcttcccaag cggattggat
    76201 cttcatgaat atcttttgga tttccagaaa ctgtgctttc ttttctttag ccgttcctga
    76261 catgcgggac aatatctccg ctttcagcct tttcttttgt cttttattct tgacgataga
    76321 aaaagcagaa acccctccta ctgacaaaac agctaatgca agaaatcccg tttgctcata
    76381 actagaagga cctgtacctg gaccataata cccataagaa tctctgccgt aagaacgact
    76441 gccaaaataa ctgcccccat atgaacgacc accaaatgaa cctctgcccg aacttcgacc
    76501 gctagatgta cctccaccat gaccacccgc gacagcgaat ccttgggtcg gcagagcaaa
    76561 caaacatagg cccactaaca aaatgattgc taaataattg atttttttca ttgtcttcct
    76621 cgttatcttt tattacaaaa aactattttt atgatagcct tgctaaaagg aagagtcaat
    76681 aaccagatgc ataaactgtt tttttagcaa ttttgtctta tttagtcatt gagtaacatc
    76741 ggacaaatgt atgtgtcggc agtccataat tcctctatct tcagcatgaa caagcttgca
    76801 gacaagagat ctgcgcttcc tccaggagag atttggtttt cctgacaaaa gtgcaacagt
    76861 gctgtaaatg gacgatttgg taaagcactt tgactagcag catgagcaag tttttgcatt
    76921 tccactaacg ttttctgatc actgcgatgc aatacgcatg tgtcatctag cgaactcatc
    76981 aaaactaaca acatatgcat ttgtttctgt acatatgaat cattatctag ctccttagct
    77041 gcttggattg caagcctgat atgcggatac cctaatatcg cttcttcata tgcaccattt
    77101 acgccatatt tttgcttcac cctatgacca tgagtggttt ttttatgacg atattttgta
    77161 tcaggaaaag cagcaagctt gctgacatca tcaagtattt tcgataaaga aaaaggctgt
    77221 tccatactga ttttttgtgc aatgacactg atcaatagcc ccattgccca aatcgctcct
    77281 ttatgggtat tgatgccatt tgtagcaatc agcattgcct tttccgcttt tcgcccaatc
    77341 tcagcaattc tttctcttaa ctcctgatca atagcatgat gccatgctat ttgagccatt
    77401 tcttcaaaaa aaggagttag cgtcttagca gacttaagaa ataaagcata gtccatatcg
    77461 gaatgtgcac ccgcatccag tgcatccact aatccaggtt ttggacttaa tgttacttct
    77521 gcgatcaatg cttcctctac caacgcagcg agttgctgag tcactgcttt ttttcgttcg
    77581 tcatttatca tgacactcac tccttcttcc tactattact tgctgaaacc ttttttgata
    77641 gctacccaaa tgatatgaag taaccagctt aaagcaaatg cacaacttgc cgtcattaga
    77701 taagctacaa gaatcatgac tcctgaaggt aactgcagcc aactatcttt aaacaataat
    77761 aacagtactt ggaaaacaaa tacattggct aaatacgtac gataagcata aacagacaac
    77821 catttgaaca atggcaattt tggcgaatcg tttacaatca ttttggaagc gcctatgaag
    77881 acagcaaaga tgatgactaa actgtataaa ctttggatcg tattcaagta aggtgcattc
    77941 gcaaagctca agtctcctgg atgtgcgagc aatgaacgga cagaaaagaa cgcgataact
    78001 aaggcaacag gtaagaaaag ataacgtact ctgtccaaat attgatagac tttttcgtga
    78061 taaacaaatg cggctacccc ataaatcatg aaactagcaa caaaccgatc taataagtac
    78121 cagctgtcat gataaggccc agtcaatact tgtgtttgat agaacagata ccatgcgaca
    78181 tataaaatcg ttgaaccaat caatactggc catacatatt ttttattagc aaatactttg
    78241 taaccgagcc aaacaaaaaa cggcatgaac aattggatct gcagcatcat aacggtatac
    78301 cataaatgag gcgctccatc acctgtgaca tatttcagta aaaagcttgc agtatccgta
    78361 tacggagttt gttgttggac tgctggaaat agccaaagat aagccgtcgt ccatagaaga
    78421 tacggaacga ctagttcaaa aaacttttct ttcaaaaatt cccaataaga caaatgctga
    78481 cttgttttta ccatattata aacaattgca aaaataaaca ttggtgctgt gtatttaacg
    78541 atgacgtaaa aggtaccaag aaaagctgta tttgttgcgc tgtggctgtt tgccaaagca
    78601 aagctcaata tcgtttgcat cataacagcc gttacagctg atactttctg gaagtcactt
    78661 acgttgattt tgattttttt gttttcaaac atctctttct ctctcttttt ctttattacc
    78721 aaagttcttt ggttaattta ggtccttcga ctgttttcaa tagaattttt ttctctggct
    78781 ctctcaaata ctcttttagt gaaaaaccgc cttgccgagt cactacttgg gtatcgaatt
    78841 gctcaaaaaa agcaggatgc gattgaatta cctgaattgg caattcaatc ggtgaatttg
    78901 cgtacagcag caaatcaaaa tcactggttt cttttactgc cggaatccca gtcgataatt
    78961 caaatcctaa gctgccgccg attccccatt tacatcctga taatatgttt ctagcagcat
    79021 gaaattgctg ataaacagga taatccatga ttttctcttg tctatacgat tcttgttcga
    79081 tgatgtccca cggatgaatg acttgtttga cggcatgttt tgagatttct gttgaccatc
    79141 tctgattgcg ggcaggtcca cgtaaaccaa tcggtagtat ttctgcgtga tccgtcgacg
    79201 cacgacgaac tgttccgaac atcttttcag ttagaacgac ccattcaggc aaggtgaacc
    79261 cttctatttc atgtttatca aaaaggacaa tatcatgtgc ttgaatcatt ttttatgcat
    79321 cccattcttc gtccattttg gcacggactt tatttgtttc gacacgtccg aatgttttgg
    79381 ctgtttcgtt tgtgtatcga tttgtcagca tcgtgtcatt tgtattgcgg acatcttgaa
    79441 tggcttcatt gattgcttcg atgactttgc tgacattttg ttctgaagca gcttggtcgg
    79501 tgatatcttc taggaaatga tagatcgctc ctagttttgt ataattattg atatcgtaag
    79561 cgatagctgg aactttttca gcagctgctt cgatttcagc aaccgaacgt tttgtcacac
    79621 gtgcagcact ttctttagac atcgcttgga cggtaatttc tttattgttt aaagcaatca
    79681 agcggcttga ttgtaatcca tgtgctaaaa atgcacctga aatagcattt cctgcaatca
    79741 atccaataac tggatgtcca gcttgacgga gttttgcata agcagcagca ctattagcca
    79801 aagccacatg gataccgatc aattcttcgt tatagccgta tgcttggctt ggtacatcga
    79861 tcacaacgat gattggccgt ttcacttttt tgtctttatc ttcttcgtaa acgtgatcca
    79921 aaatttgtcc catgcggaaa ccttcaacta aaccaacttc accgtttcgt acacggtaca
    79981 tcgggttttt aggatcaggt acgattgatg tcacgatggc ttcttctatg aatttacttg
    80041 atttaccata atacacagta ctaatagaag aagttggatt tttcatgcca gttagtgccg
    80101 caaaccaatc gtaaccaaca cttgctgcag ctggttcatt tccttctgct acttctggta
    80161 agtcaattga ttttgcttga tggttagcaa ttgcttcatt gtaatcgtca atggccattg
    80221 gttttgaaag atctaaagaa tcaagtaatg ataaataaaa tgctgagctt tcactgcgat
    80281 gcgtgtcttt tcgttcgtcc attgctgcga tgacagcagc tttgattgct tctacactat
    80341 cagtcactaa ttcatcaatg atttttgatt ctacccgttg acgagatcct aatgtattcc
    80401 aaatcaagtc tttgtcacta gaatcaaatt ctcgaacgcc tgcttcttgt tcaataactt
    80461 ctggtccatt caaaccgact ctcgcttttt ttgttgcgat taaataactc attaatgctg
    80521 aagtaatcga cataccgcca aatgatccta cacgtcctgg aatcaaaccg atgactggta
    80581 catattgttt tagagcaatg attacatttc cgatttctga aatagataac agtccgtagt
    80641 ttgcttcttg caaacgaaca ccacctgtat ctagtacaat gactggaaag atctcatttc
    80701 cttttttatt ttcttctaac acatgttcaa gtgcagcaac gatttttgca ccggaaactt
    80761 caccgatacc gccaccttgg aattttcctt ccatagcgat tactactgct tgtttgccat
    80821 tgatcgtgcc acggctgaca acgatcccgt catcactttc agggacgatt ccttgtgctg
    80881 gtaaacttgg cgcgatgatt tgatcgaatg gatcaatcaa ttcacgtcca ttgtcaagta
    80941 gagcgttcac tcgttctctg gcgtttaatt ctacaaaact atttttcatc tgaaagttcc
    81001 tccattgctt gcgtcaatcg taggtacaca actcctggtg tagcaccgaa gtcattgatc
    81061 acgatatctg ctgaaattgg ataacgatca aaaaatctcg ttaatacatt gttccatact
    81121 tcgccgaagc catcgcttcc tgtacaaatt tttacagtag attctgtttt ttctgttgga
    81181 aacatcaaga tttccaaatc tcctgaaccg accactccta catggatttt cttattgatc
    81241 ggctgtttcg ttggatattt ataagctaat tgttccatgg atctctcccc tttttgtaaa
    81301 acagagcata cacgttctgc ttattcgtct ttcttcttcc atctgggtaa atggcgacaa
    81361 aaggtttttc tcgccaattt tatgaccaag ttctgaattt cattggtggt tcatacaggc
    81421 catctgacca ttcgatcaaa tcatcgatgg tttgtgcagc taataatgaa cgtttcgctt
    81481 cactacgttt gatgcctaga tcttctggta atttcacgat gccggctgca cgcaagctat
    81541 ctagtgattc tttcgtgcta gtcattccaa gaggtgtaac accgccaatt gctgcaattg
    81601 ctgcttgtcg ttcttctttt gtttttgctt tgtacaaata agcaattcct tcttctgtca
    81661 cgatatgtgt agtatcttcg ctataaatca tcactggtgc attggctaag cctgcttgtt
    81721 tttgaacagc tagtgcatct aattcttcga caaagactgg ttttttattt gaaccatatg
    81781 tttcaaccat ttgaacaact aatttttggc ctttccctaa tggatcatca ttgtcgcgaa
    81841 gtgattgcca tgctggtgta gaatgacgac gaccgccagg attatgtccc atgttaggcg
    81901 caccgccaaa accagataaa cgtccttttg ttacggtaga agagtttcct tcgtaatcta
    81961 attgcaaggt tgaaccgata aacatatcga tggcatattg tcctgcagct tgtgataatg
    82021 ttcggttact gcgcatcgtg ccgtctttac cgacaaaaaa gacatctggg cgagcagcaa
    82081 tatatttttc cataccaact tcgccaccaa aactatggat gctttcaacc caacctgatt
    82141 cgatggctgg aatcatcgtt ggatgtgggt tcaataccca gttcttcgcg attttccctt
    82201 ttaaccctag tgattcacca taagttggca gtaacaattc gatagctgct gtattgaatc
    82261 caataccgtg attcaaagat tgtacgccgt gttctgcgta gattcctttg attgccatca
    82321 ttgccattag aatttgcaat tctgtgatgt tttgaggatc tcgtgtgaat aaagcttcta
    82381 attgataagg tttatctgct ttcacgacaa cgtctaccca gtcaccaggg atatctacac
    82441 gtggtaaaga atcgacaact tcgttgactt gaacgatcac gataccatct ttgaaagctg
    82501 cagcttcgat gattgttggt gtttcttccg tattgaaacc tgtgtacaag ttgccgtctt
    82561 tatctgcttt atctgcagct actaaaacaa catttggaat caaatcgatg aataagcgtc
    82621 cataaagttc caaataagta tggatatcac ctaatttcat tgtgccgtct tcaatcattt
    82681 gggcgacacg caagctttgt gcaccagcat aagagaaatc gatcttgctt gcgattcctt
    82741 tttcaaaaat attcaaatgt tctggtcgtg agatactaga catgatcaaa tgcagatcat
    82801 tcactttttc tgcatctact ttttcaagac tttctgaaag aaaacttgct tgtttttgat
    82861 tgtcgccttc taatacaact ttgtcgccgg gagcaatcag tttttccatt acttctacga
    82921 ttttttcagt ttctacatac ttgccattga gcaagttgct gattttttcc aatcgctgcg
    82981 cttttttttc gcgtttagaa gcccagttgc ggtttggttt ttccataatt agtcatctcc
    83041 atttttgata tagaaacgtg gcgtcatcaa taccatgttg gtctagattg atcgtacgga
    83101 tccctttatc tacaccaaat tttgtatgaa tcagctttgt caaagtattg ccggggggaa
    83161 attcaatcgt cagatccatt cctaattctt ttgccaagtc catcatttgg ttccactgca
    83221 cagggaaagc taggttttcc aacaaatctt tacgaatctc ttctgcgtcc tgagtggctc
    83281 ttccatccgt attttttata tacaaacatc tagcatcaga tagatggaca gactgcatat
    83341 acggcttgaa tttcttgacc gtatcattca tcaagagaca gtgagatggc acagggactt
    83401 tcagcagctt cgctgtttgc gcttggtgcc gtttggcttc tgccaacgta acggataaac
    83461 cttcgatact cccagataac gtatgttgca tcggacaatt ttcatttgac agataaactg
    83521 gatggtcagg atcaaatgtt tttgctacaa tttcctcagc ttcgcttctc gtcacaccta
    83581 ctactacgcc cattgcataa ccgcttggat aagcttcttt cattaagact gcgcggtgat
    83641 agactagttc cgctgcattc tcaaatctca aagatcgaca agcaacagct gcacaaaaag
    83701 caccaatcga atgtcctgct gcaatatcag gctgaatacc gtttttttcc atttcatcta
    83761 aataaaatac tgcttttgtc agtaatgcta gctggataaa aaccgtatct tgatagtttt
    83821 cttctttatc aactaaccga taccccgtca cattttctaa acgttccatg tacatttcag
    83881 ggatatccgc taacatccct aacttctgac tgccttgtcc aggaaataag aaacctgtac
    83941 tcatgagcct cgcctcctca gcacttactt tttccactgc tatcttagtc aatttctgct
    84001 ataataaaaa atgccaaaaa aagaaaaaac cataacaaaa aggtatggat gggatggata
    84061 attatgaaat tgcttcatct gcagtatttt aaaaaagtat tcgaaacaaa aaatgttacc
    84121 caagcagcca aagaattatt tatttctcaa cctgccttga gccgtgcgat caaacatttg
    84181 gaaaacgaat taggtgtccc tttgttttac catagcggca gaaacattga gacgaccgtt
    84241 tatgcagaag aattttatcc atatgcagtc aagacattag acacattaga agaaggaatc
    84301 aaattattgt cttcggtcaa tgagaagatc gttacttccg tcatattgta tttagaggta
    84361 gcctctgttt ctattccgaa cttggtccga atttttatgg aaaagcatcc tgatatccaa
    84421 ttaacgatca tgcaacatca tttaccagat gatattaccg atcctgtact gtacatcact
    84481 tcggagatga agcctggcat gaccaatatt cctatcatga aagaacctgt ttatgtcgcc
    84541 attcctaaga aacatcctct tgctaaaaaa gaacatctgc aattagaaga tatccaacgc
    84601 acccccatct tgatgctgtc aaagagcaac gccttccgtc atacactgga tgcagcactt
    84661 gaagcgaaaa gtatcgaatt gacgatcagt tcgacgacag atgatcctgc gacattacgt
    84721 tctattgtca agcaaggatt agctgttagc tttttcccaa aagtttcttg gtcttacgat
    84781 aaaaatgatc ctttcgtctt gcgggaaatc aaagatttgc ccttgacacg aacaatttat
    84841 ttatcttcta gcttcacaga agatcatcct ttgatcaaaa tgattgcaaa aacgatgcgg
    84901 gaattttatt taggaaaata aaaaaaaaag agctgggaca aaattcttgt cgcagctctt
    84961 ttttcgaaca aacggtgttc cagaagtaat ttcttagaaa ataagccgaa atttcccaaa
    85021 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctaaac atttctgtca
    85081 caacctcttc tctattgtcc tagacattca ttgcgttgat ttaaaacttc catcaatcaa
    85141 atctgctagt tcatctagct tttctatttt acgtagatca atagagatcc aatgttcttt
    85201 attcatgtga taggctggaa aatacccctc ttttcctcgc aaaattccgc caagttcagg
    85261 atcgaccttc aaatcaagaa tattgatgat cctatggtca cttgcatcat catagatttt
    85321 ttcttctgga acagacatca ggaccccaaa ccattttttg cttttttgat gtctgaatac
    85381 gcaataatca ggaaattttt taaaaatata ttctggagac aaattgtatg tttcattgat
    85441 gtgatcgatt agttcttttt tcgttagcat ctgcttccat ctctttctta ttgattcgct
    85501 ttattcaaag ataccacgta agaatagaaa aatgaataca agtagttgct aatcaaatac
    85561 ttcgatattt ttctttttta tcaactctta aatgttcaat ccgcctgtgt tggtcttgct
    85621 accatctact attaaaatgt ttattattgt ttgcccacct attttttgat tttagtggta
    85681 tcatttttca agttcatgat gaaattctta tcatcagtaa atgctccaga aaaataaaca
    85741 atgattgaaa aatcagttga aacttgacca ccgtttattt tctccgtatt cttagaatag
    85801 aagggagtct gattgatgta ttcactaatg aaaaaaatca ttaccgaaaa agatattcga
    85861 cgtcatgttt ctttagtaga acaattgctg aatcatacaa agataaccgt aaatgaatta
    85921 gctgaaatca tagggacaac cgaacgaacg attttttctg accttcaatc tattcgctct
    85981 cagctgcccg aaggctggga catcatcagc gatcaagcag gaatcagttt gcaaaaccag
    86041 caaaatttat taacaaatga cctttgggag atttttttca aacaatcagt cagtgtcgaa
    86101 ctgctgaaaa atctgctttt tacaaaaaaa gttgctgtcc ctgattttct agccgaccat
    86161 gggctttctt atgggacatt gaaaagacat gtgactaaaa tcaatcagcg tttagcaagt
    86221 tacgacttgc agatcgattt gacgaaatat acagcttgta ttcttggaaa agagcgtgcg
    86281 attcgtactt tttatcatcg attgctgatt cctttcactc ataacaacta ttttttcgag
    86341 gattattcga tccatgaatc ccattatttt caatttttaa gaaacctcag tcaaactgaa
    86401 ctagcggttg aaacagaaga gattttcgga acatgttggt tctttatcaa tacgatccgt
    86461 ataaaagcga attgccgatt agattcttct atccatacca acagcacact ctcctccctg
    86521 tatgactctg cattgaaaaa attgtattta acggaaggta tttatcttaa agacactgag
    86581 ctttcttttg cttctttctg ctttctggaa agttggaatt acaacaataa ctatggacaa
    86641 gaaaccgctc gctgcctgca tcattctcca tttttagagg tcctcgaaac gtttgtagag
    86701 gaactagctt cagagctgtc tcttgatcaa ctaaaaaaaa catctctgac tgataacttg
    86761 gctctcctca tattaaaata tcatgaatcg ccgatattga ttgaagcatt ggaccgtcaa
    86821 taccatcgtt ttttagaaac atatgatcag caatatgcac acttgtatca acagaaagag
    86881 aatttactga acgatctaaa aaaagaaata gaaatcaatg aaccaaatta ctttctccaa
    86941 ctcttgtctc tactgatcca acaaacgatt ttttctatca aaccgaattt gttcaatgtc
    87001 tattttattt tccaaggaga accttcatgg aaggcgttct tgcagcagga attaaaagat
    87061 tacttaggaa aacgtgtctg cttcttacct atcgaactaa ccgatctttc cactatttct
    87121 tttgaaaaaa cagatatctt gatttccaat ttccctctag attctctaga tatccctatt
    87181 gtatacattt cttctattcc aacgaaaaat gagctcaatc gtttgactga actcactttc
    87241 aaaacttttt tatagtaacg gctagcgact gtattctcta gctgtttttt cttttttgcg
    87301 aaacagtttg ttcatgatcc ctttttctat ttctacaact aagaatagaa gaatcacatt
    87361 tgctccaatc aataactgtt gtgtcatcgt taaacttact gtaccgatca gctgctggcc
    87421 gactggtaga aaaataacca tcccttgcaa taaagcaagg atacctaatg aaagaaaaag
    87481 cgctttgttt tgcaaaagac cttggttcaa tgacggcttg gacaactctc tgcaattgat
    87541 catgtaaaca gcttgagcta agacgatatt ttgcagaaga atggtttgct gtaagctttg
    87601 cccttcaaac tgcatagcca ataaataggc aggaaccatg atcagtaggg agacatagat
    87661 gatccgaaac aaatgataac cagataggat tccttgcttt ggatctcttg gtgggcgctt
    87721 catgatttct ggatctggcg actcgaaccc tagtgcataa gataaagtga ttgtcgttac
    87781 catattgacc cataaaatct gtactgggga caaaggtagc gggcgattca gcataagtgc
    87841 ccaaatcaag atcaatcctt gagctaatgc agttggcaag aagaaattga tggttttttg
    87901 caaattatcc aaaatccgcc ggccctcttt tacggctttt gcaatcgtat ggaaattatc
    87961 atctcctaaa accatatcag ctgcctgttt gcttacttca ctacctttga ttcccatagc
    88021 aattcctaca tcggatcttt tcaaagcagg tgcatcattt acgccgtctc cggtcattcc
    88081 gacgatttct ccatttttct gcaaagcttt aacaattcgc agtttatgtt ctggtgtcgt
    88141 tcgagcaaag acatcgacta ttggcacttg ctgaatcaac tcttcatcag acataagatc
    88201 caaatcgatc ccttctaaaa ctttggcagt atgttttaag ccaatttgtt ccgcaatcgc
    88261 ttttgctgtg tctttgtgat cccctgtaat cattttcact gagatccctg cttgttggca
    88321 ttcctttaca gctttgactg cactttcttt tggcggatcg atcaatccgg cgatccctgc
    88381 aaaagttaat tgggttaaag tctcatgcgt gagttcctgt ttcgaatcta cttttttata
    88441 agcaaatcct aaaacacgct gaccttttct cgcaaattct gcagctgtct gactccatgc
    88501 ttcttgttct gcagaagaaa gagttgattt agcaaataat acttctggtg cacctttgac
    88561 gtaaatcacc gctccttttt cctccgcatg catcgttgcc atatatttgt attcagaact
    88621 gaacggaatc tttgcttcta tttttttcaa aggtagattg gctgtttctg tgtgtcgaat
    88681 cagcgcaact tctgtcggat tgccacttaa attttctgtt ttctgttctt cttttgtttt
    88741 taattcctga cagtttttca taatggacaa cacgcaggct tcttcttttg cagctacatc
    88801 cacaactgtc atttcatttt ttgtcaatgt tcctgtctta tctgaacaga tcactgtcat
    88861 agaacctaat gtttctaccg aaggaagtcc tttgatgatc gccttttctt ttgccatttc
    88921 atgtactcca acggatagaa tcatggtcaa aacagctggc aatccttcgg gtaccattga
    88981 caccaccaaa gcaatcgtag ccgaaagaag gatatgccaa tccataccat atcgaaatgt
    89041 cgtaaaaata actaagaata ggctcaaaca aagaatgcct tgaaaaattt gtttgtttaa
    89101 tcggttgatt tttttaatca gaggcgtcgt ttgttgttcg accgattgca atgcttgatt
    89161 gattttaccg atttctgtcg tgtttcctgt ttctacaacg acaccgactg cagaaccaga
    89221 ttgtacaagc gttcctgaaa aagccatatt tttctggtcg ccgatcgatt cttcttctgt
    89281 caaaacggca tgactttttt cagatgcttc tgattctccc gtcaaaatcg attcttccac
    89341 taataaatca tgtgcttcga ttaatcggat atctgctgga acgacatctc cggcacgtaa
    89401 gacaatgaca tctcctttta ccagttcttt cgcagggatt ttttcttgca tcccgtttga
    89461 tagtactatc gcttcttgtc ccatcagctg cttcaaacca ttcaatgatt cttccgcttt
    89521 tctttcttgc cagtaactaa cgagtccatt gacgatcact acgaataaaa taatacttcc
    89581 ttcaatatat tcactagaag caaatttcaa aaaagaggca atgatcaaca cgatcatcag
    89641 cagatcagta aaatgctttg ctattttttt ccatcgtttt gtctgctgcc ttgcttttat
    89701 ttcattatat ccttgatcag tcaggcgttt ctgtcgttct tcttgcgaca agccttctgt
    89761 tgatgtttga tagtttttca atacatcttc taccgtcaat ctgtaccagt tcatcctatc
    89821 ccctcctgtt taattgatac tgttattata cgcagggaag ttttttcagg cgtatcgatt
    89881 atttcagggg ggtctgaaat aattcgtttt gcaaaatgta tctgggccta actgatattt
    89941 ctattgctta ttcttttata tacaaaattt aagaatacaa accagataac caataaaaaa
    90001 ggaataaagc gtgtcgtgct ttcgaataac aagctgaaag caattgaagt aaaaaacacc
    90061 aacaaagcaa gatctttata tctttgtgta gtgttccgtt tcttttcttt aataaattga
    90121 acatgtgcac acaagatcga agaccacaca aatagaaaac aactcgttgt catgcctgcc
    90181 aaaagctgaa aaaacaatcg actatcagct acaaaaaaat gtagacaaag accggcaaaa
    90241 aataacattc ctgtaattaa aataccgtgt aaaggtacgc ctttcttaga aagcgcacaa
    90301 aaccaatggg gcaaactttt ttcttttgct gtttgatata aaatcctgct agtactgtag
    90361 actgcactat ttgctgagga caaagaagca accaaaatca ttagattgac aattgctgct
    90421 ccgccttggc aatggatcac ttgcagcata gtcactaaag gactttcttt taaatctaaa
    90481 caatgccatg gaacaacaaa taaaatcgac aaaatcgttc ctatatagaa aaagagaata
    90541 cgccaaggca gacagttgat tgcttttttc aaattctttt gcggatcact tgtctcgcca
    90601 acggttaagc cgatcatttc aattccagca aaactgaata aaaccatagg aaatgagact
    90661 accattccag caaagccaaa cggtagtaat tcatcgatct gtccaaaatt ttctttgatt
    90721 ccgatagact gggtatgaaa atctgagaca ctcaatccta tacccaataa gataaaaagg
    90781 aaaatcccca agatcttgat gattgagaaa aaagattcca attcaccaaa ccacttgact
    90841 gcaacaaggt tgactgccat taagatcatt agtgcgctac ctccgcttat caaagctggt
    90901 acatgtacaa accagaaatg gatatacaat cctattgctg ttaattccgc aagtccagct
    90961 ccaagccagc aaagccaata agtccaattg acaaaaaatg cccacttttt gcctaaataa
    91021 tagtcaacga aatcactgat agagttaaat gtactatttg ctaaaaataa atcacccatg
    91081 gcccgcatca aaccataaat agccaaaccg catatcaaat aaacgagaat aacagctggc
    91141 cctgctgatg caattgcctt tgctgaacct aaaaataatc ctgttccaat tgcgccacct
    91201 aatgcgatta actggatatt tcgattagtt agagtccgtt tcattgccgg tttttccatc
    91261 aagttcacct ttttcttttt caagcagcat ggcgacttcc tgttcccaag aaggtttctt
    91321 tattttttca atacagagtg gtactacaaa caaaatgaca ctgatggctg tgactgcaat
    91381 tagattgatc catgttccag ctttcatcgt cagtccgaca gaaaatccga ttgccgcaaa
    91441 taaaacaata acgacagtcc aaactccaag attgctcttt ttctcttttt ttccaattct
    91501 aaatggtcga ttcaactttg catgtgtata acgcaatctc aaaactgcga tccccatgat
    91561 gacatagacg atacagtaga taacagtagt tgaatttacg agcatcaaaa atgcttgatt
    91621 gacgccagga atcagcaaat aaaaaagtgc aaagattgag ataatgacag actgagtcaa
    91681 aataacggat ttggatacgt caaattgatt cgtcttccaa aaattgaatt ttggtggata
    91741 cgcacctttt ctagcacttt ctgtcactgt ctttgctggt cctgaggccc aagcagataa
    91801 ttggactgct acaccaatga agacaagtaa actaaacaga ttcacaataa gatgtggcaa
    91861 accaagaatt tggcaataaa tcgaaataga ttgcgcgata ttattcaatt ccatttgtcc
    91921 ttttggtacg acatttgcta caactaaagc attcaaaata ttgacgaaaa atataaaaat
    91981 taacgcggca aatactccgc gaatatacgt tttaactgga ttttccaatc gagtgatata
    92041 cacagaggac atttcgatac cagtaaaaat aaacatgatc ggcgcaaaat aaacaaatga
    92101 ggatgcagtc gtagtatctg gtattaattt gtcccatgaa aaactgccta atgttccggt
    92161 tggtacgatc ccaactttga tccacgcagc aaaccctaat aagagcatca tcgtcaaagg
    92221 cacgtacaat cctaaccaaa caccgacttt tccgccgatt tttgccatat caaatttcaa
    92281 attcaaaaga gtgatgatcc aataaacgac taaaatgaca actaatgtaa atttgctgtt
    92341 caaaccaagc ggcacattat cgataatatt accaaacaac ggagcgagtg ctgaagcgac
    92401 catcaccatc ccaggaaaca tttggaccca taacagccat gagacaacga acccccattt
    92461 ttttcctaga gcattcgata cccaaagttc tggaccacct ttttggggaa acattccagc
    92521 aaattcacca gagatcaatg agattggaaa agcgtacaat aatactgcaa ccagcatgta
    92581 aaaaatcatc gtccagctcg ttgctgctac atctggaata tttcgtacac ttgctacaag
    92641 ggcgcaggtc attccgataa aggtcatggt cgtgatttgc tttgatgaac cagtatcttt
    92701 catctttatt ccttctttca atagcgacat atgtttttgt tttcttttat ttttgttcaa
    92761 gaacagaaat gacaaggttt catcgattca gtacaaaacc cagtatcacc gaaacatcca
    92821 ttgaacgatc ttagtgaact gggtttgata catgaaacga acgatccaac ttatagaacg
    92881 ttttataaaa aagaatatct tggcgaggaa tatatgaaaa acagggggaa atacaattgt
    92941 tttgatgaag caaataattg tctgttctcc atatgtctct tgttattctt tcacacaaaa
    93001 atcagttcta ttggatgatt tcgtttagtt tcttttccat tgctttggcg attttttttg
    93061 tataaaaatt gaaaattcga tcatcattca aataaggagt catgatagcc gcacgtagca
    93121 gagtgatttt ctgttctttt ttccattctt ctttcgttag acccattctt tcaacaaaac
    93181 gaatcggaga atcaccgtaa tcttcttgtg taaatgtagt atgagaagta ataaaccgtt
    93241 caccataaac atcgccatcc atataagaag accgatcgaa cattttttca tttaattcat
    93301 tgatagcgtt caaatctgta cagccttgtt ctttgaatac ccaattgacc atattaaaat
    93361 caggatgatt caatggatag acttcgattg tttttccttt aacagtaaaa gtcagatgat
    93421 cgagaaattc tctgaatctt tgagctgcct cgattgaagc cccaatcaat tgaccatatc
    93481 cggtcacatt caatggcaat acacgatgtg ctgtccagac tgcggcggcg gttgctccag
    93541 cttttgatcc ttccaaaata taagctccta acatatctgg agcctttact gatttttcaa
    93601 acacatatgg cgcgaaataa gaaatgatat ttctcatatt tttatgtttg attacgattc
    93661 ctccagcagc ataagggaca tagcccatct tatgaggatc tatcgtgaca gaatccgctt
    93721 ctgaaatagc tctgaaccct tcatatacct ctttgtcaat cgttacgcag tggtggaaaa
    93781 catgatgttc ttcaaagaat tcagctaagg aagcatacgg cacaaattct cctgcttcgt
    93841 ttagaaacag tgaacgcgca tagccgccat atgcggcatc aacatgtaaa tagaaataga
    93901 tcccttcatc ttttaacctt tctcttaatt ggacgatttt atctacactg tctacttgac
    93961 cttcttctgt cgttcctacg acagcaacca cacctagaat tgggattttt tgtccagcta
    94021 attcacgaat tgttttttcc aaagcattaa tatccattcg ataatcctct tgaactggga
    94081 tagcaaccat ctgatctaag ccgactccgc aaatatctaa cgccttcatc caagaatagt
    94141 gttttgtttg agggaccagc catttcccta atctttggat attttttccg ctacgggaag
    94201 aagctgcttt gacctcatcc atttcttcgt ccgtaaagct ttcggtcatt tccaaaattt
    94261 cttcaacaga taaattcaaa agttcccact cagacatttt tttcactttt tcaggcagca
    94321 cttccttgac agctaaagga atcgatttga tgcaacgggc gtaccataat ccttcaagat
    94381 tagcaataga accatctgct gcaatgtgtc cccatccgtc tgtcatattg aataatgaag
    94441 caaaatcctg tcctacttct gcttccattt gcgaagttgc catagaagat tccaatgcaa
    94501 cattatttgg gttccacagc atcgcatagt tgtaagctaa taaagcaggc atcaatgttt
    94561 cagcattcat ttgtccccag taacggcctg ctgaatgcca tgggatcgat cctgcgcgta
    94621 atcgctgact gacttcatcc aagacctcta tcatatggtc gcgggtggct agataatctg
    94681 gagtgagctt atctccttcg ctaattgcag gcatatctga aggaatataa ttttctctcc
    94741 atccaagatg ttcatctacc atcttgttca acaacatttt ataaactggt ccattttcag
    94801 ctttgtcgcc tataaagacg gccttgatat ccatatcttt catttttctt ccctccatat
    94861 tttgttggtc aatgtcttgc agatacatgt gtttatctgt aacacctctt cactatagcg
    94921 ccaaaaatga gaaatttttt tttataacat caaatccacg tagattttta caaacaaaaa
    94981 taaataaagt cggatttttc tgataattca acgcaaaaaa gcgaacaatt tttcaataaa
    95041 tcagtcttct aaaatagata acctaaaaaa agcgaacaaa caaataaaat gactgtcctt
    95101 cattcactaa acatattttt atttttttat attgttaaaa at
//
LOCUS       DARBFD010000002        84918 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000002 DARBFD010000000
VERSION     DARBFD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.002"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1589)
                     /locus_tag="SCK08_000080"
     CDS             complement(<1..1589)
                     /locus_tag="SCK08_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1200002.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
     gene            complement(1604..5125)
                     /gene="mfd"
                     /locus_tag="SCK08_000081"
     CDS             complement(1604..5125)
                     /gene="mfd"
                     /locus_tag="SCK08_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEG1200003.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(5142..5702)
                     /locus_tag="SCK08_000082"
     CDS             complement(5142..5702)
                     /locus_tag="SCK08_000082"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEG1200004.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            6055..7029
                     /locus_tag="SCK08_000083"
     CDS             6055..7029
                     /locus_tag="SCK08_000083"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1200005.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            7199..8599
                     /locus_tag="SCK08_000084"
     CDS             7199..8599
                     /locus_tag="SCK08_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEG1200006.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(8677..9399)
                     /locus_tag="SCK08_000085"
     CDS             complement(8677..9399)
                     /locus_tag="SCK08_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1200007.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(9478..9984)
                     /locus_tag="SCK08_000086"
     CDS             complement(9478..9984)
                     /locus_tag="SCK08_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1200008.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            10107..11906
                     /locus_tag="SCK08_000087"
     CDS             10107..11906
                     /locus_tag="SCK08_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEG1200009.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(11974..13548)
                     /locus_tag="SCK08_000088"
     CDS             complement(11974..13548)
                     /locus_tag="SCK08_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1200010.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(13803..14312)
                     /gene="mreD"
                     /locus_tag="SCK08_000089"
     CDS             complement(13803..14312)
                     /gene="mreD"
                     /locus_tag="SCK08_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEG1200011.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(14316..15170)
                     /gene="mreC"
                     /locus_tag="SCK08_000090"
     CDS             complement(14316..15170)
                     /gene="mreC"
                     /locus_tag="SCK08_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEG1200012.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(15325..15963)
                     /locus_tag="SCK08_000091"
     CDS             complement(15325..15963)
                     /locus_tag="SCK08_000091"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEG1200013.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(15964..16614)
                     /locus_tag="SCK08_000092"
     CDS             complement(15964..16614)
                     /locus_tag="SCK08_000092"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEG1200014.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(16627..17526)
                     /gene="rsgA"
                     /locus_tag="SCK08_000093"
     CDS             complement(16627..17526)
                     /gene="rsgA"
                     /locus_tag="SCK08_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEG1200015.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(17691..19760)
                     /gene="pknB"
                     /locus_tag="SCK08_000094"
     CDS             complement(17691..19760)
                     /gene="pknB"
                     /locus_tag="SCK08_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEG1200016.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(19757..20497)
                     /locus_tag="SCK08_000095"
     CDS             complement(19757..20497)
                     /locus_tag="SCK08_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEG1200017.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(20510..21868)
                     /gene="rsmB"
                     /locus_tag="SCK08_000096"
     CDS             complement(20510..21868)
                     /gene="rsmB"
                     /locus_tag="SCK08_000096"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEG1200018.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(21868..22806)
                     /locus_tag="SCK08_000097"
     CDS             complement(21868..22806)
                     /locus_tag="SCK08_000097"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEG1200019.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(22799..23290)
                     /gene="def"
                     /locus_tag="SCK08_000098"
     CDS             complement(22799..23290)
                     /gene="def"
                     /locus_tag="SCK08_000098"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1200020.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(23305..25713)
                     /gene="priA"
                     /locus_tag="SCK08_000099"
     CDS             complement(23305..25713)
                     /gene="priA"
                     /locus_tag="SCK08_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEG1200021.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(25966..26271)
                     /locus_tag="SCK08_000100"
     CDS             complement(25966..26271)
                     /locus_tag="SCK08_000100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1200022.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(26276..26890)
                     /gene="gmk"
                     /locus_tag="SCK08_000101"
     CDS             complement(26276..26890)
                     /gene="gmk"
                     /locus_tag="SCK08_000101"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEG1200023.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(27377..27661)
                     /locus_tag="SCK08_000102"
     CDS             complement(27377..27661)
                     /locus_tag="SCK08_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200024.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(27679..28569)
                     /locus_tag="SCK08_000103"
     CDS             complement(27679..28569)
                     /locus_tag="SCK08_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEG1200025.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(28589..28807)
                     /locus_tag="SCK08_000104"
     CDS             complement(28589..28807)
                     /locus_tag="SCK08_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEG1200026.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(28777..30477)
                     /locus_tag="SCK08_000105"
     CDS             complement(28777..30477)
                     /locus_tag="SCK08_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEG1200027.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            30744..31517
                     /locus_tag="SCK08_000106"
     CDS             30744..31517
                     /locus_tag="SCK08_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200028.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(31598..32167)
                     /gene="thiT"
                     /locus_tag="SCK08_000107"
     CDS             complement(31598..32167)
                     /gene="thiT"
                     /locus_tag="SCK08_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEG1200029.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(32233..32335)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(32341..33312)
                     /locus_tag="SCK08_000108"
     CDS             complement(32341..33312)
                     /locus_tag="SCK08_000108"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1200030.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(33406..34779)
                     /gene="glmU"
                     /locus_tag="SCK08_000109"
     CDS             complement(33406..34779)
                     /gene="glmU"
                     /locus_tag="SCK08_000109"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEG1200031.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(35282..36100)
                     /gene="purR"
                     /locus_tag="SCK08_000110"
     CDS             complement(35282..36100)
                     /gene="purR"
                     /locus_tag="SCK08_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEG1200032.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(36267..37091)
                     /locus_tag="SCK08_000111"
     CDS             complement(36267..37091)
                     /locus_tag="SCK08_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1200033.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(37064..37744)
                     /locus_tag="SCK08_000112"
     CDS             complement(37064..37744)
                     /locus_tag="SCK08_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1200034.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(37767..38705)
                     /locus_tag="SCK08_000113"
     CDS             complement(37767..38705)
                     /locus_tag="SCK08_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEG1200035.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(38950..39813)
                     /locus_tag="SCK08_000114"
     CDS             complement(38950..39813)
                     /locus_tag="SCK08_000114"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEG1200036.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(39955..42528)
                     /gene="mprF"
                     /locus_tag="SCK08_000115"
     CDS             complement(39955..42528)
                     /gene="mprF"
                     /locus_tag="SCK08_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1200037.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            42669..43475
                     /gene="proC"
                     /locus_tag="SCK08_000116"
     CDS             42669..43475
                     /gene="proC"
                     /locus_tag="SCK08_000116"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEG1200038.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(43554..45110)
                     /gene="rny"
                     /locus_tag="SCK08_000117"
     CDS             complement(43554..45110)
                     /gene="rny"
                     /locus_tag="SCK08_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEG1200039.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(45521..46570)
                     /gene="recA"
                     /locus_tag="SCK08_000118"
     CDS             complement(45521..46570)
                     /gene="recA"
                     /locus_tag="SCK08_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEG1200040.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(46679..47920)
                     /locus_tag="SCK08_000119"
     CDS             complement(46679..47920)
                     /locus_tag="SCK08_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEG1200041.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(48160..48738)
                     /gene="pgsA"
                     /locus_tag="SCK08_000120"
     CDS             complement(48160..48738)
                     /gene="pgsA"
                     /locus_tag="SCK08_000120"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEG1200042.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(48947..49870)
                     /locus_tag="SCK08_000121"
     CDS             complement(48947..49870)
                     /locus_tag="SCK08_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1200043.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(50609..51895)
                     /locus_tag="SCK08_000122"
     CDS             complement(50609..51895)
                     /locus_tag="SCK08_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1200044.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(51888..53156)
                     /locus_tag="SCK08_000123"
     CDS             complement(51888..53156)
                     /locus_tag="SCK08_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1200045.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            53408..53869
                     /gene="mscL"
                     /locus_tag="SCK08_000124"
     CDS             53408..53869
                     /gene="mscL"
                     /locus_tag="SCK08_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEG1200046.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(53929..54375)
                     /locus_tag="SCK08_000125"
     CDS             complement(53929..54375)
                     /locus_tag="SCK08_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1200047.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(54468..54713)
                     /locus_tag="SCK08_000126"
     CDS             complement(54468..54713)
                     /locus_tag="SCK08_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEG1200048.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(54828..55406)
                     /locus_tag="SCK08_000127"
     CDS             complement(54828..55406)
                     /locus_tag="SCK08_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1200049.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(55483..56013)
                     /locus_tag="SCK08_000128"
     CDS             complement(55483..56013)
                     /locus_tag="SCK08_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEG1200050.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(56017..56877)
                     /gene="rlmB"
                     /locus_tag="SCK08_000129"
     CDS             complement(56017..56877)
                     /gene="rlmB"
                     /locus_tag="SCK08_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEG1200051.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(56870..57265)
                     /locus_tag="SCK08_000130"
     CDS             complement(56870..57265)
                     /locus_tag="SCK08_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEG1200052.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(57262..58674)
                     /locus_tag="SCK08_000131"
     CDS             complement(57262..58674)
                     /locus_tag="SCK08_000131"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEG1200053.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(58671..59207)
                     /gene="cysE"
                     /locus_tag="SCK08_000132"
     CDS             complement(58671..59207)
                     /gene="cysE"
                     /locus_tag="SCK08_000132"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEG1200054.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(59293..59532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(59529..60989)
                     /locus_tag="SCK08_000133"
     CDS             complement(59529..60989)
                     /locus_tag="SCK08_000133"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEG1200055.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(61159..62316)
                     /locus_tag="SCK08_000134"
     CDS             complement(61159..62316)
                     /locus_tag="SCK08_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEG1200056.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(62350..63720)
                     /gene="radA"
                     /locus_tag="SCK08_000135"
     CDS             complement(62350..63720)
                     /gene="radA"
                     /locus_tag="SCK08_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEG1200057.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(63783..64310)
                     /locus_tag="SCK08_000136"
     CDS             complement(63783..64310)
                     /locus_tag="SCK08_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEG1200058.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            64540..65346
                     /gene="proB"
                     /locus_tag="SCK08_000137"
     CDS             64540..65346
                     /gene="proB"
                     /locus_tag="SCK08_000137"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEG1200059.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            65343..66590
                     /locus_tag="SCK08_000138"
     CDS             65343..66590
                     /locus_tag="SCK08_000138"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEG1200060.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            66666..67010
                     /locus_tag="SCK08_000139"
     CDS             66666..67010
                     /locus_tag="SCK08_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200061.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(67127..67453)
                     /locus_tag="SCK08_000140"
     CDS             complement(67127..67453)
                     /locus_tag="SCK08_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200062.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(68068..69270)
                     /locus_tag="SCK08_000141"
     CDS             complement(68068..69270)
                     /locus_tag="SCK08_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1200063.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            69387..69902
                     /locus_tag="SCK08_000142"
     CDS             69387..69902
                     /locus_tag="SCK08_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1200064.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(70101..70547)
                     /locus_tag="SCK08_000143"
     CDS             complement(70101..70547)
                     /locus_tag="SCK08_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG1200065.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(70594..72072)
                     /gene="msr(C)"
                     /locus_tag="SCK08_000144"
     CDS             complement(70594..72072)
                     /gene="msr(C)"
                     /locus_tag="SCK08_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1200066.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(72426..73055)
                     /locus_tag="SCK08_000145"
     CDS             complement(72426..73055)
                     /locus_tag="SCK08_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1200067.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(73039..73503)
                     /locus_tag="SCK08_000146"
     CDS             complement(73039..73503)
                     /locus_tag="SCK08_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1200068.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(73990..75258)
                     /locus_tag="SCK08_000147"
     CDS             complement(73990..75258)
                     /locus_tag="SCK08_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1200069.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(75489..76226)
                     /locus_tag="SCK08_000148"
     CDS             complement(75489..76226)
                     /locus_tag="SCK08_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1200070.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(76293..76862)
                     /locus_tag="SCK08_000149"
     CDS             complement(76293..76862)
                     /locus_tag="SCK08_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG1200071.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            76990..77862
                     /locus_tag="SCK08_000150"
     CDS             76990..77862
                     /locus_tag="SCK08_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1200072.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            78204..79076
                     /locus_tag="SCK08_000151"
     CDS             78204..79076
                     /locus_tag="SCK08_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1200073.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            79073..81031
                     /locus_tag="SCK08_000152"
     CDS             79073..81031
                     /locus_tag="SCK08_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1200074.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(81363..82214)
                     /locus_tag="SCK08_000153"
     CDS             complement(81363..82214)
                     /locus_tag="SCK08_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1200075.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            82337..82804
                     /locus_tag="SCK08_000154"
     CDS             82337..82804
                     /locus_tag="SCK08_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEG1200076.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(83067..83549)
                     /locus_tag="SCK08_000155"
     CDS             complement(83067..83549)
                     /locus_tag="SCK08_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEG1200077.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(83649..84512)
                     /locus_tag="SCK08_000156"
     CDS             complement(83649..84512)
                     /locus_tag="SCK08_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1200078.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        23985 a        18210 c        15313 g        27410 t
ORIGIN      
        1 ttaatttttt tatttaatcg caggattttc ttaccaaaag gcaacattag ccattctcgt
       61 actgtaaaca gacgaagacg aatcactgca taaatgaaca cagaagcccc gatcccaact
      121 cctatcacgc tgacaaaaaa agcagtggaa cgatgagtta caggaccaaa tactatattc
      181 aatacaccat aatacagcag cagtactact gtcatacaaa gaaggctgaa catcaacttc
      241 cgtccaaaat gccgttcttt ccaaaaagca tttagctctt ttgtttcaat tcgaacaaaa
      301 taccaaagcg tcacacctaa tcctaaaagg gtagacaggc ttgcacctgc tgttcctaat
      361 aaaccggtca gaaaagaagt agtcaatcct tttaccaata gtccccatcc tgcgcctttt
      421 aaagaagggc gaaacgagtt cttgctttga gcaatacttt gataagcttg gatgactgca
      481 gtgaaagcaa tggaaaaaac aaataataca agcgctgcat ttcccgcata atctttaaac
      541 aacgcataat tgatataagg gagcagcaag gctaatccca aagacgcagc taatgctaaa
      601 gcggtcgtca atctaagata gattttggct gtctgtaaaa actgacgata gatccggttg
      661 gtcaaatgac gagtcaacgc aggcaggaat gtcgcactta atgcagtggc aatcactaaa
      721 cctaattgta ctaaaggctg acctcgatca tagacccctt ttgctatctt cgcaccgtat
      781 tctgacagtc cgtaaacttc caaagcattt tttacaaaga aagagtcgat cagctggaaa
      841 aagataagca atccactata aatcgaaacc agtccgcctt ctaccagaaa acgccggatc
      901 aagggctttg acggctgttt gaacaatgga aaccgtcgga agcttaacgc tcctccatga
      961 atcttttgtt catagtagtg aaggatccca tatgcgcata aaccgccaaa aacagctcca
     1021 ctcattgctg ccgtccctgt ttggtacacc gtccatccaa gctttctaaa agcaaacgca
     1081 ctacagacaa tcacaaaaac acgaacgaac tgctcaacta cttgagaaac agcagttggt
     1141 tccatcaaga atcttccttg gaaattccct cgataaaaag atagaccagg catcagcaaa
     1201 aaggtaaagg aaactacttg gatcagcggt ttcaattgta tgtctcccat catccaagca
     1261 atccagctgc taaatgcaaa gacaaacaac cacaaaaaga cacctgtcca aaatactaag
     1321 ggataaagat tttgcaaagc ttgctttcgc tcgaaaggat tctttctttc tgcaacgtac
     1381 tttgatatga actgcggaag accggataaa gctaaagtca ttgctatccc ataaattgga
     1441 taaacttgct gataaacata aaaaccttcg tcgccgacta agttttggag gggaattcgg
     1501 taaagggcac ttaatacttt tgctatgaaa gaagcaatcg tcaatacaaa tgctccttgc
     1561 atgacgcgcc gcatttcttt atgatccaaa tcattgctcc ttcctacgct gacagatatt
     1621 tttgctcacg caacgctttg acaaactttt ggatttcctg aagccatact gcttcttcca
     1681 tttggttggg gatcgtcaag cgtatcacca tctgttcttt ttcaacgcct agactggctt
     1741 ttagttttgt cgctgtcagt gcttcgaata actgttccac actatagcgt tttgttccaa
     1801 ctttacttag agtaaagact atatgcgcct cctgtttcct tatcgtttct aggagcgctc
     1861 gatcgccatc cattttgatc tgcccaattg tcaagagatg tgctacttct tctggatatt
     1921 ctccaaaacg gtcgagcaga tcatcttcta gttcatctag catttcttgc gaatcaagtt
     1981 cacgaattcg tttatagatc tcgattttct gccgttgatc tgccacgtat gtttcaggta
     2041 gatacgcatc gacaccgagg tcgatctcaa cagtggtctt ctccacttga ctattctttc
     2101 cttgtttacg agaaacagct tcgcttagca tttgagaata catatcgaaa ccgactgcat
     2161 caataaatcc atgctgctga gcacctaaga gattacctgc accgcgaatg gaaagatccc
     2221 gcatcgcaat cttgaatcca gatcccagtt ctgtaaaatc tttgattgcc tgaagacgtt
     2281 tttcactgac ttcgttaagg atcttctgct gctcatacat aaagtaagca tatgcgacac
     2341 ggctgcttct tcctacccgc cctcgcaatt ggtataacgt ggataatccc atataatcag
     2401 cgttctcaac gaacaacgta tttgcgttcg ggatatctac tcctgtttca atgatggttg
     2461 tagtgacaag aacatcatat tgaccttcaa taaattcaaa aagcgtattt tctaattgcg
     2521 cctctgtcat ttgcccatga gcataaccga tccgtgcttc cggaacgagt tcttggattt
     2581 cttctacttt ttgttcgatc gtttctaccc gattatataa atagaatact tgtccacctc
     2641 gtcccatttc tcgatggatt gcttcgcgaa tagccccagg atttttttcc ataacatacg
     2701 tttggatcgg ataacgattc gcaggcggtg tttcgattac tgaaagatcc cgcacgccta
     2761 acatcgacat atgcaaagtt ctcggaatcg gtgttgcagt cagagtcaaa acatccactt
     2821 gtgcacgtaa ctgtttcaat cgttccttat gttttacacc aaaacgttgt tcttcgtcaa
     2881 tgatcagtag acccaaatcg ctaaattcaa tatcttttga caaaattcgg tgggttccta
     2941 caacaatatc aacttgacct gtacgcagtt gttcgatggt ttctttttgt tgctttcttg
     3001 ttctgaaacg acttaatagc ccgatattta cggggaaacc ttcaaatctt tccagcatcg
     3061 tttcatagtg ctgctgtgcc aagatcgttg ttggaactaa aaaggctact tgtttgcttt
     3121 cctttatcgc tttaaaggcc gctcgaagcg ctacttctgt tttcccataa ccaacatcac
     3181 ctacgagtag tcgatccatt ggtttttctt tttccatatc ccgtttgatc tccgctgcac
     3241 ttctcaactg gtcatcagtt tcagagtagg ggaaggcatt ttcgaattct ttttggtagg
     3301 cgtcatcagg tccaaaagca tatccttttt ctgactctct tgcggcatat aatttgatta
     3361 gatcgtcagc gatatcctcg atttttgaag agacttttcg cttggttttc gtccattcac
     3421 ttccgcctag cttattgatg cgaggcgctt tggcttcaga ggcgacatat ttttggatca
     3481 gattcagttg agtaacagga ataaacagtt tgtcgtcgtt ttgatacaaa atagtcatat
     3541 agtcttggtg aacgccatct acttctagcg tttccatccc aatgtatttc ccgataccat
     3601 gattggcatg gacaacgtaa tctcctgctt ttaactcatt atagcttttc aatcgctctg
     3661 cgtttgttac tgtttgacgt ctcgtttgtt ttttcgtcgt tttttggaaa atttcttttt
     3721 ctgtgatagc tactattttt tcttgaggaa gttcaaaacc agcctgcaaa gctccttcaa
     3781 ctaattgtac ctgtcctctg atcaatgcat ttttttctgt ttctactact aatatgtctt
     3841 catctcgaag catttgttct gctttgcgta ttctgtcctt tgtcggaata aagatcacaa
     3901 cagtctgttt ttgcttgcgc caacggtcca tttctgtttt cagcaatggc atttgtccga
     3961 agaactgttg catcgtccga tattgaaaat tatggattgc ttggaaacga agatttccca
     4021 tccctttttg gaagagtgag aaaaaggttg tggtgaactt ttccttctgc acgatttttt
     4081 gtatatctgc tgaaaatgtc tgctcagaaa acacccgcaa ttcgctgatt ttttgagtct
     4141 gccattctgc cgcttccctt tcgatttccc gatttgtttc catgattcgc tgataatcat
     4201 caacgactag taaactattt gaagggaaat aatccaagat cgtcacagat tcttggtaaa
     4261 gaagatctga gtagaatttt gcttcttctg tcgggacacc tgcttgccag cttgaagtca
     4321 gctgaccgaa gtaatcttgc aaaaactcgc ggtctgcttc ttctgtggtc gcagcaagcc
     4381 gtttttctag cattttttgc atttgctcta ttccatgttc tagatcctct ggtccgtaga
     4441 cttgttcact cgttggggaa acccagattt cttccgtttt ttcgattgag cgttgtgttt
     4501 ctgcttcaaa ataccgcata gaatctatct ctacatcaaa tagttctaca cgaacaggat
     4561 aggttgtcgt taacgggtaa atatcgataa tgcttcctcg aatactgaat tctcccggtt
     4621 ttgcgaccat ctcttctcgt acatagccca tcaaagtcaa ttggcgagga agattttcca
     4681 cgtctatctc atcaccaata tgccaattca attgcgcctg tagccaagtt ttcttattcg
     4741 gcagtcgttt gtgcaaagat gcgactggca acaaatagat acctgctttt ttttcttgaa
     4801 gtgcgttaag cgttgcaact ctctcgcctc gcgcttctgg agaagaaaag gccatttcag
     4861 cagaaagcac ttcatccact ggaaataaat ggatctgatc ttgcggaatc acatgttgca
     4921 gatcttctgc taactgattc gcataataca aatttggcat cagaataatc atgtttttat
     4981 ttgttttctt aaacgctcga gcaaatagaa gtgttttcgc tgagcccgct aatcctgtga
     5041 ttagttgtct aggcattttt tcttctaaat tgctgaccca ttcttttact tgcggatctt
     5101 ttccgactaa ttgaataata tccattcctt ttcctccttc ctcaattgaa tcgattcatc
     5161 gcatccacga acgaatgccc ttctatgaga tattcgactg ctttaacaga ttgacacatg
     5221 ctttcttcta tcaaaggctg attttctttg ctgaaaggac taagcacatg ctggactact
     5281 gtttttttcc cttctggcct gccgattccc accttgattc gatcaaagac attcgtattc
     5341 aaatgggaaa taatactttt gataccgtta tgcccgccgg cacttccctt ttgacggagc
     5401 cggatttttc ccactgccag atccaaatca tcgtagatta cgactaattc ctctggataa
     5461 atcccaaaat aagtcattag tgggccgact gcacgaccag attcattcat gaatgtttgt
     5521 ggtttgacta aaaggatctt ttctccatta tgaaaaaatt cggcaacttc tgcttcgaac
     5581 ggattttttt tgaaagtagc ttgatgctcg cgtgcaattt tatccacgac cataaagccg
     5641 atattgtgtt ttgtatattg gtatttggta cctgggtttc ctaacccgac gatcattttc
     5701 atttttgttt tccacctaat tctaaaaagc agcacaaaag gctggactgc taattagcgg
     5761 tccagtccat cgagcttttt tcttttttac acttgtcaat gcatagtata acacaaaaca
     5821 aggcgtttcg gctaaaagag aatcgttaat aaatgataaa gaaattaaaa atttcttcaa
     5881 agtttaatga aataattgat atataaaaat agtgaaataa cgcacatgta agcttttaca
     5941 tataatacaa ccttgttatc tgttatattt tatagccaaa tagcgtgaca tggggcttga
     6001 gaaatgttag cataagcatg ggaaaaaaca aatcaatgga aggattgata ctaaatgact
     6061 gcagctgcag aaaaaaaaga tcatcaaaaa gttattcttg ttggagacgg cgccgtaggt
     6121 tctagctatg cctttgctct agttactcaa aatatcgctc aagaagtagg tattgtggat
     6181 atcaatactg ctaaaactga aggagacgca attgacttgt ctcacgcttt ggcatttact
     6241 tcacctaaaa aaatctattc tgctacttac gcagacgcac atgatgctga cttagttgtt
     6301 attacagctg gcgcaccaca aaaaccaggt gaaactcgtt tagacttagt aaacaaaaac
     6361 ctaaaaatca accgcgatgt tgtcacacaa atcgttgaat caggatttaa cggtatcttt
     6421 ttagttgccg ctaacccagt agatattttg acttactcaa cttggaaatt ctcaggtttc
     6481 cctaaagaac gcgtaatcgg ttcaggtacc tctcttgact cagctcgttt ccgtcaagca
     6541 cttgcggaat tagttgacgt tgatgcacgt aacgtccatg cttacatctt aggtgaacac
     6601 ggagattcag aattcccagt ttggtcacat gcaaatgttg ccggcttgca aatctatgaa
     6661 tgggtaaaaa acaacccaga aatcgatgaa gaagctatgg ttaacctatt ctttggcgta
     6721 cgtgacgctg cttacacaat tatcgagaaa aaaggcgcta ctttctatgg tatcgcagta
     6781 gcattagcac gtatcactcg tgccatctta gatgatgaaa atgctgttct tcctttatca
     6841 gtattcatga atggcgaata tggattgaac gacatctaca ttggtgcacc tgctgttatc
     6901 aaccgtcaag gtatccaaaa agtaatcgaa attcctttaa gtgacagcga gcaagaccgt
     6961 atggctgctt ctgctaagca attaaaagaa attctagatg aagcttttgc taaactagac
     7021 gctgaataat ctttgaacat gacggaaaac atctattcgg tgttttccgt ttttttattt
     7081 tatcaccctt atggtttcta aattttttca taaagtagag ataaccttcg tcttttcctc
     7141 taaattatgc tatcatgaga ccgttccacc ctaaactttt acttaaggag ttgtcgatat
     7201 gacaagatct tcgcatcgaa aaaaatcgaa acatgttgct ttgctttcag ttcttggtgt
     7261 tctcttagtg ctgatcagct tttataccta tcgcagtaca tactacacaa agcatttttt
     7321 gccgaataca gaaataaatg gaataaatgt cagcaatcta acagtcgaaa aagcaaatga
     7381 aaaattaaaa gaagcttatt ctgataaaaa actggctatt aaagaagatg gaaaagtctg
     7441 gcaggaagta ccaaaatccg agctcggcta caaagatgat ttcacttcgg agctttctcc
     7501 gatcctaaat gaacaaaacg gatggacttg gggaatgact tatgtctcag cggcagaaaa
     7561 acaagaaata gatcctgtca gtcaaaacca ccaaaaacta gacactaccg ttcagacatt
     7621 gacaactaag ctgactgatt tgaacaaaga ccggacacct actactgatg caacgatcga
     7681 aaaagcaggt gactctttcg ttatcaagcc tgaagtcaat ggaaatacga tcgacgtcga
     7741 tgctgcggtc aaacaattaa aaagtgcggt aaactctggt aaagatacaa ttgaattgac
     7801 tgaattcaaa gaaaaaccaa aagtaacatc tgaagacagc tcactgaaag aacaactcgc
     7861 atccatgaat gctatcgcaa atgtaaaagc tacttacagt ataaacggtg agactttcca
     7921 gatcccatca agtgatatta tgagttggct gacctacaat gatggaaaag tcgatttaga
     7981 tacagaacaa gtacgtcaat atgtaacaga tttaggcaca aagtataaca caagtacgaa
     8041 tgacacaaaa tttaaaagta caaaaagagg agaagttacg gtcccagttg gcacttacag
     8101 ttggacgatc caaacagaca gtgagacaga agcattgaaa aaagcgattc tggcaggcca
     8161 agattttact cgttcaccca ttgtgcaagg tggcacaacc gctgatcatc cattgatcga
     8221 agatacgtat atcgaagttg atctggagaa ccagcatatg tggtattaca aagacggaaa
     8281 agtggccctt gaaacagata tcgtttccgg aaagccaact acacctacac ctgctggagt
     8341 cttctatgtt tggaataaag aagaagatgc aactttaaaa ggaacaaatg acgatggaac
     8401 accttacgag agtccggtaa actactggat gccgattgac tggacaggtg ttggtatcca
     8461 cgattcagac tggcaacctg aatacggtgg agacttatgg aaaacccgcg gttcacacgg
     8521 ctgcatcaac accccaccaa gtgtaatgaa ggaattattc gggatggtcg aaaaaggaac
     8581 gcctgtattg gtcttctaac aaaaataata tataaaaaga aagcacccgt acgattggta
     8641 caggtgcttt ctttttatta tctaacaatg tgctacttac atttcagcag cagtaaagac
     8701 ttctgaaaca tcatcatcgt cttctagctt atctactaga cgttccaatt gctctttttg
     8761 ttcctcattt aaatcaatcg tcgtttgtgg aatcatcgtc aattctgctt gcgccaaagt
     8821 gaactctttt tccaactcat ctcgtacagc agcaaaatct tctggtgcag tataaatctc
     8881 gaatacttca ggagaagttt gcagatcttc tgcccccgcc tctaatacat cctcaaacat
     8941 cgtctcttca tcaacagtca acccatcgcg ttcaatagcg atatatcctt tgcggtcaaa
     9001 caagtaactg actgaacccg tttctcccag tgatccaccg tttcgagtaa aagctactcg
     9061 gacatttgtc gctgtccgat tacggttatc tgtcaatgca tggactaaga ttcctactcc
     9121 acccggacca tatccttcat acgtgatttc atcgtaattc gtttcatcta cggcactcgt
     9181 agccttttta atcgcccgtt ccacattgtc gttaggcata tttgcagctt ttgctttgtc
     9241 cacaacaagc cgcaaagctg gattcataga tggatcagga ccgcctgctt tagctgccat
     9301 ataaatttca cgagaaagtt tctgaaagac tttccctcgt tttgcatctt gggcattttt
     9361 tcttccttgg atattatgcc atttactatg tcctgacatg atttttcctc ccttgttctt
     9421 ttgagaatac acttctattc tagtactatt tcacaaaaaa acaaagccac agccaatcta
     9481 taaacgcttc ttgaatgccg caaaaagcac acataatatc acagcagtca gagcaccgat
     9541 cgaatccaac atgacatctt ggaacatagg tgttcgtcca ccggtcatca tctgatggaa
     9601 ttcgtccaaa cctgcgtacc ctgttgctga aagccaagca aggatcgctg tcagccacca
     9661 aatttttagc ttggggaaaa cacctaaaaa caagctgcct cccaaaataa aataggtacc
     9721 gaaatgtgcg cccttcctta aaaagaactc taaaaatttg gcgtagccca gatggttgat
     9781 actcaccata cttccaccat aattaaaacg tatacccgct aatgtgtctt taaatggctg
     9841 attaggcagc cagctttcta atcgcgaaac ttgtgattgc tgtccgtaag tttgagaaga
     9901 actgtaaaat aagattataa cgactatcag tgcgattact aaataaatat ttccgttttt
     9961 catttgtttt tttgaagaat tcataagtca cctctgtatt ttatcttaga ctttttttga
    10021 agaaacaaca atcattttca tagagacttc ttccataaaa aagtgctata cttaattttg
    10081 tattacaata tgaagggagt tttttcatga gttattcatt aaattggaat ctagattcta
    10141 ttttttccgg aggcagtcat tctgatgctt tgaaccaacg gatgaagcaa ttggaagacc
    10201 aaatgaacga gtattatcac cgcgtaacaa aatggtcacc tagttctgac aatgcagagc
    10261 agttaaacgc tattcttcaa ttgcaagaaa cgattacgaa tggttttacc caatgcagca
    10321 gctatattac tgcattgctt tctgccaatg tcaatgattc agatgcaaag gtcttatcag
    10381 gcaagcttta cgcaatgctg cctcgtctgc aatcagccga aactgtattg tcaaagaaat
    10441 ttgcagaaat atctgacaat gattggaatc agatgctttc tcaatcttcg tttgaaacaa
    10501 ttgcttttcg tttaaacgag attcgtcgtg atggctcaca attattatca gaagctgaag
    10561 aaaacattat aaatacgctt tctttagatg gtctaaatgc ttggagcagt cattatgata
    10621 cgattgtagc cagcatttcg attcccttcg aacaagatgg acaagtagtc gaattatctg
    10681 ctggacaagc cttcaataag atgatgggcg atccagatcc aaaagtcaga gaaacacttt
    10741 tcgcagcatg ggaagaagcc tggaaagaaa aggcacccct cttcacggat actttgaatc
    10801 atttagatgg ttttagactt tctgactatg aacttcatgg tgtcacagat tttctacaaa
    10861 aaccattaga gtacaatcgg ttgaagaaag aaacattgtc tgtcatgtgg gatacaatcc
    10921 agaaaaacaa acaacccctt gttaattact tgactagaaa agctaatctt ttcggtaaag
    10981 aaaaaatgga atggcaagat caagatgcac ctattatttt aggcgatttg aaagaaaaaa
    11041 cctttagttt tgacgaagca gcagcattta ttatcgaaaa ctttaacaaa ttcagtccaa
    11101 aaatggctaa atttgcccaa tctgcttttg aaaagagctg gatcgaagca gaagatcgtc
    11161 ctgggaaacg tcctggtggt tattgtactg agttaccaga aacaaaagaa tcaaggatct
    11221 ttatgaccta cagtaattct gttaatgagg ttgcgacact tgctcatgaa ctaggacatg
    11281 ctttccatag cagcacgatg tgggatttac cttctttaaa tcgcgaatat gccatgaatg
    11341 tcgcagaaac agcaagtaca ttcgcagaac tgatcgtagc cgatgctaca ttaaaagtcg
    11401 caaaaacaaa agaagaaaaa atcaatttgc tagatacaaa actgcaaaat gctttagcta
    11461 tgttcatgaa tatccattcc cgattcattt ttgaaaatcg attctatgaa gcacggcaag
    11521 aaggattagt ctcagaagat aagatcacag agatgatggt tgaggcgcaa aaagaaggct
    11581 atcaaggagc tttagctact taccatccat atttctgggc tgcaaaattg catttcttta
    11641 ttgatgacgt accgttttat aacttcccat acacatttgg ctacctcttt agtttgggca
    11701 tctatgctta cgccaaccaa aagggagcaa attttgaaga agaatatatc catttactac
    11761 gcgatactgc ctcaatgacg acggaagatc ttgcgaagaa acatttgaat gtcgatctta
    11821 cccaaccaga tttttggcaa gcaggtatcg acatggtgac tgaagatatc caaacgttca
    11881 tggaattgac agaagaattt atctgatttc tattttatca caataaaaaa agagttagtc
    11941 cagaatgggc taactctttt tgattaatca ttcttacatg ctgacagcaa agtcaggggc
    12001 aaaccattga actgaaccaa cttttacgct ttcgcctggt tgaggagcat ggatatattg
    12061 tcctccgcct aaagcaatcg ctacgtggta agttccgcct tgtgaccccc agaataacaa
    12121 gtctccagct tttgcttgag aaactgaaat ttttgttcca gctgattctt gaggaactgt
    12181 ccaaccaccg atgtcacgac cagttacttg catgtaaaca tagcgtgtga accctgagca
    12241 gtcaaagcca cttggatctt taccgcccca aacatatgga gtaccaatat atttgtaagc
    12301 ttctgctacg attgctgcac cattcacact tccagaagga gctggagcag gtgttacagg
    12361 ttttggttgt tcaggtgttg gtgttggcgt tggagccgga gtactacttc ctgtgccgtt
    12421 tcctgtatca acactttgat ctgttgatgg tgttgtaggc gcaggtgttg ttgattcttc
    12481 tgttgttgaa gattcaggca ctgttgttga ttcttctgtt gctgaagatt caggcactgt
    12541 tgttgattct tctgttgctg aagattcagg cgctgttgtt gattcttctg ttgctgaaga
    12601 ttcaggcgct gttgattctt ctgttgaaga ttcaggtgtt gttgattctt ctgttgctga
    12661 agattgtgta gctgatgatg attcttcagt cattgaagat tgtgtagctg atgattcttc
    12721 tgttgctgag ctttgtgcag tagatgaagc ttgagtagct tgtgcttcag cctctgcttg
    12781 ttcgcgtgct tctttttcag ctttttcttg tgcagcttgt tgacgagctt gttctgctag
    12841 acgttgttgt tcacgaatac gagcttgctc tgcttcagct tctgcttttt gacggttcaa
    12901 gtcagctttc ttgtcttcag cagttgcttg ttcagcagct aatgaagttt tcagtacgtt
    12961 caaatcagct tgtttagata aaagatcgcc tttttgagat tccaaagcag cttggttttc
    13021 agctaattct tttagtttag catcgttttc agcttttttg tcttctacag cttttttatc
    13081 ttgtttttgt tgttccatca agtcgttgtt tgcttttacc atcgttgtca ttgcttgaac
    13141 gcgtccaata gcatctgcta atgaatcagc attcaataca gcatcgatgt agtttgagct
    13201 tgtgttgctt acttgtgctt cgcgtgcttg tttttggatt gtatcttcac gtttttcaat
    13261 acgttcttgt aaatctgcaa tgtcttttac taattgtgca gattcttgac gcaatgtatc
    13321 ttgttttgct aataggtctt gtgcttgtgt gttgatttca gaaacttggc tttctaaggc
    13381 atcgatttgt gattgtgcat ctgcttgttg atttttcaag tctgcaatct tttgatcttg
    13441 ctgttgtatc tgagaatcaa aatcgtccgc cgcggcagcg atcggtgatg ctacggcagt
    13501 caaggtcatt gaacatacca ttactgctga tattaaactc tttttcactc ttcattcctc
    13561 cgactggctt aatttaataa atatctaaat atatttaagt ctttcttaac gatataaaaa
    13621 ttgtaccata gcagtgtgtc aacgatataa tagttatgtt acaaaaatgt ttcaaaacaa
    13681 cttttaaaaa tgacaaaact gttcgtttca gaaaatgatc atgaaaaagc cgcgttaagt
    13741 ctagcatttc ttaagataac aacgattctt tttcgctttc gacctaaaaa taaaaaaaga
    13801 aattattcat cactgaataa tttcttaaag ggaaaaatga ataatacaaa tataatcatg
    13861 ttcaacagca gtgtaggtcc taaaaacctt gtaataaaat acgtgttatt tactactgct
    13921 aatttaaaaa tcaactggac caccatcgta aacaattcat aacccgtgac aaagatgatc
    13981 atactgaaaa attcagtaaa gatgttgaca tggataacgt ctttcattac atacatcaac
    14041 catacaatta aagggagagc tactgcatag ataccgataa cgccaatata gtaagcgtca
    14101 tagatagccc cgagaacaat cgtggtaatc agtaaataac gtttttcaaa cgccatactg
    14161 caacataata atgccaatat cagaaagtgg gcatttgaca tataggtgcc tttcgaccac
    14221 tcccctagca ttcttgttaa atgaccatct atcaacatca aaaagaacaa aacgattggc
    14281 agatagtact tcattgtttc tttcttcaac atggactaat cctccgctag ccgttggaca
    14341 atcgtcacaa ctgacacgtc atacatttct gcataaggtt tcacataaac ttcccgatcc
    14401 agcccataac tatctggttt cgttttaata accgtaccga tcggcaagtt agctggagaa
    14461 ttccctccaa gaccggatgt ctggacaaca tccccttctt tgatatccgt atcaccagtt
    14521 aattgggtca ccactaaggc atggagcttt tcatcatagt ttttaagcaa accaaacgct
    14581 tcgccattag ccgaagatac ccgtactgga aaatgattgg agctttcatt agaggatgac
    14641 agtaattcga ttttagacga agccgtattg acctcgatca ctcggccaat caatcctttt
    14701 tgcgacatga ctgccatatt tgcttcgata ccgtctttag aacccttgtc tacgatcagc
    14761 atgtcctgcc aactgtcagg cgaacgagta atgacatttg ccgttacttt ttcgtaacta
    14821 gtcagcgtct gattcaactc aagttctttt ttcaacgctt cgatttctct ctcgtaattc
    14881 ttgttttgct gtgaaacgtc atcatagcta tcgatttttt cttttagccg ctcattttcg
    14941 gcataagttg aaaaaagatt atggacagaa tctactccat ttcgtaccca ccgtactggg
    15001 gcagagattg ttttatcaac cattgcaaca ctgtcattaa cgatggattg gaagaaattg
    15061 ggctttcctt cattcgctcg ctgcgcagcg gtcacactta gtatcgtcac cacgatgata
    15121 acaataacca aggtaataat aatattttta ttaggattga acttcttcac aaagacacct
    15181 cgaattaatt agactcttgt gttcaatttt atcactaaat gccgtgaaac aaaaggtaag
    15241 ttgctcctct taagcttttt tttagcaaat aatagattct gtcattttta gcctgaaaga
    15301 caaatttttt tatttgttta tggatcaatc tttgctttga ataactgcaa tcataccatc
    15361 tgaaaaagaa aattctgcgg tatctgaaag aaattcgtta cttgcataag atgtcggata
    15421 ttctacttct ttattttcca atacatactt gctgcctttt aatgtaagat tcgagacggg
    15481 agtcaagcag caataagcta aatacttcat atctggtatc tgtcttacta tatgctctcc
    15541 aggcaaataa taagacaaat aattttgaca atctctgata ataagctgat gggcaaaggg
    15601 tttgaagcga ggttccatac ctaaccagag attggctaaa agatgatcca atcttccgcc
    15661 cgtagctcca atcaaagtga cttccgcttg cgggtaacgt tcaaatgttt ttaagatacc
    15721 taactgtgta tctgtatcgt ctttttctgc aggggaacgg gtatactctt tggcttttgc
    15781 aaatacttcc ttctgctctt cgttcgacaa tgaatcaaaa tcacccactg ccagatctac
    15841 tgggtagcct gcttgaagca ggtacagcgc accacggtct atcccgacat aggcatcgta
    15901 tttggacagc agagactgcg gccagttttg cggattacct ccagcagcta ataaaatttt
    15961 catttagtct aatgcctctt ttaatcgttc gatttgtccg actggatttt cggctccata
    16021 gatatatgat cctgcaacga agacatccgc ccctgcatct ttacagattt ttgctgtttc
    16081 tggaacgatt ccgccatcta cttcgattgc ataagtccaa ttgttctctc tacgtagatc
    16141 agcgagttcc gtaatttttt ctactgtttc ttcaataaag ctttgtccgc cgaacccagg
    16201 attgacagtc atgactagta cttgatcagc caaaggcaaa acatgtttga tcatggagac
    16261 tggtgttcct ggattgataa ctactccagc tttaactcct aagctttttg tcatttgaag
    16321 tgcccgatga atatgaggag ttgcttcgac gtggacagta atgatatctg ctccagcttt
    16381 tgcaaatgct ggaatgtagt tttctggatc aacgatcatc agatggacgt ctaacggtaa
    16441 tttagtaaca ggacggattg cctgaacaat atttggccct aatgtgatat ttgggacaaa
    16501 tcggccatcc attacatcta cgtggatgta atctgcgcct cctttttcca ctaactcaat
    16561 gtctctttgt aaattggcaa aatctgcact caaaatggat ggtgctatct tcattcttgt
    16621 ttcctcctat tttcgtttac cttctttttt tgcatacatt ggttttcttt tttcgatttc
    16681 ttgtaagaat tgcaaataat tgtcgtaacg tgtctgtgcg ataccacctg tttctactct
    16741 atgttttact tcgcagccag gttctttagc atgcatgcac tctctaaatt tgcaatggac
    16801 agaagcttct acaaattcag gaaattgtct cggcagttct cctgcttcaa ttgtcaaaaa
    16861 gtcaatcgag ctaaatcctg gtgtatctgc tactaacccg ccatagatag gaatcagttc
    16921 cacatgacgt gttgtatgcc gtcctcttcc aagtgcatca gaaatctctc ctgttgctaa
    16981 attcagttcc ggtgatattt gattcagtag cgtcgatttt cctgctccag actgacccat
    17041 aaagaccgat agccgttctg ggaagtaacg gatcagttct gtcgtatcac ccgtatgatt
    17101 gggtacaatc accggatagc ctattttagt ataggtttgc ttgatctctt cgattttttc
    17161 tgggtcgtct gacaagtcgg tctttgtcaa aaaaataatc ggttcgatcg actcgtattc
    17221 caaggtaact agaaagcggt caagtagatt ataagaaaat gcaggctcta cgatacttgt
    17281 gacaatgatt ccaagatcaa catttgtgac tggtgggcga actaattgat tttttctcgg
    17341 caagatttcc agaagatagc cgtctgtttg attttcactc tcaaagatca cctgatctcc
    17401 gacaagcggc gtgattttcc gattccggaa atttcctctt cctcttgttt gataggtctc
    17461 tccgtttgcg tatacatagt aaaaaccgct gattgctttt ctgatttgtc ctttgataag
    17521 tgccacctcc gtttttgttt attttaccat acgaagagga aagtaggtgt tattccatgc
    17581 aaacttctca aagtatatgg cttgaaaatt gatcaaaaga gatagaaaca caaaaaagag
    17641 aatgcaacaa atcaatgtcg cattcccttc tgatatattt tattcagcac ttattttgaa
    17701 ctttctgagg tgctagctga tgaacttgtg gagctggaag attcttcttt tgaagagttg
    17761 cttgtgctac tggagctact tgagctgcta gaactgtctg aagagtcttt ttcagaacta
    17821 gaagacgctg gatctggtcc tttagagtaa attacgttca caaccattcc tttggtaatt
    17881 gtcgttcctg cccctggtac tgtacgaatc actttatctt tatccaccgt gtttgaatat
    17941 tcggaagtct catgcccgat atattctgca ccgacactag ctaaataact ttgtgcttcg
    18001 ctttttgtat agccggagat atcattcaat ttaggctctg ttccttggct tacgacaaaa
    18061 ttgatgtaag tttcttctgc tacaacttca cccgaggctg gatcctgact gatgatcgaa
    18121 cctgatggta cagtgtcgct atagtcataa gtagcactaa tattattgat attgaatcct
    18181 tgtgtaacaa gccaattacg aacagtttcg tagttttctc ctacatgact actcaaagtc
    18241 actgtttctg gtcctttgct gactgtgaaa gtaatcgtgt ctgttttagg atcgacttct
    18301 gtatcttctg atggagattg cgaaatgatc attccgcttt ctacctctga actgaattcc
    18361 tcttcttttt gaatcaagtc agcagaaaac cctaattctt tcaaacttgc ttcggcatct
    18421 tcataacttt taccactgta atcatctaat ttgatttttt tcgtgccgct actaatatac
    18481 agagtgactt cgcgtttttg cttgactgtc gttcccgctg ccgggtctgt cttcactaca
    18541 ttaccttctt cgattttgtc atctgcaatt tcttctgttt ttgttgctac ttctaatttc
    18601 gcttttgcga gtgtttctct agcagaagct tcagaaagat cagaaacatc aggaacaaca
    18661 acttctcctc gcccgccaga agcaaagaat acagctgttc cgattcctaa aacagcaaga
    18721 atagctaata ggatcagcca ccattttttc tgtcctttct ttttaggatc aggcagaact
    18781 tcttcctcct ctggtaaggt ttcgttttct ggcttgtctt ttggcagagg catagatttg
    18841 aatgaatcag gcattggtgc atcttccgta aggggtgtga taacttttgt ttcattatcc
    18901 atcacatgag gctgccattt ttcttcgttt gcacgagctg gtgataatac tgtagaaaga
    18961 tcatcagaca tttcctcggc tgacttataa cggtctgcag gttctttagc cgttgcccgt
    19021 aaaatcacgt tttccagtgc ttgtggcaca tttggatcaa gtgcttttat agatggaaga
    19081 tcatcttgaa aatgttttaa agcaattgtt acagcagatt caccatcaaa cggtacgcta
    19141 cccgtcaaca tctcataaag gatgatccct aaagcataaa tatctgactg cttcgttgcc
    19201 atacttcctc tggcctgctc aggggacaga taatgaacag atcctagcat cgtattcgtc
    19261 tgcgtgatcg atgtttcaga taaagcgata gcaatcccga aatccgtgat ttttacaaca
    19321 ccttcattat cgatcagtac gttttggggt ttcaaatctc ggtggatgat ttgatggtta
    19381 tgagctaaag aaatagcaga aaggatctgc tgcataatat ttactgctgt ttcataagga
    19441 acaggataat gtgtttggat atatcgtttc aagtccattc ctttgacgta ttccatcacc
    19501 agatactgca tgttgtcttc ttcaccaaca tcgtaaacac taacaatgtt tggatgaacg
    19561 agttctgttg ctgctaaagc ttctctctga aatcgtcgaa tcgcagtctg gtcattttga
    19621 aagtcaaatc gtaagacttt aactgctaca tcacgatcca agattaaatc gtgcgccaaa
    19681 aatacatttg ccataccacc gctaccgata tttccagtga tttgataacg gccatttaat
    19741 tttttgccca actcgatcat acactgtcct ccttatattc gataagaaga acggtgatat
    19801 tatccgctcc gcctgcttca ttggcacgat caatcagctc agttacttta tgagacaatg
    19861 ggccttcttg attgatgatc gttcctattt cttcttcaga cagcatgttc gtcaagccat
    19921 ctgaacataa cagcagtcga tcttttagtt gccaaagatg agtagaaaca tctacttcga
    19981 ctgttccagg catgccgaca gaacgtgtta aaatattttt ccttggatgg ttcactgcca
    20041 tttcttcgct gatttctcct gatttgacta gttcattgac taaagaatga tcctccgtca
    20101 attgcatgat tttatcttca cgaatcaaat aagctctgct atccccaaca ttggcaatcg
    20161 tgaaccagtc atctgacaga gcggccgcta cgattgttgt tcccataccg ttatattccg
    20221 gctgttcttg tccacgctga taaatcagca tattttcttc ttgaatcgat tgaatgaacc
    20281 attgggcgat tttttcatcg tcaacagctt cttgtttttc ccaagcttcg cctagattcg
    20341 ttaccgccat ctgactggca atgtctccgg cacgatgacc acccatcccg tctgctaata
    20401 cagcaagctt gagacctgaa cggctggtaa atacactggc atagtcttga tttgtgttac
    20461 gtcgtcttcc tacatctgat tgatattcaa tctgcatttt ttcacctcat tactttttgc
    20521 gcaaacaaca aatgaaaaat ccatctgtgt aaaactgatg cggatacaaa gtcaacattt
    20581 tctcttcgat tgatgattgt agcataggat tggtcaaaac atcaatcctc tcaaaatcag
    20641 ggtgtctttc caagaaagca gaaacaacgt cttggttttc ttcttttaag atcgtacaag
    20701 tgctataagt cagaataccg gaagacttta aagttggtgc gcagctttct aaaatttcca
    20761 attggatttt tggtaaattc gctaattcat ccggctgttt tttataacga atatctggtt
    20821 ttcgccgaag cagcccaaga cctgaacaag gcgcgtctac taaaatccga tcaaactgct
    20881 ctgcttcgaa ctgtttactg acttctctag catccatctt ttgagcgtag acagcagaag
    20941 agacacctag acgtgctgca ttatcttcaa tgagtttgac tttgtgttcg tgtacatcta
    21001 aagcgaccac ttctccaccg catgacggat ctaaaaaaga agcaatatgc gtcgtcttac
    21061 ctccgggtgc tgcacaggca tctaagacat gatgttcagg ctcgatttgc atagacggcg
    21121 ctacaagcat cgaactttca tcttggatcg tcatgacgcc atcgtggaac aatgagctag
    21181 aagctaaatg cccttttttc gctactatac cgtatggact gacttggctt ttttcagctg
    21241 tgatctcttc ttcttgcaaa acagtgatgg cctgatctct ggaaagtcgg cgtgtatcta
    21301 ctcgggcact agcatggcta ggctgatata gcgataaacc gagcttttca gtttcttcgt
    21361 atccgatttg atccaccagt cgtttcgtca tccatagagg caggctgatt ttgacagaaa
    21421 gtctttcgat aggatcagca atgccttgca gatctggtgc tccttgacgc tgataattcc
    21481 tcaagacacc atttacaaat ttccctgtcc caggatttcc ttttactttt gcgatttcga
    21541 ccgcctcgtt taaaatagca tgtgcaggga ctttatctaa aaattctaat tggtatacag
    21601 aaagataaag aagcatcttg acccagtcat ccactttttt tgctttagcg atcaatggtg
    21661 caacgtaaaa ttccaatagt cgctgatggc taatcgtacc atagatcaat tctgtaaata
    21721 aacccacatc tttttgattt agctcgcttt gtttgatcat ctcattcaac aaaagattcg
    21781 agtaggcgcc acccttgttc actcgctcta atgtcagtaa tgccatatat cgtactgatt
    21841 ttttcatttt tttagggatt tttttcatta tttcacctgc tgaccaatct gaacttgctg
    21901 accgcttccg ttaaggaaat catgtacggc ctgttttcct ttaccagctg gctgtaattc
    21961 attgatggct aagacggtct gtttaccgca agcaatccat agattttttt tgtctttccg
    22021 gatgatcgta ccaggtgctt tgtctgtagt ttctgccaat acttcaaccg ataataattt
    22081 ccagcgtatg ccctcatagg tcgtgaatgc aattggccaa ggacgcattc cgcgtacttg
    22141 gttgtcaatt tcttctgcag ttttatgcca atcgattgct tcttgttccc gtgtgatgtt
    22201 tggcgaaaaa gttgcttctg actcgtcttg aggtactggt ttttgtccat ccaaaatatt
    22261 gggcaatgtt tctagcaata gctttcttcc taaaaggcta agcttttcaa acatcgtccc
    22321 tacatcatct tgtttagtga ttggtatgct ttcttgcgca tagattccgc ctgcatccat
    22381 ttttttgatc atttccatga tcgttacacc agtttctttc tccccattca tgatggcata
    22441 atgaacaggc gcacctcccc gatatttagg aagtaaagaa gcatggacat tgattgcccc
    22501 taattttggt acttgcaata acttttcagg caagaattgg ccaaatgccg cagtgacgat
    22561 cacatcaggc accaattcaa tgatttcttc catttctggc gaaccggaga ttttctctgg
    22621 ctgcagcaca cggataccgt gcttcagagc cgcttttttt accggtgtcg gcgtaattac
    22681 tcgtttccgc ccaactggac ggtcgggctg tgttaccact gcctggatct cgtaacctga
    22741 ttctatcagc ccttcaagaa tcggcacaga aaaagcaggt gtgcccataa atactagctt
    22801 agtcatcaag atgttcctcc atatattctt caagttcttc ttccgggatc tgctgtatca
    22861 ttttctcaat gaacaagata ccgtctaagt gatcgatttc atgctggaag gcacgggcta
    22921 aatagccaaa agccgtcact tctatctctt ctccgtctcg atcataataa cgcacggtca
    22981 cttcatctgc tcgcttgacc gttccataga catgcggaat actgagacaa ccttctacgt
    23041 caaggctttc accttttgct tcgatgattt ctgggttaat cagttcaaat ttctcgcctt
    23101 catctacttc gatcacagca attctcttgt tttgtcctac ttgaggagca gcgatcccta
    23161 ttccgtcatt cgcaatcatt gtctcatata aattgtctaa taaagcgatt gtctcgtccg
    23221 tgattacatc gattggctga gcagttcttt ttaatttatc attagggtgg atcaatatag
    23281 gatagcgcat aatttggccc tttcttaaat aaagtttaat ggttcgttgt caattgccac
    23341 aaaaagacct tgtcgctgtt ctttctggct tccattcaaa atttcatcca aaacatgagg
    23401 aagattcggt tcatgtttat atttgatgat tagttgataa taatatcggt ttttcactct
    23461 caaaatcatg ctgggcgttg gccctaatag caagctttga gggcttaaca cttcttttag
    23521 ctgattggca atctgaaaga ttttctttgc cgctacttgt tcttcaggat ggctggcggt
    23581 tattttcaca gtaaagtaat aaggaggata gcctccacga tgccgtacat gcatttcctg
    23641 ctgataaaac tgctcataat tttgtttttt agccaattct atggcgtaat gttgaggatt
    23701 gaatgtttgg atcacgactt cgcctgcttt ttccgctcgc cctgctcgtc ctgaaacttg
    23761 ggtaagcaat tggaatgtat gctcactaga acgaaaatcc ggtaaattca gggaggtatc
    23821 agcattcaac accccaacta atgtcacttc tggaaagtcc aaacctttag caatcatttg
    23881 tgtccctaat aatatatctg cttcttttgc accgaatttt tgtaaaatct gttcgtgtgc
    23941 cccttttctt cgagtggtat ctacatccat acgcaaaatc ctagcttgcg gataaagttc
    24001 tcggagttcc tcttctactt tttgagtacc tgttccatag tagcggattt tattgccgcc
    24061 acaattagga cagcgattgg gaataggttc ttcatgtccg caataatggc agcgcattga
    24121 acgtgtatcc atatgcagtg taagagagat atcgcaatta ggacatggaa gaacaaatcc
    24181 acagtcccta cacatcacaa aagacgaata tcctcgacga ttgagcagta ggacagtttg
    24241 ttcttttttg tctaatcgat tttgtatttt ttcctgcaaa ttagcagaaa aagtagaggt
    24301 ccgatgattc tgaaactctt cacgcatatc tacgatctca atagctggaa gctgtgcagc
    24361 tgctttggct cggtggttta actgcaataa ctgataaacg cctttttgag ctcgtgctct
    24421 tgattctaaa gagggcgtcg cgcttcctag aactattggg caatgatgat atttactgcg
    24481 ccaaattgct agatctctcg catgataacg cggtgtctca tcttgtttgt aacttgattc
    24541 atgttcttca tcgataatga tcagcccgat atttttcagc ggggcaaaaa tagcagatcg
    24601 tgcgcctact actacttgcg cttcttcccg ctcaatcttg cgccattcat catatttttc
    24661 accttgggaa agtccgctat gtaagaccgc aactgattct ccgaagcgtc ctttgaatcg
    24721 ctcgaccatt tgcggagtca atgcgatttc aggcactaac ataatagccg ttttattttc
    24781 tgacaaaaca tcagcgattg cctgaagata tacttctgtt ttcccgctgc cagtgattcc
    24841 ctccagcaaa aagacctgat ctttttgctg ctgacctgct gtgatgattt gttcgacagc
    24901 atttttttgt tctgcattca gttccaatgc cgtcgtttgg tcaaatactc ggtctttata
    24961 agggtcgcga taacgttctg attctataaa ctccagccag cccttttctt ttccttgatt
    25021 caagacagca gtactgattc ctttattttt gaaataagca attggttctt cttggctcag
    25081 ttcagataaa cacaaaagaa gttctttttg tttcttggca ttttgccgta attttgccca
    25141 ttcttcttcg atttgctttt cagttaacaa agaacggacc aaacgaatcg tttttacttt
    25201 atttcgtgta tggacttcat atttgatatc tactaattgc tcttggcgcc agcgaatcaa
    25261 ttgagaaagg ctgccttctt cttctgcttc cttccaggaa atcacttctc gctgatcaaa
    25321 caatacctga acttctggtg tgtctgccaa aggatagatc aatttatcat attctgcttt
    25381 catcacactc ggaagcatcg tttgtagaca agtgattttg aatgcgtaag taattttttt
    25441 catataatca gctaatgaaa gtaattcttc attgaccacc ggagcaaggt ctagcaaacg
    25501 aataactgcc ttcaaagatg gattagctga ttcttctgat ttttggatag ccataacaaa
    25561 tccttggaca tgacgattgc cattaccaaa agggacttct actcgcatcc cgacttgcaa
    25621 agctgtttcc atttcagagg gaaccaaata agtgaatggc tgatcggtct gcatagtagg
    25681 tacgtccaca atgacttcag ctgttaatgt catggttcgt ttcctccttt ttttgtcttt
    25741 tcttatcaag cgacaaaaag ttccttgcct attttactag agatcgatgg atttgtctta
    25801 tcttctgcca aatcctaaca aaaaattaac agcagataag aaaaaaaaga actgtgtcaa
    25861 aaagttcaga cacagccttt tctaccagac aataagtggc gaatgtttga aaaggaaaca
    25921 gctgggtttg cttttctttt tagatgttcg ctctattttt ctttattaca ttttttgctc
    25981 ttcacggata cgtgattcca attcgtgttg ttcacgctct ttttgtgctt gacgttcctc
    26041 ttcttccatc ttcaaacgtt cacgttttaa ttcaggatgt ggatcattga ctacatttcc
    26101 tgcatcgatt tcttctaaag cttgaccgac gctttttact gaatcaaaag aatctagagt
    26161 aggctgtgca cttgcatcca attcatgcgc gcgtttactt gctaaaatca ctagagaata
    26221 ttttgaattt acacgatcta atagtgagtc aattgaaggt tttaacatca taagactaca
    26281 tctcctttaa cattttaata tatttaccga ttacgcgatc tactcgaaaa tgttcactgg
    26341 caataatgtt tttgattcgt tcaactgcta gcggcacttc atcatttaca acagcatagt
    26401 cgtaaagagc catcatttcg atttcttctt ttgcaacttt cattcgtttt tcgattactt
    26461 catctgcatc tgttcctcgg cctacgatcc gtgattttaa ttccgctaaa tctggcggag
    26521 tcaaaaagat gaatacacca tcgggcactt tttctttgac ttgttttgct ccttggactt
    26581 caatctccaa gaaaacatct tttccttcat ccaatgtctt gttgacatat gacaaaggag
    26641 ttccataata attcccgaca tattctgcgt attccagcat ctctccagct tcgatcattg
    26701 attcaaactc ttctttgctt cggaagaaat aatcaacgcc ttcgacctcc ccttcacgca
    26761 ttttacgggt agtcattgag atcgaatact gaaaatcatt atcctcactc tcaaaaattg
    26821 cttttcgtac tgtgcctttt cctacaccgg atggaccaga caacacgatt aataatccac
    26881 gctctgacat gacgacgtcc tttcaactag tctttttgta cttatcgttt attttgcact
    26941 ttttttctgc aactttcaag gttatcttta attatagcta gaaattggta aaaaagctac
    27001 cctgtaccaa gaggtcgtaa aaaagctgtc ctgcatcaag taataagaac agccaaacat
    27061 aaacagcttc tcatgttttt cgtttggttg gggttattat cgcagatgca agcttttgaa
    27121 cgccgtgtat aagaggtcgt gaagaagccg attagtttcg agtatgaaga aaaattttgt
    27181 gaaaatagtt tctcacattt ttgcataaat ttggcttaat accgagaaaa tggtttttga
    27241 acatcactta ttcaaaataa aaaaagattg tgacacgaat tttgtcacaa cctttaaatg
    27301 tattcctaga gcaaagaagc taagcgaaga aatccaattc tgtttattta ttgtatttct
    27361 ttgttcagca tccttgctaa aagcccatat tggacaaagt attcactaca cccatgatct
    27421 catcttttcg agaacgtgct ttttctgtcg gatcttctac ctcttctgtg tcaattcgaa
    27481 caataaattt ccgattcatc gtcacgtaag caccgtcgtt gatcaaaata aacagttcgt
    27541 tctcattata gtctgcacag gcatctttcc atacgtcaaa gccgtttttt tcagaaggga
    27601 tatcccgttt aaccacagtt tttccactta attcgtggaa cgttacgtca taatatttca
    27661 ttatctctac ctctttcgtc attcaatatt ttgtacttgt tctctgattt tttctaagat
    27721 cgtcttagac tggacgactt gatttttaat ttcgattgca tttgatttgg aaccgatcgt
    27781 gttgatttcc cgattcattt cttggatcag gaaatctaat tcccgaccta caggacattg
    27841 tgcttcgata agttcatcta gtttttgtac atggatcgcc agacgatcca gttcctcgtg
    27901 aatatctccg cgttccaaca agatggctag ctccgtcaac aaccgttgct catcgacttt
    27961 cgctccaaga aattcctcca gctttttttg ataccttatt tgaaattcct tttcatagat
    28021 ttctacaaaa ctatcgagtt tttttagtac ttctttgaat tcttcagtat tctttttcaa
    28081 cactaaagct aaagcttgac cttctttttc tcgactcgcc tcaatagaat caagtgcgct
    28141 tttcaatgta tctagagcta actgtgccaa ttcttccatg ctctcttccg gtttttcttc
    28201 taccgttacg aagttttcgt ccagcacgaa gcgttccgcc atccgactaa ttgacacttg
    28261 attttgacta ccgtatcgtt gttgaattcc gtctgtcaat tgagtgataa gtgaatcaat
    28321 caattcccag ttgatgacga tctgtttttc gtttttgcca atataagcta gattcacaaa
    28381 tacttcgatt cgaccgcggc ttaactgctg tttaataagc tgacgaaaat cgttttcaac
    28441 aaagttcagg attttaggac ttcgtatttg aacatctaga aaacgctggt tgacactttt
    28501 tatttcaata tctaactgat acgcttttgt ttcgcggatg gctttgccaa aaccagtcat
    28561 acttttcatt cgacagggtc cttcctcatt acatattttc tattgctttt tgcagttgtt
    28621 caaactcttc gttcgtcaat gtcaggcctt tccccatttt ttcatggtct ggcgcccaat
    28681 ctcgaagatc gaattttggc gggcgaccat tccagctgac taggttcagt tctttacgcc
    28741 accccttgtt gttttctgac aatatagcga tttcttctac gatttcatat gaaaattctt
    28801 gtgccatggt tgttcctcct tgagataata taaatatagg atcagtaatg tgtcaactgg
    28861 ttgcttaagc aattcatgct gcaaacgatc cttgcgtttt ccttgtagca gataatcaaa
    28921 ataaacagtt ttcgtgcgct cattttgtat tttttgctgg tattttcgat accttcgcca
    28981 ttcttcagta cttaattgac caatttgtgc caataaatca tccggcatac tttttatatc
    29041 ttgttggcag atcatttgaa tcatcatgtc tacttgatag atttcattgg gcagtaaaaa
    29101 atgttttttt gctaaaagtt ctagcatcgc caatccgcat gtctctggta cgaccctgtt
    29161 cttatataaa ttacgcagtt tttgccaaaa ttttatgctt ttgaaaaaag ataattctaa
    29221 ctgaatttct tttgttaagt aagacaaaaa tcctcgccag tatgttcttg caggtttaac
    29281 agaatgaaat gtataccagt tcccatagta atagcctaat cgttttttca tttccagata
    29341 gcctaattgt aaatttaatt ggttcctttg gctatcaaaa agcagaaagc tgcctagtgt
    29401 ccacaatgtt tgacatttcc aatggataaa aatgtctggc atacgaattt ttttcgctgg
    29461 gcctggccct tgcacatcta cgacaaatgc ctctgttgct cctttgttaa tcgcaatatc
    29521 aataggtatt ttgttgcgat acccaccgtc tgcatattta ttttttccga tctttcgata
    29581 agccatagcg ggataaaatg aagcagaggc gagtatccaa tctggtattt cttctgtttt
    29641 tgtctgttgg atatcagtca ctacttccgc tagttctggc agtttagtag acaccgtata
    29701 taaacgtatt ttgggattat gtttgagtaa ggacaaatcc aaattgtctt tgaccatctt
    29761 ttccaatgga gcagaagaaa ccccgccttc aattagtgct gttttcgtca tttggcgtct
    29821 ttctcgatag aagcgttcac gaaggtcttc aactgcagcc atttctggta acgcaagcac
    29881 ttgtcttgtc gacagctttt tccataaatt cacagctttt ttcatatctc cttgtaaaat
    29941 caatgcgccg ttcaacgcac ctaccgaggt acctgtgata atctcaaagt tgatatcatg
    30001 ttccttcagc gcctgccaca caccaatctg ataagcaccg tgcgctcctc ctccacctaa
    30061 aacaagtgct gtatgataga ccagcttttt atttaattgt gtggttcctt gatagccgtt
    30121 tttatgaaaa atacgcaacc attcttctga tagaggagat tgaaataaaa aacaacagtt
    30181 ttctttaata aaaaaacgag cgcaagcttc catataccga aaaaattttt tccattcaaa
    30241 agtttcacct tcaatcaaca gattattaaa aataacatct cctctacttt tagcaatagt
    30301 aaaatagacg gtatattttt tcgatactgc tttaaaagca cattgctttt ttgcagaacg
    30361 ccaaaaagcg tctctacttt cttgatgact ttgaaaaaat ggaaggggtt gttttgcttg
    30421 ctggataggc gaatacagcg acattttctg tgaaaagtaa acttttttta tctccatcgc
    30481 gctctccttt ttcttcaagt ataccagtac gtcataaaaa ggtcatgagt ctcgtattcg
    30541 actttgtaga ttccataaga aaacaattca taaaacctcc tttaaataaa aaaatacaaa
    30601 aatcacaaaa attaattgaa aatgagagtg ttttcaatga aaacgtgatt ttgtaatgga
    30661 attttaaaat aaaagaaaca agctttttta atgcaaatgt tgtaaaataa aagagattaa
    30721 gaggaaataa gaggaggaac atgatgactg agtcagttta tgtacatatt gataccacaa
    30781 gtaatgctgt tttaacaaaa gggctgactg ctactgactt tgcaaacagt atcgtgcatt
    30841 ttcctcaaaa cttgctcttg cttgatccat ctgcttctgc tggagaatat gaatcccata
    30901 cagggctgaa agtaatccgt ggtacagaaa atattcagcg tttcttttcc tcttctcgta
    30961 acaggcgtgg attcgatgaa ttgaagtgga tcgactttac tgatttaacg atgctgaaag
    31021 aattgacacc tttagaaatt tcagaattat tatattttgg tcatatgaaa acacatcttc
    31081 attcgccgtt tttttataaa ctacagaata attttgtata cttcgatctt aatgatcagc
    31141 tgaatcggat ttattatcgc tatttagatg aattttatag gatttttgct agtaaattaa
    31201 gttcgatctt gtcagataag atcaatgtaa aaaaatcttt tttcaaaaaa gaaacacctg
    31261 ttgaaaaaat cagtgtggaa ttattgaaag aaatgcgtaa tttgatgcag gaagggatca
    31321 ttttttcttt tgatcaagtt ggtttgatcg atggtaaata tcgtatccca atctatgtga
    31381 ttgaagatta tatttggaaa ttaaaaggca cccggtatcg agaagaggaa atcatcgcta
    31441 ctttaatcta ctctgtcacc gatcgacact ggcaattggt agaagatgcc gatctttctt
    31501 atctgccatt gaagtagatc tatttcttat caaaaaataa aaaggaaaca agaaactagg
    31561 acatgattct gtctcaagtt tcttgcttct tttttattta cagctcctgt cgtttatctg
    31621 gcacaaaaat cgaaggagtc gtttgttgga tccctaataa aacgatgatc gttacaatag
    31681 ctgtactcaa cccgctggca ccattcatta cgaaagaaaa gagccaagga ttcatccccc
    31741 atagtgcata cgctccccag aaaaccactc ctgcaataaa atgccaaaaa tatctggaaa
    31801 gtgcgccaat caatgcacca aacacgatgc tttttctaat ttgtttcgta ttatcgtcta
    31861 catagccttt ttggacattt gaagccatga gccctgctgc gcctgcaaaa gtataggcaa
    31921 tcgggtactc aatcaacact tgaacgacac ttaaataata aacttgtccc aacggaaaat
    31981 gtaggatgcc ccaaatgaaa cctgccagca tccctgcctt tgtccctctt cttaacgcaa
    32041 aaagagtcaa tggaatctgc cctaatgaaa tagaaaaact gctgccgatc tggatcggaa
    32101 tcaacgacaa caccatcgct aacgctgcaa caatcgttcc ttctacccaa actctatttt
    32161 ttcccataaa aaaacttcct cctttgaata aaaaagtcac aaaggaggaa gcagctttcg
    32221 aaaatagtca aacagctgtt tattcacaat tcctacgctc gcataaccga tacaggtacg
    32281 aagggtttag aaatagattc aatctcagcc caattggact cccctttgtg agtttattta
    32341 ttatttattt tttgtttcaa acaatggact aactggtttg ttttcatgga tacgtttgat
    32401 tgcatctccc attagtccgc caacacttac ttcatcgatt ttgtcgattt tacgatcttc
    32461 tggaagatag atcgagtctg taacaactag acgctcgata gctgaatctt ctattctttg
    32521 taaagcaggg ccggataata ctggatgtgt acatgaagca tacacactag ttgcaccagc
    32581 ttctttcaaa gcattggcag ccaatgtgat cgtacctgct gtatcgatca tatcatcgat
    32641 cagtacacaa gttttgcctt tgacttgtcc aatgatattc attacttctg caacatttgc
    32701 ttttggacgg cgtttgtcta tgatcgcaat cggagatttc aaaaattccg ctaatttacg
    32761 tgcccgtgtg acaccaccat ggtctggtga aacaacgaca acatcatcgc ctttgattcc
    32821 tttttctaag aaataattgg caataagtgg cgcacccatc aaatgatcga ctggaatatc
    32881 aaagaaacct tggatttgga ctgcatgaag gtccaaagtc agcattcttg ttgcaccagc
    32941 tttttgaatc atgtttgcta ctaatttcgc tgtgattggt tcccgtgcac gtgctttacg
    33001 gtcttgacgc gcatagccat aataaggcat gactacgttg attgtttttg cacttgctcg
    33061 tttcagtgcg tcaatcatga tcagcaactc catcaagtta tcattgactg ggctgctcgt
    33121 tgattgaata acatagacat gcgatccacg aatactttct tcaatgttta cttgaatttc
    33181 tccatcacta aattgagtaa cagatgattt tcctaactcg acgccgactg ctgctgcaat
    33241 tttctctgct aatgggcgat tggaatttaa cgcaaaaatt tttaatcttg ggtcaaagta
    33301 atgttttgac atggggacct ccactttctt tttcacaact ctagaattct acctaataaa
    33361 tagtagtcat attttttaaa taaatcaagt gattttgcaa aagtcttagt ttagataagg
    33421 gagttttttt gcatatcctt ctttattgac ttgtcttgct ctcgcaattg ccagcgcata
    33481 ttctggaata ttgtctgtaa ttgttgaccc ggcagcaatc gaactgtttt tagctacctc
    33541 gacaggtccg atgatattcg tgctagaacc aataaagctg tggtcgccga cagtcgtatg
    33601 atgtttgttc tttccatcat agttgacgaa gacaacacca catccgacat tgatttcttc
    33661 tcccagcgta gcatcaccaa cataagtcaa gtgaccgact ttagttcctt tgccgatttg
    33721 agcatttttc acttccacaa agtttccgat atggacgttt tcgccaattt cagcttttgg
    33781 tcgtaaatgt gcataaggtc ccacatctgc atgtttcttg acatggctgc tttcaatgac
    33841 tgagttttca atgacgacgt gatcctcaat cctactgtct acgatttttg aatgtgaccc
    33901 gatgacacaa tcagaaccaa tgactgtatt tccttggatt tgtacacctg cttcgatcaa
    33961 tgtgtctgga ccgatttcta cgcctgcatc gatgtatgtc gttgctgaat cgacaaagct
    34021 tacgccattg accatatgca tctggttaat tcgctttctc atgatctcat ttgctttagc
    34081 taacgcgatc cgatcattga ctcccattga ttcttcaaaa tcatccgttt gataagcagc
    34141 aactgtatgg ccttcttctt ttaagatttc gatgatatct gttagatagt attcaccttg
    34201 agcattgttt gttccaactt tgcttaacgc atcgaacaaa gcttcgttgt caaaacagta
    34261 agttcctgta ttgatttctt gtacaagagc ttcttctggt gttgcatctt tttgttccac
    34321 gattttttct acgatcccaa tatggtcacg gatgattcgt ccatatcctg ttgggttttc
    34381 tgcttgtgcc gtcaagatcg ttgcacttgc attttttcct tggtgatatt caaaaagatt
    34441 gttcagcgtt tcagtagtca ataatggtgt atcgccactg ataactaacg ttgttccttt
    34501 ttttccttcc aagaaagaag cagcttgcaa aacagcatgt cctgttccca attgttctgc
    34561 ttgtaacgca tatttactgc gttctcctag ttgtgcttta acttgttctg ctccgtgtcc
    34621 aacgatcgta atcacttcat ctggtttcgt ttcactgacc cgattaatga tatgttctac
    34681 cataggttta ccagaaacgg gatgtaaaac tttatataat tttgacttca tgcgggtacc
    34741 tttccctgcc gccaaaataa ttgcataacg tgcgtccaac tgcgtcactc ctaaatatag
    34801 tttgctgatt tcagtttatc ccagtatacc cattttttca acaaaacaag tgattttctt
    34861 ttatcccagc gttttctgga taaaaaaata gatagaccat aacgggccta tctatttggt
    34921 aaccggtgct ctcctgccaa ctcctcggtc acaagtcaca ctactcataa aacatgaaaa
    34981 gctgtttttt gaaatcgttt cttgtgactt agagttaaac ggtaggctta caacctcttc
    35041 attaaacgtt tttcaatcag ctgaccttcg gtattagctc aataaacggc aaaatatgag
    35101 gagcaatttt tccgtttatt gagctaatac tcaggacagg acgctgattt cacaacctct
    35161 agataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg aaaaaatgtg
    35221 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcggcttt ttcacaacct
    35281 cttattctga aaaataattt cctggcacaa ccgtaatatt tttcgtttga gtatctacat
    35341 ctttgacata aagcagtgac gtataatcat cgatggcgcg atgaccgttg aattttgatt
    35401 cggcaaatac agtgatgcca acaagttctg attcgaattc ttcaatcaag ctcttcattc
    35461 cgttgactgt tccgccgcct ttcatgaaat catccactac aaggacacgt gaaccacgtt
    35521 tcaaacttct cttagacagt tccatctttt caattctttc agaagatcca gacacatagt
    35581 tgacacttac tgttgatcct tctgttattt ttgagtcccg gcgtacaata acgaatggcg
    35641 cattcagata ataggacaca gcttgggcaa taggtacgcc ttttgtggct acagtcatta
    35701 cagcatcgat tttttcacca aggtatttag aagcaatgat tcttccaact tgacgcagca
    35761 gttcaggttc tcctaacaaa tcagagagat aaacatatcc tcctggaagc agacggtctt
    35821 gttctgatag acgttctgct agagattcaa tatatttttt cgcctcttca tatgaaattt
    35881 cagggatgaa caatacacca cctgcagcac ccgggattgt ttccaaggtt ccataccctc
    35941 gttctttaaa tgttttcttg atgattccca aatcttcact gatagaagat ttagcagatt
    36001 cataacgttc ggcaaagctt gttaaggaaa tcaattcatg cgggtgattc aataaatact
    36061 gtgtcatgtc aactagacgt tcactgcgac gtactttcac ctattccaac tccttttaag
    36121 aaattttatc gttttacatt atataagttt ttcttcaaaa attcgagcat tttatgaaaa
    36181 aataagaaaa tagttcggta tttattaaaa taaaaagaag cctataaaaa atttttcgtt
    36241 tttataaggc ttcttttctt tataatttag ttattttttc ttctgaaggc aaccaatatt
    36301 cgtttgatga tatttacaat caggaacaaa gcaataaaga ctaatgtgat tgttgctcct
    36361 ggaggtgtat ctagataaaa cgagctagtt agtcctgaca gcatccctag aaatcctaca
    36421 caaacactga tagcaatcac gacattgaag cttttgccta atcgcatacc gattgctgcg
    36481 ggaagaacca tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat cacagcaata
    36541 gccacaccag taatcacatt gaacatcatc gacatcaaat gaacgggtag cccatccacg
    36601 tgggcggtat cttcatcaaa agtcagaaca tacatcggcc gtttgaataa aaagaacaat
    36661 ataccgatgg caacgaataa gattcctaaa aagatgactt gctcccatgt aatggtcacg
    36721 atcgaaccaa acagatagga ttggatactt gttgctgaat tccctccgct caaattcatc
    36781 aatactaacg ctagcgccaa accaccagcc attaaaatgg caatggagat ttctgaatag
    36841 gttcggtaga tcgttcgcag atactccaat atcacagctg ctaatacaac gactagcatg
    36901 gtcgtaagtg taggattaag gttcaaaaag aatcctaacg ctacccctgc taaagagaca
    36961 tgagaaagcg tatcagccat cagcgattgt cttctgatca ctaaaaacac acctagcatc
    37021 ggggcgattc cggcaatgaa gatagcggca agaaacgctc ttctcatgaa ttcatatgaa
    37081 agcaacgcca ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc gcatactgtt
    37141 tgatatcttc atgatcatgg gtgatcatta gaactgattt cccatggaca tgcgcactat
    37201 gtcggagaag acgataaaat tcatttctgg attcttcatc catacctgtt gttggttcat
    37261 caagaacaaa taagtcaggg tcagtagcaa aaatacgcgc taaactgatt cgctgctttt
    37321 gtccgccaga caattcacct attcgccgat ttcgcatatc ccacatccct acagcttcta
    37381 aagcacgctg tacatgttga tgatcttttt cagagagacg cttgaaccag cggcctcttg
    37441 gatatcttcc tgaacggact aattcgatca ccgtactagg aaaacctgca ttgaaagatg
    37501 caacttgttg aggaatataa ccgatactta atttttttcc ttcttgattt ttctcagcaa
    37561 tctttatcat tccttttgaa ggtttcagta atcctaacga tgctttcacc aaggttgact
    37621 tggctgctcc attttctcca gttaaaatga caaattcacc cgcatctaca tgataagaga
    37681 catcttctag tacaggttca tcatcatagt aaaaggtcaa atcagatatt tcgatataac
    37741 gcaccttaca acctccattc taaaacttaa ttgatacttt cttttaaagc tttcagattt
    37801 ttttccatga cagaaatata attttcgcct gctttgattt gttcattagt caagctttct
    37861 aaaggattca acacttctag cttcactcct gtttcattcg acaatgtctc agctaccttc
    37921 gaagaggcat tttcttcaaa ataaatcact ttgatgttgt ttttcttcac aaattctttt
    37981 agctcagcta agcgacttgg tgtgggttcc tgatctggtg aaacgcccga aatcgctact
    38041 tgttttaagt cgtattgatt cgctaaataa gcaaaagcag catgctgagt gacaaatgtg
    38101 cggtttgttg catcttttag ctcatcggag tatttttgat ccagtgcttc aagctttttg
    38161 atataagcat cggcattctt cgtaaaagtt tcctttttat ctggatattt ttcgcctaat
    38221 tcttcagcaa tcgttctgac ttcttttata gctaataccg gatcgagcca aacatgtgga
    38281 tcgtattcat gtgaatgacc atgcccttct tctgattctt catgttcgtc tgcttcttgc
    38341 gattctaaac ggtcgattcc tttagctgct tcgatcatca aggtctgttt gctatccact
    38401 gaatccttta acgaatccac aaaaaattcc atgtctgaac tattatacac gaaaacatct
    38461 gcatcggaaa tttctgctct ttcttttgca cttggttcga aatcatgcgg ctccgttcca
    38521 gcagggatca aaagtttcac gtttccttca tcccctacga tctcttttgt aaaatcatac
    38581 atcggataaa aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta
    38641 tttccacatg ctgcaaacag taaagcacct atcattaccg tagctgtcaa aagatatttt
    38701 ttcatttata tgctcctttc aatgaacaaa tcggaatttt acgatttatt aacgtgaact
    38761 aatgtatcaa aataaaaaaa cacagtcaat agaaatccat caatttctga tttaagcatg
    38821 tatattcttg attaaatcgt aaaatggatg agttgctgct catatctagc catttcacta
    38881 caataaaaaa ctgaagaata ccattttagg attcctcagt ttttcatcac ctattttact
    38941 tcttatattt tattgcaggg ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc
    39001 attatatact ctctgcgctc ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct
    39061 tccactcatc aatgcagcat ctgctccgta cttgatcata cgctctttga tttgatggat
    39121 gactggatgc cgctttcccg ttatatcttc taaactgttt cccatttttt tgatcatctg
    39181 ctgataatct tgctctcgga tggcttcagc aagtccgtgg atgtcttgat gttggatggc
    39241 ttgcgtatct acttctctaa acactgtacg tgtagagaca ctcattcttg gtttgaccaa
    39301 cacaacccag cactgtggca tagaaggtaa tacttggatt tcttcccctt tgccaccgac
    39361 aaatgccgta cttccgtaga cacagtaagg aacatctgta ccgacttcca aaccgatttc
    39421 tgccagttct tctaacgaac agcccagatt ccataattgg ttgatccctc ttagagcagc
    39481 tgcacagtct gtacttcctc cgcctaatcc agctgctact ggaatattct tcttaacaaa
    39541 gatcttgaca ccggttttga tattgtattt attttttagc aataaagcag cttgataaac
    39601 attgttccgt tcatctaccg gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc
    39661 tgttatttct tcaaaaaata gatgatctgc caagtctacg ctcgacatca ccatttccaa
    39721 ctcatgatac ccatcttgcc gtctatataa tacatccagt cctaaattta tcttggctgg
    39781 cgctttttca atcaattcca tcctctcacc cattcgaaaa gtctaagtcg caagtattct
    39841 atcataggga aaaataaaag tatagcttat gtctgcctag tggttgattt tgtcatcgtt
    39901 tcaacagctt tcacagaatt ttctcttcat aatccaaaca agttctagtg attcttataa
    39961 ttctgtgagc acttcttctg ccaaactcgg aacatttcct ttttcttttc ctatcagact
    40021 gagtagctgc atggtagcaa atccgaaatt acttcctttc ctataagcaa aataacgtcc
    40081 ttcccaatga tcgacatatt ttaatttctt ttcataagca gactggaagg aatagaaaaa
    40141 attaccataa ttatatacga tatgcatgac tttttcttga aagaaagaaa aatctgtttc
    40201 tccaacatta gctaatggag ctaagcctag attggcttcc gagtagccca ttttctcgta
    40261 tacaataaac agattggcta acagatagtc agctaattcc tcagattctt taggtaagac
    40321 ccgcaacaaa tcatatcccg cctgcttctt cgtcatcggc tgagcggtga tgaagccaac
    40381 gactttttga tctttacgga caataccgac accgctagat aatacatatg atttatcaaa
    40441 tcgtcccaca gagaagtatt tttctcgtgc accttgaagc catttttgtg aaacttcatt
    40501 tacagcttga tgtaattctt ccggcagttg atcgtaccaa gtaaaatggt atccttcttt
    40561 catcaaacgt tgatattcaa gttgctgact cagccgagac aagtcttctg ttgctagatc
    40621 aacaatccct gcttcaccta ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac
    40681 gtagctttct gatatcttat aaaacgcaag ctgatagcct aaacaatctg cttgtttcat
    40741 aaactgattc gttgctgccc gcatcatttt aggattgcca actggatctc ctaaaacaaa
    40801 gcatttgttt cctttgatct gataaccaaa aacgacttga tcttggtcgt cttcttggta
    40861 ataaaaataa ctgtaacctg acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt
    40921 ttccaataaa cggtcaaaac ggtctttttc gtactcactg ccaagcttcg tagtatcttc
    40981 agctaaatat tgataaagcg ccactaaagc tagcaatgag atgcccaaac caataagccc
    41041 tgagaaccac acatcatctg aaggaaacaa aataaaatga tttcctgtta ttcgaccgac
    41101 taaaaagcgt ccgctgtgat agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa
    41161 gctagcatca aagattatag ctccccaaga ataaacgaat tttgttcgat aaaattcttt
    41221 tctggataac cacacagcta acagaatcaa gacatatacc aatatcaact gccaagataa
    41281 cgtacgtgtg attgtattta aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc
    41341 ctttttgact tttactgaaa gtgctctagc aaggccgagt aataaaaagc caaccaatag
    41401 attcactgtc tgatctaaaa aattgaatga gaacggcaat aatacttgaa atagccgact
    41461 gattgcagac agattagtga ttgttgatag aagtaccatc atgatccccg caaaatagac
    41521 agcacttacg agcacgatat gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa
    41581 aaatcgattg attttgactc ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg
    41641 tagaacataa taaaacaacc gataatataa tagccaaact actgctacat cttgccgaac
    41701 agacaattga ctcattccta agatcatcaa aacgtcaaaa gtccccattc cacctgggac
    41761 catcgtcagc attcctatca atgtagcaat catgaacata ggatagatcg aaagcatcga
    41821 aatatctatt ctcatcaact tgccgatgat caaaaatacc gcaagggctc ccgtccattg
    41881 tccaaaggat gccagtagca gaccaagcaa gccttttgga taaaactcga agaacaatgt
    41941 ctttctcttt agataagcaa aaaagaaaat gactggtgct aaaaaactac cgccgaaaag
    42001 ccagatccaa tagcttcgaa aaatactttc tgactgaata aaaaagacat caataaatgt
    42061 aagaaacgac catacagaca aaccagtcag cataaatagt gcgacttttg atacagtggc
    42121 taaaaccatt tttctatttg tctgctttcc ataaaaacta gcccgcagac ttgctcctac
    42181 cacaccacca aaaccggcga gattgttgat cgtgtttgtt acccacgcag caagaagaaa
    42241 ctcttttctt tccatttttg gttttccttg tttttctaag actctgatcg tcacccaatc
    42301 atacaacaac atcggagtga cacccagcag tccagccaga atcatgagca ggatcgtcat
    42361 gcggctttgt tgtcccatcc tttgccaaat atcttgccaa ctcatgccat tagcaatatt
    42421 tgcgacttga tttgccacaa agactaacac ggagccgaaa aaaatcagtt ttaaaatcag
    42481 actatgttcc tttatccatt gcagcatcaa gtatcgttta tttttcacat tttcccattc
    42541 ctaacttgta tacttttgtg tcatacccca atgttatctt atttatttta atttttccac
    42601 tttagacctg ttctttgctt gcgatgatag aaaaagaaag atatcctaat gctaaaggag
    42661 aggataacat gaaagtagga tttatcggca cagggaatat ggcacaagct attattgatg
    42721 gtttattaaa aaaaggaaca gtctcgaaag aagaggtgtt cgtttccagt ggacattttg
    42781 aaaatgcaca aaaatttgct gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag
    42841 ttgcatcaca atcagatatc gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat
    42901 tggaagaact aagagaatat agccagaaag tcttattcat ttctattgct tcaggaaaat
    42961 cattaataga tttagaaaca gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc
    43021 cgaatgtcaa tgtcagcgta ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca
    43081 cggaggaaca gcttcagcaa gctacgatga tttttgaagc aatcggcagc gtctatcctt
    43141 tagcagaatc agatttcagt acattcattg gccttgctgg cagctcaccc gcattcgttt
    43201 acatgctgat cgatgcgatg ggacggactg gtgtactgaa tggtttgtcc aaacagacag
    43261 ccactgagat tgctgcaaaa gcaattatgg gttcttgtca aaaattcctt tcaagtgata
    43321 aaaatccatg ggaacttgta gaccaagttt cttctcctgg cggtacaact gttgcaggtg
    43381 tagttgcctt agaagaagca ggattcatcc cagcaatcat caaaggaatc aatacgacga
    43441 tcgagaaaga tcttgagatg ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt
    43501 acaaaaaaag aagagctgtg gcttttgccg cggctcttct tttgtatttt ttattattta
    43561 gcataatcag ttgcacgagt ttcccgaata acgattactt tgatgtgtcc aggataatcc
    43621 agttcttctt cgattcgttt acgaatatca cgtactaaac gaacagcgtc taaatccgag
    43681 atttcttctg gcttgaccat tacccgaact tcgcgtccag cttgaactgc gtaacttgat
    43741 tctacaccgg cgaagctgtt cgaaatattt tccagacttc ttaaacgacg gatgtagttt
    43801 tctaatgatt cacttcgcgc accaggtctt gctgctgaga gcgcatctgc tgcagctaca
    43861 aggacagaga taacagaagt agcttccaca tctccatgat gagaagcgat cgcgttgatt
    43921 actactgggt tttctttgta cttggcagct aattccgcac cgatctcaac gtgagatcct
    43981 tcaatctcat ggtcaagtgc tttcccaata tcatggagca acccggcacg ttttgctaat
    44041 tggatatctt cgccaagctc tgctgctaag ataccagcta attttgcaac ctcgaccgaa
    44101 tgcttcaaga cgttttgtcc ataactagtt ctgaatcgca gacgtccgat gatcttgatt
    44161 agatctggat gaagtgtatg agcaccgact tcaaacgctg cctcttcccc gtattcgcga
    44221 atacgttcat ccatttcttt acgagatttc tcgaccattt cttcgatacg cgctggatgg
    44281 attcgtccat cttgtatcag tttttccaag gtcatccgtg cgatttctct tcggattgga
    44341 tcgaatccgg aaagaacaac tgcttccggt gtatcatcga tgattaaatc gataccagtc
    44401 agcgtctcaa gcgttcgaat attacgacct tctcgtccaa tgatacgacc tttcatttca
    44461 tcattcggaa gggtaacgac tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt
    44521 tgaatcgcta atgataataa gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt
    44581 tcagattctt ttaccattag agttaactca tgattcagct cttcttcagt cgatttcatg
    44641 atgacatctc gtgcgtcctc ttttgataaa ccagcaattt tttctagttc tttttgttga
    44701 tcttcaatca ttttttcaac gtctttttct cgctcttcaa ttaattgctg ttttgaacta
    44761 agtgcttctt ctttactttc aagtgagttc tcgcgttttt ctaacgagtc atctttacga
    44821 ttcagcgttt gttcgcgttg aatcaaacga ttctcttgag attttaattc taacttactt
    44881 tctctcagct cactttcaac ttcagaacgg tatttctgat tttcttcctt agcttctaac
    44941 aatgcttctt tttttagagt ttctgcttct ttttttgcac tatcgatgat tccagcagct
    45001 gatgattgtg caccagctat ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa
    45061 ccaagaccga caattaaacc gatgatagcg aggagaatgt tgaataccat aaaatccacc
    45121 tccatactat ctttttcatt tttatcatct atacagcttc tatgataaat tattctaaac
    45181 tcgtcaatat gcatcttcgc atatatgtgt acaaaaaaca tgcacaactt tattctaatg
    45241 tttgtacccc gtagtgtcaa ctttaaataa acaaagaaac ggctttctac ttattcatta
    45301 aacgatggtt tttaaaataa atgacactag tttcatatag gtaaaccttt aaattgagaa
    45361 acttttctat taaattattc tttgtagatg aacagatatt tttattttat gtacccactc
    45421 gtataattaa ataactcacg atttctttat tcaataaaaa aacggcaggt caaggagagt
    45481 acctactctt cagaccagcc gtttttgttt atcataattt ttattcttct actaaaggca
    45541 attcttcttg tcctttttca tcttctggga cagcgacttc ttcaccaata ccataagctg
    45601 ctctaactag agcagaaact tctgccatca tttcaggatg attagccata tagattttag
    45661 cgttttcccg tccttgacca atccgatctt ctttataaga ataccaagca ccgcttttat
    45721 caacaatgtc tttttctact gccatgtcta gaagttctcc ttcttgtgag atccctaaac
    45781 catacatgac atctacttca gcgattttga aaggaggtgc tactttgttt tttacgactt
    45841 tgatctttgt acggttacca acaatatctg taccttgttt caattgttct gcacgacgta
    45901 cttccaaacg gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg atttctggat
    45961 tcccaaacat cacgcctact ttttcacgga tttggttgat gaaaatagcg attgtttttg
    46021 ttttgttgat cgaaccagag agtttacgca atgcttgaga catcaaacgt gcttgtaacc
    46081 cgacatgtga gtcacccatt tcgccgtcga tttctgctcg tggaactaaa gcagcaactg
    46141 aatcgactac tacgatatct acagccccac ttgatactaa agcatcagcg atctctagac
    46201 cttgttctcc tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca cctaattttt
    46261 gtgcatattg cggatctaac gcatgctcag catcaatgaa agcggccgtt ccgccatttt
    46321 tttgtacttc tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat
    46381 atacttcgat gatacgtcca cgggggtaac cgccgacacc taaagcgaca tctaacgcaa
    46441 gagagccact tggaattgta gagatttgtt ggtcgatttt ttcgcccaat ttcataatag
    46501 agcctttacc atagctcttt tcaatttttt tcagtgcagc atctaaggct gctttacgat
    46561 catctgccaa tacgataatc ctccttatat ttacaaatca gagcaacatt tcattatcct
    46621 tatcatatct tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc
    46681 attttcttaa aagctgtttt ctcaaaagat caaatccttg cataaccgca ttatatctga
    46741 tagcagcacg atctctggca aagtgatatt tttccgaaac cacgccgtct ttagatgaaa
    46801 tagcgatcca tactgttcct gcagattgac cttctaacgt gtccggtcca gccactccgg
    46861 tgaaagctac actgtaatct gttcttgcta tctcacgtgc ttgaatggcc atctgctcca
    46921 cacattcttt gctgactgtt ccatattctg ctaatagctc cggatcaatc tccagaaaac
    46981 cagcttttgt ttggagcgag taggtaacga atccgcctgg aaatacttca gatacaccag
    47041 caatgtcgcc taatgcggct tggaaggcgc cggctgtcaa actttccgct gctgttacag
    47101 tctgcttgtt ttctttcagt agttccacta cgacttttgc tagcgaatta tcatcgccgt
    47161 atccataaaa atattcccct actcgttctt ggattttttc ttctaatgct aaaagagctt
    47221 gattccctgc ctgaacatca tttgttttca ctgtcagtcg taatgtcact tcatttggtt
    47281 tggcatatgg tgcgatcgtt ggattgatct gtgtctcaat cagatctttt agctcagtga
    47341 ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt agaaattagt ttttcttcag
    47401 aaggaaaacg ttcctctagt aaaggacgta cttgttccac aaacattggt ttcaactcat
    47461 taggcggacc gggaagaagg atatacgcat gtgtatctgt ttgataaaaa gtccctaaag
    47521 caagccctgt acgatttggt agaggaaccc cgccttcgat aatctgggac tgttgcagat
    47581 tgtttttggt catttttcga tgggttgttt caaaatatgc caataatttt ttgtatcctt
    47641 ccgtattttg tatgagagac ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat
    47701 cttctgtcgg tcctaatccg ccacataaga gtatgagttc acttcttgtt tcagctagag
    47761 caatcaactc ttctaaacgc tgctgattat ctcctacaac tgtttgataa taaacttcga
    47821 tacctagatc agctagctgt tctgacaaga aagtggcatt cgtattcacg acttgtccta
    47881 gtaaaagttc tgttccgaca gctattattt ctgctttcat tacttcccgc tcccttatat
    47941 agtatacgct gtatttttat atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa
    48001 atcttgtcac agctccttgt tccaaataaa cagtgttctc ctgccaaaac ctcggtcata
    48061 agtcacaata tacataaaac atgaaaagct gttttttgaa attatccctt gtgactcaga
    48121 gttagacggc aggctcacaa cctctccact aaacgatact tacattgatc ctttgaacac
    48181 accgctgttt ttgatgaagt aatccacacc tgaatagata gtaaagatca gacaagcata
    48241 aagcatgatt tgatctactg gaataccgat caggctgaat gggatattat tgataaacag
    48301 caagataata gcaaccattt gtgtagccgt cttgactttt cccggccaag ctgcagccat
    48361 gacttctcca tgttcgacaa gtagtaaacg caagcctgtg acggctaatt cacgacagac
    48421 aatgatcgct actacccaac caggggcctt tccttggtca accaacatga taaaagcagt
    48481 cataactagc attttatctg ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc
    48541 tcgcgcacgg gcaatttttc catctaacca gtctgtgata ctagctaaag caaaaataac
    48601 tgcacctact agctgtgtga cagccagtgt ggtgtctcct acgttaaatt gtccccaatc
    48661 taaaggtaaa cctacaacca aaatgaaaat cgggatcata caaattctaa gtacagttaa
    48721 tttatttggt aaattcacac tatcgactcc tttcttccct attctacgtg actatcagcg
    48781 aagtttccac cgatattatt tacttttcca aatgaattta ttcaaaggag tatttttatt
    48841 gaaattgatc tttttgaatt aacgaagaag gaccgcgaca cagatttgtc acgatccttc
    48901 ttttttatac ctacaagtaa acagatgcgt aaactaattt atacgatcat tctgcgtatt
    48961 gaagagtaag gttcacattt tttggactct gattgttttc ccctggatta actggtacgg
    49021 attctccatt cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg
    49081 ttgctgtttc cggtaacacg gtactctgtg tttcatttgc tgccagtgtc ccttgataaa
    49141 cataggcatt gtctactagc acacctaacc atacacgatt gacagcagta aattcaaggg
    49201 ttacaggctt ggtagcgtct gtgatattta ctgcgatggc actgccagaa tcttctcctg
    49261 tagctattgc cattttcttt tcttcacttg attttgttgt tgattctact gtagaagact
    49321 ctttggtctg cgtgctttgc tctgaggaag aggactcctc gactgatcct tctactttaa
    49381 gcgaagaagc tgtgtctccg attattggat cagcttgacg atcttgccaa gtcatatagc
    49441 ccacgacaac gacaatcgcc aaagcaacca atcctaataa aatgacagga agacttgtcc
    49501 aaaagcgact tggatgcgat tcttcctgat gcattgcttt tctagaagta cttactgttt
    49561 caggctgcgg acgttcaggg accgatcctg tcaatttgct tttcccatca aaaacatcga
    49621 ctaaatggtc tccatcttct cctacagctt cagcgtattg ccgaataaat gcccgcacgt
    49681 aaaatttacc aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa taacgttttt
    49741 gaatttttgt aatctgctgt aattcatcta aagatatttt tttattcagg cgtgcttgcc
    49801 gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc
    49861 tccgaagcat ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga caaaagtcat
    49921 gtcacagctc cttttttccg aataaacatt gttctcctgc caactcctcg gtcacaagtc
    49981 acaatatcca ataaatataa gaagctgttt tttgaacttg ttccttgtga ctcggagtta
    50041 aacggcaggc tcacgacctc tttcttaaac ggtgttcaat cagctgttcc tcggtattag
    50101 ctcaaaaacc ggcaaaatat aaggagatat ttttccgtat gtttctctaa tactcaggtc
    50161 aaaacgctga tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
    50221 taagtccaac caagttaaaa acattaagaa gctgttttta cttggctgtt cttattactt
    50281 gatgcagggc agctttttca caacctcttg gtacacggtg ttccagaagt agcttcttcg
    50341 gcaataagcc gaaatatcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    50401 cttgaagctg accacttctg tcacaacctc tttgattact tcgtttgatt ttattttaat
    50461 ataagttgat taaaagagag catgatttgt tttattttct cactaatttt tccaacacaa
    50521 ctaagaatac cataaaagct gattttggag gaggttccta ctacaagtct aaaacgaaat
    50581 ttaagacttt tcctcataaa atactggttt attttggtct catgtaaaac tcgctgaagg
    50641 cctcttcact tataaatgct tccccagctg ccagtacgtc tttcatttgg atcgaatcga
    50701 tgatttcagg aagatcaaat aaagttcgat cgccaaataa actttgcgta aattgattgg
    50761 caatatattc gatcgaattt aatgattgga aatattgtcc aagcattttc ttcttcagca
    50821 aatcgagatt tgtttctgac acttccggat catcagcgaa tccaagaatg atttctttga
    50881 ctttttctgc tgctttctct ggttcgtccg tatctccgct gaagtccgca aagtgaaatt
    50941 cacgatccaa gctgaattca aaaccaaagc tgtcatcgat aattccttga ttgtacatcg
    51001 ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa caacagattg attgctgttt
    51061 tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc taatccttta ataccaagta
    51121 caaatttatc tcgtgtaatg gctgcttcca atgcactttg tttgatgatt tcttttgtgt
    51181 tttcagggaa ataacgtaca atttcttgct ttggagggaa attttttgct tcttggtttt
    51241 cccgaataag tttcatcaat ttctcaggct ccatcttacc aactacaaat aaaaccatat
    51301 tgcttggctg ataaaacgtc cgatagcacg tatacaagtc ttgagctgtg attttgtcga
    51361 tactttcaac cgtgcccgca atatcgatat gcaatgggtg tgtaggatac aagttattta
    51421 gtatgccaaa aaacatccgc cagttagggt cgtcttcata catttggatt tcctgaccga
    51481 tgattccttt ttccttgttt acagtttctt ctgtgaagta aggtgcttgt acaaaatcga
    51541 tcagagtcgt cagattcttt tctacctgat ccgtcgtaga aaacagatag ctggttttcg
    51601 taaaacttgt gaaagcattg gcggaagctc cttgtttccc aaataattga aagacatcac
    51661 catcttcttt ttcaaacaac ttatgttcta aaaaatgggc aattccatct ggaactttca
    51721 ccttttcttt ttcaccatag ggaataaatt cgttatcgat cgaaccataa ttcgtactga
    51781 acaaaccata tgtcttgtga tagtcatttt taggcaacaa atacacggtc aaaccgtttg
    51841 gcagtacttc atgataaagt gtttcattga tttgctcata ttctgtttta ttcatttgca
    51901 tttcctcctg ctaaaaagaa gattgcctgc aattcgattt gctcagctac ttgttgtatc
    51961 tctttgatcg ttacgctctc catacgtttc aaccattctt catcagataa ttttgtttcc
    52021 ggcagccagc tgtccaaaaa agaagcttcg attgccgctt gcggagagtc tagagaaagc
    52081 agatattggt ttctcagcat tgcttttgtc tgagacagtt ccaactctgt tatttcacca
    52141 tttcgcaaac tttctaattg ctcgtgaatc agatgaagta cttgatttcg gttcttttca
    52201 tcgataccgg tttggacact catgaatcct cggaacgtat ccacgctact agaggcataa
    52261 taagctagac tttctttctc tctgacattc atgaaaagtt tagagtgagg aaaaccgcca
    52321 aataaaccgt taaagaccat tagcgcaaaa cgttctggtt catcgtaata cacatttgtc
    52381 tgataagcta ggttcagctt tgcttgaacg attggctcac gtacttgtcg ttccttcacg
    52441 atattcactt gaggttgggt ataaaacaga tctggataaa ctgtttcccg cgactcaaat
    52501 ggtaattgcc caactgcttc agctgctttg tccggatcaa catcgccgac aacaaaaatg
    52561 tcgatttgat catttgccat catttcttga tagtaggacg ctagcgaact agcagtgatc
    52621 ttgtccaaat cttcgactac gcccaaccct gggatctttt gttccgggct gttttcaaaa
    52681 taaagttctt gcaaggcata ggaagcaaaa gtttgtttat cttccttcaa actttccaaa
    52741 tatagacgca gattgttctt ttccaagtca aacgtcaact gatcaaactg atgattttta
    52801 atatttggat aaaataaaac ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga
    52861 tcattgacat attcaccatt cacaaaactg atccctgcgt ttagccaatg taaatttcct
    52921 ttttttctaa ctgataatcc aaagcttgcg ccataaagct ctgcaagttg cgcacttaac
    52981 tttgtctgat ccgaataatt caaactattt gtttccaaca tgctgctgag aagtgaacgt
    53041 ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga taaaaatccg gatcgttttg
    53101 tattgttttg ttggtaagac atgaagattc acgccttctg ccaattgata caccatattt
    53161 atatgctcct tttcacagtt gattgcccgc ttactctttt aatgggtcaa cctgcctttt
    53221 ttacttattc ctctgcttga aatttataag ttcaggcaat tcgccgtttt ttctaaatga
    53281 tgttacactt tatagtatag catagatgtt ttttatcaaa agaaacagac ggctaaaagc
    53341 gtatattgtt tttttatttt ttatttttcg atatactaaa gacaaataaa agaagggagg
    53401 gtcattcatg atcaaagaat tcaaagagtt catcatgcgt ggagatgtct tggatcttgc
    53461 tgtcggcgtt gttattggta gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt
    53521 gatcacacca ctaataggtt ggtttgtcgc aatgatcaca ggtacttctg atttagaagg
    53581 agcactttca attcttgact ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt
    53641 tattagtgct atcattacat ttctgatcac tggttttgtc ttattcttag ttgtcaaagc
    53701 ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa gtggaagtgg aagaaactcc
    53761 tactgcagaa gattatctta gtgacatacg cgatcttctt gccaaacaag tggcagaaga
    53821 caatacacat ccagtagagc aaacaaatct ttccgatact tctaaataat aaagaatcta
    53881 aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac
    53941 ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc
    54001 cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg
    54061 tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa
    54121 accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc
    54181 ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca
    54241 agcttctcca tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac
    54301 tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt
    54361 tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc
    54421 acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca
    54481 gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt
    54541 tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt
    54601 aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg
    54661 ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc
    54721 acctctacta tcaactatac cacatatttt taaaattttc aagtttacca taaaaaaaaa
    54781 agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta ttcctcatta
    54841 taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc
    54901 ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa
    54961 tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt
    55021 aataaaagat gctgaattgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt
    55081 ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc
    55141 gcgttctgct tcctcaacag agaatagatt tgattgctta taacgctttt ataataactt
    55201 ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt
    55261 gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt
    55321 agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt
    55381 gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga
    55441 atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat
    55501 taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg gagaattacg
    55561 tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt
    55621 tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca
    55681 ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc
    55741 agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa
    55801 ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat
    55861 gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc
    55921 atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt
    55981 gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa
    56041 agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt
    56101 catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc
    56161 ttcattacca atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc
    56221 ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac
    56281 caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac
    56341 gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc
    56401 gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa
    56461 aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata
    56521 ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc
    56581 taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag
    56641 ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg
    56701 ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc
    56761 attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg
    56821 ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt
    56881 tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg
    56941 tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat
    57001 cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt
    57061 cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg
    57121 cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa
    57181 tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac
    57241 cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc
    57301 ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc
    57361 acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa
    57421 ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc
    57481 ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc
    57541 aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat
    57601 gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc
    57661 tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt
    57721 ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt
    57781 cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat
    57841 gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt
    57901 gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt
    57961 ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa
    58021 atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg
    58081 tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc
    58141 ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat
    58201 ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc
    58261 tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg
    58321 catatgatcc atgactcttg gatgcagtgt agcaggttcg acattcaacg ctttcgtatc
    58381 ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt
    58441 tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata
    58501 gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc
    58561 gatatggata taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg
    58621 tatagggatg aattcttctt tttttctggt caatgtattg tagattttta tcattctgat
    58681 ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg
    58741 tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc
    58801 gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg
    58861 tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca
    58921 tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg
    58981 ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga
    59041 gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc
    59101 caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc
    59161 ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc
    59221 tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa
    59281 taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat
    59341 tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt
    59401 ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat
    59461 tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt
    59521 catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat
    59581 tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc
    59641 atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg
    59701 acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca
    59761 gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca
    59821 ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac
    59881 aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt
    59941 ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg
    60001 atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt
    60061 aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc
    60121 acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat
    60181 tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct
    60241 gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct
    60301 tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga
    60361 gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg
    60421 cgtttttgga tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc
    60481 accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct
    60541 agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg
    60601 taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct
    60661 gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag
    60721 atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca
    60781 tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata
    60841 ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca
    60901 aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt
    60961 ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga
    61021 ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta aagccactca
    61081 gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca
    61141 gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt
    61201 gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg
    61261 aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac
    61321 gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt
    61381 tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa
    61441 cgcattgata ttaagaacag gtacattttg gaattcagag actttattca agttatagtc
    61501 attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc
    61561 actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa
    61621 ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc
    61681 cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa
    61741 tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt
    61801 atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc
    61861 tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg
    61921 tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac
    61981 actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt
    62041 caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag
    62101 tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca aagataataa
    62161 aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga
    62221 ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac
    62281 aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc
    62341 atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat
    62401 ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt
    62461 gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc
    62521 tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata
    62581 gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc
    62641 acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag
    62701 cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt
    62761 tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc
    62821 cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg
    62881 tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt
    62941 cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc
    63001 ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc
    63061 ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag cggttttgag
    63121 tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg
    63181 ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc
    63241 acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc
    63301 gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac
    63361 ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat
    63421 acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg
    63481 gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat
    63541 ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc
    63601 ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa
    63661 cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat
    63721 tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg
    63781 ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct
    63841 gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca
    63901 tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg
    63961 taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga
    64021 ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca
    64081 gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca
    64141 tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc
    64201 tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg
    64261 atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct
    64321 ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa
    64381 ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac
    64441 tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata
    64501 gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat
    64561 gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc
    64621 aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag
    64681 gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca
    64741 gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg
    64801 atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg
    64861 acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag
    64921 cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa
    64981 ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt
    65041 gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg
    65101 actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg
    65161 gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa
    65221 gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg
    65281 gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg
    65341 aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac
    65401 ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga
    65461 atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata
    65521 tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta
    65581 cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga
    65641 cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg atcggcatca
    65701 tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga
    65761 atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat
    65821 tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg
    65881 atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga
    65941 ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca
    66001 ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg
    66061 cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa
    66121 cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg
    66181 tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg
    66241 ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag
    66301 tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag
    66361 aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg
    66421 cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg
    66481 cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat
    66541 taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt
    66601 gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg
    66661 tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg
    66721 cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga
    66781 gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac
    66841 gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt
    66901 ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat
    66961 cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg
    67021 tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg
    67081 acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg
    67141 gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat
    67201 aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt
    67261 cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt
    67321 ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca
    67381 accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat
    67441 attcttccag catctattat ccccccattc tttttcttaa atgaatttta gcttacctac
    67501 tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca
    67561 catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat
    67621 attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga
    67681 cagtctcaca acctcttggt aaacggcgtt caatcagctg accctcggta ttagctcaaa
    67741 aaacggtaaa atatgagaag ctatttttcc gttttgtttc tctaatactt aggataaaac
    67801 gctgatttta caacctctgt taaacggtgt tcaatcagct gtccttcggt attagctcaa
    67861 aaaacggcaa aatatgagga actatttttc cgtttgtttc tctaatactc aggacaaaac
    67921 gctgatttta caacctcctg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    67981 caaccaaacg aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag
    68041 gacagctttt tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg
    68101 gtgataaatg atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata
    68161 gctttcacca atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa
    68221 aaagcggaca aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat
    68281 agcaatcgtc gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct
    68341 gatcggtaca aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa
    68401 taagacaaat gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc
    68461 aacgaaaatc gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc
    68521 taattcagaa tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa
    68581 attatataac aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac
    68641 accttcccaa aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag
    68701 ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc
    68761 actgcccaag accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc
    68821 atacatcatg attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc
    68881 agtagagaca aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc
    68941 gatattccca gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat
    69001 caatgttttt ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc
    69061 agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg
    69121 tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca
    69181 ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg
    69241 tatatctatt tgattctcca atatttccat ccctttctct ctattagcat catacatgat
    69301 ttattttaca tgtcaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag
    69361 aaaataagac gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc
    69421 ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag
    69481 cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg
    69541 ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg
    69601 ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc
    69661 tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca
    69721 ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct
    69781 cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt
    69841 gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt
    69901 aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa
    69961 gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg
    70021 atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt
    70081 tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa
    70141 agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca
    70201 ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa
    70261 gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc
    70321 ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg
    70381 aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat
    70441 aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa
    70501 cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc
    70561 tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt
    70621 tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc
    70681 atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat
    70741 cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt
    70801 aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt
    70861 cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga
    70921 aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc
    70981 cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt
    71041 gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt
    71101 tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa
    71161 ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg
    71221 aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct
    71281 ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga
    71341 agacaaccga tccagtctga tttgttgctt tttcttttta ctggacactt tttccaactt
    71401 ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat
    71461 cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact
    71521 ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag
    71581 ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag
    71641 ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag
    71701 catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc
    71761 gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa
    71821 atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc
    71881 tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga
    71941 tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga
    72001 atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc
    72061 taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa
    72121 aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga
    72181 aataactatg actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga
    72241 aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta
    72301 gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt
    72361 ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa
    72421 accttttatt cgaacatagt gatcttttct ggtgctaaac gaacagactc gtaaccttct
    72481 tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt atccaaacca
    72541 aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc atagccatga
    72601 tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg
    72661 ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct
    72721 ctgacttctt ttggcatctt ccctgattcg taagcttggt tgtagatttc ttcgccatat
    72781 tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc
    72841 gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca
    72901 aagcgccatg gtttcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc
    72961 agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca
    73021 gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt
    73081 tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc
    73141 tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg
    73201 cttgattttc ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg
    73261 atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc
    73321 tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt
    73381 cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt caaagatctg
    73441 gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga
    73501 cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata
    73561 aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag
    73621 tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc
    73681 taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg cagtagataa
    73741 ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa
    73801 attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt
    73861 ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa
    73921 acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac
    73981 tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag
    74041 ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt
    74101 aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca
    74161 accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact
    74221 gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc
    74281 ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca atgtatataa
    74341 atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt
    74401 atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg
    74461 cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct
    74521 tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt
    74581 tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt
    74641 aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc cattatagct
    74701 tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc
    74761 actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa
    74821 actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg
    74881 agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg
    74941 tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt
    75001 ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg
    75061 ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac tttctgtatc
    75121 gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc
    75181 ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa
    75241 taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag
    75301 tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt
    75361 aataaatttg ctttttctgc ataaaaagac gtaaatgaag aatattcttc tccattcacg
    75421 ccttaaattg atggtcgttt gaataactat ttaacaagta attctctctg gatattatcc
    75481 gttatggatt atacttcctg tgtcgtcgga cggataatca tgttgctgac agacatccgg
    75541 tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcagatgt taatccccat
    75601 tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat
    75661 tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct
    75721 tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa
    75781 tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt
    75841 ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca
    75901 agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct
    75961 gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct
    76021 tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa
    76081 gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct
    76141 ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca
    76201 atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct
    76261 tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg
    76321 attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca
    76381 ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct
    76441 ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct tcgttctttt
    76501 ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct
    76561 tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc
    76621 cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca
    76681 tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac
    76741 ccagtattca gcgctgctaa aggctcttcg ttttccgctt ggatctgatg gatcacttca
    76801 aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc
    76861 attttgctcc ctccttttat ccttacttta taacggacgg cttatgatgt ctaatactta
    76921 taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg
    76981 agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat
    77041 atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa
    77101 gatttagaag aagaacttgg tgtgacactt ttcactcgag gctcccgaga gatacagttg
    77161 acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa
    77221 acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc
    77281 gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct
    77341 gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc
    77401 ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt
    77461 cgactgccag cccaagatcg ttggggactt ttgatacatc ggaatcatcc tttagcaaag
    77521 caaacagtca tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg
    77581 tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc
    77641 ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca
    77701 ttagctctag atgggatcat caatacttat gggacggaaa aagtattcat cccactcgat
    77761 ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct
    77821 gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc
    77881 gctttcagct ttttaacaac gaataagtta agcaaaagat ccaatgatta aataacataa
    77941 gtttcttaat ttgttccttc cttttatttc tgataattcc agtaaattac tataattata
    78001 aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa caataattcg
    78061 tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg tttccctagg
    78121 aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc
    78181 aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat
    78241 tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga tttgacagaa
    78301 aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg gaaccagaag
    78361 gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta tttgcagcag
    78421 aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa catgtattga
    78481 tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt atcgtcatcc
    78541 gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt attgctcaat
    78601 gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac gaagcaggat
    78661 atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat cgcagtatcc
    78721 gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg gaaacagata
    78781 tccattcaga accattttca ctgaattatt caagactggt gaatcattta cgactgcttc
    78841 tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta gaaatggtca
    78901 aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag
    78961 aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat atcgagcgtt
    79021 taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa aattgaaagc
    79081 atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac ctgctgctgc
    79141 tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg tcgctcattt
    79201 tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg ccgttgggtt
    79261 agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt
    79321 tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga atatcaaacc
    79381 agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta ttattgccgg
    79441 attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga tggctttatc
    79501 tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt tgctgatcag
    79561 tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt ttgctaaatt
    79621 cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc gtttactgat
    79681 ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag caggaatcaa
    79741 tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc
    79801 tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta tttacagaaa
    79861 tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ttagcagcag cttttgcttc
    79921 attctttact ggtgtaacag aaccacttga attctcattt atgttcgttg cttggccatt
    79981 atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat tcttccattg
    80041 gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc
    80101 gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag tgatttacta
    80161 ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc
    80221 agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg cggcacaagc
    80281 aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg ataactgtac
    80341 tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata aaatcaaagc
    80401 cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac
    80461 agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg caacacgacc
    80521 tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtggaacga tcgaacaaga
    80581 tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag atgatgtatt
    80641 cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag aaatcttctc
    80701 tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg gaatccaaac
    80761 agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact taaaaggcga
    80821 accattcact ttatatgtaa aagaaggtca aaaagtcgca agaggtcaat tgattgcttt
    80881 agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg ttgtattcac
    80941 aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga
    81001 taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac ttttttatcg
    81061 ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg aataaaaacg
    81121 gcgttctcct gccaactccg cggtaataag tccagccaaa cgtaacatat gaagagctat
    81181 tttttatttg actcttctta ttacttgatg cagaacagct tttttacaac ctctgataaa
    81241 cggtgttcaa aaaccagttt atcggtatga agtcaaattt atgcaaaaat gtggaaagcc
    81301 attttcacaa aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata
    81361 cattattcct gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc
    81421 aatacttttt ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg
    81481 atcgctaaaa acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca
    81541 aacgctgcat cacttgcttc aataatttct aactttttac ctgtcgcacg ctctaatgcc
    81601 ttggctaatt cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca
    81661 ctttctacta gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt
    81721 tttccatttc ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt
    81781 aattcattct ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta
    81841 atcatttttt cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct
    81901 gcaaaacttg tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc
    81961 ttatgttcgg cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct
    82021 ttcaacgctt ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca
    82081 gcttctttca gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt
    82141 ggaaccatct tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt
    82201 actaaataat gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta
    82261 catttataaa ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat
    82321 gaaaggatga caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc
    82381 ctatattgaa atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat
    82441 cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat
    82501 ctttacaaaa accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt
    82561 actagatgtc tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac
    82621 aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg
    82681 gctgcagaaa aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa
    82741 aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt
    82801 ataattctct ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta
    82861 ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag
    82921 cgctgaagtc atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc
    82981 tgaaaaatgc aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa
    83041 gcgctacttt cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct
    83101 cccatatatt tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag
    83161 gcgtaatctt ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt
    83221 aattcttcag gcgattctgc taaagcgatt acaacaagat acgttccttt tgggaatggg
    83281 atgattcggg tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt
    83341 ttcccgttat agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat
    83401 gcatctattg ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa
    83461 ccgacaaaat ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct
    83521 ggtttttctt cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca
    83581 tcttacataa aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta
    83641 ttttttaact atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa
    83701 tcctctcccc attttgtaaa agagatctcg ggagcttcga ctaactcata atctgaagaa
    83761 ggaagccatt ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct
    83821 ttattaggaa aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac
    83881 ggattttctt ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag
    83941 cgggctccat caaatccata agaaacgttg attacttgat ttggatataa atctcctaac
    84001 ttatgcatct cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga
    84061 ttgactcctt caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct
    84121 ttcttttcga tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag
    84181 cgtggaaaag atttttgtat gccgtatctg ctgatttctg aaggcagata tcctgaccaa
    84241 tcacgaaaag ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct
    84301 gtcactcttg atccatctag gagatcgcga ttagccaaag ccaatctacg gtttttgata
    84361 tagtccatta gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag
    84421 attcctgtat tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg
    84481 taatcgatca attggttcag atctttaagc atatttatct tcctccttgc ttgccttttt
    84541 atcatattat caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta
    84601 gaaaatgttg tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt
    84661 attatctgcc tatctatgtt ttacctttag aaaaataaat tttggggaag acacattcta
    84721 agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg
    84781 tacaaagcaa attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga
    84841 tacatcatac ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt
    84901 tttgtcagat aaataaaa
//
LOCUS       DARBFD010000003        84112 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000003 DARBFD010000000
VERSION     DARBFD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.003"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            159..851
                     /locus_tag="SCK08_000157"
     CDS             159..851
                     /locus_tag="SCK08_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1200079.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1059..1349
                     /locus_tag="SCK08_000158"
     CDS             1059..1349
                     /locus_tag="SCK08_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200080.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            2063..3433
                     /locus_tag="SCK08_000159"
     CDS             2063..3433
                     /locus_tag="SCK08_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200081.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(3673..3756)
                     /locus_tag="SCK08_000160"
     tRNA            complement(3673..3756)
                     /locus_tag="SCK08_000160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3720..3722),aa:Leu,seq:gag)
     gene            3811..3990
                     /locus_tag="SCK08_000161"
     CDS             3811..3990
                     /locus_tag="SCK08_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200082.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            4139..5356
                     /locus_tag="SCK08_000162"
     CDS             4139..5356
                     /locus_tag="SCK08_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1200083.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            5540..6376
                     /locus_tag="SCK08_000163"
     CDS             5540..6376
                     /locus_tag="SCK08_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1200084.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            6452..7600
                     /gene="nagA"
                     /locus_tag="SCK08_000164"
     CDS             6452..7600
                     /gene="nagA"
                     /locus_tag="SCK08_000164"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEG1200085.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            7820..8878
                     /locus_tag="SCK08_000165"
     CDS             7820..8878
                     /locus_tag="SCK08_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEG1200086.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            8882..9916
                     /locus_tag="SCK08_000166"
     CDS             8882..9916
                     /locus_tag="SCK08_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEG1200087.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(10018..10734)
                     /locus_tag="SCK08_000167"
     CDS             complement(10018..10734)
                     /locus_tag="SCK08_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1200088.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(10703..11488)
                     /locus_tag="SCK08_000168"
     CDS             complement(10703..11488)
                     /locus_tag="SCK08_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEG1200089.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(11479..12228)
                     /locus_tag="SCK08_000169"
     CDS             complement(11479..12228)
                     /locus_tag="SCK08_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1200090.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            12526..13050
                     /locus_tag="SCK08_000170"
     CDS             12526..13050
                     /locus_tag="SCK08_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEG1200091.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            13066..14565
                     /locus_tag="SCK08_000171"
     CDS             13066..14565
                     /locus_tag="SCK08_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEG1200092.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(14653..15255)
                     /locus_tag="SCK08_000172"
     CDS             complement(14653..15255)
                     /locus_tag="SCK08_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1200093.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            15359..16315
                     /locus_tag="SCK08_000173"
     CDS             15359..16315
                     /locus_tag="SCK08_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEG1200094.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(16620..17576)
                     /locus_tag="SCK08_000174"
     CDS             complement(16620..17576)
                     /locus_tag="SCK08_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1200095.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            17750..18310
                     /locus_tag="SCK08_000175"
     CDS             17750..18310
                     /locus_tag="SCK08_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200096.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            18303..19127
                     /locus_tag="SCK08_000176"
     CDS             18303..19127
                     /locus_tag="SCK08_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEG1200097.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            19137..19754
                     /locus_tag="SCK08_000177"
     CDS             19137..19754
                     /locus_tag="SCK08_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1200098.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            19772..20455
                     /locus_tag="SCK08_000178"
     CDS             19772..20455
                     /locus_tag="SCK08_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEG1200099.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            21138..22373
                     /locus_tag="SCK08_000179"
                     /pseudo
     CDS             21138..22373
                     /locus_tag="SCK08_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            22381..23268
                     /locus_tag="SCK08_000180"
     CDS             22381..23268
                     /locus_tag="SCK08_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1200100.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            23285..24121
                     /locus_tag="SCK08_000181"
     CDS             23285..24121
                     /locus_tag="SCK08_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1200101.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            24124..24747
                     /locus_tag="SCK08_000182"
     CDS             24124..24747
                     /locus_tag="SCK08_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200102.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            24744..26036
                     /locus_tag="SCK08_000183"
     CDS             24744..26036
                     /locus_tag="SCK08_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1200103.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      26395..26492
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            26608..27189
                     /locus_tag="SCK08_000184"
     CDS             26608..27189
                     /locus_tag="SCK08_000184"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEG1200104.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            27192..28499
                     /locus_tag="SCK08_000185"
     CDS             27192..28499
                     /locus_tag="SCK08_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEG1200105.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            28792..29280
                     /gene="purE"
                     /locus_tag="SCK08_000186"
     CDS             28792..29280
                     /gene="purE"
                     /locus_tag="SCK08_000186"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEG1200106.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            29273..30397
                     /gene="purK"
                     /locus_tag="SCK08_000187"
     CDS             29273..30397
                     /gene="purK"
                     /locus_tag="SCK08_000187"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEG1200107.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            30410..31705
                     /gene="purB"
                     /locus_tag="SCK08_000188"
     CDS             30410..31705
                     /gene="purB"
                     /locus_tag="SCK08_000188"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEG1200108.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(31885..32262)
                     /locus_tag="SCK08_000189"
     CDS             complement(31885..32262)
                     /locus_tag="SCK08_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200109.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            32518..33246
                     /locus_tag="SCK08_000190"
     CDS             32518..33246
                     /locus_tag="SCK08_000190"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEG1200110.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            33246..33500
                     /gene="purS"
                     /locus_tag="SCK08_000191"
     CDS             33246..33500
                     /gene="purS"
                     /locus_tag="SCK08_000191"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEG1200111.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            33502..34173
                     /gene="purQ"
                     /locus_tag="SCK08_000192"
     CDS             33502..34173
                     /gene="purQ"
                     /locus_tag="SCK08_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEG1200112.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            34174..36396
                     /gene="purL"
                     /locus_tag="SCK08_000193"
     CDS             34174..36396
                     /gene="purL"
                     /locus_tag="SCK08_000193"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEG1200113.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            36381..37820
                     /locus_tag="SCK08_000194"
     CDS             36381..37820
                     /locus_tag="SCK08_000194"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEG1200114.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            37843..38895
                     /locus_tag="SCK08_000195"
     CDS             37843..38895
                     /locus_tag="SCK08_000195"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEG1200115.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            38892..39470
                     /gene="purN"
                     /locus_tag="SCK08_000196"
     CDS             38892..39470
                     /gene="purN"
                     /locus_tag="SCK08_000196"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEG1200116.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            39467..41002
                     /gene="purH"
                     /locus_tag="SCK08_000197"
     CDS             39467..41002
                     /gene="purH"
                     /locus_tag="SCK08_000197"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEG1200117.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            41018..42292
                     /gene="purD"
                     /locus_tag="SCK08_000198"
     CDS             41018..42292
                     /gene="purD"
                     /locus_tag="SCK08_000198"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEG1200118.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            42414..43328
                     /locus_tag="SCK08_000199"
     CDS             42414..43328
                     /locus_tag="SCK08_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1200119.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(43461..45026)
                     /locus_tag="SCK08_000200"
     CDS             complement(43461..45026)
                     /locus_tag="SCK08_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1200120.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(45019..46470)
                     /locus_tag="SCK08_000201"
     CDS             complement(45019..46470)
                     /locus_tag="SCK08_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1200121.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(46484..47500)
                     /locus_tag="SCK08_000202"
     CDS             complement(46484..47500)
                     /locus_tag="SCK08_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1200122.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            47720..48808
                     /locus_tag="SCK08_000203"
     CDS             47720..48808
                     /locus_tag="SCK08_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEG1200123.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            48862..50388
                     /locus_tag="SCK08_000204"
     CDS             48862..50388
                     /locus_tag="SCK08_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEG1200124.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            50390..51595
                     /locus_tag="SCK08_000205"
     CDS             50390..51595
                     /locus_tag="SCK08_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1200125.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            51852..52829
                     /gene="guaC"
                     /locus_tag="SCK08_000206"
     CDS             51852..52829
                     /gene="guaC"
                     /locus_tag="SCK08_000206"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEG1200126.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            53093..54517
                     /locus_tag="SCK08_000207"
     CDS             53093..54517
                     /locus_tag="SCK08_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1200127.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            54978..56087
                     /gene="pdhA"
                     /locus_tag="SCK08_000208"
     CDS             54978..56087
                     /gene="pdhA"
                     /locus_tag="SCK08_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEG1200128.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            56090..57067
                     /locus_tag="SCK08_000209"
     CDS             56090..57067
                     /locus_tag="SCK08_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEG1200129.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            57092..58735
                     /locus_tag="SCK08_000210"
     CDS             57092..58735
                     /locus_tag="SCK08_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEG1200130.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            58743..60149
                     /gene="lpdA"
                     /locus_tag="SCK08_000211"
     CDS             58743..60149
                     /gene="lpdA"
                     /locus_tag="SCK08_000211"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEG1200131.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            60311..60586
                     /locus_tag="SCK08_000212"
     CDS             60311..60586
                     /locus_tag="SCK08_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEG1200132.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            60592..61359
                     /locus_tag="SCK08_000213"
     CDS             60592..61359
                     /locus_tag="SCK08_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEG1200133.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            61527..63356
                     /gene="typA"
                     /locus_tag="SCK08_000214"
     CDS             61527..63356
                     /gene="typA"
                     /locus_tag="SCK08_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEG1200134.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            63617..65389
                     /gene="recQ"
                     /locus_tag="SCK08_000215"
     CDS             63617..65389
                     /gene="recQ"
                     /locus_tag="SCK08_000215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEG1200135.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            65503..65805
                     /locus_tag="SCK08_000216"
     CDS             65503..65805
                     /locus_tag="SCK08_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEG1200136.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            65808..66971
                     /locus_tag="SCK08_000217"
     CDS             65808..66971
                     /locus_tag="SCK08_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1200137.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            66997..70425
                     /locus_tag="SCK08_000218"
     CDS             66997..70425
                     /locus_tag="SCK08_000218"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEG1200138.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            70446..71570
                     /locus_tag="SCK08_000219"
     CDS             70446..71570
                     /locus_tag="SCK08_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200139.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            71560..71997
                     /locus_tag="SCK08_000220"
     CDS             71560..71997
                     /locus_tag="SCK08_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1200140.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            71981..72319
                     /locus_tag="SCK08_000221"
     CDS             71981..72319
                     /locus_tag="SCK08_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEG1200141.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            72438..72998
                     /gene="rsmD"
                     /locus_tag="SCK08_000222"
     CDS             72438..72998
                     /gene="rsmD"
                     /locus_tag="SCK08_000222"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEG1200142.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            72995..73486
                     /gene="coaD"
                     /locus_tag="SCK08_000223"
     CDS             72995..73486
                     /gene="coaD"
                     /locus_tag="SCK08_000223"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEG1200143.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            73476..74519
                     /locus_tag="SCK08_000224"
     CDS             73476..74519
                     /locus_tag="SCK08_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEG1200144.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            74678..75406
                     /locus_tag="SCK08_000225"
     CDS             74678..75406
                     /locus_tag="SCK08_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEG1200145.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            75522..75767
                     /locus_tag="SCK08_000226"
     CDS             75522..75767
                     /locus_tag="SCK08_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1200146.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            75764..75982
                     /locus_tag="SCK08_000227"
     CDS             75764..75982
                     /locus_tag="SCK08_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEG1200147.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            76118..76657
                     /locus_tag="SCK08_000228"
     CDS             76118..76657
                     /locus_tag="SCK08_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEG1200148.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            76650..78923
                     /locus_tag="SCK08_000229"
     CDS             76650..78923
                     /locus_tag="SCK08_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEG1200149.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            78961..80007
                     /gene="holA"
                     /locus_tag="SCK08_000230"
     CDS             78961..80007
                     /gene="holA"
                     /locus_tag="SCK08_000230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEG1200150.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(80096..80350)
                     /locus_tag="SCK08_000231"
     CDS             complement(80096..80350)
                     /locus_tag="SCK08_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEG1200151.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(80750..81019)
                     /locus_tag="SCK08_000232"
     CDS             complement(80750..81019)
                     /locus_tag="SCK08_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200152.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(81489..81944)
                     /locus_tag="SCK08_000233"
     CDS             complement(81489..81944)
                     /locus_tag="SCK08_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEG1200153.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(82082..84010)
                     /locus_tag="SCK08_000234"
     CDS             complement(82082..84010)
                     /locus_tag="SCK08_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1200154.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        27410 a        14377 c        18118 g        24207 t
ORIGIN      
        1 tataaaaaag attttttaaa tagataaaaa aggaggatta aatgagtatt tgatagtatt
       61 tatattaaaa ttacacatgt aatttaaaaa cgcttgacat tgtgtttgag atcacttact
      121 tttagcatgt aaaagtattg gattaaggag ctatgcaaat gaaaaagaaa tttattactg
      181 gtgggattgt cgttgcattt ctatgtctaa ttggctacac aggcacgcgg tacgcaaatg
      241 aaattatttt gtggggtgga gagacagata ttgatacaat caatgagaat ctgacacagc
      301 ttgatcatac attgacagat aaagagcaag cgcttacaga tcttaccgct cgactagcac
      361 aaaaagaaca gacattgcaa gattcaaagg cagatgtcca agaataccaa acaaaagtaa
      421 atgaattaga aaaccaaaaa aatcaatcgg cagcagaaaa acaaaactta gaaagtcaat
      481 tagtcagcaa ggatgcacaa ctacaaagca aacaaaatga aatcaatgtc aaactagatg
      541 agatcaacca aaaaaatgat gaaatcaatc atctgaatgc agattggaca gcaagactac
      601 aagaagcaca agcaaaactt tcagaattac aaaatactgt aaatggtttg aatcaacaaa
      661 tcgcaacact gacacaagaa aaaacaaatg tagaaactca gttaacagat acacaaaaga
      721 aattagacga agcaactgct gaaaactcaa gcttgaaaca atatattcaa aagttagaaa
      781 aagcaaaaga tgaagtggaa gatacagctg ataaatctca gcaaattgtt gaagaacatt
      841 tgcgtaaata atctttctta acaagtaaac ctaaattagt aaagaagcaa cagtaatcta
      901 tactgctgcc tctttttttt agtacatgtc cttcatcttc gcaggaagtg ccaatatgat
      961 tttaagaaag gtaatagaca aaaagtcaaa aagataaaac agagtttctt ctttacaaag
     1021 ggaatgtatg ttcgtataat ggtattgagg tgaaggttat ggagaaaaaa tatggaacag
     1081 tagataagat ccgtactttg caagttagtg ccagaccgtt gatccgtttc agtttaaatg
     1141 gggtcaattg tttgattgct tcccatagtt tgaacttcct agctgaagta gacgaaggga
     1201 tgaagttagt cgttacaggt tattataata atagaaagca atttgttgtg agatcctatc
     1261 atgtgttagg gaaacccaaa attgtggtag aatatgagaa aagtctatac ccaagaaaaa
     1321 aggtccgaca agatgaaaga ataaactaga aaacaagcgg ctgagacaaa aatgtcctag
     1381 ccgcttgttt tggaaagatg gatgcagacg atgggatttg aacccacacg agcatacgct
     1441 cacaagaacc tcaatctata tataactaca tgtatttaag tatttgtgat gaatcactaa
     1501 actttccaaa ctcatttgag ggatattgct taaagattga acagatcaat tcgaagccct
     1561 attagtattc gctcaatggg gggatcagag gccataaaaa gagactagtc attactagtc
     1621 tcaggtagtg gaattgtagg attttaaacg ttcctggaga gtaccggtat gtaactcgaa
     1681 gaggtgatta tcatagtcgt aaaagtaaat tgaagaagcc tcgcctttca cacgagaacg
     1741 tcctggttcg acagttaaac ctaacatttg gatttttgag acaaacgaat ctatatcttg
     1801 ttcgtcaatt ttaaaagcaa tatgattgta tgtctttggc agtttattcg acgaattctg
     1861 cattactgca atccataggt ccttaactaa gaaaaatttt tctggatata atgaaaattt
     1921 ttttgttgac tatcgtagat ttcttttgca tcaaataagg tatttaacaa ttaagtattt
     1981 aaaagataac ttttaagtta tctttaagta atgcttaaag ctatgttata ctctattagg
     2041 aaatataaga aggagtataa aaatgaacaa acataataga aaaataacga ataacaaaga
     2101 actgatcttg ttactaatag ggatttgggg tatcttttta ctctttaata tgtatatgcc
     2161 gactatgaga gcggatgacg tagtttatgc aagtagatta gatgaacttg gctatttggg
     2221 tgcttcgata gaacattata agacatggag ttcaagaatt attatagaac tattcttaat
     2281 gttcttttct aaacacttta tgctatggaa attattaaat tctacgatta tgttaggaat
     2341 tgtagtatta ctttgcaagt atgtttttga gaaaataaat gttaaaaatt tattattggt
     2401 ttgctctata tactgtttaa taccattgac agtaatgggg gaaaccggtt ggagagcaac
     2461 caccttgaac tatcagtggc cagtagcatt tagtttacta actttttatc cgttttttca
     2521 actcttaaga ggagaagaaa taaatagaaa aatatattgg gtaagtattc ctttattgat
     2581 atttttaact aatcaagaac aagtcaacgc ctgttttttt gttctaacaa gtatagttag
     2641 tttatatttg attgtcaatg gacgatataa ttataagtta tcagtatttt caataataag
     2701 cttagcagaa ttaattttct ctttgacaac tccagggaat gcattacgtg ctgcacatga
     2761 aattaataaa tggtttcctg aatataaaaa tttcaacttt cttaataagt tagatttagg
     2821 tatttcatca tttggaaagc cgtttttttt agacatgaat atcttatttt tattgctatt
     2881 tttcttaatt ttccttctaa catataggaa atgtcaaaac tattatgtgc gtatattgac
     2941 tgcattaccg tttttcttaa acctgattat ttattttggt aatacaatgg gtcaaagctt
     3001 tacttacgtc aatggaaata aaagggcaat gatttggagt agtagtaacc tgaataatct
     3061 ctttacagag ttaggaacga aactgtcact tttttatcca ggtacttgga tagctacttt
     3121 agtagtcttg gcattattac tatgtttaat tgttggaatt tatttaagtt ttgacaataa
     3181 gaaaacttct atttttttag ttattttgat gataatggga ttttgttcaa ggttaattat
     3241 gggattttct cccacagtat gggcttccgg aatgcggacg tactatatac tttatgttgt
     3301 tatcgccatc ttagtactaa tggcagttaa agagctaatg aaaagcatga gtgtacaaaa
     3361 aaatgaattt atgcaatttg gattaaccgt gcttggtatt tgtaccttta taataacggt
     3421 gataaacaga taatatacaa aaaatccctc gctttagtat taggtgaggg attttactcg
     3481 tttttcgatt aattgtttta acacttgcaa gtttctacga gtagcttgtt tccgaccttc
     3541 atatactcac ctcaggctgt ttacatgatt gtacgttctt tttgtaaagg atacagtgat
     3601 atgtatataa tggcataaag aaacgttcaa acaaaaaaac tcgtgaagca aaattaactt
     3661 cacgagttgt agtgcagatg atgggactcg aacccacacg agctaacgct cacatgaccc
     3721 tcaatcatgc ttgtctgcca attccaacac atctgcgtaa taataacaca ctaatgatag
     3781 cgaggtttta catagttgtc aagctcaaaa atgggaggac ttattaagat ggagaaatat
     3841 gacggagaat tttcaggact gggaatgatc ttaggtgtat tgattggact tgcattcgga
     3901 cgttttttgc ttggttttat gctaggaatc atttgtggaa ttgcaatgga ttgggcagcg
     3961 aatctatgga atgactatca tgacaattaa gttctcaacg cttgtttgtg tttttttaat
     4021 acttacgttt ttcctaaata agtggcaaaa ttttctagga tggagtatga tattgaaata
     4081 gtaaataaca aaaaatttgg aaagttctaa ctttttgaaa tgagggattg gctaagtgat
     4141 gagagaattt gaaaaatcca acaaattaga aggtgtcagt tacgatgttc gcggtcccgt
     4201 tctagaagaa gcggagcgga tgcaagaaga gggcattagt attttaaaat tgaacacggg
     4261 aaatcccgca ccttttggat ttgaagcacc aaacgaagtc attcgtgatt tgatcatgaa
     4321 tgcccgtgac tcagaaggct actcagattc caaggggatc ttttctgcta gaaaagcaat
     4381 tgagcaatat tgccaactga aacattttcc gaatgtgacg atcaatgata tctacaccgg
     4441 caacggagtg agcgaattga tcacgatgtg tatgcagggg ttgctggaca atggagatga
     4501 agtactggtt ccaatgccgg actatccgtt gtggacggct tctattgcat tagctggagg
     4561 cacacctgtt cattatattt gcgatgaaga ggcagaatgg tatcctgata ttgatgatat
     4621 caaatcaaaa ataacgagtc ggacaaaagc tattgttatc attaatccta ataatcctac
     4681 tggggcactt tatccaaaag aattattaga agaaatcgtt gaagtagctc gacaaaataa
     4741 cctgattatt tattccgacg aaatctatga tcgcttagtg atggatggtc tagaacatat
     4801 tccgattgct acattagcgc ctgatttgtt cgttgttacg ctgaacggtc tatccaaatc
     4861 tcatcgtgta gcaggatttc gagtaggctg gatggtccta agtggagata agtcacacgt
     4921 caaaggctat atagaagggc tgaatatgtt gtcctccatg cgtttgtgtt ccaatgtatt
     4981 gtctcagcaa attatccaaa ccgcgctagg cggttatcaa agtgtcgatg agctgctcct
     5041 gccaggcgga aggatctatg aacaaagaga atatatctat aatgcaatca atgatattcc
     5101 tggtctttca gccgtaaaac caaaagctgc tttttatata ttcccaaaaa ttgatacaaa
     5161 gcgattcaat attttggatg atgaaaaatt cgttttggac tttttacatg aacatcatat
     5221 tctacttgta catggtggtg gatttaattg gaaccagcca gatcattttc gaatcgttta
     5281 tcttcctaaa atggatgatt tgaaacaaac agctaagaaa atgagagaat tcctagcaac
     5341 ctatcgccaa aaatgatttt aaaaaccaaa tttttcagta ggagggccac agacaaacac
     5401 actgaggctc tctttctagt ggagttttct taaataacta aagtatatta tttagttgca
     5461 agtcttttct tgaagtttgc gacttcctaa agtaaaatac gaatagaatg cgaatacgca
     5521 tattttgggg aggatactta tggaaacgtt aatcgagtcc tttggaacga tcattaaaga
     5581 gattcgaaaa gaacaaaaaa tgacgcaaaa aatgctttcg catgatattt gttcacaaag
     5641 tgtattaagt cggatcgaaa ataatgagga actgccgaat gtattggtga tgatgcagat
     5701 ttgtcaacgg ctaggggtaa cgattgatca tgtgatgaat ctctctcgaa aaactactcg
     5761 gacaaacgat aaattatttg aactgttgga tagttgtttc caaaaacgtg attatcggaa
     5821 gttagaacaa gtactaaaat cttctgatat ttctgaaaat ctgtatcaag caactgactt
     5881 tcaacgctat tattattatc tcggtgtttg cgaattcacg ttgcgtcaaa atatctctca
     5941 gtccctttat tatttgaaag aagcattaag ctactctagc caaaaagaaa gaatccatgt
     6001 ttctgacaca gaaatacaac tgattagttg tatcggaaaa atatatggtg tggcaggaaa
     6061 aacggctgaa gcacgctact atttgagtcg aagcatccaa ctgctgcatc aatcagctga
     6121 tgaacgatca aaaagtgaat tttcgcaaat tttttataat tatggaaatt tcttattcca
     6181 tcaaaatgaa atcgacgaag cgttagaaca ggtgaatcaa ggaatctatt gggctcaaga
     6241 gaagaatagc tactattacc tcaatgattt atttgtgctt aaaagcttaa tatacaagcg
     6301 taaagaagaa ccagaaagag cagcattcta tgaaggctta gcacaagctg tgaaacaaat
     6361 ttcaaaaaat ttgtaaatgg tgtagaccat tcttgacttc ttctctaaat aagagtactt
     6421 tagaggtaga aaaacaaagg gagagaatag aatgagaact tttatctatg ctgacaaatt
     6481 ctttttagcg tcaggtgtca aacatgcagg ctatcttgaa attaccgatg gactttttgg
     6541 tgaatacacc aaagaactac ctgagggaga agtcaacatc attgaccaaa gcggcaaatg
     6601 gattgctcct ggtttggtag atacacatat tcacggctat atgaatcatg atgtaatgga
     6661 taatgatgca gaaggcatca aagtgatgtc tgaaggtctg ttatcttgtg gagtgacttc
     6721 atttttacca acaacattga cgtcaagcaa agaacgttta aaagatgtag ctgagacaat
     6781 cggcaaaatg tatcaggaag caccaggagc taaaatcaaa ggaatctatt ttgaaggtcc
     6841 tttcttcaca gaagaacaca aaggggcaca aaacccaagt tatttcgggg atcctgattt
     6901 ggatactttc catgaatggc aagaagcttc aggtggttta atcaaaaaaa ttgctttagc
     6961 accggaacgt caaggtgtag aagagtttgt caaacaagtg actgaagaag gagtagttgt
     7021 ttctctagga cacagtaatg ctacactaga agaagcgcaa aaagctgttg aagcaggcgc
     7081 aagtgtattt gtccatgctt tcaatggtat gcgtggattg aatcatcgcg aaccaggtat
     7141 ggtgggtgcg ttattatctt tagaacatgt cttttctgaa ttgatttgtg atggccatca
     7201 tgtacatcca aatgctgcgg gaatcctaat ggaaaaagca ggacatgatc atgtcgcatt
     7261 aattacagac tgtatgatgg ccggtgggat gcctgatgga aattacaatt taggcgaatt
     7321 tccagtagta gtaaaagaag gaacagcacg tctagaatct ggcaacttag ctggaagtat
     7381 attgaaattg aaagaagcaa ttaaaaatgt tgtagactgg ggattagcaa caccagaaca
     7441 agcagtcatg atggctagtc tgattccagc aatcagctgt aaaatcgatg atacatgtgg
     7501 aaaaatcgct caaggacgcg atgcagactt tatcgtattg aaccctgata tgacattgga
     7561 agcaacttat ctagatggcg tggaacgttt ccacgcataa tataatttaa gttaacctta
     7621 aaactaaact tcttaagttt ggttttaagg tttttttgtg ttctagtaaa aatcacttta
     7681 cataagtttc aaaagtttct ttaatttcct attgttcaat aagtaacaat gtaaagataa
     7741 aattaaaaat gttaatacgt attagcaaaa taaaagcttt ttttattgtg aataaatgaa
     7801 cataattagg aggagaaaga tgaacttatt tgacattttt cgatcgactt tgacagatat
     7861 gtcgattgtc agtgcaatca cttcaacagt ttttattata ttattagggt ttgtttgtag
     7921 aaaaaagggg attttttctg aatctgttgg aaaaattcta tcgaaagtcg tcttaacagt
     7981 ggctttgcca gcactggcat ttcgagcttt catgcaagat atcaatacag aattactgat
     8041 gcaaggaatc aatgtcttga tttggggatt agttatttat attattctta ttctagtttc
     8101 aaaacctttt ttcttaagct ataaagggga taagcaaaca acgttgcgtg tcctgacgat
     8161 ctttggttct acgacctttt tcggaacgcc tatcgtcagt gcgatttatg gtcctgtcgg
     8221 cgtgatgtat tcctcaattt tcaatattgg ctaccgaatc tttttgtatt cttatggata
     8281 cataaaaatg agtggcttga agatggaagc taaaaatatc aaagcgatgt tcgtcaatcc
     8341 aatcattatc gctacttttg ccggcctttt catttggctg ttccaagggt atctgccaca
     8401 agtgtcagtt acaggtgcag aaggagagat aagccaagta gcgtttttgc gaattgacca
     8461 gactgcaccg tggctatttc agccgatgga ttatttagct ggtttaagtt caccattggc
     8521 gtggctagca atcggcgcaa cattaggtga agtaagcttc aaaaaagctg catctgaaaa
     8581 aacttcatgg tattacagtc tagtcaaagt ggtatttgta cctgcattga atatcgtgat
     8641 tctagcagtt ttgacggcaa cgaatattct accagtcaat tttgaagcac tggcaacaat
     8701 cgttatcatg atggctacac cagctgcaac agtggcagca gcctatgcta tcagttttga
     8761 tagggaggca cttttagcct ccaatgcatc cttactgtca actatttttg ctgtggtact
     8821 gacacctgta tggatcgttg tattacaaat gattcatggc ataggtttat tttcataaga
     8881 aatggaggaa gaaagtatga gaatcgcatg ttacggtgtt cgtccgaacg agatagattt
     8941 ttttgaaaaa ttgaatattt accattatga tctcagtctt tatgaagaac tgttgacaca
     9001 tgacaatatc gtgtcagcaa aggaccatga tgctgtattg ttgaggggaa attgtatagc
     9061 agatgagacc aatcttgcaa aaatgcagga atatgggatt cgttatgttt ttacacggac
     9121 tgtgggagtc aatcatatcg atctacaagc ggcagctgat tttggtatga cagtagcccg
     9181 tgtaccatcg tattcgccaa atgcgatagc agagttatct ttgacttttg ccatgatgct
     9241 tttgagaaat actgcgtata cgacgatccg aacatctttc aaagattttc gagtggatga
     9301 gcaaatgttt agtcgtgaaa tcagaaattg cacagtaggg atcataggaa caggcaggat
     9361 agggttgacg gaagctgttt tgtttaaggg actaggagcc aaagtgatcg gctacgatat
     9421 tcatcagaca gaagctgcta aaaaagtatt gacgttccac tcgcttgaag agttattagc
     9481 caaaagcgat attgtcagta ttcatgtacc tcatatccca ggtgaaaacg atcaaatgat
     9541 caatgaagga tttttagctc aaatgaaaaa aggtagcatc ttgattaata cagcccgtgg
     9601 agagcttcaa gataatgaag ctattttaag agctttgaaa agcaatcatt tagctggttt
     9661 tgcaaccgat gtttttgcaa atgaaacaga ggtttttttc cgatccttca agccatggga
     9721 gacgattcca gaccctgcta tccagcaatt agtagaaatg taccctagag ttcttgtaac
     9781 cccgcatgta ggttcaaata cagatgaggc attatcaaat atgatttcga caagtttcga
     9841 gaactttcgt gaaataatag aaactggaaa aacgaaaaat gaggtatctc ttcctaaagc
     9901 ccgtcaatta agataagaaa acatggatat tcgtcaataa aatgtttaaa aatagtccaa
     9961 gtaaacagtt gttacttctg tatacttgga ctattttgta tgggataatt cgatgactca
    10021 acagtaatct gtgagctttt tccgcccatt ctttgtcaat tcataacagg ttagcggctg
    10081 gccgatttcc aaatatttga ttctttcgtt caatatatct tgttctacta aaaaattcaa
    10141 atatttcttg acggatatac gtgacagtga gcttttttga gccagttctt ttgttgaaaa
    10201 aatagtatct tcttttagaa taaatgcact tatttttttt aaagtcagtt ttgacagacc
    10261 tttaggcaaa tcgatgaatg atggtatttc gtgaatggga gtggtgtggt tgaatacttg
    10321 atcgatcaat ttctgatcgg ctttttcagt cgtttggatt gtctgagaga aagcaagata
    10381 cttttcgatc gccagctgaa aacgttgcaa agtgaacggt ttgatcaaat aatctaaggc
    10441 accgtagcgg atcacattct ttatggtttc gatatcatct gcagcagtga tcaaaatcac
    10501 tgggatatca tttccttgct gtctaagcca agataaaaaa tggataccac tttcgccagg
    10561 catgtataca tcaagcagca gcagatctgc gtttttttgt ctgaaatatt tttttccttc
    10621 ttctacattt ctgaaacttt cgattacgtt catattaggg atgctttcca caaaagtacg
    10681 attcaattct gcaaccattg gatcatcttc gattatcagg acatttgtca tcattttgat
    10741 cttccttttt tggtaattct agatagacga ttgtaccgcc atttgtttgt ttggaaaatt
    10801 ctaaaaatcc accgtggcgt ttgacgattg aatggatgag attcaatcca aacccacgat
    10861 tgattccctt cgtcgaaaaa cctagcgtaa atattttttc tgagatttct tctgatatcc
    10921 cgacaccgcc atcgcagacg gtcacgatga atacttcagc ttctgtgtca tagtttaaat
    10981 gaatccggac ctgtccgttt ctttcatgtt tttgtagcac ggcttctttt gcattatcaa
    11041 gtaagttccc taacacttgc aaaagtgaat ggatggagtc atccatctcc attttaggga
    11101 taaaagaatc gctttctaac agcatttcaa cagattgttc tttggcttcg tttattttgc
    11161 ctaataaaaa accggcaatt gcaggtactt tgatcagact tgagacttgg ccaatctctt
    11221 ttttataatt ttgatttagc tgctggataa aatgggtgac ttcttcatac ttcttcaaat
    11281 caatcaaacc agaaatgaca tgaagtttat tcatgaattc atgggtttga gctctcatgg
    11341 agtttatata ttgtttcgtc cccccaagct cctccattaa tttcattact tctgatcgat
    11401 ttcgtaaagt tgcgactgct cctaaaaaac ggtcattgct aaaaagaggt gtaacgctag
    11461 aaatagtttc tatcccattt aaatacatgg attgagtccg aacggtcgtt tgatgctcaa
    11521 tcgtctcttt aaacaaaacg tcatatagat tatcactgat ttttttccta cttcgaaatc
    11581 caatagctgc tctgtttggt agaaaatgtt ttttgcaggt gtattgatca agaggatagt
    11641 tttatcagaa gagatggcta agattccttc atctacttca ttttcgataa tcgttttttc
    11701 acttaactgt tctgctattt cttgtggttc aagtccaagc agtacttttt tcattttttg
    11761 ggcaagaaaa acggcgccta tgatgcctac gctaagtcca ataatcaatg aaaaaacaat
    11821 cattttttgt ccatctttga cttgattgtc aatcgtgttg tttgttaagc caacacaaac
    11881 tgcaccaata atctcttgat cataataaat agggacgaaa tagcggaagc cgaaccctaa
    11941 cattccttct ttttcagaga aaaattcttg tcctccaatc acttttttca catcttcatg
    12001 acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga gaatatcgaa tgccttcagg
    12061 gtccatgaca acgataaaat ctaaattcga tgcaatcatg atttcatttg ttattccttg
    12121 aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg atgagtttat cattggcaac
    12181 gattttagca atgctagaga ttttatctga cacactagat cggttcatcg aaagaataaa
    12241 attcctgatc aatatggcag aaacaatcaa agaaacgaat gtggagaaac aaacgacaag
    12301 gatgatcgtg ttctggagct ttaatggttt tttcttaaaa aaaggaaaca taggctacct
    12361 cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa atttaacaaa ttgtcaacga
    12421 taaacacaac cttcctataa aaaacaccac tgagtcggta aaagaagcta tacagaattt
    12481 tcaaacattg gtatgataga cgtagaaatg gaaggaggcg tgtatatgat agggggaatc
    12541 gcacttgaga cgatttattg gtatacattg ctgatttgtg caggattggc agtcctattg
    12601 attatttttg gagatgtatt cgattttgat ggcccgcttg atccgatgct tattatccct
    12661 tggttaactt tcacaagctt atttggtttt ctaggtgagc gtttaacaga tcaatcatct
    12721 ttatttttat ttattgctag tggaggcata tcgatagtgt tggtattttt gcttaatttc
    12781 tatatattga tgccgatgaa acatgccgaa tcaacgatgt cgtcttctga aaaaactctg
    12841 gaaggacaag tagctgttgt gatcacaccg atccctgtac gaggtatggg agaaattcag
    12901 ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt tttacagccc ccaagaacag
    12961 acagctgagc ggggaacgaa agtattgatc attgagatca aggaaagaat ctgttatgtc
    13021 atgccttatg aaagcatgat aaaagcttaa aggagcgtgt gttaaatgga aataccagtt
    13081 gtttttatcg cgatcgcaat cgcagctttg attttactga tgttgctgat cgtctttgta
    13141 gcgaagtacc agacagcaaa accggatgaa gcgttgatca tcagtggaag ttatttagga
    13201 agcaaaaatg tgcacattga tgaaagcaat aacaaaatca aaattgtgcg tggaggtggc
    13261 gcattcgtct tgccagtttt tcaacgttcg aatcggatca gtctattatc gagtaaattg
    13321 gacgtctcca cccctgaagt gtataccgaa caaggcgtac cggtgatgtg tgatggtact
    13381 tcgatcatca aaatcggttc atctgtagaa gagattgcta cagcagcaga acagttttta
    13441 ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag tattagaagg acacttgcgc
    13501 tcgattcttg gttctatgac tgtagaagaa atctatcaaa atcgcgataa gttcagtcaa
    13561 agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg ggttgatcat cgtttctttc
    13621 actatcaaag aggtcagaga caaaaacggt tatcttgact ctttagggaa accaagaatc
    13681 gcccaagtca aaagagatgc agatatagca gaagcggaag ctctaaaaga aacgcgtatc
    13741 aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat tacagcgaca aacagagatc
    13801 gcagaatcat taaaagaaaa agaactgaaa ttagcaacct acaaacaaga acaagatgtt
    13861 gctaaagcaa aagcagacca agcctacaat ttagaaagtg cccgagcaca acaacaagta
    13921 atcgaacagg aaatgcagat taaaatcgta gaacgccaaa aacaaatcga actggaagaa
    13981 aaagaaatca cacgtcgtga aaagcaatat gattcggaag tgaagaaaaa agcagatgcc
    14041 gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag tcaaagaagt gactgaagcc
    14101 gaagcagaac gattccgtgt ggaagcactg gcagaagcgg aagccaataa gacacgttta
    14161 gctggacaag cagaagccga agcagcacta gcaaaaggaa aagcagaagc cgaagcaaaa
    14221 caaaagatcg ccaatgcctt caaggaatat ggcgaagcag ctgtcttgag tatggttatc
    14281 gatatgctac cgcaattgat gcgcgaagca gcacagccgc taggaaatat cgaaaaaatc
    14341 tctgttgtcg atacaggaag cagttcagga gaaaccagtg gagcgaaccg agtgacaaat
    14401 tatgccacaa atttgctttc ttctactcaa gaaacactaa aagcgacaac aggacttgac
    14461 ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta ctagccaagt aaatatgtat
    14521 gaagcgccaa aagaggcaga acaaaactcg atgaaggaag aataaagtag tacacttcac
    14581 cgttcaatca aaaatggagg aagacttttt aaagttcttc tctccatttt tttattttat
    14641 tgcaaaagaa aattagtcct cgctattttt cttttgtttt tggatcttag cttctatctc
    14701 ttgtaaattt gttcttagca atcgttcttt cttttggagt tcgaccaaat cttctgatag
    14761 ttcttccttc ttttctgttt caggaggacg aagaaagaaa ttgataccga ttgcaatgaa
    14821 tgtccctata aaagtatcta gtacacgttg gatagcatac aaagcggatt cgccttgagg
    14881 gatgctcaac gcaatcagca acaacgtagc tattgcggaa atgataccgg aattattgtt
    14941 aataccatct gacacgacga tcacaataat aactagaata ggaagcaaga tcaattctac
    15001 caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa atgatagcta atgtaccacc
    15061 taatgtattg ccgatgatcc tagactttcc aaaagaaacg gttgtagtca agtcctgacg
    15121 caaagaaaaa acagcagata atgcagcaat catcgggatt cctcgttgag tcaaatgaaa
    15181 aaacaagata cataaaaaaa cagctaaggc acttttgatc gtacgcatac ctagtcgaaa
    15241 acggccaaat tgcatagaga ttctcctttt tggtcttttt actatcatac ctaaactttt
    15301 gtctgatagc tagtttttca ttattcaagt ataattaaaa taaaaaaggg ggaagtaaat
    15361 gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc ttcttttcga tggtcacagc
    15421 gggtagctta tcgctgttgt attggccagc tgcagggatc attgcattat taagtgtagg
    15481 aaataccaag cgctctacat tgcgtacggg catgaatcga atcgcagcct ttatattagc
    15541 tacggcaaca gcactgttca gtttttttgc tgttgggtat actatttttg gcttcagtct
    15601 ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa ttaaccgaag ggatagtagt
    15661 aaattcggtt ttggtcactc attatttagt ggaggaaaac atgtcttggc agctgatcgg
    15721 caatgaagcg ggattaatgg cgattggatt agtatttgct ttgcttgcaa atgtttacat
    15781 gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag atcgaaaaag aatttcgaaa
    15841 tattttgaga caaatggcag aatttctttt atcagaaaat aaagaagatg tgcagatcaa
    15901 atgcgaacat ttactgacat tcattcgcga aagccaagaa gatgccagag aacatcaaga
    15961 aaactactgg ctcagacagc cgctatacta cgaaacttac ttctctatga gaagagcaca
    16021 ggccaatgtg attaaagaca tgctggaaaa tctagagaga atccagcaac cagcttatta
    16081 tggaaaacat atctatggtt tattgattta tactgcagaa acattttcag agtcaaatga
    16141 tggcagacaa attttaacac gtattgaaga agtttatgta ttgtatcggc aaatgcctct
    16201 gccgactagt cgccctgaat tcgaggatcg agcagaattg ttccagtttt tgcagtcttt
    16261 taaaagtttt attgaaatca aggcagaatt ctcccaacaa aaaatccaaa aatagaggtt
    16321 gtgaattcag ctaatattca ggtcaaaaag ctgatttcac aacctcttct ttaaacggtg
    16381 ttctcctgtc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt
    16441 ttgaaattgc tccttgtgac tcggagctga acggtaggct cacaacctct ttcttaaacg
    16501 gtgttcaaaa gctttcctct gcggtaataa gtccagccaa acgaaaaata ggaggagcta
    16561 tttttgtttg gctgttctta ttacttgatg cagaacagct ttttcacaac cttttaaaat
    16621 tacatatttt tccatagaac ttgcatttga tccaagattt cgatgacatc ttttgtcaag
    16681 aagagagagc gattgggcaa gtcaccttca atcatgtgta ccatattctt tatttcgtaa
    16741 ttcattgctt cagatgtatt cccgctttcg atgaattcct ttgtaccatc tgtaaataag
    16801 atctccgcac gatcagcacg cgggtaatca gcaatcgtca cataaccgtt ctcaaaggca
    16861 acgataccga cttttggcat ttttgcctga aaagtcaagc tgacagcagc aagttcattt
    16921 tctttgttac gcaagatagt gacggattgt tcgtccacgc ctgtttcaaa aggaaccatt
    16981 gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac gagcgaaaga tacagcatac
    17041 gtaccgatat ccaacagagc tcctccagct aattctggat tgaagaaccg gtttttaggg
    17101 tctggttctt tgtaactgcc gaaaggtgct tggatcattt ttaaagcccc cagttttcct
    17161 gtatccatta ttgagcgtaa ctgctgataa agtggcatgt tgaagattgt catcgcttct
    17221 gctaagatca aattcttttc ttctgctaag cgcatagcgt cagccagttc ttttttattc
    17281 atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt ccaatgctgc caagatatgg
    17341 tcgatatgct gtctatttgg tacagcaatg tacacgatgt cgatttcagc atcgtctaac
    17401 atttcttgat agcttccgta agcttttgga atattgtaac ggtgagcgaa gttttccgct
    17461 ttttctgaag ttctggaagc aactgctgtt agttcgctcg tctcttgatt gaagctttcg
    17521 gcaaactcat gtgcgatggt accaagacca acgattcccc attgatattt tttcatctga
    17581 aaacgctcct tcatagttgg attgagtttc ttgtctgtct attattataa caaaaatcac
    17641 gaagaataaa gaggagagga cttgaaacat taaaaaaaat gaaagcagcg atttaagagg
    17701 tgctttttga tcaaaactta agtagaatag ttaaaaggag gggataaacg tgcatcaagt
    17761 atatattgat cttattatga gtgtattatt agaacagttt gaaactgaaa aaaatttttt
    17821 agatgaatac ttgctgatca gtccagaaca atggcaaaaa tggaaagatg gcgagaaaaa
    17881 tctgccatct gaagcgatgc agaaagtaaa aaatcttttc actgattatg aatggatgct
    17941 aacgcaaaag attttgcgtc aaacgattat ttttcccgaa aaacgaaacg ttgccgttgc
    18001 agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg cttagcagca aagcagcaaa
    18061 agtcgagctg atccctttga aaaataatca gcaggctggt agataccttg acttgaaagt
    18121 aagtattcaa tatgatgaat ggggctatga tgatattttg aattttcgtc tgccagcaga
    18181 tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc gattgggtga atgaaaatct
    18241 ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa gaacaacgaa aggatttttc
    18301 aagtgaataa atttttagga tttggagcag ttgtgattat gatcgcagca atgggctatg
    18361 ttatttttag cgatgatgat tcgcatgttc aagccggcaa tcaagcagtc gaacgaacaa
    18421 gcaccaaaac aactgctagt aaagaaaaga acagtcaaac aaccagcagc aaggtaaagg
    18481 acaaagaaac aaaagatcgt acgtccaaac aattgccaga tgttaaaaca gacgattgga
    18541 agctagtttt ggttggtcca gatcacaaat tagaaaaaga aattgatgag tctactcaat
    18601 tggcatcatt aagtaacgga tacttgatcg acaaacgcat tgctccagaa tatgaagcat
    18661 ttgccaaggc tgctgaagca gctggatttc cattagtgat ggtctcagct taccggtctg
    18721 tatcatccca acaacaagtt ttcgaacaaa atgtccaaga tgtgatgagt cgtcaaggtg
    18781 tatcagaaga ggaggcgaca aagattacta aagaaacgat tacggagcca ggatacagcg
    18841 aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa ctggtacaac agttatcctt
    18901 ctactgtatt ggatgcaagt tacggagatc agcctggagc taaatggatc gcagataatg
    18961 ctgcgcagta tggatttatc gttcgttatc caaaaggaca cgaagatata actaagatca
    19021 catatgaacc ttggcactta cgttatgtcg gtaaagaaaa cgctgagtat atcaccaaac
    19081 accaattgac gctagaagaa tatctgaatg aattaaatga aaagtaggaa ttgatcatgg
    19141 cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg ttggccagca gtggttgccg
    19201 gtgtagcagt gatactctta gcctttgttt tttccttgaa gaatctgtct tctcctttta
    19261 ctgataatca gcaagaaaat gaagcgcaat cccatcagca atttattgaa cggcttgttc
    19321 cgcatgccca agaactacaa gatggttatg gagtgcttcc tagtatcatt ttaggacaag
    19381 ctatattaga atcaaattgg ggaaaaagta ccttggctag caaatacaat aatcttttcg
    19441 ggatcaaggc ttatggggat cagaagaaag taagcctgga aacaaaggaa tttgttaacg
    19501 aagaatggat aactatccaa ggagacttca aagtgtatga ttcatgggaa cagtccatgg
    19561 atgaccatac acaactgttc gtccaaggag tagattggaa tcctgcgctg tatgaaaaag
    19621 tgattacggc caccaactat caggaagccg cacaagcatt gcaagatgca ggttatgcga
    19681 cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga gacgtatcaa ttgaatcagt
    19741 atgaccggag atagcaaagg agaagtggct gatggcagaa caatttacgc cagaaatcat
    19801 gacggaattg agaaaagata tcgtaagagt accggaaatc attttagaag caagcgggat
    19861 caagatattt ggaaaattga tccgttcgat catctttact acggatattg cgatcatacg
    19921 taatacaaat gcgaatgcag taattgctgt gtatcctttt acacctcatc cagcgattac
    19981 taaaagcatt attgaggcag ctgacattcc agttttctcc ggtgtaggtg gtggtttgac
    20041 acaaggtgct cgttcttcct acatgagcat gttcgcagaa gcgcaaggtt cgttaggggt
    20101 cgttttgaac ggtcctacgc ctttagagac agtaaaagaa gtttgtcgag tagttgatat
    20161 tccagttatc agtacggtaa cgtcgaaata tacagaaata gatgaaaagt tagcagctgg
    20221 tatttcgatt atcaatatca gtgcaggtaa agaaactgca gaagcagtac gccattttcg
    20281 taaaaaatat cctgaacttc ctatcattgc gacaggtggt ccaacggaag aaagtattgc
    20341 tgagaccatt caagcaggag caaatgccat cacatatacg ccaccaagta gcggagagct
    20401 ttttagtcgt aaaatggaaa aatatcggga aactgagaaa aagaatcatg aataacaggt
    20461 gagaatcaaa agacgaggaa gagcagatag tggctcttcc tcgtcttttg tactccagta
    20521 ctatttgttc agaattcaaa aaaaagagaa atttttttgt ttttttacct gtaatgtaca
    20581 taagaagagt taaaaccttt tgttattatt agcattctaa caaaaaaatg tcttgattta
    20641 ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta aaaaaggcac ccgacaattt
    20701 agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga actgaccaat caagttgcca
    20761 tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat ttcatacaga attactagtt
    20821 atttttctgt tttttaggga agagcgcaca cgaagaagca tgcttattta gctatgtcaa
    20881 ggatattggg cagttgtctg atatcctttt ttctttgcgt aattatcctc ccatcattga
    20941 agcaagaagt gtttttagcg atgaaagaag aatcagcact atctaatttt tcttgaaagt
    21001 attgagttgt tcatcctagg cagaacgata aggcaagtct tggtttttat tccccttgaa
    21061 aaagatcgtt taataaatat gttctgtata aaaatacagt atattgaaaa ataacaatgg
    21121 aagtagaggt gcagagaatg aagaaaagaa tgagaatcat cttagcttta agtgttatgt
    21181 tattcttctt gacggcttgt gggcaagaag atgacaaaaa aatcacgctg aacatggctt
    21241 ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt aatcgagctt tatgaagaaa
    21301 aaaatcccca tgtgacgatc gattatgaat tttatgatta tgaaggatat tacacaaaat
    21361 taggtacact agcagcatca gatgatatct gggacatttt ccaactgaac aatcaatttc
    21421 ctcaatacat caatcagatt gaaccattag atgaatatgt ggagcaaggg ataattgata
    21481 tttctaatgc agatgaatcc tatttagcaa cgacgactta cgacgggcaa ttagttggac
    21541 tgtctaacgg ggtgaattcg tttgcgattg cttacaataa aaatatattt gaacaattaa
    21601 atatccctga accagcttca aattggacat gggatgaatt tgaacaaatc agctatcaaa
    21661 tcactgaaga attaggaatc tatgctattt caagattcga agattttata gctggctgta
    21721 ttattcaaat accacaagtt gagaaaggat tgaattttta taatcgggaa gatctatcta
    21781 acagcttggg atttgaaaat ccagactatc taacagattt tttccagctg cgtaaaaatc
    21841 tggtagacaa aggggcccat ccagaaccag gtggtggagc tgcaagtacg acggatctgt
    21901 cttttcaggc ggttagggat agagaagcgg caatggtgtt tctatcaagt aaccagttga
    21961 cagaaatatt gtcaggggct cctgaagata tggagattca gttgattaat ccacctcgca
    22021 gaaaagcaga tggagaatct ggtatccctt tacgttcttc gcaaatgctg agtatggcaa
    22081 aaagctcagc ccataaagaa gaagctgcta agtttattga tttctttcaa aacagtgaag
    22141 aagccaatga aattttaaaa ggagaacgtg gtgtaccaat catgtcgaat attcgagaac
    22201 agctcgctcg tgcagatgat gcaactttgc aagcgacatt tgattattta gaaatgattt
    22261 ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc tttgaatccg gaaattcaag
    22321 accaatacaa tcttttagtt gaaaaagtca tttatgaaga agtaacagca gaaggaaggc
    22381 atgaaaaaag aaaaactaaa acggttcttg gatcaagaca atgtggtcgg ttacgtattt
    22441 gcttctccat tcatcctagg atttctattg ataacagtga taccgatgat cttgtcactt
    22501 tattattcgt tcaccgatta tcgtttggga aatccagtct catggatcgg cttggataat
    22561 tatataaaga tgctgagtga tgcccgtttt ttgaactcca tccgagtcac ttttctctat
    22621 gtatttgcat ctgtacctgt gaaactgatc tttggtctat tgattgctta tcttttaacg
    22681 aggaatatca aaggaacgac tttctttcgt tctttgtatt atgtgcctag tttgatcggt
    22741 ggcagtattt ctatcgctct agtttggcgg gagttgtttt ctaaaaacgg gttagtgaat
    22801 attttcctgg aacgattagg cgcacctgcg ctttcctggt ttagcgatca aaaactggtc
    22861 atgatccctc ttgttttgat gtctgcttgg caattcggtt cggcaatgat catctttgct
    22921 gcaggtatca aagagatccc aggctcgtat tatgaagcgg cagagatcga tggagcgaac
    22981 aaatggcaga cgttttacaa gatcacgtta cctcttcttt cgccgatcat tttgtataac
    23041 ttggttatgc agacaatcgc ggcgtttatg acttttacgc aggcatttat cattacccaa
    23101 ggtggtccga atgatgccac gaacttttat gctctttatg tgtataacca agctttttct
    23161 tttaattcta tggggtatgc tagtgctatt tcgtggatca tgctgttgat tatgggagct
    23221 gtcacctttc tgattttcaa atcatcaaaa aaatgggtcg ttgtgtgaat gagaggagga
    23281 agaaatgaat aatacagtaa cgaaatggtt tcaatacggt tttattttgc tattaggtct
    23341 agtcatgatt tatccggtct tatggatgat cgcaggttct tttaaaacgg atcaagagat
    23401 tatgagtggt tcgctgaatt tgattccaga gacttttcaa tgggaaaatt acgcaaaagg
    23461 atgggcgggg tttgctggaa tttcattttt tactttcttt aggaattcgt ttgtcatctc
    23521 tgcagtttcg acgataggga cagttctgag ctcgacttgt attgcttacg cattatcgag
    23581 aatcgatttc aaaggaaaaa gattctggtt cgtcgtcatg ttagtaacga tgatgattcc
    23641 tgcacaagtg attttgattc cgcagtttat tatttacaat cggctgaatt tggttgggac
    23701 gtatgtgccg ctgattttac cacatttttt tggacaagca tttttcattt accagatcat
    23761 gcaattcatg gtcagcatcc ccaaagagtt agatgaatca gcgattattg acggatgcag
    23821 caagtatagt gtctttacca aaattatttt tcctttgctg aaaccttcga tcatcacgac
    23881 gatcatcatc cagttttatt ggaaatggga cgacttcatg ggaccattga tttatttaaa
    23941 caagccgaga tcttacacgg tatcgattgc catcaaatta tttgcagacg ctggatctag
    24001 tacgacttac gcgagtatgt ttgcgatgtc tacgttgtcg ctgctaccgg tttttctgat
    24061 tttcttattc ttcaataaat atcttgtcca ggggatcagt acaagcggtc tgaagggctg
    24121 aatatgaaaa agccaataga agagatgttt ctttacaagc tcaactacta tgtctgtcgg
    24181 atttttttaa ccagtcttat cagtaatgct cttttcgctt tagttctagg atgtttgttc
    24241 actttaggat tagtgctgtc tgctcgtgtt gtctatctga tttatttttt gatgatgtat
    24301 ccttcttatt atctatctgt ccattacttg gcagaaaaaa aggtatgttg gaggaaaggg
    24361 aaaatcaaac ggatgtgttt catcattacg ttgtttcagc tgtttgtcat tatttttgca
    24421 gttgattggt atttattagt gggaaagatt ggagcttggt atctgattcc ttttctattg
    24481 tgtctgttgc tttttgtatc ccatagtttt tttagtacgc taattgatat ctgcaaagaa
    24541 gataaagcag atagtcttgt ttatttgatc aaaaggaata tctggctgtt gtcaaaaaac
    24601 gtcaaggggt ttctgtggta tttatgcctt ttgtatggac tgttctggtg tatacagcct
    24661 gattttttac tcactgcatt actgtatcca gggctgccat ttgtcacagt attttgctta
    24721 aaagaaaagg agaatgactt taaatgaaga aaaaatatgt acaagtcgga cttggtggga
    24781 gagcgcgttt tttttatgag gcaattgcct ctacgtatca tcaaacttgt gaactagtcg
    24841 gtttttgtga tgtatctcaa acaaggatgg attatgcaaa tcggatactt gtgaacaagt
    24901 atggacagaa aaaagtaaag acatgtatgc catcacaatt tgagcaacta ctggatgacg
    24961 tacggccggc tgaagtcatt attacgtcag tagatcgaac gcatcatgat tatatcattc
    25021 gtgcaatgaa aaaaggatat gatgttattt gcgaaaaacc tctgactatc gatgaggaaa
    25081 aagctcaaga aataataacg gtccaaaaac agacgggaag aagcctgcga gtcactttta
    25141 attatcgtta tgcaccgtat cacacgaaga taaaggaact gatagaaagt gatgtgattg
    25201 gtgatgtgtt ttctatccac ttcgaatggc tgttaaatac gcaacacggg gcagattatt
    25261 tcagaagatg gcatcgaaat aagcacaata gcggcgggct tcttgttcat aaatctaccc
    25321 atcattttga tctagtgaat ttttggttga acagccgtcc ccaaaaggta tttgcattcg
    25381 gtggattaaa cttttatggg atgcataatg cggagaaacg cggagtagaa atattttaca
    25441 gtcgatgcta tggaagcgaa ctagctaaag ctgatccatt tgctatagac ctgtcagcca
    25501 atgaggaatt gcgagaaatg tatttagaag cagaaaaaga cagtggatat attcgtgacc
    25561 gcagtgtatt ttccgatgat atctctattg aagatacact aggcgtaacg gtcaaatatg
    25621 aaactggtgc gattttgacg tattcgctaa acgcgtatat gccgtgggaa ggattcaatg
    25681 tttctttcaa tggcagcaaa ggacggatcg aaatgaaagt gatcgaaaag tcttacatca
    25741 atgcaggtgg agataaaaag gatgaaggag cagccgttct tcagtccatc catgtctatc
    25801 cgatgttcgg tgagccatat gaggtaatcg tcgaacaaga agcgggcgga catggcggag
    25861 gagatccgcg tttattagat gatctatttg gtgaagaaaa agaagaagat ccgtggaatc
    25921 gggcagcaac tcacgtagat ggtattttgt cgattttgac aggaatcgca gcaaatcatt
    25981 ctattgcttc aggaaaagca gtagtgattg acgagcttgt cagcttaaaa gaataagaat
    26041 agatttacgg aaattacttc ccgtagttta taaaatcttg ccttattgcc gataggctgt
    26101 cttttgaaca ctatttattc ggaaaaaggg gctatgacat gctttttgtc atgccttctt
    26161 tttatgtggg ctgctttttt agataggaga aaataaaaat aactatttaa acccgaacaa
    26221 aaaggaataa tgcattaaaa atcattagat tttacaaaaa aataagtaaa ccacgttgtc
    26281 agtttcagaa agtagtgtta gactgttttc aatatttgaa acaacatttt ttattcaaat
    26341 atggcaaact gccttcacgt gcaaaacagt tgcaagaaaa tgaatttaca ctattattag
    26401 ttttacatac ctacttatat atcgtcataa tatggatgac agtttctagc cagtaccgta
    26461 aatgctggac tataagtaaa agattggcta tttcaccctt cttttcttat gataaagtgg
    26521 agggtgtttt tctttaacag aaacatagta ggtttttgca caaaagcggt tatttcggta
    26581 ttaataaatg ataaaggagc aaataaagtg aaagaattag ttgaacgtat tcaaaaagac
    26641 ggacgtatat taggcgaggg cgtattaaaa gtcgacagct tcgtcactca ccaagtagac
    26701 cctgtattga tggaacaaat gggaaaacgg tttgcagaag tatttaaaga acaaggaatc
    26761 acgaaagttg tgaccattga agcttctggg atcgcaccag ctttatttgc tgcaagagaa
    26821 ttagatgttc caatgatctt cgctagaaaa gcaaaaagtt tgacgatgga tgaagaatta
    26881 ttgacagctt ctgtttattc ttttacgaaa caagtgacaa gtacgatttc tatttctcgt
    26941 aaatttttat cttcagaaga caaagtattg attatcgacg atttcttggc aaacggacaa
    27001 gcggcaaaag gattgatcga attatgccag caagctggag cgcaagtaga aggaatcggg
    27061 atcgtcatcg aaaagtcttt ccaagatgga cgtcagttac ttgaagaaat gggattgaga
    27121 gtcgtttctc ttgcgcgtat tgcttccttg acgaatggac aagttgagtt tcttgaggag
    27181 gacgcataat cgtggaaaaa gaaacacaaa atggaaaagc tgcggtttta gggctgcagc
    27241 atctgctagc gatgtatgca ggagcagtag cggtgcctct tttgatcggt acaggactag
    27301 gctttaatca agaacagatg acttatttga tttcaattga tatctttatg tgtggattag
    27361 cgacattatt gcaattgaca gttaaccgat ttttcggaat tggcttgcct gtcgttttag
    27421 gatgtgcgat ccaagcagta gctccactga tcttgatcgg aaccaatgaa ggagtaggag
    27481 ctatctacgg atctattatc gcttctggga tctttgttgt gttagtttct ggtgtctttt
    27541 ctaaaattaa aaaactattc cctcctatag taacaggaac ggttatcacc gttattggtt
    27601 tgactttgat tcctgtagca atcgagaaaa tgggtggagg taatgcaaca gcagctgatt
    27661 ttggtgacaa aacaaatctg ctacttgcat ttgtgacgat tctcctaatt gtaggcgtgc
    27721 aaatgctggc aaaagggttc attcgttcaa tcgcggtatt gattggttta gtcggaggaa
    27781 gcatcttagc tgctttcatg ggaatgattg atctgagcgc gattggagat gcgccgttgt
    27841 tccatgttcc acaacccttt tattttggta aaccaacttt cgacgtatgg tcgattttac
    27901 taatgatcat catttccatg gtcagcatgg tagaatcaac aggtgtatat tttgcacttg
    27961 gtgacattac tgggaaaaat gttggagaag aagaattgaa aaagggatat cgtgcagaag
    28021 gacttgccgt tatcttaggc ggtattttca atacattccc ttatactggc ttttctcaaa
    28081 atgtcggatt ggttcaactt tcgggaatca aaacaagaaa acccatctat ttctctgcat
    28141 tcttcttgat tgttttagga ttattcccta aaatcggtgc gttagcacaa atcatccctg
    28201 agccggtact tggtggagga atgttagtca tgttcgggat ggtggcagtc caaggaatcc
    28261 gtatgctgtt ggaagttgat ttcagcaatg ataaaaatct attgattgtt gctgtttctg
    28321 ttggatttgg gctgggtttc aatgttacgc cgacattatt cagtcaaatg ccgcaaacat
    28381 tgcaaatgtt cactggaaat ggcatcgtaa tgagtagcat tacggctatc atattgaatt
    28441 tgatctttaa tggattgaaa aaagatgaag aaatattgga agaagccttc aagaagtaat
    28501 agagcaaaag aaaggttgtg aaatcagcgt tttgacctga ttgttagaga aacaaacgga
    28561 aaaatagttc ctcgtatttt gccgtttgtt tgagctaata ccgagggtca gctgattgaa
    28621 caccgtttat ccataaaaag gaatgtgacc taacttttgt cacattcctt tttttcatct
    28681 ttaataaaca cgaacaataa aatgtttttc tttgttaaca ttcgaaattt ctttgacatc
    28741 aaaaaaagtg attgttaaaa tatgaatgta gaaaaactgg gaggaaaaac aatgacagca
    28801 aaaatctctg tgattatggg gagtacttca gattgggaaa caatgaaaga agcatgtcaa
    28861 atattggatg aattcggtgt gtcttatgaa aaaaaggtcg tttctgctca tcgaacccca
    28921 gatttgatgt ttgaatatgc acaaaacgct cgaaaaaaag gaatcaaaat cattattgca
    28981 ggagcagggg gagccgcgca tctgccgggt atggtcgcat caaaaacaac tttgccggtt
    29041 atcggtgtac cggtccaatc acgaacatta aatggtttgg attcgcttct ttcgattgtc
    29101 caaatgcctg gaggagtacc tgtagctact acagcaatcg gaaaagcggg agcaattaat
    29161 gctggactac ttgcgataca gatgctttcc atgtatgatt tagaattgga ggctaaattg
    29221 gctgaaagac gttcaatgtt ggctaaaact gttatagaaa gtagtgatca acttggttaa
    29281 acctttgttg cctggttcaa cgatcgggat cgtcggtggc ggtcaattag gtcgtatgat
    29341 ggcgattagt gcaaaagaaa tgggctttaa agtaggtgtg ctggatcctg tgagcgattg
    29401 tccagccgca caagttgcag attggcacat tgaaagtgtc tatgatgaca cctttgcgct
    29461 gaaagagctt gccagaagaa cagatgtcat tacctatgaa ttcgaaaacg tcagtgtaga
    29521 ggcgttaaat gcgattttac ccatgtcatt tattcctcaa gggacagact tgcttgcgat
    29581 tacgcaagac cgcctgttag aaaaatcttt tttggaaacg aataacatcg tgattgctcc
    29641 ttatgcaacg atcgtcagtc cgacggatat ccaagatgcg attgacggga tcggctatcc
    29701 ttgtgttttg aaaaccactc gaggcggcta tgatggcaag gggcagtatg ttttgaaaag
    29761 tcgggcggat ctagctcctg ccatggacct tttaagagaa ggaacctgtg ttttagaagc
    29821 atggatcccg tttgaaaaag agatttcgat catggtggca ggaaatggtc aaggagattt
    29881 cacgactttt ccggtcgtgg aaaacatcca ccacaacaat attcttcatg aaacgattgc
    29941 tccagccgcg attgatcagg atgtgatcga ggaggctgag cgtattgccc gtgtgattgc
    30001 agaagcagtg agcctttcag gtgttttagg ggtagaaatg tttttaacaa aaacaggcgg
    30061 gctatatgtg aatgaattag ctcctcgtcc tcacaattct ggacattatt ctattgaagc
    30121 ttgttctatg agtcagttcg atgcccatat tcgaggtatt tgtggttggc cattaggaga
    30181 aaaagtacgt ttgataagca atgcagtaat ggtcaatgtt ttgggagaac aactgctgga
    30241 aagctatcgc ttgattgaga ataagccaga ctggcaattc catttctatg gaaaaaaaca
    30301 agcaaaaaaa ggtcgcaaga tgggacatat cactatcttg acagaagaca tccagcaaac
    30361 gctggaagaa atcaaacaaa cgaatatttg ggattagagg agaaaaaaca tgttagaacg
    30421 ttatacacgc cctgaaatgg ggaatatctg gacggaagag aacaaatacc gtgcgtggct
    30481 cgaagtcgag attttagcag atgaagcttg ggcagaactt ggtgaaatcc cgcaagaaga
    30541 tgtgaaaaaa attcgtgagc atgcatcatt tgatatcgat cggatcttgg aaatcgaaaa
    30601 attaacacgc catgatgtcg ttgcgtttac acgagcggtt tctgaaacat tgggagaaga
    30661 aagaaaatgg gtccattatg gtctaacaag tacggatgtc gtagatactg cttatggcta
    30721 tctcatcaag caagcaaatg atattttacg ggaagacttg aaacgtttta cggaaatcat
    30781 tggcgaaaaa gcaaaagaac acaaatatac agtgatgatg ggtcggacac acggtgtcca
    30841 tgctgaacca actacttttg gattgaaact tgccacatgg tattcagaaa tgaaacggaa
    30901 catcgaacga tttgaacatg cggcaaaagg agtagaagca ggaaaaatct caggagcagt
    30961 gggaacgttc gcaaatatcc cgccatttat tgaagagtat gtatgtaaac aattggggat
    31021 ccgtccacaa gaaatctcta ctcaagttct ccctcgggat ctacatgctg aatatttctc
    31081 agcgatggcg cttattgcta caagtattga acgttttgca acagagatac gcggattgca
    31141 aaaatccgaa acacgtgaag tagaagaatt ttttgcgaaa ggtcaaaaag gttcttctgc
    31201 tatgccgcac aaaaggaatc cgatcggttc agaaaatatg gctggtctag ccagagtggt
    31261 gcgcggacac atggtcactg cctatgaaaa tgtcacatta tggcatgaaa gagatatttc
    31321 tcattcttca gcagaacgaa tcatcttgcc tgataccacg attttattga attacatgtt
    31381 aaatcgtttt ggcaatatcg ttaaaaatct aacggtcttt cctgaaaaca tgaagcggaa
    31441 tatgtctgca acatttggtt tgatttatag ccagcgcgtt atgctgaaat tgatcgataa
    31501 gggaatgaca agagaagaag cttatgatct tgttcagcca aaaactgcgt acgcttggga
    31561 caaccaagtg cagttccgcc cgctgttaga agaagatcca accatcacat cggtattatc
    31621 taaagaagaa atagacgatg cctttgatta tcatcatcat ttgaaaaacg ttgacgtgat
    31681 attcaaacga gtaggtttgg aataaagaag agtgagttgc aaaattcaat taaaatagaa
    31741 tagcaaaaat aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt
    31801 atattttctt atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct
    31861 ctttacatac aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg
    31921 ctggttatgg agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt
    31981 gaataagatc tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat
    32041 tgaaagttcg cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca
    32101 gcgcttggtt gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa
    32161 ataaacgtct ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa
    32221 ttctgataaa aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga
    32281 acccattatc caaattttta gttaattcta atataccatc attaagtata aatgtaaacg
    32341 cttgttgatg gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg
    32401 ccttaaatgt acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa
    32461 aaaacgaaca aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg
    32521 gagaagaaag aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag
    32581 aacttgattt ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac
    32641 cagataagtg gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg
    32701 actcaaagag gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca
    32761 aaaaaagtac aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc
    32821 tcaaaacgtc taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac
    32881 tataaggatg accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa
    32941 cttgcaaaac cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt
    33001 ttgaccgaat tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc
    33061 cggtcgaatg gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg
    33121 gatgaacgta cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg
    33181 atcccagtct atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga
    33241 gtatgatgta tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag
    33301 gggaggcagt aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa
    33361 taggaaaata ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg
    33421 aaacgatttg tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca
    33481 aaaaaatgga ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt
    33541 gacgcagata tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg
    33601 catgatgagt cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat
    33661 ggtgactatt tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt
    33721 cgttttgctg aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca
    33781 gaagcaggac ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa
    33841 actgtaccat tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa
    33901 ataatctatc ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt
    33961 caagaactaa aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc
    34021 gtggaaaaca ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat
    34081 ccagaacgtg cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc
    34141 attttaaaaa acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct
    34201 gaagagatca agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga
    34261 atgatcgaag aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct
    34321 gttatgtgga gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct
    34381 actagtggac ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt
    34441 gatggactag cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct
    34501 tatgaaggag ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct
    34561 cgtccaattg cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa
    34621 tatttactag aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg
    34681 actgtaggcg gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca
    34741 atgtgcgtag gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt
    34801 aattcgatca tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt
    34861 gcctcagaag aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat
    34921 ccttttatgg aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat
    34981 tctctaatag gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg
    35041 gcttcaaaag ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact
    35101 gggatgacac cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt
    35161 aaatcaggag aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc
    35221 actatcggta aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta
    35281 gcaaatctgc cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa
    35341 gaaccagctc gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa
    35401 cctggacaag tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc
    35461 tacgaaactt atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct
    35521 gctgtgatga gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg
    35581 cgctatctgt atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga
    35641 aatattattg ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct
    35701 cctgataaac cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg
    35761 tgtgaaacgt taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat
    35821 ggtcaagcaa tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa
    35881 catatcacta ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg
    35941 tttgcagatt ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg
    36001 atccgaaact ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc
    36061 caaaacggat tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg
    36121 gcagaatcag ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct
    36181 caattatttg cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca
    36241 cgattcgaag aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg
    36301 ataatcgaaa tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag
    36361 tgctgggagg atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga
    36421 tcttcggtgt ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc
    36481 ttcagcatcg aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg
    36541 ggcatcgaga tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt
    36601 taacaggaga agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg
    36661 acaatattca gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg
    36721 gtaacttgat caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc
    36781 attctaactc cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt
    36841 ttttaaataa attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa
    36901 tgacagaaac ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg
    36961 gacaaatgaa aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg
    37021 gggcacgttt tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa
    37081 tccagattga ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct
    37141 attttgccag accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata
    37201 tgggacggaa tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta
    37261 attcttcttt gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg
    37321 gattagtaaa aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag
    37381 agcaaggtgt tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga
    37441 ttatggtcga tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga
    37501 aagaagcagg ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat
    37561 gtttttacgg gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg
    37621 atgaaatcaa agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga
    37681 tcgaatcgat cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca
    37741 acggagatta tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag
    37801 aaaaagtatc gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat
    37861 gcatacgcaa aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag
    37921 aagcatgtca aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt
    37981 tgttttgatt tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc
    38041 gtggggacga aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac
    38101 tgcgtagcga tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta
    38161 gactacttag caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta
    38221 gcagatggat gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct
    38281 ggtatgtact caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa
    38341 tctcaattat tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct
    38401 agcgggattc attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag
    38461 ttttctttat catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag
    38521 ccaacgaaaa tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga
    38581 gctgcacata tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct
    38641 atggctgcac aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa
    38701 aaatatggtg aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg
    38761 gtcctggcag tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc
    38821 gaaaaagttt atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata
    38881 ggagaagaac gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc
    38941 tagccgatta tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc
    39001 agcctgaagc atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt
    39061 cacctaaaga atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag
    39121 aaaaaaagat cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc
    39181 tgaaaaatta tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg
    39241 gtttgcatgg catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc
    39301 attatatcga ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg
    39361 aacaggagga cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct
    39421 atccagaagt gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt
    39481 gatcagtgta tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg
    39541 agtagaaatc atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac
    39601 gctttcgatc gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact
    39661 tcatcctaaa atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc
    39721 aatggaaacg gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa
    39781 agaaacgatt atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg
    39841 tggtcctagt atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga
    39901 tccatctgac tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc
    39961 tttcagacaa aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat
    40021 cgctcaatac ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta
    40081 tgaacgaaaa caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca
    40141 gtccgctctt ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact
    40201 ttcttataat aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga
    40261 gccaacggta gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat
    40321 tttagaagcg tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat
    40381 cgttttgaat cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga
    40441 aatcatcatt gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa
    40501 catccgatta ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt
    40561 ctcagtctta ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga
    40621 gtggaaagtc gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc
    40681 ttggagagct gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac
    40741 agtaggaatc ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca
    40801 agcgcaagag aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga
    40861 ttcggttgaa tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat
    40921 caaagaccaa gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga
    40981 tgttcggcat tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc
    41041 aggtggtcgt gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt
    41101 gtattgtgcg ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag
    41161 tgagcgagat cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat
    41221 cgttggtcct gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt
    41281 gaaaatcttc ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa
    41341 acaattaatg gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga
    41401 aaaagcagca gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt
    41461 agcagctggg aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga
    41521 acaaatgttg aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt
    41581 tttggcagga gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat
    41641 ggtcatttcg caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg
    41701 aatgggtgct tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga
    41761 agaagtttta aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt
    41821 tttatatgcg ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg
    41881 tttcggtgat cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt
    41941 aatcgatgat ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa
    42001 tgtaggcgtt gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct
    42061 accagatttt ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg
    42121 caaactagtt ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa
    42181 agaagcacag aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta
    42241 tcgtacagat attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg
    42301 cttccattta taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt
    42361 tcataaagag tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa
    42421 aaattcaatt aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta
    42481 tcaacaatgc ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt
    42541 tttttgacca tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc
    42601 tagcaaaaac atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc
    42661 caggtgtgat gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga
    42721 cccgtctgaa aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag
    42781 aaccagagga agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt
    42841 ttggcgtcag caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac
    42901 cattagtagc taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga
    42961 ttcatgtcaa tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt
    43021 cccgattgca tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag
    43081 gtgtatttat cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag
    43141 ctaaatatca aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca
    43201 atatgcaagt gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt
    43261 cagaaattaa tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt
    43321 taccttaaag attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact
    43381 tgaaagctgt tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat
    43441 cattgtacgg atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat
    43501 tcttttggag aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta
    43561 taaccaattt caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt
    43621 gctaattcga ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa
    43681 aaaatcgtac tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac
    43741 atatctggat cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga
    43801 tatttgttcc ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact
    43861 gtcgaacggt aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa
    43921 tgaaccattt cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc
    43981 aagacaaata aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt
    44041 tcgatgtact tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag
    44101 taaaagacag aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt
    44161 tgttctttaa gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt
    44221 aattcgctga ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc
    44281 ttttctaatt catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct
    44341 tcaagagaat caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact
    44401 tgttgattct ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag
    44461 tctgtactga cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga
    44521 ttgaaagatt cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg
    44581 ttcaagaaat gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt
    44641 tgctgcaaat ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta
    44701 atcggtttaa gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa
    44761 tcgtcgaatc cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt
    44821 gaaaaagtca acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt
    44881 aagtcgataa ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt
    44941 ggataataag aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg
    45001 tcttcgatga taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg
    45061 gatcatcagt gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg
    45121 atcatttccg taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact
    45181 gaaatctgaa tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg
    45241 gatgccaatc ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgcggt
    45301 aatacgaata aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat
    45361 caatggctgc aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat
    45421 cttatagtca agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg
    45481 gctaatttct ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa
    45541 gccggaaagt gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga
    45601 aataatggca tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg
    45661 aacttcactt tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag
    45721 atcttccgcc attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag
    45781 ggttaaacga aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt
    45841 gatcggttct tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat
    45901 ggtactaaga actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt
    45961 attttttttg cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag
    46021 cagttgagtg acagaatcta cctgattgat gatctcttca ttggtatcca taaaatcttt
    46081 tgccaagata ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag
    46141 cgatacatct aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat
    46201 atcattccta agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc
    46261 agaaatcaag tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc
    46321 tgctttttgg atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag
    46381 tatgctcagt agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa
    46441 aatctgtttt atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg
    46501 ggatgttagc tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta
    46561 atgtttttcc tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc
    46621 cgacatcagg tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca
    46681 tatcttcttg tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt
    46741 cttttatcgc agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga
    46801 tttcggataa atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa
    46861 taaaatcacc gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat
    46921 ctttttttag ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg
    46981 tcggtgtgga gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat
    47041 ttgtgaagtg attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc
    47101 caatgtgggt gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga
    47161 cagggatccc tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag
    47221 gagtaaaaat gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc
    47281 gttcctgatt catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag
    47341 tgataattga ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac
    47401 caacgactag tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag
    47461 cgattacaac aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga
    47521 tttgttagta ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa
    47581 aaaaatccaa gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag
    47641 taaatcccat aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa
    47701 ttgaaatggg ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat
    47761 tttgaccgca agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac
    47821 ggggtatgta ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa
    47881 caacatggtc tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga
    47941 taaagtagaa aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact
    48001 tgtgattgct tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc
    48061 agatatcaag gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta
    48121 cgcgactttt gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt
    48181 aggattgaaa gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga
    48241 caacaacgca ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa
    48301 cggtcagttg gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg
    48361 ggatggttct acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca
    48421 aacattggat gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt
    48481 aaaaggcgtg gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc
    48541 gacagttgct ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga
    48601 cacacgtatc ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga
    48661 ggttccggtc aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt
    48721 agcaaataca gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta
    48781 ttataaagaa tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa
    48841 gtcagagaga ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa
    48901 aaattttctg gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc
    48961 catgcattgt gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc
    49021 ttgtatccat atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt
    49081 aaagatttga aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta
    49141 agtccttatc tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc
    49201 gtgattgatt gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg
    49261 tctatcgacc cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa
    49321 atagctaaag cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca
    49381 ttgaatgaag aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt
    49441 gtaacctcca tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc
    49501 accattttac gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag
    49561 catattattc gcgggatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg
    49621 gatattggag aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac
    49681 gagcgaatca taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata
    49741 gctggactga tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat
    49801 ggaagcaaga tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt
    49861 ccttcagcca ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg
    49921 aatctgctga tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa
    49981 aaaggagtat tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg
    50041 cgtataaaaa caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa
    50101 aaagtcgttt tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca
    50161 acccgtggga tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca
    50221 gcaaatggca aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt
    50281 gatcgaatct atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca
    50341 gatcaggaag tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc
    50401 aaaaaatctt acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct
    50461 ttttggtcgc tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat
    50521 gacggatggt attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca
    50581 tatattagta ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc
    50641 ggtagggtca gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca
    50701 aatgaacccg tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg
    50761 gcaaggtttt tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt
    50821 gctgatgttt cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc
    50881 agctgttttc caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct
    50941 tcacttcttg acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg
    51001 gcgaacaaga aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt
    51061 ggtaaaggcg gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta
    51121 tcaagggtat ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt
    51181 gacaaataaa acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc
    51241 tgaactatca gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt
    51301 catggcagct ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc
    51361 aggtacatcg atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc
    51421 cggcggcatt ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa
    51481 caatggcatg tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat
    51541 cttgctttta gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa
    51601 cgccggttat tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa
    51661 aagcgaacat tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt
    51721 cgtataaatt ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc
    51781 gctaaaaaaa gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg
    51841 aggataatgt aatgaaagta tttgattacg aagatattca attaattcct aataaatgta
    51901 tcgtaaatag ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa
    51961 tgccagtagt gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag
    52021 cggaaaatgg ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta
    52081 tcaaaaaaat gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg
    52141 aatattcctt tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg
    52201 acatcgcgca tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt
    52261 tgcctgaaac ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg
    52321 aaaatgcggg cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa
    52381 aaatcaagac aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga
    52441 aagcagctcg caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa
    52501 aatctgtccg ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg
    52561 aatctcctgg cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg
    52621 cttcagaatt ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac
    52681 acaaaggatc tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt
    52741 cttatgctgg aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa
    52801 attcgatttt taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa
    52861 aagttgtcct gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg
    52921 tttggctgga attattatcg cagatacaag cttttgaaca tcattattca gaataaggga
    52981 ctgtgataag atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct
    53041 cgtaaattcg gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga
    53101 aaaagtagag aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga
    53161 aatcgaaaac ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca
    53221 actttttggt gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg
    53281 ggtactaacg agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt
    53341 cgctgttcca agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg
    53401 gtatgagcaa aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata
    53461 cgctagcgag atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga
    53521 agaaatctta gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa
    53581 aatcaaacgg tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt
    53641 gactggtggc ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga
    53701 ggggatagct gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg
    53761 aacgcgttta tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga
    53821 atcgatccat aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac
    53881 aggaaaagtg atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg
    53941 gcttccaatt attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt
    54001 ccttcctata cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt
    54061 tttaggaaaa acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact
    54121 agctcaagcg caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc
    54181 taatgtattt aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga
    54241 aaaaagccga ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat
    54301 tttaggtggt tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc
    54361 agaaaaagta gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat
    54421 aaatggaatc agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc
    54481 aaagatgaaa attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa
    54541 gaatataaca gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg
    54601 atataaagaa actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa
    54661 taaatgtttg caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta
    54721 agtttgatta gaaaatagaa aaaatgcaga tgaaaaataa aacgctttcc ttattgtgat
    54781 tttattcact aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt
    54841 gtgcttttag cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat
    54901 agtgatacag ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag
    54961 agaggaatgt gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag
    55021 atgaagtcaa tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta
    55081 acaaagattt agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg
    55141 tatggtctcg tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct
    55201 tttttgcacc aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata
    55261 aagaagatgt tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac
    55321 ctttatctca agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag
    55381 aattgaaagc tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg
    55441 ttgcattagg tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg
    55501 gcggttcatc acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa
    55561 atgccgtatt ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa
    55621 ctgcagcaaa aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg
    55681 atggaatgga tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca
    55741 gtggaaatgg accagtccta atcgaaacac tgacttatcg ttatggacca catacactat
    55801 ctggtgacga tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag
    55861 atccattgat ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag
    55921 aagaacaagt catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca
    55981 aagtaccaaa acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat
    56041 caatcaaaga acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa
    56101 aacaatgatc caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt
    56161 cgttgttttt ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt
    56221 gcaggaaaag ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc
    56281 aggattgagt ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt
    56341 tggttttatt ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg
    56401 aatgagcggc actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca
    56461 tacaccagaa ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg
    56521 agtagtgatt ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag
    56581 taacgatccg gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt
    56641 gccggatgaa gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga
    56701 tgtatctatt atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct
    56761 tgcaaaagaa aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt
    56821 agaaacgatc attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca
    56881 acgacaagcg ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc
    56941 attagaagcc ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc
    57001 agaaaatgtc tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga
    57061 attttaagaa aagaagggac gaaacaaagt catggcttat caatttaaat tgccggatat
    57121 cggtgaagga atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat
    57181 caatgaagat gatacattac tagaagttca aaatgacaaa tcagtagaag aaatcccgtc
    57241 acctgttaca ggaacggtta aaaatgtgat tgttccagaa ggtaccgtag caaatgttgg
    57301 ggatgtatta gtagaaatcg atgcaccagg acacgaagac aatgaagggg acagtggtgt
    57361 agccgcagaa tcccaaactc cagctaaacc ggctgctgaa cccactgttg atacagaatc
    57421 agctggatcg tcaagcgaag gagtcttcca atttaagtta ccggatatcg gtgaaggaat
    57481 cgcagaaggt gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga
    57541 tacattacta gaagttcaaa atgacaaatc agtagaagaa attccgtcac ctgtcacagg
    57601 gacagttaaa aacgtgattg ttccagaagg cactgtagca aatgttgggg atgtattagt
    57661 agaaatcgat gctcctggac acaatagtgc accgtcaact agtgcgccat cagctgaagc
    57721 accaaaagaa aaagtggaaa catcaggttc agcaagtgtc gttgaagctg ccgatccaaa
    57781 taaacgtgta ttggctatgc catctgttcg tcaatttgcc cgtgaaaaag atgtagatat
    57841 ctcacaagta actgcaactg gaaaaggtgg cagagtaaca aaagaggaca tcgaaaactt
    57901 cttggcaggt ggtccaagca gtgcaccagc gaaatcagaa gcgccagaag cggcagcacc
    57961 gaaagaagca gctcctgcag cagaaagcaa accagctgca ccagcaaaac cattcaagtc
    58021 caacttagga gacttggaag aacgtgtagc aatgacgcca acacgtaaag ctatcgctaa
    58081 agcaatggtc aacagtaagc atacagctcc acacgtgaca ttgcatgatg aagtagaagt
    58141 gtctaaacta tgggataatc ggaaacgatt taaagaagtt gctgctgcaa atggtacgaa
    58201 acttactttc ttgccatatg tcgtgaaagc tttaacagcg actgttaaga aatacccagt
    58261 attgaatgca tctatcgacg atgcaaacca agaaatcgtt tataaacatt actacaatat
    58321 cggtattgca actgatacgg atcacggact atatgtacca aatgtgaaag atgcagaccg
    58381 caaaggaatg tttgctattg cagacgaaat caatgagaaa gcaaaattag ctcatgacgg
    58441 caaactatct gcagaagata tgcgcaatgg aacaattacg atcagcaata tcggatctgt
    58501 aggtggcggt tggtttacac cagtcatcaa ctatccagaa gtagctattt taggtgtagg
    58561 aacgattgca caacaaccaa tcgtaaatgc tgaaggcgaa atcgttgtcg gtcgtgtgat
    58621 gaaactgtca ttaagtttcg atcaccgtat cgtagatgga gcaactgcgc aacaagcaat
    58681 gaataatatc aaacgtcttt tagctgatcc agagctatta atgatggaag gatgataaac
    58741 aaatggtagt tggagatttt gctgtagaat tagatacagt tgtaattggt gcgggacctg
    58801 gtggctatgt agctgccatc cgcgctgctg aaatgggaca aaaagtagca atcattgaaa
    58861 gagagtatat tggcggtgtt tgtttgaatg tcggctgtat tccttcaaaa gcactgatag
    58921 ctgcaggaca ccattatcaa gagtcactag attctgaaat gtttggagta actagtgaaa
    58981 acgtcaaact agattttgca aaaacacaag aatggaaaga aaacaaagtt gtccatacgt
    59041 tgacttctgg tgttggtttc ctattaaaaa aacataaggt tgaaacaatc gaaggagaag
    59101 cgttcttcgt agatgatcac acactacgcg tcattcatcc agattcagct caaacttatt
    59161 cgttcaataa cgcaatcatc gcaacaggtt cccgtccaat cgagattcca ggatttaaat
    59221 tcggaggacg tgtgttagat tctactggcg gattagcatt gaaagaagta cctaagaaat
    59281 ttgtgatcat cggtggcggt gtgattggtg ccgaattggg cggagcgtac gcaaacttag
    59341 gagcagaagt aacgattctt gaaggctcac cacaaatttt accaacttac gaaaaagatc
    59401 tagtaaaatt agtagaagat gatttcaaga aaaaaggcgt aacagtcgta acgaatgcaa
    59461 tggcgaaaga agcagtcgat aacggtgaca gcgtgacagt gaaatatgca gtggacggga
    59521 aagaagaatc cgttactgct gattatgtga tggtcactgt aggtcgtcgt ccgaatacag
    59581 atgatatggg cttagaacaa gcgggtgtcg aagtaggaga acgaggattg atcacggtgg
    59641 ataaacaagg acgtacgaac gtacctaaca tctatgccat cggtgatatc gttccagggg
    59701 cggcattagc acacaaagcc agttatgaag caaaaatcgc tgctgaagca atctctggca
    59761 aaaaagtagc tgtagattat aaagcaatgc cggcagtagc cttcactgat cctgaattgg
    59821 cttctgtagg tatgacgatc aaagaagcaa aagatgctgg tatcgaagca aaagcatata
    59881 aattcccatt ttcagggaac ggtcgggcat tatcgcttgg taaaacagaa ggattcatcc
    59941 gtctagttac gacgatcgaa gataacgtgc ttattggcgc gcaaatcggt ggagtcggcg
    60001 caagtgatat ggtctctgaa ttagctttag caatcgaatc aggaatgaat gctgaagata
    60061 tcgccttgac gattcatcct catccatctc ttggcgaaat cgtcatggat gcttcggaat
    60121 tagcattagg tttaccaatc catatttgat ttgaataata caggcgaaac gcaactgaaa
    60181 aatcagttgc gttttgtttt ttagaattat caataatatt attatgtaaa cttaaaaata
    60241 tgacaacttc ttggtttatt accacttcct atgctacact gaagaggaat atgaaatagg
    60301 agagttgaag atgcaagaat atcagtatcc acttgattta gattggacaa cagacgaaat
    60361 ggtcattgta atgaagatgt gggaagatct ggaacgtgcg aatgaaaaag ggataaacaa
    60421 tgaagctttt ttgaagactt atcagcagtt taaaactgtt gtcaaatcca ttggtgagga
    60481 aaaacgattg ggacgggaat ttgaaaaagc ttccggttat tctttatatc gtacagtgca
    60541 gcaagcaaaa aaaaatccaa acaaaacttt gagaatgaac gggtgagata aatggaacag
    60601 atgattgatc atatcaagtc ctggcttgaa gaagctggtg agaaaatcaa atcttctcaa
    60661 caaacattaa caatctcgca aaaatcaaat cgaacggatc tagtaacaaa tgtcgatcga
    60721 aacatccaga attttctaat cgaaaaaatc cattcctttg atccagaggc gaaaatattg
    60781 gcagaagaag aagggtgtaa tcaattatcg gatctcgatg gaagagtctt tatcatcgat
    60841 cctattgatg gcaccttgaa ttttgtgatt gaaggggaaa atttttgtat tatgctcgct
    60901 gtttatgaat ccggcgttgg aaagcttggt ttcatttatg acgtgatgag aaatgaactt
    60961 ttttggggtg gaaaaggaat aggcgtgtat aaaaatgatg agccattgcc tagaccagca
    61021 aataaatcct tatcggaagg cttacttggt gtgaatagtt atcttttcgg tcataataaa
    61081 tatggaatcc gagaaattgc ggaaagcagc atggggacac gtatgtacgg atgcgctggt
    61141 cttgaactag tggcaatctt gaaaggaaac cacattgctt atatttcaaa ccttagccct
    61201 tgggattatg cagcgggaaa tgtcttattg gaagagtttg gtatggtaac aagcggtctt
    61261 tttggtactc ctttgacatt tgaaaaaagg gaatactatt tagcagggac ttcttgtgca
    61321 tatgcagaaa tcgcagacct tttgaaaaaa gaaggctgaa aaaaaggtag aaatcattca
    61381 caaaaatgat aaaaacgagc aaaagattaa ttttctcgaa aaaatgaaaa cattttacta
    61441 tatttcatga aaaaagtctg ctataattag cagtcgagta aaatttgccc tgtcataaag
    61501 aaccaataaa aaaggagtta gttacattga attatagaaa cgatatccgt aacgtcgcaa
    61561 ttattgccca cgttgaccac ggaaaaacaa cattagttga tgaactatta aaacaatcag
    61621 atacattaga cgcacatacg caattgcaag aacgtgcgat ggactcaaat gcgctagaaa
    61681 aagaacgtgg tatcacgatt ttagccaaaa acacagctgt agactataaa ggtattcgcg
    61741 taaacatcat ggatactcca ggacacgcgg acttcggtgg cgaagtagaa cggatcatga
    61801 aaatggttga cggcgttgtg ttggtcgtag atgcgtatga aggaacaatg cctcaaacac
    61861 gttttgtatt gaaaaaagca ctggaacaac atatcacacc aatcgttgtc gtaaacaaga
    61921 ttgacaaacc ttctgctcgt cctgagcatg tcgtagatga agttcttgaa ctattcatcg
    61981 aattaggagc agatgatgat cagttagact ttcctgtgat ttatgcatct gcactaaatg
    62041 gtacgtcaag cttgtcagat gatccagcag atcaagaacc aaccatggca cctatttttg
    62101 atacaattat tgaaaaaatc cctgctcctg tcgataacag cgacgaacca ttacaattcc
    62161 aagtttcatt acttgactac aacgattatg taggtcgtat cggaatcgga cgtgtattta
    62221 gaggaacaat caaagtagga gaccaagtag cattaattaa attagacgga acagttaaga
    62281 aattccgtgt aacgaaatta tttggtttct ttggactaaa acgtctagag attcaagaag
    62341 caaaagctgg agacttgatt gctgtctcag gtatggaaga tatctttgtc ggtgaaacag
    62401 ttactcctgt ggatcatcaa gatgcgttac ctatccttca cattgacgaa ccaacattgc
    62461 aaatgacttt cttagttaat aattcaccat ttgccggtcg agaaggtaaa ttcgtaaccg
    62521 ctcgtaagat tgaagaacgt ttgatggcag aacttcaaac agatgtatcc ttacgagttg
    62581 aaccaacaaa ctctccagat gcatggacag tttctggtcg tggagaattg catttgtcta
    62641 tcttgattga aaacatgcgc cgtgagggat acgaattgca agtatctcgt ccagaagtta
    62701 ttgaaaaaga aatcgacggt gtaaaatgcg aaccgtttga acgcgtgcaa atcgatacac
    62761 cagaagaata tatgggtagt gttatcgagt ccttgagctt acgtaaaggt gaaatgcaag
    62821 atatggtcca tacaggaaac ggacaaatcc gtttgacatt cttaacacct gcccgcggtt
    62881 tgattggtta ttcaactgaa ttcctttcaa tgacacgtgg ttacggaatc atgaaccata
    62941 cttttgacca atatctgcca atgcttcctg gtcaaattgg cggacgtcac caaggggcgc
    63001 ttgtttcgat cgatacagga aaagcaacga cttattctat catgagtatc gaagaacgcg
    63061 gtacagtctt cgtagaacca ggtacagaag tatatgaagg aatgattatc ggggaaaaca
    63121 gccgtgataa tgatttgact gtcaacatca cgaaagcaaa acaaatgaca aacgtacggt
    63181 ctgctactaa agaccaaaca tctgtcatca aaaaaccaaa acaattgacg ttggaagaat
    63241 ctttggaatt cttgaatgat gatgaatatt gcgaagtaac accagaaagc attcgcttga
    63301 gaaaacaaat tttagagaaa aatgctcgtg aaaaagcatc taaaaagaaa aaataatctg
    63361 taaaaggatg aagagagaaa ccagcttgtt ataaaaaata gctggtttct ttttttgtcc
    63421 aactcttttc tgtggagaag tcatctttgt caatggacat aaattcagaa aatacgccga
    63481 aagacgttta aaagactttt aaaaaactta tttctagaga gtttttgttt cataataaga
    63541 taatgtttca cgaaaggaga tcaacttgtc cttcgaagaa aatcgaagta aaatggcaag
    63601 agaaaggagc gggaagatga acgacttatt aaaacaatac tttggttatg atgagtttag
    63661 acctggtcaa aaagaaatta tccagaaagt cattgatcag gaaaatgtat taggaatcat
    63721 gcctaccgga agtgggaaat caatctgtta ccagcttcca gctttactat tagatgggct
    63781 tactgtggtt gtgtcccctt taatctcttt gatgaaggat caggtggacg cggcgaatca
    63841 attaggtata cccgccactt ttatcaatag ctcgttagat ggttacgaaa cagctagacg
    63901 cttccaagaa atcgatagac agcagtaccg tttactttat atcgctcctg aacgttttat
    63961 catgccggat tttatccaag caatgaagcg atggaacgtc cgtatgatcg ctattgacga
    64021 ggcacattgt atctctcagt gggggcatga tttccgccca agttatttac agatggcgaa
    64081 ccagctagat caactgccaa atcgtcctgt aattgttgct ttgacagcta cagcgactgt
    64141 gcaagtagct gctgacataa aaagactttt aaaaattcca gaaaacaacc atattcagac
    64201 tggttttgaa cgagaaaatc tacgatttca agtaattaaa gatcaaaaaa aagaacaata
    64261 tctaatcgaa tatttaaaga tcaacaaaaa tcaatctggt atcatttatg cagcaacgag
    64321 aaaagaagta gaccgtctct atcatttgct taaaaaattc gatttttccg tgggtagata
    64381 tcatggtggt ctaaatgaga atgagcgaac agaaatgcaa gaagcctttt tatacgatcg
    64441 cttgcagctg attgtagcga caaatgcatt tggtatgggt ataaataaaa gcaatgtccg
    64501 ttttgttatc cattatcaaa ttcctggctc tctagaagca tactatcagg aagctggtcg
    64561 ggcgggaaga gatggtcttt ccagtgaagc catcctgctt ttctctcctc aagatatcca
    64621 agtccaaaag ttttttatcc agcagtccca aagagaagaa gggcaaaagc aaaaagaata
    64681 tgaaaaatta aaagcgatga cagaatacgt gtatattgag tcctgtcttc agcaatacat
    64741 cctaaactat tttggcgaga caagcagtcc gtgcaaccgt tgtggtaact gtttagatga
    64801 cagagaaata gtagaagtaa cgacagaagc acagatggtt ctttcttgct taaaacgtat
    64861 gggtgagaac tatggtaaac aaatgctgat gaaagtctta gcaggatcga aagaacaaaa
    64921 gctgcaagct ttaggttttg gacatttatc tacctatgga ctgatgaaaa accaatcgca
    64981 aaaagaaacg atgcagttaa ttgaatattt gatatcgaat ggctatcttc tgaccatcaa
    65041 tggtgagtat cctgttttga aagtaacaga aagagggata caagtattga aaggacaaga
    65101 atctgtttat cgaaaggaac ccaagaaagt ccagcagcta tcggatgaag aaaccgatac
    65161 actatttgaa gtgctgcggg aactgcgaac ggatctagca tcagaagcag gcgttccccc
    65221 gtatgtcgta ttttcagatt ccacattgaa agaaatgagc cgaatacgtc catcaagcag
    65281 attggagatg cttcagataa aaggagttgg ccaaagcaag cttgataaat atggcgaagc
    65341 ctttttatct cggataaaaa atgcggactc taacgttctg gataagtaga aagaacgaat
    65401 acgtaaaaat gaataaaaac aactggttaa atctgttttt tttgctcctt atgaaaatca
    65461 aatatgttat aattaaggaa ttagaatggg aggtaagcac agatggattc tttgtcgaaa
    65521 aaacttgcga tccaagtatt aaatgatgaa gcagaacgga tcaaacgatt aatccgaaat
    65581 caaaacaaca gtctctgtat ctcacaatgc aaagccttcg aagaagtggt agatacacag
    65641 atgtacggct tttcccggca agtcggtttt gctgtccgta taggagtaat cggaaagaac
    65701 gagggccagc tgatgttaag tgagctagaa cgagaattga atcagttata tagtgaggtt
    65761 taccaagaaa tttatgataa aaaagaagtt ggcaaggagg aatagacttg cctaagaaag
    65821 tgaaaaaaag gcatctatta gactatagca tcttgattcc ctatttaatt ttatgtgtat
    65881 tgggattgat catggtatat agttctacgt cgtatctatt attagaaaac ggacaaaatc
    65941 cgtccgcatc agtcattaac cagagtattt tttgggtgct tagcttgatt gctatcgcac
    66001 tactttataa aatgaaaaca gatgttttga aaaaccagcg tttgatcatg gcagccatcg
    66061 cagttttgac gattttgctg ctgattgttg tgttttttgg taaggagatc aatggggcga
    66121 aaggttggct gcaaattgca ggattctcga tccaacctgc cgaataccta aaaatcattt
    66181 ctatctggta cctgtctctg actttatcta aaagacaaaa cagtgtacaa aaagattttc
    66241 ttgggactgt gaaacgtcca cttgcaatgg ttatcggact tactgcatta gtcgctattc
    66301 ttcccgattt tggtaatgcg gcagttatct ttttgattat tttggttctg cttttagcca
    66361 gcggagtcaa ttatgtgtat actttgattg taggagtagg aggcttttgt ctaagtactt
    66421 ttacgatctg gctgatcaat atcacaaacg gaaagatctt acccggacgt ttacagtata
    66481 tctataatcg atttgctatt taccaaaatc ctttctcaga cgaattaaat aaagggcatc
    66541 aactagtcaa cggctactat gccatgttca acggaggact atttggcagg ggattaggta
    66601 acagtatcca gaaaaaagga tttttacaag aagcacaaac cgattttatt tatgcaatcg
    66661 tagtggaaga actgggagtg attatgggga ttttgatatt agcgttgcta ttctttatga
    66721 ttgtgcgcat tgtccttgta ggtatccgtt caaaagatcc gttcaattcg cttttgtgca
    66781 ttgggatcgg cgcaatgttt ttgatccaag tatttgtcaa tcttggagga attactggcg
    66841 ttattccttt gacagggatc acgttcccat ttttaagtca aggaggctcc agtttgctga
    66901 tgctttcgat ttgtgtcggc tttgtcctca acatcagtgc ggatgaaaaa cgcaagagct
    66961 taggtttata ataaaaaaag atgtaggagt ggttcgatga aaaaagtttt agttgctaac
    67021 aggggtgaaa tcgctgtacg tgtatttcgt gcttgtacag aattaggaat taaaactgtt
    67081 ggaatctatg cagaagaaga tgaatattcc gtacaccgtt tcaaagcgga tgaagcatat
    67141 ttagtaggac aaggaaaaaa accgattgat gcgtatctag atattgaagg gatcatttct
    67201 atcgcgaaag aatgcggagc tgacgctatt catccgggtt acggtctttt gtcggaaaac
    67261 ttgaattttg cgcaacggtg cgaagaagaa ggcatcattt ttgttggacc caaactgcat
    67321 catttagata tttttggaga taaaatcaaa gcaaaagctg cagctatcga agcggggatt
    67381 gcttcgattc caggcacaga tggaccaatc gcttctatag acgatgcctt ggagtttgcg
    67441 aaacaatacg gttatccgat tatgatcaag gcagctctag gcggcggcgg acgcggcatg
    67501 cgtgtagctc acgatgaaaa gagtgcaaga gagggctacg aacgtgcgaa aagcgaagca
    67561 aaagcagcct ttggtagtga tgaagtgtat gttgaaaaat acatcgctaa tcctaagcat
    67621 atcgaagtac agattctcgg ggatacgcat ggaaacgtca tccatctttt tgaaagagat
    67681 tgttctgttc agcgccgtca ccaaaaagta gtagaagtag cgccttgtgt ctctatgaat
    67741 gaacagcaac gtcaaaaaat ctgccaggca gctgtccaat tgatgaaata tgtcgggtat
    67801 gtcaacgcag gtacagtaga atttcttgtt gaaggagatg atttttactt tatcgaagtc
    67861 aaccctcgcg tccaagtgga acatacgatc acagaaatga ttacagatat tgatatcgta
    67921 acaacgcaac tgttgattgc tcaaggtttg gatctgcata aagaaatcgg attgcctcag
    67981 caagaaggaa ttaaattaaa tggttcagcc attcaatgcc gaatcactac agaagacccg
    68041 ttgaataact ttttaccaga cacagggaaa atcgatacgt atcgttcacc tggtggtttt
    68101 ggcgtacgtc tggatgtcgg aaatgcttac gcgggctatg tagtgacacc gtattttgat
    68161 tcattgctag taaaagtatg tacgcatggt gctacctttg aaacagccat tcagaaaatg
    68221 gaacgttgct tgaaagaatt tagaatccgt ggagtgaaaa caaacattcc atttatgctc
    68281 aatgtcatca cacatcctga attccaatca ggaaatgcaa aaacaacctt cattgatagt
    68341 acagcaacat tatttgaatt tcctcgacta cgagaccgcg gaaacaaaac gatgaaatat
    68401 atcggtgaaa ttactgtgaa tggttttcca ggtatcgaaa gtggcgaaaa accattttat
    68461 gaagaaccac gtatgcctaa agatctaatc acaagaactg attacgtaac ggctaagaat
    68521 gtattagatg cgaagggtgc cgatgctttg gtcgaatgga tcaaaggaca ggaaaatttg
    68581 ttgttgacgg atacgacatt tagagacgcc catcaaagtc tgctagcaac tcgtgttcgg
    68641 acaaaggatt tcaagcagat tgcacgtttg actggagaag gtcttcctga actcttttca
    68701 agtgagatgt gggggggagc aacatttgat gtggcttacc gctttttgaa tgaagatcct
    68761 tggcaacggt tgagaaaaat tcgttcttta atgcctaata cgttgctgca aatgcttttc
    68821 agaggttcaa atgccgttgg gtattctaat tatcctgaca atgtattggt ggaatttgtc
    68881 aaagaagcag cagcgcaagg aatcgatgta tttcgaatct ttgatagttt gaactggact
    68941 ccacaaatgg aaaaaagtat ccaagctgta cgggatactg gtaaaatcgc agaggcagct
    69001 atttgctata ctggcgatat caacgatcca agtcgggcaa aatacaatgt tcagtattat
    69061 aaagacatgg caaaggaact agaacaactg ggtgcgcata tcattgcaat caaagatatg
    69121 gctgggctgc ttaaaccgca ggcagcttat cgtttgatta gtgaattgaa agaaacgaca
    69181 gatctgccaa tccatctgca tactcatgat accagcggga atggaatcat cacttattct
    69241 gctgcttcaa aagcaggagt agacatcgtt gacgtggcaa tgagtgcaat gagtgggaat
    69301 actagtcagc ctagcatgag cagcttgtat tatgcactgg tcaatggtcc acggctgcca
    69361 gaaatcacga ttgaaaatgc acagaaatta aaccattatt gggaagatgt gcgcatgtat
    69421 tacaaaccgt ttgaaaacgg cttgaatgct cctgaaacag aagtatatat gcatgaaatg
    69481 ccaggaggac agtattcgaa tctacagcaa caggcaaaag ctgtcggatt aggtcatcgc
    69541 tgggatgaaa tcaaacaaat gtatcatact gtcaacttga tgtttggcga tatcgttaaa
    69601 gtaactccat catctaaagt agttggagat atggcactat ttatggtcca aaatgatctt
    69661 acagaagaag atatttatga aaaaggcgaa acactaagtt tccctgaatc tgttgtgact
    69721 ttcttccaag gagaactagg gcaacccgtt ggcggatttc ctaaaaaatt gcaaaagatt
    69781 attcttaaag gcagacctgc attgaatgaa cgtccaggtc ttttggctga atcagtcgac
    69841 ttcaacgaag taaaaaaaga attggcagaa aaaattggct atgaaccaaa acaagacgaa
    69901 gtattaagct atttgatgta tccgcaagtc tttttggact atcaaaaagc atataatcaa
    69961 tttggcgatg ttacattact ggacacacca acatttttcc aaggtatacg tttgggtgaa
    70021 acaatcaatg tacaaatcga acgcggaaaa acgcttatca ttcgtttaga tgaaatcgga
    70081 gaacccgata tcgaagggaa tcgcgtactg ttcttcaatt tgaatggaca aagacgtgaa
    70141 atcactatca aagataattc gatcatcagt acggtccaaa cccgattgaa agctgaaccg
    70201 acaaaccgag aacaaattgg agcaacgatg tctggttctg ttttggatgt attagtgaaa
    70261 aaaggcgata acgttaaaaa aggtgatacg ttgatgatta ctgaagcgat gaaaatggaa
    70321 acggccatcg aagcacgttt tgacggagaa gtcgcacatg tatatgtgtc ttcaggagac
    70381 actatttcgt ctggtgacct tttgattgaa gtgactgaga aataataagc gaaaggtggg
    70441 gaaacatgaa aagactgggt ttgttttggc tgattctgct attagttatt gtggcattct
    70501 acatggaacc agtcttgttt ccgccaaaac ttgaagtgaa ggaaaactct gatcaacaaa
    70561 tatcaaacca aatgacaacg tggaaatacc aggagcttaa agcaactggt ttttctactt
    70621 atatcggaaa accgatcgat cagctggaaa aggattttgg ccaagcctat gatcgattaa
    70681 ctagcggatt tggttttgaa acaagatatt atgaaaataa agctagtcat ctcacatttg
    70741 aagcgaacat ccaagacagc agagtcacaa ctgttaaagt actgcaaaca gggcctgctg
    70801 atatcgcgcc gttttattta gggatgacga tgcaagatct tacgaaaatc actactatct
    70861 atactaattt cacttttgag taccatgata cagaagttgg aatcgaacta atggaagagg
    70921 atatgaatta tcgtccgcta attgcttttg ataacgacac ttttgctatt ttattttttg
    70981 atcaaacaac aaacggccta ttttcagttg tgtatttaga taaagatagt cttttgaaac
    71041 tattgccata tcaagtgttt ggtgaaggcc ttccacatta tcaagcagat aaatcggctg
    71101 attgggaatc aatcaatcgc gttaaagaac gaaaaagtac gactttatta aatatgctta
    71161 gaagagaaga cgaacttccc atatacaatc aaacacttgc ctttcaaaac cacagtcagc
    71221 tgctactaca tgattttttg acaaggcctg aggaaatatt gtcagaggaa cgtatggctg
    71281 attggcaaaa atcgctaggc agtgctcaaa cgacagtgaa attctcactg acgaatgatg
    71341 aattggagaa gttaatcagt aaacaaaaat tagaaaatac tagtgggcta tttattcatc
    71401 cggcgattga cgctactttt tcgtttttat attggtacag cgatccttac aaccatgacc
    71461 gtttcatgga cccgggaacg gattctgtag gcattgcttt ttctaaagaa aatatgttag
    71521 tcttattgca agaagaaaac ggagaaagta gtgatagcag tgatcgttaa cgaagagctg
    71581 tttcaactgg aggatcaatg cctccaagtc gctgaaatga taaaaaagag taaaacgatg
    71641 gcttcttatc ttgaacataa acagtcaatg gaagaatctg caaaagtttg ccagttgaaa
    71701 gaagcgttct tgtctaagaa agaagcattt gaaaatgtag cggcatatgg tgagtatgca
    71761 cctgattatc gtgaaaaaaa acgagctgtt cgaaaagcaa aaagacagct tgatatggat
    71821 gagcatgttg cagctttccg atatagtgaa acacagctgc aaagtctgtt ggatcaaatt
    71881 gtccaagtac ttgccgaaga agtttctaat gaaatcaaag tagatgcagg tagcccgttt
    71941 tttgaaacga aaaaacactt tggatgcgga ggtaattgtc atgcaattag acaatagtga
    72001 atttgaaata acaaaacgac gcgggctgat cgtatgggtc tacagcttaa aacaattaaa
    72061 gaatctgaaa aagttcggct tgttgcatta tgtctcaaga agaatgaaat atgtcgtgct
    72121 ttacttaaat gaagagacat tggaagaaac agaagaacga atccgtaaat tacattttgt
    72181 tcgaaacgta gaacgttctt accgaccgga tatcgaaatg aattttgccg aaaaaatagg
    72241 aaacaaaaca gtattaaaag acgaaggttt cgaaattgaa gagctaaata cagaaatccg
    72301 attagctgaa catctttaaa aatctcttat gagcagtttt taaataaaaa ataacttgga
    72361 taacgatcga tttttgatca ttgtccaagt atttttttgg tatactagta ccagtaccat
    72421 ttctaaggag tgagtagatg cggataatat caggaaatta tggcgggcgc cgtttaaaaa
    72481 gtttagcagg ggcgaacacc agaccaacta cagataaagt aaaagaatcc atattcaata
    72541 tgattggacc atattttgat ggagagacag tattggatct gtttgcagga agcggtggtt
    72601 tagctatcga agcagtatct agaggctgca gtcatgcggt atgcgtagac aaaaattacc
    72661 aagcgctaaa aatcatcaaa gaaaacatcg aaattacgaa agaaccagag aaatttacca
    72721 caataaaact tgatgcggac aaagcaatcg aacggctagc agcggagaaa caacaattcg
    72781 actacttgtt tcttgatccg ccatatgcaa aacaaaaaat aatcgatcaa atccataaaa
    72841 tggaagaact gggacttttt tctaaaaccg cagttatcgt atgcgaaacg gataaaacag
    72901 tcgaattgcc agaaactgtg gctggattcc atcaaataag aaaacagatt tatggcataa
    72961 cggccgtaac tatctataga aaagagggaa atcaatgaaa cgtgcattat tcccaggaag
    73021 ttttgatcct tttacaaaag gtcacttaga tacggtggaa cgtgcggcta aattattcga
    73081 tgaggtagta attggtgttt ttatcaatac ttcaaagaaa agtttgtttc ccccagaaga
    73141 aagaatgaca ttgatcacaa aagctgtttc tcatcttcct aatgtaaaag tcatgcatca
    73201 agaaaatcaa ttgacggtag aaacagcaaa agagattggt gcagatgcgt tgatcagagg
    73261 catacgaagt atcaaagact ttgaatatga acgagagatt gctcagatga atcatcatct
    73321 atatgatgaa ttggaaactg tcttcttatt agcaaaacca gaatatagtc acgtgagttc
    73381 cagtatttta aaagaagtac tgcactttgg cggagatgtg agcagctact taccgccagt
    73441 gatcaacgaa gctttagctg gaaagcgtga atcaaatgaa agctaaacaa tggttgaaaa
    73501 cagtactgat tatcatattt ggaatgttga tcattggatt tgtagtagtt cccttgccgt
    73561 attatgtaga atcaccggga gccacgatcg atctgaaaga actaattacg gtcaatgata
    73621 aggaagataa aggttctggg tctttctctc ttacctctgt tggtatcagg caagctacag
    73681 gtttcttagc tttaaaagct aaaatgaccc cttttgaaga attgatcagt gaagaagaat
    73741 tgactggtgg ggcgacaaat gaagaatata atcaaatgca acagtattat atggattctt
    73801 cccaaaatgc agcaatcgaa caagcgttaa aacttgcaga caagccttat aaaatggaat
    73861 tcaaaggcgt atatgtcatg agtgtagaga aaacatcgaa tttctttggc aagctttcag
    73921 ttggagatac agtaacaaaa gtagacggga aaagctttca aagtacagaa gcatttatgg
    73981 attatgtaaa aagccaaaaa gttggacaaa caatcgaaat cagttatttg cataatggtg
    74041 aagaaaaaac agcttctggt gatctgatcg agttaccaac agataaaaag gcaggaatag
    74101 gtatcacgct tactgaccat accgagatcg aatcagatac agatgttcgt attaattctg
    74161 gctctatcgg tgggccatca gcgggattga tgttcacttt agaaatctat gaacaagtca
    74221 ctggtaaaaa cttgcgacat ggtaagcaaa ttgcaggaac tggaacgatc aacagtgaag
    74281 gagaagttgg ccgaatcgga ggaatcgata aaaaagtggc aagtgcggat aaagctggcg
    74341 tagagatttt ctttgcacca gatgatacga tttcagacga tgttaagaaa gaatatcctg
    74401 aaataaaaac caactacgaa gaagcaaaag ctgccgctaa aaaattgaat tcctcaatga
    74461 aaatcgtacc agtaaaaaca gtacaagaag cgcttgacta tctagagaaa atgaaatgaa
    74521 actaaataat taaagagggg ttgtgacacg agttctgtca caacccctct ttttcgtcaa
    74581 aaatcttcac gataaaaata aaaacaaaag aaaaaattga cgtaattaga tgttgaaggg
    74641 acgattttgc cagagctcac tgtttcttca attttaaatg aaaggagtaa tgaatgattt
    74701 ttactggtca ttctttgaaa aaagaacaat gatagaaaaa atcagcaaga aaatactagc
    74761 ttcacccaaa ttgatcattt ggatgtctat gatagttctt atcttttgta tcgcaagtgg
    74821 ttttttatta tcaaatttgc tattttatag agaaacagaa gagactgctg caaaagaaat
    74881 actttcttcg tttagttcca gtggagaaga tccatcagct gaaggaaacg atgtagaaga
    74941 ggatgctcgt ccaaaggtca tgtacaccga tatcaaagga agtgtgaagg agcctggtat
    75001 ctattccttt tcttcagaag aacgggtcta tgatgtacta aagcgtgcag gtggattatt
    75061 agaagaagcg gatagcgatc gaatcaactt ttctgcgaag atagaagatc agcaagtcct
    75121 atatatccct gctgtaggtg aagagctgcc agaacatctt gatcagttgg catcctcgga
    75181 aggaaaacag cctacagcag attccgagcc tcccaaaata aatatcaata cagccactcc
    75241 atcagaactt cagcagattc ctgggatcgg atcagtcaaa gcgcaagaaa tcattcgatt
    75301 tagagaagaa aacggatcct tccagaaagt ggaagacctg caggagattt ctggtatcgg
    75361 tgaaaagaca gttgaaaaac taaaaaactt cgttacaatt aaatagaatg attcaaaatc
    75421 gactggcacg agcaataaat ggacgctaat ggatactaag aataatccaa ttctaagagc
    75481 ttggcaggac gaggaggtag tatttttgga taggaaatag cttgattgtt cgcaaagaaa
    75541 cattaaaaaa accaatgctg aatgtctatc tccaaaataa aatatctggg attcatatta
    75601 tgaatacggc tgtcagcggc aataacagtc aagctctacg agaacgcttt gccaaagatg
    75661 tacttagcta tacagcagat aaagtattta ttctcattgg gacgaatgat ctagcagagc
    75721 ataaacaact ttctaaagaa acgtaccaaa aaatctgcag tggatgatag ttcagctgaa
    75781 aaaagactat agttctgatc atgctttctt tatcacgcca ccagcagtcg atgagacaaa
    75841 acaaaaatat cggaataaca agttgatcaa agaatatatc tctgttctca cagaagtagc
    75901 aaaacgccat gattgtgaag tatcgatttt tatacggaat ttcttgaaca aatggattcg
    75961 aagaattttt ataatgggct aatggatgac ggacttcatt tcggcagtga agggtaggat
    76021 cttttgtcag acaggatgat tcgttatatt taaagaatat tttgtttaag caatgagtac
    76081 tttttaatca acttgaaaaa acggaggaat ataaaacatg ttagaaaaat cttcaaaagc
    76141 atgcgatacg aataaagaaa aagttactga taagtcacat aatgaacgca ttccttggga
    76201 ccagtacttt atggctcagg cagtactgtt atctttacgt agtacgtgta cacgtttaga
    76261 agtaggagcg acactcgttc gagaaaaaag gatcattgct ggcggctata atggagcagt
    76321 cagtggagat gtccactgca tagatgaagg gtgttacgtc gtggatggac actgcttgcg
    76381 aacgatccat gcagagatga atgcactcct acagtgtgca aaacttggta tcccgacgga
    76441 aggttcagaa atctatgtca cacattttcc atgtttggcc tgtacaaagg ctttgttgca
    76501 agctggtgtg aaagggatca attatctcca tgactatcga aatgatcctt atgcccaagc
    76561 gttgatcgaa caattaggtg tatctgtaca ccaagttgct ttagactcac agcatttttc
    76621 aaaacttcaa aatgaattaa caggaaaaca tgtctagcgt tttagaaagg catgttcgaa
    76681 atcggttgat ttttccggca attacgtcga tgattatctg tttgttgtat tacactgaca
    76741 attggggtta tcgtttgctt cttgccggaa tttttttatg gttttcgcta aaagaaggac
    76801 gttttatcgc gtacttgtgt attttgtgta gcataacggt atccatttct tataggctac
    76861 aagagtattc atttttagag gaaaaaacaa catctgcgca aattactgta tttacagata
    76921 cgattcgtgc aaatggtaat tttttgacaa tggaaggaag gttatcagag aataaacaaa
    76981 agctccagct ttcttatcaa gtaaacacag aagcagaatt gaaaacatgg cttaacttcc
    77041 gaggaaaggg aatagaattg actgctgaag gcgagttcat ctctccagca aaaaagcgga
    77101 atctttataa ttttgaccaa gaagcttact tcaggaacca tagaatttct ggacggttcc
    77161 aaattcaaaa gattgaacag atcaccatgc aaaatcactg gcgaaactgg cttcagagaa
    77221 aaagaggatc ttttatctcc tttgtccaat ctgaatttcc gcaaaaaaca agtgtatata
    77281 ttaattcttt attatggggc tataaagatg caggattcag agaaccagaa gaaattttta
    77341 gggagagtgg tttgctgcat ctgttcagtc tatcgggtct acacatccaa ttttatttag
    77401 ggtggctgta ttatttattt cgacgtatcg gatggccact acacgtcagt attattccgt
    77461 tgagtctact aactatctgt tatgtttcta ttgcaggaag ttctatttct gttttgcgtg
    77521 ctagtttgct gtttttattg cgattgctag tcaaattgct gaataagcat tattccacca
    77581 tggacttatt tgcagttgtc ttttggcttc ttttactatt tgatcctcga tttctttttc
    77641 atgcgggtgg acagcttagt tttttcatga gttttttgtt tatcctgatt tcttttgatg
    77701 gagattggtt gaaaaaaacg gcctctcacg tcctgtttac tatgatcacg atgccgttga
    77761 ttgtatggca tttttatgag tggcctattt taggtagtct ttttacatta ctgattgcac
    77821 cggtattcaa gtttcttttt cttcctggtg tgttatttct cacactcttc aatcgctggc
    77881 tgccggaaac tttacttttg ctgatagaag agggattaat cttatttgag cgaataatcg
    77941 atttttcttc agggtcgtct ctcattatag gacgtttgcc ccttgtgcta tctgggatgt
    78001 atattttcgg attactgatt tgtatggata ggttgaaaga acaacctgtg aaacacttgt
    78061 tttatgttgg tcttttttct tttatttttt tgactttgcc atttttacgc ttttcttcaa
    78121 cgatcgcttt tatcgatgtc ggtcaaggag acagtatctt tttgcaaagt ccttttcgaa
    78181 aagaaacgat tttgatcgat actggaggtc agttacactt tgaaagagaa aagtgggcgc
    78241 aaggtatgat acgtcctcct gctgaaagaa atatcgttcc atatctaaaa ggtcagggga
    78301 taagtgtaat cgataaactc atcttgacac acgacgatac ggatcatgtg ggagaattac
    78361 ccacgttatc tcagcatttt actatcaaaa aaatttatat cggttggggc gctggacgaa
    78421 atgagccatt acaaaggcat ttatctgaag ctcaaaaaaa tggtactgag atcattgaaa
    78481 tcaaacatgg ggacaggatc agcggttatt atgatcttta tgtattgact ccttttgaaa
    78541 agggagaggg aagaaatgaa gattcgattg gtctatggat ggaatataac aataggcgct
    78601 ttttattttt aggagatttg aatcaagcaa tggaaaaaca attgcttttg atttatccta
    78661 atctgaaagc cgatgtagtc aaattaggtc atcacggtag ccgtacgtca agtaacacag
    78721 attttctttc ccatattgaa gcaaaacatg ggattatatc ctgcggcgta aataatcgtt
    78781 atggtcaccc gcacagcgaa gtattggaga cactagaaga aaatcagata aaaacatggc
    78841 gaacggatca acaaggaatg atcacctatc gctggcatcc agtttttcat cctcatgggc
    78901 tagtggaaac aatgatagac tagtaattct catctgctca tggtaggata atttttgaaa
    78961 gtggtgagta atttgagtat acaagcagag ttacaaaaaa tacgtaaaga aaaacttgca
    79021 tcttgttact tgattttagg aacagaaaag tttctccaag atcaagttcg gacagaaatt
    79081 gtcaaaaaaa tccaattaga gggcgaagat gacttaaatt ttttaagttt tgacatggaa
    79141 aatgcaagtt tagatgaggt agtagctgaa gcagagacgt tgccgttttt cggagatcaa
    79201 cggttagtat tcgtagagaa tccttatttt ttaacaggtg aaaaagtaaa caatggaatc
    79261 gaacaaaata ctgatttatt gacggcttat ttgaaagaac cattagaatc gactgtatta
    79321 gtattctttg cgccatatga aaagttggat gaacggaaaa aagtaacgaa acaattgaaa
    79381 aaaacagcaa ttatcatcaa tgtccaacag ttgaatgaaa aagaagtacg tcaatatctg
    79441 atgaatacct tagagaattc ctcaatcaaa atggaacgaa gtgcaattga tttattcttg
    79501 aggttgaccg acttagattt atccaaaatg atgagagagt tacagaaatt ggtcctttat
    79561 ggacaaaatc aacagcaaat cacggtaaga gaagtagaag aattagttcc taaaacgtta
    79621 gaacataata tttttgacat gacccaatat attttaaaag ggaagacgga acaagctttg
    79681 cgtttatacg aagatttagt tatgcaaggg gaagaaacaa tcaaaattaa tgcgattttg
    79741 ctttctcaat tacgtttatt tttacagaca aaatttcttg taaagattgg ttatcagcag
    79801 gcaaatattg ctgaaacatt gaaaatccat ccatatcgtg taaaacttgc catgcaggaa
    79861 gtaaaaaaat tcgatgaaca tttgctggta cagttgtttg atcaattagt tgagatggat
    79921 tatcaaatca aaacgggaca aatcgaaaaa gaattaagtt ttcaactatt cgttttgcgc
    79981 acagggcagt tgttaagaaa tcgataagaa gaaagacgag ggcaaagcat gcgggaaggt
    80041 cgtttgtttt tgtccttttt ttgacaaata aaaaaatcgg ccgaagccga ttcttttatt
    80101 tagcgatttt tttgcttaaa cgagatttgt cgcggttagc tttgtttttg tggattagac
    80161 cttttgattc agccatatca atagctttaa cagcttcttt atataacgca tctacgttgt
    80221 cagcacctga agctacagca tcttcaaatt ttttgattgc agtacgcata gcgcttgttt
    80281 gagatgaatt tttcacatta gcgttttcgc ttgtacgaac acgtttgatt gcagattcaa
    80341 tgtttggcat tagtttcacc tccgataact taagttctta cgcgtgttac cgcataattc
    80401 aacattcatt attataccta aaggcagcgt gctttgcaat atgaacatga caagtttttt
    80461 tccttaacag tcttttatta ggattttttt tcagtaaaaa cggatagtac aagctgaaaa
    80521 aaatcaaaaa gacgctattg tgggaggcaa tagcgtcaaa ccaaactaga tttttattta
    80581 tctatttaaa caaagtttga atgggaatgt ttggtactaa gttattatat aacaaagaga
    80641 accgtttgca aagttttttt tgaaaaggga tacgattccc tgttgttctt tgtttttcta
    80701 ttaagaaact gcagttattt tataaccagt atcctttgat cagtaaaggc tattctggtg
    80761 taccgattat tttaatgtat tcatcgatat tttttatcaa catttttttt atgaccaact
    80821 ggttccgacg gttccatttg ccacttactt ccagcacata tttatttagg ggcaaacgta
    80881 agatatattt tgccaaatag cgatctttaa gcaggccatt gatcacacgg tcctcaagct
    80941 gcaagttgat cccagtcagc atcattggct gttcttgtaa aacgtacgta tcaattaaaa
    81001 gaccttcaaa agtttccaca agcgtcttcc tttcttttca aagtaacctg aaaacaggca
    81061 ctaattcttc cattttgata ttcgttgaag tggatgcgat cattctttct tttgtccact
    81121 gtaatctagt ataacaaaaa agagcagaag agccaaaaag aaaaaagagc atcgaattct
    81181 ataggaagtc tcattggcac ggcctaaatt attacaaaac attgattaaa aaaagcccct
    81241 gcatcgcagg agcatcccct atatcgtttt aataggtcaa tcaaaaagca gtatcaaaca
    81301 cattttgtat caaatcagga aataatagat aagctaagcc cagaatcaaa agtaccgcta
    81361 gaagaaacca gccgattgtt cctatgaaat actttaattt ttcgaacatt gtatcacctc
    81421 gttgtcagta tatcacggat cagtaaattg ggggtgttaa gaatctccta aaatagacaa
    81481 aatttgttct attttcctaa agcaaatact tgtaagatac agtaagtgat accagaagca
    81541 atgaccggtc cggcggcgat tcctcttaag aagacaacac ctaaaatcgt gccgaaaaca
    81601 agtgcaacag tgatttgtgg atcagaagca atcaatccta cacccttgga agataagaca
    81661 gccactagcc caccgcaaaa aacagctacc cagccaatag gggatttaaa ggcttctaat
    81721 agatcacgta gaccgatttc tcctgttgcg atagggatta gaatagccgc tgtgatgatc
    81781 gtcaccccaa tattgattcc tttgccttga agccaaggga aaaaggaacg ggaggctggt
    81841 atgagcttca taatcaaaac aaaagctgcc gctatcatca gactttgatt tttagctaaa
    81901 attgctacaa gtaaaataat tcctaaaaaa agccaacttt ccatgaaata tattcctcac
    81961 tttcttcgaa acaatcataa catgttttta gaaagttaca aaagtaggaa ttcatccttc
    82021 aagacctatt cataagctgt ttttttcata aaataaacaa aatcataaaa aaacactttg
    82081 cttatttgac agaagacagc cgcgctttgt atagtggaca agacttacat ttttttgaag
    82141 aatcagaaag atcaggtaaa ggtcttagta aacgcaagcc attcagaata accaatattg
    82201 tgcttccttc gtgtcctaca actccaagag gtagatttaa aaattgcagg aagttagaag
    82261 caatcaacaa gagaataaca agaatcgaaa aaataatatt ttgtttgatg atccgccgca
    82321 tttttaaaga tagccgatga ctgtagacaa atttatttaa gtcgcttttc attaagacga
    82381 tgtctgcgat atccatagcg gcatctgtcc cttgccccat agcgacacct aaagaagcgg
    82441 ctgctaaagc aggcgcatca ttgactccat caccaaccat cgcatttact tggtaaagtt
    82501 cattctcttt tttgactagt tgtgtttttt cttccggagt acagcttgca tagtaatgtt
    82561 caatccctac ttgttcagca atggcctttg ctgtgccaga atggtcgccg gttatcatac
    82621 ttgtatggat gttttgggat ttaaagtagg aaattacttg ttgggtattt gcttttggaa
    82681 tatctagcag acctaaaaca gcaattaact gctgatcttt agaaagataa atcacggttt
    82741 tgccttgatt ttccaaacgt tcgatcattt ccgcaatctc tttgctgatt atcatcgtct
    82801 caggatcata tgcgtgcttg ccaactttcc attgcgcacc taaataggta gtttgtaatc
    82861 caaaaccagt aatttcttct attggtaagt attggatttc ttcaggtatt gtgcaatcga
    82921 aatattgcaa aatcgcatga gcaagtggat gcgtcgtttt ttgttccata gcaacaagaa
    82981 gttgttgtgc caaatccttt tcatcaaaga aaaagctgtc agttacaact ggttttcctt
    83041 tagtgagcgt tcctgtttta tcaaaggcaa tcgcttttaa ttcagaaagc ttttctaaat
    83101 ggataccgcc tttgattaaa atcccatttt ttgcagaatt tgaaagagca gctagagaag
    83161 ctggtgtagc agatgcgaca agcgcacaag gagaagccac aactaataat accattcctc
    83221 gataaaagct ttcatcccaa ctccaaccta ggaaaaagtg ggggagaagg atcattagag
    83281 gaacagcaag caaaatcaat tttacataat tattttcgat tcgttcgata aatgaagctg
    83341 tttgagttgg cgtattctgc gcttcttcta ctaattggat aatcttagca aagacagttt
    83401 cggaactcgt ttttgttact cgaatcgtga tgggatttcc ttgattcagc gttcctgcat
    83461 acatctcatc gcctaaatat ttttctacag gcacagattc accgcttaat gcagactcat
    83521 cgatcgttgc ctgtgtgctt tctaaaaagc catctatcgg aacagctgct cctttagcga
    83581 caaataccaa gttatccacc tttagttgag aaacatctac ttcttgcatt tgtccatttt
    83641 cttttaataa ttgggctttt tgtggttgaa gattcatcaa agcagtaatt tctttttgac
    83701 ttttattagt tgtgtattcc tctaatgcac cgcttaaaca aaagatgaat gtcaacattg
    83761 ctccttcaaa gtaattaccg atcaaacagg caccaatagc agctaaagcc atcaataaat
    83821 ccacattcag tgtccgatca ttccacaatt ctttcaagcc ttccgatgtt tgtttatatc
    83881 caccgatgat aatggcactg atataaaaaa gtggggtgat ggtaagagag aatcggtcga
    83941 agataaaacc gaagatcata aataaaaggc agccgattgt tgctgcaaaa gcttttttct
    84001 gattagtcat cgttattaga cctccttgtc aaaacaagga tagcataata aaaatgtaat
    84061 tgataataat aacagctaaa tgagaacgat aatggttatc atttggtgat ta
//
LOCUS       DARBFD010000004        83042 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000004 DARBFD010000000
VERSION     DARBFD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.004"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            52..570
                     /locus_tag="SCK08_000235"
     CDS             52..570
                     /locus_tag="SCK08_000235"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1200155.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            698..1381
                     /locus_tag="SCK08_000236"
     CDS             698..1381
                     /locus_tag="SCK08_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEG1200156.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1690..1763
                     /locus_tag="SCK08_000237"
     tRNA            1690..1763
                     /locus_tag="SCK08_000237"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1724..1726,aa:Arg,seq:tct)
     gene            1922..2281
                     /locus_tag="SCK08_000238"
     CDS             1922..2281
                     /locus_tag="SCK08_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEG1200157.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2404..4035
                     /locus_tag="SCK08_000239"
     CDS             2404..4035
                     /locus_tag="SCK08_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEG1200158.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4097..4738)
                     /locus_tag="SCK08_000240"
     CDS             complement(4097..4738)
                     /locus_tag="SCK08_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1200159.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4924..5000
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5020..5328
                     /gene="rplU"
                     /locus_tag="SCK08_000241"
     CDS             5020..5328
                     /gene="rplU"
                     /locus_tag="SCK08_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEG1200160.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5355..5699
                     /locus_tag="SCK08_000242"
     CDS             5355..5699
                     /locus_tag="SCK08_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEG1200161.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5712..6005
                     /gene="rpmA"
                     /locus_tag="SCK08_000243"
     CDS             5712..6005
                     /gene="rpmA"
                     /locus_tag="SCK08_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEG1200162.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6439..9246
                     /locus_tag="SCK08_000244"
     CDS             6439..9246
                     /locus_tag="SCK08_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1200163.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9437..9928
                     /locus_tag="SCK08_000245"
     CDS             9437..9928
                     /locus_tag="SCK08_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1200164.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10031..11020
                     /gene="manX"
                     /locus_tag="SCK08_000246"
     CDS             10031..11020
                     /gene="manX"
                     /locus_tag="SCK08_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEG1200165.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11053..11856
                     /locus_tag="SCK08_000247"
     CDS             11053..11856
                     /locus_tag="SCK08_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEG1200166.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11877..12791
                     /locus_tag="SCK08_000248"
     CDS             11877..12791
                     /locus_tag="SCK08_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEG1200167.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12874..13248
                     /locus_tag="SCK08_000249"
     CDS             12874..13248
                     /locus_tag="SCK08_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEG1200168.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13387..13632
                     /locus_tag="SCK08_000250"
     CDS             13387..13632
                     /locus_tag="SCK08_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1200169.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13804..14868
                     /locus_tag="SCK08_000251"
     CDS             13804..14868
                     /locus_tag="SCK08_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1200170.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15114..15539
                     /locus_tag="SCK08_000252"
     CDS             15114..15539
                     /locus_tag="SCK08_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1200171.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15532..15981
                     /gene="nusB"
                     /locus_tag="SCK08_000253"
     CDS             15532..15981
                     /gene="nusB"
                     /locus_tag="SCK08_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEG1200172.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16078..16932
                     /locus_tag="SCK08_000254"
     CDS             16078..16932
                     /locus_tag="SCK08_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEG1200173.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16933..18270
                     /locus_tag="SCK08_000255"
     CDS             16933..18270
                     /locus_tag="SCK08_000255"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEG1200174.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18275..18499
                     /locus_tag="SCK08_000256"
     CDS             18275..18499
                     /locus_tag="SCK08_000256"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEG1200175.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18501..19391
                     /locus_tag="SCK08_000257"
     CDS             18501..19391
                     /locus_tag="SCK08_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1200176.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19400..20218
                     /locus_tag="SCK08_000258"
     CDS             19400..20218
                     /locus_tag="SCK08_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEG1200177.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20325..20774
                     /locus_tag="SCK08_000259"
     CDS             20325..20774
                     /locus_tag="SCK08_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1200178.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20792..22474
                     /gene="recN"
                     /locus_tag="SCK08_000260"
     CDS             20792..22474
                     /gene="recN"
                     /locus_tag="SCK08_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEG1200179.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22529..22645)
                     /locus_tag="SCK08_000261"
     CDS             complement(22529..22645)
                     /locus_tag="SCK08_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEG1200180.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22718..23674)
                     /locus_tag="SCK08_000262"
     CDS             complement(22718..23674)
                     /locus_tag="SCK08_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1200181.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23845..24213
                     /locus_tag="SCK08_000263"
     CDS             23845..24213
                     /locus_tag="SCK08_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEG1200182.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24383..24814
                     /gene="mraZ"
                     /locus_tag="SCK08_000264"
     CDS             24383..24814
                     /gene="mraZ"
                     /locus_tag="SCK08_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEG1200183.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24835..25794
                     /gene="rsmH"
                     /locus_tag="SCK08_000265"
     CDS             24835..25794
                     /gene="rsmH"
                     /locus_tag="SCK08_000265"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEG1200184.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25799..26200
                     /gene="ftsL"
                     /locus_tag="SCK08_000266"
     CDS             25799..26200
                     /gene="ftsL"
                     /locus_tag="SCK08_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEG1200185.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26201..28393
                     /locus_tag="SCK08_000267"
     CDS             26201..28393
                     /locus_tag="SCK08_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1200186.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28419..29381
                     /locus_tag="SCK08_000268"
     CDS             28419..29381
                     /locus_tag="SCK08_000268"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEG1200187.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29395..30756
                     /locus_tag="SCK08_000269"
     CDS             29395..30756
                     /locus_tag="SCK08_000269"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEG1200188.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30782..31870
                     /gene="murG"
                     /locus_tag="SCK08_000270"
     CDS             30782..31870
                     /gene="murG"
                     /locus_tag="SCK08_000270"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEG1200189.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31885..33105
                     /locus_tag="SCK08_000271"
     CDS             31885..33105
                     /locus_tag="SCK08_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEG1200190.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33261..34586
                     /gene="ftsA"
                     /locus_tag="SCK08_000272"
     CDS             33261..34586
                     /gene="ftsA"
                     /locus_tag="SCK08_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEG1200191.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34609..35850
                     /gene="ftsZ"
                     /locus_tag="SCK08_000273"
     CDS             34609..35850
                     /gene="ftsZ"
                     /locus_tag="SCK08_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEG1200192.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35867..36544
                     /locus_tag="SCK08_000274"
     CDS             35867..36544
                     /locus_tag="SCK08_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEG1200193.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36558..37157
                     /locus_tag="SCK08_000275"
     CDS             36558..37157
                     /locus_tag="SCK08_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEG1200194.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37172..37459
                     /locus_tag="SCK08_000276"
     CDS             37172..37459
                     /locus_tag="SCK08_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEG1200195.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37508..38290
                     /locus_tag="SCK08_000277"
     CDS             37508..38290
                     /locus_tag="SCK08_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEG1200196.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38311..39018
                     /locus_tag="SCK08_000278"
     CDS             38311..39018
                     /locus_tag="SCK08_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEG1200197.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39280..42078
                     /gene="ileS"
                     /locus_tag="SCK08_000279"
     CDS             39280..42078
                     /gene="ileS"
                     /locus_tag="SCK08_000279"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEG1200198.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42127..43653)
                     /locus_tag="SCK08_000280"
     CDS             complement(42127..43653)
                     /locus_tag="SCK08_000280"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEG1200199.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43668..44315)
                     /locus_tag="SCK08_000281"
     CDS             complement(43668..44315)
                     /locus_tag="SCK08_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEG1200200.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44499..44828)
                     /locus_tag="SCK08_000282"
     CDS             complement(44499..44828)
                     /locus_tag="SCK08_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEG1200201.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45005..45733
                     /gene="sfsA"
                     /locus_tag="SCK08_000283"
     CDS             45005..45733
                     /gene="sfsA"
                     /locus_tag="SCK08_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEG1200202.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45749..46762
                     /locus_tag="SCK08_000284"
     CDS             45749..46762
                     /locus_tag="SCK08_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1200203.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46762..48039
                     /locus_tag="SCK08_000285"
     CDS             46762..48039
                     /locus_tag="SCK08_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1200204.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48102..50804
                     /locus_tag="SCK08_000286"
     CDS             48102..50804
                     /locus_tag="SCK08_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1200205.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50956..51273
                     /locus_tag="SCK08_000287"
     CDS             50956..51273
                     /locus_tag="SCK08_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1200206.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51303..51623
                     /locus_tag="SCK08_000288"
     CDS             51303..51623
                     /locus_tag="SCK08_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1200207.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51731..53191
                     /locus_tag="SCK08_000289"
     CDS             51731..53191
                     /locus_tag="SCK08_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1200208.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53259..53480
                     /locus_tag="SCK08_000290"
     CDS             53259..53480
                     /locus_tag="SCK08_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200209.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53511..53693
                     /locus_tag="SCK08_000291"
     CDS             53511..53693
                     /locus_tag="SCK08_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEG1200210.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53693..54106
                     /locus_tag="SCK08_000292"
     CDS             53693..54106
                     /locus_tag="SCK08_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEG1200211.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54229..55410
                     /locus_tag="SCK08_000293"
     CDS             54229..55410
                     /locus_tag="SCK08_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1200212.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55481..55645)
                     /locus_tag="SCK08_000294"
     CDS             complement(55481..55645)
                     /locus_tag="SCK08_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200213.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55799..55872
                     /locus_tag="SCK08_000295"
     tRNA            55799..55872
                     /locus_tag="SCK08_000295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55833..55835,aa:Arg,seq:cct)
     gene            complement(55909..56109)
                     /locus_tag="SCK08_000296"
     CDS             complement(55909..56109)
                     /locus_tag="SCK08_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1200214.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56414..57646)
                     /locus_tag="SCK08_000297"
     CDS             complement(56414..57646)
                     /locus_tag="SCK08_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEG1200215.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57900..58469
                     /locus_tag="SCK08_000298"
     CDS             57900..58469
                     /locus_tag="SCK08_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1200216.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58647..59087)
                     /locus_tag="SCK08_000299"
     CDS             complement(58647..59087)
                     /locus_tag="SCK08_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEG1200217.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59245..60009
                     /gene="map"
                     /locus_tag="SCK08_000300"
     CDS             59245..60009
                     /gene="map"
                     /locus_tag="SCK08_000300"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEG1200218.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60041..60964
                     /locus_tag="SCK08_000301"
     CDS             60041..60964
                     /locus_tag="SCK08_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEG1200219.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            61040..62179
                     /locus_tag="SCK08_000302"
     CDS             61040..62179
                     /locus_tag="SCK08_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEG1200220.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62172..62972
                     /locus_tag="SCK08_000303"
     CDS             62172..62972
                     /locus_tag="SCK08_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1200221.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62972..63799
                     /locus_tag="SCK08_000304"
     CDS             62972..63799
                     /locus_tag="SCK08_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1200222.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63777..64511
                     /locus_tag="SCK08_000305"
     CDS             63777..64511
                     /locus_tag="SCK08_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1200223.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64611..65477
                     /gene="rfbA"
                     /locus_tag="SCK08_000306"
     CDS             64611..65477
                     /gene="rfbA"
                     /locus_tag="SCK08_000306"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEG1200224.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65491..66063
                     /gene="rfbC"
                     /locus_tag="SCK08_000307"
     CDS             65491..66063
                     /gene="rfbC"
                     /locus_tag="SCK08_000307"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEG1200225.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66085..67113
                     /gene="rfbB"
                     /locus_tag="SCK08_000308"
     CDS             66085..67113
                     /gene="rfbB"
                     /locus_tag="SCK08_000308"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEG1200226.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67211..68062
                     /gene="rfbD"
                     /locus_tag="SCK08_000309"
     CDS             67211..68062
                     /gene="rfbD"
                     /locus_tag="SCK08_000309"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEG1200227.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68097..70130
                     /locus_tag="SCK08_000310"
     CDS             68097..70130
                     /locus_tag="SCK08_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200228.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70174..71454
                     /locus_tag="SCK08_000311"
     CDS             70174..71454
                     /locus_tag="SCK08_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEG1200229.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71664..72470
                     /locus_tag="SCK08_000312"
     CDS             71664..72470
                     /locus_tag="SCK08_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200230.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72482..73702
                     /locus_tag="SCK08_000313"
     CDS             72482..73702
                     /locus_tag="SCK08_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1200231.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73692..75278
                     /locus_tag="SCK08_000314"
     CDS             73692..75278
                     /locus_tag="SCK08_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1200232.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75317..77455
                     /locus_tag="SCK08_000315"
     CDS             75317..77455
                     /locus_tag="SCK08_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1200233.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77823..78806)
                     /locus_tag="SCK08_000316"
     CDS             complement(77823..78806)
                     /locus_tag="SCK08_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEG1200234.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79184..80575
                     /locus_tag="SCK08_000317"
     CDS             79184..80575
                     /locus_tag="SCK08_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1200235.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80591..81631
                     /locus_tag="SCK08_000318"
     CDS             80591..81631
                     /locus_tag="SCK08_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1200236.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81650..82735
                     /locus_tag="SCK08_000319"
     CDS             81650..82735
                     /locus_tag="SCK08_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG1200237.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            82768..>83042
                     /locus_tag="SCK08_000320"
     CDS             82768..>83042
                     /locus_tag="SCK08_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1200238.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYV"
BASE COUNT        27175 a        13958 c        17568 g        24341 t
ORIGIN      
        1 atcgttatta tatgattaag aaggattgaa taaagaaaga agttgaaaac gatacctgat
       61 tacaaaaaaa taacttttat aagttctata acgttgatta tatttttaat tcttattctt
      121 ctcgtcgtct tggttccccg aatttttggt ttgactcctt atattattga ggacaaagaa
      181 atgagtccat attatgcaca aggaaatctg atatatgtac gaccgaaaaa tacaaatgag
      241 attttagtcg gagatattat tacatattat gaaaattcag gaaaaaaaat ctcgacgcgt
      301 cgcgtaatag ccgttgaaaa caaccacgaa gatttttata caaaagctga tacaaagact
      361 tatattgagc ctggagtagt aaaaaagaga aatattatcg gtagtcccat ctttcagatt
      421 ccatatattg gactagtcct ttcaaaaacg gcatttgcat ggattcaggg gttattcttt
      481 acgattgctt tttgcctaac aggaatcaca gcatggaata cctttgtaga gttgaaaaag
      541 aagcggagac gaacacagaa atttttgtaa gaatgttaaa aacataaatg tattccagag
      601 atccaacgac tcttctgaac atgaaacacg agaaaacact catgcagctt gtagatttag
      661 tcagttgttc gtataatgtg tgaagaggag gagtagtatg cgcaatgaaa tgatgcatcg
      721 tcctgtagtc aaagaagaac tattggattt tatgcgtacc cagcaaaaaa aattaccggg
      781 tgagctaggc aaactggaag aagaagctca tgtagcagaa gtaccagtta ttccccatga
      841 aacagttgta tttttgaaat ttttacttgg acagcttcag cctgaacgaa tcttggagat
      901 cggagcagct atcggttttt catcaagtgt catggccacg acaatcggag aaaacgctca
      961 tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa gctaaaaaaa actatgaacg
     1021 tctaggactg acagataaag tgactttgct tgaaggtcaa gcagcagata tcttgccgga
     1081 gttgtctggt ccgtatgact ttatttttat ggatagtgca aaatcaaaat atattgaatt
     1141 tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt ttgatggtag atgacatctt
     1201 ccaaggagga acgatcttgc tcccagatga agagattccc cgtggcaaac gagctatcca
     1261 tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat cccgatttga catcgacaat
     1321 cttgccatta ggtgatggtg tgatcttaat gacaaaggaa tcagaaacaa tcgagttgtg
     1381 atacatgatt atggcagcct gtagataatc gattgaatcg agggctcaat tcgaacttga
     1441 tgtaaaagtt tctatatttg taagtcaatt aaaggataca ggaaatctaa ataaacagga
     1501 gtagcatgaa gtcaatgtag ctcctgtttt caattgataa ccttagaaca gctgaaggag
     1561 aatgtctcgt tttaaaagat tgatttagta gttgggccaa aataattcat tatttgctaa
     1621 ttaaataact atttcgtatt gacagaagca atttaattat ctataattat tgatgttccg
     1681 ctgattcttg tcatagtagc tcagctggat agagcatccg ccttctaagc ggacggtcgg
     1741 gggttcgaat ccctcctgtg acgctaaaaa accgttagaa actgaggttt ctaacggttt
     1801 ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc aaataaaata aaaaaacatc
     1861 cttatcgagt acaattataa ggagcaaaaa aataaaactc atcagaaaag aggaagaaga
     1921 tatgacgata ctatctagaa ttttagtgac tctagttgca attgaatttt tttatatcat
     1981 gtatatcgaa acggtgagaa ctgattctga cacgacaagt agggttttta aaatgagtaa
     2041 agaagagtta agcagaaaat ctgttcaaac attgtttaag aaccaaggag tgtacaatgg
     2101 actattagga gtgggactac tttatggagc ttatctttct tccgcatcta aagaaatcac
     2161 aagtatgcta ttaatatcta ttttttttgt tgcattatat ggcagtttga ctagcgatcg
     2221 ttggatttta ctcaaacaag gtgggctagc tctgttggca ttgctttcta ttttcttata
     2281 aatatagaaa aaataagaat ttatctaaca ggaaactttg tgaaaaatat tactttatct
     2341 catgaaatat gatattctaa tgaatggtat tttagaaaat ttcatgaaaa aggagttaga
     2401 taggtgttca aaaattatgt ttcctcacta aaaaaagaat tttcaggtta tgatctttca
     2461 agatttcaga aagatctttt ggctgggatc acagtagctg ctgtagcatt gcctttagcc
     2521 ttggcttttg gggtatcaag cggagctgat gcggccgcag gtttgattac ggctatcatt
     2581 gccggtatcg ttattggcgg tttatcaggc ggtttttatc aaatatcagg tccgacaggt
     2641 gcgatggcag ctatcttgat gtctattgcg gcaacaaagg gaatgcaagg tgtattgctt
     2701 gctacttttt tagcgggtct atttttactt gcagcaggac ttttccgatt aggaacactg
     2761 acttctttta tcccagcacc cgtaattacg gggtttactt caggtattgc tatcattatt
     2821 gcattaggtc agatcgataa tttatttggt gttcactcgg aaggtacaaa cgttattgaa
     2881 aaactaagta gttatcaaac gataggattt ccgataaaca cagcctcttt gatcatggga
     2941 gggctagtca ttttagggat gtttatctat ccaaaaaaat gggcgaaacg aatgcctagt
     3001 tcgctgctgg ctatcatttt ggcaacagca gttatgctat tgttccattt accagtagca
     3061 actgtgggta agattccaca aacattgatc agtagtaatc gtttgaatat gggagatttt
     3121 agcttttctg ccttgcaaca agtggcagtt ccagctatta gcattgcttt attaggaatg
     3181 attgaaagct tgctatgtgg tgcttcggct ggccgaatgg caaataagcc cttagatagc
     3241 aatcaagaat taatggcgca aggaattgga aatctgttgc ttcctttctt tggcgggatt
     3301 cctgctactg cggccatcgc ccgtacaagc gtggccatca aatctggtgc acaaactaga
     3361 ttagctggta tgatacatgc agttgtctta ctgctctcca tgcttgtttt tgcgccagtg
     3421 atgtcacaga ttccaatgcc agcacttgca ggtgttttga ttgtgacggc ttggcgtatg
     3481 aatgaatggg aaacgatcaa ggagctattt actaataaat attggtcagc agttctattg
     3541 tttatcctta cgatgatttg tacagttatc tttgatttaa gtattgctat cgtgatcggg
     3601 gtgatcggcg gctgtgtatt ttttgttgtc aaaagcgctg ctatcacgat ttcattagaa
     3661 acgatcgatt ggcaaagaat ggggctgcca gaaacggaaa agctgaataa ctggacagta
     3721 gtgtatatta gtggtccact gttttttatg tcttcagaaa aactcaaaaa taaattggaa
     3781 gaactaaatc atcacgatgg tgtcattttc tccatgcgag gcgtaccaag tattgatttg
     3841 acggcacgtg cagtttttga tgaatttcaa gaaaaagcag agcaaagagg acaaacgatt
     3901 atctatactt ccttgcagcc agaagtagaa aaacaacttt ctcacttgtg ggaacataca
     3961 ttcacgcatc aacatcaaac agttgctcat gcgttgacag ctttacatgc gaaatatact
     4021 gcaattagcc attaaaacaa tgaaaaagaa cagacaagac tgtaaactga agattttatg
     4081 gtcttgtctg ttctttttaa tattgctgta aaagtgaagc tagtacttgc cagtcttgat
     4141 ggataacagg tacaagggag cgattgtaga aaatagctgc tggatgaaaa agtggaagaa
     4201 ttcgatagtt tttttctgac cattcgtagg catccttttg ttctgttaat tgtaagacag
     4261 gccctgtata gagttgacca tgatttttgg taaccgtata ttctttgcca agtaaacgtt
     4321 gaagaccaat gtttccaagt gtcgcaatta attttggctg gaccttcatc agttcgtaat
     4381 ccaatattgg tgcatgcgca aatacttcgg aacgagtagg tgtccgattt ggataaacag
     4441 tttctgactg ttgtgtccgt ttattgatcc gatgagtcac gcgatacggg cggctcctta
     4501 cagcactggt gatatacacc tcttctcgtg ttaagccagt tgaagaaagt gcctgcatca
     4561 attcttttcc agcttgaccg ctaaaaggaa catgcgtatc aatctctgtt ttccctgggg
     4621 cttctcccac gagcatcagt ttaggatgaa gaggtccttg cccagcgata aatccttcta
     4681 caggacggcc gttcattctt aaagaagctt cttgtatcag ttcttttggg tattccattt
     4741 atgctcctcc tttttctttt attataagaa taaaagaaac acaatgcgac tattttattt
     4801 ggtagaactg tgaggataga aaagcagaag tattgcttga atttttgttt tactttttga
     4861 ttttaaattt agttgactaa cggttgctgt tctggtaagc tagtactgtt gtaattgtgg
     4921 actccacagc tacaaccgct cagaacaagt aataagcaca gcatatgctg tcacttcgga
     4981 tggcgagtct aagtatatca gaaggaggtg ctttatagca tgtacgcaat tattaaaact
     5041 ggtggtaaac aagtaaaagt tgaagtggga caagcaatct atgttgaaaa acttgacgtt
     5101 gaagcaggag aaaaagttgt ttttgacgaa gttatcttag taggcggaga atctacaaaa
     5161 gtaggcgctc caactgttgc aggtgcaact gttgaaggta ctgttgaaaa acacggcaaa
     5221 caaaagaaag tcgttacttt caaatacaaa cctaaaaaac attcacaccg taaacaaggt
     5281 catcgtcaac catatacaaa agtggttatc gacgcgatca acgcttaata gcgtttgaga
     5341 aagaaggcct atatatgatc aaaggaacat ttaagcgaaa tgacgcaggt cagatcgttt
     5401 cattcacttt gaccgggcat gctgatgctg gtccttatgg aagtgatatc gtttgtgcgg
     5461 gtgtttctgc gttagcgatc agcactgtca acgggattgc ctcattagcc ggttttgagc
     5521 cgattgttga aatgaatgaa gaagaaggcg gttatcttta taccgaagtc acttcaggaa
     5581 tgacacaaga acaaaacaat attgctgaga ttctattaga aaatctttta ttaggattac
     5641 aatcgataga agctgaaaac tcagaatata tccaaattaa aacaattaat gataaatagg
     5701 aggtgcagac catgttgtta tctatgaatc tacaattatt tgcccacaaa aaaggtggcg
     5761 gttctacatc taacggccgt gactcagaat caaaacgtct aggtgctaaa cgtgctgatg
     5821 gacaaacagt tacaggtgga tcaattcttt accgtcaacg cggtacaaaa atctatccag
     5881 gtgtaaacgt gggtatcggt ggagacgaca ctttatttgc aaaagttgat ggcgtagtac
     5941 gtttcgaacg taaaggccgt aacaaaaaac aagtgtctgt ttatccagta gctcaagaag
     6001 cttaattgaa tcttatgatt aaagaggttg tgacaagact tttgtcacag ccttttttgt
     6061 ttataaaaat aaaagttgta gtggaggcta cgaagtttac tttgtctttt tacacttaat
     6121 atttttttgc caatttggaa tcaggatgtt ttgataacta tcgttttcaa ttgatggaag
     6181 ctatattgtt tgctatcagt gaaataatta tatggtaaac agtgttcttt ttcttgtttc
     6241 cattataaaa atcgtatcgg gatttttcgt tcttttaaat tgaacatttc tatctatccg
     6301 cgtgttatca acaaaacaaa atcaggtaaa tagttgacga aacagaaata aatacatagt
     6361 attatgtttt gaagcctaag tacaatagtc tatagaaaag gagcgatacc attggcaaaa
     6421 cgcattgacc gaatttatat gtatgtcaaa gaaaaaaccg ctcatttaac aaaggcagaa
     6481 tatgatcaag gagtaacaac acaggaaatt gctgaagtgt tagggattca gcgaacaaat
     6541 agcagtaaag atctgaatca gctagtgcgc gaaggaaaac tgataaagac agacggacga
     6601 ccagtacgtt acatatatca aacactcgtt acgcacgtaa agccagttac aaaacatgta
     6661 gtcagttaca aagaaacgcc ggtctctgaa gaaaaaaata cactccaaat agatactaag
     6721 gatatctttg caaaaatcat tggagctaac ggcagcatga aaaattcagt ggaacaggca
     6781 aaagctgcga ttttgtatcc gccaagagga ttaaattgtt tgattacagg acctaccgga
     6841 tcaggaaaga cttattttgc acacgcgatg tatcattttg caaaagcgca tcatgtaata
     6901 gatgaggaaa cggaactgat tgttttcaat tgtgctgatt atgccaataa ccctgagctt
     6961 ttgatgagtc atttatttgg gtacgcaaaa ggtgctttta caggcgcaga tgaagaaaaa
     7021 atggggatta tcgatcaagc ggatggcggt atgctgtttt tggatgagat tcaccggttg
     7081 ccgcctgaag gtcaagaaat gatcttttac tttatggatc atggaactta cagtcgtctt
     7141 ggggaaacaa cgaaatccca tgaagcaaat gtacggatcg taggtgcgac cacagaagac
     7201 ccaggttctt ctctattaga aacttttgtt cggcgtatcc caatcaatat caaacttccg
     7261 gcttttgaaa aacgtcctgc aaatgagaaa gtcgatttag taaaaatcat gattgcccac
     7321 gaagcaaacc gcacgcagcg aaagatttct ttgacagaag atgtagcaaa agcattgatc
     7381 ggcagtgtga cctatggaaa tataggacaa ttgaagtcaa atatccagtt agtatgtgca
     7441 cgaggatttc taaatcatat gcaatcaccc gaaatctcga tcacaatcga tgatttgaca
     7501 gaagggattc gtagcggatt gattcaatta gcaagcaacc gcagtgcaat ggcagagctt
     7561 tctaaaatat tggagccaaa gataactgta gcaccaaatg atacgatcat gaaaatccaa
     7621 tcggactcct atgagttacc ttataatcta tatgatatca tcggagataa agcagcttta
     7681 ctgaagtcgg atggtttgga tcaagaagca atcaatcatt tcatttctac agatatcaac
     7741 atccatctga agtctttcta taaagaccat ggtttttctt ttaatgcaga cagcaaatta
     7801 gcagaatttg tagatccgaa aatcatagaa gtgaccaatc agatttatgt aatggtcaaa
     7861 agatctttac catatgagtt tcagcaaaac tttatttatg ctatgagctt gcatatcagt
     7921 tcttttttga aaaaaatcaa cttaggagaa gaacggcata cgaatgataa catccgtgag
     7981 atggcttttg attttccaga tgaatatgct gttgcgaaag aagtgcgtag ttatatcgaa
     8041 acgtattttc aagtggagat tccagaaagt gaagactatt acttgaccgt tctactcgtc
     8101 tcgttacggg caaatcaagc aagcggcaga atcggtgtag tcgttgcagc tcacggaaac
     8161 agcacagcaa gcagtatggt ccaagttgtc caacagttgt tagatgcaga gaacgtacga
     8221 gcagtagaca tgcctttgga tatggatccg aaaacagcat tggaccgaat tgaacgcaat
     8281 gtacaagaag tagatgaagg aagcggggtc atactcttag tagatatggg gtcccttgcg
     8341 tcgtttaatt cgcaaattca aagagacact ggaattccag tacgtactgt tgatatggta
     8401 acaacttctc tagttttgga aactgtacga aaggtctcag tattagggac agatctagat
     8461 atgttgcatg aatcattgaa aaattttcga ggttacgcag aaatatcttc tgtagaatct
     8521 gttcaggaag caaaacctat tcataagaaa aaagctgttt tggctatctg cgcatctggc
     8581 gaaggtacag cacaaagaat caaagaattg atcgaacgag cggtaaacaa gcgtcaagaa
     8641 acagaattag aagttctagc tttgtcgatt gtggaagtga aagaagaact gccagttatc
     8701 caaaaagaat atcagatcat agctactact gggattactg atttgaatat ccaagcccca
     8761 tttattcctt tagaacgttt tattgatcaa aatatcgaag tcattttgga tcagttgctg
     8821 atggaatcag aattggagga aactgaattc atctccctag atgaagagtc agctaaaaat
     8881 acgtgtgttg agtttatctc tgataacttc atctttatta atggtagtaa gttaatcgat
     8941 ccaatgtggc aattttctac acaaatctct cagacgacgg gaatcggcga tgaagaatat
     9001 ggattcaaaa tcaacctagt gatgcacaca gcaggaatga tcgaacgtat tattcggaat
     9061 gaaccattga ctgtggaaga aaacgaattg acaaatacaa cgaatgatcc tctttatagc
     9121 caattggcag cctcagttgt tctgttggaa gatcagataa aagtaaaagt gccgatagaa
     9181 gaaatgtatt atctgctacg tctagtccat aatcagttag ataaaaaaga atacactgtg
     9241 ccatgaatac acaaaaacga tacactttta tagtgtatcg tttttgtgta ttggttaaga
     9301 atcctttaaa ttcaattagt gtatcgtaaa aaagttggca cgctttttgc ttatagtagt
     9361 gtgtgctagg aggtggttgt tcacacagaa atgatagggg tttctgtgaa gtagtgagga
     9421 agaaggagtt ttgcagatgg atattcgatt ggcacgtata gatgatcgtt taatccacgg
     9481 gcaagtcgca acagtttggt cgaagcaaac aggtgttgaa cgtatcattg tgatcagcga
     9541 tgtagtcgct caagataaat tgagaaagtt tctactaaaa gaagctgcac cgccggggat
     9601 cacagcaaat gtaataaccg tgcagaaaat gattcaaatc tatcatagtg gattattatc
     9661 acgagcaaaa gtgatgcttt tattcaccaa tccccaagat gttgaagcgg ttgtccgctg
     9721 cggtgtacag ttgaagtcgt tgaatatcgg cggtatgagt tttactgaag gcaaatcaat
     9781 gattaccaat tttgtttcag taaatcaaca agatattgat tcctttcgtt acttgaacag
     9841 tcaagaagtg gagttagaga ttcgaaaagt acctgctgac aggaaaatcc ttttgatgga
     9901 actactagcg aaaatcaaaa aggtgtagca agaatggttg ttggaaaagg tcaaataatt
     9961 ctgttgtaat ggttagcaat tattaatatt tttgaatttg ttacaacaaa agtaaatagg
    10021 aggtataaat atggtaggga ttatcctagc aagtcatgga caatttgctg aaggaatctt
    10081 acaatccggt tcaatgatct ttggcgaaca agaaaacgtc aaagccgtga ttttgaaacc
    10141 aagtgaaggt ccagatgact tgagagccaa attggaagaa gcggttgctt cttttgataa
    10201 tcaagacgaa gtgttattct tagttgattt atggggcgga acaccgttca atcaatcaaa
    10261 cacattattc gaagagcaca aagataaatg ggcaatcgtc agcggtttga atttaccaat
    10321 gttgattgaa gcctacgcat cacgcttttc aatggaatct gctcatgaaa ttgcagcaca
    10381 catcattgaa acagcaaaag acggtgtaaa agtaaaaccg gaagaattag aaccggcaga
    10441 agcaccgaaa gcagcagttg aagacgcaca acctaaagga gcacttccag aaggaactgt
    10501 ggtcggagac ggaaaaatca aatatgtatt agcacgtgtt gactcacgtc tgttacatgg
    10561 acaagttgcg acagcttgga caaaagcagt gcaaccaaac cgtattatcg ttgtttcaga
    10621 tgcagtatca aaggatgatc tgcgtaagag actaatcgaa caagctgctc ctccaggtgt
    10681 aaaagcaaat gttattccta ttagcaaaat gatcgaagta gcaaaagacc ctcgttttgg
    10741 aaataccaaa gcattgcttt tatttgaaaa tcctgaagac gtattgacag ctgttgaagg
    10801 tggcgtagac atcaaagaat taaatgttgg gtcaatggcg cattcggtcg gtaaagtcgt
    10861 tgtaagtaaa gtattgtcta tgggacaaga agacgttgaa gcgtttgaga agcttgaaca
    10921 aaaaggtgtg aaattcgatg tacgtaaagt acctaatgat tcacgcgata acatggatga
    10981 tattttgaaa aaagcaaaag ctgaattagc aaaagcgtaa taaataatat taaaatttat
    11041 aggaggctta tcatgtctat tatttcaata attttagtcg tattagttgc ctttctagct
    11101 ggtatggaag gtattcttga tgaatttcaa tttcatcaac cactagtggc atgtacattg
    11161 atcggtttag ttaccggtaa cctcgaagct ggtatcgtac ttggcggtac gcttcagatg
    11221 atcgctttag gttgggcaaa tatcggtgcc gctgtagcac cagatgctgc attagcatca
    11281 gtagcatcag caattatttt agttttaggt ggacaaggtg ttaaaggtgt tccttctgcg
    11341 atcgcgattg ccgttccttt ggctgttgca gggttgttct taacaatgat cgtacgtaca
    11401 atcgctgttc caatcgttca tttgatggat gcggctgctg aagaaggaaa catccgtaag
    11461 gttgaaatgt ggcacatcat tgctgtatgt ttacaagggg ttcgtatcgc ggtcccagca
    11521 gcagcgttgc tatttatccc agcacaaact gtgcaatcat tccttgaatc aatgccagca
    11581 tggttaacag atggtatggc tatcggtggg ggaatggtcg tagctgtagg ttacgctttg
    11641 gtaatcaaca tgatggctac aaaagaagta tggccattct tcgttattgg tttcgttgta
    11701 gctgcaattt ctcaattaac attgattgct ctaggtgctt taggtgttgc attagctctt
    11761 atttacttga acctttctaa aatgggtggt tcatcaaata atggaggcgg aagcaactct
    11821 ggtgaccctt taggcgacat tttgaatgac tattaatctt gaaggaggaa aataaaatgg
    11881 cagaagaaaa aatcaaatta tcaaaaaaag atcgtttagc tgttgcgtgg cgctctacct
    11941 tcattcaagg ttcatggaac tacgaacgta tgcaaaacgg tggctgggca ttttctatga
    12001 ttccagcaat caaaaaatta tacaaaacaa aagaagaacg ttctgcagca ttaaaacgtc
    12061 acttggaatt ctttaataca cacccataca ttgcttctcc aattttaggg gttacattgg
    12121 ctctggaaga agaacgtgcg aatggcgcgc ctgttgatga tgtagcgatt caaggggtta
    12181 aagtaggtat gatgggtcct ttagctggtg ttggtgaccc agtgttctgg tttacagtac
    12241 gtccaatgct aggtgcacta ggtgcttcat tagctatggg aggaaatatc cttggaccaa
    12301 tcatcttctt cttaggttgg aacattattc gttgggcatt catgtggtat acacaagaat
    12361 ttggctacaa agctggttct aaaattactg atgacctttc tggcggattg ttacaagacg
    12421 taacaaaagg tgcttcaatt ttagggatgt tcgtattagc tgcccttgta caaagatggg
    12481 tatcaatcaa attcttacca gttgtatcaa ctgtaaaatt agataaaggt gcttacatcg
    12541 aatgggataa attgccttca ggtggtgaag gaatcaagag tgcatttgaa caagttaata
    12601 atggcttagc cctttctcct gaaaaggtaa caacattaca agacaactta gaccaattga
    12661 ttcctggttt agctgcatta ggattaacat tgttatgtat gtggttgctt aagaaaaagg
    12721 tttctccaat cgttatcatc cttggtttat tcgtagtcgg tgttgttgga cacgtcatcg
    12781 gacttttata atccataaaa atatagctcg ggcgttgtcc cgagcttttt ttatatagta
    12841 tactgttcat aaacaaatat ctggagggag aaaatggttc aatcaatcaa tacaaaagta
    12901 gacttggtaa ttgatgctac ttcgtttacg ggtctgacag attacgggca aatcatgata
    12961 ggtgataatg gttttgagtt ttaccatgcg cgagatagac gcaaatatat ccaaattcca
    13021 tgggaagaag tggattatgt actagcatca gtcatgctaa agggaaaatg gattcctcga
    13081 tatgccattc aaacgaaaaa aaatggtaca tacacatttt cttccaaaga acctaaaaaa
    13141 gtgcttcgtg cgatcagaga atatgtagat gaagatagaa tggtacaatc attgagtttc
    13201 tttgatgtag tgaaacgtgg tattcgtagt atgtttaaac gcaagtaaaa aataaagaaa
    13261 acttctaaga ggaggttttc tttatttttt tactttgcag aagaaaatat gaaatagcaa
    13321 aaccaattat ttgtcccctt taaatcccat atgctaaaat tgattagcaa agaaggaggt
    13381 tatattatga gttggttatg gacattgatt gttggagccg tgatcggagc aattgcaggt
    13441 gcgatcacca gtaaaggaaa ttcgatgggc tgtgtaacga atatcattgc tggactagtc
    13501 ggttcaagta ttggacaagc gattttcggc gaatggggac caaaagctgc aggtatggca
    13561 cttattccat ctattttagg cgcagtgatt ttggtagcag ttgtctcatt tttctttgga
    13621 aaacgatcat aagtttttac aaaatagtaa actaaaagga gttgcagcga gactgtgact
    13681 cctttttggc tttatttatt taaaataaaa agaagttttc cggaaatttt tatttaactg
    13741 tcgtgtacga agaaaagttt gctataatgg aaaaaagcgt gcaaagaaag agggagtttc
    13801 attatgatgc tacgtgtaga aaaattgcgc aaaaaaatgc aagaagaaaa tttggattca
    13861 tttttggtta ccagtccgta caatcttcgt tacttaacga actttacagg tacgacagga
    13921 ttagctgtta ttactttgga aaaagcattt tttattactg attttcgtta taccgagcag
    13981 gcggcagcgc aggctcaagg gtttgaaatt attaaaaatg tcggacctat ttttgatgag
    14041 gtagcaaact tagtacgcaa agaagaactg cactcattag cttttgagga aacaacagtc
    14101 tcatttttag aatattcggt tttagaagaa atcatcgatg cggaattagt acctgtttca
    14161 cagatgatcg aagaactcag agaagtgaaa gatgaagaag aaatcgctat tatcgaaaaa
    14221 gcatgtcata ttgcggatat ggcatatgat cacatcctga aaatgatcca accgggcatg
    14281 actgagattg aagtcgcaaa tcaactagac ttttatatgc gttctcttgg cgcttcaggt
    14341 gtttcttttg atacgatcgt tgctagcgga ttgcgttcgg ctatgccgca tggtgtagca
    14401 agcaaaaaaa tcatcgaaca aggtgacttg atcacattgg actttggttg ctattacgaa
    14461 gggtacgttt ccgatatgac acgtacattc gctattggtg atcctgggga aaaattaaaa
    14521 gaaatttatc aaatagtctt agatgctcaa ttagcagtta ttgatgctgc aaaaccagga
    14581 atgactggtg tccaattaga tgcagttgct agagattata tttcaaaaca tggttacggg
    14641 gaagcatttg gacattctac aggacatggg atcggtttag agatccacga aggaccaaac
    14701 gtttcttctc gtgcagaaaa gcaatttgta ccaggaaatg tgatcactga tgaaccaggg
    14761 atctacttac caggaatcgg tggagtacgt attgaggatg acttgttgat cacagaagat
    14821 ggaaatcgtg tgttgactcg ttcaccaaaa gaattgatca tcttatagtt tcatcaatag
    14881 aagaagtaag cgtaaaaatc agtgttcaca gactttgtta tagaaaatca ctcgatttaa
    14941 tgatacacta tagaagaact tttaagctgt aagagataat aaagttgggt gttttctcca
    15001 attcatagca gtttgcagct gatcggcggc agttttctta ccacgtgatc actatgccgc
    15061 atcatagaga attcggatgt atctcgtgct taggcaaagg gaggaataaa taaatggcag
    15121 atgaaaaaaa tttgatttta aacgcaaacc aagatttagg agagatcgtg atcgctcctg
    15181 aagtcatcga agtaattatc gggattgcag cttctaaagt agaaggcgta tacggaatgc
    15241 gcggaacttt tgccagcaat gtcactgaat tattaggacg tgcagctcac ggaaaaggtg
    15301 tttacttagc aaacgaggaa gatggcttga aagtagatct ttactgttat ctagaatatg
    15361 gtgtttcagt tcctaaagta gcaatggaaa tgcaggaacg ggtcagccag caagtccttt
    15421 tcatgacgga tatcgattta tctgaagtca atatccatgt agtagctgtt gttcctgaaa
    15481 aattagaaca accaacaatc gaagaacttt ttgaagacga agaggaagag aatgaataaa
    15541 gaattatcaa gacacgagat tcgggaaatg gcattgcagg ctttatttcc attagacttc
    15601 aatgcagacc taacaaaaga agatgcgatt ttcaacgcaa tcgaattaga ccaccgcgac
    15661 atgatcaacg aagatgaatc agagtttgtt cctgtttatc ttgatacgct agttggcggt
    15721 gtctgtgcaa aaaaagatga attggataaa gtgattgaaa aacatttaaa aaacaattgg
    15781 cacatcaatc ggatctcaaa aatggatttg gtgatcttgc gtatcgcgat ttttgaaatg
    15841 acttatgtga cagatgtacc agcggctgtt gctttaaatg aagcaatcga gttggcaaaa
    15901 acatttagtg atgaccgttc acgtaaattc gtcaacggtg ttttatccaa tgtattgaaa
    15961 gaattagaag ctggggcgta agtcctagct tttttctatg aatcatcata aacctgttat
    16021 aaatcgggaa aattctatgg tagaatgaaa acacaaaaaa tatctgggag tggttagatg
    16081 gcagaattga ttaacggaaa agaattagct gaaaaaatgc aggcagagat tgctgaaaaa
    16141 atccaaaaat tgaaagataa tggtatccat cccggattag tcgttttatt ggtaggggaa
    16201 aatccagcca gccaaatcta tgtaagaaat aaagaacgtg tagcaaaaga aatcggaatc
    16261 cattcattgg tagagcgtta tcctgagacg atttctgaag acgaactact tgcagaaatt
    16321 gaaaaataca atcaagatcc tagttttcat ggtatattgg tccaattacc attgccaaag
    16381 catatagatg aagaaaagat cctgttagct atccgccctg aaaaagacgt agacggtttt
    16441 catccgatga atttaggcag actccttgcg ggtaaaccgg acaagattcc ttgtactcca
    16501 tacggcatca tgaaaatgtt tgaagcttat tctatcgatc cggcgggtaa acgagcagta
    16561 gttatcggac gaagcaatat cgtcggtaaa ccgatggctc agctactctt gatggcagat
    16621 gcaactgtta ctgttgctca ttctaaaacc gaaaatctgg ctgaactggc acaagaagca
    16681 gatattttag tcgtagcaat aggacgtgga cattttgtca caaaagaatt cgtaaaacca
    16741 ggtgctgtag tgatagatgt agggatgaat cgtgatgcta acggtaagct gatcggtgat
    16801 gtgaaatttg atgaagtgga gcctattgca ggctggatca ctcctgtgcc aaagggtgta
    16861 gggccaatga ctatcacgat gctgatgtat caaaccgtaa aatctgccga agcttttgga
    16921 gcaggtgaat agatggcaca agaatattta actgtcacta cattaacgaa gtatttgaaa
    16981 agaaaatttg atgccgatcc ttatttagaa cgcgtatatt taacaggcga aatatcgaat
    17041 ttccgtttac gtgccaatgc ccatcaatat ttcagcttaa aagatgacca tgcaaaaata
    17101 tctgctatta tgttcaaagg agcattccaa aaattacgat tccagccaaa agaaggaatg
    17161 aaagtccttg tcgttgggcg tatttcatta tatgaagcgg gaggttctta tcaaatctat
    17221 gtagagcaca tggagcctga tggaataggt gctttatatc aagaattgga agaacgaaaa
    17281 gaaaagctga tgaaggaagg cctttttcaa gggcctaaaa aaatgctgcc acgtttccca
    17341 aagcgaatcg cggtccttac cagtccaagc ggagcggtta tccgcgatat catcacgacg
    17401 gtgaaaagaa gatatccaat tgtccaattg gttttgttcc caacagttgt acaaggaaac
    17461 caagcagcgg acaatgttgt caggaatatc cagagagtag aagagatggg caatttcgat
    17521 acaatgatca tcggtcgtgg gggtggttcg attgaggatt tatggccgtt taatgaagag
    17581 cgggttgctc gggcgattta tcaagctaat acgccggtga tttcatctgt aggacatgaa
    17641 acagatacca cgatcgctga tttagtagca gatgttcggg ctgccactcc aacagcagct
    17701 gcagaattag ctgttcctgt tttgtcagaa gagttgatgc gcattcaaga acggcaagct
    17761 agattgcagc aggcgtttgt acgccagatc cagcgcaaac aagaacggtt cgagcgtgct
    17821 cgacgttcct atgtatttcg gcagcctgaa agattgtatg aaggtcagac catcaaatta
    17881 gaccagttga aacagcgatt atatcaagct acacagcaaa tttatcatgc gaaagaaaaa
    17941 caaacgatcg cattaagcca ccaattggaa caagtggctc cgctttatcg agttaaagca
    18001 gctcgccaag aaactgctta tctagaaaaa cgcttagaag agaaaatcca acaatatatg
    18061 aaacaacagc agcagcgttt tgctcaagct gttcaatctc ttgatttgtt gagtccattg
    18121 aaaatcatgg gaagaggcta cagctatacg acgttaaatg atcaagtagt aaaaagtgtc
    18181 accgagattc atcaagggga tttgctgacg atccattatc aagatggaat cgttaaagga
    18241 caagtacaag agatagacaa aggagaataa aaatatggaa aatccaactt ttgaagagtc
    18301 actgcaagaa ttagaaaaaa ttgtcatgca gttagaacaa ggagacgttc cattagaatc
    18361 agctctagat tcgtttaaac gaggaatgga attaagcaaa cattgccaag atacattagc
    18421 taaagcagaa gagaccttga cgaaaatgat gacagaatca aatgaagaag ttgattttga
    18481 cggaaacgag gaaagctgag atgagattaa ctgacttttc agctattcat ctacctgctg
    18541 tagaaaaaga aatcttgtct tttttagatg agcatacaac ggatagagga ctctatgatg
    18601 cgatgagtta ttcagtgaaa gcaggcggga aaagaatacg tcccttatta cttttaacgg
    18661 ccgttgcttc ctttgatgag cctattgatg ttcccgtcta tcaagtagca gctgctttag
    18721 aaatggtgca tacgtattcc ttgatccacg atgatttgcc tgcgatggac aatgatgatc
    18781 ttcgtagagg aaaacctacc aatcataaag tattcggcga agcattagct atattggccg
    18841 gagatggttt gcttacagga gcattccagc taatcagtat ggcacactta ggtaattctc
    18901 ctaagcttct gctgcttcag caattagctg tttgtgcggg atcacaaggt atggttgctg
    18961 ggcaagctgc agatatcgag ggcgaatcga aaaagctttc tttagaagaa ttagcattta
    19021 ttcatgaaag aaagacaggg catttgattc gttatgcctt gctggctggt ggcatattag
    19081 cgaagcaacc tgaagaaatc ttattgctac ttcaacgatt agccgaacat ttaggtttag
    19141 cttttcagat aagagatgat ctactcgatg tgattggtac aacgaaaaca ttagggaaaa
    19201 cagctggtaa agatgaacgt atggaaaaaa atacttaccc gcgtttatta ggcctagaaa
    19261 agacacgtga ggcgttggag attgagctgt tatccgccaa caagatcata gacaaattag
    19321 aagaggacgt tttttctttt gatggcgggc tgatgcgcca gatgatcaag caatttgatg
    19381 tggagaaatg agggaaagaa tggaaaaaga acgcgtagat gtattagcgg tcaaacaagg
    19441 attatttgaa acaagagaac aagccaaacg ttcagtgatg gctggtttga tttacaacga
    19501 aaaaaacgag cggtttgata aaccgggaga aaaaattcca gtgagtagtg aattaagagt
    19561 caaaggtaaa aaacttcctt atgtttctcg agggggatta aaattagaaa aagcactcaa
    19621 agcatttgat ctatctgttg aaggaaaaac attgttagat attggtgcat ccactggcgg
    19681 atttactgat gcagctttgc aaaatggcgc aaaaatgagt tatgctttag acgttggcta
    19741 caatcagcta gcttggaaaa taagacaaga tcctagagtc gtcgtaatgg aaagagtgaa
    19801 ttttcgttat gcaaaacccg aagactttac cttaggaatt ccagaagttg ctgttatcga
    19861 tgtttctttt atttcactaa aactgatgct gcctccatta catgctattt taaaagaaga
    19921 gggagaagtg attgctttaa tcaaacccca attcgaagca ggcagagaag ctgtagggaa
    19981 aaacggaatt gttcgtgacc ctcagaccca tcaaaaagtt ttagaagaca ttttgtcatt
    20041 tgcggcacaa ggtggatatg atgtgctgga attaagttat tctccgatca cggggggaga
    20101 aggaaatatc gagttcttgg ctcacttgag gaaagtaccc gaaagcggaa caatcaattc
    20161 agctattaat atggcagaag tggtttcaaa tgcacacgaa cagtttgacc ataaataaat
    20221 tgtaactgtg aaaatcaagt gagaagtatg cggattcttc tttattatac caaggaaata
    20281 agctatcatt gattaagaag aatatgctaa ggagcgaaga cgtaatgaga aaaaaagatc
    20341 ggcatcgttt gattactcga ttgttgagtg agcaagatat ccgaaagcag gaagaatttg
    20401 ttgaaatcct gaaaagcaaa ggaatatctg ttactcaagc tacgatttcc agagatataa
    20461 aggaactgaa actgatcaag gtaccggctt cagatggcgg ctaccgttat agcataccag
    20521 ctgaaacaaa cgaagatgta aatactaaat tggaaaaact attaaaagac gctttcgtgg
    20581 cagttgacca aatggaaaaa tttgttattt tgaaaacatt gccgggaaat gcttcggcag
    20641 cagcaaattt gattgataaa cgttacaaga aagaattatt ttcgattatc aatgatgacg
    20701 ataatgtttt gatgattaca cgaactgaag accaggcagt tttattgaag aaagacttcc
    20761 tgcattacct ttaagttgag gagcgaatgt gatgctacaa gaaatcagta ttactaactt
    20821 tgcgattatt ccggaattgc ggctttcttt tcacgaagga atgaccgcgc tgaccggaga
    20881 gactggtgcg ggtaaatcca ttattatcga tgctttaggt ctgttagcag gaggaagagg
    20941 ttcaagcgat tatatacgtc aaggagcaga aaaatgtgtg cttgaagggt tgtttgaact
    21001 accaaaacaa gaaggattct ctgaactgat ggttgaatta gggatcgaaa cagatgagga
    21061 caatctgatt gttcgacggg atatgtcctt aactggaaaa aatgtttgtc gagtgaatgg
    21121 tcatattatc acattagcta atctgagaag aatcggcagt tatttagtag atatccaagg
    21181 acaaaacgaa catcaggaac tgctccagcc tgaatcacat cttgctttat tggatcgttt
    21241 tggagatgta gctttccaac agaagaaaaa gagttatcag caagaatata tctcttatcg
    21301 tgagctggaa aaaagggtac gaaaagtgca gcaaaatgaa aaatcctatg ttcagagaat
    21361 agatatgctc catttccagc aagaagaaat agctgctgct gatttgcaag tcggagaaga
    21421 agagaaatta aaagaagaaa gagaaaaact tagcaattat caaaaaattg ctgatggatt
    21481 agctgcaggt tacggagcac tgacgaataa tgaacaaaac agtgtagatg gtgttggctt
    21541 ggccgtttca gaactgcaag ggatcgccca tctagatgtg gagtatgaag caatttatga
    21601 aaatatccaa agtgcttact atttgctgca agatgcaatc ggcgatatga gtcgtcagat
    21661 tgatttattg gagttagatg agagtcgttt agaagaagtt acgcaacgtt tagaattaat
    21721 ccgacagttg aaacgtaaat acggcgaatc cattgagtca atattggctt actatgacga
    21781 aatcaccgaa gagctagctt cttctgattt ttcagaaggt caattggaca aaatgaaaga
    21841 ggaactagag caaaaagagc ttctacttca gcaacaagcc gcagatcttc atgaagcgcg
    21901 taaaaaaatc gcaaaagaat tggaaaaatc cattctgcat gaattgaaaa gtctgtatat
    21961 ggaaaacact gagtttgagg tacgtttctt aaaagaagaa aatagacaat tgaatcatga
    22021 tggatttgac caaatcgaat tttacattac gaccaatcca ggagagccac tgaagccttt
    22081 ggtgaaagtc gcttctggtg gcgaactgtc tcggatgttg cttgctttga aaactatttt
    22141 ttcttctgag caaggtgtga caagtattat ttttgatgaa gtggatacag gtgtgagcgg
    22201 aagagttgca caggccatcg cagataaaat ttctcaaata tcgaaatact ctcaagtttt
    22261 gtgcatcact catctgcctc aagttgctgc agtcgccgac tatcaatatt atattgtgaa
    22321 agcagtgatt gatggacgta cgcaaacttc tgtttcagag ttgaaaacaa aagaaagaga
    22381 agaagaaatt gcacgtatgc tggctggaag tgagattaca aaactcacac tcgaacatgc
    22441 aaaagaacta ttgaaactcg caaaaaggac ctgagccacc tcgcagtggt tcaggtcctt
    22501 ttcagcagct gatagctata ttttattttt acatgacact cattacagca gtcaatacaa
    22561 cagatccaat cgttaaaatc agcattgtcc aaatgacaaa ttttgttact ttagcaaaag
    22621 tacttgtttg ttttttttca cgcattgttt tcaacctcgt cattaattag ttgattcaag
    22681 tgtacaatct tttttccatt tccacaagag ggctttttta taaaaacttc ccttttttta
    22741 aataataggt agttacgata caaataaata atgttaatag aactagccat ataaaggcat
    22801 catccctttc agcaataggt aacttaatgt tcattccata gatactgccg acgattgtcg
    22861 gtatagttaa aataatcgtt aaagaagtta aaattttcat aatattattc agattattcg
    22921 atacgattgc tgaaaaagtc tgattcattt gttctactag ttttaattga atgcgagaag
    22981 tagtcattgc ctgctctgtt tccaccaata catcacgtaa atgatttttc aaagcatgat
    23041 tttcttggaa aagtttattt tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg
    23101 cttctctgaa ataaacgagg cttttttgga tatccatgat ttgataaagt tgtttgtttt
    23161 cagtggatac ttgaagtttg gcttccaaag tatcgcattg agattgaagc gtagctaaat
    23221 aactgttgta agagagtgtg acctgccaaa tgaattgaaa gaagatattg agattaggcg
    23281 aaatatcact ttttggaaag gtctggctaa agactttctt taaaaacagt ggttcatgat
    23341 tcgtaattgt gataagcttc tttttgggcg tcaaaataat agagagcggg tatgtatcca
    23401 cttgaaaata accgctagga cttgttttag caaaaggata ttgtaacaat aataacgctg
    23461 gctttgtcaa ctttgattga ttgaattctt ctgtacggga attttctgca tcgtccaata
    23521 tggaagtgat gtaatctgac ggaatttcaa attgatgtgt cagctgatga atctcttctt
    23581 gattaggacg ttctacacaa taccagatcg tttggtcagt ttcctttccg gtattacgca
    23641 gtttctcatt atctaatgta aaatatttga tcatgatttt cctcctgttg cctgtttctt
    23701 ttattataac ggatagggaa ttggaataca tggaatgtgt ttgtaagata tagaatataa
    23761 ctgcataaat ttaaataaaa tgaaacagtt gtgtaaagta tatacagtat acttgaagca
    23821 acgataaaaa aggagtttac gttcatgggc acattcacac cacttatctt gttttggtat
    23881 atttacccgg taatcgtttt gtttggttgc caatttcttg taaaattatt ttcgttaaat
    23941 cgtcgtttta aaataaaagc ccctgatttg gcgattcctt ttttgtgggg cggtatgcat
    24001 gcattgtcta gaaatacagg gacaatttct atcttaccgt ttttctttat ttctgttttg
    24061 ctaatgggaa tttgtattgc tgttttccaa gcatattatt atgaagaaat tatttatccg
    24121 cgttatttca agatgacttg gcgtttggtt tttctgttga caatcgtctt gtacgctgtc
    24181 ttgatcattg tgaacatact ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct
    24241 tttaaaaggc tttttgcagc ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt
    24301 cccggatttc ccagaaaagg tggtagaaag tgggggattg tggtagacta attttagtta
    24361 gtggaggaat ggggggcttt caatgttgat gggtgaattc caacataata ttgatgcaaa
    24421 aggacgttta atcgtgccct caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac
    24481 acgcggattg gatggctgtc ttttcggcta tccgatgagc gaatgggaaa atctggaagc
    24541 taaactgaac gaaatgccgt tggctaaaaa agacgcacgt acttttgtgc gattcttcta
    24601 ttcggctgcc actgaatgcg aacttgataa acaaggacga atcaatatac ccagcacgtt
    24661 aagaaattat gcagctttga caaaaggttg cgtagtcatc ggtgtatcta accgaattga
    24721 aatttgggat gagacacgat ggcaagattt ttctgcagca gcagaagaga actttgacga
    24781 aattgctgaa agtatgattg atttcggact ataagaatag gaagaggctg actaatgact
    24841 gaaacatttc aacactatac agtcatgttg aaagaaacgg tagatggatt gaacatcaaa
    24901 ccggatggcg tttatgtaga ctgtacactc ggcggtgccg gacatagtga atatttatta
    24961 agccaactaa gttctaaagg gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat
    25021 cacgccaagc tgcgtttagc ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg
    25081 aacttcagag aattagaaga acgcttgagt gagcatgtag ataaggtaga cggcattttg
    25141 tatgatctag gggtttcctc tcctcagcta gatgaagcag aacgtggatt tagttatcat
    25201 caagatgcac cgttggatat gcgcatggat cagtcagctc ctttatccgc ttatcacgta
    25261 attaatgaat attcctacca tgaattagta aagattttct tccgctatgg cgaggagaaa
    25321 ttttccaaac aaattgctcg tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca
    25381 actggtgagc ttgtcgaaat cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc
    25441 gggcatcctg caaaaagaat ttttcaagct gtgcggattg cagtaaatga cgaactcggt
    25501 gccgaagagg cctctttaga acaggcaatc cggttattga aagtcaatgg aagaatcagc
    25561 gtaatcacat tccattcatt ggaagacaga attgtcaaaa gtatgtttaa agaatatagt
    25621 acggtacaag atctaccacc gggacttcct gtcatacctg acgaattcca accagaactg
    25681 aaagtcatta ctcgtaaacc aatccttcct tcagaggaag agctaacaga aaacaatcga
    25741 tcacgcagtg caaagttacg tattgcagaa aaaataagaa tgaaagagga gtagaatgat
    25801 ggcagaatta aagaaaatgc aagattacga atatgattta cctgaattgg tggatcagcc
    25861 ggaacaacaa atgcctgatt cttcttcgaa tcaagaaatc attcttccac aatcaccggc
    25921 aaaacgatta aagcagattt ctgctttgga aaaaattgct gtggcaagcg tggcgattgc
    25981 tattgttgct ttatgtgttt taacagttat gctgcgcaca aatatcagcg gagctgaaaa
    26041 agaaatcacg tcaatacaaa cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga
    26101 acaagaaaaa aacgaacttt ctcgtactga acggattaaa caaatcgcag aagaaaaagg
    26161 attgtctatt aatgacgaca atttaaggaa agtgaagtaa atgagtctta aaaataaaat
    26221 tcggcggttt atggaaaaga aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat
    26281 cattttattt gctacgagta ttggattgtt ctttttattt gccgtccgtt tctcgtatat
    26341 cgttattgga ggtcatgttg ctggtacctc attagctgaa aaaacaaaac agttgtacca
    26401 aggaagtgaa gtagtaaaag caaaaagagg aacgatttat gatcgtaacg gtgtggcgct
    26461 cgcagaagat gcctcctctt attcaattaa agcgatactc tcaaaaacat atacctctgg
    26521 ggataaaaaa ctgtatgttg aagaaaagaa ttttgataaa atagcagaaa tcctgcataa
    26581 gaatctttct atagacaaaa aagatgcact gaatatttta gaggacggcg caaaaaaaga
    26641 gctgtatcaa gttgaatttg gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa
    26701 catcgaagcg gacatgaaaa aagaaggtgt cgctggtctt tattttgtcg accatcaggc
    26761 tcggatgtat ccaaatggcg tcttttcttc tcattttatt ggatacgcag ttccagacaa
    26821 agatgagaat ggcttagttg gtaaactagg tttggagtct gcttacaatg atatcttgag
    26881 cggtaaagat ggtaagatta tttatcaaaa agataatttt caaaatcctc tgccaggtac
    26941 agtggcagaa gaagaaaaag ctgtcgacgg acaagatatc tacacgactt tagacagcag
    27001 actacaaagt tatttagaaa cactgatgga tcaagtaaat gaagaatatc agccagaaga
    27061 attgacagca gtcctgatga aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc
    27121 tactttcaat cctgaaacaa tggaaggatt gactggaaaa gatgctatct ggcgtaattt
    27181 cttggtacaa gatagttatg aaccaggatc aaccatgaaa gttttcacta ctgcagctgc
    27241 tatcgaagaa ggagaattca acgagaatga aaccttccaa tctggaaaaa tccaagtaga
    27301 agatgctacg atcaatgacc atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc
    27361 tctttcatgg tcaagtaacg tcgggatggt catactggaa cagcgattag gcggacgatg
    27421 gtataactat ttacaaaaac tgggatttgg acaaagtact cattctggat tagatgatga
    27481 agtaaacggt gctttaccta cattaaatat tgtcgaccga gcaatgagtg cttatggaca
    27541 agcggtaggt gtgacgaatt tccaaatgat gaaagctttc acttctattg cgaataacgg
    27601 tacaatgatt caacctcgat atatcagcaa agtggtggat ccccaaactg gagaagagcg
    27661 aacaactcaa acagaagttc tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg
    27721 tgaatatatg agagatgttg tcgaaagcga aaactacggt agtgcttatg gtgtatatag
    27781 tgtacctgga tacaatgtat cggcaaaaaa cggaacagcc caaatcgctt ctgatacagg
    27841 gggatatcaa acaggagata cagcctacct gtattcgatt gtggagatgg ttccttctga
    27901 agatccagac tatgtcttgt atctaacaat gaaacaccct aagacatatg atcgaatggc
    27961 attggctaaa atcgcaaatc cattgatgaa acgggcaatg gattttaaag aaactgaaga
    28021 agattcagat acagaaacaa aaacagaaaa agtatccgta gccgattatc gtaatcttga
    28081 agcggacgtt gcagcagctg atgctcaaaa aagtggactg caacctgtag tgatcggtaa
    28141 tggtaaaaaa gtacaaaaac aatctactgc caacggtgat cagttgatct ctggagaaaa
    28201 actgatccta tatactggtg gtgataagct gatgcctgac gtaacaggat ggtcaaaagc
    28261 agatatcatg aagctgggga aaatcctcgg tattgaagtt agttttgatg gagatggtta
    28321 ttgtgtcaaa caagaacttg ctccatatga aaaaatcact gaagacaaac ttagttttac
    28381 tttagaagaa taagaagaga aataggagag aaaagagcat ggaatggaca caagcattga
    28441 ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat gccgctattt attggttatt
    28501 ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg accaaagtgg cacaatagca
    28561 aagccggaac tcctacaatg ggcggcttag tgtttctgat cggctctatt ttgacaggaa
    28621 tttgggttgg agcttggcaa aaacagctaa ctcctactct tttcatcttg ctttttgtat
    28681 tagcgttata cggagtaatc ggctttcttg atgattttat aaaaatattc aagaaaagaa
    28741 acatgggact gaactcgaag cagaaattga ttggacagat tctaggggga atcatctttt
    28801 atcttgttta ccgttctgaa ggttatccag gtgtattgaa tttcttcgga ctagatctac
    28861 ctttaggaat catttatggt attttcgctc tcttctggct tgttggattt tcaaatgcag
    28921 taaatctgac agatggcatc gatggattag ttgctggctt aggaagtatc tcttttgcag
    28981 cttacggtct aattgcttgg catcagcatc agtatgatgt attaatcatt tgcttaagtg
    29041 ttttaggagg tttactgggt ttcttcgtct acaacagaaa acctgccaag atttttatgg
    29101 gagatgtcgg ttcattagcg ttgggcggac ttttagcggc aatttctata atgctaaacc
    29161 aagaatggac attgcttttg attggtttga tctatgttat ggaaacagca agtgtgatgc
    29221 ttcaagttac ttcctttaag cttacaggaa aacgaatttt taaaatgtct ccaattcatc
    29281 atcactttga aatgtgcgga tggtcggagt ggaagattga tacagttttt tggttgatca
    29341 gcattgtgac ttcgttgatc acattatgga tcgtttggta ggagatgacg ggaaatgaaa
    29401 aaaatatcaa catatgaaaa taagaaagtg cttgtcttag gactcgcaaa aagcggagta
    29461 agtgcggcca aattattaca tgaattgggc gcacttgtca ccgttaatga tggaaaacct
    29521 tttgacgaaa atccggaagc acaagagctg ctttctttag ggataaaagt aataacaggt
    29581 agtcatccaa tcgaattgtt ggatgaagaa ttttcattga tggtcaaaaa tccagggatt
    29641 ccctacagtc atcctttagt agcaaaagcc caagaaatgg gtattccggt aattacggaa
    29701 gttgagctag cttacgaagt agcggaatgc ccaattattg ggattacagg aacgaacgga
    29761 aaaacaacga caacaacgat gacaggacta ctactgaatg caggagcaga tcaaggaatt
    29821 gcacgtttag cgggtaatat tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca
    29881 gaagataaaa ttgtgatgga attgtctagc ttccaactca tgggaatcac agatttccgc
    29941 cctcatatag cggtgattac caatatctat gaagcacata ttgattatca tggtacaaga
    30001 aaagaatatg tcaaagctaa atggaacctt caaaaaaaca tgacagaaaa agattatctg
    30061 atccttaact ggaatcagtc agaactacaa gaattagcgc aaagaacaaa agcacgcgta
    30121 ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt atgctgacga ttactcaata
    30181 tactataaaa aagaaaaaat aatggaaata tctgaacttg gcgtaccagg taagcataat
    30241 gtagaaaatg cactagctgc tatttctgta gcaaaattgt atggtatatc aaatgaagct
    30301 atacgagaaa cgttgcattt tttccatggc gtaccacatc gtacgcaata tgtaggagaa
    30361 atccaaggca gaaaatttta taatgattca aaagcaacca atattttagc gactaaaatg
    30421 gctctaagtg gatttgaaac gtctaaggtc gtgttattgg ctggcggatt agacagagga
    30481 aatacatttg atgaattaat tccttcatta aaaggaatca aagcaatggt cgtatttggc
    30541 caaacaaaag aaaagttgat ggatgctgga aaaaaagcag gcatcgagac aatagtaaca
    30601 gccgattctg tagaacaagc agttccttta gcattagaaa acagtacaga tggtgatgta
    30661 gttttattat caccagcgaa tgccagctgg gatcaatacc caaattttga aactagagga
    30721 aacagattca tggaagctgt gaaccgatta aaatagaaaa agtagaagcg agcgataaaa
    30781 aatgaaaatt ttggttacag gcggaggaac agggggacat atttatccag ctcttgcctt
    30841 tgtcaattat gtaaagactc aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg
    30901 aggtctagaa aacaaaatcg tccctgatac aggaatgcct ttccatacct tggagattca
    30961 aggatttaaa cgaaaaattt ccatgcataa catcaagacg gtccgattgt ttcttaagag
    31021 tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca gatatcgtta tcggtactgg
    31081 aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa atgggtattc caacagttat
    31141 ccatgaacaa aacagtgtac caggtgttac caataaattt ttaagccgat atgtggatcg
    31201 gatcgctctt tcatttaaag atgcggcttc cttttttcct gaaaacaaag cagttcttgt
    31261 aggtaatcct cgagcacaag aagtagcaga tactaaaaaa tcagatattt tgaaaacatt
    31321 tgatttggat ccagaaaaga aaacagtctt gatttttggc ggtagtcaag gagcacttaa
    31381 aatcaatcaa gcagtatcag tttttttaag tgaatatatt ccttcttctt accaagtttt
    31441 gtatgcttca ggagatagat attatgaaga gatcctaaat aaaataaatc ctgaaataca
    31501 aaatattagt gtccagcctt atatcaaaaa tatggcagag gtcatggctt ccagtgatct
    31561 gctggtgggg cgtgctggtg caacttcgat cgcagaattc acagctctgg ggttaccggc
    31621 agtgttggtt cccagcccat atgttacgaa tgatcatcag actaaaaatg caatgagtct
    31681 tgttcacgca ggagcagcaa aaatgattgc agataatgaa ctaacaggtg aatcactaag
    31741 tcagacagtc aacgaaatca tgggtgatga agaattgcaa aagcaaatgt gccgagcttc
    31801 taaagaacaa ggaatccctg atgcttcaaa acgtctttat gacttagtaa aacaaataat
    31861 caaaaaataa aggcggtggt cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat
    31921 ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg ctaaccgaaa atatctggca
    31981 gaacaagaga tgaaaaacca agagccaaaa aaagaagaca taacattaga tacacccctt
    32041 tcagattctg aagaagaaaa aacggttcaa gaggaacagc aagaagaaag ccagtccgag
    32101 aaaaaaatcg aattttttga agagatcaag caaaagaaaa acggtggacc atataacggt
    32161 tcttttttga accggctgcc caatttaaaa agtcaacggc acaaagtact atttcgtcgg
    32221 ttagctttga tcatcactgt attgggtata ccgctgattt ttttgatcta ttatgtttct
    32281 ccttatagca aattacaagc agttgcagta tcaggcaata agacagtcaa cagccaagaa
    32341 atcatctctg atacaaaatt gtcattagga gaaaatgttt ggggacagta ttttcatcga
    32401 tctacttata tcgatcgcct taaaaaagca cagccaagaa tcgaaacagc aaatatacat
    32461 ttcaaaggga tgaatgaatt cgatttggat gtaacagaat ataaagagat tgctttgatc
    32521 gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa cagtattaga tgaaaaagta
    32581 gcaaatccta caaaaaatct ccctatcttg gaagagttca aagactctgc aaaaatcaaa
    32641 gaattaacaa agcaatacaa tcagttatca tcagaattac aaaaagcaat ttctgaaatc
    32701 aagtacacgc caagagcaag caataaaaat ctgatccaat tgaatatgaa tgacggcaat
    32761 caagttattg taaatatcaa taatcttgcg aaccaaatga aatattattc gcaagtagcc
    32821 aaagatatgg atgaaaaagg tgtgatcgac atggaagttg gtatcttctc gcatccttac
    32881 ggtagcactc aagaaacgga agaatcgggc caatcggaag aaacaactga aagtgaagaa
    32941 aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct
    33001 ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt caagtgaaac aagtcaaata
    33061 gaagataatc caagtacaac tccgtcaagt ttaaatgaaa attgataaaa caactttaaa
    33121 aagtaagaat gtactttcta aaaaccagta atttatggta gaattggaag cagtgaatat
    33181 atctattaat aagtatattt taaaacggac gctagttgtt cgcttgtata tggaatagta
    33241 agtaggaggg aatcccattc atggcaaaaa caggaatgta tgtaggcctt gatattggaa
    33301 cgacatctgt taaagttgtc gtggctgaat atatcgacag ccaaatgaat attattggag
    33361 taggaaacgc aaaatcagaa gggattaacc gaggcattat cgttgatatc gataaaacgg
    33421 tccaagcgat acaacgagca gtacgacaag cagaagaaaa agcaggtatc cagatcaaag
    33481 gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga aaattgccag ggaatgattg
    33541 cagttaacgg agattcaaag gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg
    33601 cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc aatattgcca caagatttta
    33661 ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat gatcggcgta agattagaaa
    33721 tgtacggtct gttgtttaca ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg
    33781 aaaatgccgg attaatcgtc aacgaaatgg tgattactcc tttagcgctg actgaatcaa
    33841 ttctttctga cggagaaaaa gattttggta cgatcgtcat tgatatgggt ggcggccaaa
    33901 caacaacggc tgttatgcat gataagcaat tgaagtttac caatcttgac caagaaggtg
    33961 gcgaatttgt cactaaagat atctctattg tattaaatac gtcatttaac aatgcagaag
    34021 cgttgaaaat aaactacggg gatgcatacc cagaacggac atcaccagat gaagaatttc
    34081 cagtagatgt gattggtcag tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa
    34141 ttatttcagc tcgaatggaa cagattttca acaaagcaaa agatgcattg gaccaaatcg
    34201 aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg
    34261 ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca
    34321 tggggctgag aaacccggta tttacaaatg taataagtat cgtggattat tcagcaaact
    34381 tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc
    34441 aaatggtagt tgaacaagaa acaacaaaca catatgagag ctatgaagca cctgaagaaa
    34501 caatctacga tgagccggaa ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg
    34561 gtttctttac aaatattttt gattaattca tcctgggagg aataagatat ggaattctca
    34621 attgataata atatcaatga cggcgcagta atcaaagtca tcggagtcgg tggtggcggt
    34681 ggaaatgctg taaaccgaat gattgaagaa aacgttaaag gtgttgaatt cattacagcc
    34741 aacacagacg tgcaagcatt aaaaaattca aaagctgaaa cagttatcca attaggcccc
    34801 aaatatactc gtggtttagg tgctggttct cagccagaag ttggacaaaa agcggcagaa
    34861 gaaagtgaac aatccttacg cgaagcttta gacggtgcag atatgatttt catcactgca
    34921 ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa
    34981 ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca cttttgaagg accgaaacgt
    35041 ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa atgtggatac attattaatt
    35101 atctcaaata accgtttatt agaagttgta gataaaaaga caccgatgct tgaagctttc
    35161 cgtgaagcag ataatgtttt acgacaaggt gtacaaggta tctctgattt gatcacagcg
    35221 ccaggttacg ttaaccttga ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca
    35281 gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa
    35341 gcaatctcct ctccattgct tgaaacatct attgatggag ctgaacaagt acttcttaac
    35401 atcacaggcg gtttggatat gacattgttc gaagcacaag atgcttcaga tatcgtagca
    35461 aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat ctatcaatga agaaatgggc
    35521 gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag
    35581 tctagccgac cagctagaca agcacaaatg caatcacctg ctcaaaagac tgtattagat
    35641 atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca gttttggcga ttgggatatc
    35701 agacgtgaac aaaatgttcg tcctagagtt gatgattcca attttgacaa tatcgaaaaa
    35761 aaagaatttg atacattcaa ccgcgaagaa acaaaatctc atggcgatga tgaattaaat
    35821 acgccaccgt ttttccgtcg taaaaaataa aggagaggaa gagcacatga ttgctgacaa
    35881 cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa
    35941 agaagttaca ttagtagctg taacaaaatc tgttgaatcc gctcaagctg aagaattggt
    36001 tagagctggc gttatgaatt gtgcagaaaa ccgagtagat aaattattag aaaagaaaca
    36061 agatttattt gattttcctg aaataaagtg gcatttaatt ggtaatttac agcgcagaaa
    36121 ggtaaaattg attgtaaatg aaattgatta ctttcatgcg ttggacagtt taagattagc
    36181 agaagaaatc cagaagcggt tagagaaaga attatcctgt tttattgaag taaatgtttc
    36241 tggtgaggaa tcaaaacatg gaatttcacc agatgagctt ttaccgtttg tggaatcttt
    36301 agctgcctgc acaaaaatca aggttgttgg gttgatgact atggcgccta aagaagcttc
    36361 tgatgagcaa atccataaag tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc
    36421 aaaaggttat gcccatgcac catgtagtga attaagtatg gggatgagtc aagactttca
    36481 aattgcgatt gaagaaggtg caacatttat ccgtgtagga acagcgctat tcaaagacga
    36541 ataaaggagg aacaaaaatg ccgcttttaa acagaggagc aattgctaat tttttcggat
    36601 tagctgatga ggaagagtac gaataccaag attatcctga aacaacaaaa aaggctgtac
    36661 agccaactaa aaaaaataca acacagaatc aatcaactca ttctgtatct gctgcgaata
    36721 ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac gcatgaaagt attcagccgg
    36781 ctgaaaagaa aaaagtaatt gaactacatc aatcacctac aaaaactgta gcgaaagaaa
    36841 gaacagcttc aagaacctct acacaaccta aagcaaatct tgctggaaaa atcactgtaa
    36901 tggaaccaag agcttattct gaagctatgg cgattgctaa acgaatcatt gctggagaag
    36961 caaccttagt gaattttcat ttagtagaag aaaaacaagc gagaagaatc gttgattttc
    37021 taacaggaac agtttatgca ctagatggag atatcaaacg tgttggagat gagatttttc
    37081 tttgtacacc gactgggatg gagattgaca gttcaaccgc tcaatctttt gcagatgcga
    37141 atttgtttga tctgtaagag gaggaactac gatttatact attgcactat tacttcattt
    37201 acttcaacgg gcagcctatt tctatagcat tatcttgatg gtatatgcat tgctctcttg
    37261 gtttccaggc gggtatcaat cagggctagg acgattatta gctaagatct gcgaaccgta
    37321 tatcagtttg ttcgatcgtc tgcctttgag gattggaccc ttggatttca caattttagg
    37381 ggcaatcctg atcttgaact tagccgtaca aggtttagca cagatcgtta tatggataac
    37441 tgtccattta gtttattgat tgaagcaaca aaatctttat tctcagaata gataggaggg
    37501 gaaaattttg gatgcaaatg tataccagca ctttcgggca gatgaacgta catttatcga
    37561 ttcggtaggc gattggattg aacaggttca aggacaatat gcaccctatc tgaccgaatt
    37621 tcttgaccca agacaatcct atattttaga aacactgatt cgacaaagca gtgatttgcg
    37681 tttcatgttt tatggcggat atgaacaggc agaaagaaaa agatgtttga ttttccccga
    37741 ttattacgaa cctgtagagg aagattttga aacagcattg tatgaaatcc attatcctag
    37801 caaatttgcc agcctaagtc atgggaaaat ccttggaact ttagtgggaa cggggatcaa
    37861 acgctcttac tttggcgata tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa
    37921 tgaggtggat cattttgttg ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt
    37981 ggaaccaatc aaatacacag aaatgattat gccgaaagat agctggacac aagaaagagg
    38041 aacagttagt tccatgcgac tagacagtgt gatctctgag atttataata tttccagaca
    38101 gcggtccaag cagctgatcg aagctggtaa agtaaaagtg aactggacag aaaatacaag
    38161 acctgatttt gtacttgaac tattagatat tatttcagtc agaggttatg gcagagtcca
    38221 gatccaagaa cttgagggga agacgaaaaa agaaaagtac agggtactgt tcggtgtatt
    38281 acgtaaataa aataaaaagg tggtttactc atggctttaa ctccattaga tatccaaaat
    38341 aagacattcc cgacaaaaat gcggggatat aatcaagacg aagtagatga tttcttagat
    38401 ttagttgttc gtgactatga ggaattgact caaagaaatc gagaattaga aaaagcagtc
    38461 aaacattctg aagaaaaact tgagtacttc aatgaattaa aagatgcttt gaaccaatca
    38521 atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa
    38581 gtgatcgtta cttctgcaca aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt
    38641 gcacatcaat taacaactga tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca
    38701 gaaaaagcaa gacagcttgc gacagaaact gaagatctta agaaaaaaac acgagtattc
    38761 catcaacgta tcagcttgat gcttgagtct caattagaac aagtgaaaag tccggaatgg
    38821 gatgaaattt tacaaccatt ctccagctac gtgactgaca gccatgaagt gatcaaagaa
    38881 gttttagcaa aacagcttga caatgaaaat gatactgatg taaactcaga cacaggagtc
    38941 atagaagcac ctatgacggc atttgataca caggctattg atatctcgat cgtgcctggt
    39001 gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt cttaaagcga gccgatgaga
    39061 gtggaagttc ggtaagacac tgcatggtga aagatcctct acttgagacc tgcttttccg
    39121 aaattagagt aagaagcagc ggctgacctc gttattggtc acagagagtt tgcactagat
    39181 agatgcaact aaatttgggt ggtaccacgg catgttcgtc ccttgcggct gaacatgctt
    39241 ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg
    39301 caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg
    39361 caaaaagaat gggaagaaaa tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa
    39421 cctagctttg ttcttcatga tggacctcca tatgccaatg gtaatatcca tttaggacat
    39481 tcattgaata aaatcagtaa agacattatc attcgttcaa aatcaatgtc tggctatcgt
    39541 gcaccatatg ttccaggatg ggatacacat ggattgccga ttgaacaagt attggctaat
    39601 aaaggcgtga aacgtaaaga aatgtccatg gcagaatact tggaaaaatg tcgtgaatat
    39661 gcactttccc aagtagataa gcaacgagca gatttcaaac gcctaggagt tgcaggagat
    39721 tgggaacatc cttatgttac attagatcct tcttatgaag ctgcacaagt acgcgtattt
    39781 ggtaaaatgg ctgaaaaagg ctatatttac aaaggattga aaccaattta ctggtctccg
    39841 tcaagtgagt cttctttagc ggaagctgaa attgaatata aagatgtaaa atctccatct
    39901 atttttgtag ctttcaatgt caaagacggc aaagatattc ttgatgaaga tacagctttt
    39961 gtcatttgga cgacaacacc atggacattg ccagcaaacc aaggtattgc tgtaaatcca
    40021 aaatttacgt atgtattggt ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg
    40081 ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa cagttctaaa agaatttgct
    40141 ggacaagaaa tggaatatat gactgcaagt catccattct atgaacgtga atcacttgtg
    40201 attttaggcg atcatgttac tttagacgct ggtactggat tagtccatac tgctccagga
    40261 catggggaag atgactatat cgctggtagc aaatataaac tatctgtagt gtcacctgta
    40321 gactcaaaag gattgtttac agaagaagca cctggatttg aaggtatctt ttatgacaaa
    40381 gcaaatccta tgatcactga tctattaaaa gaaaaaggag ctttgctgaa gctagatttc
    40441 tttactcata gttatcctca cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact
    40501 ccacaatggt ttgcatctat cgataaattc cgtcagaata ttttggatga agtagaaaaa
    40561 gtagagtggg ttatcccatg gggtaaaaca cgtttgtata atatgatccg tgaccgcggc
    40621 gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa
    40681 aatggagaag caatcatcac accagaaaca atcgaacatg tggcaaaatt atttgctgaa
    40741 catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc
    40801 cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag atatcatgga tgtctggttt
    40861 gattctggtt cttctcatga agctgtttta cgtcagcgac cagaattatc attcccagca
    40921 gatatgtatt tagaaggatc agaccaatat cgtggctggt tcaactcaag tatcacgact
    40981 agtgtggcta tcaatggtgt agcaccatac aaatcagtta tttctcaagg atttgcttta
    41041 gatggtgaag gccgtaagat gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg
    41101 atcaaacaat ttggtgcaga tatccttcgc ttatgggtat ctagtgtaga ttatgaagca
    41161 gatgttcgtg tttctatgga tatcttaagt caagtggcag aagtatatcg taaaatcaga
    41221 aatacgatgc gtttcttatt agcgaataca gaagatttcg atcctaaaaa agatactgtt
    41281 tcgtacaatg atttacgttc tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc
    41341 aaagaaatcc gagaagaagg gtacgacaaa tataacttca tgcatattta ccgtacagtt
    41401 atgaacttct tgacggttga tctatcgtca ttctacctgg attttgctaa agatgtagtt
    41461 tatattgaag cagaagatga ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca
    41521 gctgtagctt tgacaaaatt attgactcct atcattccgc atacagcaga agaaatctgg
    41581 tcattcctga aagaagaaga ggagtacgtt cagttaagcg agtttcctgg ttatgaagag
    41641 tttgcaaacc aagaagaatt gatggatacc tggactgctt tcatggactt ccgtgataat
    41701 gtcttaaaag cattggaaga agcaagaaac agcaaattga ttggtaaatc tttggaagca
    41761 aaactaactg tttatccaaa acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat
    41821 attgcacaat tattgatcgt ttcaccagac tactttgaag ttatggcagc agatgaacaa
    41881 gtgccagata acgctatggt atttgatgat gttgctatta cagtggaaaa agcagacggg
    41941 gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag gagtagatga aaaacttcct
    42001 catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa
    42061 ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat gttctttgtc acaccccctt
    42121 ttgttatcaa tcgtttaatt ttccacgttc acgataccag ttatctggtt gccagatcca
    42181 ttcgaatccg tctttttcaa gtagttcgaa agctgcttta ggtcccattg atccagcttt
    42241 gtagttggga aaatccggtt gtgtttggtc ccaagctttt cgaatgacat ccacgatatg
    42301 ccaagattga gcgacttctt cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc
    42361 taatagtaat ttttcgtaag cttctgggct attttcaacc atttctgcac tatttctaaa
    42421 ttccaatttg attggttccg tttcaaatcc ttgccctact tctttcccat tcaaggataa
    42481 agaaaatcct tcagttggct gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc
    42541 gtcacatggt tcatcgacag atgttttgaa tacattaaca ggaacttgtt tgaatacgat
    42601 gttgatccga gtcccttttt cagtcaaacg tttgcctgta cgtacataga aaggaacacc
    42661 tgaccaacgg aagttatcga tagtgaattt gcctgcgacg aatgtttcag tctctgattg
    42721 agcatccact ttgtcttctt cgcgatagcc cttgaattgt acaccatcca attcaccttg
    42781 agcatattgc ccgcgaacga agttttctaa cacttcttct tcgttataaa tgcggatagc
    42841 tttcaatgct ttgatctttt ctgtacggat ttctttttcg gaaaatgcta ctggcggttc
    42901 cattgcaagt aatgctacaa cctgtaaaat atgattttgc accatgtctt taagtgcgcc
    42961 actatgatcg tagtagcccc cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg
    43021 gatattgtcg atatagcgat tgttccattg agcctcaaaa atattattgg caaaacggat
    43081 agctgagatg ttttggatca tttcttttcc taaataatga tcaatcctga aaatatcttg
    43141 ttctgggaat acttctctga tttgctggtt caattcaaaa gcagactggt aatcagaacc
    43201 gaacggtttt tctatgatca aacgattgaa gccatttttt gtcataatat tttgtgattt
    43261 caaatgagaa acgatcgttc caaaaaattg aggtgccata gccaaataat agacacggtt
    43321 accgtttaat tgatagtgtc catctaatcg gtcagacaat tcttttaatg tctgataatg
    43381 cgttgaatca tttacatcat gggattgata gtaaaaatgg cttgcaaagg tagttgcttc
    43441 ttcatctgaa ggattcaatg cacggattgt ttctttgaca acttcacggt agtgctcatc
    43501 gctccaggga cggcgagcgg ttccgattac agcaaaatgc tcgcctaaat ttccttttct
    43561 gtaaaggcgg aacaaagacg ggtacaattt acgctgggct aggtcacctg taccgccgaa
    43621 tatcgtaaac aatacatttt tttcattaga catgtggata cactccttta tattttatcg
    43681 acaaaaatcg tgctggccgc cttaaagctg acggggattt tctttctatc attttggata
    43741 gtgatcggtc cttcataagc tgcgatgtca acgatctcat attcttcgtt gatattcagg
    43801 tcaatagtga ctaagtaatc caacaattct ttttcatcaa gcactcgtgc gatacgcact
    43861 tttgtaccga caggatagga ctccaatgtt tgtcggcgtt cttcatggac aacttcctct
    43921 tttttaggaa tcgatcctcc atgaggacaa tgttcaggat gattcaaata gttttctaaa
    43981 tgatctgcaa gatgttccga agtcacatgt tccaatactt cagcatcatc atggacttca
    44041 ttccagctat agtctaaatg ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc
    44101 agactgctag ctttttgcag tccgcttgca gtcaattgta caccttgata aggggaatgt
    44161 tctacaagct cttctttcac aagtttagaa atcatttcac tgacagatgc agctgagaca
    44221 tctagactag atacgatttg tttattgttg actttcgttg tatctccacc tagttctaaa
    44281 atgattttca aataatcttc tcgatttggt gtcatattcg atcatccttt catggtaaaa
    44341 aaccatcatt agtaagtgta acaaaaaaaa cgttaattga ctatttttct aaataaaaaa
    44401 caaaggattt ttagacgttt ttacaacaaa aaacgaaaga tgatagagaa gactcattct
    44461 ttcgtttata aaggatatat atatacattg ggagggatct atttttcaga taacaactta
    44521 tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg gataagaaaa gataagataa
    44581 aaaccatact gatcacgtac gactaaacga tattcatcta tttctaccac agatgtgcca
    44641 agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc
    44701 tggctatcac taaagaacca tgtaccttga tactttgaga gtccttctga cttttccgct
    44761 tgttttttct ttttcaagta aataaaactt gagagaacgg taatgagtga accaagaaaa
    44821 tagatcattt tttttatcac caaatcactc ctttatgaga aaataatcgc atagacatat
    44881 tctttcttct ttatttcatt gtaccgaata aaagctaaaa tttctctaag actttcgact
    44941 ggttttttta ctgtttttct acaaattatg ataggataca tagtaggaag aaaggtgaca
    45001 taatttgaag tatcagcaag tggaaatagc tttttttatt gaccgcccga atcggtttat
    45061 agcacattgt atgaaaaaag acggagaaat aattattgcg catgtcaaaa atacaggtag
    45121 aggaaaagag gtcctgatac caggcgctga agtggctatt gtatttgctc ctggacctaa
    45181 acggaaaacc gcctatgatc tgattgctgt aaaaaagaag ggtgactggt ttaatatcga
    45241 tagtcaattg cctaatcggc tggcaattga tggtatttta gacggcacga tccttttacc
    45301 aggattaact ggtccattta cggtatacaa gcgtgaagta acttatcttc actccaaatt
    45361 tgatatttat ttagagacgg tggtcggaca aaaagcattt gttgaagtca aaggtatgac
    45421 acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc acactcagag gattgaaaca
    45481 tataggagaa ttaacgtatg ccgcacaaga tggttatgcc gcttatgtct tgtttattgc
    45541 tcagttcgaa catcttcatc tggcaacgat ccatgaagaa atgcaaccag cactagctga
    45601 tatggttcga catgcgcagc aaagcggagt tcaaatcctc gcctataatt gtcaagtatc
    45661 ggcagatgag gtaactgtca agcaggccat tccatttgat gtagatatcc cttttgaaga
    45721 tcctatacaa tagaaagaag gtaaaataat gatcaactta ggtattatcg gaacaaattg
    45781 gattactcat caatttgtcc aagcagcttt aagcacaaaa aaatatgaat tgacagcggt
    45841 atattctaga catttggaaa aagcacagga atttggttct cattatgaag gggatatcga
    45901 attcgcaaca gatttgaaga cttttttcaa tattactcat atggacacca tttatattgc
    45961 ttctccgaat agtcttcact ttagtcaagc taagcaagca attttggcag gaaaaaatgt
    46021 gatagttgaa aagccagctt tttcgacgcc aaaagaaatg gatgaaatca tccatttggc
    46081 aaatgaacac aatgtgtatt tttttgaagc agcaagaaat atccatgaat caggttttaa
    46141 aaagattgcc gattacttac cattaaaaga cgagataatc ggtgcaaatt tcagctatat
    46201 gaaatattct tcgcgctatg accaagtatt agatggagaa gaaccgaata tcttctcgac
    46261 tcatttctca ggtggtgcat tgatggattt aggtgtttac cttgtatatg cagctgttgc
    46321 ttggtttgga atgccaaaag aagtccatta tttcccaaga aagatttcta ctggtgtaga
    46381 tggtcttgga tgggggatct tacgttatga gaattttgat gtatcgatcc agccaggtaa
    46441 aatcggtgat tcttatttac cgtcagagat ttattttgac tcaggaacgc tagtgatgaa
    46501 tggagtcaat gcaattgaac aagcacaatt tcatgatcgc gcgcataaag aaatcattga
    46561 tttggcggta acagctgcag aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt
    46621 catggctcat ccgaaagatc cagcttgggg attgttgtat gaagagtgga tcgagctagc
    46681 tagaaatgtg aaccaagtga tttatgattt aagaaaaaat ggcggcatca aattcgatgc
    46741 agataatgag gaagattttt aatgaacgaa tttgtacaag tatttcccga aaattggcag
    46801 aaaaaatatg aggataaagg gtttatctca ccttctctta ttcaacgagc agtttttcag
    46861 ccactaagta ataaacaaag tatagtagcg gtttctccta caggttcagg taaaacactg
    46921 gcttatcttt ggcctctact actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt
    46981 tttgcttctt ctcaagaact agctattcaa gtagcagatg ttgcacgtga atggggaaaa
    47041 gacaaagagt tgaaagtcca atctttggtg ggcggagcca acgtcaaacg tcagattgag
    47101 ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag gaagaatatt ggaattgatg
    47161 aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg tatttgatga agcagaccag
    47221 ctatttgacg ctgggaacag ccaaatcatt gatcaaattc ttcatcaagc tccaacagag
    47281 tatcagctag cttttttttc agcgacagca gatcgttccc ttgaaagcat tgaaaaaatc
    47341 actgggaaaa cgtttgagac aattgacgtg acagcagagg acgattctcg aaaaggactg
    47401 agacattact ttttacgtgt accgatacga aagaaagatg actacttgag acgtctcacg
    47461 catatagaag attttcatgg tctgattttc tttaatcaac tgactgaatt aggcgtaatg
    47521 gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc tggcctctga tcagaacaag
    47581 ctactccgaa aagcagcgct agaacaattc aaaaagaaaa tggtcagagc acttttgacc
    47641 acagatgtgg cggctagagg tttagatatt acaggtcttc cttatgtagt gaatgcagaa
    47701 gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa gaactggtcg aatgggtaat
    47761 gaaggaatag taattacact cgtacaagat aataatctaa gagatttaaa acggctttta
    47821 cgtccatcaa atataacact tgaagagatt tttttatacg gaggtaagct gcaaactgag
    47881 cagccaacca aagaagatgc agctcctgca gtaaataaaa agtatgcgaa aactttttct
    47941 ggaaaggagc agaacccaga atataacaaa gaaaaaataa gaaaaaaagt taaaaataaa
    48001 tcaaaaaaag agagaaataa aggagcaagg cgcaaataag cgccttttta tgctatgatt
    48061 atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta tgtgaaagaa cgtcaagaag
    48121 agatcttgca gctattgaca gaaaaccaac aaggattgac agcaagtgat gtagctgagc
    48181 gcttaactat cgatcgaagc aatgcaagcc gatatttaag tgaactatac aaggcccatc
    48241 atattgtaaa aaccgctggc cgaccagtcg tttactcttt gccaacggaa aagtcaaaat
    48301 ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt tgaaacactc gttggagaaa
    48361 atgattcttt aaaagtcagc atacagcagg caaaagctgc gattctttat ccaccgcggg
    48421 ggttgcatac gattattttt ggagaaactg gtacagggaa gtcgatgttt gctgaatgta
    48481 tgtatcattt tgcaatcgat tcagagatgc tttctgcaga tgcgccattt gtttctttca
    48541 attgtgcgga ttatgctcaa aatccgcaac tgctctttgg acacatcttt gggatcaaaa
    48601 aaggggctta cacaggagca gctcaagata gtcccggtct aatagccaaa gcagacggcg
    48661 gtatattgtt tttagatgag atccaccgtt taccgccgga agggcaggaa atgctgttta
    48721 ctttcattga taaaggtatt taccgtccgc ttggagaaag tgcacaagta catgaagcat
    48781 ctgttcagat cattggggcg acgactgaat cttcagaaag tttcttatct acgttcaatc
    48841 gacggattcc tatggcaatc accttgccga atcttgcttc acgttcactg gatgagcgtt
    48901 atgaaatcat ctctcttttt atcaaacaag aagcgaatcg tttgaatcaa cgtatcgata
    48961 tcgaaaaaga agcgattctt gccttcatgc tttatgatgc ggaaggaaat atcggtcaag
    49021 tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt acattaccgt actcatcatg
    49081 agaaaaagct aatcattcga aaagaagaac tttcccttca agtacaaaaa ggattactga
    49141 aaatcaagga aataccggaa cgtctcgacc gattcatgga cagcaaaagt cagtatttga
    49201 catttgaacc aggatttgct gatgttgtct ggtcacaaga tccagaacga aacatgcagg
    49261 tctataacga tattgaggaa aaagtcactt ctctttccga aaccggtgtg gagaacattg
    49321 atctagagac cctcatttca aaagatgtgg atgcttattt ccagacatat gtcaaagaat
    49381 tgactaaaag ttcgattccc aaagatcttt tgccagatga aatctggcgg ttaaccaatc
    49441 aattgtatga tgtggctgaa aaacggttag accgtattta taatgaaaaa gctcgttttg
    49501 cttttgcttt gcatctgcaa agtacattgg atcgtgtgag agaaggtcat atgatcgtcc
    49561 acccagattt gaataacgta cggaaaaatt ttaagaggga attccaggta gcaatcgatc
    49621 tttcaacgat cattgaagaa gaagaacaag ttgagattcc ttttgatgaa atcggcttta
    49681 ttagtatgtt tctttctatc aatctaggag aaagtgagcc tgttaaagaa aacaaagtag
    49741 atgttctcat attgatgcac ggcagacaga ctgcttcaag catgttggag atggcacaag
    49801 aactattagg aacaaaagta gggaaagctt ttaatatgcc actggttatg gaagttcaaa
    49861 caatgtatga agaagtgtta caatatgtca aaaactatca agagcatttg acgaatggcc
    49921 tactcttgtt aacagatatg ggatcattaa acaccttcag tgatttgatt tatgaagaga
    49981 ctggcattcg gacgaaagct atcacgatga caagtaccat gattgtgata gaagcgatac
    50041 ggatggcaag tgttggaaga agcttagaag atatttatca aaatatccag ctttcctttg
    50101 aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga aactttacag cgtaaaaaag
    50161 ccgtaatcgt gacatgcttt actggtgaag gagtagctgc caaattatat cagcgtatta
    50221 tgccagtgat taatcaaaat aaggtagaac tgatccagat gcagttttta gaacgtgaat
    50281 cattcaaaaa acacatcgat gccttattgg aagaatatga gatcaaagca attgcgggaa
    50341 cagttgatgt tgattatcaa aatatcccat ttttttcagc ctatgacgtt tttgatgatg
    50401 aaaaactaaa tattttaaag cgtatagctg atgatgagat cccgacagct aaaattgtcg
    50461 attcacttgc tggatcatta agcgatgtct tttctattga tgaactgatc cgttcattgc
    50521 aaaagacagt tcagcagatc cagaccgatt tgtcgattat cgtggaacct ggagtagata
    50581 ctggaatcgt tatccattta gccttcatgt tagacggatt gatcaaacaa gaaagccatc
    50641 ggcaatttga gaaactgtct attttctcaa aaatttatcg tttggaaatg gatatcgttc
    50701 gggcaaactt gatggcacta gaacgaaaat atcaggtaaa aattccagaa gatgatatcg
    50761 catttcttac acaaatgttt cttgaaaata aaataaaaaa atgaacacac taatcatact
    50821 gtttacacac taaacatctg tgtgcgcact ataaaaaaga gctaaaaagc atgaaaacgc
    50881 tatttcagct ctttttttta ttggcatgct tcttgcgtta tataaaagtg taaaaaaata
    50941 aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag tttgttcagc agggatgagt
    51001 acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag aaaaagggat ggaagccgat
    51061 atttttgcgg tctctgcctc agatgcagat aataatttag aatctaagaa tgtcgatgta
    51121 ttattattag gtcctcaagt acgtttcatg aaagctcaat ttgaacaaaa acttgcgcct
    51181 aaaggtattc cattagatgt catcaatatg caagactatg gcatgatgaa cggagaaaaa
    51241 gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt atgcagttgg gggtataaca
    51301 acgtggaaga tcaacaaaat ctagaagcca tcatggggtt aatcatgttt ggcggtaatg
    51361 caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat cggtgatttt gagttagcgg
    51421 ataggaaaat cgctgatgca gaagaatcac ttatacaagc gcatcatgca caaacagaca
    51481 tgttgacaca agaagcacaa ggaaatcata tgcaagtgac attattgact gttcacagtc
    51541 aagatcactt aatgacatct attgcgttta ctgatttagc aaaagaaatc atcgaacttt
    51601 atcgtcgcgt atacagcgaa taaatacaac ttcttttttg aacggaagaa gaaaaatttt
    51661 tgaacggatt ttttattttg ttataaggag aactctcttt atataaaaaa cttataggag
    51721 gaagtaaaaa atgaacggac tatcaagttg gatggagaaa tacatcctgc cggttgccgc
    51781 aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat gcttttatcg gcacactgcc
    51841 cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca attattcgtg atttaccgca
    51901 acagtttaac agtacctatg ctgacagttt agcagcagac aaaggaatct ttgccgtatt
    51961 gaatgtcatc atcggaatca atggttttgt ctggaacggt acactagcaa tcgccggatt
    52021 gatctttgcc ttttcttggg ggtataatct tgcccgagct tatggagtaa atgatctagc
    52081 tggggggatc gttggtttag ctaccttgat tcaaggtatt tcctttggtc cagcattatc
    52141 agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat gcaatcaata gttctgagat
    52201 tggtgcaacg attgcagatg gcacactgtc agttggatta tggggttggc taaaactaga
    52261 tcatttgaat ggaaacgctt attttacagt tatgatcatg ggagctattt cagtcattat
    52321 tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg cctgattcag taccaccagc
    52381 agtatctaaa gcatttgcag ccattttacc agcgacgatc gctttatacg tcattgctat
    52441 tatcaattat atcgtaggtc gtgtagctga tgggcaactg atcattgatt tggtgcaaaa
    52501 atatattgca gagccattcc taggattatc tcaagggttg ggagcagtat tgatcgtcac
    52561 agtatttgtt caagtattct ggttctttgg tattcatgga ccaaatgtat tggcaccagt
    52621 gctagaggga atctggggac aagcacaact aatcaatatt gatattttcc aaaaaggtta
    52681 taacggaaaa actggtacac cagccgtgtt agatgcaatc gaagatggaa aagcttatat
    52741 gtgggtccgc ggatcgttcg atgcttttgc atggtttggc ggatcaggcg gaacgatcgt
    52801 cttactgata gctatcttga ttttctctaa acgtgcagat tatctaacag tagctaaatt
    52861 atccattggg cctggtatct tcaatatcaa cgaaccagtg atgtttggtc tgccaatcgt
    52921 attgaatgca atcatgttta tcccattctt gattgctcca gtagtagcaa cagcaattgg
    52981 atggacagct acttatcttg gcctagtcgc tccggtatcg cagcaagtca catgggtagt
    53041 accgccgttc ttattgtcat tcttagcaac aggagcggat tggcgggcac cgatcgtaac
    53101 attagtatgt atggtcgtga ctttcttgat ttgggcacca tttgtcatct ctgcaaacaa
    53161 aatggaccct gccgcaggcg aacaacaata agatgaacgt gaggagcggg acgctttaag
    53221 tttcgttcct cataatgaat tttcgaggag gaactgaaat gattacaatt gccagtgcaa
    53281 tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa tgaagaagga atgactttta
    53341 catttaaaga aaaacaagga atcaagctgt tctttgaaac aagtgcagaa gatccggaag
    53401 ttgcggctaa aaaagcaaag gaactaatca aagcacagcc ttggggaaca gtcctttatt
    53461 ttcaatcaac agctgtttag aaaatggttt aaaaagatag gatggttgag atgataaaaa
    53521 acggtttatt tttatgtagt attggttctt tgatcgtgtt gtatagtttg aatccacatt
    53581 cgcaaaggta tgatttgtat agtatggtca caggtctttt tctaacagga ttaggaatct
    53641 atttcttcat gaaggggaaa aaaagagaag aagcaaagga gaaaaataac taatggaaat
    53701 cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat aaagttatgg actctgtcct
    53761 tttcgatgta cgtaaagcga ccggcaaatc agtaactaga aaacagctga aaaatttgga
    53821 gtacgtcaaa caattctcgc aaaataaccg ggctcgcata aaaatcgaag aagtcattga
    53881 aaaccagtct tataagtttc gtacatcgac aacgaagaat gaattcgtcg ttcattatca
    53941 aatcaaacca ttggacgatt cgacctgtga ggttcattac acagaaaaaa tggagtctta
    54001 tggcatgctt caaaagttga atgatatgct tttaggaaca atcttgatgt attttaaacg
    54061 gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg tattaaatca atgaaacatg
    54121 gtactattta gttattgaaa aatgaagtaa gctagaagga atttttcata ggaagataga
    54181 tctttcctgc gaatcctcac tagctttttt gtaggaaggg gcagcaaaat gacaaacaaa
    54241 gtaagagagc taggtgtatc ttcgattgag gaaatgtttg atttagccac ttatgcattt
    54301 aatggggcag atacacctga aaggcgagaa cgttttcgta cacttgcgga gaattcttgg
    54361 aattacggat tttttgatga ggaggatcgg ctaacaagtc aagtcatggc gacccctttt
    54421 ccagtgaatt tttacggaca agagtaccta atggccggca ttggctatgt tgcttcttat
    54481 ccagaagcaa ggggacaagg cgggatcaat cagatcatgg aaaagatcct tgaagattgc
    54541 cgtgatcgta aagtaagctt atcttatctg gcgccattct cctatccctt ctatcggcgc
    54601 tatggctatg aacaatcatt tgataaaatc agttatcaac taaacagtcg tgatgtgccg
    54661 tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt ttgaagatgc aaaagctgca
    54721 atcagaagaa tttatgaaca gatgccggaa aaccaacgag ctggtctgaa gcgcgaagac
    54781 tggtggtata cgtataaatt caagcaaaaa ggcaattacc agtttgcttt gcattttgat
    54841 gataatgagg aagcaacggg atatatcgtt tatcagcttg caacgtcttc ctttattatt
    54901 gcggagtggg gatttttgga tcatgatgct ttttgcagcc ttgttcgctt cgtaagttct
    54961 cacaatggtg cttttgaaac atttgaatat tcatgcggct acggcggtaa tgatcaaaat
    55021 tatctattag aaaatccatt tgcttctgta atgatcacac cttatatgat ggcgaggatc
    55081 gtagatattt ctttgttttt ggaaaattat ccatttaaaa agagacaagc tgcatttgcc
    55141 ctacaaatag aagaagatca ttatgcgaaa tggaatgaag gaatcgtgga agtaacaatc
    55201 gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa aactcccaat cgttcgagga
    55261 agtatccaac atatcacgca gctattatta gggtatcgta aagtggaaga gttggtattc
    55321 caagaaagaa tacaggtgac aaaagaatat gaagaaacgt taagctcaat cttgccacag
    55381 caacagccaa ttcttaatga ctatttttga taaaaaacgg gtgatgacaa tatttttgtc
    55441 accacccttt tgtttttcat ttggcatttg aaggaaaatg ttaaagtaaa tcctcttttt
    55501 tcgtaaattt gaattttttg ttttcttttg ttttagggtt ttcttttttg tggtcttgga
    55561 ttgcttcttt cgtaatcttt tcccgatttt ttttagatga atctaagcgt tctgcgtata
    55621 ctttttcttt tggcttatca ttcattggaa aggacctccc tacattattt cattctacct
    55681 ctagtttatc atctgttaag gactagtgga aacgttttgc ctaacttata agctgatttt
    55741 tttagcagat aagcttgcaa gtaaaaaaca aacaagctac aattgattta ttgaaattgt
    55801 tcctgtagct cagtaggata gagcaatcgc ctcctaagcg ataggtcgct ggttcaattc
    55861 cagtcaggaa cgtacaaata acccctgtat cctttgcggt acagggggtt attttgtata
    55921 agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc taagtatatt
    55981 tactccttga taaagtaaaa tagatgcatg cgtatgtctt agtccatcaa taaacagtcg
    56041 aagagaaaat agggaaaaat tgattactaa agaaaaactt actaggtcga cggtgtagtt
    56101 gattttcata cttcaataga ttcgaattaa acgatttcga ttggcttata ccagtcctat
    56161 ccaacgaaag atatatcgtt cttaactcta gcggtataac ttgaagcttt acgtaaagcc
    56221 aatgggaatg aactggagat gactttttat tatttttagt aatagctgat gggttaatac
    56281 ttacaacttt ccttttcaaa gtaccatata aaaaaacggt ggggttgttt agcgaaataa
    56341 aggatctaag ccaatagctt agatccttta ttctatgctt tatattattt tttgcatgtg
    56401 tgcgaaagta tactcaagcc caatcgccat ttcggaaaat cggtactgta gagccatctt
    56461 cacggattcc gtcgatatcc atcttgtcag atcctaccat gaaatctacg tgggtttgac
    56521 ttctattcaa tccagcttct gctaattctt catcggacat ttctgtaccg cctttcacac
    56581 tgaaggcata agcagaacct agtgctaagt gattcgatgc gttttcatca aataatgtat
    56641 tgaaaaagat gatgccagat tgagaaatag gtgaagggtc aggtacaagt gcgacttctc
    56701 ctaaacgacg cgcaccttca tctgtatcaa gcagttttgc taagacatct tctccttgtt
    56761 cggcagaaaa atcaacaact tttccatctt taaaagtaaa tttcataccc gagatgatgg
    56821 tgccagcata actcagtggc tttgtacttg aaatgtaacc atctactcga tgactgtcag
    56881 gtgctgtgaa cacttcttcc gtcggcatat ttgccatgaa tttttctcca cgagcattat
    56941 agcttccagc gccttcccaa agatggtttt taggcaatcc gataatgata tctgtaccgg
    57001 gagcagtata gtgaagggca gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta
    57061 atttttcatc atgcttcttc catgctaaga cagggtcttc ttcatagaca cgagtcgttt
    57121 tgaaaatctg atcccataat gcctcaactt gtttttcttc tggcagttct gggaagactt
    57181 ttgctgccca ttgtttacca gccgcagcca cgacagtcca actgacttta tttgcttgag
    57241 ttgccttgcg taaattcatt agtgctttgc cattcgctga ttgataagac gctactcgat
    57301 ggctatctac accagcaaat gcatcgggat cggcagaaac aacgctgatc ctgcttgctc
    57361 ctttttcaag ccattcatcc gcttgatcga ttttagattg tggtacattc tcaatacgat
    57421 ccgttgcagc atgtaaaaga aattctcttt ggatctggtc gtcagtccat tgaacgataa
    57481 cctcagcggc tcctagttta taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt
    57541 ccacgctgat ctgtaaaaca actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa
    57601 ttaagcgtgc atattttttt agaagttcat taaaatcaga taacataata aaaattcctc
    57661 ctcaccattt tttctggtaa ctattagcat gataccattt aattatgatt caaacaatga
    57721 ggctttaaat aaaaaaagga ggttattttt ttaaggttaa gtttcatgat atatgttact
    57781 acttgctatt tcagcttgtg aagaaaatca aaaactaatt ttgcatatca aaaaaacatg
    57841 ttacaatgaa attaatcaat ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata
    57901 tggcaagaaa aaagacgatt actagagaac agattttggc agctgcttat gaagtagctg
    57961 ccaccgatgg atttacacga tttactgcga gaaacatcgc gaacaaaatg aattgttcca
    58021 cgcaaccgat ttatcttgag tttaaaaata tggatgactt gagacaagct ctttttgagc
    58081 agatctatga tcatttacaa tatgaagtgt tcactgttga gcatacaggg aatacgatta
    58141 tcgatcttgc ttttaattat atccattttg caagaagaga gaagaagttg tatcgttcac
    58201 tgtacttaga agaatatggt ggaggaaaag agatgcagaa tttctcttat acttactttg
    58261 tgaatacagt gaaacgcgat ccagcttatg tgaatctttc agaagagcag atcgaatctt
    58321 tgtataatgg gacatggatc atagctactg ggatcgcagc tttgatgtct tcaagcatca
    58381 tccatccaac tgatgaacag attgaaaaga tgatccaaga cagtatagat gctatcttga
    58441 aattcaaaga accaatcgaa atcgattgaa tatactcaaa agttccacaa atcagtaggt
    58501 aacaagagat gtgtcaaaat aaggaaaagt tcgtttttct tacagcgtat gcatttttaa
    58561 ctcccgaaga actcttccat gaatgcaatg aataaaagag ccatacagat tgaataaatc
    58621 tggatggctc ttttattgca agtatattaa atttttgcga ctagttcttt agcgaaagct
    58681 tctaaatttt cgatatcttc ttcttcagca ttcagatcca ctttaacatt ttcggctcct
    58741 tttgatgcgc ctgtttttgc aaaagcagca tcgaaatcat ccaccgattt gcagaattca
    58801 tcgtaaaaag tatcaccaga tccgcagaca ccgtaaattt ttcccaatag atccaattct
    58861 tgcaagtctt cataaaagtc tacgatctca tcaggcagat cgccatcatc gtatgtataa
    58921 gtagctacta cgcaaatatc tgcctcctcg aattcatctg catctacttg ggtacattcg
    58981 ttgatttcga cttcgatatc cagattttca aatgcttctg caacaatatc ggcgatttct
    59041 tcagtgtttc ctgtcatgct agcatatacg atttttgcta aagccatgat gtgcctcctc
    59101 gtttataatt atttaacaat tatgaattat accaaaacat cattgaaaag aaaataaaac
    59161 ctaaagacac ctggggaatg tcatttggct aaatgtatgc cgctatgtta aaatgagagg
    59221 gatttgtaaa aaggagacga aaatatgatt actttaaaat cagcacgtga aattgaaatg
    59281 atggcagaat ctggagcttt attagcagat gtacataaaa atctgcgtga ttttataaaa
    59341 ccaggaatca caagttggga tattgaggta tttgtcagaa actatatcga gagtcatggc
    59401 ggaattgctg cgcaaattgg ctttgaaggg tatgaatatg cgacttgtat cagtataaat
    59461 gatgaaattt gtcatggatt ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa
    59521 gtcgatatgt gtatcgattt gaaaggcggc atttctgatt cttgttgggc ctatgtagtt
    59581 ggagaatcta cacctgagat cgaccgattg atggaagtaa cgaagaaagc tttgtacatc
    59641 ggtattgagc aagcacaagt agggaatcgg attggtgaca tcggtcatgc tatccaaact
    59701 tatgtagaag gcgaaaatct agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct
    59761 acgattcatg aagaaccagc agttcctcat tacggagaag ctggaaaagg gttgcggttg
    59821 aaagaaggca tggttatcac gatcgaacct atggttaata ccggaacttg gaaaatgaaa
    59881 atggatccta acggctggac tgcgtataca agagacggcg gactttcttg ccaatatgaa
    59941 catacattgg ctattactaa agatggtcca agaattttga catcacaagg agaagaaggc
    60001 acttattgat ctatccagtc aataggtacg ggagaggtac atgaaagttt ggaacaaaat
    60061 aaaacaaaat gaaaatctga tgcgctttat tgagacgact caaaagcgca tcactgattc
    60121 agaaatgagt acgacctctg tcgtcgttgc ttattattta ctcttgtctt tatttccatt
    60181 gattattgct tttggtaata ttttaccgtt tctccacatc gatcaagaga ctgtactgac
    60241 ctatttacga gaagtgattc ctgagaccgt ttaccagttt atcggtcctg caatcaaaga
    60301 ccttttgaca caaagttcag gaggcttgtt atccgtttct gcattagcgg ctttatggtc
    60361 tgcaagtcaa agtatcaatg ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa
    60421 tagaaaaaat tttttgatcg tccgtttctt ctcactaatt gtcattgtct tgttcatgat
    60481 tgctattagt ggggtaacta tcgttcttgg gttgggacag ctgattttgg atgctattca
    60541 gccaatcgta caaatacccg tgaactttat cgatcagttc caaactttga agtggccaat
    60601 cacattggta gctttatttg tcatcatgtt cttgatttat ttaattgtcc ctaatgctca
    60661 gttgaagttg aagacgatca ttccgggcac gatcttcgct actgtcggct ggatgctgtt
    60721 atcacaagtg tttagtatct atgctaggta ttttgcaacg aggatcagcg gttatcagat
    60781 cataggaagt tttattgtac tcatgctttg gcttaatttt gctgctacga tcatcatttt
    60841 gggcggaatc atcaatgcag ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg
    60901 tcgagatcca agccgaaaat ggttcaataa attgaaaact aaattttctc ataaaaaaaa
    60961 gtagaaacaa gagttcttat tgtaaattta tatcagtaat ttataataat tatgactctt
    61021 aattaaagta ggtgcccata tgcttgtttc ttttagtatg gttataagat tttttctgac
    61081 aattttatta tcactgatat taacacctgt attcaaaatc atttcagtac aaacaggtat
    61141 ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca atgccttcag cgggcggact
    61201 gcctatcttt atttcatttg ttatatcgac tttattctta ttccgtaaaa ttatccctca
    61261 atcgtatatt gtaccgatct tgattgcttc tttagtaatc atagtcacag gagtgctcga
    61321 tgataaatat gagctgacac ctaaacaaaa aagtataggg atcttgattg cttctcttat
    61381 tatttatttt gtagcagata tacgtattga ttccttcacg cttcctttta ttggatatat
    61441 ccaactaggc tggcttagtt ttcccgtaac gattttttgg atcttcggta tcacgaatgc
    61501 ggtcaatctg attgacggtt tggatgggct ggcggcaggg atttcattga tcggtttaat
    61561 tacgatagga atcattggat attttttcct ccatgcttct acagtgtata ttccagttgt
    61621 tattttctgt cttgtagcaa gtattatcgg tttttttcct tacaattttt atccggcaaa
    61681 gatttattta ggcgatacag gagctctatt cctagggttt atgatggccg tcctttcact
    61741 acaagggctg aagaacgtga cttttgtttc atcgatttct ttgctgatcg tcatgggagt
    61801 gccggtaaca gacacgtttt ttgctatcat taggagaaaa gcaaaccgtg tttctttttc
    61861 tacagcagat aaaaaacatc ttcatcaccg actaatcagt ttaggcttta ctcataaagg
    61921 tgctgtcctg acgatttatg cgatggcttt gatgttttct ttcacagcta tggtcatgaa
    61981 ttatactgga agaatcggaa cgatcgtatt gattattgcc atgttgtttg ctgcaatttt
    62041 attatttgaa ttgattggtc tgatcaatga gaaacatcag tttattttaa aaacattgcg
    62101 atttttaggg aataaagagt accgtacgca agtaaaacag aaatatttga agaaatggaa
    62161 ccgtcatata gatgaataac aaattaaacc agccgatcct tattagtatt gtgacggcaa
    62221 acagtgataa aatttttcgg acactagata attttatttc aactgtaaaa gatgaaacag
    62281 cagtaaaaat ccgaattttt gacaacaatt cacttcccga gtacaaaaaa agactaacag
    62341 agtataggaa atatccctac atcgatatct attttcatga tgaaaattcc ggatttggtt
    62401 atggacataa tcacaatctg atagctagcg attctcgcta tggcattatt tgtaacccag
    62461 atattttagt ggatgaagag acagttacag cattggtctt gttattgaaa gaacatccgg
    62521 aatgtgcgat gttagcacct aagattctta atgaagatgg aacaactcag cacctcatcc
    62581 gtgagcagct ggctgttttt gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa
    62641 aaaagtttga caaacgactg gctgtgtttg aatgccgagg attgcctact gatgctaata
    62701 gttatgtacg gatgatctca ggtagtttta tggtagctga tattgataaa tttaaagaga
    62761 taaaggggtt tgatgagcgt ttcttcatgt actttgaaga caacgacttg tgtttgagtt
    62821 ttgaaaaagc aggttacaag ttattgtaca caccctttta tgcagttatc catttttacg
    62881 ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat
    62941 ttttcaataa gtgggggtgg cgttttttct gatgaaacca gaaatattag tagtcgttgt
    63001 gctttatcag caactattca gtcaatctcc agcttatgga ccattaagca aagcacttag
    63061 cgaaaagcaa attcagctta ttatttacga taatagtcct caaaggcagc aaaatgagct
    63121 gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa aatcctggtt tagcggtggc
    63181 ttataatttt gcgttgtctc aagcagatga aggaacacgt tgtctcgtca ctctggatca
    63241 ggatacgagg ctagtcgacg attattttga actattgcgt aaagtaacct ttacagatga
    63301 atgtgttgct gcagttccga tgattttttc tgggagccga caagtttctc ccctatattc
    63361 agatcgttat atcaatcgta cagctcaagc agtagaagtt gaaggcccaa cagctgaacg
    63421 aatcatggca atcaattcag gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg
    63481 gtttaatcct gctttttctt tagatttttt ggatcattgg ctgttttgga aagtggatca
    63541 gttggaaaaa actgtggaga tccttcctgt acgaatggaa catgatcttt cggttttaga
    63601 ctatgaaaaa gtttcaagta atcgataccg ttctatttta caagcagaaa atcgatttta
    63661 tcatgaatat gatcaaaggc atttatccag tcaccgacga caattattgt taagaacagc
    63721 taaacagttc ttgaccgtca aaaaccggca tatctggcgg atgactttgc gctcatatgt
    63781 taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat agctaccttt aatggaggca
    63841 gatatttaag agagcagtta gatagtattt tatctcagct ttcagcagaa gatgaagtag
    63901 tcatttctga tgatgggtca gtagatgata catgggatat attaactgaa tacgcagaaa
    63961 aagataagcg aatccagctt ttttcggggc ctcgtcaagg attgatcgct aattttggtt
    64021 atgcgatcca gcagacgaaa ggagatctaa tctttttggc agaccaagat gatgtttggt
    64081 tggagacaaa agtgaaaaag atcactgact attttgagca gaatccggat cagctagtag
    64141 tagtttctga tttaatcatt gtggacagcg agctgcaaga gatccatgat tcgtattttt
    64201 cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat aagaagtcac tatattggtg
    64261 ctggtatggc tttccggtct tctttaaaac ccgatatttt gccgatcccg tcagaagtgc
    64321 cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa gactgggttc ctaagagaac
    64381 cgttaactct gtaccgaaga catgatttaa acgcgagtga gattgaaaca cattctaaat
    64441 tacggcaaaa aatttcttgg cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt
    64501 ttaaacgttg ataaaagatg tttaaatcct taatatttcc atgacaaata taacaattga
    64561 tgagataata acaaagtgaa atttgaaatc aaaaaggaga gcagatatcc atgaagggaa
    64621 tcattcttgc tgggggcagc ggaacacgtc tgtacccttt aacaaaggca acatcgaaac
    64681 aattgatgcc gatttatgac aaaccaatga tttattatcc aatgtctact ctgatgttgg
    64741 cgggaatcaa agaaattctg attatttcaa cgccacaaga tacaccacgt ttcaaggaat
    64801 tatttggaaa cggaaatgat ttaggactgc agatcgaata tgcagtacaa gaaagtccag
    64861 atggattggc acaagcgttt atcattggag aagaatttat tggagatgat agtgtctgct
    64921 tggtattagg ggacaatatt tactatggtg gcggtctgtc taaaatgttg caacgagcag
    64981 cttcaaaaga tacaggcgcg acagtatttg gctatcatgt caatgatcca gaacgctttg
    65041 gtgtagtaga atttgatgag aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc
    65101 caaaatcaaa ttacgctgta actggccttt atttttatga caatgaggta atttctatcg
    65161 ctaaaggtat caaaccttca gaacgtggag aactagagat tacggatgtc aataaagcat
    65221 atttggataa aggaaaacta tccgtagaac ttatgggacg cggatttgct tggcttgata
    65281 caggaacaca tgaatcattg cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc
    65341 agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg tatgggctac atcgataagg
    65401 aacaattgct ggcccttgca cagccattga aaaaaaatca atacggtcaa tatttattga
    65461 atttggccgc tgaataagga gtccttacag atgaaagtaa cagatacaaa attaacagat
    65521 gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg gtttcttcac agaaagttat
    65581 tcaaaagcaa aatttgcaga acatggattg aaatatgact ttgttcaaga caaccattca
    65641 ctatcagctg aagctggtgt ccttcggggg cttcatttcc aaaaaggaga agctgcacag
    65701 acaaaattga ttcgggtagt cactggagca gttttagatg tagtagtgga tatccgcaaa
    65761 ggtagcccga cttatggtca atgggacgga tttatcttat ctgaacataa tcatcgacag
    65821 ttgcttgtac ctaaaggata tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc
    65881 ctttataagt gtgataatta ctataatgca gaagcagatg ggggcatcgc atttgatgat
    65941 ccagatttag cgattgattg gccaattgat ccatcaagag cgattatgtc ggaaaaagat
    66001 caccgtcatc cgacattaaa agaatttgaa gcagaaaatc catttgttta cggagaaatc
    66061 taagaattaa ggagaaattt tcttatgaaa aacatcattg ttaccggagg tgccggattc
    66121 atcggctcta atttcgttca ttacgtggta aacaatcatc cagaagtgca tgtcactgtt
    66181 ttggataaac tgacttatgc aggaaacaaa gagaacttgg ccggacttcc tagcgatcgc
    66241 gtagaattgg tcgtaggaga tgttgcagat gcagaattag tggaccgtct agtaaaagaa
    66301 gcggacgcag ttgttcatta cgcggcagaa tcccataacg ataattcatt aaaagatcct
    66361 ttcccatttg tacaaacgaa tatcattggt acgtatacac ttttagaagc ttgccgcaag
    66421 tatgatgtac gctatcatca cgtatctact gacgaggttt acggcgatct tccattacgt
    66481 gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat
    66541 aatccttcaa gtccatattc ttctacaaaa gctggttcag atctgttagt aaaagcatgg
    66601 gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt caaacaacta tggtccttat
    66661 cagcatattg aaaaatttat tcctcgccaa gtaacgagta tcttaagcgg tattcgtcca
    66721 aaattatatg gcgaaggaaa gaatgttcgt gactggatcc atacgaatga tcattcttca
    66781 gcagtgtggc tgattttgac gaaaggcaga attggcgaaa cctatctgat cggtgcagac
    66841 ggtgaagaag ataataagac cgtgatggaa ttgatcttag aaatgatggg ccaaccaaaa
    66901 gacgcatacg accatgtaaa cgaccgcgca gggcacgatt tgcgttatgc aatcgattcg
    66961 acaaaattgc gagaagagct aggttggcag cctgagttta cgaacttccg tgatgggttg
    67021 gctgacacga tcaaatggta cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc
    67081 gttgaagcag catatgccaa aaatggacaa taatacagag gaaatttcac tgtattaatg
    67141 gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt taaaataaaa atcaaggagc
    67201 ggataaccat atgtttttat taactggagg aaatggacaa ttaggtacag aattgcgcca
    67261 tttattagat gaaaaaaatc tgccatatgt ttcaacagat gcaaaagact tggatatcac
    67321 agatgctgag aaaacaatgg cttatattac agaattaaaa cccgatgtta ttttccattg
    67381 cgcagcttac acagcagtag ataaagcaga agaagaagca aaagaattag atgagaaaat
    67441 caatgtcgat ggcacaagaa atgtagcact tgcagcaaaa gctgtgaatg cacaattcgt
    67501 ctatatcagt acagactatg ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga
    67561 cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt
    67621 agaagagatt ttagaagact attatatcat acgaacttct tgggttttcg ggatctatgg
    67681 acacaatttc gtcttcacga tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt
    67741 aaatgaccag tttgggcgac caacttggac acgtactttg gcagaattta tggtatatgt
    67801 catcgaagaa aaagcaccat ttggtattta tcatttgtcg aacgaaaata gctgttcttg
    67861 gtatgagttc gcaaaagaaa tattgaaaga tacagatgta gaagttgcac ctgttacgtc
    67921 agaagaattt ccgcaaaaag caacacgtcc gcagtattca gtcatgagcc tgaaaaagac
    67981 agaagcgtta ggttttgtca taccaacttg gcaagaagca cttgcgcaaa tgctggaagc
    68041 tgccaaaaaa atgaataagt aatttccttt ttatcaaata gaatttggag catgatatgg
    68101 aaaaacaaac aagaagtgca cgacgaaaag aaaagccaaa agcgaatcag gcagattcga
    68161 atttggtgct tgacctgttt ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg
    68221 tcatcgttac ttttaattta aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc
    68281 aaagagacga tgggaaaaaa actgatgtga tttttggaca gaatcgggaa gtgcggtcag
    68341 acgaatggct ggtgcagaca cctttttacc tctcacaagc tgaagaggac ttttcgttgg
    68401 tgaacgagga ttattcatta aacggacaaa acatgatcat tgcgtataat tctcctgtaa
    68461 aggatattac ggttatcgga aaaccattca actggggatt ctttttcctc ggaagagatc
    68521 gaggactttc cttttattgg gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag
    68581 tactgatgat attaacgaag aggaaaaaag ctctatcgct aatcggtgca tttgcgctga
    68641 cattttctcc agcagtccag tggtggttca tgcaacatgt gggcgatttg attttcttta
    68701 ctttgggact gatgattgct ttttatcatt atttctacaa tcatgataaa aaatggctac
    68761 gaacgttgat gatgcttttg gtggttattt tcggtattgg gtttatttta gtgatttacc
    68821 cagcccatca agtgatgctg gcgtacttgc tgatttttta ttttgttggc ctgcttattt
    68881 actatagcaa gaaagtttca tgggatctgt tggatgtagt gttgattgga ggggcagttt
    68941 tatttattgg cggagtcatg gttcatttct ggctaacgtc aaaagacgca ttgttagctt
    69001 ctttgaatac cttatatcca ggaaaacggg taagtactgg aggaaagtgg acgattggcg
    69061 aatttttttc tttccttaca aattggaaaa ttccttttga agatatcaac tattcgaata
    69121 actctgaagt ggccctgttc tatcactttt ttccaactgt attcctagca agtccatttg
    69181 tgttatttgg caaaaaagat agcgaacaaa agttactggg acgtattttg atgatttttt
    69241 gtctgttttc tatcttttgg attaccgttg ggttgccaaa atggctagca gaaatcactc
    69301 tgctgtctta tgttcctcca ttaagagcca tccttacttt tagttttgcg gcttgtttat
    69361 tatcgatctg gtttatcgct tatatttgga gagaagatgt tattcctatt tggtataaat
    69421 tcattttgtt ggcagtaaat gcatggtctt atttctatgc gattacgcat tcgaaaatgg
    69481 ataattattt ttcggatttt gaaaccatcg cagttgctgt gttgggaaca gtgattttag
    69541 cttttgcatt atttggctgg agacgcttgt tttacctcgc ttttgtcgcc ttagttatcg
    69601 tatctggctt tttcgtcaat ccagtcgtag aaggtacagg agcgattttt gaaaaaacac
    69661 tggcaaaaga aatccaaaaa atcgatcaac aagatcctgg taaagtttgg ttgtctgaag
    69721 atgaattata taacttcacc ccaacattgg gagtgaaatc cttcaataca gtacgctttt
    69781 atccagatat agctgcatgg aagaaaatcg atccgaaaga agaatacgag aagtattata
    69841 atcggtatgc tcatacaaga gcttttatcg ctaaggaaga tacaaagttc attttagatc
    69901 gtccagatat gttttcggtc acgttgaatt ttgaagacgc agaaaaatta gggatcgact
    69961 atgtgatcag taaacgacca ttaactgatt acaaccaatt atatgaagcg cagttcacgc
    70021 agctatatgg accggataaa gacggttata tgatttttaa agtagaatat ccggattatc
    70081 cagatgtacc acaaacacaa actcaggaaa atcagctagt cgtacagtaa taggctagct
    70141 gagaataatc ctgaaatatt agtagaaagg agagtgaaga taattcaagc aatcgtacaa
    70201 aaatttgtca acaaaatgag tgtcggtttc ttgcttttgt tgctgacgct agtctttttt
    70261 gtttggacag caggggtagg ttttccagca actttgttta tatataacaa tagttcatta
    70321 cttatcgctt ttagtgtggc tattttgctc attttaaatg tttataaagc aaaaggcgtc
    70381 gattttcttt atctaggtgt tgcgcttgcc cttttcattt tcttttctta ctttcgggaa
    70441 ttaagaaatg agtctttatt tggagactta ctgtttcctt tggttatttt attttgttta
    70501 gtaattaaat ggatcgagtg gacaaaatgg gatcgcggta tttatctgat tgtctttacg
    70561 gtcttcctcg gaatcacgtt atatcgtgta tttacagaga tccaagtacc agaaggacaa
    70621 agcatctggg ctccaggcaa ttcgctttct gatatttgga tcaatacgaa tacaatcgga
    70681 agttctttga tgactcttgc tttattgatc agtggatttg ccagttcttt tgaacgatgg
    70741 tatgttcgaa tcatcggtgg gatagcagtc gttgcagcct ttttaggtat ttgggtctgc
    70801 caatctagag gtgcattagt cgcgttgatt gtttttgcga tattggatct tttacctaaa
    70861 tcgttgatgc gtgtcattcg tgcgccattt attgcctaca cggttacgat tcttttagct
    70921 ttgccgattt cttatctggc ggctgtctct gaaaaagtca atttgtttac cgatcgagaa
    70981 gatatttggc ataaattcta tcagacgcta ggagaaaaat ctgaacaagt tttactagga
    71041 atgaaaacat ttatcttcca gcgaggaaac caatttttag gaaaccacaa tagttataat
    71101 tctattttga atatctatgg attgatagga tttgggattg ctgctttgct gcttatcctt
    71161 tttattggtc gcttaacgtt gaaagcagat ttatccaacg ggcagatgac ttttatctgg
    71221 gccttttttg cggtgatgat gcagtcattt atggaggata cattgacttc atacccatgg
    71281 gttccgatta tttatatcct gttggctatg gccgttcatc gatatgatga accagaagtt
    71341 gcggaggaat tctatgttat ggaagaggca tatgacgata tgacttatga tgatacatct
    71401 tatgaggaac aaccagatag cgctcattct tccaggatcc aacgctatca ttgataggca
    71461 caaaatacga gagaggctgt gacaaccgtc gcagcctttt ttggtttatt gcaattgtct
    71521 agcatcttct gtaaaataga aacgttagag aaaatcttgt atcatagaaa attaagaaag
    71581 aaataagaac gcagctcggt accaaaaata tggtaaaatg gcacttgact gtaattcact
    71641 gaaaatcgct tggaggaaca caaatgttta aagatttgta tttatttatg aaggacattt
    71701 atcagaacag aaaattattg ctacagtttt cgatcaatga cttcaaatca agatatgcag
    71761 gatcgttttt aggaatctta tgggcgttta tcaatccgct atttactgta ttgatttatt
    71821 ggctggtatt tggtgttgga ctgaaagcac aaatgatcga tgcaaaatac ccatttatcg
    71881 tatatttagt taccgggatg attccctggt tcttcttctc agatgcattt gcatcaacta
    71941 cggttgtttt tcgggaatat acttatttag taaagaaagt cgtattcaat attcgtatct
    72001 tgcccacgac gaaaatccta tccaatctgt acacccattt attcttcatt ctgattggca
    72061 tagctgtcgc gcttataaat ggtatatatc cttcagcaat gtctttacag ctgatttatt
    72121 atttattttg catgtgtgct ttcttgacag gattgacttg gctgacggca tcgatccaac
    72181 catttttacc tgatatcatg caattaatta cgatcgtgct gcagttgatc atgtggacat
    72241 taccgatatt atggagccct aatcagtttg attcaggtct tgtaagcata ttgaaattaa
    72301 atccgctgta ctatgttgtt caaggttatc gtgagtcatt tttaagtgaa gcttggttct
    72361 gggagcatgg aacatatact ttgtatttct gggttttcac gttagtaatg ttcgtaatcg
    72421 gctctgcagt gttccgtaga ttgaaaccac atttttcaga tgtgctgtaa ggagaatagc
    72481 tatgacagaa tatgcaatta gactaaaaga tatcacaaaa tcttataata tgtacgcaaa
    72541 accttcggat cgatttcgtg aagccctcaa tccttttaag aaatcctatc atgatgtatt
    72601 ttatgcgttg aaaaacgtga atgtagaaat aaaaaaaggt gagatgatcg gtttcgtggg
    72661 agaaaacgga tcgggaaaat ctacaatgtt gaaaatcatt acgggagtac tgacaccaac
    72721 atccggcacg atggaaatcg aaggaaaaat ctcagctttg ctagaactag gatcaggatt
    72781 caatcctgag tattctggct atgaaaatat ctatttaaat ggaatggttc taggattttc
    72841 aagagaagaa gtcgatgaaa tggtcgatga tatcattgaa tttgctgaca tcggagacca
    72901 tatttatcaa cctgtaaaaa cttattcaag cgggatgttc gttcgtctag cttttgctgt
    72961 agcaatcaat gtgaatcccg atattttgat tgtggatgaa gcattggcag taggcgatct
    73021 tgaatttcaa ctaaaatgta tggaaaaatt tacagaaatc aagaactcgg gaaagacgat
    73081 tttattcgtt tctcatgata tcagttcgat tcgtcgtttt tgtgatcgtt cctattggtt
    73141 gaaaaacgga gaagtcgtcg aatatggcga tacgatggaa gtaacgacga attatgaaaa
    73201 ctttttaaag aaaaaatcag ttaaaacagt ggatcggaat aaaaaccaag tagaacggtt
    73261 cgccgctcca gatatcgtcg acgtgacgaa agcggaattg ttggataaag acaaaaatcc
    73321 ggtggatatg ctggaacaag gagaaaaact atacgttaaa gtaacttatg aagtaaaaga
    73381 tgatacaatc aaaaatccgg ttttaggaat tgcgctacga acagtcgaca atttttatgt
    73441 atgcggactt aatactctac tagatggtat caagattcct tggaaaaaag gagagaatat
    73501 cttttatctg gagtatgaaa agttaggttt gttagctggt gaatattatt ttgacgttgc
    73561 ggtctttgaa gaaaatgcaa cggttccttt ggtatataag acaaagtaca tgaacttgtt
    73621 tgtcagcggt tcttatatcg gagaaggcat cgtggtctta gaccataaat gggaagaagg
    73681 gactcatagc aatgaaatat gattttgaaa tggacctaga tgaacaaagc agtgtgggaa
    73741 aaattgccgc acagatcaaa ccaggaagta aagttttaga gtttggtccc ggaaacggcc
    73801 gattaacgaa acatttaatc ggagcaaaac aatgtgaagt gagcattgtt gagttagata
    73861 aggaattgtt cgattttgtc agcgaatttg ctcaagatgg attttatggt gatatcgaaa
    73921 gttttgagtg ggcaaactat tatgctggac agacatttga ttacgtcttg tttgcggatg
    73981 ttttagaaca tttggtagat cctggaaaaa cattgaagaa agtcagagaa tttttgaatg
    74041 aagaaggaga gatcctgatt acattcccta acttggcgca taattctgtc atgatcgatt
    74101 tgttcaataa ccaattgccg tgggcttctt atggtctcct agatgaaacc cataattctt
    74161 tttatactca tgatggcttc caaaaagttt ttgaaaaagc gggattattc attaatattg
    74221 aagattattt gtacttagct gtaggtgata cagaattaaa atcaacttat gaagaattgc
    74281 cggaagctgt acgctatgat ttcaaaatgc gaccatttgg tgaagtctac caatactttt
    74341 tctcattgat gaaacatcca gtagcacaaa gttccattgc agagccacaa aattctaatt
    74401 atgtaaaagt gttagaagta acccagcaaa cgaaacaaga tgaaacaatc caacaaatac
    74461 cattcaataa tttcacaggg gaaaatgaga cactaagttt ccccgtatca gaaacaacag
    74521 aaagaatggt gtttcgcttc gcgcaacaac ctagctttat cgaattcagc gcagaagctg
    74581 ccggagaaaa actaacattt atcgattcga atgctgtagt caaaacagta aatgattgtt
    74641 atttgtttga tgggaaagaa ttaccagagt tcgttctaac ggatatttct gggaaagaag
    74701 tgactatcca ttgtcactat cgctttatcg gggaattgac accaacaatg aaagaattgt
    74761 tggaaacgat tcgtcccatg gctgaagtca caaagcagct ttctgagaaa aatgacgaat
    74821 tagaaagaga aaatcatcat cttcttgagg aaaatacaag actagaccat acgttaaaaa
    74881 caaccacaaa tcgctattgc acattgttgg aaagtgatga atggacaata aaacatagac
    74941 taggacgacg taagaaagaa acttcgaaaa aaatccaaga aaaagaactt tctatatgta
    75001 ttgacgagaa aatctgggat gcagaaacaa aaatattaaa aatcatcgga tggggaatcg
    75061 ctaattcgga tcgccagccc ttgtcctata agttatcagc tgaccaatcg ccatttttcg
    75121 aagcaattcc tgtttcacga gaagaagtca atcaagcaga acagctcgca aaaggaacag
    75181 aagcgggatt tgagttacga atcctttgcg agcaagaacg ttcattctta gtggaagcag
    75241 tagctcaaaa tggccaatca tggatcattg aaatgtaaat aatgattaac ttataaagat
    75301 aaggagtaaa cgaatcgtgc aaaaaaaaga aattgccgta tttatcgatc aaatcactcg
    75361 tgaacgggcc actggcgatt tgcttattgt gggctgggca attgatgaag tgaccaaaga
    75421 aatccctacg ataaaggtag aaaaagaaaa tgtgattgct gaagcaacac atgtcgtaag
    75481 attggatatc aatcatttat ataatcttga tgttaagacc caaagcggtt tcaaggtacg
    75541 tttatcaggg aaaatgagag gaaaagccat cttagacttc caaacagcga gacatcaaaa
    75601 tggcatagct gtgaaattaa acggtaaata cccttatgat gacggcattg aatcttcatg
    75661 ggaaagaaaa aaaagattac tgaaaaaagg gataaattat gcaaggactc atggcgtaaa
    75721 aaaagcgatc cgtcgggtca aattggaatt gaagccagga tcgatcgact atgcagagtg
    75781 gatcagtcgt catgagaaac cagatttgaa agagcaaagg aaagaagcac aaggatttgc
    75841 gtaccgtcca ttgatttcca tacttatgcc tgtctataac gttgaaatca aatggctcga
    75901 aaaatgcatt gattcggtac tggatcaaac gtatgatcat tgggaactat gtatcagtga
    75961 tgatgcttct actgatccag ctatccgaaa gtgtctggaa tcctatcaag caaaagatga
    76021 tagaatcaaa gtcgttttcc gaaaagaaaa tggacacatc agtctggcaa ctaattctgc
    76081 attggaaata gcagaaggag aatttatagc actcttagat aatgatgatg aattaccgcc
    76141 atttgcatta tatgaagtgg caaaagtgct aaacgtacat ccagaactgg atgtgatcta
    76201 cagtgacgaa gataaaatcg atgcagatgg caatcgcttt gatccgcatt tcaaagcaga
    76261 ctggtcgcct gatacattga tgggaaataa ttatatctct cacttaggcg tatatcgaac
    76321 aagcattgtt aaagaacttg gcggtttccg taaaggctat gaaggttctc aagattatga
    76381 tttggtcctt cgtgtgacag aacaaatccc tgcagatcat atttaccaca ttgaccgagt
    76441 cttgtatcat tggcggacga tcccaggatc aactgcaagt aatggcgaag ccaaaagtta
    76501 tatctatgat tccggagtga aagcattgac agatgccctg tctagaagaa acatcaaagg
    76561 aagcgtacgt cctggcagaa tttctggatt ttatgagatt gcttatgacg tcttgcaaga
    76621 ggatttggtc agtgtgatca ttccaacgaa gaatggctat gaagatctaa aaacttgtgt
    76681 ggattcgatc atcgaaaaaa cgtcttattc aaattatgaa atcattattg cggataacgg
    76741 aagtacagat ccaaaaatgc aagaattatt tgcagaatac aagcatcagc tgaaagaccg
    76801 ctttatcgta gaattgattg acattccatt caattattct cgaattaaca atttagcagc
    76861 agaaaaagca aacggcaaat actttttatt cttgaataac gatacagaag tgattgagcc
    76921 agattggatg acggtgatgg tttcttatgc acagtttgat cgaatcggct gtgtaggagc
    76981 aaaactgttt tatccagatg atacaacgca gcatgcagga gtattattag gaatcggtgg
    77041 tgttgcaggg catgcattga ataactatga tcgaacacat tgtggttatt tcggccgatt
    77101 ggtcattgac gtcaattact tagcagtcac tgcagcttgt atgatggtca aggcagctga
    77161 cttcaatgct gttaacgggt ttgatgaaac attagaagta gcattcaacg atgttgactt
    77221 gtgcttgaaa gtttatgaac tgggtcgtta caatgtctat gcacatcaag cagaattgta
    77281 ccatttcgaa tctaaatcaa gaggttatga agatactcca gaaaaacaaa gacgttttgc
    77341 tggtgaaatc aaaaaaatgc aagataaatg gccagcatat attgcgcatg atccatttta
    77401 caatgataat ttaacaaaag acggaatcgg cgacttttcg ttgagaccag attaaactat
    77461 aataaaaagc tgttatgttg tgattcgctc gcacataacg gctttttata gttttttgtc
    77521 aaaaaaaggc agaaggtgcc gactcaaaaa aataagtcga ggttgggaca gaagtgttta
    77581 actccgagaa ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcggc
    77641 ttatttaaga agaagttgct tctgctccca ccgtttattc gtttttaaag aatgagacaa
    77701 aagtgtttct tacttttgtc ccacgctctt attcatgctc agagcgccaa tagatacaaa
    77761 atcatagcca aagtttcacg aaccctagct gtcaaagaat cgatatttta ttttaatata
    77821 ggctatttta aaataaatgg tttaaggtat tcctctacaa aagccactct atcaattatc
    77881 atgggtttaa aataagaagt gatggagata accagattgt tcttagcgtc aatataaaga
    77941 acatttccac tattaccaat tgctgaatat atttcgttct cagaatctaa tacccaccat
    78001 aaatagccat atctcatatt ttgtaagtct tctaaagcac tggttctagg tttgaacatc
    78061 tcgtttatcc acatgggtgg tacgatttgt tttcctttat acatcccttt atttaggcat
    78121 aatacaccaa tttttgccag atcacttgcc gataaacaca aaccatatcc tgccgtcccg
    78181 atatttagtg gatcacaaaa ccagacattg gttttaggag acttatccat tgtaaactgc
    78241 ttatgttctt cagctgtttc agcataataa ttgacatggt ttggtatccc tataggatca
    78301 aatagatact tattggcaaa atcaacagtt gttagcttac ttgtctgata caacaagcca
    78361 gttaatatgt gtaaacagac agtttgatag ttgaattcgc cggttatacc ttttcttcct
    78421 cccaataaat ctagtgatgt ttttgtccaa tcttcacttg aacagacttt tgtccatgga
    78481 tcgcctttac atttgtaggg tgctttcatt gttagcaagt gcttgattgt aatagcgtaa
    78541 atagttttct ccccgcgttt cactttatac tctggaaaat aatctaatac tttatcatcg
    78601 atacttttga ttaatccttg ctctaaagca atgccaatca gcaaagatac aatacttttc
    78661 gttgctgaca tgatatgaca agtatcgtct tttttataac cattccattc atctgtataa
    78721 acttctaccc catccttata tgcgacaatt tgacatatat ttggttgtcg aaaaccaata
    78781 aaatcatgca actcttgttt attcataaga tattacctca ctttcttcta taagcataat
    78841 gtcattaaaa atcgatttca attttttcga ttcttcacgt aatacaggta ttcttatgga
    78901 ctagacagcg ctttgggaag atggattctc tgaaaaatat aaaggttcac tttatgagtg
    78961 gataaaaaaa gcagcaaata cttcgacttc tttaatttac caacattagt gaaaatcgat
    79021 tcatctggag atttgtaatt tagagtcctt gtcttgatta gaggtgtata aagcttttta
    79081 ttagacattt ttaaattcca ttacaatttg cttataaact aggtttttaa aggtgactat
    79141 ggtaagataa tagagatatt attttgtgat taggagtgta tatatgaata aaaatggaga
    79201 atggagtgct gcacgacgga tattcattat attaatggat gttattgtat acaatgcagc
    79261 aatatatagt agtttcttac tgaagtttcg tggagagatc ccattacgaa atttagaaac
    79321 tttccaacac tcagcaatta ttatttcagt gatttttatt gcgttgaaca ttttgttggg
    79381 tgcatatgtt ttttataatc gaatgatcag tgacatcgtg tttgtcacag tcatcggtca
    79441 ggtattgatg acactaggga tcatggtagt gacttttgcg ggccgctggt ttgcttttcc
    79501 acgtcttgtt atcttacttt catttgttct cggtacgctt ttcttaagta tctatcgtat
    79561 tctcgtatat aaactttatt tacgcgtaag tcgagataaa aaggtcgtca ttattgggtt
    79621 agaaaaggat gtcgcaccag caatccaaaa ttttggttca aagaaaaaca acaaacataa
    79681 agtcgaagca gtcattcttg ataactatta tgcaaacatc aaaaatatga ttaaagacat
    79741 cgatattgtc tatcttgctt ctcatatcga agaaactgaa aaaatcaaaa tctatcaatt
    79801 agttactaaa aatgaaaaaa aattgttttt gaatacaaca tttgaaaact tgacattgat
    79861 caatccaaat attatgaatt tcgaagatga aagtattatt gaagcatctg gatttagaat
    79921 ccaacctgaa tatgaactat tcaaacgttt gtttgatatc atcgtttcac ttgttttatt
    79981 agttattgct tcaccattta tgttattcac agcaatttta gtcaaagtga catcaccggg
    80041 accaatcatc tataaacaag tgcggatcac aaaaaatcaa aaggaatttt caatctataa
    80101 attccgctcg atgaccgcca cagccgaagc aaaatctgga ccagttttag ctaagagcaa
    80161 tgatgcacga gtaacgcctg taggtaaatt tattcgagct gtccgctttg atgaattacc
    80221 acaaatcttc aatgtgctga aaggcgatat gtcgatcgta ggaccacgtc ctgaacgttc
    80281 tttcttcgtc gatcagttca atgcagaaaa cccttattac tacttacggc ataatgttcg
    80341 tgctggtatc actgggtatg cacaagtcta tggaaaatat gtctctgatt acaacagtaa
    80401 attacgtttt gatttacttt atatcaaaaa atattcattg atgcttgatt taaagatctt
    80461 gctgcaaacc atcaagatcc tatttgataa aatgtcctct caaggacttg aagaagacga
    80521 aaatgtgatc tcattggcag aaattgcttt tccgaatgaa aaaatctttc gctagttggg
    80581 aggcgtaaaa atgaacaatc cgaaattcag cataatcgta ccagtataca atgcagaagc
    80641 aactattggt cgaacgattg atattctaaa agaattggat tatgaagagt acgaagtcgt
    80701 tttagtaaat gatggatcaa cagatcaaac acaggaaatc attcaagaac tgattgaaaa
    80761 tgatgaacgt tttgaattga ttactacgac caatcaaggg cccggattag caagaaacga
    80821 aggaataaga cagtcaaaag gagaatatct cttattcttc gatgcagatg acacgccagt
    80881 aaaaacgatc ttgtcagatt atgatcattt gctagaaaag agtccaaaag cggatcttgt
    80941 tgttagttcg tttgtttttc gtacaatgga cgagggaaga atcgtttccg aaaaagaaaa
    81001 cttagttaca gaatgtcact atctaactca cgaagagttt ataaaagaca tgtatcagtt
    81061 aatgaatgaa cagttgatgt atgttgtatg gaacaaatgc tatcgtagcg atattctgaa
    81121 aaataaccaa attctattta aaacttataa tagttgtgag gatcgaatat ttaacttaca
    81181 ttattatgaa tactgtcatc aagtgatcat gaacccaaaa gtagaatata tttatgaatt
    81241 tgaaggtggg aaggggatca ctaatcaata ccgacctaac aagttcgaaa catttaaaga
    81301 attctattca ttagcaaata ctgtaacaga cgatattaat aaagacggta tggcggcgct
    81361 tcttttaaaa gggacaactt cagttatttt ttctatttat agtacctctg caagaacttc
    81421 aaaagagaag aaagacgaag caaagcagat attgagtgat ccagtgatcc gagaagccaa
    81481 gcaaattgct aagacggatt cttcagcaaa aaaaataaca aagttgcttt acaatatgcc
    81541 agctccacta tttttatctg ctgtaaaagt tggaagcttc gttgaagtga aaatgccagg
    81601 gttaatggct gctttgaagc gagtatatta gttaatggag gaaaatataa tgaaaagaat
    81661 tttgcatttt cagggacgaa tgggtttagg tggagcagag tctttcatga tgaatttata
    81721 tcgtaaaatt gatcgtacaa actatcaatt tgattttttg atttatgaag attatgcaga
    81781 tgttcaagac tatcattcag agattgaaag attaggaggc cgtatttttg ttgtacctaa
    81841 tcctaagaaa aatattttta aatatttaat agaagtgaat aagttactaa aaaaagaatc
    81901 tttttctata gttcacaatc aggtttattt tggcggggga attaatttat ggttagccaa
    81961 aaagaatgga atcagacaga gaattgctca tagccatgca actgaagatg gaaaatctca
    82021 aaatattgta atgaatgtat tgcgtaaatt tttaaccaaa ttgttgttac aaaatgcaac
    82081 agactacctt gccgttagtc aagaagctgg tgaaagtctg tttcaaaacc atccctttga
    82141 gatagttcat aatggtattg atttagagct ttatagtaaa aattcagaag ctaaggttaa
    82201 taagagaaaa gaattggata tctcaatgag tacttttgtt gtgggaaaca ttggacgatt
    82261 agagaaacaa aaaaatcaat cttatttgtt agagattttt ttagaaatac taaaaatgaa
    82321 ttctgattct tacttgattt tagtaggtga agggtcactt agaaataaac tgaaagccaa
    82381 agcagtagaa ttggatatag agaaaagagt aaaattttta ggagaaagaa acgatattcc
    82441 cgagttatta tctgtgatgg atgtttttgt aatgccttct ctttatgaag gtctgccaat
    82501 ggttggatta gaagcacaag cagcaaaatt aaaattagtc ttagcagata ctatttccaa
    82561 agatacaaag ttaacaaata atgtggattt tgtatcatta gatgaaacac cacaagaatg
    82621 ggcaaaaaaa gtaataaaag agccgtatgc taataaaata acacaagaac taaagagtta
    82681 tgatgtttcg tacactactg aacaaatgga gagtatatat agtagagatc attaaaaatg
    82741 ttcaagatat tttaggagtg attatttttg aaaatatgtt atttgatatt ggcatacaat
    82801 gatattggca acttaaagag attggtcaat cgattgaacg aaaatgcagt ttttattatt
    82861 catatagata aaaaaactga tatagaacca tttattgaag catttaaatc atatgataat
    82921 gttgaatttt tatgtaataa aaaccgtgtt aaaatttatt ggggtggttt ttccattgta
    82981 caagcagaaa tcaatttagt aaagagagca ttacaaaatg agaaatattt aaaatatgta
    83041 tt
//
LOCUS       DARBFD010000005        60875 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000005 DARBFD010000000
VERSION     DARBFD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.005"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="SCK08_000321"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="SCK08_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEG1200239.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="SCK08_000322"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="SCK08_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEG1200240.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="SCK08_000323"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="SCK08_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEG1200241.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="SCK08_000324"
     CDS             4735..5628
                     /locus_tag="SCK08_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200242.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="SCK08_000325"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="SCK08_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1200243.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="SCK08_000326"
     CDS             7565..8422
                     /locus_tag="SCK08_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEG1200244.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="SCK08_000327"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="SCK08_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEG1200245.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="SCK08_000328"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="SCK08_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEG1200246.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="SCK08_000329"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="SCK08_000329"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1200247.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="SCK08_000330"
     CDS             11178..11936
                     /locus_tag="SCK08_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1200248.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="SCK08_000331"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="SCK08_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEG1200249.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="SCK08_000332"
     CDS             12864..13895
                     /locus_tag="SCK08_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1200250.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="SCK08_000333"
     CDS             13892..14659
                     /locus_tag="SCK08_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1200251.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="SCK08_000334"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="SCK08_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEG1200252.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="SCK08_000335"
     CDS             16624..16944
                     /locus_tag="SCK08_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEG1200253.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="SCK08_000336"
     CDS             16944..17294
                     /locus_tag="SCK08_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEG1200254.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="SCK08_000337"
     CDS             17461..18399
                     /locus_tag="SCK08_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEG1200255.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="SCK08_000338"
     CDS             18414..19244
                     /locus_tag="SCK08_000338"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEG1200256.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="SCK08_000339"
     CDS             19288..20313
                     /locus_tag="SCK08_000339"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1200257.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="SCK08_000340"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="SCK08_000340"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1200258.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="SCK08_000341"
     CDS             21391..21831
                     /locus_tag="SCK08_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEG1200259.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="SCK08_000342"
     CDS             21834..22436
                     /locus_tag="SCK08_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1200260.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="SCK08_000343"
     CDS             complement(22495..23601)
                     /locus_tag="SCK08_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1200261.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="SCK08_000344"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="SCK08_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEG1200262.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="SCK08_000345"
     CDS             complement(25149..27494)
                     /locus_tag="SCK08_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1200263.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="SCK08_000346"
     CDS             27773..28675
                     /locus_tag="SCK08_000346"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEG1200264.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="SCK08_000347"
     CDS             28888..30663
                     /locus_tag="SCK08_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEG1200265.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="SCK08_000348"
     CDS             complement(30729..32315)
                     /locus_tag="SCK08_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEG1200266.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="SCK08_000349"
     CDS             32566..32790
                     /locus_tag="SCK08_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEG1200267.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="SCK08_000350"
     CDS             32932..34683
                     /locus_tag="SCK08_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1200268.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="SCK08_000351"
     CDS             34683..36467
                     /locus_tag="SCK08_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1200269.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /locus_tag="SCK08_000352"
     CDS             36793..37644
                     /locus_tag="SCK08_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1200270.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37698..39449
                     /locus_tag="SCK08_000353"
     CDS             37698..39449
                     /locus_tag="SCK08_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1200271.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39453..39623
                     /locus_tag="SCK08_000354"
     CDS             39453..39623
                     /locus_tag="SCK08_000354"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1200272.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39663..40565)
                     /locus_tag="SCK08_000355"
     CDS             complement(39663..40565)
                     /locus_tag="SCK08_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1200273.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="SCK08_000356"
     CDS             40796..42199
                     /locus_tag="SCK08_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1200274.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="SCK08_000357"
     CDS             42199..43632
                     /locus_tag="SCK08_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1200275.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="SCK08_000358"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="SCK08_000358"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEG1200276.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="SCK08_000359"
     CDS             44917..46644
                     /locus_tag="SCK08_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEG1200277.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="SCK08_000360"
     CDS             46659..47009
                     /locus_tag="SCK08_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1200278.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="SCK08_000361"
     CDS             complement(47101..47961)
                     /locus_tag="SCK08_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEG1200279.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="SCK08_000362"
     CDS             48053..48481
                     /locus_tag="SCK08_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1200280.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /locus_tag="SCK08_000363"
     CDS             48643..48819
                     /locus_tag="SCK08_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEG1200281.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..49292
                     /locus_tag="SCK08_000364"
     CDS             48846..49292
                     /locus_tag="SCK08_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEG1200282.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="SCK08_000365"
     CDS             complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="SCK08_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1200283.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            51531..52502
                     /locus_tag="SCK08_000366"
     CDS             51531..52502
                     /locus_tag="SCK08_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEG1200284.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            52526..54718
                     /locus_tag="SCK08_000367"
     CDS             52526..54718
                     /locus_tag="SCK08_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEG1200285.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            54735..55211
                     /gene="ybeY"
                     /locus_tag="SCK08_000368"
     CDS             54735..55211
                     /gene="ybeY"
                     /locus_tag="SCK08_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEG1200286.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            55195..55590
                     /locus_tag="SCK08_000369"
     CDS             55195..55590
                     /locus_tag="SCK08_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEG1200287.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            55605..56504
                     /gene="era"
                     /locus_tag="SCK08_000370"
     CDS             55605..56504
                     /gene="era"
                     /locus_tag="SCK08_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEG1200288.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            56647..57459
                     /gene="recO"
                     /locus_tag="SCK08_000371"
     CDS             56647..57459
                     /gene="recO"
                     /locus_tag="SCK08_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEG1200289.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            57843..58760
                     /gene="glyQ"
                     /locus_tag="SCK08_000372"
     CDS             57843..58760
                     /gene="glyQ"
                     /locus_tag="SCK08_000372"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEG1200290.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            58762..60837
                     /locus_tag="SCK08_000373"
     CDS             58762..60837
                     /locus_tag="SCK08_000373"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEG1200291.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        19863 a        10760 c        13027 g        17225 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat
    49321 tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa
    49381 gattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa ttgtgtttga
    49441 tttggttatt cttctggtag ttcatttgct tcttgtttat accctgatag gcgataggcg
    49501 taataaaaga gtaagctgcc agcgtaacca agtaacgttg ggaccatcca gccaagtcct
    49561 aatgcagcaa aaggaacgtg atgatgataa aatcctagta agcttgctac gacagtttgc
    49621 tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac ggcagaaaag
    49681 aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt taaggaaagc
    49741 aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga ccattggatg
    49801 atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa ccgcagccat
    49861 gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga aacaacgagt
    49921 cccatagcgg tagtgaagac acctaatgtg accattaccc ctaagaagct agcaccgaag
    49981 ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc tgataaaggc
    50041 atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat gacttctaac
    50101 gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc tttaaaacca
    50161 agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac tgcatcaacc
    50221 gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga atctgcagca
    50281 gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat agctaataat
    50341 gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt tggatgtaca
    50401 gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc taatgaatca
    50461 tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc tgtccgcggt
    50521 gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc agcataccat
    50581 tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac tacagctaag
    50641 atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc gctgatccag
    50701 tttgatccag ccatttgacc taagtggaca ggaaagatca gattacctga cccgaagaac
    50761 atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt ttttgtttct
    50821 tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg ttgcagaaac
    50881 tagaaatgcc actaatatca gattccatga aaaccaccac cttaaaatat aaaaaaaagc
    50941 ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa
    51001 aaaagcttct tcattcacgg acgcttttcg ataaagttat ccgctagcct gataatggag
    51061 gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt
    51121 tattgtcatt tctcacttac atgactcact gggaataaat cgctgaatag cctactgttt
    51181 tcctcgacgc tttgctgtat atttatttgt tgacgttata atacacctgt tttttttgaa
    51241 atgcaagaat atttcaaata ttttgttttc tttttttgaa acaagaaaaa tacccagaga
    51301 atacttgcta agttagacgg aatacttgtc ctctatctta tgcatagctt acatgatcag
    51361 ctttttttat tcaaacgaga cctttagaga atggaagcag ctatttttta acgtttcttg
    51421 gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata gtttcttctg
    51481 agtatggtaa aataggaaag aatgaatcga aaaaaagggg atgtctattt ttgacggaag
    51541 agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg cttttaggtg
    51601 cacacgacaa gcatataaaa ctgattgaag aaacaacaga aacaaccatc catacacgtg
    51661 gagaaatcat tcagatcatt ggcgaacaat cccagatttc cttggctgcc tcagtcattc
    51721 ggacattgca ggaattgatc aaacgaggaa tccatgtaag tacaccagat gtcgtgacgg
    51781 cactgaaaat ggggcaaaaa ggcacattag attattttgt tgaaatgtat gaggaagaga
    51841 tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa aagaaatatg
    51901 tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg acaggaaaaa
    51961 cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg caaaaaatca
    52021 ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg ctttcttccc ggggatctaa
    52081 aagaaaaagt agatccttat ctgcgtcctg tttatgatgc tttataccaa atttttggat
    52141 tagatcatac gaaccgattg atggaacgag gcgtgatcga aattgctcct ttagcttaca
    52201 tgcgggggcg gacattggat gatgcattcg ttattttgga tgaagcacag aacacgacag
    52261 ttgctcagat gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg attgtcaatg
    52321 gagatacgag tcagattgat ctgcctcgag ggacaacaag tggtcttgtt catgcagaac
    52381 gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc
    52441 ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc catcaagaat
    52501 aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa
    52561 acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta tctgcggcgt
    52621 tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag tagcagaaga
    52681 aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa aaagaaaact
    52741 ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag atgaccagaa
    52801 caatcggatc aagcaactat ttgaattgat cgataaaacg aatgatgcca tcaacaaatc
    52861 ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc ctactacaga
    52921 agagcggatc gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa atgttgcgtt
    52981 ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg aaactgagat
    53041 ccatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca aacaagttcg
    53101 tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat atttgaatgt
    53161 cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg tagtgaatga
    53221 cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag ctgtacaacc
    53281 agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg atgcttcagc
    53341 tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt ttccgctaat
    53401 tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt cacttcaatt
    53461 ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta tgtcagtcag
    53521 catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt atatcccgct
    53581 gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaacc gaagagcagg
    53641 gatcatggct ggtattttcc aagcagtatc tgcacttttc attttttatg attcgattgg
    53701 cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca tggcaacgat
    53761 tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg tcattatttt
    53821 tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg gtgatgcaac
    53881 gacttggtta atgcttgtct gcgggttcac tggtgcactc ttctcattct tgctgacgat
    53941 cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg tattaaatga
    54001 actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc caggtactta
    54061 tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa tcggcggacg
    54121 ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca aacatgctag
    54181 ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg cacaatttct tattgccaga
    54241 agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat tggaagatta
    54301 taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca cgctaatgcg
    54361 ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc aattccgtta
    54421 tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg acagttgtga
    54481 ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa
    54541 tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga ccttgaaaga
    54601 aatccgaaaa gtggaaaaat cattgatcag cggtttaagt agcaccttcc attctcggat
    54661 caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagaacaag aagaatagaa
    54721 agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc tcagaagaga
    54781 aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg
    54841 aaacagaaat gtctgttact ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt
    54901 accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa gaaggcgaag
    54961 acgaaatccc agtgattttt gaagatggag agaatccatt gcctcgtgta ctaggagaca
    55021 tcatgatttc tattgaacgt gctgctgaac aagcagaagc ttacggccat tcatatgatc
    55081 gggaacttgg ctttttagcg gtacatggat tcttgcatat caacgggtat gaccatatga
    55141 caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat gcctatggac
    55201 tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga atttgctgtc
    55261 caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt
    55321 atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagagtg gttatggctg
    55381 cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt tgaaaatgta
    55441 gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa agatatggca
    55501 gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat tttatttgtc
    55561 cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca gaacataaat
    55621 cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta ttgaaccgaa
    55681 tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga aataaaatcc
    55741 aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca ggaatccata
    55801 aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg ttgaaagaag
    55861 tggatgcagt cttatttatg atcagtgctg atcaaaaacg cggccgggga gacgatttta
    55921 ttattgaacg tctaaaaaac gtgcattctc cagtttatct gattatcaat aaaatcgata
    55981 agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag atgccttttg
    56041 cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta atggatgtat
    56101 tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg actgatcatc
    56161 ctgaatactt tatcgtttct gaattgatcc gtgagaaagt cttgttttta acacgagacg
    56221 aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa
    56281 tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc attatcggca
    56341 aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt
    56401 taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg cgagacaaaa
    56461 aagtctatct gcaagacttt ggctaccgaa aagacgaata ctgatattct gaacttctta
    56521 taggttaagt atagaaataa actggataaa aatccgcacg ttgagaaaaa cggcggaaga
    56581 tcgaaacata aataatctgg atcggcagaa tctgcttttc cagatttatt ttttaggagg
    56641 gagaatatga gccagcttgc agaatcgaaa ggcttgattt tatctagccg taattacaaa
    56701 gaaaaagata aattagtcaa aatctttacg gaatcttctg gcaaaatgat gttttatatc
    56761 aaaggaatcc atcggcaaaa tcagccatta gctccggctt tgctcccctt taccgaagca
    56821 gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc
    56881 catgcgtttc ggaacatcca agaagatatc tttttgagcg gttatggtac ctatttgcta
    56941 aatctagtag atgcagcaat cgaagatcat caatatgacc cgcatttatt ccaatttacg
    57001 caacaagctc tgcagcggat ggatcaagga gatgatgcag aaatcatcac gaatatcttt
    57061 gaagttcaaa tactgcaacg cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc
    57121 ggggaaacaa aaggaaaatt tgactattct tccaaatctg gcggtgtgat atgcgagaaa
    57181 cactggccaa tggacgaaca ccgttatcat gcagatcctc gcgctgttta tttcgttcga
    57241 atgttttcgg ctatttctta tgataagatc agtggaatcc acttgaaaga agaaacgaag
    57301 caggcaatcc gtgacctcat tgaccagttg tatgatgaat atgtcggtat tcatctaaaa
    57361 agcaaaaagt tcattgatca aatgaagtct tgggcacacg tattaaagac gccagagaaa
    57421 aatatgcctg aaaaagagat acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa
    57481 tcaaaactat ttgacaaata cagggacttc cgtctattat taaccgtaga ttaaatagat
    57541 cgttcactga gaggatctat tatataaaca agaaaactgc aaaatgaata cgtacattga
    57601 ccaagatcag taaaaaacac gtggtttagc gagttcggga gagtggaagc cggacccaaa
    57661 cgatttttga agaggcactt gcgagtacga taaatgaagc tgccgaatct ttattcggaa
    57721 gtagggtgga accgcgaaat tattcgtccc tatgtctgta atcagacata gggacttttt
    57781 gtcgttttct ttcctgtatt cttattgaca gcaacgctta aaaattgaaa ggggcaaaac
    57841 agatgagtaa aaaattgacc gtccaagata tgattttaac attacaaaaa ttttggtcat
    57901 caaacggctg catgctgatg caagcttatg acacagaaaa aggagcaggt acgatgagcc
    57961 catatacatt cttacgggca atcggaccgg aaccatggaa tgctgcttat gtagagccat
    58021 cccgccgacc agctgacggg cgttatggag aaaacccaaa ccgtttgtat cagcatcatc
    58081 aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca agagctttat ttagaaagct
    58141 tgcgtctttt aggaattgat ccattagaac acgatattcg ttttgttgaa gacaactggg
    58201 agaacccatc gatggggtgt gccggtgtgg gttgggaagt atggcttgat gggatggaga
    58261 tcacacaatt cacgtacttc cagcaagtcg gaggactacc ttgtaaacca gttacttctg
    58321 agatcactta tggactagaa cgtcttgctt catatattca agaagttgaa agtgtctatg
    58381 atctggaatg ggctgatggt gtgaaatatg gcgagatctt caaacaacca gaatatgaac
    58441 attccaaata ttcttttgaa gtaagtgacc aagacttgct actaagcaac tttgaccgtt
    58501 ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg tatgattata
    58561 ttttgaaatg cagccataca ttcaatttat tagatgctcg cggtgcagta tccgtaacag
    58621 aacgagcagg ttatctttcc cgtatccgca acatggctcg tgctgtagcc aaaatctttg
    58681 tggcagaacg cgaaaaatta ggattccctt tattgaataa aaacacagca aatccagaaa
    58741 acatgaagga ggctgaataa gatggcaaaa gacttattac ttgaaatcgg gctggaagaa
    58801 atgcctgctc atgttgtcac accaagcatg aaacaattag aagaaaaaac agcaaaattt
    58861 ttagatgaaa accaattgac ttatgatacg atcgaaacgt tttctacacc gcgccgctta
    58921 gcaatcaaag taacaaacat tccagaacgt caagcagaca gtgaagaaga agtcaaagga
    58981 ccagccaaaa aaattgcttt ggacgcagat ggaaactgga cgaaagcagc tgaaggattt
    59041 gtccgcggac aaggtctgac gacagatgct atcactttta gagaattaaa aggcgtggaa
    59101 tatgtctatg taacgaaata cactcacggc aaagaagcga aagaagtatt gacaggttta
    59161 gcagatgtca ttaaaagcct cactttccca gtaacgatgc attgggcaga atacgatttt
    59221 gaatatatcc gccctattca ctggatcgtg gcattgttag atgacgaagt aatcccgttt
    59281 gagatcttag atgtcaaaac aggcagagcc tctcgaggac atcgtttctt aggcgaagat
    59341 gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga atgaacaatt cgttattgct
    59401 tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa atgaattagc cgaaaaaaat
    59461 cactgggtta ttgatctaga cgaagattta ctagaagaag tcaacaattt agtcgaatat
    59521 ccaactgtct tttctggcga cttcgaagaa aaatatcttt ctgttcctga agaagtacta
    59581 gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac ggaacgatca aggtatgctg
    59641 cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa aattggaaaa tgtggttcga
    59701 ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg aattctttta taatgaagac
    59761 aagaaagctt caatcgacca atacgttgaa aaactaaaaa ctgttacttt ccatgaaaaa
    59821 atcggtagta tctatgagaa aatgcagcgg gtacaagtca ttgcccgtat cattggcgaa
    59881 caagtaggac tatctgagga agaattgatg gatctgcaac gagctgctga aatctataaa
    59941 tttgatctag taaccaacat ggtaggggaa tttcctgaac tacaagggat catgggcgaa
    60001 aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa cagcgattcg tgagcattac
    60061 atgcctatat ccagtgaagg agagctacct cagtctacag taggttctgt tttagcgatt
    60121 gcagataaat tagatagcat tttttctttc tttgcagttg ggatgattcc aagcgggtca
    60181 aacgatcctt atgccttacg tcgccaagca tacggtgtcg ttcggatcat cgaatcgaaa
    60241 aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta gtgatgttat cagtaaagat
    60301 acggatcgtt tcggcattgg tttatcagaa gggcaacaac aggtgatcga ctttatcaaa
    60361 ggccgtctgc gccaattatt gacaacgaag aatattcgtc atgatgtcat cgaagctgtt
    60421 ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt gttcaagcag
    60481 catcttgcag atgaagactt caaaccatcg atggaagctt tgacacgtgt cgtgaactta
    60541 gcgaaaaaag cggagcttga aaaacaatca gaagtagacc ctgaactgtt cgaaaatgaa
    60601 gcggagaaag aactgcacaa agcagtagag gcaatgaaaa aaagctttgc tgaccaaacg
    60661 atcaacgaaa attatgatca gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat
    60721 cagacgatgg tcatggcaga agaaccagca gtacgtaaga accgattaac gcaattaagt
    60781 caaattgctg ccatggcttt gtcgctagca agtttagatc aaatcatcac aaaataattt
    60841 ctaatatgta aataaaccag agaataggca caaaa
//
LOCUS       DARBFD010000006        60190 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000006 DARBFD010000000
VERSION     DARBFD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.006"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(112..471)
                     /gene="rplT"
                     /locus_tag="SCK08_000374"
     CDS             complement(112..471)
                     /gene="rplT"
                     /locus_tag="SCK08_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEG1200292.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(524..724)
                     /gene="rpmI"
                     /locus_tag="SCK08_000375"
     CDS             complement(524..724)
                     /gene="rpmI"
                     /locus_tag="SCK08_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEG1200293.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(796..1320)
                     /gene="infC"
                     /locus_tag="SCK08_000376"
     CDS             complement(796..1320)
                     /gene="infC"
                     /locus_tag="SCK08_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEG1200294.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1364..1489)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1640..2272
                     /locus_tag="SCK08_000377"
     CDS             1640..2272
                     /locus_tag="SCK08_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1200295.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2274..3179)
                     /locus_tag="SCK08_000378"
     CDS             complement(2274..3179)
                     /locus_tag="SCK08_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1200296.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3264..3923)
                     /locus_tag="SCK08_000379"
     CDS             complement(3264..3923)
                     /locus_tag="SCK08_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1200297.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4239..5183
                     /gene="mvk"
                     /locus_tag="SCK08_000380"
     CDS             4239..5183
                     /gene="mvk"
                     /locus_tag="SCK08_000380"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEG1200298.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5176..6153
                     /gene="mvaD"
                     /locus_tag="SCK08_000381"
     CDS             5176..6153
                     /gene="mvaD"
                     /locus_tag="SCK08_000381"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEG1200299.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6166..7251
                     /locus_tag="SCK08_000382"
     CDS             6166..7251
                     /locus_tag="SCK08_000382"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEG1200300.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7248..8312
                     /locus_tag="SCK08_000383"
     CDS             7248..8312
                     /locus_tag="SCK08_000383"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEG1200301.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8561..9364)
                     /locus_tag="SCK08_000384"
     CDS             complement(8561..9364)
                     /locus_tag="SCK08_000384"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEG1200302.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="SCK08_000385"
     CDS             complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="SCK08_000385"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEG1200303.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10242..11249)
                     /locus_tag="SCK08_000386"
     CDS             complement(10242..11249)
                     /locus_tag="SCK08_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1200304.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11256..11900)
                     /locus_tag="SCK08_000387"
     CDS             complement(11256..11900)
                     /locus_tag="SCK08_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG1200305.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12000..12758)
                     /locus_tag="SCK08_000388"
     CDS             complement(12000..12758)
                     /locus_tag="SCK08_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEG1200306.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="SCK08_000389"
     CDS             complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="SCK08_000389"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEG1200307.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="SCK08_000390"
     CDS             complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="SCK08_000390"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEG1200308.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="SCK08_000391"
     CDS             complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="SCK08_000391"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEG1200309.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="SCK08_000392"
     CDS             complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="SCK08_000392"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEG1200310.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="SCK08_000393"
     CDS             complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="SCK08_000393"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEG1200311.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18156..19025
                     /locus_tag="SCK08_000394"
     CDS             18156..19025
                     /locus_tag="SCK08_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEG1200312.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19151..20050)
                     /locus_tag="SCK08_000395"
     CDS             complement(19151..20050)
                     /locus_tag="SCK08_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1200313.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20215..21039)
                     /locus_tag="SCK08_000396"
     CDS             complement(20215..21039)
                     /locus_tag="SCK08_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1200314.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21050..22345)
                     /locus_tag="SCK08_000397"
     CDS             complement(21050..22345)
                     /locus_tag="SCK08_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEG1200315.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22361..22840)
                     /locus_tag="SCK08_000398"
     CDS             complement(22361..22840)
                     /locus_tag="SCK08_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEG1200316.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22846..23874)
                     /locus_tag="SCK08_000399"
     CDS             complement(22846..23874)
                     /locus_tag="SCK08_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEG1200317.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23891..24997)
                     /locus_tag="SCK08_000400"
     CDS             complement(23891..24997)
                     /locus_tag="SCK08_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1200318.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="SCK08_000401"
                     /gene_synonym="adhC"
     CDS             complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="SCK08_000401"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEG1200319.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="SCK08_000402"
     CDS             complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="SCK08_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEG1200320.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="SCK08_000403"
     CDS             complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="SCK08_000403"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEG1200321.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29968..30666
                     /locus_tag="SCK08_000404"
     CDS             29968..30666
                     /locus_tag="SCK08_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1200322.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30834..33473)
                     /locus_tag="SCK08_000405"
     CDS             complement(30834..33473)
                     /locus_tag="SCK08_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1200323.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34092..35123)
                     /gene="queA"
                     /locus_tag="SCK08_000406"
     CDS             complement(34092..35123)
                     /gene="queA"
                     /locus_tag="SCK08_000406"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEG1200324.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35316..36491
                     /locus_tag="SCK08_000407"
     CDS             35316..36491
                     /locus_tag="SCK08_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1200325.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36495..37136
                     /locus_tag="SCK08_000408"
     CDS             36495..37136
                     /locus_tag="SCK08_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200326.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37133..38050
                     /locus_tag="SCK08_000409"
     CDS             37133..38050
                     /locus_tag="SCK08_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1200327.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38047..38718
                     /locus_tag="SCK08_000410"
     CDS             38047..38718
                     /locus_tag="SCK08_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200328.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39259..39747)
                     /locus_tag="SCK08_000411"
     CDS             complement(39259..39747)
                     /locus_tag="SCK08_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEG1200329.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39948..40214)
                     /locus_tag="SCK08_000412"
     CDS             complement(39948..40214)
                     /locus_tag="SCK08_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1200330.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40214..40477)
                     /locus_tag="SCK08_000413"
     CDS             complement(40214..40477)
                     /locus_tag="SCK08_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1200331.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40953..41138
                     /locus_tag="SCK08_000414"
     CDS             40953..41138
                     /locus_tag="SCK08_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1200332.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41280..43103)
                     /locus_tag="SCK08_000415"
     CDS             complement(41280..43103)
                     /locus_tag="SCK08_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEG1200333.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43701..46406)
                     /locus_tag="SCK08_000416"
     CDS             complement(43701..46406)
                     /locus_tag="SCK08_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEG1200334.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46712..47659)
                     /locus_tag="SCK08_000417"
     CDS             complement(46712..47659)
                     /locus_tag="SCK08_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEG1200335.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47849..48118
                     /gene="rpsN"
                     /locus_tag="SCK08_000418"
     CDS             47849..48118
                     /gene="rpsN"
                     /locus_tag="SCK08_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1200336.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48294..48656)
                     /locus_tag="SCK08_000419"
     CDS             complement(48294..48656)
                     /locus_tag="SCK08_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1200337.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48667..49788)
                     /locus_tag="SCK08_000420"
     CDS             complement(48667..49788)
                     /locus_tag="SCK08_000420"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEG1200338.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49804..50154)
                     /locus_tag="SCK08_000421"
     CDS             complement(49804..50154)
                     /locus_tag="SCK08_000421"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEG1200339.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50284..51795)
                     /locus_tag="SCK08_000422"
     CDS             complement(50284..51795)
                     /locus_tag="SCK08_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1200340.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52117..53469)
                     /locus_tag="SCK08_000423"
     CDS             complement(52117..53469)
                     /locus_tag="SCK08_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEG1200341.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53622..54698)
                     /locus_tag="SCK08_000424"
     CDS             complement(53622..54698)
                     /locus_tag="SCK08_000424"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEG1200342.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54894..56639
                     /locus_tag="SCK08_000425"
     CDS             54894..56639
                     /locus_tag="SCK08_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEG1200343.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56919..57356)
                     /locus_tag="SCK08_000426"
     CDS             complement(56919..57356)
                     /locus_tag="SCK08_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEG1200344.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57340..58713)
                     /locus_tag="SCK08_000427"
     CDS             complement(57340..58713)
                     /locus_tag="SCK08_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200345.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59035..59523)
                     /locus_tag="SCK08_000428"
     CDS             complement(59035..59523)
                     /locus_tag="SCK08_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1200346.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59545..60147)
                     /locus_tag="SCK08_000429"
     CDS             complement(59545..60147)
                     /locus_tag="SCK08_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1200347.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17103 a        13016 c        10989 g        19082 t
ORIGIN      
        1 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
       61 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      121 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      181 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      241 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      301 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      361 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
      421 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
      481 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
      541 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
      601 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
      661 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
      721 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
      781 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
      841 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
      901 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
      961 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1021 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1081 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1141 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1201 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1261 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1321 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1381 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     1441 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
     1501 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     1561 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
     1621 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     1681 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     1741 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     1801 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     1861 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     1921 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     1981 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2041 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2101 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2161 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
     2221 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2281 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2341 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     2401 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     2461 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     2521 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
     2581 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
     2641 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     2701 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     2761 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     2821 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
     2881 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     2941 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3001 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3061 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3121 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3181 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3241 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
     3301 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3361 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
     3421 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
     3481 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
     3541 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     3601 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     3661 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     3721 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     3781 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     3841 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     3901 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     3961 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
     4021 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4081 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4141 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4201 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4261 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4321 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4381 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     4441 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     4501 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     4561 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     4621 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     4681 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
     4741 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     4801 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     4861 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
     4921 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
     4981 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5041 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5101 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5161 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5221 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5281 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5341 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
     5401 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     5461 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
     5521 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
     5581 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
     5641 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     5701 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     5761 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     5821 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     5881 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     5941 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
     6001 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6061 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
     6121 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6181 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
     6241 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
     6301 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6361 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
     6421 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     6481 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     6541 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
     6601 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     6661 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     6721 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     6781 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     6841 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
     6901 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     6961 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7021 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7081 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
     7141 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
     7201 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7261 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7321 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7381 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     7441 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     7501 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
     7561 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     7621 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     7681 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     7741 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     7801 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     7861 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     7921 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     7981 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8041 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
     8101 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8161 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
     8221 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8281 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     8341 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     8401 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
     8461 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
     8521 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
     8581 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     8641 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     8701 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     8761 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     8821 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     8881 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     8941 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
     9001 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9061 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9121 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9181 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9241 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9301 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9361 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
     9421 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
     9481 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
     9541 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
     9601 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
     9661 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
     9721 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
     9781 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
     9841 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
     9901 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
     9961 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    10021 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    10081 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    10141 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    10201 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    10261 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    10321 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    10381 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    10441 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    10501 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    10561 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    10621 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    10681 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    10741 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    10801 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    10861 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    10921 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    10981 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    11041 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    11101 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    11161 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    11221 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    11281 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    11341 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    11401 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    11461 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    11521 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    11581 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    11641 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    11701 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    11761 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    11821 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    11881 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    11941 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    12001 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    12061 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    12121 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    12181 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    12241 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    12301 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    12361 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    12421 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    12481 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    12541 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    12601 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    12661 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    12721 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    12781 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    12841 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    12901 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    12961 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    13021 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    13081 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    13141 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    13201 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    13261 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    13321 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    13381 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    13441 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    13501 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    13561 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    13621 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    13681 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    13741 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    13801 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    13861 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    13921 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    13981 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    14041 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    14101 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    14161 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    14221 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    14281 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    14341 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    14401 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    14461 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    14521 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    14581 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    14641 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    14701 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    14761 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    14821 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    14881 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    14941 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    15001 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    15061 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    15121 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    15181 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    15241 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    15301 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    15361 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    15421 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    15481 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    15541 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    15601 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    15661 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    15721 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    15781 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    15841 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    15901 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    15961 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    16021 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    16081 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    16141 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    16201 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    16261 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    16321 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    16381 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    16441 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    16501 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    16561 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    16621 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    16681 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    16741 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    16801 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    16861 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    16921 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    16981 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    17041 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    17101 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    17161 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    17221 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    17281 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    17341 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    17401 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    17461 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    17521 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    17581 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    17641 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    17701 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    17761 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    17821 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    17881 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    17941 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    18001 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    18061 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    18121 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    18181 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    18241 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    18301 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    18361 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    18421 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    18481 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    18541 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    18601 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    18661 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    18721 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    18781 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    18841 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    18901 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    18961 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca
    19021 gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa
    19081 taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta
    19141 gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat
    19201 cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat
    19261 gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca
    19321 acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag
    19381 tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt
    19441 cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac
    19501 gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat
    19561 tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt
    19621 ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt
    19681 gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg
    19741 gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat
    19801 ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg
    19861 ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat
    19921 ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga
    19981 catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct
    20041 tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt
    20101 ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc
    20161 ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc
    20221 aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag
    20281 gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc
    20341 caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca
    20401 tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc
    20461 actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc
    20521 ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat
    20581 ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc
    20641 ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca
    20701 tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc
    20761 gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca
    20821 acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat
    20881 cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag
    20941 ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac
    21001 ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca
    21061 tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga
    21121 taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac
    21181 ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca
    21241 tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa
    21301 atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata
    21361 acacaagatt gactaataac agaataataa atttattatc cgttacgccc ataatcaacg
    21421 aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag
    21481 ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat
    21541 cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta
    21601 tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa
    21661 tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt
    21721 tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc atgattccta
    21781 gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac
    21841 cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag
    21901 ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag
    21961 ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa
    22021 ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca
    22081 acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga
    22141 gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag
    22201 ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga
    22261 atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata
    22321 acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg
    22381 ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc
    22441 ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct
    22501 gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata
    22561 ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt
    22621 cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca
    22681 tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta
    22741 aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag
    22801 ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc
    22861 ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg
    22921 ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc
    22981 ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt
    23041 tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat
    23101 ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt
    23161 agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat
    23221 caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc
    23281 taaaagctct accattttga tagcggtttc gctttgcatc acacggatct tctttccctt
    23341 caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc
    23401 ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat
    23461 ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga
    23521 aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc
    23581 tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg
    23641 gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt
    23701 cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc
    23761 atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc
    23821 tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt
    23881 ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact
    23941 gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc
    24001 ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt
    24061 ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt
    24121 tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat
    24181 ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg
    24241 cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt
    24301 caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg
    24361 catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca
    24421 tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat
    24481 tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca
    24541 actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag
    24601 gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt
    24661 tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg
    24721 gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag
    24781 gattttttgt catctggtaa tttttataaa taccatatag accgacacct tgtggccaat
    24841 tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac
    24901 cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta
    24961 attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc
    25021 tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa
    25081 caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg
    25141 aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt
    25201 atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca
    25261 attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt
    25321 cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac
    25381 tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta
    25441 attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat
    25501 ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat
    25561 agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc
    25621 cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac
    25681 cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg
    25741 atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat
    25801 ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat
    25861 ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg
    25921 gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg
    25981 caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt
    26041 tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac
    26101 caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg
    26161 cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc
    26221 ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca
    26281 catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca
    26341 taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata
    26401 gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc
    26461 gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt
    26521 tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac
    26581 cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac
    26641 cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc
    26701 caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca
    26761 tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca
    26821 attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt
    26881 ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg
    26941 catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt
    27001 ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa
    27061 tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg
    27121 ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg
    27181 tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg
    27241 gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat
    27301 tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag
    27361 atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca
    27421 ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac
    27481 cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg
    27541 ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt
    27601 tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt
    27661 gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc
    27721 tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta
    27781 cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg
    27841 gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta
    27901 aacacttttt tgtgattttt ttcacagtta tttttaaata taaaattgct ttatttacag
    27961 gtgtttgtat agaaaaatat ataaagttat ttattattat ttatatgaca cacccgatta
    28021 ttcaatattg agcaatatat cccatgctct tatctctttc ttttttgaat aaaaaatgag
    28081 gtaggacatc tttctgccct acctcgtctt taaataagct ttgatactta tttttcttcc
    28141 ttctcttctg tctcttccag cgtttctgtt gtttcagttt ttacttcaac tgtctcagct
    28201 ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt ccctggtttg
    28261 accgttttga ttgctgcacg atcgtactca aggaagatcc cttcgcagtc taaaacaact
    28321 gtccgtttgt cattgtcgat ttctgaaaca actccatgta atccgccgat cgttacgact
    28381 gaatcacctg gtttcatgct atctaacaag ttttgacgtt cttgctgttg tttcttttgc
    28441 gaacgtgaca taaaaaacca cattgctaat agcgcgataa acatcacaag cattggtaat
    28501 gttcccattt ttttcacctc actaattctg tatctacaaa aaacacttta gcagaaaaat
    28561 gctgataaca ctagtttttt acttaatttt taaaagattt tcctttagaa gctttttgcg
    28621 ttttctttat tgaacccgta ttcttcaaaa aaggcttctc taaattcaag caaattatca
    28681 tccatgattg cttgacgtac ttgtttcatt agattcaaca agaagtataa attgtggtaa
    28741 gaagtcaaac gaatcccaaa tgtttcatca catttgatca aatggcggat ataggcacgt
    28801 gtatagtttt tacatgtata acaatcacat ttctcatcaa ttggacggaa atcatgggca
    28861 tatttcgcat ttttcactac tagacggcct tgagaagtca tacatgtgcc atttcgagcg
    28921 attcgagtag gcaagacaca gtcaaacatg tctactccac ggataacacc atcgatcaat
    28981 gaatcagcag caccgacacc catcaagtaa cgtggtttgt tttcggggat caacggtgta
    29041 gtgaattcta atacacgatt catttctgat ttaggttccc cgacagataa cccgccgatt
    29101 gagtaaccag ggaaatccat cccgactaga tctttcgcgc tttgacgacg aagatcttca
    29161 aaccctgctc cttggatgat tccaaataat ccttgacggt ctggattagc atgagctttc
    29221 aatcctcgtt ctgcccatcg agacgtacgt tctactgatt ttttcacgta gtcgtaactt
    29281 tcatcgaatg gtggacattc gtcaaaactc atcatgatat cagagcccaa tttattttgg
    29341 atgttgattg ctttttctgg tgataaaaac atacgcgaac cgttcaagtg atttttgaaa
    29401 tgcacgcctt cttcttcgat attccgcata tcacttaatg aaaaaacttg aaagccgcct
    29461 gaatctgtta aaatcggttg atcccagttc ataaatttat gcagtccgcc agcttcttct
    29521 actaagtctt caccagggcg tagccataaa tgatatgtat tacttaaaat aacgccagca
    29581 cccatttctt tcaactcttc tggggacatt gtttttactg tagctaatgt tcctactggc
    29641 atgaacattg gtgtggggaa agttccgtgt ggcgtgatca attcccctaa acgcgcgcca
    29701 gtatgttttt ctttcttgat taaacgatag cgaatggcag gttgcgtcat tcatttccct
    29761 actttctata taaaatatct tgacaactcc caccatcata atcgtttcaa gtgctttttg
    29821 caagttccct cggcttcctt ctactttatt taatattctt ttttttatcc tttttttatg
    29881 aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg gtatattggt
    29941 gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa acgccaagca
    30001 aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat ccctacattg
    30061 attggtattg ctactattct tcttattatg attcctatta tcttgtttgc caccaccata
    30121 tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag ttcaggaggc
    30181 ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat ttcttggact
    30241 tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga tgcatttcgt
    30301 ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat ctcgatcttt
    30361 acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta ttcttattct
    30421 caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc taaagtactg
    30481 gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact tttctggtta
    30541 gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat tggttatcta
    30601 tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt gccaaaagat
    30661 atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg tttatcgaga
    30721 ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca ttcatgtcac
    30781 acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt taatcattcc
    30841 tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc gattggtacg
    30901 atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag gccgaatagt
    30961 tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag taacgccatt
    31021 tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct gctgcgtaca
    31081 ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt ttgaccgaca
    31141 tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa ataataacca
    31201 aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa taaacctcga
    31261 gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg ttccgctggc
    31321 tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa cagtagttgc
    31381 acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat aaagatctcc
    31441 gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata aaccgaacgt
    31501 ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat catatctgcg
    31561 gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt acttgctttc
    31621 aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc cgtacgttgc
    31681 caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac agagagagct
    31741 ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc tgaaagtgct
    31801 tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt caccacatga
    31861 tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat tgctccttta
    31921 ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa gtctttttcc
    31981 agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc aacagcaatc
    32041 actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc tacatcgcct
    32101 gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat gtatttttcg
    32161 cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag ctcgtttact
    32221 cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac agcccttacg
    32281 atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg ctcgaccgtc
    32341 acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt cattgaatct
    32401 tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat ttcattttgt
    32461 gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt ttcaactgct
    32521 ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta tgcaagtgtc
    32581 aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga aatcgaagtc
    32641 atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac taaagccgca
    32701 gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac ctgtaaaggt
    32761 gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc tgtagccatg
    32821 ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc tttaaatacc
    32881 atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc atctttttca
    32941 actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga tgcttcgatc
    33001 aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc acccactacc
    33061 agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc cgtttgttta
    33121 ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac aatcgccaat
    33181 accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc cccatgttct
    33241 gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat cgttgtaaaa
    33301 tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc atcgaattta
    33361 ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc agtatctgga
    33421 tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc catcctattc
    33481 ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc ctttttttat
    33541 ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt taaagtgctt
    33601 atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga ggataccatc
    33661 aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt tagaagtaca
    33721 ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc gtatggatgt
    33781 ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg aataacgtaa
    33841 acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt aacgaaaaca
    33901 aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga tggaaagtta
    33961 caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct tatcgagagc
    34021 tcggcttttt ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc
    34081 ttttcataac cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct
    34141 tcaatcgcat gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac
    34201 atgactaaag tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat
    34261 tcatagccag gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta
    34321 aatttcgttc caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc
    34381 ccgccatttt tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat
    34441 tcactatgca tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac
    34501 cctacatgta gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat
    34561 tctttcgtaa aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag
    34621 acagtttgat agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt
    34681 tctcccaatg attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt
    34741 ccgccgtgtt ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt
    34801 cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt
    34861 gtattcgtta gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa
    34921 cgagcaggca gaactcgtgt attattcata actagcgcat caccaggctg taactcttca
    34981 atgatttcat ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa
    35041 cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa
    35101 tcaaaatctt ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct
    35161 atcatgtttt tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc
    35221 taaataaaaa aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat
    35281 agttcttcat tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa
    35341 aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg
    35401 agttcatttg ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca
    35461 accgaatgac tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa
    35521 ttgatccagt ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc
    35581 ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag
    35641 aagaacggaa taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt
    35701 tagatcgcta cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg
    35761 cccttgcagc gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa
    35821 tcactcgtga ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga
    35881 tcgtatttgt aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca
    35941 tgagtgaagg ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa
    36001 acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga
    36061 caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac
    36121 aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt
    36181 cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat
    36241 ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc
    36301 gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac
    36361 agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga
    36421 tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta
    36481 aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag
    36541 gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac
    36601 cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca
    36661 gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg
    36721 taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg attttacgta
    36781 atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg
    36841 ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca
    36901 tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata
    36961 tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc
    37021 tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag
    37081 gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg
    37141 tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc
    37201 tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga
    37261 agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac
    37321 cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga
    37381 tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc
    37441 tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata
    37501 cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc ttgttcgaaa
    37561 agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga
    37621 attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt
    37681 ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta
    37741 tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat
    37801 cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc
    37861 tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa
    37921 taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa
    37981 taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga
    38041 agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag
    38101 gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt
    38161 gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa
    38221 ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg
    38281 atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt
    38341 ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa
    38401 aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta
    38461 gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc
    38521 atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc
    38581 cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca
    38641 ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc
    38701 agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt
    38761 cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc
    38821 cttatgggta aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat
    38881 actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca
    38941 ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga tctctaaata
    39001 ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat
    39061 ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg
    39121 tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat
    39181 ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct
    39241 aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg
    39301 atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa
    39361 gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat
    39421 ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt
    39481 cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc
    39541 ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt
    39601 gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt
    39661 cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc
    39721 tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca
    39781 acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta
    39841 aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt
    39901 tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt
    39961 gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga
    40021 tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa
    40081 gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc
    40141 tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact
    40201 gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt
    40261 cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt
    40321 cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac
    40381 taataatctc gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt
    40441 tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata
    40501 taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc
    40561 aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca
    40621 cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac
    40681 gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac
    40741 acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca
    40801 cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca
    40861 aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt
    40921 agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat
    40981 ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg
    41041 taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt
    41101 atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc
    41161 atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa
    41221 acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt
    41281 tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc
    41341 aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca
    41401 aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata
    41461 tctacataac ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa
    41521 cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct
    41581 tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac
    41641 gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa
    41701 tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca
    41761 tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc
    41821 cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata
    41881 ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct
    41941 gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct
    42001 gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca
    42061 ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt
    42121 gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa
    42181 atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta
    42241 ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata
    42301 aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg
    42361 gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca
    42421 gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc
    42481 acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat
    42541 aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg
    42601 aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc
    42661 acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca
    42721 cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa
    42781 atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga
    42841 ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca
    42901 aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca
    42961 acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa
    43021 gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact
    43081 aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt
    43141 ttatagtcga aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc
    43201 aataaacttt aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg
    43261 ctttaataca gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa
    43321 aaggatagaa cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga
    43381 agagagatgt aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg
    43441 tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa
    43501 attaaaaaca taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct
    43561 tttttacaac ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa
    43621 aacggcaaaa tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac
    43681 tgatttcaca acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat
    43741 ttcgtcattt tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca
    43801 aacaaaccga tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca
    43861 gattcccgta acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag
    43921 aatttccctg agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag
    43981 atgataatag cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc
    44041 atcatgaacg ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca
    44101 tctactccta gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt
    44161 gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct
    44221 ttctttccta aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac
    44281 agacatagtg cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc
    44341 gcattggttt gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt
    44401 ttcaataatt tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct
    44461 gctgcttttt ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca
    44521 gcgttcacag ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg
    44581 atttcatttt ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt
    44641 tgctgaatga tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg
    44701 atggtatcca tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca
    44761 ggcatttctt tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt
    44821 gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc
    44881 ccaatcgtat cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta
    44941 ttgataccag caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat
    45001 tcatcaatag ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt
    45061 tgcagattgc ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc
    45121 gtatttccag cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt
    45181 tgttgttgag atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca
    45241 tcatcagtca gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc
    45301 gttgttgcca cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt
    45361 atcgcatctt tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga
    45421 ttttctaaat ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct
    45481 tgttttgctt catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg
    45541 atctctgcta gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg
    45601 atgatttttg cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca
    45661 cccatctcat ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc
    45721 ttgatttcag gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg
    45781 tccaaatttt catctgttga taaaatcata tctttaactt tattgatact tactaggttc
    45841 gtatcgatgt cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg
    45901 ataaattcat ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt
    45961 gcaatcgcat tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca
    46021 ctagtaaaac tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac
    46081 tttccggaac ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat
    46141 tgtttatttt tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga
    46201 aactctgctg gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat
    46261 ggatcccata atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg
    46321 atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat
    46381 cgaagcgtat ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta
    46441 ctttaccatg tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg
    46501 acatatcacg gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta
    46561 cagcctttac tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct
    46621 attatacgta tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt
    46681 cagctttttt cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc
    46741 cagcaggagg atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag
    46801 acagattcat tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag
    46861 caaaggcttc tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct
    46921 ttttttgccg caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt
    46981 tcatgatctg gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt
    47041 ctgcctgatc agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag
    47101 ctcctaattt ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc
    47161 ccttttcatg gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca
    47221 ttacagtatc tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc
    47281 ctggcgtgac cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt
    47341 ttgctgatgg caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc
    47401 gatcacctac atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat
    47461 cattacctgg tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca
    47521 aggcaacatc ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac
    47581 cgacttctct gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt
    47641 taattgcaaa tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct
    47701 ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt
    47761 cttttttcgc gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct
    47821 aataaaaaat aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa
    47881 aaaacagcaa gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa
    47941 agattacgaa ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg
    48001 tgatttaatc gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa
    48061 attccgtgaa ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc
    48121 caagtctggc tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa
    48181 agatacgttc gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta
    48241 cgcacagctg gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta
    48301 gtaacaggag tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc
    48361 ataatgggtc gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg
    48421 atctgctcca acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca
    48481 atgtgagtcg gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc
    48541 gggctaaaat ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc
    48601 tccgatccca cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga
    48661 tcctccttat tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata
    48721 gtggatcgta cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt
    48781 gccgataagc gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg
    48841 atccatacat acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc
    48901 ctgcatcttt cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc
    48961 ttcaggagtg atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac
    49021 ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc
    49081 gtgcccagag ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt
    49141 ttcatcatgc cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg
    49201 aagttttttc agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc
    49261 ctgatctgct gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt
    49321 gatgaatgca gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc
    49381 agtgtctgct tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc
    49441 agctgctgct tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc
    49501 tacagtcagt gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc
    49561 ttctctcaac tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc
    49621 aactgctgcc tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc
    49681 aaacaattct tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc
    49741 ttttttatgt atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc
    49801 tttctagtct tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac
    49861 ccatacattg ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa
    49921 aatctcgaca tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc
    49981 ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg
    50041 gaatagtttc atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat
    50101 cagccgagtg atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt
    50161 ttctcttttc aataaattta gaacactctc attctaccaa attataacca ttttgccaca
    50221 aataagtcca caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta
    50281 tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc
    50341 gccatctttt tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata
    50401 attaccgccg tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta
    50461 tccttttttc tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc
    50521 tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc
    50581 taataattta tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc
    50641 gatttgttca actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg
    50701 taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt
    50761 tggtgtaacg aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg
    50821 gtgaacgtag ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga
    50881 aatatctaat ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt
    50941 aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc
    51001 acggtaaccg cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc
    51061 gaagacgatt gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt
    51121 ttcgtattct tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc
    51181 tttgatcttc acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg
    51241 cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt
    51301 ttcgatatct tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt
    51361 ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt
    51421 acccacaacg atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc
    51481 gccgtaaacg gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg
    51541 tgtttggata gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc
    51601 tggatcgatt ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg
    51661 tgcttgtcca ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg
    51721 cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag
    51781 ttctttaaat ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg
    51841 ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg
    51901 tttttatcca ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca
    51961 ttagaaaagc caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca
    52021 agttgtttct atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct
    52081 aacatagttt ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc
    52141 taccacttca aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc
    52201 aagattttgt ttgatagcat caatcaattt attcttttct ttttcaaaat agaacacagg
    52261 tagttggggg tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag
    52321 gaaaatcatg gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa
    52381 agactctttc cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg
    52441 cagattttta aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc
    52501 gctcaaaata gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga
    52561 aacatgggcg agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt
    52621 atacgcaatc aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc
    52681 tccttctact acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc
    52741 gccatttcca ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag
    52801 caacggttca tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc
    52861 cattttggct ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat
    52921 cattgtgatt gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc
    52981 gtgatccatg cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt
    53041 gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc
    53101 agacaagaca gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc
    53161 aacgacttct gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa
    53221 aggatctgct acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca
    53281 aaatgaagca gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt
    53341 accagcaaga ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt
    53401 ggtaatcttt gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt
    53461 tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg
    53521 ggttcgcatg actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga
    53581 acgcggttac gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa
    53641 aaagattgac gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca
    53701 ccgtatttca agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct
    53761 ggcatagagt ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac
    53821 cggctcaatc cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt
    53881 tgataaactt cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat
    53941 tttgcttcat aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga
    54001 acgtcttcat ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg
    54061 attgctcgag aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt
    54121 tctgcttttg taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa
    54181 agcaaagaac cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta
    54241 agtactggta cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg
    54301 attttatcca tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat
    54361 gtctctaaga agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa
    54421 acgatggctc cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc
    54481 tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct
    54541 gttagtaatg gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg
    54601 taataattat aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc
    54661 tctgcgctgc gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta
    54721 aatcgttcag tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat
    54781 ttataaaaaa acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga
    54841 ttatttgtcc cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc
    54901 aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag
    54961 tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag
    55021 gccccacctt catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg
    55081 ccttcactca aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg
    55141 tcaaaaagac gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt
    55201 caaaatcgcc ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg
    55261 gtgacgcggt agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc
    55321 ttcaactatt tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg
    55381 atctaaaagg ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa
    55441 aagaaagtca aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca
    55501 gccccaagat ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg
    55561 gtaaaaactt gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa
    55621 ttcaaaacaa gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac
    55681 aggtgtttga tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg
    55741 tactaacaaa agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag
    55801 agttcggttc ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata
    55861 ccatcaacta catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac
    55921 tcacacaagc cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc
    55981 ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa
    56041 gttttcttga acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca
    56101 cacatgtcgt gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt
    56161 tgctgaaagc atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc
    56221 tcatggaagt cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag
    56281 atacattgaa acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc
    56341 ctcaacagct ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg
    56401 aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt
    56461 tcggtatgat tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg
    56521 tagaaaacgc agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg
    56581 tcattatttt tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga
    56641 aaaaggttgt gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt
    56701 ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga
    56761 gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac
    56821 atggaacaca tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa
    56881 taccttcgtt acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac
    56941 gcttgacgaa aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg
    57001 ccagcttttc ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct
    57061 tcgtcaaatt cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa
    57121 gctaaaccat aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta
    57181 taatccgcac tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct
    57241 ttggtcgtat aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct
    57301 aattttatga catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt
    57361 tttcgccttc ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt
    57421 gatccatttt gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag
    57481 cgtgatcaat aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt
    57541 catcaataaa aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat
    57601 ccggcgatcc attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc
    57661 tgttactacg tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa
    57721 gaggaccaat aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa
    57781 acgtttcaaa atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa
    57841 atacataaaa atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa
    57901 taaaaaaagg aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg
    57961 caacttccat tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa
    58021 gaaaccataa atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa
    58081 aagaaacaat aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt
    58141 tcttttgatg tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc
    58201 catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa
    58261 ctcaatcgct tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc
    58321 catattgata aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag
    58381 ccatgctgca ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac
    58441 gcctaatatt ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt
    58501 gtaactatct agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc
    58561 ataaaacagc gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa
    58621 taaaagataa ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa
    58681 attacgccca aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata
    58741 actaatctgc tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat
    58801 tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg
    58861 attactcaag cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc
    58921 aggtgataaa gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa
    58981 accagagtcc ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc
    59041 gagagctttt ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag
    59101 ttcttctgta atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg
    59161 attcactcgc aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg
    59221 aaaaactgaa gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa
    59281 ataaattttt ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt
    59341 taataaaaag taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata
    59401 agttttcagc atgtaatcca cttgtttttg accaatgatc ggcacaaaaa cttccggcca
    59461 tacttcttgt atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc
    59521 catacaccca cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag
    59581 catgatcatg ctatacacac ccaccgacca cggtttcaag aaaatcacaa tctgcataaa
    59641 ttccctaaaa gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa
    59701 gggaagcagc aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa
    59761 ttcatcaaat ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata
    59821 gtaacggaca agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa
    59881 aagcccaaat ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa
    59941 aataggaatg atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc
    60001 aaattgctgg ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc
    60061 ataaagaatc aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt
    60121 ttgtaaaact gatccttttt tagacatcta tattccccac atcttttttc atttattaaa
    60181 actgcctcct
//
LOCUS       DARBFD010000007        53984 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000007 DARBFD010000000
VERSION     DARBFD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.007"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(15..1379)
                     /locus_tag="SCK08_000430"
     CDS             complement(15..1379)
                     /locus_tag="SCK08_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1200348.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1376..2935)
                     /locus_tag="SCK08_000431"
     CDS             complement(1376..2935)
                     /locus_tag="SCK08_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1200349.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2991..5471)
                     /locus_tag="SCK08_000432"
     CDS             complement(2991..5471)
                     /locus_tag="SCK08_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200350.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5455..5790)
                     /locus_tag="SCK08_000433"
     CDS             complement(5455..5790)
                     /locus_tag="SCK08_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200351.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5804..6838)
                     /locus_tag="SCK08_000434"
     CDS             complement(5804..6838)
                     /locus_tag="SCK08_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1200352.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6928..7608)
                     /locus_tag="SCK08_000435"
     CDS             complement(6928..7608)
                     /locus_tag="SCK08_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1200353.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7892..8995)
                     /locus_tag="SCK08_000436"
     CDS             complement(7892..8995)
                     /locus_tag="SCK08_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1200354.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9624..10094)
                     /locus_tag="SCK08_000437"
     CDS             complement(9624..10094)
                     /locus_tag="SCK08_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEG1200355.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            10424..12064
                     /locus_tag="SCK08_000438"
     CDS             10424..12064
                     /locus_tag="SCK08_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEG1200356.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            12218..13540
                     /locus_tag="SCK08_000439"
     CDS             12218..13540
                     /locus_tag="SCK08_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEG1200357.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13608..14042)
                     /locus_tag="SCK08_000440"
     CDS             complement(13608..14042)
                     /locus_tag="SCK08_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1200358.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(14107..14944)
                     /locus_tag="SCK08_000441"
                     /pseudo
     CDS             complement(14107..14944)
                     /locus_tag="SCK08_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(15686..16312)
                     /locus_tag="SCK08_000442"
     CDS             complement(15686..16312)
                     /locus_tag="SCK08_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200359.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(16607..16942)
                     /locus_tag="SCK08_000443"
     CDS             complement(16607..16942)
                     /locus_tag="SCK08_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG1200360.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(16929..17213)
                     /locus_tag="SCK08_000444"
     CDS             complement(16929..17213)
                     /locus_tag="SCK08_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG1200361.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(17269..18792)
                     /locus_tag="SCK08_000445"
     CDS             complement(17269..18792)
                     /locus_tag="SCK08_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1200362.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(18785..19960)
                     /locus_tag="SCK08_000446"
     CDS             complement(18785..19960)
                     /locus_tag="SCK08_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1200363.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(19964..20149)
                     /locus_tag="SCK08_000447"
     CDS             complement(19964..20149)
                     /locus_tag="SCK08_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200364.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(20115..21809)
                     /locus_tag="SCK08_000448"
     CDS             complement(20115..21809)
                     /locus_tag="SCK08_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1200365.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(21806..22279)
                     /locus_tag="SCK08_000449"
     CDS             complement(21806..22279)
                     /locus_tag="SCK08_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEG1200366.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(22348..22503)
                     /locus_tag="SCK08_000450"
     CDS             complement(22348..22503)
                     /locus_tag="SCK08_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEG1200367.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(22637..23017)
                     /locus_tag="SCK08_000451"
     CDS             complement(22637..23017)
                     /locus_tag="SCK08_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1200368.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(23021..23236)
                     /locus_tag="SCK08_000452"
     CDS             complement(23021..23236)
                     /locus_tag="SCK08_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200369.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(23239..23646)
                     /locus_tag="SCK08_000453"
     CDS             complement(23239..23646)
                     /locus_tag="SCK08_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEG1200370.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(23912..25387)
                     /locus_tag="SCK08_000454"
     CDS             complement(23912..25387)
                     /locus_tag="SCK08_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEG1200371.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(25377..26225)
                     /locus_tag="SCK08_000455"
     CDS             complement(25377..26225)
                     /locus_tag="SCK08_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEG1200372.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(26262..26624)
                     /locus_tag="SCK08_000456"
     CDS             complement(26262..26624)
                     /locus_tag="SCK08_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200373.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(26625..26783)
                     /locus_tag="SCK08_000457"
     CDS             complement(26625..26783)
                     /locus_tag="SCK08_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200374.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(26924..27548)
                     /locus_tag="SCK08_000458"
                     /pseudo
     CDS             complement(26924..27548)
                     /locus_tag="SCK08_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(27545..27856)
                     /locus_tag="SCK08_000459"
     CDS             complement(27545..27856)
                     /locus_tag="SCK08_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200375.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(27900..28193)
                     /locus_tag="SCK08_000460"
     CDS             complement(27900..28193)
                     /locus_tag="SCK08_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200376.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            28387..29034
                     /locus_tag="SCK08_000461"
     CDS             28387..29034
                     /locus_tag="SCK08_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1200377.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            29095..30240
                     /locus_tag="SCK08_000462"
     CDS             29095..30240
                     /locus_tag="SCK08_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1200378.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(30321..30626)
                     /locus_tag="SCK08_000463"
     CDS             complement(30321..30626)
                     /locus_tag="SCK08_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEG1200379.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(30651..31082)
                     /gene="ruvX"
                     /locus_tag="SCK08_000464"
     CDS             complement(30651..31082)
                     /gene="ruvX"
                     /locus_tag="SCK08_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEG1200380.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(31079..31345)
                     /locus_tag="SCK08_000465"
     CDS             complement(31079..31345)
                     /locus_tag="SCK08_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEG1200381.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(31600..32325)
                     /locus_tag="SCK08_000466"
     CDS             complement(31600..32325)
                     /locus_tag="SCK08_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG1200382.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(32686..32756)
                     /locus_tag="SCK08_000467"
     tRNA            complement(32686..32756)
                     /locus_tag="SCK08_000467"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32722..32724),aa:Gly,seq:ccc)
     gene            32873..33625
                     /locus_tag="SCK08_000468"
     CDS             32873..33625
                     /locus_tag="SCK08_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1200383.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            33641..34120
                     /locus_tag="SCK08_000469"
     CDS             33641..34120
                     /locus_tag="SCK08_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1200384.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(34371..35951)
                     /locus_tag="SCK08_000470"
     CDS             complement(34371..35951)
                     /locus_tag="SCK08_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEG1200385.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(35944..36687)
                     /locus_tag="SCK08_000471"
     CDS             complement(35944..36687)
                     /locus_tag="SCK08_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1200386.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(36863..37639)
                     /locus_tag="SCK08_000472"
     CDS             complement(36863..37639)
                     /locus_tag="SCK08_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1200387.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(37887..38723)
                     /locus_tag="SCK08_000473"
     CDS             complement(37887..38723)
                     /locus_tag="SCK08_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEG1200388.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(38734..39663)
                     /locus_tag="SCK08_000474"
     CDS             complement(38734..39663)
                     /locus_tag="SCK08_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEG1200389.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            39855..40208
                     /locus_tag="SCK08_000475"
     CDS             39855..40208
                     /locus_tag="SCK08_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1200390.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(40282..40938)
                     /locus_tag="SCK08_000476"
     CDS             complement(40282..40938)
                     /locus_tag="SCK08_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1200391.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            41164..43098
                     /locus_tag="SCK08_000477"
     CDS             41164..43098
                     /locus_tag="SCK08_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1200392.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(43184..44407)
                     /locus_tag="SCK08_000478"
     CDS             complement(43184..44407)
                     /locus_tag="SCK08_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1200393.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(44401..46299)
                     /locus_tag="SCK08_000479"
     CDS             complement(44401..46299)
                     /locus_tag="SCK08_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEG1200394.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(46293..47411)
                     /locus_tag="SCK08_000480"
     CDS             complement(46293..47411)
                     /locus_tag="SCK08_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1200395.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(47494..48327)
                     /locus_tag="SCK08_000481"
     CDS             complement(47494..48327)
                     /locus_tag="SCK08_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1200396.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(48320..49237)
                     /locus_tag="SCK08_000482"
     CDS             complement(48320..49237)
                     /locus_tag="SCK08_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1200397.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(49464..50588)
                     /locus_tag="SCK08_000483"
     CDS             complement(49464..50588)
                     /locus_tag="SCK08_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1200398.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            50771..51679
                     /locus_tag="SCK08_000484"
     CDS             50771..51679
                     /locus_tag="SCK08_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1200399.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            51820..53433
                     /locus_tag="SCK08_000485"
     CDS             51820..53433
                     /locus_tag="SCK08_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1200400.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        16174 a        10804 c         9112 g        17894 t
ORIGIN      
        1 gaccgtgata tttctcacag tcttttatct actctattgc tatttgctct cttagctagt
       61 actttaaaag ctaactcatt gaggtagtga aaacgagagg tgtgttcaga cagaaatcca
      121 aaatcctcaa ctgctagctg tggaatcgta gttttgtgtt tcacgatatc cgcaagcagg
      181 ttcaaatctg ataataagaa ttcttggctt atcagctgtc cgccaattat ttctaatgta
      241 tccggatggt agatcagctt tgcatttact gttttgtttt cttcatcgta ttgtggaaat
      301 agatcaaatt ctcctgaaca cgaaccggta ttttgttcca agaatggcgc ttcatcttct
      361 gtcagcccta cagttcctaa aaagtagtct ataatgactg ctgctgttat cttttgtctg
      421 aggtcgtaag cggttggctg accactcaaa gttagcgcta ctgctcttgc catatgtaca
      481 gctcgagtaa ctaaaggtaa aaagagatgc cgtttatttt tgtcaaaact gactgggacg
      541 atatctccaa tcgcatagat gtccggatca cttgtctgca gatattcatt gactatcaca
      601 gtctcatcct catacaaagc cactttttct tttaacaatg tcgtattagg acgagtttgg
      661 gcagaaaaaa tgaccatatc tgcctttatt gcttgagata gaagttgtac ggatactact
      721 ttctcttctg attttttcca atcgatcaag aattcatttg gataaaaatt gatttgctta
      781 gtttgcagct gattttgaaa actgttgatc atatccttat cgaaatatct tgataaaaca
      841 ttcggcatat aatcaactag atgaataatg tatcctttat ttctcaaagc atctgctaat
      901 tccaatccaa tataaccagc accgaagatc actacttctt ttgcttcttt taaagcggca
      961 agtacttgct tagattgcac tagattttta taggtgtaga cattttcagc ttcaatttgt
     1021 ttatacaagg tgacaggaga ggaacctgtt gctacgatta atttatcata ggttatggtc
     1081 tcgttttttc cttctttgga tgcgaggatg agctgatgtt tgtctggatc aagttcagtt
     1141 actctccatt cggtgatcag ttttgcccca ctttcttgaa gagtccgcat gttatgggca
     1201 acttcagcta aactacgtat ctttcctttt aaataaaggt tgattccatt tgaaacatag
     1261 cctagtccat ctttatgatc ttcatcgatc agtaaaactt ctgctttcgg ttgaagtttt
     1321 cttagattta gggcagctgt gatgcctgca tgggaagcac caatgatcac gattttcatg
     1381 ttttattcat ccttaaattc aatttttcta aataacattc ttttaaaaat tggttcaaat
     1441 aggggaaatc ataattatat tcgttgctag tgaatacaaa aactttagcg gacttctgtg
     1501 acgggtactc tttcaattgg ctagtcaaaa tgatatcata ctgcttttct ttctcgtatt
     1561 cgactaaatc atatttgatc gtattcagca attgttcctg gatgatttct ttcacgatat
     1621 ccgccattag cctttcataa ggaagatcat aagcaatttg cagacgtaat tctaactgct
     1681 tgatcagcgt ccgcaaaata gatgcatagc gagcaaataa ttcaaagcgg gcattttcct
     1741 cgtctacttc agtaaaatca acaatagcaa atgtttgtct gaccaatctt tcacataaaa
     1801 ccaggaattg tttatcttct acttcagtta accgctgttt taatccttct tggccaaaaa
     1861 tattcatcca tcctctaaaa taggcaaaat gagcgtgatt ttgcatcact gtcaacgcaa
     1921 tcttttgcga aatctcttct ggaaaaacat aaaaagagaa atattcttct atcgattgtt
     1981 taaacgttcg attcaattga gctactacgt cgtctttgta tttttcgctt atcaaaaact
     2041 cctggacatg cgagttatgt gtatctaatg taaagttact gatcatgaaa agataaaaaa
     2101 ccaacgtttc attttgattc caaggaaagg catattgaaa gaagtaacgg gtcaaaaatg
     2161 tatggaggtc caataaaaat tgtgtcgttt gaaacaatcc aacaggaaga ttgctataat
     2221 ctggtttgga ttttttcgta cagcgcattc gcttcttcat aatggaaagc catatttcaa
     2281 atttcaatcg tccttcttct gtaatagtaa aactaaactc tttttgcagc agatctaaaa
     2341 aagtgtttcc tgataaatca ctgtattctt ttttgttttc aagataagga cgtccaaacc
     2401 aaaataattg gaaataaaaa taacaaattt gactttctgg accggtcagc cgcccgcttt
     2461 ttattttcag atgaaactca tttaaagaat catttaattc cttcactctg cgagacaaag
     2521 tagccgaact gatattaagt tccatgacaa gtttctgccg atcaaatgag ccattttcca
     2581 acagataaag aagtatctga tattttgtag aatagcgcaa gtagcaatga taataaacat
     2641 cgactgctgc tatatttcca tgtctgatta ataaaatatt cccttcttgg ataacaattt
     2701 ccaattgttt gatattttcc aagttagctt gtaaataaaa cagatcttct ttcaaggtag
     2761 cccgatcaac tgataagcgg gtaagtacgt tattaacgga aatctctcct ttttcctcta
     2821 ataaaagttg gaaaagtttg atttgccgtt gttcattttt ttcaagcaaa ctgaaaagtt
     2881 ccaagtccac tcctccaagc atttctgatt ttaacagcag aagtttcctt ctcatccttg
     2941 aaaaagagga tctgtgacag ttatcacaga tccatacaaa ttctaatcac ttagctggtt
     3001 ggcccagctg tcagttccca tgtgaccatg ccttgatacg ttgcgccagg ttgagcattc
     3061 gtactgctat ttacctctaa gagtaaacca tcggtatcgg gaaagtcaac gagaacctct
     3121 cctttagctg ttccatcact ctcataaatc aacgtgtttt gaccttgctg aatcaattgt
     3181 tttgatccag aatgattgaa gtataatttt gttccgctta attcttgacc ttgatcattc
     3241 ttaaataaag aagtcaatgt ccctgtcagt tgccaaggag acgtgcttgg aagccgagta
     3301 tcccgcacat caaaagaaaa atcgcctttt gcttcataat aacttgtctt caacggtttg
     3361 gggagatcgt taccaaatgt aaaatcatct ggtacattaa ctaatgttaa ttcttgtttg
     3421 gggacaagaa ttgttttagc agatgtttgg acaactgggt taggaagctt agcaccatct
     3481 gctaatgtaa tattgctgga aagtacagct gcctttgtca gtatgctgac ggattgtaca
     3541 gaagtatcta cagtatttaa ttgatagttg taagtaagat gaatggtatc tcctattttg
     3601 ctcaatagat ctgctgggac agaaactcct tgttgaatgc cggtatctgc gatagttgta
     3661 ccatttaatt ttaaggaacc attgatataa gtagtatttt caggaagtga gatcgtgatt
     3721 tttttgtcgg ttatcgcatc cccagatttt gttaacgtga atgtcacatt ttcttgaatc
     3781 gtatctcctg atagagcaga caggggatct cctgtctcag gttgtctgct aaggtttttt
     3841 agctgactat ctgcactcac agatggaaaa ttagccactg ttatttgagg aagagtggtg
     3901 attccagctt gttcagactg ccatttgtac gcaaaagacg gatacatgtt agtaagtaca
     3961 tcgtccacca agcgataatc aaaataatat tgccctgaag ccaatgcagg tagtgtagaa
     4021 gtagctgtat ttacaccccc gttatttgat tggatcgttt ggaaaagttg atctgtccat
     4081 cctgtcgcta atttttgggt tcctttcgaa taaagcctgt aatggatcga accctttttt
     4141 agttcagttg ctgaccaatt ggccgtgact tggtacgatg cagaagaaag gagctttccc
     4201 cactgactat cggctgttaa gtttgttggt aaaaccgttc cgtctagctt gtactgctcc
     4261 aataccttta aagcaacagg agaaagcaat ccgccttgcg catctttccc actgtacacg
     4321 atattggctg tctgattcaa tccacttgtg cttgctggag tatgagtcag tattgctcct
     4381 tgtgtaacgt tttttcctgt aaaatagttg actacgcgat caaagcgact aaatggcaac
     4441 tgatcaaaac gtccttcagt cagccccatc gtcaatgtcc gactattggt agagcctgga
     4501 aggttctggt tatttccatc cacgtattct gtggtatcag caaatgtatt atattttcct
     4561 gttgaatcac ttgttgagat gtaactattc cacgtattta tctctgcact tgcagaaaat
     4621 gcatatgctc ctggattcaa gtttccgcca gttaatttag gataaaatct acctgcagct
     4681 ggagctaaaa tcgaattgaa catacgatct tggataatat tattcgaatc ataaaaatca
     4741 acagaaaaaa cggccttttt agacatagaa ggttgtaatt tacccttggt caaaattttg
     4801 tttggactcg tatcatattt atctccgttt tctgtcatct tcgcgacatt agctgtaaca
     4861 tcaaattgaa gcgggatatc gtccacgttg aattcgcctc gcgtaataat agcaataata
     4921 tctgccaata gagtatttgt atcccacgac cccatcccaa aacgcaaggt atggcttcct
     4981 gctgataata aactaccgct agtaaccttg atgggggcac cggcaatata caagtaggcg
     5041 ttgttccagt caattgcttt taatacatca ctgcttgata catgttccgg caaattgatg
     5101 atcgccacaa atgcaaatcc ttgtgcagtg atattcacca aatcaagtac atttgttagt
     5161 ttcacatggc tttcccaatg aaaacggacc gtgccacctt tccaaggatc atttgaacca
     5221 tcgttcaaat aagtagcagt caatttaggc aattggttag ccccagttag ttggacaagc
     5281 ttcccgccat tgactgcact acgtccacca tttggaggta cgatattttc atacgtgtat
     5341 ggatcataag aaatggcagg aggcgttgct ccaacagctt ctgccgaagg tgaaaagata
     5401 aatatactta aaacaaaaag cgaagtaaaa aagaaggagc gaaaaaatga aaatttattt
     5461 tgtagcaaca tgttcatcct ccttttcctt tcttctccat aacaacaacc acgcaagaag
     5521 ccataattct ataccaatga tcaaataaag aaaacggatc ttttcttcat tcgtctccgg
     5581 gaatacgcca tgttgattag attctttata aatcggtagc tgtgtctgct gttttgatgc
     5641 gccattgact gaaccacttg gatgcggaac tggacttaga tcataaaatg taatcgtacc
     5701 tgaacttttg gtatctgctt ggactggctc ttgccagctg aagaacagca aacttgcaat
     5761 aatccctccc aataagatcg atgttttcaa cgaaatcacc tcatcatgtt gtttctcctt
     5821 ttttcttttt cttgtggacc cacaaaaaca gcaaaatcaa ccataacact agaactgcag
     5881 caaccaatcc tgcataaagc catgggaaag attgttcttg tactgcaaaa acggaatttt
     5941 catttacttt ttgtgctttt tcttttttga tcgtaaagtt ttgtgtaaat cgccattcat
     6001 tactgtcgcc tgattttgct atgatttctg cgcggtaatc gccggctttt gctttatcac
     6061 cagctaaatc aagattcaag tgaaaattcg aatttggtgc aactaaaaat tcattagacg
     6121 tattttcaat ataaagctga ttttcataaa agatcttgct cgtaaatatc aaattgctga
     6181 taatcctagg ctgaggattt tggaaacgaa gctgaacagt tggctttgcg ttgacctcgt
     6241 ccaaatctcc tgataaaagt ttcagatccg gttctatcgt ctcttgactt tcttgtaaaa
     6301 cgactgcgag attataagca atacgatttt tgatttgtgc tccttctgta gcattttctg
     6361 tttcagcttc ttctttaggt gaaacatgaa tacccgctaa tacacgtccc ttaaagcttt
     6421 ttgctgggat tttcacttct ataggtatag ttgtttctgc atgtgctgat acattgattt
     6481 cctttttcgc aatgagcaaa acgtcacgga catcaaacgg caatgtctca tctaacgttg
     6541 ggtttttttg actataatcg atcgttccac catcactcgt taccgccgga ttgacagaga
     6601 cctcaaatgt tcgttcttca tctgaagaat tgctgataat tacttctagt gtctgatttt
     6661 gttccggtgc cactaataaa tcaaaataac cgattgcttt atctatttgg ttctctggaa
     6721 ataccggttt gattccaaag tctcctgctg aatcctttgc ttggctgatt gacggaaaaa
     6781 tgaaaaatag acttaaaata gaaaaaaacg aaacaaacag tcgtttactt tgattcattg
     6841 tccaccacat cctttcaaca acaactgcgg taaaaaatcc ttcacctata gtattttcac
     6901 cgcagtttgt tgaatttatt tcatactcta tgctactggg ccagccacga ggttccaagt
     6961 caaatcagcc gtgaatgttc cagctgctgc tttcggcact ttcagtgtga tatttgctgg
     7021 atcataaaca gaaagccatg ttcccagacc ttgatctttt gctgctgtaa atattggtgt
     7081 cgctgcactt gctttactca acccagttac agtttgacta gttggtccag tggattcgtt
     7141 atctgacgta cttttaacag tggatgttcc aaaatctaat tcagcgcctt gcaaaacttg
     7201 tgaaccgttt acaaaatctg aaaccgaaac tgtcacgttc cacccttgtg cttggccatc
     7261 tgcacctaca cctcgacgat ctgatacttg gagataaggt gtatcgtttt ttgaagcgtc
     7321 tacttgatac gtttttgttc cgctttcaat ctcgtgtgta ccaaatggca attcaggaac
     7381 tacatccaat gttaaagaac ctgtctggcc agttgctgga ttttccggat ctgctggctc
     7441 tactggtttt gaaggatcag tagggttaag aggctcaacg gtatttgttg gttcagtaaa
     7501 ggtaatatct ccttttgaag tcgcttgacc ctcagcaaaa gctggtgttg ccaagccgcc
     7561 tagaagaagt gttgaaagta ataatgtccc aatcatgttc tttttcataa taaatcactc
     7621 ctttttctta gtgacctaag tatagtagag acagtatgca aaaaatcctc atgaattcat
     7681 ccgcttactt gaaattatga ccctttttgt ataaaagaga aaaaaacaat aaaatcgttt
     7741 acatgaatat tcttcctttt ttattaagta tgaaaaaata tttcaatttt attttttcgt
     7801 ttcaaattat tctattagtg acatccttga actataaaaa taggagtacg gcatcatctg
     7861 ccgcactcct atttttttcg tcatttattt cttaaataaa acgattctca cgcaatttta
     7921 tttttttgat atcttctact gtttgtgttc cagctaattg catgaccatt tccaattctg
     7981 ttttaaagaa atcaaacact tgatggactc ctgtacttcc tcctaaagac aatccataaa
     8041 taacgggtct gccaatagca acaagatcgg cacctgatgc gattgctttg aaaacatgtt
     8101 gtccacgtcg tacaccgctg tcaaacacga ttggtacacg tttatctact gcttctgcca
     8161 catattgcaa cgaatcaaac gcagcaggcc ccccatctaa ttgacggcca ccatggttag
     8221 atacccaaat tccaccagca ccagattcta atgatcgata gacatcttct tctgattgaa
     8281 cacctttcac gtagactggc aaatcagaat gagcagcgat aaattctaca tcttttggac
     8341 taagtttttg tttggaagat ttatatacag catccatcgt ttggcctact cctgattgat
     8401 aagcctgaac gatcggcatt ggcagcggga aggtaaaccc gtttctgcgg tctgtttcac
     8461 ggtttccgcc aactgttgca tccgctgtca atacgattgc ttttgctcca ttacgcttgg
     8521 ccacttcaag aatatccaaa ttgattccat tatctttgct catataaaat tgaaaccatt
     8581 gcggtgcttt ctccccgcct gcttctctta tctcttctaa agtacaagaa gcgtaagaac
     8641 ttgctgtata gatcgtccca aaatctgcta caccttttgc agaagctttc tccgccttca
     8701 catgagctaa tccatgagca gcaactggtg ccatgatgat tggtgcagtc agcatttctt
     8761 cgtcaaaatg tgtagttgta tcgggtaatt ctacatctct taatacatga ggaataatca
     8821 aacgatgatt gaatgcccgt tcattttctt gataagtaaa taagtcgcca gcaccactgc
     8881 tgatgtaacc ataaccacca gctggaatca cttgtgaagc cgcgatttct aaatcttcca
     8941 tattgataaa atctactatt ccttcatttg tacctgcttg atatgttttt tccatctcaa
     9001 tgccttctct ctataaaatt tgaatgatga acaatatgat agcgtttaca ctttttatat
     9061 tactcttcga cattgataca ggcaaataat ctgtttacat ttcagccatt ccagatgtca
     9121 ctactctgga agaaatgatt gtaataaaaa tagataaaaa accgatggga ctttgacaaa
     9181 agtagtgtca aagtcccttt ttttgaataa acggtgttca aaagccattt tctcggtatt
     9241 aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     9301 aactaagcga ctttttcaca acctctcgat aaaacggtgt tcaatcagct gaccctcggt
     9361 attagctcaa aaaatggcaa aatatgagga actattttgc catttgtctc tctaatactc
     9421 aggtcagaac gttgacttca cagtctcttc cttaaacggt gttcaaaaac ttgcatctgc
     9481 ggtaataagc ccaaccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
     9541 acttgatgca gggcaggttt ttcacgacct ctgattcacg gtgtttcttt ttcatcaaca
     9601 tttttacgaa atactgtgga agactattga aataagtgtg gattgtttct ttgtgtttca
     9661 atcgtttctt gccattcttc atttaatttt ggattaccgt tttcttcctt gaactcataa
     9721 ggtcctaatt caaaaatccc agacagtatt tgattcacga tttcatatag ttcagtgtga
     9781 tcagacacat gattcttgaa gatcatctca ttatcaccac gtttgaacct cacattcggg
     9841 ctctcatgat gaacctctat gcatttttct ccttttttta actgacggac ttctgaaaac
     9901 atctcataaa tatcttctcc agtaaaacct tcagcaagcg ccagactgtc cacaagaccg
     9961 cctactgtca gttcagagat tattgcttga ttggaatcta attttaatgt atagcttgtc
    10021 gcttctacta cgccacatgc attgcaaccg ccttctgata gttcgctttt tctaatcaat
    10081 tgaacttctt tcattttctc ttcctcctct taattttcta cttatgtatt tttatcacct
    10141 gacataggtg actcctatca acgctatcat gtgctttttt cctcagaaat cctatttatc
    10201 cattccatct atcccttatt cttctacttt tctttctcta gcatataaga gatacgcata
    10261 gtattcaagc aaggattgtc gatataaaaa gaacaaatac tgctattttg atttcttttg
    10321 gctatattac atataaataa aatttatcat acctgctctt tttgactata tcatcatctg
    10381 acttctatac ttcgtttgta ttaaaactta ggaggaatca ttcatgctta cagggttaaa
    10441 cttattaaat aatccttttc taaataaagg aacagctttt acaaaagaag aacgggcaaa
    10501 atatggaatc actggtatgc ttccaagtac tgtccaaaca ctggaacaac aatctgttca
    10561 agcttatggt caatacttaa gcaaacaaac agatttggaa aaacgtatct tcttaatgaa
    10621 tctattcaat acgaatcgga cattgtttta taaattgatg ggcgaacatt tagttgaatt
    10681 catgcctgtc gtttatgatc ctgtcgttgc ggattcaatc gaacaataca acgaaatttt
    10741 cttgaaaccg caagatgctg catttctttc aatcgacgaa ccagaaaaca tcaaagcaag
    10801 tttgaagaac gcggcagatg gacgagacat tcgtttgatt gttgtgactg atgcagaagg
    10861 aatcttaggt atgggcgact ggggtgtgaa cggtgttgac atcgctatcg gcaaattgat
    10921 ggtctatact gctgctgctg gtattaatcc agcacaagtt ttgcctgtat cgatcgacgc
    10981 tggtactaat aatgaagaat tattgaataa tcccttatac ttaggcaatc gtcatgctcg
    11041 tgtagaaggc gaaacttact atgaattcat cgatcaattc gttcaatcag ctaccgaatt
    11101 attcccagaa ttgttattgc attgggaaga ttttggacga ggaaatgctg cagcgatctt
    11161 agaaaaatac gaagatcaaa tcactacttt caacgacgat atccaaggta caggaattgt
    11221 tgtcctagca ggtgtattag gcggattgaa tatatccggt gaacaattga aagatcaaat
    11281 aattttgaca tttggtgctg gtactgctgg tgtgggtatc gcaaacatcc ttttagatga
    11341 gatgatccgt caaggtgtac ctgaagaaga agcacgccaa catttctatc aagtagataa
    11401 acaaggttta ttgtttgaag atacagaagg attgactcct ggtcaaatcc cattcgctcg
    11461 caaacgctca gatttcacta atgctgatga attgacaaac ttagaagcag ttgtaaaagc
    11521 gatccatccg acaatcatga ttggtacttc tacccaacct ggagcattca ctgaagaaat
    11581 cgtgaaagaa atggctgctc atacaccacg tccgatcatc atgcctttgt ctaatccaac
    11641 aaaattggca gaagcaaaag caaaagattt aatagaatgg acggacggaa aagcattagt
    11701 tggaacaggt atccctgctg ctgatgttga atataatggt gtaacttacc aaatcggtca
    11761 agcaaataat gctttgatgt acccaggttt aggacttggt ttgattgctt caacagctac
    11821 acgagtaaat gctgaaatca tttctcaagc tagtcgagca ttaggcggaa tcgttgatgt
    11881 aacaaaacca ggagcagcta tcttgcctcc agtagcaaaa atcactgaat tttctcaaac
    11941 gattgctgaa actgtcgcaa aatcagttgt cgcacaaaac ttgaatcgtg aagaaatcac
    12001 ggatatcaaa gaagcagttg aatctgctaa atgggttcct gaatataaat ctttagaaga
    12061 ttaagatcgt tatagtaaaa gaacatacag aaactttgta tgacgtctaa aaagaaaaaa
    12121 gcgactgcgg caaaacagcg gccagccaac tcctgctctt ttgtcgcttt tctttggctt
    12181 ttttcatatg atacagaata agaaaggatc tgtaaaaatg gcacaaaaaa caacaactgc
    12241 aactgcagtt gaggaaaaga aaaaagaagg tttttggtcc acaaaaatca ccggcgtaag
    12301 tttgccactt tatttgatca tggtccttgt cctgatcatt gcaatggctt tagggaaatt
    12361 accgacgaat atgattggac caatcgcagt tttggttatt ttcggtaatt tatttcattt
    12421 tatcgggaca aaaatcccta tcgtcaaaag ttatttgggt ggtggttccg tttttgcgat
    12481 cttcgcttct gctgcgattg ctacatttgg gatcttacct gaatatgtag tgaaatccac
    12541 agaaaacttt gtcagcaata tgggcttttg ggacttttat attgctgcct tgattactgg
    12601 tagcatcctt ggtatgaacc gtaatctatt gatgaaagct tctgttcgtt ttattcccgt
    12661 tgcacttatt tcaatggtcg tctccttttt tgccgtagga ctcgtgggca tgcttatcgg
    12721 aaatggtttt gcaaattccg ttttatacat ctcattacct gcgatggctg gtggtgttgg
    12781 agccggagcc gttcctttat caacgattta tgcaaatgtt ttaggtacgg atgcttcttc
    12841 tatcatttcc cgcctgattc ctgcgacagc aatgacaaat gtcttagcta tcatcggtgc
    12901 agcattagtt gctcgtgctg gacaatcaat gccaaaattc aacggcaatg gtaaattaat
    12961 ggaaaaaggc gatcttggcg atggaaaacc tcgaactgtg aaaccttcca ttccacagtt
    13021 aggtgttggt ttgatggtat cattgacttt cttcatctta ggacaaattg gtaactattt
    13081 tgttcctaaa gtccatgctt acgcatttat gattattatc gttgtcatct gtaaaatcgc
    13141 caacctatta ccagagtatt atgaagatgc agctatcatg ttcaataact tgattgtcaa
    13201 aaacttgaca gcagctgttc ttgccggtat cggtattgcg ttgctgaatc tgaatgtatt
    13261 agcttctgct ttgacatggc aatttgttgt cttatgtctg acaagtgtta tcgcgatttc
    13321 agttgtttca ggatttgtcg gtcgtttgtt tggtttatat ccaattgaat caactattac
    13381 tgctggctta tgcaacaact cgatgggcgg aacaggaaat gtagccgtct tatctgcagc
    13441 taatcggatg gaacttattg cttttgctca aatgggcaat cgacttggtg gtgccatcgt
    13501 tttgatcatc tctggtttct tgatgcaatt actttcttag tcaaaaaaac aaaaatacct
    13561 gatcatcaag tatcggcttg gtgatcgggt atttttttga taaagattta caggattttc
    13621 aaaaatcttt tttgcatttt atcggaatga taaccgatgt gttcataaaa ttggtgtgct
    13681 tctactctgt attctgcaga attaagcctt attgtcgtta ttcctcgttt tttcgcttct
    13741 tcttctacgc cagtcatcaa agcctttccg atgcctcttt tttcagcata tttagacaca
    13801 gcaagtgcca taatattgaa cattggatcg ctaaaaatcg attcatagga ctccgcttgt
    13861 acatagccag ctgctttttt agaagtagga tcttcataaa ccaagaaaaa atttttctct
    13921 ggctccttca gcagtttctt catttgtctc accgtaacgg ttaatggtat atcataccct
    13981 aattgatccg cattgatttc acgtagtgct tctgcatctt ctaaacgcat ttcccgtaac
    14041 atccaaatca ccagccaatt tctgattttt taaaatgaat ttttttatca tagtatactc
    14101 tttttttcat ttagaacaat atgtttcagt gattttgact tgcgttagtc tagattctcc
    14161 taatgtttgt aagatgacaa aggcattgta acaattgacc aaaagaacca cgaggacaat
    14221 tacttgaaaa ataaaggtca ctttttttgg agacaaaacc ctgctaaatc ttgctcccca
    14281 caaacctcca ataacagcag cggggataac ataaaacaac atagataaat caaagcgcat
    14341 aaagctactt gttaaagcaa tcgttaccaa ttttgccagt tgagaaaaga aaatcgtaga
    14401 tagagaatat agtgttgctt cttttattgg caatgcaaac atcagcatca acaaagaaac
    14461 attgactggt ccaccaccaa tacctaaaaa actagctaaa aagcccagaa ccccccccac
    14521 atataaggta ccaagtccat gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga
    14581 agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg gatcagctgc acatgcttct
    14641 cgttttcaaa taactgtaaa aaaacttcga atacaatgtt gccgactact cctccaacaa
    14701 cagctccact agatacccaa ccgacaatct gccaattcag tgatttcccg cttgatactt
    14761 gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc
    14821 ctgcaacaga atgggcgccg ataagatcaa gaatcggttt gatcaacacg cctccaccca
    14881 tcccggaaat agcgccaact gtgttcgcca gaacgatcac cacaaaataa atcagaccaa
    14941 tcatcttgta tttcctttct cttgtttata tcttttagcg attgttactc ttttctttta
    15001 ttatacagta aaagaaacaa acgaggcttc gaaagctttt ctacttttta aggaaattta
    15061 gtgctaaacg aaaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa
    15121 acaaaaatag ctcctcataa ttttcgtttg gttgaactta ttaccgcaga tgcaagcttt
    15181 tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca
    15241 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttggc
    15301 aggagaacac cgtttaagga agagactgtg aagtcaacgt tttgacctga ttattagaga
    15361 aacaaatggc aaaatagttc ttcatatttc gccgtttttc gagcgaatgc cgaggggcag
    15421 ctgattgaac atcgtttatt tggaaaaagg gattgtgaca agacgtttgt cacaatccct
    15481 ttttttatat cttattaatt cattcttttg ttctattctt cttcttccgc catgaatgag
    15541 aaacccatat caaagaacta taaaggttat caaaacagcg tttatgacta aatgaattag
    15601 agttatctat cactattttt caagcgattt aaaccaaatg gcatacaata tggcatacaa
    15661 aaacttcaat aagccgtggg ggtttttaaa tttcttcttg ttctttaaaa tcaatgagtg
    15721 gagctacata atttgaatcc actttttcaa acgtcacttt ttgcatcgac gaatgttttc
    15781 tttcccaaga taacgtaaat agatttgact ctggacttat aaagtctact tcaccttcta
    15841 gtattacttt cttatgcaaa tattcctcta ataaatatag acttgctaaa gcatacaaca
    15901 cattttccaa atttgcttcc ttataatttg aagaacgacc atgttttact ctgttatata
    15961 ttttccacca atctggtgta tcttcaggcc acccatctcc aaaaggtttt agtgaagtca
    16021 atgttaaaga attcataacc ctaacaccat tatccaaaca cattaattcg ctagatttaa
    16081 taacagcttt aaaatcagtt atgcttttat tgctactgct atcaaatccg caaagttctt
    16141 tgaaaataat ctcacactcc gaaccaatcg ctagtagtag tttttgatat tcaatagaga
    16201 atgttttgaa attattctca tcgagcatca caaaatctgt ggtattaata aactcatttt
    16261 ctaaatgtag ataatacttc caataaaaat ttgtaaactc tgctttgtcc attaaatcac
    16321 ctcttttgaa ttataacaaa aaaaacacgt ccacaacaga cgtgttcttt gatttctaca
    16381 caatcagctt gccaaaacca tccaatcagt cctagcatgc ctgtattaaa attgaacgtt
    16441 catcatctat catctttctt agtccagcat gtttgcaacc acacctacaa ttttcagtcc
    16501 aacaatagat ttcgactagc taagtcttgc aaaattcagg cagctaaccc ttcatttact
    16561 accttaatta tactaaatac ccacaacgtt tacaagtgta atcattctaa gaaatctgtt
    16621 tagctatgat cgttgtataa tctttaaacg ccgttccttc attgattgaa ataattttat
    16681 aactcttgtt tttccaagct actttcatat cgtttgttag ttcataatct tgatcgtatc
    16741 gaataataaa cgtcaacgtt ccttcaagaa tggtacctac actagcaagc acgtctttta
    16801 gcatttgtga acgcacatta gcccaacatt caaatacagt ttcttcacta tccaccatct
    16861 gcccatcttc atcttttacc cgttttgtct ttttaaactt gactctctca gtcaattcat
    16921 ttacattctt aatgattggc ataataagca cctctcattt gttgtactaa tgcttgtacc
    16981 gttactggta tccgttcgct gctggcttct tgacggttca aataccaatg ttgagtaagt
    17041 aatgacaccg ccaaatcaaa ttgctgataa ttatccatat ctttatccgt tgcgctacta
    17101 tcaatagcac tttttatata ttcgcctgct gtatcaatca attgttgaat aaagtcatca
    17161 tcaatagtgt gatcgatccg catacttttt tttatgctat ccaaaattac cattttatct
    17221 ctcctaatga aaaaaaggca gtgggataaa ccgccgccca atatttttct atacttttgg
    17281 tgttacaact ggcgtaaact caatatacac tgaagcattt tgatcaattt tcttataatc
    17341 gttgcgaacg atcactgcaa gtccttgtga gtagtaatcg aatttttccc attgagtcgt
    17401 tacttgattt ctacgagcaa caaagataga ttgtgcaata tcccccataa tcatagggaa
    17461 tgttccagct ttaggattgg caaacaatgt atctgcaatc aatactactg gcataccgaa
    17521 taatgattta ccactaggtg ctgtcacatc gggttgtaaa atgtaacgtc catctgaatc
    17581 tttcaatgta tctaggtggt aatatccgct ttggtttaga attaccattt tatttaatgc
    17641 tgggtctaat gccacattgt atagttgttt caaatcgtcc aacgtagcag ccgttttctt
    17701 agtgaatgtt tttaataaat ccatgatatg cttattgtca gtgttttcta ccaatttcgc
    17761 taactgatct ttgacctctt gtacaatatt cactgctgaa tcttccacaa cctcatttga
    17821 taaggcaatc ttaccagcac gagtttctac tttataatca actgaagtaa acatttcagc
    17881 gtcaatatca ccaatttcag caagttcagc ttttgttgct aacactgctt gttggttagt
    17941 tgctactgga tatttacctt gtccatttga tactgttttt actgttgcat attgagctaa
    18001 gttataattt gaacgtttca aatcaaagac ttcaccgatt acttcttcgg gaacaactgc
    18061 cgctgcattt acagtagtga ctccatcacg cacttcgcct tgtgaacgga tatattcttc
    18121 atacccacgc aattctgttt tttcgttatc gattaaagtt ttttccatgt tattactctc
    18181 cttttgtgct ttagaagtgg attctaagct attgtttgtt tgttttgctt ggttgttact
    18241 cataaacgat tcgtaagaac gcttatttac ttggacattt gatgaatcat aagcgggaac
    18301 ggtcacgaca ctaatttcat taagtgcttt gattttatct attgatcgtt caattgtgcc
    18361 atcttctttt ttgtcgaatg aatctaatcc aagcacgaac ccaaaggaca tagaatcaac
    18421 aacccctttt gagatattct catacacatc atttgaatag gtcgtgtcgg ttagctctgc
    18481 ttcaaaatgt aatcctgtat catcgacatt caatttcaac gtacctgctt tgacactagc
    18541 tagtggcttt gaataatcgt gtccatataa taagaacaca tttgataaat ccacctcttt
    18601 taatgcttcg ggtgtaatca cttctacaaa gccgcctaaa tcttttgacg gttgcccgaa
    18661 tttcaacgca tagcctgaaa tagttttgcc gtctgttggt tgtttttcat tttctttctc
    18721 tactttggga gaatcggctg ttagctcagc ttcttctgtc agccgttttt ccttttcttc
    18781 ttcattattc aactggtgta actccttcct gttcttttaa ttgatatttc tccaacgtat
    18841 ctaaatacgt aaagttcaaa cttgctagta aacgatcgcc accatccatg gggggtagcc
    18901 ccattttcga ccgcccctca ttaatcgtga gaagtgaccc tttgacctga tccaatacat
    18961 tttttacttt tgtttcagga tcagtctcta gtaactgatc ggaattaaag cggatattgg
    19021 tttcaagttt cgtatctaat tcactcacaa acacattaaa gtaatgaatc agcgtacttt
    19081 ggatatactg gagattactt tgaacggtgc tagaatgttc attttcaacg cctaaacgct
    19141 caatcggtac accaaacgcc ttggcaattt gttttgtgtt ccaatcattg gaattgataa
    19201 gcttcaaaac atcggtgttt atttctaatg ttttgtagtc cattgtttca tcaagaatga
    19261 tcgttctaag agcattatct ccactacttg aaccgttggc ttcttcaaac ttttcacgaa
    19321 ttgcgttctt tgctgatccg tctaaatctg acttatgaac cttcaaaata cctgacccac
    19381 tgacaccacg agtaaagaaa ttgtgcaatg tgcggttgcc agcttgttga attttcagtt
    19441 catcacgtaa cgcataaagt ggtggtaatc ctgttaaacc atcttgggtg aaatatttga
    19501 agtgcaaaat atcgctagac ttcacacgtc tttttttatc accaatctga taagacaacg
    19561 taccattatc taattgagta accgttacac ttgagttagg taataagtga atttcttcta
    19621 ccttttcacc gttgcgttta atctctgcaa agctattacc gtttagcaac atattgaccg
    19681 ccaaagcaaa ctttaaatgc caaccatcca tttctgaatt aggtttctcg tttagtaact
    19741 tcaccagttc attatcagct tgtggcatat tgtttttgac caattgaatc ggacttgaag
    19801 caatatcact agcaataatt cgaacggctg caaacacatc actatttctt aatgcacgaa
    19861 cgcttgtaaa gctggtggtg taatcatccg attggattga taccacatgt tgtaggaaag
    19921 gatctcccgt tgtattttgt ctcgctttaa aaaatgccat cttttaacct cccttctgtt
    19981 ctgtatttag catgaccgct accccaataa gcactatgcc cagcgctaac aagccatagt
    20041 agacatttgt tagtaaggtg atagcaacca acacacacac caatccaaga agtaaaagaa
    20101 cggtatgaat gtaattagaa agaaaattct tcacttgtat aaaattcatt gtctgctttc
    20161 gctccttccg ttgtggtgaa gtaatccatt gcaaacacat aggcattgat taatgcagct
    20221 attggatcaa ttttgttact gttctttgat ttattaatct gaatgccgtt gttatccgtt
    20281 tttaaaatag cgttgttcac tgcatgggtg agaatcgtat ttttattgtg aataacgttg
    20341 ccttcataaa cttgttctct aaaagttcgg gtcggaacgt ttagagtaat cgttccttgt
    20401 cttacttcta acattgggta gttggctttt tcagctttac tgataagcga attagcgtta
    20461 tacgggtcat aacaaatccc catacattcc aaatcatttt cttgaatcaa atcttgaata
    20521 aactgaaata cttggtcata gtccacgatt ccactttcta attgagtaat ggaacactca
    20581 ccagcttttt ctagttctcg ataaggcaaa ccgtcgcgct tttctttgtc ttgaagccca
    20641 tatttggtgg ctacaaagct atgtgaatca caataaagtt cgccgttatc tagcggtacg
    20701 atccatgaaa cgctagttaa atcatctgtt ttagataaat ccactccgat ataaacgggc
    20761 ttgcctgtaa tatcttgtgg ttctacttcg gttgcctgcc agtcttcagc aatcatatag
    20821 ctgtcctcac tcgcttgtct ccataaattg aagtttttca ccaatacaga attaaggtta
    20881 ttttgtttca aagcaaggtt cacatcatct tgaatggttg gaatcattac ttttttgatt
    20941 tcttcacttt caaaaatcgg attggctttg atccatttct cttgatcgtg aatttcttcg
    21001 ggatcgtcca attcccatat cgctataaag tacctgtcgg cttgttcttc gccttttagc
    21061 acacgatcta acaataagta ttcctcgtac ataggaacgt ttaaatcaag cccagcggta
    21121 ctaataacac acaataagcc gttcttttgt tgggtcattc ctgacttaat gacgttgtac
    21181 gttttacgag tttttgcttc gtgccactcg tcaagaattg cggttgttgg tgcaaaccca
    21241 tctagcgtgc tggtttcact agccagtgcc atagcgaacg aattagaggg caagtgctga
    21301 atcttagagt tcataatttt cagttgtggt cttaaaaact tactagactt gaccacgttt
    21361 ctaagtgaat tagacagcat atcatagccc aatttagctt gtttcaaagc gttacttaca
    21421 aacaatactt gccttgcttc tgctggttct tgttccataa tcaacgcatt ggcagccatg
    21481 ccacttgcga tatacgtttt cccattctta cggctcatac tggtaaacgc tcgattgaat
    21541 cgcctgtaat tcccttcttt agtacgccag ccataaagac tgcctagaat gaatttttga
    21601 aatcctaaca tcttgattgc tttaccgtcc gtagatggta gcaactcaat gaatttaatc
    21661 gctttggtgg cttgttcttc atcgaacaca taagggaagc tatctgactt agaacgctcc
    21721 aaatcgctta tatgacgctc acacgcttgg ataattttct tgggtgcaat cagcttacct
    21781 gataggactt tctcaatgta atcattcatg aattcatcaa ctctttgaac gggtcttcgg
    21841 gttcatcatt tttaaccttg ttcaaagcta gttttgatcg tgattctaag gtcataccta
    21901 gtgaggtggc ttgttttttc aaatcactca tagcttgtga ttgaacccga accgctggat
    21961 tggctttctt tactccgttt tcgcatgtga taaacgtacc tgattttctt atctcggctt
    22021 cggctgtttt cattcgagca aaggctaaac aataggctac caatgaacca taatctgctt
    22081 cactcaatgg ataatctttt tttaatgttg gtacgatacg attccattca cttctagcgc
    22141 tcgctgccat ccagtcaggg ggcgtgatat ttattaatgg ttgctgcttg aacaattctt
    22201 ctctagcgtc cacacggatt gctttttctt cacttgatat attgcctttt gtatcgtgta
    22261 atattttagg gtgtcccatg ttgtcctcct ttcagacaaa agaaaaagga acgtaagcaa
    22321 ttaagctcac attccttcgt ttttcgatca gaatttttct ttatctaatt tatctatata
    22381 aatgattttt ccgttctgca tttgaatggt cacttgacca aaatgaggta actcaatggt
    22441 gcgctgtttc cctgttttat cagtaataac aattgtattt ttcaataaat ctcctacttt
    22501 cattatatac tttttcactt gtgattacaa gtgtaatcgt tatttgtaat tacccttatg
    22561 ccttttcaca acggttattt gtaccaaaat gagtaggcat cgattgtcag taagtttcac
    22621 actatgcccc cttaactcaa agggtactct tttctcgtct ttgtctttca ttggctgtct
    22681 tggaattatg gtgtgcatgg cataaagaac gaaaattagt ttcatctaac cgtctattcc
    22741 aatcatcttt tagttcaatt ttgtgatcta caacatcagc tttccttatc aagccttctt
    22801 ctaggcaatc ctcacatact ggatgattta accgatacaa gtatgaagcc tttcgccatg
    22861 ttctactttg atagaattga aaatatttac cttcgctttg ctttctttct tcacgcttaa
    22921 ttgccgtttg ctttgcttta tgcttcccac aatacttctg cctataatca atcaatgttt
    22981 tacatccagc atgattacat tgttttttaa ctgccataga ttaccaacca aagcgatctc
    23041 gttcgtcaga atcatcttgt tcccattttg atttgtattc aatataaggc gaaactatct
    23101 tcaagcacgt tccgtcttta aaattaactt tagtgatttt atcttcctca aaactaataa
    23161 agtctacatc ttcttgagtg tttgaatact caatgtgttt ctcaccatcg aagtatctaa
    23221 tactttccat atgcataatt attctacctc caataaatta gttatcaatt catcatctgt
    23281 tagtagcaaa gccaagaact tatcatggat gtttaaaaac aaattatcct cattcgatgt
    23341 aagctctcgt tgtctcgctc gatcatatag gttaagcaga caattaaatt catccattag
    23401 ctcacctgta tcatcatagt tgccactatc taccaatgat cccactacta gcgattgaca
    23461 ccacgctact gactcctgca gccaatcagg ttcttcttcc attatcttta atacttctgg
    23521 atcaaatgtg gaataatctt ctgttaccaa tcccacacta aatgccagcg tgttcattag
    23581 aaaccgatcc atttcatctg tatattgaaa gttcggtgct ttgtttgttg aaataatatt
    23641 gagcatttac tttcctcctt gattcgtaac acgtggtaac acctacgtaa caggtacccg
    23701 ttaccactca atcccaacgg ggacaagggt tctagcgttt tggtaacagg taacgggtaa
    23761 aatccagtaa cattattccc cagcatatta gctaactatt tcaaaagttt taaggaaata
    23821 aggtgttacc ccgttaccaa attgctgtat cccttggggc agtaagggta aactagtaac
    23881 gggctaggtg ttactgaccc gttactaccc gttacttttt gtactttcca gctcgatttt
    23941 tattttcata ggcatttgac ttattgtaaa atcctcgtcg tttatttctt tttggcagtt
    24001 gcttccgttc ccaaccagga atactgctaa taatcaaggc aatcttttta gtttctgcat
    24061 ctgatcgacc attcaataaa tcctttgctt gtttatcaaa aacagcttcc aaaatatcag
    24121 cggtgagtac gctatcaact aaataaaact cttgttgcat agtagctcca ttatagaaat
    24181 gctgtaaatt tttctcatta catttactgt caggatagtt ctgataataa gaacgtctcg
    24241 cccacgctgg agcttcttcc catttatacg ggatttccat ttccaaatat ttagtgattg
    24301 cttctttaat tggattctcc atcttggcat cctcttgata gtcttttgca atttctaaga
    24361 cttctttatc ttcatcggga ctaaaataga ttcgttgtcc tttatcaaaa agcacctttg
    24421 cttcagctaa cacttgaaga aaatattcat cctttgtttt aaacggattc atctttggct
    24481 tattcttaca aggcaaagga taaaaacggc ggtttcctgt atcatctttc aaatactgcc
    24541 catcattcga cgttccaata aatacacatt gccttgccca agctatgaca ttccgaccat
    24601 aaggaggtct aattttatcg atcttggcac tgatgaagtt ttttatctgt tcaattttag
    24661 ttttgttcat tgaagataat tctccaagtt caataattac attgccttga agctgcatat
    24721 aatcatcttt cttttcacct agcgaatcta atgaatcggt gaaaaaatca tccgtgtaaa
    24781 gtaatgctgg aacggtactc ttaccaatcc cttgcggtcc atccaaaact ggaacgatct
    24841 caaactttat tcctggttta tagatacgtg cgactgatcc gactatccaa cgttttgtta
    24901 cttctcgtgt gtaaaggttg tcgtctaccc ctaaaaggtc aataaacagt gtttccagtc
    24961 taggctgttg atcccattta actttttcga tacgttcttt tactgggttg aaaacattct
    25021 ttctagccac atttactact gctttgtgta ttttgttgtc atcaaaaact acattgtagt
    25081 tttcttcaat atatgccgtt aaggcatcct caaaactgtc ttcccattct ccagcggtac
    25141 gattgatcca aggagaatct tttaataggt gaatgtatcc gctaaattcg ttataagcaa
    25201 ttgttccagc taaattgcta tcattggtta aaatcaattc tgcattcaca acactgttac
    25261 ttttaatctg atcgtcttta ttgcatctta atcgtgacat ccaggttttc aattcaatga
    25321 tattgtcttc acgctcgctg gcttcgggaa agtctaaaat actatctaat ttatgcttat
    25381 gagccatgag ctttccccct caaatctttt ttaatcatcg atcgaataat ttgattcact
    25441 tctttatctt ttagcggtgg ttcattgtac gcatttgctg tataaatcaa ttctgctaaa
    25501 gtttgattat ctacaccgtg ccatagtaac caaccgcata acttagttaa atgatcgttt
    25561 ctactacctt cgccagcgcc ttgaacaatc atatctaatt tttttgctgt ccctgttttt
    25621 gctacgggtc ggtattgagt ctttatttct tgcggttttt ttagcagtag gttcactagc
    25681 caatcgggag ctggtgcaat attatttaga gatacgtttt caataggttc atatgctctc
    25741 ccaacacttc ttgaaataat catagttggc gctattggaa ctcctttagt gataatatca
    25801 atcccgctta aacttttacc tttatcatca cgcccgaaga tatcagcaaa tgcacctgtt
    25861 tcttgttgga taattacacc atcaggaatt ttaaagaaga aatggattcc tttacctggt
    25921 gtgatttccg tataagtaga aggaaatgga tcatacattg aagcaagttt tttataattc
    25981 tcaacgccgt ttcgtgtttc agaatgttga tctaggtcaa ctaccaccat ttgatgatct
    26041 tcaagcatta acccaatatt tctagttgca tcttccgtcc accattcatt aatttgatta
    26101 gaatcgttag ttgcatcatt ccaaccacta gagcctttta ttggaatttt agttccagga
    26161 gataagggat aaattgacca tcccgacttt tgtaattccg ttgctgcttg ataattattt
    26221 gccaataaac ctctcctcct ttttcaaaat actggtataa atcattcaga cacctccatg
    26281 agcttcaact gctcaagttc gtatttcaac ccatcccgaa caaattcagc accttttaat
    26341 tgattctgat actttaccca tgtattaaat gttttcaccc ttgcttcttg catatcagta
    26401 atgtttaacc atgcggttaa aatctgattt tttagctcgt tgatttctcg acaaattatt
    26461 ttgtgttctg catgtttttt gagtacatac aaaactgtta gtttttcatt gaatgaattt
    26521 ccaaagtagg ttttcccgat attattttct agttcagcga tcccttgatt aatttcctct
    26581 aattcaaggc tatcgagttg gtttaatagt tttgttttgt acatctactt atgccctcca
    26641 gttcttaatt gatagtcgta ctcgtcataa gcagtgaaga gatataacgc taatcctgca
    26701 aagatagcta tttttgcaat taatggtaat aagccgaaaa ttccgccgat taaaatgctt
    26761 cctataatta agtttcgttt cataatggtt ccctccgatt ttttaatatg gtataatcgg
    26821 aacaaagaat atcgggtttt atcccaatta agttgccttt atcctgtccg ccaagactgt
    26881 tatggataag gctttttctt tgttctcgat catgttaatg ctcctaagtt gtctgcaatt
    26941 cagcagaacg attataaatt ttctcactca ctgaaaactg caacccatat ttttctttaa
    27001 cagtcattaa ttctactgtc tcatctaaaa taggttcacg atcaaccaac atttcaggag
    27061 tcatatcttt cttttttacc attttggaat gaccataaat agttgataca gctttattgg
    27121 ctatcacatt cgctttaatg tagtctactg gttttggttc agtaatagcg tggcttaatt
    27181 tagtcattgc ttctttttgg tgttctttgt ctagcatgcg gaatacttga aagccttcaa
    27241 gtcctgtgga ctggcgtagt tctttgatga tgtcgaaaat ccagtcttga aatgcctcgg
    27301 cttctttttt acggcttttg aatgccatgc ggtaaatatt ttttttcgtt gatgatgttt
    27361 acatcttgtg ttctgcccaa tgaatcgatg accttactag tagtaacccc atctttaggc
    27421 aaagagtgaa tggctctagt aacctgttta agacctaacg cttccgcaac atctttcgcc
    27481 actgcccacc attcatcatt aatatcgaca aatcttataa tgtgtccgtt ccaaatttca
    27541 gttttcattc aacgtcctcc agttcaagcc ctagcaagct cgctaacaac tcaacttgct
    27601 gatacaaaat ttcttgatac tcttcaatag aaagtcgttc tttaaactct gcttgtacta
    27661 aatcgttcaa gccacgcaat actccgctta ccgttggttc ttgtggtagc aatttttgat
    27721 agtccatttg ttcgtacaaa cattcaaacg tttcttctag cgctttgcct tccaagtttg
    27781 ctaattctgc cgctgtgtct tctgtaagaa ttgttgataa tactttttcg attgctttat
    27841 tttgtgtcga ttccattatt atttcctcct attaatccct ttgcccccac ggcttgcgtt
    27901 tatttttggt attcttccat aaagcgatca cattcatctt gtgaaattct tacgactcca
    27961 tcaactataa tcaactttaa accgttagga acatatttct gcatcatagt attgtatgaa
    28021 acgttcatat acttagcagc ttgtgccttg ttcatatatt ttggaaactg gtactttttt
    28081 tctaataatg aaagtaacgt tccttgagtt tgctctactt gttgaacaac tcgatcgtca
    28141 ataattttaa aagctggatc atcaagcgtg agtttgtcaa tttttaattc catatttatc
    28201 ccccctcgtt agcatacatt tctatacaaa gattttaaaa aaatgcaaga ggctcttgaa
    28261 tacatttcta tacctcttat aaactcaata taccatcata ccttttcatt tgcaagaatt
    28321 ttaacatact tttttgaact tttatgtttc acacatattt aaatcgtgat atagtagaga
    28381 aaatatatga atggtggtga aaatgtggct agaaaaaaat accccgaagc agtggagaca
    28441 ggacaaagaa ttagactggc tagaattgaa aagggactct caatggaaca acttggaggt
    28501 aaattatccc caccggccag taaaggtgcc gttagtaatt gggaaaatgg ctataatctt
    28561 cccaataaca ccaggcttaa acaactttca aaggtattag atgtatccac tacctatcta
    28621 ttaaacggat cttatacatt aagagatatg catatgatgc ccgaaattaa gcgaaatgtg
    28681 cttgaaaagc aattgaaaaa aactattgaa aaaaataact ctataattga ttctaatctt
    28741 tctaatgcta tcaataacat cgaagaagat aacctgactg tctcacagaa gatttattta
    28801 acaaacgcaa taaaatatct taaaacagga tctcgaattg aatttcctga cgggagtacc
    28861 ccaattgctt atctcgcagc tcttctaaat acgcttttac gatatgactc attaattaat
    28921 gattcagaaa aaaatacaga tgagaaaaaa gagtatcaag aattttatga aaatgatctt
    28981 ctaaaagagt tcacagctct tgttactgag ctaaataaac actattcaaa ataataataa
    29041 actgcctcct ccaaccgccc ccacggcttg ctacactatg gagggaaaac tattatggca
    29101 acattcaagc aatatgaaac taaaaaaggt aaattttggc tctatgaagc ctatcttgga
    29161 atgaacaaaa tgactggcaa acctgataag gttcgtagac gaggttacaa aacaaaaaaa
    29221 gaagcccagc ttgccttgtc acgcttgcaa gtggactacg ataaaaacgg tattaaaaaa
    29281 gcgaacaacg aaacgtttaa agaagtttat gacttgtggc tagaaacata taaaaccact
    29341 gtccgagaag ccaccttcat gaaaactgaa atcaagttta gaaagtggat tcttccaaaa
    29401 tatggaaatc ttcgtgtgaa tgaagtcacg gtaaaaaaag cccaacaaat tgtaaatagc
    29461 tgggctgaaa ctactgacca atacaaggta cttcattcaa cttctaagcg tatttttagc
    29521 tacgctatca atcttgggat tattgagact aatccactag acaacgtatt aatgcctaaa
    29581 agaaccacca ctaagcataa agaaaaggtc aaagtttaca gtaaagaaca actgatcacc
    29641 ttgtttgatt acctaaacag caaacctgaa acttatcgaa acgactttga taaaacgtta
    29701 ctacgctttc tcttttatag cggggttagg atcagtgaag cccttgcatt gaattggtca
    29761 gacattgatt ttcaagaaaa tacggtcacc atcaataaaa cactaagcca atccaaaaat
    29821 ggctataaaa tcagtgatcc taaaactgat tgtagtgctg gggttcttcc actagatgaa
    29881 agaacaattt ctatattaaa aaaatggcag attaatcagc ggaaatatat gctgacactt
    29941 ggtgtaactg atccgactat gattttttgt ggtatctata aagaactaat cacacaccac
    30001 gctatttatg caagattgat tactatttgt gaaaaagcta tggttccatt tctcggtgtg
    30061 catgtagcaa gacacactca tgcttcaatg ctcttggacg ctggagctac tatggtagaa
    30121 gttcaaacta gattacggca tacaaaaatt agtcagacga tcgattgtta cggtcacttg
    30181 gcaaaagaaa cgaaagaaaa gactgttgaa aaattagtac aacacttaaa tattgactag
    30241 atggcataca atatggcata cagtaaaaaa taaaaaaagg agaaacccta tcatatcaag
    30301 gtttctccta gctttatagt ctattcttct tcttccgcca tgaatgtatt gaatacttct
    30361 tcaatcatgt cccattctgc gtctgtttcg atttgttcta actcgccttc tgttccatct
    30421 tcgttttcga tgtatgcata agcttgtaat tctacatctt catcttctga aacaccagca
    30481 ggatataaaa gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt tagtaggatt
    30541 tcatataatg tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg tccttcatgg
    30601 tcgtgggaat ggttatgttc tgtcataaga ttacctcttt tcttttatta ttaaaataat
    30661 tgcttacctg agccgtctaa ataattttgt aagatcatca cagctgctaa tttatcgatg
    30721 actttttttc gtttggctct tgaagtattt gcctgctcta ctagcatacg ttctgcctga
    30781 acagtggtta atcgttcatc ttgataatca actggcaagg agaatagttc tttgatcttt
    30841 tctccgtatg acatggaagc ttctgccctt ggtccgatgg aattgttcat atttttaggt
    30901 aatccaacta caaaccggtc tacttcatat tgtgctacta attccttcaa ccgatcaaag
    30961 ccaaactctt cttctgcttc attgatccga atgatctcta ctccttgagc tgtccagcca
    31021 aaggggtcac tgatagcaac acctactgtc ttagaaccga catccaagcc cattactctc
    31081 ataaattcac tccatgattt gagaggtagt ccttgacaat tacttccatg atctcatcgc
    31141 gttcgtgacg gcgaatcaaa tttcgagcat cacgataacg aggaatgtag gccgggtctc
    31201 cagaaagcaa gtagccgaca atctgattga ttggattgta gcctttttct tccaaagccc
    31261 gataaacaat cgctaatgtt tcgctcactt cttttttgcg actatcgtca aaaccaaaac
    31321 gtacggtttc atctgtaaaa cccatgattc tacacctctt tcccgcaagg taattccagc
    31381 ttaccttcta caaccttatt ttactcgaag aaagggaaaa ctacaaacaa atattccaaa
    31441 cttccgaata aattaagaaa cactatttgt atgttttttt ccttttttct gtagctttat
    31501 caaaagtact cttttcataa agtatacgct atatttcttt gaatgtatgt ttttacacaa
    31561 ttttttcata atattcataa aaaattcgaa tagctgctac taattttttg tttttgttgc
    31621 ttgtcctgtc tcataattga tcgtgtagcc atcttgaaca ttgtagataa agacattgaa
    31681 ttcaattttt ttgtcttcaa tgctttctgc ttccaattgc actcccctgg ctaccagttc
    31741 attcccctca aaatgaggag tgacacggta gcgtacatga tgatttgttt ctttgatata
    31801 tgccgcaata tcattttcgt gagcaagcat gtatggagca ttcaacgaac gtgtccctgt
    31861 catcaagttt ttaatgttgt tattttgacc agtcagctgg aaaccaatta aatggctgcg
    31921 attatataac cattgcccgt cacttaattt cttttgtttc caacctgtcg ggtcaatata
    31981 caaaggctct ctcttttcag tagggaacaa atcttttcca agcatagcat tggcttgtcc
    32041 tactctgttc aatttatcaa tatttgaata gctttcccat gttcctttgg aaaggctaag
    32101 atcctctttt gtaaattccg gctgattccc attgatttct atttcttgat gttttccatc
    32161 atattctggc acatccgcta cttgagaagc ttgtggtgtg gaatcaaaaa tagttgtaat
    32221 ctgctgaaag atatctgaat ctttttgtgt ggtggaaaca ccgaatgccc ctgcaatcac
    32281 agcagcgacg atccccacga tcgccttggt tgtttttttg ctcatttctt tactccttta
    32341 ctaacagctc cctaaatctt aacatatgtt cccctataaa taaatactag aaagaaatag
    32401 aaacagcaaa aaataaaacc tgttttaaga tggcttaaaa atacaaaaaa gagacggatt
    32461 cctcgtctct aatcagtcac cactctatct gtaaggtatt tattattatt caaaaatttt
    32521 cttcatactc aagacaatac actgatttca caaccgcttg gtaaacggtg cgcaaaagct
    32581 tgcatctgcg gtaataagtc catccaagtt aaaaacataa gaagttgttt ttgcttggct
    32641 gttcttatta cttgttgcag agcagctttt tcacaaccta ttttaagcgg gtgacgggaa
    32701 tcgaacccgc gacatcagct tgggaagctg ggattttacc actaaactac acccgcaata
    32761 caaaaacagt atacgcctct tttattcctt cctcaagttt ttttattctt tacgcttctg
    32821 gtatgttttg tagtaaaata aaactaatca atcaattatt ggagaagatg ctatgacggg
    32881 aaaagaatta gatctgttgc tggacacttg tttactcgct gggaaaatca tgatggaaag
    32941 caatgcggag atgtatcggg tagaagatac gatgagtcga attgctttag cttctggaaa
    33001 tttccgtttg gtcagctatg tcactcaaac cggtctcttt attggtcttg atcgtacctc
    33061 gaccatccga atggaacaaa ttaccaaccg ttcgattaat ttagaaaaag tagtaaaagt
    33121 aaatgatctt tcacgtaaat atgttgcttc agagctaaca ttagaagaac tttactgcga
    33181 actgcaagca atcgaccgag accgttcttt ctttcctgtc tggttgcaaa tcgttagcgc
    33241 ggcagtgatc agtggaacaa ttatggtgtt atttggcgga aagatgaccg atttacctgt
    33301 gactttagtc attggaggat tgggatacgc agcttatcta tatagtttga aattttttag
    33361 aatcaaattt ttatcggaat ttttatcttc tttattgatt ggcatagccg ctatctacag
    33421 cgtacgtttt ggcttaggaa gcaatcagga tctaatcatt atcggatgtg tgatgccttt
    33481 agttcctgga gttcaaatta cgaacgctat ccgtgatttg ctagctggac attatgtctc
    33541 aggagtttct cgcggaacag aagcgatgat gactgctacg atgatcggtt ttgcgattgc
    33601 ttttattttc cagctttttt attaggaaag gaacacaagt atggggacga tatttattca
    33661 gttttctttt agttttttag cgactgcggc atttgccgtt attacaaatg tacctaggcg
    33721 ttcattgatt tgttgtggtc tttctggtgc ttttggctgg atgatctact ggatctgtgt
    33781 tcaaataggc ggatctgctg cttttggttc attgcttggt tctttaggag tagcattcat
    33841 cagcgatctt ttctctaaac gtttgaaaat gcccgtaacg attttcaata ttcccgggat
    33901 ggttcctcta gtccctggtg gtttagctta tcaagcggtt cgaaatctcg ttactgaatc
    33961 ctatcgagaa gcggctcatt atgcggtcca agcaattatg attgctggag caatcgcttt
    34021 aggtcttgta ttatcagaag tctttaacca taacatccgt aatttcaaag taaagcgtga
    34081 cgcaatcatg atgaaacgaa aaaaacggct ttttaaataa acgaagtgta acaaagaaaa
    34141 ggttgtgaaa gagacgttca gccttgagcg atgacaaaat atctcacaaa cattgcgaca
    34201 agtaaccgca ggagcaataa gaagtaatta tgagtattaa aaaaagtttt gtaaaaatag
    34261 cttctcacat ttttacataa aattgactta atactgagaa actgactttt gaacaccctc
    34321 tatctgtaaa aaaggggctg cgacatgatt tttgtcacgg cccctcttct ttattcaaat
    34381 tttttccaaa acgttcgttg ataataaacg atccatagag cggaaagtat tagaactaaa
    34441 ccgatcacag gaatattcac taaccaaaat ggataaaaca aagccaacat tccgtataag
    34501 acgataagaa taatactgat gatcaatgaa cccatcatag ttgcgactaa acccaaactt
    34561 cctgaacctc tgttaaataa ttggttaata ttggtccagt tcagcagcag caaccgataa
    34621 tctctagcga aataacgtag tgacaataag tagcagccga ataaagaacc aaatagcaag
    34681 ctagggataa aaaacaaagg cagctgaaaa gacagtccgc ttaataatgc gatcccacca
    34741 gttaaaaaac tctgcaatat ccatccgaat ctgaactttt cttttaaata ttggttcata
    34801 gagattggca aagaacggat aaacagaaaa ttttcttgat ccaaagaaat aagattgctg
    34861 ataaaagaag tctgattaac tgttaagaaa gccaatgcga tccctaccag gaaaacaaca
    34921 ccgattaact tattatctaa atcagttaat tttatttcgc ctcctagggc aaaagcaaga
    34981 ataaaaatga ttggagttaa caaggaagaa gaaagaactt gcataatcaa attaggttct
    35041 tttactaatt gagcattgta actaaataaa agctgatgca cattttggtt ctttttatgg
    35101 gttcttttta ttttgaactc actgtttgaa tcagtcagtt gctctgtaag ctttggtact
    35161 atcagctgtt ttacagcaag gaaaaatccc agagccgtga tagctagtat tccccaaccc
    35221 aataaacctt gttgagaaag tggctgactg gcagcatcat agaatggcaa taatatcgaa
    35281 atcggatgcc gatctgttaa ctcattttca atagtccccg tttgatgatt catccacatg
    35341 attccagcaa atgcgatcag catcgagctg ccaagcaaga tactagaaac tgtttttcta
    35401 tgttttttaa acactgctgt tcgagccaaa ccaaaaacaa tcaaactagc cagacaaaaa
    35461 agaataatca aaaatagaga aaacaaaagg attgagataa tgatagtaag caaaatccct
    35521 agatcagctc gccatccggt taaaataaac atgactagaa gcggaaaaac aaaaggaatg
    35581 atggtcaaag caactaccaa aatctttgct gaaaaaacct gttcttgtcc aaaaggcaga
    35641 ggcaaaaata cttgcaggtc atttccttca aaaaagatat tataaatgac tgatatactt
    35701 tgagttaaac ctaacatacc aaaaagtgcc acgtaataag taaagaaacc tggcaattta
    35761 acaaagttca tagcaaacat agcaaatccg taaatcagta aaaaaatcag ccctgaaaaa
    35821 agattttgag acaaaagata gatagttaat cgctttccct tttttccttt ctctcttgct
    35881 ttattcgtga gctgtggatt aacatataaa agattaactc gaattaattc taataattgt
    35941 cgcttataca tacggtccat ctcctgtcgc ttgccttcca gccattttca aataaatctt
    36001 ttctagcgtt tcatcaggtg agccattcaa taattcacta actgccccct gataaatcaa
    36061 ttcacctttc ttcaaaatag ccagtttatc gcataactgt tgtgcggtat ccaatacatg
    36121 tgtagaaaaa atgactgttt ttccttttgc ggcatgagcc ttcatcattt ctttcaaatc
    36181 aaatgctgct tgaggatcaa gtccttgcaa aggttcatcc aatacccaaa tatctggatc
    36241 tggcagcaac gtgccgatca aaatcgtttt ttgacgcatt ccatgtgaaa aacttgcgat
    36301 tgtttcattt tgatgactat acatatcaaa aagcgctgtc aattctgcta aacgcttttc
    36361 ttttttttgt gtgtctaatt cataggcagc tgaaatcaag tcccaatatt ctccagccgt
    36421 caattgtaaa aaaatatctg gggaatcagg cacataaccg attttttgtt tgatactcaa
    36481 tcgattttcc gtcaatttca ttccatctac taaaatcgtt cccgatgatg gttcaataat
    36541 actaactaaa cttttaatcg tagttgattt tccggcacca ttatgtccta aaaatccaaa
    36601 aatttcccct tgtcttatcg agagagacaa gtcttttaat gcttgttttt tcccgtaact
    36661 tttacttaca ttgatgaatt caatcatttc tgtactcctt ttgtttgtta ttacttttta
    36721 ttgtaccctt cgttattggt ggagtaaatg tcaaaaagag tgcataacaa aaaaggatag
    36781 aaacgactag gctgctactt gggttgttct cttctacagg aaaacactct tctaacaggt
    36841 aagatatgag tctttgtttt ctttaaaatg attcattttc tatcttttct tgcataaaat
    36901 tctttagttt atcaatactt ctctttccca tctctagttg ttctccattt gcaaatgtta
    36961 ctatttcgtt tactctgcta atagatgtaa tgttttcagt attaatgata taagctttta
    37021 aatctttaca taaatactca tattcgagtg attttttata ttttttaatt gtgccgttta
    37081 cgagaatctc ttcatctttc aatactaggc ctattttatt tttataacct ggcatgctca
    37141 ttagataaag gatttcatcg taatggatat agtgatcgct attaccataa cggacagttg
    37201 tccacctttt tttcgtagca tgttgaacag gatagttttc tttttctttc aataagtaac
    37261 tttggatgct ttttttattt ttttcataag tgaattcttt tatcaaataa ccactaggcg
    37321 taatctttcg attaataaca gttattgctt tattcggatg agacgttaga taaacaattt
    37381 tacacttttc attcttttct ctaagcattt cgcctagttc gataccgcta taataggcgt
    37441 tcaaatcgat gtctaggaaa aaaatatctg aggaatagat ttcattattt ttaatgaatt
    37501 caacaaattg tataacgttc aatccctgaa cgatctcaaa tttccaagga cttaataaaa
    37561 ttctggattc ttccaaaatt tctactagca ttttttcaaa aaccaaacta tcatccaaaa
    37621 taaatatacg cgttaccata aaaagtacct ccactcgata ttttactatc aaaatcattt
    37681 catacaactt ttcaacatac atgttaggaa taaattgtaa ttctcttcac aatcttttcc
    37741 aacacctttg aaaccgatat caaatacttt caggatgctg cttctaaaat aaatatcaaa
    37801 caaatgaata catcatgcaa aaaaagccag acaaccgacg tccttgcgtg tcagctgtct
    37861 gagctatgtt tatttatttt gttcttttaa aaagaacgat acacatactg atttttcgga
    37921 gcagagactt tctcagcgtt tgtaacaact acagatggga tttcacgctt gaatggttga
    37981 taatagtctg attggatggt tccttctatt ttataccatt catcattttt aaccgttact
    38041 ccttccggca tatgcgtcaa caaaccaaag actcctgaat ctgccacaca atggataatg
    38101 ccgaatcgga acaagaagat atctgtattg ctatcgttcg gtgtttggta aacaaatcct
    38161 tcatagctga tttttttgcc aatgaattcg ctagggtaat tataaattag ctccataact
    38221 tctaaatagt tttcatctgt gatcttgata gtttgcttat ccccatactt ttctttcaac
    38281 tcattcattt ggtctaagta gtctgatttg ttgaaataaa tactggtatc cggctttaag
    38341 tattgtgtct gcatctccgg atcccctact gattctttac ttactgggaa attaaatcct
    38401 ttggcttcta cgatcgtagt gtcgagacta actgttggaa acaacgtccc cacgattact
    38461 ggcaaagcta gcagtacata agcgattcca cgttgataag gttttgacaa cccatggtca
    38521 tgatcgtgct cgtgtgcctt ttcttctgct tttccgtttt tgacccacaa atacagctga
    38581 actaaagcca aaagagcaga taaaatcatt gaaagaatag ctaagtagcg ataatggaca
    38641 ttgatatatt gatcgagttt gccagaaact tgcaagtaca tcatcaattc gaaataccca
    38701 gataaaatca aaaaccgcag catattttcc ctcctaaata aataatgtga atccaataac
    38761 gctaagggca atcagtccga tcagcgataa aataaagcgt cctgtaaaat atcgtttcat
    38821 catcagtaga tttttgatat cgaccattgg gccaaagacc aaaaaggcag ctacaggtgc
    38881 tgtgccgaac agacttaaca aagaagatcc tataaacgca tctgcttctg agcaaagaga
    38941 catcgtaaag gccaataaca gcatgatgat aatggctaaa atctttgttg agccgagcgt
    39001 tagcataatt ctagttggta aataagtctg catcgcagct gcgattaatc cgccaatgat
    39061 caaataacga cctgtatcaa aaaattcgtc gattgaatgt gtcaacacat gcccagtttg
    39121 atggatcagc tgattttttg gttcattctt ctcactttgt tcttcttcat ggacatgatg
    39181 atggtgattt tctgctactt tagacgtgat tttttctttc aatacagcgt ctttttgaaa
    39241 ataggcaagc cagacaccaa tcacaagggc aacaaaaaag ctgcctatca cccgccagct
    39301 tgccatctgc caagaattgc caaatgcgat aaaagtggaa aaaatcacga ttggattgat
    39361 gatcggagca gtcagcataa aagcaaacgc tgtgtgaact ggtacgcctt ttttaacaaa
    39421 ttgatgaacg atcggtacga tgccgcattc acatgaagga aagaaaaatc ctaagatcga
    39481 accagtaaaa atagagaaga tacgattttc aggaagccat ctcttcacac gttctggggt
    39541 caaaaatact tgtaaggcgc ctgagataag acagcctaat aatacgaaag gtaatgcttc
    39601 aataacgatc gataagaaaa tcgtactcat ctgcaaaacc gaatttggaa taaaagaaaa
    39661 catgagtttc ctccttttta tttctagttc attcgctttg caaatatacg cttttattca
    39721 aaaaaaacaa gttgctctgt aaatttttac acaactctta ttttttacca aagcacttca
    39781 attgactttt tctgttattc ccattaaatt aaggtctcat acatatcact tctatcataa
    39841 aagggagaat aaaaatgaaa caaaccacta acttgactga agttcaagac atactgcaac
    39901 agtcatccgt tgccgtcatt tatctttcga tgcctaattg cagtgtttgt cacgctgttc
    39961 gtccacgact agaggaactt ctcacacatt atgatattcc ggcacttcat ttggatgcat
    40021 ttgaaacacc agaagtagct agtaaatttg aagtgctgac tgcgccagtt gtcctgattt
    40081 ttcatcaagg aaaagaagtc tttcggcaag cacggttcat cgactttaaa aaaatccgtt
    40141 atctactaga tgaactgaca gcagaagatg attccctttc ttatgaagaa atcttccgaa
    40201 caaaataatc gttgtccaaa cagaaaaaag cgattgaaca agtgaaagtt ttgccttcat
    40261 ttgttcaatc gctttttatt tttattctgc tgtattttcg gaatgcgcat ttgcgtcaga
    40321 atgcaagaaa tagatcaagg tttgcaattc attcgtcaaa tcaacatttt ggatcaatac
    40381 atctgacgga gcattcaatc gagcagcggt aaagttcaag attcctttga ttcctgcttc
    40441 cgttaactga ttgactactt cttgtgctct ttcagcgggt atcgttaaaa ttgctacttc
    40501 gatttgttgg atcttgattt gttctgacat atcttccatt ggataaacgg ggataccgtc
    40561 tacgatgcgc ccaacgattt cctcattcac atcaaaagca caactgatac gtatgctatt
    40621 actttgatgg aatttgtatt ttagtaaagc acttcccaag ttccctacac cgatcagcgc
    40681 aacatttgtc aattgatcgt cgtttaatgt ctttgcaaaa aagtgcataa gatcttctac
    40741 atcataacca tacccacgtt ttcctaattc gccaaaataa gaaaaatctc gacggatcgt
    40801 tgcactatct acttgaacgg cttcacttaa ttcagttgaa gacacttttt tctttccagc
    40861 attatttaaa atccttaaat agcgataata caaaggcagt cttttagctg tcgcctttgg
    40921 aataatatga tctttcacga aatgacctcc acgtattgtc taattacatc tttgatttct
    40981 ccatcatagc agtatttata ggaaaaaatc aattccattg ctcagatatt gtgaaatttt
    41041 attctttatt catttattta tcatttctgc aaatctcgca gtataatgga atttatggta
    41101 gactactaat tgaaaaatag ttttttattt gaggagaatt cacaaagtag aaaagaggat
    41161 tccatgatat tattacaagc caatcagatt gctcgccatt ttggtgcaga agtcttattt
    41221 gaaaacatac atttagaaat cagtacaggc gctcgtgtcg ctttagttgg cagaaatggc
    41281 gctggaaaat cgactttatt aaagatcatt gctggaattg aagcacctga tgaaggaaag
    41341 atcgctaaaa ataaaacagc gactctcgga tacctcgctc aggacacagg acttgattcc
    41401 aatgaaacag tttggaacga gatgctgaaa gcttttgaac aagttagaca aatggaagag
    41461 cggatgcgcg agctagagat tttcattagc gaaggtacgc cagacacacc tgcttatagt
    41521 tcttatttaa aagaatacga ccggctgcaa cacgattttt cagatcgaaa cggctatggt
    41581 tatgaaaatg aaattcgttc cgtattgcat gggtttaaat tcgattcttc attttatgat
    41641 cagaaaatcg atactttatc aggcggacag cgcacacgtt tagctttagc tcgcatgctt
    41701 ttgcaacacc ctgacatttt gattctagat gaaccaacga accatttaga tatcgagaca
    41761 ctttcctggc ttgagaatta tctgcaaagc tatactggtg cattattgat tgtttcccat
    41821 gaccgttatt ttttagataa agtagtcaat gaagtctatg aattaagcag aagaaaaatg
    41881 cgccattaca aaggtaacta ttcgaaatat ctagatttga aagcagaaca gttagcaagt
    41941 gaatggaagg cttatgaaaa gcagcaaaca gaaatccata aattagaaga cttcgttgct
    42001 cggaaccttg ttcgcgcctc taccacaaaa cgagcgcaaa gccgcagaaa gacattagaa
    42061 aaaatggacc ggctggatcg tcctcaaggg gatgaaaaac aagcaaactt tctattcagt
    42121 atagaaaagc cttctggaaa tgtagtcttg caaatcgaag atgccgctat cggatatgat
    42181 aatgtcacct tatctgaacc aatcaatcta gatatccgtc gtcaagaagc gattgctcta
    42241 gtaggtccga atggtatcgg gaaatcgacg ttgctgaaat caatcattca ccagatccct
    42301 tttatccgtg gaaaagaaac gctagggaca aatgtcacca tcggctatta cgatcaaggt
    42361 caagctgatc tgcatacaaa caagacgatc ctagcagaat tgtgggatga gcatcctacc
    42421 actccagaaa aagagatccg aagtgcttta ggtgctttct tattttctgg cgaagatgta
    42481 gaaaaaccaa tccctctgct aagtggcggt gaaaaagcac gggttgcttt agccaagtta
    42541 gcgatgaaca aagaaaattt tttgattttg gatgaaccaa caaaccattt agatattgac
    42601 aacaaagaag tattagaaaa tgcattgatc gattatgacg gcacactgct ttttgtttcc
    42661 catgaccgat atttcatcaa tcggatcgcg acaaaagtcg tcgaactctc tgaaaatggc
    42721 agcaaagtct atctaggaga ctatgattat tatttagaga aaaagcagga agaagaagaa
    42781 atcgccgaat tgttggcaca agatgagcca gaatctgctc ctgctcccac gaacaaatct
    42841 gattattacc aaagcaaaga acagcaaaag cttatccgta gccttcaacg aaaaattact
    42901 caggttgaag aagaaatggc gcggatcgat gaatggatcg atcagttgga acaagaaatg
    42961 gtcgcgccag aaaacctgga agatcacgta aaactcaatg agctgaacca agatttagaa
    43021 gctgctagac aagatcaaga aacaaaattg acggaatggg aagaactaag cttggaatta
    43081 gaagaatatg aaaattaacg aaaaaaccaa aacagaaacc attcatgctg ttttggtttt
    43141 tcttttttta ttttatcaaa caaaaattcc atatttacct ttttcaccaa taagaagacc
    43201 aattactagt cattctcact aaagcttcta aataatagta atcaccccaa atacaaggtg
    43261 tatttacccc attatttttg ggcttattat aaacagactg tgttaaaaag ccatcttctt
    43321 cttccgttgt agcatatctt tctttcaatt gtcgcataat tttccaaaca gcagactggt
    43381 ataagttctt ttctgttttg tctaatagac ctgattctat taattctaat aatccacaaa
    43441 ccatgatcac catactagat gtatcttttt cttctctgct agaatcatca aagatcaaat
    43501 cccaataaca aatggtatct tctggcaacc gattcataaa ataattggct aatattttgc
    43561 ttaaatcaaa atatttttgt tctttcaaat aacgataact taatgcaaaa ccgtaaatcc
    43621 cccatgcttg gcctctagcc caacaggaat tatcactata accttgatgc gtacttccat
    43681 atcttggttt tccatctttg acatccatat aaaatgtatg ataagttgaa ccatcttctc
    43741 ttactaaata atctgctgca ttttgaatat gagcctgagc agctttttta tatttttgat
    43801 ttccagtttc ttcactcgcc caatacaata atggaagatt catgcaacaa tccatgatca
    43861 ttctaccttg ttgtgaagga tcactaggat ctccccaagc ttgtataact cctgccttct
    43921 cccaaaaacg ctgaagtaat gtttctgcag catcaattga agcttccttc atttttcgat
    43981 ttcccgttat tttataatct gctacggttg ataaagaata caaaaaacca agatcgtgat
    44041 gattagtgat aatttgacag tcaatcctgt ggataaaatc tatatctgct ttttcagctg
    44101 tttgttgaaa taaaggatca cccgtcttct caaataacaa ccaattaatt cctgtccaaa
    44161 atccttctgt ccaaccaata ttgtctctta atggataacg tccttttata ggacactcgc
    44221 ttgggaattg atactgactg ttactgatac gtttgattaa cctctccgtg tgtctgcgac
    44281 aacgctcgat ctcattattc cattcttttt ccattaattc tttataacat tcatttatcc
    44341 atttttctgg atgtattatc tcttcaaaca tcactttatc aagcatttcc ccaccagcct
    44401 ttagcataat tttagtcttt ttttattgtt tttattttgg atagttagcc cggttgtccc
    44461 atcaaaatca attttgggac gattgttcca tacttctaaa ctataatccc gatcttgatg
    44521 agcaaccact cctgttccga gccaatgggt tccttttcca aatgtaccct ctaatccagg
    44581 aataaaagtg ttttcccatg tcatcaagtt tgtattgggc aaacccataa tagctgtgga
    44641 ttttcgttta atagtatcgt cacttaaaca gataatacct gaaaagccag cttcactcac
    44701 cctatagttt tccttgtctt cttctatgat tattccttct ttctcaggag aacattcaat
    44761 agcaaaccct gtctcatata gaacatattc tgtatctaat tctactttgt gtacgcgaat
    44821 ctgccagcct tcaaaaggaa taagccaagt tctgattttt gcctgtaaag taggttccca
    44881 tgtagaggca agtacctctt tcttacatac aatgtctcgt acattatttc gaacgatgaa
    44941 ctgtccatag gttttgatgc tctcattctt aaaactggtc gtgatatatg tgtccgctcg
    45001 ctgaatccct agcattgaat ccattgccat ttctgctttc aattgattcg ctcgcggtat
    45061 cgaaaaacca aattgactag aataagcaaa ttttaaatat ttttcattgg tcaatgtatg
    45121 gaaatttggc cctaattgtc ccgcattcaa aaaaaatgta tgtccattat catgttgtat
    45181 gagcaaattg gcttctgaaa gaagtttagt tccttcacga ataggcatag gcatttcttt
    45241 agccgtccaa tactcatggt cttcaggtag cgccaacaat aaaaaaatct tattcagcca
    45301 taatggagat aacgtactat tatagggttc agtcaatgtc aattgagggt aggtataacc
    45361 aatattcagg ataccttttt catcaaatat ttctttttgc atccaccatc ttatattccg
    45421 attgactaac cccttgattt cacctaattt aaaaggcaaa agatcggccc acaacatagc
    45481 tgaccaaaag cttgaaactg caaaacgata aatcatgctt cgaccaaaag gcacatttgc
    45541 accatcttcc gcaaaaaaat gaatatagtc tctagaaaaa tcaatagctc ggttcttata
    45601 tatctggcta atgggctttt ctggttctag tacgctataa atcaaaccat aataatgaaa
    45661 tgcaaaagga ttataataat cttggcgacc tcttgaactg tctcgatacc agccattttg
    45721 aagataacaa ttttctatat tatcaaatgc ttctttcagt gctgattctt taactggata
    45781 accaaatttt tggataattg ttccgacaat tatcttaaaa aattgccaat taccatctgc
    45841 acattcatat tcaaaaattt gcgagaacca atgaataatc tggctttttt ctcctgtatc
    45901 taattttttc cataataatt gttcatgatg atataacgct aataacattg cgggcatttc
    45961 taccatacgc tggtctcttt catgtatttc tccccaataa tcgggatggg aaggatctgt
    46021 tccagctttt attcctgata gaattaattt caaccatcgt tcatctaatt ggtcaattgc
    46081 gggccccgct ccccataaaa cccgagagaa tccttccatt cctgcaattt tatttccata
    46141 tcctacccct tgatgtatat aatcaatttg tgcgccactt gctcgataaa acggctggag
    46201 tggttgattt atctttttaa ataagtcaaa gtaatcctgt ctggtcctta atagtggtac
    46261 tggatgtttc attgtatttt ctcctctttt tactaccaaa tagctaattc cgactgttct
    46321 aatcgaagta agccttcaat aaaaaaataa tcagaataga tcagtggcac attataagtc
    46381 agttctctat ggtatgcttc tgctccgcct ccaatgatcc catcttcatc catattccaa
    46441 tttgcatagt gcttttctgt cttttggagt atattgatag cagcttcatg atacattttt
    46501 gatcgtacag aggaaacata tcctgctaat tccagaagac cgcatgcagc gcatactcca
    46561 gcactggtat caatattcga atcttttaca gcaaaatcaa tgagaggaat ataaccagtt
    46621 ttttcgattt tctgaataaa atttactgct atcttttctg ctatagctag atagtacgga
    46681 ttttttgtgt aacgataact taacgcgaat ccatataaag cccatgcatt tcctctgctc
    46741 caacaagaat tctcattgta tccttgcccg ccaagtaacc gaatatattt ccctgattct
    46801 gcatcaaatt ctgcgatatg cccggttgat ccatctttcc tcactagata cttagctgtt
    46861 gtatctgcat gctgccgagc aattttagag aacctcggat caccagtttc cctcgaagcc
    46921 caatacagta atggtaaatt catcatacta tcaataatca tccaaccagg tttatggtca
    46981 ttccatgcta ctaaatagtt ccctgaaata ttatagcgtc cagcaagtac cgtagctgca
    47041 tgtaacccta gttgtcgact atgtttgttt tgagactgtt gataatgtgc aatagccgat
    47101 ggtaagaata aaaaacctac atcatgatga acgtctgaaa aatggtacag ggcagattct
    47161 aataattgtt cttgttgtat tgctgcctct ttgaactttt ctcgttttgt cttgtgaaag
    47221 agctgccata aaataccact ccaaaaacca ttggtccacc agtcgattcc tctgtccttt
    47281 attacgtctg tatattttcc gttgtaagaa atatatggaa ttttattccc cattctgtct
    47341 aattccgcat ctatcttttc agaagctaca ttcaaaattt ttcttgacca ttccttcgtc
    47401 tgttcattca tggatatccc gccattcttt taatttttat gaatgtggag ttgctcctag
    47461 aacgaactag atggttaaca ttcgttattt tttttactta tttgcagctc caatgtcatt
    47521 ctccttaaat ttagactgcg cccacacata aatcaagacc attgggatca ctgaagttgc
    47581 taaaattagc ataattgcgc cataattaat tggctcacct gtactagaaa ccgtcatatt
    47641 tttgataatt aagggaaccg tatatttatc tttatctgaa agcatgacta atggaaatag
    47701 atattgattc caacttccca taaagataag taaaccacta gtaataatcg taggtgataa
    47761 aatcggtaca gcgattttcg taaaaatcac ccactcattg gcaccatcga ttctggcagc
    47821 ctccatcagt gctgtaggaa attgactgct atattgtcgc atcataaaca ctgaagttgt
    47881 actggcaatc gaagggatga tcacacctgc ataagtattc gataatccta aacgactcat
    47941 aatgtaaaat aaaggaatca atgtggtaaa aaaaggcacc atcctagata gcataactgt
    48001 attgaacaac agtttattcc ccataaaacg atactttgct aatgcataac ccgccattgt
    48061 agaactggct gtagctaaaa ctgtactgat tacagcaata acaatcgaat tccaaataat
    48121 caagggataa ttatatctcg ccatgatggc ttgccagttt tttaatagca tatccccaaa
    48181 cttgaaattg attccgccac ttaaaatttc ttgattactt tgcgttgaag ctacaatcat
    48241 atataaatat ggcatcaaaa atattaaggc accaaacaca ataatgacgt acgtaaaaat
    48301 tctgctttgt atcttttgtt tagtcatttt tatcacctcc gaatttaaat tgaatagccg
    48361 ataatataac agatacaaac accatcgtcc aggcaatagc agaggctttt ccaaaatcaa
    48421 ccatttcaaa acttgtttta tataaattga ctcctacgac caaagctgaa ttttctggtc
    48481 cattggaagt cccaaagaat atatttggga tattgaaatt acccaagcca ttgatagtgg
    48541 ctaaaactgt ggccattaaa atgattggct ttagcatggg gatcgttaat aaaaagaatt
    48601 gccgaaattt cgatgctcca tctatttcag cactttcgta tatctcttta gaaatccctt
    48661 gaagaccagc taaaaataaa atagtgttat atcctacact aagccaaatc atcgtgatga
    48721 tcattgatat tcgtatccaa aaaggatttg ttaaccactg tacaccgtta attcctagcg
    48781 cattcaaaaa tccattgaat aacccagtcg gattgaaaaa agtcatgaat acggaagcta
    48841 cagccaccgt tgatgtaatg gttggaatat aatagacagt tctaaaaaat ccttttcctt
    48901 ttaactgtgc tgaattcaat aaaacagcaa agtaaatagc taatagcatc gttattggaa
    48961 ttgatcctgc catgataatc aaaacattcg tgtaagattt aataaacaat ggatctcgga
    49021 agatagctag ataattgtca aatccaacaa attcaaatga gtagccaatt ttcataaaac
    49081 taaaagcaaa cgagataatg atgggaatca aaatcgtcac aataaagaaa ataacaaaag
    49141 gtgtgaccaa tccataagca ctttttttat gagacgtatc tgttgctttt ttcttgctgt
    49201 tctttattgt cttgcttggt aacgaagatt gcatcatcgt taagtcctcc taatattttt
    49261 taatcaccag ttaattttac gccgtactta tgagcaaaat tttctgcttc ttttagatat
    49321 gaattttcaa aatcacctgt ttgccaatag ttatatgcag cggtagtcaa ataagacgta
    49381 gcatctgaat aagtggctgg taaaacagta tttgatgtat tttcattgat tttattcaat
    49441 agttcatagt atttttgtcc tccttagtag tcaaactttt tctcagctgc ttctgtttga
    49501 taggccaatg agttagatgt agccacgccc agctccagtg cgttggctaa gcattcttca
    49561 tctgtcagca taaatgtaag aaattgttgt gccacttgtg gatttttggc atctttaggg
    49621 acataaaaac ttgatcctcc tgatactgca ccttttcctg gattttcttt tgaaaaagga
    49681 atcagtggag ccatgcgcca tttgtcagat gcatcaggaa aattcaaaga catattcgta
    49741 gcataccagc cgccagcaac aaacattgca gattcttgaa atgctttctc agcatcttgt
    49801 gcaccgtaga aatgtacaat atctgagtcg atcatttctt tgattaactt tgcagcatgt
    49861 ttcgcttctt tagtattcag acggatattt ccttcttcat caagcaaaga ttgattctgc
    49921 tgggctagca tgttgataaa caattcttgg tccccagatt ctggcaaggc aattccaaaa
    49981 ctaccagttt tttcttttaa tgcttttgcc acctggatat attcttccca agaactgatt
    50041 tcttcaaaat ctacacctat ttcagcaaat ttttttggtt gataataaca aagtgctggg
    50101 gtaaaatcac tagcccaagg aataatcgtt tcattactcg tttgttgacg caaaatattc
    50161 aatttttgat cataaaactg atcgccatat ttgtcataaa atccatattc atcagcacta
    50221 taaaaagcat cttgaaattt ttccagcaca cctgctgctg aagtgtctgc catggagata
    50281 atatccggca tatcttcgtg tgttaccaat tttggggtga tcatttgtac tgttgctgtt
    50341 cccgaagcta cattcacaat ttttaagtca atatttgctc cagttttgcg gttatagatt
    50401 tttgcagcat tttcaacata gtttgtcagt ttgccgtttg cgacatatac ttctaatgta
    50461 gtggcttttt ttatcattgg atctgcgatg tttgctgtta cttcttcgtt agtttttcca
    50521 catgcactta agctgaataa tagggtaatg tttagcaaag ctatcaccaa tttgtatctt
    50581 tttttcatgt ctatcccttc tttcttttca agttcttata taaaacgctt tcacttatgt
    50641 atattaacgg aattagtaac acaaatattt atataaaaaa atattttact tgtcgttttg
    50701 gcttttcatt tttgttataa tagtcattaa ttataaatag aaagcttttt atataaaaag
    50761 agaaaggaaa gtggttcttt tgttaacaaa caaccaatcc tccaatcttg tcaaaaaaac
    50821 actaacctat tccatagata cccatcttca tttatctgct gctccctatt ttcgactact
    50881 aataaatgaa agcgctaatt tatatgctga atttgcacat aaatctatag aactagataa
    50941 tccttccctt ttattcattg ataaacagca agaagtccaa atcttttcta ctcaaaagga
    51001 aacacaaact tttatcatta tccagtttta ttttgatgag caattattag cacatattac
    51061 gggacaatct ttttctatag atcaagaaat aacatctatc attacttttc tgaaaaatga
    51121 acagttcaaa attgattatc taaaacatag taagccagct atttcctatg atcaggaaat
    51181 caattctttt ttcatactat gccaacagat gagcattcat ttgctttcct tattagttac
    51241 ttattgtctg caaaaaattc aattgtcttc cttaccaagg cattatcaac aggaacttgc
    51301 tttattgaca ataccttcaa atgagaaagt cattcctaaa aaaaagcata ttctttattc
    51361 agatgaatta gttgatccaa tcatacgata tttagaagaa catctttcag aaaatttttt
    51421 gcttgaacaa ttagcccaaa ttttttttat cagtccagcc agcttaaaaa aacattttag
    51481 aaaagtgaat ggaaaaagtg ttattagcta ttttaaagaa ctaaaaatgg aaaaagcaaa
    51541 acgtctcgtg ctagaaaatg aattaaccta tacagaaatt gcagatatat taggatttca
    51601 ttcgatccat catttttctt cagcttttaa aaaatataca gggttatccc cttcaaagta
    51661 ttatctatcc ttaaaataga aaaaaatttt taggttttat ccacaaatta tttctctata
    51721 ttttctgtct attcaaaata aacaataatt ttccggcaca attatttgct gttttttacc
    51781 ataacatagt acgatcaacg tagaaaagga gatgttttta tgaaagttgt tgtcattggc
    51841 ggtgtagctg gcggtccttc atttgccact cgtttccgtc gattgaatga agcgcacgaa
    51901 atcattatct atgaacgcgg agaaaatatt tcttatgcaa gttgtgcttt gccttattat
    51961 ttaggaggtg tgatcactga ccgtgactcg ctgatcgaac gtacaccaga aatattgaaa
    52021 acaaaaaaca acatcgatgt ctttactaaa cacgaagtaa cagcaatcga tccttctact
    52081 aagcgattaa cagttaaaga cctatccaca aatgaagaaa taaaaacaga ttacgataag
    52141 ttgatcatct cttctggtgc tagaccagat tatccggata ttcccggagt ttttgaagca
    52201 gaaaacggct ttgtcctccg tagtgtgacg gatgcggatc gaatcaaatc gttccttgaa
    52261 gaaaaaaatc cacaacatgt cgtcattctt ggtgcaggtg ttatgggtct ggaattagct
    52321 gagaatttca agcatcgcgg cttagacgtg actttaatcg atcaattgcc acaagtcgct
    52381 ttcccttatg atccagaaat tgctaattta gtttatgaca aattgctgaa agaaggatta
    52441 gccgttcatt tagaaacaag agttactgag atccgtgata aaggtcgaga aatagtatta
    52501 tcagatggct ccgtcctttc tgctgatatg ctaatttttg ctgttggtgt ttctccgaag
    52561 aatgaagtgg tgaaagcaac cggcatacaa ttatcagata caggtcagat cattgttgat
    52621 gaccagttac aaaccaatct gccggacatc tatgcgattg gcgatattat cgaaacgact
    52681 agtgtagtga ctggtcagcc gatccaaagt atgctttcca gtgcggccaa tcgtcaagga
    52741 catatgctgg cagatatttt aaatggtacg cctatgcgct atcgcggata tattggtgca
    52801 ggtgtcgcaa aaatctttga tcatacagca agttatgcgg gaatgacaga acatgaatta
    52861 aaagcatcag gcataacaaa ttataaaact gtttttatca ctccctttga ccatgcctat
    52921 ttctatccag aagctacaag attaaatcta aagctgattt ttgatgcaga tagcggtcgt
    52981 attttaggtg gacaagcatt tggagaaaaa ggtgtcgata aacggatggg agaactttct
    53041 gtagcaatca ccggaaactt gacagtcttt gatttgcctg atttggagtt gccttactct
    53101 ccaccgtatt ctactacccg tgacccgttg aatatagctg gttatgtcgc aatcaatcaa
    53161 atgacgaata tcgtagaaac gatcaaagca agtgatatac ccgaaaacga tttgaaagaa
    53221 gcgttctttt tagatatacg tgaacctaat aaagcacctt ctggcagtat ctcggcaaca
    53281 aaaaatattc caatgaatga gttacgagat cgaatcaatg aaatcccaaa agataaaaaa
    53341 gtttacatta ctttcagaag aggattgaat acttatactt ctgcccgaat cttggcaggt
    53401 ttgggtatca aagcgatttt gattgaagaa taaaaaaacg accgcggtgg gtagccgcgg
    53461 tctaggagtt atttttactt gaggagtaaa aatgaaaaag tatatagggt tgttggttgg
    53521 tatgtatata gaatagaaga taaatgtgaa gaaattgtgc attatttctt cacatttatt
    53581 aggttatagt ttaagaaagt ttaaacattg cgcaatttaa ttattttagc tgtttctttt
    53641 cataaaaaaa catgttgtga ctctgagaca aatcatagtc catagtgatg tctctagagt
    53701 aactacgcct aatattacga ggcatcatat taatctatca gcactagtac ctaaactagc
    53761 atccctttcg aatatttttt cttatataat aaagtttgct ttcactcctc taatctcacc
    53821 aaatcgactg gcttgcgttg tgtgatacta ggaactgatt ggtttagaac gacttttcta
    53881 ttgtaatcat atttttcgcc atcctcgttg tcttctaatc cttcaatgaa tgatccctaa
    53941 tgtgtgtatt aaataattta atttttttgt tcctatttat ttaa
//
LOCUS       DARBFD010000008        51343 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000008 DARBFD010000000
VERSION     DARBFD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.008"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            330..1175
                     /locus_tag="SCK08_000486"
     CDS             330..1175
                     /locus_tag="SCK08_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1200401.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="SCK08_000487"
     CDS             1456..2676
                     /locus_tag="SCK08_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEG1200402.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="SCK08_000488"
     CDS             2673..5393
                     /locus_tag="SCK08_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1200403.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="SCK08_000489"
     CDS             5377..6321
                     /locus_tag="SCK08_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1200404.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="SCK08_000490"
     CDS             complement(6390..7400)
                     /locus_tag="SCK08_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1200405.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="SCK08_000491"
     CDS             complement(7580..7924)
                     /locus_tag="SCK08_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1200406.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="SCK08_000492"
     CDS             complement(7917..8351)
                     /locus_tag="SCK08_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEG1200407.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="SCK08_000493"
     CDS             8489..9226
                     /locus_tag="SCK08_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1200408.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="SCK08_000494"
     CDS             9219..10430
                     /locus_tag="SCK08_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200409.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="SCK08_000495"
     CDS             10634..11416
                     /locus_tag="SCK08_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEG1200410.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="SCK08_000496"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="SCK08_000496"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEG1200411.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="SCK08_000497"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="SCK08_000497"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEG1200412.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="tadA"
                     /locus_tag="SCK08_000498"
     CDS             14132..15061
                     /gene="tadA"
                     /locus_tag="SCK08_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEG1200413.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="SCK08_000499"
     CDS             15018..16067
                     /locus_tag="SCK08_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEG1200414.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="SCK08_000500"
     CDS             16064..16342
                     /locus_tag="SCK08_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEG1200415.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="SCK08_000501"
     CDS             16317..16787
                     /locus_tag="SCK08_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEG1200416.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="SCK08_000502"
     CDS             16804..17106
                     /locus_tag="SCK08_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200417.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="SCK08_000503"
     CDS             17123..17494
                     /locus_tag="SCK08_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEG1200418.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="SCK08_000504"
     CDS             17466..17825
                     /locus_tag="SCK08_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200419.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="SCK08_000505"
     CDS             17997..19004
                     /locus_tag="SCK08_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1200420.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="SCK08_000506"
     CDS             19029..20213
                     /locus_tag="SCK08_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEG1200421.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="SCK08_000507"
     CDS             complement(20650..22233)
                     /locus_tag="SCK08_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1200422.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="SCK08_000508"
     CDS             complement(22811..22954)
                     /locus_tag="SCK08_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEG1200423.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="SCK08_000509"
     CDS             complement(23176..23655)
                     /locus_tag="SCK08_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1200424.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="SCK08_000510"
     CDS             complement(23728..24012)
                     /locus_tag="SCK08_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200425.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="SCK08_000511"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="SCK08_000511"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="SCK08_000512"
     CDS             complement(24356..25642)
                     /locus_tag="SCK08_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEG1200426.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="SCK08_000513"
     CDS             25780..26451
                     /locus_tag="SCK08_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEG1200427.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="SCK08_000514"
     CDS             26417..26905
                     /locus_tag="SCK08_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEG1200428.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="SCK08_000515"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="SCK08_000515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEG1200429.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="SCK08_000516"
     CDS             27866..28630
                     /locus_tag="SCK08_000516"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEG1200430.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="SCK08_000517"
     CDS             28863..31076
                     /locus_tag="SCK08_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEG1200431.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="SCK08_000518"
     CDS             31087..31533
                     /locus_tag="SCK08_000518"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEG1200432.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="SCK08_000519"
     CDS             31704..32963
                     /locus_tag="SCK08_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEG1200433.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="SCK08_000520"
     CDS             complement(33052..33999)
                     /locus_tag="SCK08_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1200434.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="SCK08_000521"
     CDS             34393..35685
                     /locus_tag="SCK08_000521"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEG1200435.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="SCK08_000522"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="SCK08_000522"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEG1200436.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="SCK08_000523"
     CDS             37578..38453
                     /locus_tag="SCK08_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1200437.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="SCK08_000524"
     CDS             38719..40602
                     /locus_tag="SCK08_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG1200438.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="SCK08_000525"
     CDS             complement(40677..40961)
                     /locus_tag="SCK08_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1200439.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="SCK08_000526"
     CDS             complement(40951..41685)
                     /locus_tag="SCK08_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEG1200440.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="SCK08_000527"
     CDS             41814..42437
                     /locus_tag="SCK08_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEG1200441.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="SCK08_000528"
     CDS             42629..43750
                     /locus_tag="SCK08_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEG1200442.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="SCK08_000529"
     CDS             43757..46885
                     /locus_tag="SCK08_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEG1200443.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="SCK08_000530"
     CDS             46904..50098
                     /locus_tag="SCK08_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEG1200444.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="SCK08_000531"
     CDS             complement(50244..50951)
                     /locus_tag="SCK08_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1200445.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16814 a         8955 c        10648 g        14926 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acagtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttctgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac
    51241 tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt tatttagtaa
    51301 tcattgcttt aaaatactac gcttatataa cagagaaaat aag
//
LOCUS       DARBFD010000009        49184 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000009 DARBFD010000000
VERSION     DARBFD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.009"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            123..935
                     /locus_tag="SCK08_000532"
     CDS             123..935
                     /locus_tag="SCK08_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEG1200446.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            932..1732
                     /locus_tag="SCK08_000533"
     CDS             932..1732
                     /locus_tag="SCK08_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEG1200447.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1893..3161
                     /gene="rseP"
                     /locus_tag="SCK08_000534"
     CDS             1893..3161
                     /gene="rseP"
                     /locus_tag="SCK08_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEG1200448.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3229..4938
                     /locus_tag="SCK08_000535"
     CDS             3229..4938
                     /locus_tag="SCK08_000535"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEG1200449.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5150..9508
                     /locus_tag="SCK08_000536"
     CDS             5150..9508
                     /locus_tag="SCK08_000536"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEG1200450.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9653..10126
                     /gene="rimP"
                     /locus_tag="SCK08_000537"
     CDS             9653..10126
                     /gene="rimP"
                     /locus_tag="SCK08_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEG1200451.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10149..11324
                     /gene="nusA"
                     /locus_tag="SCK08_000538"
     CDS             10149..11324
                     /gene="nusA"
                     /locus_tag="SCK08_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEG1200452.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11346..11639
                     /locus_tag="SCK08_000539"
     CDS             11346..11639
                     /locus_tag="SCK08_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEG1200453.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11636..11947
                     /locus_tag="SCK08_000540"
     CDS             11636..11947
                     /locus_tag="SCK08_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEG1200454.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11960..14266
                     /gene="infB"
                     /locus_tag="SCK08_000541"
     CDS             11960..14266
                     /gene="infB"
                     /locus_tag="SCK08_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEG1200455.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14293..14640
                     /gene="rbfA"
                     /locus_tag="SCK08_000542"
     CDS             14293..14640
                     /gene="rbfA"
                     /locus_tag="SCK08_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEG1200456.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14797..14970)
                     /locus_tag="SCK08_000543"
     CDS             complement(14797..14970)
                     /locus_tag="SCK08_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1200457.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15418..17780
                     /locus_tag="SCK08_000544"
                     /pseudo
     CDS             15418..17780
                     /locus_tag="SCK08_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18148..19071
                     /gene="truB"
                     /locus_tag="SCK08_000545"
     CDS             18148..19071
                     /gene="truB"
                     /locus_tag="SCK08_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEG1200458.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            19075..20016
                     /gene="ribF"
                     /locus_tag="SCK08_000546"
     CDS             19075..20016
                     /gene="ribF"
                     /locus_tag="SCK08_000546"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEG1200459.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20368..21153
                     /locus_tag="SCK08_000547"
     CDS             20368..21153
                     /locus_tag="SCK08_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1200460.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21166..22017
                     /locus_tag="SCK08_000548"
     CDS             21166..22017
                     /locus_tag="SCK08_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEG1200461.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22141..22593)
                     /locus_tag="SCK08_000549"
     CDS             complement(22141..22593)
                     /locus_tag="SCK08_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1200462.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(23058..23426)
                     /locus_tag="SCK08_000550"
     CDS             complement(23058..23426)
                     /locus_tag="SCK08_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEG1200463.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23576..24058
                     /locus_tag="SCK08_000551"
     CDS             23576..24058
                     /locus_tag="SCK08_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEG1200464.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            24095..25105
                     /locus_tag="SCK08_000552"
     CDS             24095..25105
                     /locus_tag="SCK08_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEG1200465.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25220..25933)
                     /locus_tag="SCK08_000553"
     CDS             complement(25220..25933)
                     /locus_tag="SCK08_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200466.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(26123..26713)
                     /locus_tag="SCK08_000554"
     CDS             complement(26123..26713)
                     /locus_tag="SCK08_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEG1200467.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27197..27424
                     /locus_tag="SCK08_000555"
     CDS             27197..27424
                     /locus_tag="SCK08_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEG1200468.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27440..28606
                     /locus_tag="SCK08_000556"
     CDS             27440..28606
                     /locus_tag="SCK08_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEG1200469.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28840..29883
                     /gene="hrcA"
                     /locus_tag="SCK08_000557"
     CDS             28840..29883
                     /gene="hrcA"
                     /locus_tag="SCK08_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEG1200470.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29977..30540
                     /gene="grpE"
                     /locus_tag="SCK08_000558"
     CDS             29977..30540
                     /gene="grpE"
                     /locus_tag="SCK08_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEG1200471.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30591..32420
                     /gene="dnaK"
                     /locus_tag="SCK08_000559"
     CDS             30591..32420
                     /gene="dnaK"
                     /locus_tag="SCK08_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEG1200472.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32571..33737
                     /gene="dnaJ"
                     /locus_tag="SCK08_000560"
     CDS             32571..33737
                     /gene="dnaJ"
                     /locus_tag="SCK08_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEG1200473.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33819..34484)
                     /locus_tag="SCK08_000561"
     CDS             complement(33819..34484)
                     /locus_tag="SCK08_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1200474.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34460..34633
                     /locus_tag="SCK08_000562"
     CDS             34460..34633
                     /locus_tag="SCK08_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200475.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34678..35415)
                     /locus_tag="SCK08_000563"
     CDS             complement(34678..35415)
                     /locus_tag="SCK08_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1200476.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(35417..36919)
                     /locus_tag="SCK08_000564"
     CDS             complement(35417..36919)
                     /locus_tag="SCK08_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEG1200477.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37178..39172
                     /gene="uvrB"
                     /locus_tag="SCK08_000565"
     CDS             37178..39172
                     /gene="uvrB"
                     /locus_tag="SCK08_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEG1200478.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            39182..41998
                     /gene="uvrA"
                     /locus_tag="SCK08_000566"
     CDS             39182..41998
                     /gene="uvrA"
                     /locus_tag="SCK08_000566"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEG1200479.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            42261..43526
                     /locus_tag="SCK08_000567"
     CDS             42261..43526
                     /locus_tag="SCK08_000567"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEG1200480.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            43530..44261
                     /locus_tag="SCK08_000568"
     CDS             43530..44261
                     /locus_tag="SCK08_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEG1200481.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            44396..45280
                     /gene="rapZ"
                     /locus_tag="SCK08_000569"
     CDS             44396..45280
                     /gene="rapZ"
                     /locus_tag="SCK08_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEG1200482.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            45277..46275
                     /locus_tag="SCK08_000570"
     CDS             45277..46275
                     /locus_tag="SCK08_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEG1200483.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            46309..47244
                     /gene="whiA"
                     /locus_tag="SCK08_000571"
     CDS             46309..47244
                     /gene="whiA"
                     /locus_tag="SCK08_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEG1200484.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            47787..48437
                     /locus_tag="SCK08_000572"
     CDS             47787..48437
                     /locus_tag="SCK08_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200485.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            48624..>49184
                     /locus_tag="SCK08_000573"
     CDS             48624..>49184
                     /locus_tag="SCK08_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEG1200486.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        16466 a         8421 c        10575 g        13722 t
ORIGIN      
        1 ttaaaaaaag aaaaaattat tcagttttta aataaaaaaa caatgatttc ataggaaaaa
       61 cagttagata ttgctataat acttaagata gtgggaatct tttaagtata ggaggaaaaa
      121 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
      181 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      241 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      301 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      361 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      421 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      481 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      541 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagc agagcagaga
      601 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      661 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      721 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      781 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      841 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      901 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtggtca cagcagccgt
      961 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
     1021 agcaatctta gcagtaatag gagtttacga gctgtttcgg atgaaaggat tgacactctt
     1081 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1141 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1201 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1261 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1321 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1381 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1441 aacgattgaa ggtgcgctcg gtggtattgg tagtgctgtt gtagtcgcat ttctttattt
     1501 attgctatac ccagcaaaag aactatttaa tcatggtatg tttgtcatgc ttctgatgac
     1561 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1621 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1681 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1741 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1801 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1861 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1921 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1981 gaatcctcgt tcgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     2041 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     2101 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2161 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2221 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2281 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2341 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2401 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcctgttat
     2461 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2521 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2581 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2641 cggataaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2701 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2761 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2821 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2881 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2941 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     3001 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     3061 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3121 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3181 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3241 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3301 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3361 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3421 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3481 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3541 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3601 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3661 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcgta ttctttccat
     3721 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta caccgaagta
     3781 ttcaagcgtt gtggcttaga atttcgctcg attatcggag acggtggcgc aatgggcggg
     3841 aaagactcaa aagaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3901 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3961 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     4021 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     4081 gcggatgaac aacctgtcct tgttttggta cgcggagacc atgaagtcaa tgacgtaaaa
     4141 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4201 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4261 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4321 ttccactata aaaatgtgaa tccagaacgc gatttcactg tactttctta tgaagatctt
     4381 cgttttgtac aagaaggaga tccttcacca gacaacaatg gtgttcttgc ctttactcga
     4441 ggaatcgaaa tcggacatat cttcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4501 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4561 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaactgg
     4621 ccaaaaggaa ttgcgccatt tgatttgcat atcgtccaaa tgaatctaaa agacgaatac
     4681 caaacaaacc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4741 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4801 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4861 aaaactggcg aaatggttga agtacgtaaa gaagaattgg tgaatacttt accaatcctc
     4921 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4981 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     5041 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     5101 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5161 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5221 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5281 ggaatttacg ctgcattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5341 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5401 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5461 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5521 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5581 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5641 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5701 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5761 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5821 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5881 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5941 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     6001 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     6061 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6121 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6181 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6241 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6301 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6361 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6421 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6481 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6541 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6601 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6661 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6721 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6781 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6841 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6901 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6961 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     7021 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     7081 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7141 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7201 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7261 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7321 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7381 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7441 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7501 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7561 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7621 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7681 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7741 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7801 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7861 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7921 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7981 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     8041 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     8101 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8161 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8221 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8281 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8341 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8401 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8461 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8521 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8581 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8641 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8701 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8761 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8821 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8881 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8941 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     9001 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     9061 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9121 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9181 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9241 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9301 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9361 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9421 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9481 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9541 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9601 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9661 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9721 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9781 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9841 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9901 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9961 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
    10021 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
    10081 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10141 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10201 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10261 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10321 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10381 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10441 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10501 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10561 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10621 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10681 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10741 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10801 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10861 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10921 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10981 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    11041 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    11101 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11161 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11221 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11281 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11341 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11401 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11461 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11521 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11581 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11641 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11701 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11761 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11821 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11881 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11941 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    12001 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    12061 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12121 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12181 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12241 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12301 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12361 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12421 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12481 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12541 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12601 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12661 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12721 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12781 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12841 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12901 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12961 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    13021 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    13081 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13141 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13201 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13261 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13321 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13381 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13441 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13501 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13561 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13621 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13681 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13741 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13801 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13861 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13921 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13981 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    14041 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    14101 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14161 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14221 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14281 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14341 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14401 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14461 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14521 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14581 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14641 tcaaaaactc ccgcaaatcc tgtgaaatca ggggttgcgg gatttttttg ttttcattaa
    14701 gacgtattac ctaagagttc catgacttta gtcttgcggc gtgtcagcta accaaaaaag
    14761 agaaaaagaa aagtaaaaaa ttcgactttc ttttttctag tagtatataa atttttgcat
    14821 atagataaag atcaagatac cgataacgga agctagattg taatctattt tagtgacagt
    14881 caggtatagc caagtaacta gaagatcagt ggaacctgca ccataaaagt ttgagtttgt
    14941 cggtccgttg acagttaaaa gaaaaatcac gttgaagtta ttgatatttc cgattacgcc
    15001 gcttcgattt ggtcacgatc caaattttgg ataatccctg tagaaattag catagtagct
    15061 gggataccaa ttcacatatt gacaaagata acagagactt ttacataaaa cggatcaatc
    15121 aggaaaggaa ttgctcgatc cagccccagt tttccagcaa tgtgttaact ggcccagcgt
    15181 cattcagcaa attatgcatg atgaggagcg aaatgaactg cggaacagcc attgtgataa
    15241 cgacaagttt tttggatcaa tgtaaaaaac gtcagcgaaa ggaaaaattt cgtggttttt
    15301 tatacctgtt catttggcta gatataaaat ccaaacaaat gaacagaagt gcttattagg
    15361 tttcaattgg gagtatctgc gctagaataa gagtaataat gaaaaggagt gttatgtatg
    15421 gattattcta gtaaagaata ctttgataaa atgacggctt ggtggcgtgc tgctaattat
    15481 ctttctgttg ggcaacttta tcttaaagat aaccccttac tgagacgtac actgaaaccc
    15541 gaagatgtca aaaaacatcc aatcggtcat tggggaacga ttcctggcca aaattttatt
    15601 tatgtccatt tgaaccgagt catcaataaa tatgatttga acatgtttta tattgaaggg
    15661 ccaggacacg gcggacaagt catggtatcg aatgcttatt tagatggaag ttacacagag
    15721 atctatccag aagtaacgga agatgagaca ggaatgcaaa aattattcaa acgattttca
    15781 tttcctggag gcattgcttc tcatgcagca ccagaaacac caggttctat ccatgaaggc
    15841 ggagagcttg gatactctct ttcacatgca gtcggagctg tattagacaa tcctgaagta
    15901 atctctgctg tcgtgattgg tgatggtgaa gcagaaaccg gtcctttagc tggaagctgg
    15961 ttctcaaatg tctttatcaa tccagtgata gatggagctg ttttaccgat ccttcattta
    16021 aatggtgcaa aaatagccaa cccaacgatc ttggcacgta aatcagatgg agaattagca
    16081 aattacttta acggacttgg ctgggaacct ttctttattg aaggaaatga ttcagaaaaa
    16141 ttaaatcctg ttatggctga aaaaatggat caagcaatcg agaaaattaa atccatccaa
    16201 aaagaagctc gcctgaagac agcaacagat gtcgtgatgc caaaatggcc agtattgatc
    16261 gtacgcacac caaaaggatg gaccggtcca aaagaatggg acggggaacc aattgaagga
    16321 accttccgag cacaccaagt accgattcct gtagatcaag aacatatgga tcatgcagat
    16381 gctttactcc gttggctgaa atcctatgaa ccagaaaaat tatttgatgc acaaggacgt
    16441 attttagaag aaatcagaga aatcgcgcct actggtgacc aaagaatggc aaaaaatcct
    16501 atcaccaatg gtggaatcga tccaagaccg cttattatgc cagattggaa aaaatatact
    16561 ttgcaatttg aaaaaccagg ctccgttaaa gcagaagata tgaccgaact tggtaaattc
    16621 gttcgcgaga tcattgagaa aaatccagaa aatttccgta tatttggacc agatgaaaca
    16681 aaatcaaacc gattgaatca agttttcaaa acgacaaatc gccaatggat ggaaaaaatc
    16741 gaaccagaaa atgatgaatg gctttcgcca tctggtcgcg tcatcgacag tcaattatcg
    16801 gaacaccaag atgaagggtt cttagaaggg tacgttctga ctggacggca tggttttttg
    16861 caagttacga atcattcttg cgagtggttg actctatgtt gactcagcac ttcaaatgga
    16921 tgcggaaatc acatgacctt tcttggcgaa atgattatcc ttcgctaaat ctcattgcaa
    16981 gctcaacagt cttccagcaa gatcacaatg gatatagcca ccaagatcca ggaatattga
    17041 cacatttggc agaaaagaag gcggagttca tccgtgaata tctgcctgcg gatgcaaata
    17101 cattgctcgc agtcatggat aaagcattcc gctcgtcaga aaaaatcaat ttgatcatct
    17161 cttccaaaca tccgcgtgct caattttact cagcagaaga agcagccgtc ttagtgaatg
    17221 aaggcttgaa aatcattgat tgggcttcga cagcaaaaga ggaggaacct gaattggtca
    17281 ttgctgctgc aggaacggaa tctaacttag aagcattagc agctgtaact ttgctgctgg
    17341 aagaattccc aaaactaaaa attcggttca tcaatgtcgt agatttattg aaattacgtc
    17401 atccaagtca agatccgcgt gggttgagcg atgaagaatt tgatcaatat tttacaaaag
    17461 acaaaccgat cttatttgct ttccacggtt atgaaacatt ggtccgcacg attttctttg
    17521 atcgtcataa ccatcattta atgattcatg gctacaaaga aaatggagat atcacgacac
    17581 catttgacat gcgtgtagta aatgaacttg atcgttatca tttggcaaaa gacgcagctt
    17641 taaaaatcaa aggaagtcaa gcagaagatt ttgcgaaaaa aatggatcag aaattacaag
    17701 aacatcaaaa ctatattcgt gaaaatggta ttgatctgcc agaagtattg gattggaaat
    17761 ggaaaaatct tgaccaataa aatgataaaa tcgatcccgc aaaatagaat tttttacatt
    17821 gattaagaaa ctgaaaaaat aaataaaaaa aacggaaaaa agactggaag aatggccagt
    17881 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
    17941 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaagcataa
    18001 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
    18061 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
    18121 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat tacctttatg gaaagaacgc
    18181 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
    18241 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
    18301 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
    18361 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
    18421 gatatgtctt tatctgaaaa aaaaatagat gaagtgatgg cttcatttat tggtgagatc
    18481 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
    18541 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
    18601 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
    18661 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
    18721 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaggat
    18781 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
    18841 ctgccaatag aaaaaggagt ggaacggttc aaacgagtag atattactga tgaagtatgg
    18901 cagaaagtaa aaaacggtat gagacttgat tatactgtat ttgcgctccc agaaatgccg
    18961 tcagaagaaa ttgctttgtt ttataaagga aaagttgtca gtatttatca gccaaacccg
    19021 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
    19081 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
    19141 gtgctgggat ttttcgacgg cgtgcatatc ggacaccaga aagtcataaa gacaggtatg
    19201 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatct
    19261 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
    19321 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
    19381 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
    19441 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
    19501 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
    19561 ggtgaaaaaa tcagctctac ccgtattcgt ggattactag acagcggaca agtaaaagaa
    19621 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
    19681 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac ggttcatttg
    19741 cctaaagaag gtgtttatgt gtcggaaata aaaattgggg aaacatggta tccagccatg
    19801 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agagttgtat
    19861 attttggact ttaatcaaga tatctatggt gaacatgtcg cggtacgctg gaatcaattt
    19921 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaggaggat
    19981 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
    20041 ttatgtataa ctagcaatta caataaaatg ggtgataaat aagcccagga aaggggctgt
    20101 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
    20161 cggaaataag ccgaaatttc ttgaagctga ccacttctgt cacaacctct ttctttatag
    20221 aaataaaagg acactagcaa ttcatgaaaa cgattgctag agcgtatcat ttgctttaga
    20281 ctatatatct tggtgtacac tcactttaag gcggaaataa aagttatccg taataacagg
    20341 tttaggtatg aaaaaggagt gggtactatg tacaaggaat tagaaaacaa agtcgcagta
    20401 gtaactggtg gctcaaaggg gattggaaca gcgattgctc aacgattcgg gaaagaaaaa
    20461 atgaatgtag ttgtcaacta taactcggat aaagaaggag cagaaaaagc agtcaaagca
    20521 atcgaagagg caggcggaaa agcagtctct gtacaagcag atgtcagttc tgaagaagga
    20581 atacagcgtt tacttgatac ggctttagaa aattttggga cactggacgt ttgggttaat
    20641 aatgctggga tggaaaatca gcatccaacc catgaactta gccttgaaga ttgggaaaga
    20701 gtgttaaaag tcaatttgac tggcgttttt atcggaacga aagcggcttt aaattacttt
    20761 acagagaata ataaaaaagg aaatatcatc aatatgtctt ctgttcatga acagatccca
    20821 tggccaacgt ttgctcatta cgctgcttca aaaggcgggg taaaattatt taccgaaacg
    20881 gtagcaatgg agtatgcacc gaagggaatc agaatcaata gtataggccc tggtgctatc
    20941 cgtacaccta ttaatgcggc aaaatttgat gatcctgaac aaaaagaact cttggaaagt
    21001 atgatcccac taggacgcgt aggtgaacca aaagaaatag cggcgacagc tgcatggttg
    21061 gcttcagacg aatccagtta tgttacaggt attacactat ttgcagatgg tgggatgact
    21121 ttatatcctt ctttccaagg tggaaaagga taagggtgaa taaaaatggg tttattgatt
    21181 gcattgatcc cggcagttgg ctgggggatc cagccactga ttttgaaaaa gattggtggc
    21241 agaccaacaa atgagatatt aggaactggt attggagcgg tgcttgtcgg cttactcgtt
    21301 caattattcg cttctccagc tggtatttca gtgactacat ttttgattag tttactttct
    21361 ggtgcatttt gggttattgg acaaacagga cagtatacat ctttcaactt gattggtgtt
    21421 tctaaaacga tgccaatttc gactgcatta cagttagtcg gaacctcgtt gattggtgtc
    21481 ttcgcttttg gagaatggtc tggaagtatg gggaaaatca ttggagcagc agcaatcatt
    21541 cttcttgtat ttggttctgc tttaacggca atcagtgatg atcctggaaa aaaaggcggt
    21601 atcacaaaag gaattagtct attagcatca acaagtattg gttattgggt atacagtgca
    21661 ttgcctaaac tagttgatgc aagcggactt tccattttct ttccgcaaat gatcggtgtc
    21721 tttttagggg cagtgattta tgtgattttc cgtaaaccaa ccgcattttc tgaagacaag
    21781 agttggaaag caacgatcgt tggagccatc tttagtgtgt cagcattggc ttatattttt
    21841 tcagcaaatg aaaatggggt tgctactgca tatatcatta cacagctaaa cgtagttatc
    21901 tctacattag gcggtatgta catcctgcat gaaaataaga caccaaaaga aaaaaaatta
    21961 acgattatcg gattattgct gatcgtagta ggaagcatgg tcacggtctt tatttgagga
    22021 taaagatggt aaaatttcat ttattaaaaa aatatttgtt tgaaccaaac agtaagtact
    22081 tataaaaaaa tagcgctaga atttccaaca tctctggaat ttctagcgct ttttaattgt
    22141 ttaagataca agggaaatat ctgtctggat caataatttt tcattgatcc attgactaaa
    22201 gtttacgaca taattgaagt taccaccgat tagtatctcc gcatgattgt ctttataaag
    22261 atcgtattgt aaaagacggt cttttttatt tgcgtaaaca tcgttatggt actggactaa
    22321 ttgttctcgg aattgattga ttgctgtaaa atcacgatat tgaaggttga aacgcatcgt
    22381 gattggtgca tttagcatga atcgctgcat ttcttttgct gcttcctctg cttgatctcc
    22441 gtataggaac tcaaaaagat tacgttcaga aaatttccat tgcccgccaa cctttgttcc
    22501 ttttagtttt ccttcatgaa gataatttct gatagaacgt gtagtaacac ctaattgttc
    22561 ggctaattca tttactgagt aaaaatcttt caatgttcac cctcctatgt gaatggttct
    22621 actgacttag ttgatatttg cttaatcacg aaatgtagaa agaacgattt taattaaggt
    22681 cattgtaaaa atcaagtcag aaaattctaa cttttatttc tctaaagaaa ttcgttctct
    22741 cctaaagtga tattatattt aaatatacct gaaaataatg ggcagtatta tgaaagatca
    22801 ctgatatttt ctcgtatttt ttccttattc aaaagttttg caggaaaatt gctgaaaaaa
    22861 taattttact atgtagaaaa ttcaaagcca ttcaatcagt gatttttttc agcacaagaa
    22921 agactcctta taaaaatagc agatactttg tcaattaagt gtttaaccag ggatgataaa
    22981 aaggatagga ttgaacaaaa cctgtataag gtgatgaata gtttcctcgc cagtaaaagg
    23041 tttattcgta taaagcttta tcgccaaaat ttatcatgac cttttttatc ttcaatatta
    23101 aatgaaatca attgttcaat caattcccag ttgattacac ttttccaagg aactttgatg
    23161 acgttatccg tatatacgta gcctgcttct tggatatttt tttcaagggc acgaatcgct
    23221 gctttttccg gtgcgataga aaaatgattt ttagcaaaac taaatgcaat aataaaggta
    23281 ccatggtcag taaacatagg ttggttccat tttacagttg tgtctagctg gggatacgtt
    23341 tctgttatct tagcaaaaag gtctttgatc ttttgttgat gttctttttc tttgatttga
    23401 ttgatgatcg gctgaaattt ttccattcta aaacgcctcc attaagtggt ttgatttatt
    23461 ttaccagaaa aacaatagtt atgtataaaa aacgaacaaa acacaagtaa tgttgtaacc
    23521 gctatattta tatgataaga tgtctcaaat aataaaatag aaaagagaca taaaaatgat
    23581 tgacttggca aagaaattct tacacgaaaa tatatatttt gagttgtatc atcatcgagc
    23641 catttataca aatgaagatg ctttggtaat aaagaaagaa caaggattta caggtacaga
    23701 gaccaaaagc ttattcttaa aagataaaaa agataataac tacgtatatc tgacttttac
    23761 tacgaaacaa acggatttca aagcattagg gaagatcgtc ggtaaaaggc tttctattgt
    23821 ttcatcagaa cgcatggaag aacaaacggg acaaaagcag ggcgctgttt ctccgtttgg
    23881 ttatgaaaaa cagattccga ttattattga tgaagagctc ttatcaaatg aaaaacttgt
    23941 ttttgcacca ggtagaccag atcaaacgat ggtcatccaa acggttgact taaacaaaat
    24001 catccagcta cttgagatgc cgatattttt gatgccggca aacgaaaaaa acaattaacc
    24061 aagtaaaata gtgcatactt ggaggtgaag caacatgata agttatccat tattagaaag
    24121 aaatgcaacg attggggtta ctgcaccatc ctcaggcgta tcagaggaac accattcgtt
    24181 actcgaatca gctttaaaga gaatggaaga aaaatctttc caagtagtta ctggaaagac
    24241 gctttggacg caagataagg aaaaatcagg tccagcaaga aaacgagcag atgaattcaa
    24301 tcaaatgatg caagatgaaa atatccaaat gatctttccg acttggggag gagaattatt
    24361 gattgagatt ctcgattttg tcgattttga tgctctacaa ccaaaatgga tagtaggtta
    24421 ttcagatatt agtacgttgc tacttgctgt cacattaaaa acagggatag ccactgcaca
    24481 tggaactaac cttattgatt taagaggaga gtacttggat gaaacgacag caatgtggga
    24541 aagtgtactg aaaacaagag aaggagaatc gattctacag caatcttctg aaaagtatca
    24601 aaaagtgtgg ccccatgagc attcatcatc ttctgttttc catctcactg aaccaacaaa
    24661 ttggcaatca gtcagcggag atgcagtaag gatgaaggga cgattattag gtggttgtat
    24721 tgatacgatc aggcatttaa tcggtactcc ttatggagat gtccaatctt ttaaaagaaa
    24781 gtataatgat ggaaagccga tcttgtggtt tctagaaaac tgtgagctat cgtcagttga
    24841 tttaagaaga tcacttgttc agatgaaact ggcaggctgg tttgatggca gctcggggat
    24901 catgttcggt agaagtgcgg cagaggattt gttagattat aaaatgaagg atgtatacga
    24961 agatctgtca aaggaattaa aactaccgat cttgtatgat attgattgtg gtcatgtacc
    25021 gccacaactg acattgatta atggtgccta tgcagaagta gcggtagaag atagaaaagc
    25081 gactgttttg caaacgttta tttaaaaaat tttttcctgc cttggagtct atcaaagacc
    25141 aataaatcta aatagaaaag aaagtgaaag aagctgacaa aatgctgtca gcttcttttt
    25201 gtataacata ttagaaagtc taattatcta taactgtcac atcgattaac tgtttcacgt
    25261 cattgggatt tcctgtagga atagctacta cagatgcatt atcaatgatt tcttgagttg
    25321 tttctctaat gaacgctgga taagatgttt gatctgcagg catttgccaa tgtgaaggaa
    25381 ctggataact agtgattgtc ccgtcatggt tacttgaata cactaaaact ccctggtaac
    25441 tgtatttccc agtgagcata accacatctt tcggatagac tgcactccct gcatcataag
    25501 gggcgatagg tgttcctgca ggataatgaa taacatgtaa ttcgaaatca cccgatgtat
    25561 ctctaaaagt attacctcta atagctagcc aaacacgtgc gtactcaatt tgtagatcag
    25621 aatatcctgc taacggatct attgactgag aagactgtga ggaggttgtg tctattgttg
    25681 aagaagaaat atttgtctca actgtactac tagatgtaga agaagtattg ggctctgaga
    25741 ctttactgct tgttgaagat gtattcgttt cagtagaagc tgatttctga gtacttgatt
    25801 tttcagctga actggtttgt ctgattgaac ttttgtctgt ggtcgcagat ggatcactat
    25861 tttctttact aggagacgta catcctacaa ggaataaaga gaccaacaat aaaataaaac
    25921 ctaacttttt catataaaat acctcattat ttttttaata ccagtatagc ataatcctga
    25981 atttttgaat ccgtaaaacc ttgtctatga atataaatag agtgtatctt ctttgataaa
    26041 tgtcagaaaa agtgtgacaa ataatcatca gtaaaattac ttgtcacact attgataagg
    26101 agtatttaat tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat
    26161 agaatccaag tttgtataga cgaatcgttt tttctcttat ttcttctgtt tcaaaatgga
    26221 ctgcttgaag aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat
    26281 tacgatcttt cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt
    26341 ctaaccaagg aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag
    26401 ctaaataatc aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat
    26461 cgattatttg atataaatga tgatttttta tgtcccatga attgccaccg atcataataa
    26521 aaaggtcaac tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc
    26581 caatggaagt gacctctgat tggatagaag cagttttgtt ttgccatttc tcattttggt
    26641 ttagtgtgct agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa
    26701 tcgcagtttt cattttcaag ctctccttta ctatctataa caaggacaac agttttaacc
    26761 tacagacatt cattaaaatt gggtagcgac agcgaaaaca acaattctta agttttagac
    26821 agagtataca gatagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat
    26881 ttttgtttac cagcattata acattttgca tacatggatg acaaaagaac caattcccac
    26941 ttgcttgagt acaatattaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa
    27001 taaatcatat cgggcactca gacatgaaaa caaccctaga gatatataac taggtaacca
    27061 gtttaatgaa agaaaaaatc atccaagaag tcgattcttg gattttttag tggatttgtt
    27121 cgccaaatac gtttagtatg cgtatatgag aggttgtaag caaagcgttg tggtctgagc
    27181 acttaaggag gaatctgtga aaatagcttt tcatattttt gcagattttg acttaatgcc
    27241 gaaggtcagc tttgcgatta ccgtttatcg agaggctgta aaagcaccgt gtagctccga
    27301 tgacaataag caagcatttt tagagctagg tttccttgat tctaaatatg cggcttattt
    27361 caatgagttg gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg acttttcgac
    27421 gtaaggagaa agaatttcaa tgatagaaac aataaatccc gcagaaaaca aggtttctgc
    27481 gtacattcat atcccatttt gcgagcatat ctgctattac tgtgatttta ataaagtttt
    27541 tttagaaggg cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa tgaaaataat
    27601 gctagaaagt catccgacag cgaatttaga aacactttac gtagggggcg ggacaccaac
    27661 ttctttatct gctaaacaac ttgatcgact gttatcaggt gctagagaga tcctgccttt
    27721 tcaaaatggt aatgagttta ctgtagaagc gaatccaggt gatttgaccc gtgaaaaact
    27781 tcaagtaatg aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga catttgataa
    27841 tcgcttattg aaaaaaattg gacgtaaaca caccgcagaa gatgtgtatc aaactatgca
    27901 atttttagaa gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg cattaccggg
    27961 acaaacacta gaaggatatc tggatacttt ggaaaaagct ttggctttgg atctgcctca
    28021 ctattctttg tactcattga tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca
    28081 aggaagactc gaactgcctg atcaggaaac ggaaaccaga atgtttgaag agacgattga
    28141 agcaatggaa aagcatggac gtcaccaata tgaaataagt aactttgggc tctccggaca
    28201 tgaatctcaa cataacctca tgtactggaa caatgaccac tattttgggt ttggagctgg
    28261 tgctagcggt tatcttggag atattcgtta ccgcaacaaa ggaccaatcc aacactattt
    28321 acgcccatta cgagcaaatg aacgcccggt attggaagaa gaaaaattga cacgaaaaaa
    28381 tcaaatagaa gaagaaatgt tcttaggact aagaaaaaaa acagggatta ctaaaaaaca
    28441 tttctatgat cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc taagggaatt
    28501 agagcaagaa gggttacttg ttaatgaaga agaccgtgtt tatttgacac caagagggac
    28561 ctttttgggg aacaatgtgt ttgagcgctt tttgtttgaa aaatagttta aagaatattt
    28621 gttttttatc tgaatttaaa taaaaaaagt tcaaaaatac aataaaggac tattgaaacg
    28681 ttgatataac tggatttaca tcaattattt agaattagca ctcttgtgtt tagagtgcta
    28741 aaaaatggag tctttctctt gacaggagaa gggcttcttg ctatatttat aagtggatta
    28801 gcacttagaa tatatgagtg ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaac
    28861 gacattttac gtctgatcat ccaacactat acgagtagtg gcgtgcccgt tggttccaaa
    28921 acattgatgt ctgagggagt caaggccagc tcagcaacga ttcgcaatga tatgaaagca
    28981 ttagaagatg aaggattgct gttgaagact cactcttctt ctgggcggat tccatctgct
    29041 ttaggctatc gttattacgt ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat
    29101 ttgctgctga ttcgtcagtc tctaggcaga gaatttcatg aaatcaatga gattattaag
    29161 caatctgctg agatattatc tgagctgaca agctatacga ctttttcatt gggacctgaa
    29221 ataaaagacc gccgactgac tggctttcgt atcgtaccct tgaataatcg acagattatt
    29281 gctatcgtcg taacagatat ggggaatgta gaaagccaag tgttcactct tcccgaatat
    29341 atgggaagtc aggaactaga aaaaatggta cagattgtaa acgaccgtct agtcggagat
    29401 cctctgatga cggtgtacca taaacttcgt acggagattc cgatgattct tcataaatat
    29461 ttccaaacga cagaagggat gtctcagtta tttgacaccg ttcttggtca tgtttttgaa
    29521 gataaggttt atgtcagtgg acaaatgaat ctcttggatt atgagccccg tcaagatctt
    29581 gatcagttca aatcgatgtt ttcttttatg aaagactcag acggacttac acaaatgatc
    29641 actccgatgg atagcaatat tcatatcaag attggtgctg aattaggcaa tgatttattg
    29701 cagaatatga gtatgattca agcaagttac gagatagatg gtcatggaag aggtacaata
    29761 gccttgttag ggcctactag tatgccttat tctcggatgt tcggtctagt cgatgtttat
    29821 cgaagagaac tagcagctaa acttgctgat tactaccgtt cgcttgactt gtcaaactcg
    29881 taatgtgaat aacgaagcta tttgcagctg atcgttctct ctttcttagt aagagcaatc
    29941 aaacagctga ctgaataatg aaaggaagac tgtatcgtga aagaaaatca agaagaacta
    30001 gataaagaaa tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat
    30061 tcgggtattt cagaagctga agcggaagaa ttcgaaacag cgaaattaaa agctgagtta
    30121 gaagaaatgg aagataaata cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga
    30181 ggaaaaaatg agcgtgagca attacaaaaa taccgttcac aagatttagc caaaaaatta
    30241 cttccatcaa ttgacaacct ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg
    30301 gcaggtttga aaaaaggcgt ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa
    30361 gaaggaattg aaaaaatccc tgctaaagga gaagcctttg atcctaatct tcatcaagca
    30421 gtacaaactg ttccagcgac tgaagataca ccagccgata cgattgtaga agtactacaa
    30481 gaaggctaca aattacatga tcgcgtgtta agaccaacca tggtcattgt tgcacaataa
    30541 ttgtgcggat ataaaactta cactttcaat taaagaagga gacgttaatt atgagtaaaa
    30601 ttatcggtat tgacttaggt acgactaact ctgcagtagc agtattggaa ggaaacgaag
    30661 caaaaatcat cgctaaccca gaaggaaacc gtacaacacc ttctgttgta tcattcaaaa
    30721 atggagaaat ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga
    30781 tttcatcaat caaacgccat atcggtgaag caggttacaa agtagatgta gaaggtaaat
    30841 catatacacc acaagaaatc tcagcaatga tccttcaata tatcaaagga ttcgcagaag
    30901 actatttagg tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac tttaatgatg
    30961 cacaacgtca agcaacaaaa gatgctggta agatcgctgg tctagaagta gaacgtatcg
    31021 tcaatgaacc aactgcagct gctcttgcct atggtttaga taaaacagat cgcgacgaaa
    31081 aaatcttagt attcgacctt ggtggcggta cttttgacgt atctatcctt gaactaggag
    31141 acggtgtctt tgatgtattg tcaacagctg gcgacaacca tctaggtggg gacgactttg
    31201 ataacaaaat catcgactac atggtagcag aattcaaaaa agaaaacagc attgacttgt
    31261 ctcaagacaa aatggcttta caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt
    31321 tatctggcgt atcaagcaca caaatcagct tgccatttat cacagcagga gaatctggac
    31381 cattgcattt ggaaatgaca ttgacacgtg caaaatttga tgaattaaca gctgacttag
    31441 tagaacgtac aaaagtacca gtacgtcaag cgctaaaaga tgcaggttta tcacaatcag
    31501 aaatcgacga agttatttta gtcggtggat ctactcgtat ccctgcagtt gtagaagcag
    31561 tacgtaaaga aacaggaaaa gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta
    31621 tgggtgctgc tatccaaggt ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg
    31681 acgttacacc attgtcatta ggtatcgaaa caatgggtgg agtgttcact aaattgattg
    31741 accgcaatac aacgattcca acaagtaaat cacaagtatt ctcaactgcg gcagataatc
    31801 aaccagcagt agacatccat gtattacaag gtgaacgccc aatggctgct gacaacaaaa
    31861 ctctaggaag attccaattg acagatattc cagcagctcc tcgtggtatt ccacaaatcg
    31921 aagtaacatt cgatattgat aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta
    31981 cacaaaaaga acaaaaaatc acgatcaaat cttcttcagg tctaacagac gaagaaatcg
    32041 aacgtatggt aaaagatgca gaagcaaatg cagaagcaga taaagctcgt aaagaggaag
    32101 ttgacctacg taatgatgta gatgcattgc tattctctgt ggacaaaaca ttgaaagaat
    32161 tagaaggcaa agtggacgaa gaagaagtga aaaaagcaga aacagctcgc gatgaattga
    32221 aagccgctgt tgaagcaaac aacatcgaag aaatgaaaac taaacgcgat gcattgaacg
    32281 aaatcgttca aaacttgact gttaaactat acgaacaagc ggcacaacaa caagctcaag
    32341 aaaacccaga agcagctcaa ggcggagcag atgatgtcgt tgatgctgat tttgaagaag
    32401 tcgatggcga cgataaataa tcagttcact gaatttcgag agaaggccaa agttgctact
    32461 ttggcttttt cttttgaaaa atagtttgaa gtatcggttt ttgtttcaaa caaaatatga
    32521 tatgataaca agcgacgtga aaatgatgag aaccaatagg aggaaagcct atggcaacaa
    32581 aacgtgatta ttatgaagtc ttagggctct caaaaggagc atcagaagat gaaataaaga
    32641 aagcttatcg taagctttcg aaaaaatacc atcctgacat caataaagaa cctgatgcgg
    32701 aagaaaaatt caaagaagta tcagaagcat atgagatttt aagtgacccg caaaaacgcg
    32761 ctgcatatga ccaatacggg catgcaggca cagatccaaa ttacggtgct ggtggagctg
    32821 gtggcggatt tggtggattc ggcggcttct ctggtggcgg cttcggtggt tttgaagata
    32881 tcttcgactc tttctttgga ggaggcggtc ggtcagtcga tccaaatgcg cctcgtcaag
    32941 gtgctgattt gcaatatacg gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa
    33001 aagaaatcaa gtataatcgg gaagaaatct gtcatacttg tggtgggaac ggtgcaaaac
    33061 caggcacaca accaactact tgtcataaat gtcatggtgc aggtacgatt aatgtagaac
    33121 gccaaacacc acttggacga gtgatgagcc gtcaaacttg tgatgtttgt catgggacag
    33181 ggaaagaaat caaagaacct tgtccaacct gtcatggctc aggacatgag aagaaagcac
    33241 attcagttaa agtgaatgta ccagctggtg tagaagaagg acaacaaatg cgtttagctg
    33301 gtcaaggaga agctggagaa aacggcggac catttggtga tttgtatgtg gtcttccgtg
    33361 tagaagaaag tgatatcttt gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa
    33421 gttttgtaca agcagctttg ggggatgaag tccaagtacc aactgtccat ggagatgtga
    33481 aattgaagat ccctgctgga acacagacag gtacgaactt ccgtttacgt ggaaaaggcg
    33541 ctccgaagtt acgtggtggt gcaacaggcg accaacatgt caaagtaaaa ctgatcacac
    33601 cgaaaaatct aaatgaacaa caaagagaag cattacgtgc attcgctgaa gctggcggca
    33661 tcaaagttga agaacagcaa gaagacggtt tcttcgataa gtttaaagat gcttttggcg
    33721 ggaaaaagcg gaagtagttt ttctagtaaa aacgctaagt cattaataat taaaaaccag
    33781 acgaacaatc atatgttcgc ctggtttttt tgtagttgtc atgtttgtac caacaaaaaa
    33841 gccagcttta gaatatgata atcgtcatag tttctagggg tataaccagt gagagaatgg
    33901 aacttgttca atcgatattg caaggtgttt ttatgaataa ataattcttc tgagcaattt
    33961 ttgatgcttc cgttattttt actgaagcat gccaaagtct tcttgaacaa atgtacttct
    34021 tcttctgtca ggcttttcaa aatacgtttt acatataatg ttcgttgatc tgacgggatt
    34081 gctggtaaaa cgatcccgag gtctaaatct tcaaaaaagc ggatctcgtt cacagattga
    34141 tggaaagcta tccattcgca aacttgtttg gcatgaagga aagattgcgg tgtctcttgc
    34201 tctgtataac aaagcgaccc aattccaaag tttagctgta actgatacat tttattaaac
    34261 tttgtctgga ttttatcaag caatgatagc aatgaggctc tggaaatccg attgactagg
    34321 ataatgattt gttgtctatg tacagtgagc aaagcctgtc cgtaataaga gaagaccctt
    34381 tcgagcaact ccaataaagg attgatccgt tcttgacaca attgctgatt gtcagaaagt
    34441 tgtcccacaa ctacataata tggagaagaa aaatcaagct caattaagtg agggtggtat
    34501 cgactatgta cttgatatca tcaatgaacc gatggtactt tcttatgcga tggaacaggt
    34561 agagcgattg cttgtttgtg caggaaaaca agcgatgcgg ctgatacgat taggtgagaa
    34621 aataagaaga taaaaataca cggtcccttc atggcgaccg tgcatttttg gtttttatta
    34681 aatatttaat actttattca agaaatcttt tgtccgttca ttttgcggat tttcaaagat
    34741 ttgttgaggc gttccatctt ctaaaaagtt tccgtcgtca atgaacatga cgcgattggc
    34801 cacttcttta gcgaacccca tttcgtgcgt aacgataacc atcgtcatac cttgttctgc
    34861 taactttttc atgacgttca agacatcacc gaccatttca ggatcgagtg cagaagtagg
    34921 ctcatcaaaa agcatgatgt cagggttcat cgctagagca cgagcaatag ccacacgctg
    34981 tttttgtcca ccagaaagca tttcaggata catatctttt ttatcttcta gaccaactgt
    35041 ctgcagtaat ttgattgctt ttttctctgc ttcttctcta gattctttct tcaggtccat
    35101 cggtgctaaa atgatgtttt ccaaaataga aagatgaggg aataggttaa agtgttggaa
    35161 taccattccg atatgttgtc tcaccaagtt gatatctgtg cttttatcca tcaagtgggc
    35221 gccgtcaata ataatttcac cgtcagaagg ttcttctaaa cgattcaagc aacgcaagaa
    35281 tgtacttttt cctgaacctg aagggccaat gatacagacg acatctcctt cgttgatgga
    35341 tacattgatg tcgtttaata ctgtgttatc gccgtatttt ttgactaaat gctcaactaa
    35401 gattttttct gccatcttac ttcaccttct tttctaaaac ttttgctaat tttgtcaatg
    35461 ctgtgatcaa aatcaagtac atgatcgcaa tgatcaagta aacatacgta ctttgtgttg
    35521 tacgtgcaac aatgatctta ccagtttgca gcaattcgac tacaccgatt gcggaaatga
    35581 tcgttgtgtc ttttaatgag ataacgaact gattgacgaa tgatgggatc atgatgcgta
    35641 tcgcttgcgg caaaatgatt ttttgcattg tccgattata agaaagacct aaacttctgg
    35701 aagcttccat ttgtccgaca ggaacggcgt tgatcccgcc tcgaacaatc tctgcgatat
    35761 aagcacttgc gttaagagtc aaggtgataa caccagcaag gaagtcagga atcgtaaatc
    35821 caacgatacc aggcaaccca aagaaaataa agaacgcaag aaccatcatt ggaattcctc
    35881 ggatgatatc gacataaata ctagcaattg tgcgaagagc gcgtattggt gctacactga
    35941 ataatccaaa aatcacaccg attactatag ccaaagcaaa ggagatcaaa gttaggagaa
    36001 ttgttctcca aagtcctttc aacaatgctt gatagttgtt ttggataagt ccaagaaatg
    36061 ttgattcatc agcagtggaa gactcactgg aagtttctga accatccgct acatattctt
    36121 taataatctt atcgtattcg cctgttcttt ttaattcttt caatccttca ttgaacatat
    36181 taagaagctc aggattttgt ccttttttaa cggcaaatgc atattttccg ccagattctt
    36241 ttttgatcgg tgtttccaat tcttgtcctt gtttgatcgc ataaccgatt accggataat
    36301 catccatcat tgcatcgatt gcgccaactt ttaacccgtc gtacaattga tcagcagcat
    36361 caaaatattt gatagaatag ccatattcag attcatggtc gcttaaaaat tcagcacttt
    36421 cggtaccaac ttttgctccg actgtttttc ctttcaaatc ttcataagac ttgatcgaag
    36481 tatctccttt tttgacagct aattggatcc cgctatcaaa gtaagaatca gaaaaatcat
    36541 agcttttctt tcgttcatct gttacagtca ttccggcaat cattccgtct gcttgtcctg
    36601 attccaaagc ttgcaaagca gcagagaaac cacggtagtc cattcggatg ttaaatcctt
    36661 gcagtttggc gatgttttcc atcaatttta catcgatgcc ttcgtactcg ccctttgcgt
    36721 tttgaaattc aaatggagca aaagcagagt cactggcaat tacgtatacg tcttttttgg
    36781 gctctatttt ttgcattgtc tcgcttgtgc ttgactctgc cgttgtagtg ttgtcagctt
    36841 gtgcaacatg accaaacgca aatgtcacaa gtcctgtcat taagaaaaca aatgagtgaa
    36901 ataaatgctt tcttttcata atcaacctcc taaatttcca atattttatt attataggcg
    36961 aaaagtgctg aatcagacaa gactgtaagc tcgaaaaaat gaaaaaaatg cgagtttcta
    37021 acattttatg ttataaaaca taacatgtat aataagataa aaaagtggcg aaagtatgtt
    37081 cggtgtggta taatttttta gtaaggaatg cctttgcttt ccttttcttt ttattggtcg
    37141 taaaatgaaa aggaatgaat gaaaaggagg acgaagaatg attgagagag atacctcccg
    37201 acactttgag cttgtctcga aataccaacc ggctggtgat cagcctgaag cgattaacca
    37261 attagtggat ggcgtagtcg gcgggaaaaa agcacaaatc ttattaggtg cgactggaac
    37321 agggaaaaca tatacgattt ccaatcttat tgagaaagtg aataaaccaa cactgattat
    37381 tgcccataac aaaacattgg ctggtcagct gtacggggaa ttcaaggaat ttttccctaa
    37441 caatgctgtt gaatattttg tttcttatta tgattattat cagcctgaag cttatgtgcc
    37501 ttcaagcgat acgtatatcg aaaaagattc aagtgtaaat gatgagatcg acaaattacg
    37561 acattcggct actagttcat tattagaaag aaatgatgtg atcgtcattg catccgtttc
    37621 atgtattttt ggattaggct cgccttttga gtaccaaaaa caagtagttt cgattcgaca
    37681 gggagcagaa ctagatcgca atcagttgat tcgtgactta gtcagtatcc aattcgaaag
    37741 aaatgatata gattttcagc gcggtcgttt tcgagtgcgc ggtgatgtcg tggagatttt
    37801 tccggcttca agagatgaac gtgccttacg tgtggagttt tttggagatg aggtcgaacg
    37861 tatccgagaa gtcaatgcac tgacaggtga agttttagga gaaacggaac acgtagcaat
    37921 cttcccggcg actcactttg tgacgaatga tgaacatatg gagcatgccg tagcgaatat
    37981 caaagcggaa ttagaacagc gtttaactgt cttgcgaaat gaaaacaaac ttctggaagc
    38041 tcaacgttta gaacagcgaa ccaattacga tattgaaatg atgttagaaa tgggctacac
    38101 gtcgggaatt gaaaattatt ctcggcatat ggacggacgt aaagaaggtg aaccgcctta
    38161 tacgctattg gatttctttc ctgaagattt tttgatcgtg gcggacgaat cgcacgtaac
    38221 catgcctcaa atcagaggaa tgtataatgg tgaccgagcg agaaaacaga tgctagtaga
    38281 ttacggtttc cgtttgccat ctgctttaga caatcggcca cttcggttag aagagtttga
    38341 aaaacatgtc aatcagatca tttatgtctc tgcgacgcca ggaccatatg aacatgaaca
    38401 aacagatacc gttatccaac aaatcatccg tccgacaggg ctgcttgacc cagtgatcga
    38461 agttcgtcca atcatgggac aaatcgatga tctagtaggc gaaataaatg aacgagtcga
    38521 aaaagaccag cgggtgtttg taacgacatt gacgaaaaaa atggcagaag acctcacaga
    38581 ttatttcaaa gaattaggga tcaaagtcaa atatttgcat agtgatatca aaacgctgga
    38641 acgaacagaa atcattcgag atcttcgatt aggtgaattt gatgtgttag ttggaatcaa
    38701 tttgcttcgt gaaggattag acgtacctga agtttccctt gttgctatct tggatgcgga
    38761 taaagaaggc tttttacgta gtgaacgttc cttagtccaa acgattggac gagctgctcg
    38821 gaatgaagaa ggtaaagtaa tcatgtacgc agacaaagta acagattcta tgcgtctagc
    38881 gatggatgaa acgtcaagac gtcgtacgat tcagcagaag tacaatgaag aacatgggat
    38941 cgttccaaaa acgatcatta aagaaatccg agatttgatt tctattacga aagaatcgga
    39001 agatgataca aaagaagcgg tacaagtctc gtacgaagaa atgacgaagg aagaaaaaga
    39061 cacgttattg atgaaattag aaaaagaaat gaaagatgcg gcaaaagctt tggattttga
    39121 aacagccgcg aatgtgcgag atatgatttt agaattaaaa gcttcaaagt aggaggacaa
    39181 catggctaac gataaaatta cgattcatgg tgcacgtgcc cataacttga aaaatataga
    39241 tgtaacgatt ccacgagata aattcgtggt cgttacaggt ttgtccggtt ctggaaaaag
    39301 ttccctagca tttgatacgc tgtacgctga agggcagcgg agatacgtag agagcctttc
    39361 tgcttatgct cgtcagtttt taggacaaat ggataagccg gacgtagata gcatcgacgg
    39421 attgagtccg gcgatttcta ttgatcagaa aacaacaagt aaaaatcctc ggtcgacagt
    39481 aggtactgtg acggaaatca atgactattt acgcttgttg tttgcacgtg taggacatcc
    39541 gatttgtccc aacgatcata tcgagattac cagtcaatca gtagatcaaa tggcagatca
    39601 agtaatggct ttgcctgagc gcacaaagat ccaagtgttg gcaccgatcg tggtaaagaa
    39661 aaaaggacaa cataaaaaaa tattcgaacg aatccaaaaa gagggatacg tccgcgttcg
    39721 agtcgatggt gagatctatg atttaagtga agtgcctgag ctggagaaaa acaaaaaaca
    39781 tgatatcgcg atcgtcatcg accggatcat tgtgaaagaa gggattcgtt ctcgcctatt
    39841 tgattcttta gaggcggccc tgcgattagc agacggctat gctctggttg acgtgatcgg
    39901 tgaagaagaa atgttgttta gcgaacacta tgcctgtcct tattgcggat ttactgttgg
    39961 cgaactagaa ccaagactgt tctcattcaa tgcgcctttt ggagcttgtc cagactgtga
    40021 tggtttaggt gtcaaattag aagtcgatca agatttagtg attccagata gaacgaagac
    40081 acttagtcaa ggtgctatcg ttccatggaa tccaatcagc tctcagtatt atcctcaaat
    40141 gttagcgcag gcttgtgaaa gtttcggtat cgatttagat acaccattcg aagaattacc
    40201 ggaagagcat caggagatca tcttgaatgg gacagatgat cttttccatt tccattatga
    40261 aaatgatttt ggtggcgttc gcgatgtaga aacgactttc gaaggggtat tgaagaatat
    40321 cgagcgtcgt tatcatgata cgaacagtga tttcacgcgt gagcaaatgc gtctttatat
    40381 gacagaatta acttgtcaga catgtaaagg ttatcgattg aatccccaag ctctatctgt
    40441 tcaaatcaat ggaacgaata tcgggcaaac aaatgagttg tctattcaaa aagcagtaga
    40501 tttcttctca aacttaacgt tatctgaaca agagaaagta attgccaaac cgattttaaa
    40561 agaagtaaat gacagacttt cattcttgga aaatgtggga ctagactatc taactttgag
    40621 tcgcgcttcc ggaacattat ctgggggaga agcgcagcga atcagattgg ctacacaaat
    40681 tggatcgaat ctttctggag ttttgtatat tttagatgaa ccatcgatcg gattgcatca
    40741 acgcgacaac gatcgactga ttggctcatt aaagaaaatg cgtgatcttg gcaacaccct
    40801 cgttgtagtc gaacatgatg aagatacgat gtgggcagca gattatttga ttgacgtcgg
    40861 acctggggcc ggagaacaag gcggacaaat cgtggctgca ggcactcctg aagaagtagc
    40921 taatgatgaa aattctttga cagggaaata tctttcaggg aaaaaatcaa tccctgtacc
    40981 aaaagaacgc cgtaaaggta atgggaaagc aatcaagatc actggtgctt cagaaaacaa
    41041 tctgaaaaat attagtgttg aatttccatt aggagaattt gtagcagtca caggtgtgtc
    41101 tggttctgga aagagtacat tggtcaatag tatcttaaag aaatcgttag cgcaaaaatt
    41161 aaataagaat tctgctaagc caggtaaatt caagacaatt tccggctacg aaagtatcga
    41221 aaagatcatc gatatcgatc aaagcccaat cggccggacg ccgagaagta atccagcgac
    41281 ttatacaagt gtatttgatg atatccgtgg gttatttgct caaacgaacg aggcaaaaat
    41341 gcggggttat aagaaagggc gttttagttt caacgtaaaa ggcggtcgtt gtgaagcttg
    41401 tcgcggggat ggaattatta agatcgaaat gcactttttg cctgatgtct atgttccttg
    41461 tgaagtatgt catggtaaac gatataattc tgaaacatta gaagtgcatt acaaaggaaa
    41521 aagcattgct gatattttgg aaatgacagt agaggatgct gtagaattct tcaagcacat
    41581 tccaaagatt catcgcaaac tgcaaacgat tgttgatgtt ggcttaggtt atgtgacgat
    41641 ggggcaacca gcaacgacat tgtccggtgg tgaggcacaa cggatgaaac ttgccagtga
    41701 attgcacaaa atctctaatg gaaagaattt ctatatacta gatgaaccaa cgacaggact
    41761 tcatagcgat gacatcgccc gcttgttgca tgtattacaa agattagtag atgctggtaa
    41821 cacagtttta gtgattgaac acaatctaga tgtaatcaaa acagcagatt atatcattga
    41881 tttaggacca gaaggtggag aaggtggagg aacgatcctt gcgactggaa caccagaaga
    41941 aatcattaac gtaaaagaaa gttatacagg tcactatttg aaaaaaataa tggtataaag
    42001 aggatagata gctgagatag ttaaaaaaac ataaaaaaat gaatttttgc ttgttttatt
    42061 aatatcagtg ttctagaatc attacatggt agttctttct acactgattg gtatagataa
    42121 aaacttccta taagttcaat ccgttcaaaa aagaattgag caatcggagg actttccttt
    42181 gattgctttt ttctttatgg tatgatattc cttgtgtagt tggaaaagaa aaacaagaga
    42241 ttatgagtaa gtgagagata atgatgaaca gtattgaaaa tgaagaattt atccctatat
    42301 tattaggaag cgacatgaac gtttatggta tggcacgttc ttttaatgaa gcatacggca
    42361 aaatctgcca agcatatgct tcggatcagt tagcccctac ccgctacagt aaaattgtca
    42421 atgtagaagt tattccagga tttgataaag accctgtatt tatcgagacg atgcttcgtt
    42481 tggcaaaaga aagatattct gataagagta aaaaatatct tttgattgct tgtggtgatg
    42541 gatatgcaga attgatctcc caacataaac aagagctgtc agaatatttt atctgtccat
    42601 atattgatta ttctttattt gaaaggctga tcaataaagt aagcttttat gaagtttgtg
    42661 aagaatatga tcttccgtat cctaaaacat tgatcgtcag agaagaaatg ttagtaaatg
    42721 gacatttgga acaggaattg ccttttgaat tcccagttgc tttgaaacct gcaaacagtg
    42781 tggagtatct ttctgtccaa tttgaaggaa gaaaaaaagc ttttatttta gagactagag
    42841 aagagttcga tttaatttta ggccggatat acgaagccgg ttataaaagt gaaatgattg
    42901 tacaagattt tatcccgggt gatgatagta atatgcgtgt gctaaatgcg tatgtagatg
    42961 aagatcatca agttcggatg atgtgccttg gtcatccact attagaagat cctacaccag
    43021 catcgattgg taattacgtt gtgatcatgc cagattataa cgaaaaaatc tatcaaacca
    43081 ttaaggcatt tttggaaaaa atcgaatata caggctttgc caatttcgat atgaagtatg
    43141 atcctagaga tggagaatat aaattatttg aaatcaattt gagacaaggt cgtagcagtt
    43201 tctttgtgac attgaacggc ttgaatctgg ctcgttttgt cacagaagat cgagtgttca
    43261 acaaaccctt tgttgaaacg acctatggaa cgaatcaatc agataaagca agattatgga
    43321 tgggtgtgcc caaaaaaatc tttttagaat atgctcggga aaatgaagac aaaaaactag
    43381 ctgagcaaat gatcaaagaa aatcgttacg gtactactgt attttatgaa aaagaccgat
    43441 caatcaagcg ctggttgttg atgaaatata tgttccataa ctatattcca agattcaaaa
    43501 agtattttca tgtgaaagaa ggctagttca tggagaattt tttcagtatt ttaggcggaa
    43561 tgggcacgat ggcgacagaa agttttgttc gcttgatcaa ccaccgaacg aaagctacaa
    43621 aagatcaaga atatttgaat tatgtcttat tcaatcatgc aacagttcct gatcgtacag
    43681 cttatatttt agatcgatca gaagaaaatc caatgccgtt tctgctggat gatattgaga
    43741 aacaaaattt attgcgacca aacttcattg tgttgacttg taacaccgca cactactttt
    43801 ttgaagagtt acaagcggca acggatattc ctatcttgca tatgccaaga gaagcagcaa
    43861 atgaacttgt ccgtcagcat acgacaggga gagtagcgat tctaggaaca gaaggaagta
    43921 tgaaagctgg aatctatgaa cgcgaagtca aaaatcttgg tttcgaaaca gtgattcctg
    43981 atactgcgct tcaagaaaaa atcaattacc tgatctatca tgagatcaag gaatcggatt
    44041 atttgaatca agagctctat tacgaaatct tagaggaagc ggtagagcgc ctaaactgtg
    44101 aaaaagtcat cctaggttgc acagaattat ctttgatgca tgaatttgct gaagataatc
    44161 attacccagt gatcgatgct caatctattt tagcggatcg aacgatcgaa cgtgcattag
    44221 ctgaaagaag cgaggcattg gatacagcat cggaaaagta aaaaaattgg atttggggca
    44281 aatatcctaa aagatatttg gtccaaatct ttttgtaaaa gaaaaagaaa actggtaatg
    44341 atacgcccgg gatgatataa ttttattatg cacaatgtga gaggggttcg taaatatgac
    44401 agataatttg caattagtca tcataacagg tatgagtggt gcagggaaaa cagtagctat
    44461 ccaaagtttt gaagatatgg gctatttttg tatcgataat atgccgcctc gattgattcc
    44521 aaaattctgg gaattgatca aagaatcagg taaagtcaca aaaatggctt taggagtgga
    44581 tttgcgttct cgttccttct ttgaagaaat ccaaaacatg ctgatcgaaa tcgaaaatac
    44641 gaacttcatc gacacttcta tcttattttt agatgcttca gacgaagaac tagtggctcg
    44701 ctacaaagaa acaagacgag cacatccaat ggcgatggac ggtttgatca ctgaagggat
    44761 acgtaaagaa cgggcgatac tagacgactt gaaagcccaa gcttccgtga tcattgatac
    44821 gacaacattg acaccacgac aattacgtga aaaaatcaat gagtcattca aatcaaaggg
    44881 agaccaagga tttcggatcg aatttgtatc atttggtttt aaatacgggt tgccgatcga
    44941 ttcggatatt gtgatggatg tgcgtttttt gcccaaccct cattatatcc cggaactaag
    45001 accattgacc ggaaaagatc agccagtcta tgattatgtg atgtcctttg atgaaacgca
    45061 atcattctac cataaatttt tggatcttct ggaaactgta ttgcctggat acattaaaga
    45121 aggaaaaagc agcttgacag ttgcaatcgg ctgtacaggc ggtcaacacc gttctgtggc
    45181 attgactgaa cgtttggcaa atgctttagg gaaaaactat aaagtcaatg tgacacatcg
    45241 ggacaaggat aaaaggaaag agacggtgaa ccggtcgtga aaatgaaaac ctaccggata
    45301 agaaaaccaa agatcgtagt ggtcggcggt ggcactggtc tgccagttat cctgaaaagt
    45361 ttacgaaatc aaggagcaga tatcacagca gtagtgactg ttgcagatga tggtggaagc
    45421 agcggtgcaa tcagagaatc gatcgctatg gctcctcctg gtgacttgag aaatgtctta
    45481 gtggcgttat cggatatgcc acaattctat gaagatattt tccaataccg tttcaaaaag
    45541 gaagatcagt ttttagccaa tcatacgatc ggtaatctga tcattgccgc agtttccgag
    45601 atgcggggaa gcacttatga ggcgattcaa ttattagcaa aaatgatgca tgtagatgga
    45661 catgtttatc cttcttcaga aactcctttg accttgcatg ctgtttttaa agacggaacg
    45721 actgcagtag gagaatctaa aatcgcgatc aaccgtaaaa ccattgaccg tgtgtatgtc
    45781 tgcaacacaa atgataaaag tgaagccaaa gcggctcgaa aagtcgtttc agcaattatg
    45841 gacgccgaca tggtcgtttt gggacccgga agtttattca ccagtatcct tccaaattta
    45901 gtgataccag aaatcggcga agcaatgctc aagacaactg ctgaaaccgt ctatatctgt
    45961 aatatcatga cccaaaaagg ggaaacggaa catttcacag atgccgatca tatacgtgtt
    46021 ttacacaagc atttgaatca gccttttatc gatacagtat tggtcaatac ggaaaaagtg
    46081 cctgaagatt atatggaccc agagatctat gatgagtatc tagtacaagt cgcacacgac
    46141 ttcaacggat tgagaaatga aggatgtcga gtgatttcca ccgacttttt agaattgaaa
    46201 gatggtggtg tcttccacga tggagacaaa gtagtagaag aattgttccg tttagttttc
    46261 ggttcaaaat attaagcaat ttattgttgg agaaaggagg agaataggat gtcatttgct
    46321 gcagatgtga agaaagaact aactggtttg gaagttcatc gagaacatgc gaaagcggaa
    46381 ttagctgctt tgctgcgaat gaacggctct ttaggtattg tgaaccagca gtttattttg
    46441 aacgtccaaa cagaaaatgc tgcaattgcc agacgtatct attctctttt gaaagatcat
    46501 tatcaggtac gaagtgagct actcgttcgt aaaaaaatga aattaaaaaa gaacaatgtc
    46561 tatatcgtcc ggttgaagca aggcacaaaa gatgtgttga tggatcttga tatcatggat
    46621 ggtatgatgt tcaatggaca cgtttcggac gagattatgg gaaattcgca aaaaatgcgt
    46681 tcttacttac gtggtgcttt tatggcctca ggttcagtca ataatccgga aacgagtcgt
    46741 tatcatttgg aaatattttc tatttatgaa cagcataacc aagacatttg cgatatgtta
    46801 aattattacg gtctacatgc tcgaacgctt gaacggcgca atggctatat ttcgtattta
    46861 aaaggagcag aaaaaattgc tgatttctta acattgattg gtgcaaccaa ctcaatgctc
    46921 cgatttgaag atgttcgaat cgtacgggat atgagaaatt cggtcaatcg tctagtcaat
    46981 tgtgagacag caaatctgaa caagacgatt gatgcggctt ctaaacaaat cgaaaacatc
    47041 gaattgatcg aagcaaaggt cggtctacat gcccttcctg aaaaacttca ggaaatcgca
    47101 gaacttcggc ttcaacatcc agaggtcagc ttgaaagaat taggggaaat gattccctca
    47161 ggtgcaatat caaaatcggg aattaatcac cgaattcgca aaataaatga atttgcggaa
    47221 aaactgcgtg aagaagcagt ttaatccaaa gaaaaaactg atacatgaag caggagatgc
    47281 gaatgtatca gtttttttta aaagctatac ttgcttttct tccgtcaaac tataattcca
    47341 actttactgg agtaacataa tgttgaaagg cttctattgt tttttaatac aatcgtataa
    47401 aaagcgggga gacaactgaa tgaagatttt agttttatac ataaactttt gcttaatata
    47461 gaagtcctga tatagatagg atcaagtatt tatctataac tggagattaa ttaaaaactg
    47521 gaaggaactg ctcgatcaac cttgagaact gtttttctta cttctggtca agattctcgt
    47581 ggttggtgga taaggatggt tgataagttc attggattag ctagtcgaaa acaaccaggt
    47641 gtaattaatg tagaagtttt aacaagggga tggagtgatt tattcagtga taatcataag
    47701 ttcataaata gtaattggga tgtatggttg gtatacgtta tacctgaact tgaactatca
    47761 agatttggtt tagaatttga gaatcgatgt ttataaaggg agggcaaaaa atgtgctttt
    47821 ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat atagcaaagc
    47881 gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata acatttatat
    47941 cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact acagcaccac
    48001 cattgccaga gattacatat ggagaatttc cgtttagtct tgaatatgaa atagatgagg
    48061 aaagaatttt gattgaagat acggtgattg cagaatttat aggaagtttt agagggaatg
    48121 tgacaactca acctagacga agttggagta taaaactttt aaataatagt ggagagttga
    48181 aagcatttca ttatgaattc ttaataatag aaagtgaaga tgtaacaatc actttaagta
    48241 gtggctctgc tggatatttt atgaatgatg gtggaagcaa gccacagctc acagtccgtg
    48301 atcgtattgc aggttttatg catttttctc actatgactt agaagaagct cgtaaaatac
    48361 ttgcagaata tgggatcaca ttaatcaatt gggaatatgc gccaccgata agaaattttt
    48421 tcaatgatta ttattagtct tataaaaaac agaatgtaaa atttatgtgc gcgagtattt
    48481 agttttccat tttttgtttg atagatatat agcattgttg aagcatggga tagtggtgcc
    48541 aattttatca atgaggttgg tcaggcagtt aacataattg gtgatgctat aaatccattt
    48601 aattggaatt ggaggaatta ggtgtggaaa cagtgagatt atggatattc atgaaaaatt
    48661 ttcgttcggc tttgagatat gttattctgg aatcaaaatc aggttattat ctgattgaca
    48721 cagatcacta tactttttgg ggaggctacc tttttccgat ttttaattgg ataatccccc
    48781 ataaggttat gaaaattaac ttgcctgcag aagaaataaa tagattaata ctcaacaaag
    48841 atttacaaag gaaaagagag aaagaacatt ctaatgttgc ttggataggt ccagcatttg
    48901 cagtagtgac ttcttcaatt gtcggtccta tgctagagag tatttatagt gatttttcta
    48961 ttggaatacg ttatttattt gttactttaa gtatagctgt tcttttgtgc ataaagggtt
    49021 atgttagcaa gaagaataaa gatattttaa cactaatcgg tgtaaacaat ttatctagta
    49081 ctaaaattat actgtttcct gaatctataa aagaaggatc aaggttgttg ataattttta
    49141 taatgcttat actgttttct gttttatttg gggtgatgtt tata
//
LOCUS       DARBFD010000010        44939 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000010 DARBFD010000000
VERSION     DARBFD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.010"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(38..1189)
                     /locus_tag="SCK08_000574"
     CDS             complement(38..1189)
                     /locus_tag="SCK08_000574"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEG1200487.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="SCK08_000575"
     CDS             complement(1202..1639)
                     /locus_tag="SCK08_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1200488.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="SCK08_000576"
     CDS             complement(1654..3420)
                     /locus_tag="SCK08_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEG1200489.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="SCK08_000577"
     CDS             3684..4361
                     /locus_tag="SCK08_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1200490.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="SCK08_000578"
     CDS             4364..5491
                     /locus_tag="SCK08_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200491.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="SCK08_000579"
     CDS             complement(5691..6131)
                     /locus_tag="SCK08_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1200492.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="SCK08_000580"
     CDS             complement(6142..8175)
                     /locus_tag="SCK08_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1200493.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="SCK08_000581"
     CDS             complement(8177..9880)
                     /locus_tag="SCK08_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1200494.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="SCK08_000582"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="SCK08_000582"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEG1200495.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="SCK08_000583"
     CDS             complement(10963..11988)
                     /locus_tag="SCK08_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1200496.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="SCK08_000584"
     CDS             complement(12197..13066)
                     /locus_tag="SCK08_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEG1200497.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="SCK08_000585"
     CDS             complement(13525..15417)
                     /locus_tag="SCK08_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1200498.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="SCK08_000586"
     CDS             complement(15414..17141)
                     /locus_tag="SCK08_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1200499.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="SCK08_000587"
     CDS             complement(17157..17807)
                     /locus_tag="SCK08_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1200500.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="SCK08_000588"
     CDS             18011..18307
                     /locus_tag="SCK08_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1200501.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="SCK08_000589"
     CDS             18479..19099
                     /locus_tag="SCK08_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEG1200502.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="SCK08_000590"
     CDS             complement(19191..20357)
                     /locus_tag="SCK08_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1200503.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="SCK08_000591"
     CDS             complement(20369..20722)
                     /locus_tag="SCK08_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1200504.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="SCK08_000592"
     CDS             20850..21872
                     /locus_tag="SCK08_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEG1200505.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="SCK08_000593"
     CDS             21891..22052
                     /locus_tag="SCK08_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200506.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="SCK08_000594"
     CDS             complement(22311..22745)
                     /locus_tag="SCK08_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200507.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="SCK08_000595"
     CDS             complement(22855..24714)
                     /locus_tag="SCK08_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1200508.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="SCK08_000596"
     CDS             complement(24832..25992)
                     /locus_tag="SCK08_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEG1200509.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="SCK08_000597"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="SCK08_000597"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEG1200510.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="SCK08_000598"
     CDS             complement(27739..28437)
                     /locus_tag="SCK08_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1200511.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="SCK08_000599"
     CDS             complement(28394..30055)
                     /locus_tag="SCK08_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEG1200512.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="SCK08_000600"
     CDS             30273..31673
                     /locus_tag="SCK08_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEG1200513.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="SCK08_000601"
     CDS             complement(31925..33004)
                     /locus_tag="SCK08_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1200514.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="SCK08_000602"
     CDS             complement(33090..34049)
                     /locus_tag="SCK08_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1200515.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="SCK08_000603"
     CDS             complement(34061..35572)
                     /locus_tag="SCK08_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1200516.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="SCK08_000604"
     CDS             complement(35594..36157)
                     /locus_tag="SCK08_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEG1200517.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="SCK08_000605"
     CDS             complement(36163..37026)
                     /locus_tag="SCK08_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1200518.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="SCK08_000606"
     CDS             complement(37040..37936)
                     /locus_tag="SCK08_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1200519.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="SCK08_000607"
     CDS             complement(38111..39400)
                     /locus_tag="SCK08_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1200520.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="SCK08_000608"
     CDS             complement(39372..40889)
                     /locus_tag="SCK08_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1200521.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="SCK08_000609"
     CDS             41087..41977
                     /locus_tag="SCK08_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEG1200522.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="SCK08_000610"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="SCK08_000610"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEG1200523.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="SCK08_000611"
     CDS             complement(42886..44358)
                     /locus_tag="SCK08_000611"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEG1200524.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12989 a         9387 c         7783 g        14780 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgttttt
//
LOCUS       DARBFD010000011        43733 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000011 DARBFD010000000
VERSION     DARBFD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.011"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(30..1595)
                     /gene="guaA"
                     /locus_tag="SCK08_000612"
     CDS             complement(30..1595)
                     /gene="guaA"
                     /locus_tag="SCK08_000612"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1200525.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(1654..2577)
                     /locus_tag="SCK08_000613"
     CDS             complement(1654..2577)
                     /locus_tag="SCK08_000613"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEG1200526.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2773..3378
                     /locus_tag="SCK08_000614"
     CDS             2773..3378
                     /locus_tag="SCK08_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1200527.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            3686..4702
                     /locus_tag="SCK08_000615"
     CDS             3686..4702
                     /locus_tag="SCK08_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1200528.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            4843..6144
                     /locus_tag="SCK08_000616"
     CDS             4843..6144
                     /locus_tag="SCK08_000616"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEG1200529.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            6190..6852
                     /gene="deoC"
                     /locus_tag="SCK08_000617"
     CDS             6190..6852
                     /gene="deoC"
                     /locus_tag="SCK08_000617"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEG1200530.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            6873..7271
                     /locus_tag="SCK08_000618"
     CDS             6873..7271
                     /locus_tag="SCK08_000618"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEG1200531.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            7337..8419
                     /locus_tag="SCK08_000619"
     CDS             7337..8419
                     /locus_tag="SCK08_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1200532.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            8631..10193
                     /locus_tag="SCK08_000620"
     CDS             8631..10193
                     /locus_tag="SCK08_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1200533.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            10186..11319
                     /locus_tag="SCK08_000621"
     CDS             10186..11319
                     /locus_tag="SCK08_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200534.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            11319..12275
                     /locus_tag="SCK08_000622"
     CDS             11319..12275
                     /locus_tag="SCK08_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200535.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            12374..13540
                     /gene="deoB"
                     /locus_tag="SCK08_000623"
     CDS             12374..13540
                     /gene="deoB"
                     /locus_tag="SCK08_000623"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEG1200536.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            13564..14382
                     /locus_tag="SCK08_000624"
     CDS             13564..14382
                     /locus_tag="SCK08_000624"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1200537.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            14399..15106
                     /gene="deoD"
                     /locus_tag="SCK08_000625"
     CDS             14399..15106
                     /gene="deoD"
                     /locus_tag="SCK08_000625"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1200538.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(15404..16090)
                     /gene="gpmA"
                     /locus_tag="SCK08_000626"
     CDS             complement(15404..16090)
                     /gene="gpmA"
                     /locus_tag="SCK08_000626"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1200539.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(16262..16843)
                     /locus_tag="SCK08_000627"
     CDS             complement(16262..16843)
                     /locus_tag="SCK08_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1200540.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(16976..17659)
                     /gene="rpiA"
                     /locus_tag="SCK08_000628"
     CDS             complement(16976..17659)
                     /gene="rpiA"
                     /locus_tag="SCK08_000628"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEG1200541.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            17978..18391
                     /gene="rpsL"
                     /locus_tag="SCK08_000629"
     CDS             17978..18391
                     /gene="rpsL"
                     /locus_tag="SCK08_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEG1200542.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            18475..18945
                     /gene="rpsG"
                     /locus_tag="SCK08_000630"
     CDS             18475..18945
                     /gene="rpsG"
                     /locus_tag="SCK08_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEG1200543.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            19039..21126
                     /gene="fusA"
                     /locus_tag="SCK08_000631"
     CDS             19039..21126
                     /gene="fusA"
                     /locus_tag="SCK08_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1200544.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            21298..22485
                     /gene="tuf"
                     /locus_tag="SCK08_000632"
     CDS             21298..22485
                     /gene="tuf"
                     /locus_tag="SCK08_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1200545.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            22892..23200
                     /gene="rpsJ"
                     /locus_tag="SCK08_000633"
     CDS             22892..23200
                     /gene="rpsJ"
                     /locus_tag="SCK08_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEG1200546.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            23231..23860
                     /gene="rplC"
                     /locus_tag="SCK08_000634"
     CDS             23231..23860
                     /gene="rplC"
                     /locus_tag="SCK08_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEG1200547.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            23888..24511
                     /gene="rplD"
                     /locus_tag="SCK08_000635"
     CDS             23888..24511
                     /gene="rplD"
                     /locus_tag="SCK08_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEG1200548.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            24511..24801
                     /gene="rplW"
                     /locus_tag="SCK08_000636"
     CDS             24511..24801
                     /gene="rplW"
                     /locus_tag="SCK08_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEG1200549.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            24840..25673
                     /gene="rplB"
                     /locus_tag="SCK08_000637"
     CDS             24840..25673
                     /gene="rplB"
                     /locus_tag="SCK08_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEG1200550.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            25716..25994
                     /gene="rpsS"
                     /locus_tag="SCK08_000638"
     CDS             25716..25994
                     /gene="rpsS"
                     /locus_tag="SCK08_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEG1200551.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            26017..26364
                     /gene="rplV"
                     /locus_tag="SCK08_000639"
     CDS             26017..26364
                     /gene="rplV"
                     /locus_tag="SCK08_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEG1200552.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            26378..27034
                     /gene="rpsC"
                     /locus_tag="SCK08_000640"
     CDS             26378..27034
                     /gene="rpsC"
                     /locus_tag="SCK08_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEG1200553.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            27037..27471
                     /gene="rplP"
                     /locus_tag="SCK08_000641"
     CDS             27037..27471
                     /gene="rplP"
                     /locus_tag="SCK08_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEG1200554.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            27461..27649
                     /gene="rpmC"
                     /locus_tag="SCK08_000642"
     CDS             27461..27649
                     /gene="rpmC"
                     /locus_tag="SCK08_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEG1200555.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            27674..27940
                     /gene="rpsQ"
                     /locus_tag="SCK08_000643"
     CDS             27674..27940
                     /gene="rpsQ"
                     /locus_tag="SCK08_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEG1200556.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            27998..28366
                     /gene="rplN"
                     /locus_tag="SCK08_000644"
     CDS             27998..28366
                     /gene="rplN"
                     /locus_tag="SCK08_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEG1200557.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            28403..28711
                     /gene="rplX"
                     /locus_tag="SCK08_000645"
     CDS             28403..28711
                     /gene="rplX"
                     /locus_tag="SCK08_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEG1200558.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            28739..29278
                     /gene="rplE"
                     /locus_tag="SCK08_000646"
     CDS             28739..29278
                     /gene="rplE"
                     /locus_tag="SCK08_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEG1200559.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            29297..29482
                     /locus_tag="SCK08_000647"
     CDS             29297..29482
                     /locus_tag="SCK08_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEG1200560.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            29518..29916
                     /gene="rpsH"
                     /locus_tag="SCK08_000648"
     CDS             29518..29916
                     /gene="rpsH"
                     /locus_tag="SCK08_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEG1200561.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            29948..30484
                     /gene="rplF"
                     /locus_tag="SCK08_000649"
     CDS             29948..30484
                     /gene="rplF"
                     /locus_tag="SCK08_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEG1200562.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            30645..31001
                     /gene="rplR"
                     /locus_tag="SCK08_000650"
     CDS             30645..31001
                     /gene="rplR"
                     /locus_tag="SCK08_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEG1200563.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            31022..31522
                     /gene="rpsE"
                     /locus_tag="SCK08_000651"
     CDS             31022..31522
                     /gene="rpsE"
                     /locus_tag="SCK08_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEG1200564.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            31537..31716
                     /gene="rpmD"
                     /locus_tag="SCK08_000652"
     CDS             31537..31716
                     /gene="rpmD"
                     /locus_tag="SCK08_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEG1200565.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            31760..32200
                     /gene="rplO"
                     /locus_tag="SCK08_000653"
     CDS             31760..32200
                     /gene="rplO"
                     /locus_tag="SCK08_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEG1200566.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            32200..33495
                     /gene="secY"
                     /locus_tag="SCK08_000654"
     CDS             32200..33495
                     /gene="secY"
                     /locus_tag="SCK08_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEG1200567.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            33557..34204
                     /locus_tag="SCK08_000655"
     CDS             33557..34204
                     /locus_tag="SCK08_000655"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEG1200568.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            34395..34613
                     /gene="infA"
                     /locus_tag="SCK08_000656"
     CDS             34395..34613
                     /gene="infA"
                     /locus_tag="SCK08_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEG1200569.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            34646..34762
                     /gene="rpmJ"
                     /locus_tag="SCK08_000657"
     CDS             34646..34762
                     /gene="rpmJ"
                     /locus_tag="SCK08_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEG1200570.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            34780..35145
                     /gene="rpsM"
                     /locus_tag="SCK08_000658"
     CDS             34780..35145
                     /gene="rpsM"
                     /locus_tag="SCK08_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEG1200571.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            35173..35562
                     /gene="rpsK"
                     /locus_tag="SCK08_000659"
     CDS             35173..35562
                     /gene="rpsK"
                     /locus_tag="SCK08_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEG1200572.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            35642..36580
                     /locus_tag="SCK08_000660"
     CDS             35642..36580
                     /locus_tag="SCK08_000660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEG1200573.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            36608..36988
                     /gene="rplQ"
                     /locus_tag="SCK08_000661"
     CDS             36608..36988
                     /gene="rplQ"
                     /locus_tag="SCK08_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEG1200574.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            37260..38099
                     /locus_tag="SCK08_000662"
     CDS             37260..38099
                     /locus_tag="SCK08_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1200575.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            38075..38944
                     /locus_tag="SCK08_000663"
     CDS             38075..38944
                     /locus_tag="SCK08_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1200576.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            38934..39731
                     /locus_tag="SCK08_000664"
     CDS             38934..39731
                     /locus_tag="SCK08_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1200577.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            39934..40680
                     /gene="truA"
                     /locus_tag="SCK08_000665"
     CDS             39934..40680
                     /gene="truA"
                     /locus_tag="SCK08_000665"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1200578.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(41013..43682)
                     /locus_tag="SCK08_000666"
     CDS             complement(41013..43682)
                     /locus_tag="SCK08_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEG1200579.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        14003 a         8121 c         9484 g        12125 t
ORIGIN      
        1 gtataagagg atttctagct ctcctacaac tattcccact ccactgttgc tggtggttta
       61 gaagtgatgt catacacgat acggttgacg tgggctactt cgtttacgat acgtacagag
      121 attttttgca agacttccca tgggatacgg gcaaagtcag cggtcattcc gtcgattgaa
      181 gttacagcac ggataccgac tgtgtagtcg taagtacgtc cgtcacccat aacaccgaca
      241 gagcggatcc ctggaagaac agtgaaatat tgccagatat cacggtcaag tccagcagct
      301 gcgatttctt cacgcaagat agcatcagat tcgcgtacga tttctagctt gtcttcagtg
      361 atttcgccaa gaacacgaat accaagacca ggaccaggga atggctgacg ccaaacgatt
      421 gaatcaggca tgcctaactg agtccctaat tcacggactt cgtctttgaa taatgtgttt
      481 aatggttcga tcaattcaaa ttgcatatct tctggcagtc cacctacatt atggtgagat
      541 ttgatcgtct gtgctgtttc tgtaccagat tcgataacgt cagtatacaa cgttccttgt
      601 gctaggaaag agacgccttc ttcaccagca agttttgttg cttcgtcatc aaaaacatat
      661 acgaattcat tcccaatgat tttacgtttt tgttctgggt cagatacacc tttcaacttg
      721 tctaagaagc gatctcttgc atcgactttg atgatgttca agccgaattt tccgcctaga
      781 ctttccatta cttgttccgc ttcgcctttg cgtagcagac cgtggtcaac aaagatagat
      841 gtcaattggt caccgatcgc tttttgtaaa agaaccccaa cgacactaga atcaacgcca
      901 cctgaaagac caagcaagac ttttttgtca ccaacttgtt cacggatttt ttctacttgc
      961 atatcgataa agttatccat tgtccagtta ccttcacagt gacaaacatc aaaggcaaag
     1021 tggcgcagca attcgattcc gtattcggaa tgacgtactt ctgcatggaa ttggatacca
     1081 taaaaattac gtgttgtgtc ttggatagaa gcaatcggac aatctttgct tgttccgact
     1141 gtttcaaagc cttcaggaac tttagttacc aagtcaccat ggctcatcca tactgtttgt
     1201 tgttttggtg tttctttaaa caagacagct tgatcgcttg ttacttctaa caaggctttc
     1261 ccatattcgc ggtttttagc aggctctacg gaaccgccaa gtttgtacgt gatcaattgc
     1321 ataccatagc aaatccctaa tacaggaata cctaaattga agatctcagg atcgatccca
     1381 aatgcattct catcatatac actgtttggt ccgccagaaa gaatgattcc ctttggattc
     1441 atcgctttta cttcttctgc agtaatgcgg tggctcaata attcggaaaa gactccaaga
     1501 tcacgaatac gtcttgtgat caattggtta tactgactac cgtagtctaa tacgataatc
     1561 ttttcaacgt tcgtcatatc ggcaacgttt gtcaaattta ttacccctat ccctttaaat
     1621 tttatctcaa aatagtcaaa ctattttaaa atgctaatat tttcgcatga aaatcgaatc
     1681 gatgatatga ttttcagttt tatggagaat tatatcggct cttcctcgtg taggcaagat
     1741 atattcctgt aaatttttca aattcacatt tttccataca tcttttgcca ttttaaaggc
     1801 ttcttcacga tctccgatag caaaacgata ataataattc tctggatttt ggaaagccgt
     1861 atccagtaac gcaccaaaac gatccaaata ccatttttcg atcagcacgg gatcggcatc
     1921 gacatagaca gaaaaatcaa aaaagtcgct gacgtagatt tgctgattcg caggtaattg
     1981 aagcgtattg atcccctcaa cgatcaagat atcaggctgc tggatcaatt cgtacttgtc
     2041 tggaatgata tcatacacat catgtgagta aacaggtact tttatctctt cctgaccaga
     2101 ttttacacgg ttcaagaaat cgatcagcat ctccatatca taactttcag gaaatccttt
     2161 acggtccatg attccacgct cttttaatat acgatttgga taaagaaatc cgtcagtcgt
     2221 gatcagctga acgtttcttc gtttgaaggt acgagacaaa atcgtctgca gcagtctggc
     2281 tgttgtactt tttccaacgg ctacacttcc ggcgatcccg ataataaaag gcggtacttc
     2341 tacgtattta tgaagaaata aacctttact caagaacaaa gattcaaatt ctttcatata
     2401 aagataaatc agatgagcaa gaggtacata gatatctcga acatcctgca tggaaatgcg
     2461 gtcattcaaa cttttgatat tatctagttc atcttgtgtc aaaggtgctt gcccattacg
     2521 ataaaattct cgccattctt ctcttgccac acggtagtag ttcattggat cattcatatt
     2581 agcctcctgc gttctcccat caatggaact attttaacac aaaataaacg caaaccccac
     2641 cacttaagag agattgccga tctttttgta taatctttat aatagaaaga aacggagtct
     2701 tattcttgac ttgcgcacgg atacataaaa ggatagactg ttgtcatgaa agaaatgaga
     2761 aggatggaag acatgacaaa tcattactat tctgaacgcc cagatacggc gcacgatttt
     2821 gaacaatggg cttttgagtt aaaaggcaga acctttcaat ttgtaacaga ttctggtgta
     2881 ttttcacgtg aaacagtgga ttatggttca cgtgtcctga ttgatgcttt cgattgggaa
     2941 gagttacctg atgaaggaaa gatcttagat gtgggatgcg ggtatggacc gattgggtta
     3001 gctttggcat ttgcgacaca gcggtttgtt gaaatggtcg acattaatga acgagcggta
     3061 agtctagcgc aaggaaacgc aaagcgtaac caaatcgaac atgttgatat ccatcaatcg
     3121 aatatctatg aagcagttca tgaagaaacc tatgcagcta tcgtaagcaa tccgccgatc
     3181 cgtgctggaa aaaaagttgt acatgaaatt ttgacaggcg catatcctcg gttgaagaag
     3241 ggcggtacac tgacaattgt gatccagaag aaacaaggag cgccgagtgc gcaaaagaaa
     3301 atggaagaaa catttggtaa tgcagaaatc gtcacaaaag ataaaggata ttatatcatc
     3361 aaaagtgtga aagaataact agctgaaaaa tagtggagcg acgaaagagt ggaactgggc
     3421 gtgcatgtac gggtacgtat cagttctttt tttgttgaga agtttttaaa aacaaagaac
     3481 tattttttct tcaaaaagaa atagtaggaa cagagtcgtt gttttttacg ataaaataaa
     3541 taagatacag aacttgtata gaaggctgcc aagtcggtag ataaaggagg tttttatcaa
     3601 aaaacatact ttcctttttt ctctattctt ttcttggggg ttttggtaca ctgataggga
     3661 atttagatag aagggagcca ataggatgaa gctgaccatc agagatatcg cagaaatggc
     3721 aggcgtatca gtaacaactg tctcacagat tttgaataac aaaggaagca gatttagtga
     3781 taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat cattataagc ccgattattt
     3841 cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga atgatcgtcc ctgatgtcac
     3901 tgatttcttc ttctccaaac tgatcgaagg tgtagagagc tatttgaatc ctttgggcta
     3961 tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat gagatcaagt atttacaaga
     4021 gctttcccac cgctcagtgg acggaatctt gctggccaca ccaaatattt tacctgagaa
     4081 atatgcatta gacagcggtt tttatcaaaa aatgccaatc attttaatcg atcgcggatt
     4141 gaatcgtcgg gataacggac ggcttatcgt aaaggaatat gagggcgcct atcaagctgt
     4201 ttcgctgttt attgaaaatg gccatacgaa gatcggcatg ctgaaggaaa ccactggata
     4261 ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg ctgaaagatc atggcctgcc
     4321 ttttaatggg aaatttgttg aacatggaga gttgactgtc caaggtggat atactgcatc
     4381 aaaaaaatta ctcaaacata aagatatcac ggctattttt tgcgggaatg atgccatggc
     4441 gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa atcccagaag atatctcagt
     4501 cataggattc gatggattaa agctgtcaga atacatgatt ccgcaattga cgacagtgca
     4561 gcagccaagt tttgatattg gattttatgc tgcacgtttc ctgatcgata cgatcgaatt
     4621 tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa ttaattattc gtgagagcgt
     4681 aaaagtattg acaaagggct aactcggcgg tatattgctt atgtaagcat ttacaaaggc
     4741 tagttagtgc tttttttatt tttagtaaac cagtttacta aagtggttta cctttcctaa
     4801 aaataatgta taataccgaa tgaagacaag gaggatcagg caatgcgaat ggtggatctg
     4861 attgaaaaaa agagggatgg aaacgagctg tcgaaagagg aaatcgagta catcgtcacg
     4921 aactatacga atggaaagat tccagattat caagtcagtg cattattgat ggcaattttt
     4981 tatcaagaca tgacaaatga agaaatcacg aacttaacat tggcgattgc taattctggt
     5041 gatgtcatcg atttgagttc tcttgaagga ataaaggtag ataagcattc gacaggtggc
     5101 gttggtgaca cgaccacatt gattttagcc cccctagtag ctagtgtcgg agtgacagtg
     5161 gctaaaatgt ctggacgcgg tctaggttac acaggtggta cactagacaa gctagaagca
     5221 attcccggat ttcagatcga gctttcggat gaagaatttg tacgcattgt taatgaaagt
     5281 aaagtagcgg taattggtca atcaggaaac ttggcaccgg cggacaaaaa attgtatgcc
     5341 ttgcgtgacg tcacagcgac cgttgattct cttccgctga ttgccagttc gattatgagt
     5401 aaaaaaatcg cggcaggagc agatgcaatc gttttggatg tgaccactgg agacggtgct
     5461 ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga ccatgacgag cattgggaaa
     5521 ctggctaacc gagaaacagt ggcggttatc tcagatatgt cagaacctct tggcgaagct
     5581 atcggaaata gtctagaagt tgtcgaggca atcgaaacat tacaaggaaa tggtccagaa
     5641 gatctagtag aaatgtgcta cgccttagga agccaaatgg ttgttcttgc tggcaaagct
     5701 aagacaaccg atgaagcaag aacgttgctt caagaagctc tggaatcagg taaagcgtta
     5761 gcaaaattca aagaaatgat ccaaaatcaa gggggagacc cagcgatcgt ggaacatcca
     5821 gaacgaattt tgactgcacg ctacacaatg gagcttcctg ctaaacaaag cggagttgtg
     5881 tcaaaaatcg tggcaaatga attaggaatt gctgcaatga tgctaggtgc tgggcgtaaa
     5941 accaaagaag aagatattga tcacgctgtt ggattgaaac tgcataaaaa aattggtgac
     6001 actgtgacaa aaggtgagtc actattgacg atctatagta acgacgaaga aatcacttct
     6061 gtcattgaat tattatataa aaatattgaa attggtgaat cggcaatgaa gccaacgttg
     6121 attcatgaca tcatcacaga ataaaggaaa aaagcggact ttaccgtttt aaagaaaagg
     6181 ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta ttttgaaagc agacgcatct
     6241 aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat accattttta ttctgtctgc
     6301 atcaatccag catgggttgc gctagcgaaa gaacaattac aaggtgagcc agtagccgta
     6361 tgtacagtaa tcgggtttcc tctaggagca aacacacccg agacaaaagc atttgaaaca
     6421 acagatgcca tcaataacgg tgcagatgaa gtcgatatgg tcatcaatat cggtgctttg
     6481 aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag cagttgttga agcagcaaaa
     6541 gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat tagatagaga agaaatcatc
     6601 cgagcttgcg aaatagcaaa agctgcaggc gctgattttg tcaaaacatc aactggtttc
     6661 tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc gtgaaactgt aggaccagaa
     6721 atgggtgtca aagcttccgg cggtatccat aacgaagaag aagcaatggc aatgatcgaa
     6781 gctggtgcta caagaatcgg aacaagcgca ggagtagcaa tcgtatctgg ttcaactggt
     6841 gaaggctatt aattttttca aaggagaagc tcatggcaga actcaaacaa gaatggatca
     6901 acacagctat tgaagcgtta gataaagcct atgttcctta ttcccatttt ccggtaggcg
     6961 cttgtctcgt aacggaatca ggcaagattt atcaaggcat caacattgag aatgcatcat
     7021 ttggattaac gaattgtgcc gagcgcacag cttttttcaa agcagtatct gaaggagaaa
     7081 aaacatttac gcatctagtc gtagcggggc acacccccga tcctatctct ccttgtggtg
     7141 catgtcgaca agtaatggca gaattctgtt cccctgatat gccggttacg ctagtaggag
     7201 acaatggtgt gacgaaggca acgaccgttc gtgcactgct gccttatgca tttactgaaa
     7261 aagatctata actcatgttc attcagtcag ctgactgttg aaaaataaaa caattatttt
     7321 aggaggcttt acagaaatga aaaaagcaaa attatttggt ttaggtgctg tcgcactagc
     7381 agcagggtta tttttaggtg catgcggcaa caacggctct actgattcat ccggtggcaa
     7441 aagctcatca gacacaacaa ctgcagcatt gatcacagac acaggtggtg tagacgaccg
     7501 ttcattcaac cagtcagctt gggaaggact agaaaaatgg gggaaagatc aaggactttc
     7561 acgaggaaat gatggcttcc aatatttcca atcttcaaat gaatcagatt acatcccaaa
     7621 tattgaccaa gcattgaatg ctggattcaa aacgattttt ggtattggtt ataaactaaa
     7681 accagcgatc caagaacaag caacaaacaa tacaggtacg aactttgtta ttatcgatga
     7741 tgtcatcgat ggattagata acgttgtatc tgcaactttc aaagacaacg aagcttctta
     7801 tcttgcaggt gttgcagcag cttatacgac tgaaacaaac gtagtcggct ttatcggcgg
     7861 tgtaaaaggt gaagtaatcg atcgttttga tgctggattc aaagccggtg ttgatgcagg
     7921 tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa tatgctggtg acttctctgc
     7981 tccagataaa ggacgttcaa ttgctcaagg tatgtatgca caaaacgcag atatcatctt
     8041 ccatgcttca ggcggtacag gtaacggtgt attccaagaa gcaaaatcat tgaacgaatc
     8101 aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac caatcagacg aaggcgaata
     8161 cacattaaac ggcgaaaaga aaaacttcac attgacatct acactgaaag cagttggaac
     8221 agtagtagaa gatttggcac aaaaatcagc ggacggtaaa ttccctggcg gcgaacacac
     8281 tgtttatgga ttgaaagaag acggtgttgg tctaacagaa ggacaacttt ctgacgaagc
     8341 gaaaaaagca gttgatgaag caaaagaaaa aatcatttca ggtgacgtaa aagttccaga
     8401 aactcctgaa gaaaactaaa accatttcta tagccttgtt agacacatgt ttgacaaggc
     8461 tatggtttga ttagaacaag aattgttcat ttatccaagc tgccttgtga cagtttggat
     8521 aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa gaacgctcac taagcgtgaa
     8581 tcttagtcaa tcgttgccct agatagaaat cgttaggatg tgaaaaaaag gtggttcaag
     8641 aaaatattgt catagaaatg cagaatatta ccaaagaatt tggaacattc aaggcaaatg
     8701 acaacatcaa tttacaagta aaggcaggag aaattcatgc gttgcttgga gaaaacggtg
     8761 ctggcaaatc tacattgatg aacgtgcttt ccggattatt agagccgaca tcagggaaaa
     8821 ttttgatgcg tgggaaagaa gtaaagatca caagtccgac caaagccaac caattaggga
     8881 ttgggatggt ccatcagcac tttatgcttg ttgatgcctt tactgtaaca gaaaacatcg
     8941 tgttgggaag cgagcctagt cgcgcaggga tgcttgacca taaaaaagca cgaaaagaga
     9001 tccaaaaagt ttctgaacag tatggattgt cagtcaaccc agatgcttac gttcgtgata
     9061 tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac actttaccga ggagcagatg
     9121 tactgatttt tgatgagccg acagctgtat tgacccctca agaaattgat gaattaatcg
     9181 tgatcatgaa ggaattagtc aaagaaggca agtcgatcat tttgattacg cataagttag
     9241 atgaaatcaa agcagtagct gaccgctgta cagttatccg ccgtggaaaa ggaatcggta
     9301 cagtcaacgt taaagacgta acctcacaac aattagccga tatgatggtt ggaagaacgg
     9361 tttcattcaa aacgatgaaa aaagaagcga agcctcaaga agtcgttttg tctattgaaa
     9421 atctagtggt gaaagaaaat cgtggattag aagccgtgaa aaacttgaac ttagaggttc
     9481 gtgctggcga agtacttggt atcgctggaa tcgatggaaa cgggcagtcg gagttgatcc
     9541 aagctttgac tggtttgcga aaggcagaaa gcggacatat caagctaaaa ggggaagaca
     9601 tcactaataa aaaaccccga aaaatcactg aacatggtgt aggacacgtg ccagaagacc
     9661 gtcataaata cgggttagtc ctagatatga cattatctga aaatatcgca ctacaaacct
     9721 atcatcaaaa accttacagt aaaaacggca tgctgaatta ttcagtaatg aatgaacatg
     9781 ccagagaatt gatcgaagaa tatgatgttc gaacaacgaa tgaacttgtt cccgcaaaag
     9841 ctttatcagg cggaaatcag caaaaagcaa ttatcgctcg tgaagtcgac cgagatcctg
     9901 atctgttgat tgttgcaaat ccaactcgtg gactggatgt aggtgcgatc gaatttattc
     9961 ataaacgtct gatcgaacaa agggacaaat acaaagcagt gttactgatc agtttcgaat
    10021 tagaagaaat tttaaatgtt tcggatcgta ttgctgttat ccatgaagga gaaattgtcg
    10081 gtatcgttga tccgaaagaa acatctgaaa atgaattagg tctattgatg gcgggctata
    10141 ccttagaaga agcacgaaaa gagttagaaa agggggctgg cgcacatgaa tgatcgcagc
    10201 gaaaaattcc gtaatttatt agtgcctgta ctttcagttg ttttaggatt gatcttaggc
    10261 gcgatattga tggctgcttt tggttttcag cccatccaag gttattcttc tatgctagat
    10321 gcagcccttg gttcgcaaag agggataggt gaaacgttaa ggcaagcaac gccgttgatt
    10381 tttacagcat taggtttttc tgtagctaac tcagctgggt ttttcaatat tgggctttct
    10441 ggacaagcac tttgcgggtg gatcgtcagt gtctgggtgg cacttgcgtt tccagatctg
    10501 ccaaaagttg ttttgcttcc gttatgcgtg atcttaggtg ccttagctgg tgcgatggca
    10561 gcggctgttc caggtttact tcgagcattt tttggaacaa gtgaagtgat cgtaacaatc
    10621 atgatgaact atatcttatt atacgtaagc acatttgctt tacatgaagt gatgcctgaa
    10681 agctatcgct caagtttgga ctcttccaat atgatcagtc aaaatgcctc actgcggtta
    10741 ccatggttaa cacaagcttt tggcggctct cgcgtaaact caggattatt tttagcaatc
    10801 atcgcgctta ttttcgtttg gctgctgatg aagaaaacga ctttaggatt tgaaatccgt
    10861 tctgttggat tgaatccttt tgcttctgag tatgcaggaa tgagtagtaa acgtacgatc
    10921 gttttgtcga tggtcatttc tggcgctttg gcagggcttg gcggtgtggt tgaaggatta
    10981 ggaacgtatc aaaacttctt tgttcaaacg acttcgctat ctattggatt tgacggaatg
    11041 gctgtttcct tgcttggttc tggatcagca atcggtattc ttttatcagc gatcttgttc
    11101 agtattttga agatcggtgg acttggcatg cagacaggag caggtgttcc gtttgagatc
    11161 gtcaatgtat cgattgcttt gattatcttc tttgtcggga tcaacttcct gattcgtttc
    11221 gtcatggcta aagtcttcca agggaaaaaa caagaagaga tcgttgcgac aatagaatcg
    11281 aaaccaacga atcaaaacca tgaagggggc gaactataat gtcgactatc gaattaattg
    11341 ccttgatcct tacttctaca ctggtttatt caacaccgct aattctaacg tcactaggcg
    11401 gaactttttc tgaacgaagc gggatcgtaa acgtagggct agagggaatc atggtcatgg
    11461 gtgcttttag ttccatcgtt ttcaacttga cgatgagtcc atcacttggt tctatgacac
    11521 catggatcgc tattttagtg ggtggcgtcg taggtatcct attttctctg attcacgcag
    11581 tagcaacagt cagtttgcgt gctgaccata ttatcagcgg gacagtaatt aatctgatgg
    11641 cacctgcttt aggtgttttc ttgatcaaag catggtatgg taaaggtcaa actgataaca
    11701 tcacgcagaa tctggggtat ttttctttcc caggattagc agatattccg gtcattggac
    11761 caattttctt taaaaataca ttcttacctg cttattgtgc tattttgatt gctgtcctag
    11821 cttgggtgat catcttcaag acgaaattcg gcttgcgcct ccgttcagtt ggtgaaaatc
    11881 ctcaagctgc cgacacatta ggtatcaatg tatatgctat gcgttattca ggcgtactga
    11941 tttctggctt gttaggcggt atgggcggtg ctgtttttgc tcagtcgatt tccggtaact
    12001 tttcagctgg tacgatcgtt ggacaagggt tcatctccat ggctgctatg atctttggta
    12061 agtggaatcc tttaggggca atgggtgcct cattgttctt tggttttgct caaagcttga
    12121 gtatcattgg cgcacaatta ccagttattt cttcgattcc gccagtattg ctgcaaattg
    12181 ctccatattt gctgacaatc attgtgctgg ttgttttctt agggaaagct tctggtccaa
    12241 aagcaaacgg gaagaactat atcaaatcaa aataatcttt tagcgaataa aaagggtggg
    12301 ttacgggaag tgtttttcgt ctcgcccttt ttgttttaaa ggcaataaaa ataaaaagaa
    12361 agaaggaata atcatgttta aacgcgtaca tctgatcgtc atggattcag taggaattgg
    12421 agaagcacca gatgccgata agttcggtga tgttggttct gatacattag gacacattgc
    12481 gaaagaagca ggattgaacg ttccacattt agagcagctt ggtttaggaa cgatccgtcc
    12541 tcttgaaggt gtggaatcag tgaaagatca tcaaggctat gcgacgaaat tagaagaagt
    12601 ttctgtaggg aaagatacga tgacaggcca ctgggaaatc atgggattga atatcaaaac
    12661 tccatttcgt gtgtttccta atggattccc tgatgatctg atcaaacaaa tcgaagaatt
    12721 ttctggccgt aaagtcgtat gtaacaaacc ttatagcgga acagctgtta ttgacgatta
    12781 cggtgaacac caaatgaaaa caggcgattt aattgtatat acttctgccg atcctgtcct
    12841 tcagatcgcg gcacatgaag aaatcattcc tttagaagaa ttatatcgga tttgccaatt
    12901 cgttcgtgat ataacaaaag atgatccgta tatgattggc cgaatcattg cacgtccgta
    12961 cgtgggtgaa ccaggagact tcacacgaac aagcaatcgc catgattatg cgttgaatcc
    13021 attcggacac acagtccttg attctttgaa agaagcagga aaggacgtga ttgctgttgg
    13081 aaagatcaac gatattttca acggacaagg aatcactgaa tctgtccgca cgaaaagcaa
    13141 tatggatggc gttgaccagc tgctgaaagt tatgaaaaac gatttttcag gaatcagctt
    13201 taccaattta gtagattttg atgcattata cggtcatcgt cgggatgtag tcggctatgc
    13261 gcatgcgatt gaagaatttg acttacgaat tccagaattg cttgaagcga tggaagaaga
    13321 cgacttgcta atgattacag ccgatcacgg aaacgatccg acgtttactg gtaccgatca
    13381 tacgcgagaa tatgtaccgt tattggttta cagtaaaaaa atgaaagaac aaggggcatt
    13441 gcctcaagga tattattcag atatttcagc aacgattgct gagaacttta gtgtttcttc
    13501 gactgaaaat ggcgaaagct tcttgaaatt attaaaataa tctttttaaa taggagaaag
    13561 caaatgacca agttaagcga gatgttacaa gaaacgaccc agtttatcct tgataaagga
    13621 gtaaaagaaa ttgattttgg tttgattcta ggttcaggat taggtgaatt agcagatgaa
    13681 atcgaagatg ctgtaatgat ttcttatgat gagatcccgt tctttcctgt ttcgacagta
    13741 gttgggcatg caggaaagct agtttatggg tctctggcag gcaagaaagt gttagccatg
    13801 caagggagat tccactacta tgaaggtcat tccatgcaaa cagtgaccta tccagtacgt
    13861 gtgatggccg gactaggggc ccattcagtc attgtgacta atgcatgtgg tggtgtcaac
    13921 gaatcatttg taccaggaga tttgatgctg attaccgatc atatcaattt tactggtgaa
    13981 aatcctttga tcggtccgaa tgaagatgca atgggcccta gatttccaga tatgagtcaa
    14041 gcttatacgc ctgactatca agcaacagca aaagcagcag cagaaaaact aggtttggat
    14101 cttaaagaag gcgtatatat gggctattct ggaccaactt atgaaacgcc tgcggagatc
    14161 cgaatgtctc ggacaatagg agccgatgca gtagggatgt cgactgttcc agaagtcatc
    14221 gtagcagctc acagcggatt aaaagtgtta ggaatctctt gtattaccaa cttagcagca
    14281 ggtatgcaag cgaatctgag ccatgaagaa gttgtagaaa caactcagag agtcaaacaa
    14341 tcttttaaag cattggttaa agaaacattg acattattgt aataggagga acaataaaat
    14401 gagtgtgcat attgaagcga aagaaggcga gatcgccgat aagattttac ttccgggaga
    14461 tcctttgcga gctaaatata ttgcggagac ttttttggaa gatccagttt gttacaatca
    14521 ggtacgcggt atgttaggct ttaccggcaa atataaagga gaacgtattt ctgttcaagg
    14581 aacaggaatg ggaatgccat cagccaccat ctatgcccat gaattgatcc agtcgtatgg
    14641 tgtaaagaaa ttgattcgag taggtacatg cggtgcattg tcaaaagacg tccatgttcg
    14701 tgatttagta ctagcacaag gtgcagcgac cagttcttcg atgatcgaaa aaaatttcca
    14761 agcctttcat tttcctccaa tcagtgattt caatctatta ctgaaagcat atgagatagc
    14821 taaagaaaag ggctatacgg ttcatgtagg aaatgttctt tcggaagatt ctttctacaa
    14881 agatgatttg acagagacgt tccaattagc tgaacttggc gttttaggag tggaaatgga
    14941 agcagcggct ctttattact taggcgcgaa ataccatgtg cagacattaa gtttgatgac
    15001 cgttagcgac catttgatca ccggtgaaga aacaacagct gcagaacgtc aatctacgtt
    15061 caatgaaatg atcgaagtag gattggaaac agcgatagca gaataagaag gaaatgaaaa
    15121 agctgccctg cattaagtaa taagaacatc caagcaaaaa tagctcctca tgtttttgac
    15181 ttggctggac ttattattgc aaatgtaagc ttttgaacac cgtttaccaa gaggttgtga
    15241 atctgccgtt taactctgag tcacaaggaa caatttcaaa aaacagcttt tcatgtttga
    15301 tgaatattgt gacttgtgac cgagaagttg gcaggagaaa accgtttata cgaaaaaagg
    15361 gactgtgaca aatcgtgtca cagtcccttt tttatagtgt tttttatagg tagtattttt
    15421 tagcaactgt taagtcatca tttaattcat aaacaagtgg ttgacctgtt ggaatttcaa
    15481 gatccatgat gtcttcgtca gagatacctt cgatatgttt agctagggcg cgtaatgagt
    15541 taccatgtgc agctactaaa acagttttgt tgtctagtaa tgctggagca atttcatctt
    15601 gccagaatgg caaagcgcgt tctaatgtca ctttcaagtt ttctccacca ggaatatcgc
    15661 gtttgtctaa cattgcgtaa cgacggtcat ttgctgcaga accttcgtca gtttcttcca
    15721 ttaatggagg aagagtatcg taagaacgac gccagatgtg cacttgttcg tcgccgtatt
    15781 tttcagctgt ttcttttttg ttcaaacctt gtaatttacc atagtgacgt tcgtttaagc
    15841 gccatgattt gatttgtggt acccaaagtt gatctgaata ttctaagatc aagttacatg
    15901 ttttgattgc acgagttaaa acagaagtgt aagcaacatc aaattcgatt ccagcttctt
    15961 tgattttgcg tccgccttct tttgcttctt cgattccttc tggtgctaga tttacatcag
    16021 cccaaccagt aaattggttc aatgcattcc attcactaag accgtgacga gaaaaaacta
    16081 attttggcat gtttagctct ccttttaaga taggtttata tttctgaaat tttcagttct
    16141 gttttattgt acaatttttt ggaagagatt tccatcgaaa aattagataa gattctatga
    16201 gaaaacaagt agagaatata aaaaaacgaa cagtgcccac gcaactgttc gtttttaggt
    16261 attagatgca taattgctcg atttcttttt caggtaaatt gatatattta taacatgttc
    16321 caactgaaac accgcatttt gaagaaataa attggatcgt ttcttttttt tcataataga
    16381 gacgtcggat ttttttgatc gtttttgcat cgatttttgg tcttccgccg atttttcctt
    16441 ttttacgagc ttcatctaat ccaactaatg tacgttcttt gatcaatgcg cactccatgt
    16501 ttgctaggtt ttccattaat tggaaataga tttttcccat ttcgcgcgta tcgattcctt
    16561 catcaagact aatcaagtgc agcccgcgat ctttgaataa ttgcgtgagt tctgttaatt
    16621 gccgcgtaga tttgcctaat cgatgaagtt ggcaaatgac tagtgcatcg ccttgttgca
    16681 tgtcagcgat cactgactcc aaatgaacag cagtagaatc attttgttcc acttcatatg
    16741 tctcatggaa aatttcttga caaccaaagt cagataattg atcaacctga gttgttaaat
    16801 caacgtcatt tatggttgtg cgtgcataac caataatttt catggtgatt cctttctagc
    16861 agtgattttc taactggata taacagtaaa aatgtagcat aaagtacgtc aaaaagatag
    16921 ttttttcgca aattttccgc tgtctttttt tattggatga tcaagcagtt tttctttatt
    16981 tacgacggat tgcgtcaaga atctcaacgc ctttttctga gccgacaact actttagaaa
    17041 ccatgtttag gaaaagccca tgttctacta ccccaacaag ttcatctaaa taggtagcta
    17101 agctttctgg ctgttcgatg acttctagat gcagatcaat gatgtaatga ccaccatctg
    17161 tggttaagac ttctccgttt tcgtctgtac gcaagacggg ttggaatctt ttttcttcaa
    17221 aaatatgaag tagctgttgg gagccataag ggatcacttc aactgggagt gggaacttcc
    17281 caagtttatc gactaattta gaagaatcaa cgatccaaat cgtttctttt gagtacgtag
    17341 cgactatttt ctcgaaaagc aaagcagcgc cgccgccttt gatcccttga aaatcagcac
    17401 tgatttcatc agcaccgtct attgtcaaat cgactacggg gacttcatca atactcttta
    17461 acggtattcc taatttttcc gcttgttctt ttgtcgcatt tgaagtagta actcctgtga
    17521 tacgtaaacc ttcttcacgc atacggcgac caatctcttc taccatataa taggcggttg
    17581 aaccggtccc gagacctacg atcatcccgt cctttacgaa tttcgcggct tcaatgccag
    17641 ctaattgttt taaattcatc tttgcgtcca ccttttttta gtttgagcat cgtgctttcc
    17701 tcactatttt agcgtgattt acggtaaaaa gataccattc ttttttattc caataaagct
    17761 ttttcaaaaa aaattgcatc tcttgttgac atgttttttc attcgtgata ttctaacaaa
    17821 ggtgctttat ctacaggttt ctgtgaagaa acgtgctgac tgtttatcaa agcatatttg
    17881 ataaggatgc agacattgca tttatttttt atcacaaaaa aagaaccacc tggatgtgtg
    17941 gaactagacg caaatcgtgg gaaggaggaa acaaggaatg cctacaatta accaattagt
    18001 acgtaaacct cgtaaatcaa aggtggaaaa atctaattca cctgctttga acaaaggata
    18061 taacagcttt aagaaaactc aaactaacgt aaactcacct caaaaacgtg gggtagcaac
    18121 tcgtgtgggt acaatgacac ctaaaaaacc gaactcagct ttacgtaaat atgcacgtgt
    18181 tcgtttgtca aacttaattg aagttactgc ttatatccca gggattggtc ataacttaca
    18241 agaacacagt gttgtattat tacgcggtgg tcgtgtaaaa gacttaccag gggtacgtta
    18301 ccatatcgtt cgcggtgctt tagatactgc tggtgttaac gaccgtaaac aaagccgttc
    18361 taaatacgga actaaaaaac ctaaagctta attttaatta ttaaacaaat gaaacttgct
    18421 tctactatat agaagtaatt actatgaaca atatttcgga aggaggagtt acggatgcca
    18481 cgtaaaggtc ctgttgcaaa acgtgatgtt ttaccagatc ctatttataa ctcaaaatta
    18541 gtaactcgct tgatcaaccg tgtaatggtt gacggaaaac gcgggattgc agctaacatt
    18601 atctataatg cctttgactt gatcaaagaa tctacaggta acgatccatt agaagtgttt
    18661 gaacaagcaa tgaaaaacgt tcaacctgtt cttgaagtta aagcacgtcg tgttgggggt
    18721 tcaaactacc aagtaccagt tgaagttcgc cctgaacgcc gcgttactct aggtcttcgt
    18781 tgggtagtaa actacgctcg cttgcgcggt gaacacacaa tggaagaacg cttagcaaaa
    18841 gaaatcatgg atgctgcaaa caacacaggt gcttctgtta aaaaacgtga agacactcat
    18901 aaaatggctg atgccaaccg tgcgtttgct cactatcgct ggtaagattc tctctgtttg
    18961 gtcggctttt ataagtcgac ctcagatagt gtccttattt ttagcaacta gatagtaaag
    19021 agaggagaaa caaggagaat gaccagagaa ttttctttac aaaacactcg taatatcggt
    19081 atcatggccc acatcgatgc tggtaaaacg actgctactg aacgtatctt gtactatact
    19141 ggtaaaatcc ataaaattgg tgaaactcat gaaggagctt cacaaatgga ctggatggaa
    19201 caagaacaag aacgtggtat tacgatcact tctgctgcta ctacagcaca atggaagggt
    19261 caccgaatca acatcatcga cacaccagga cacgtagact tcacagttga agttgaacgt
    19321 tcattgcgtg tacttgatgg tgctgtaact gttcttgatg cacaatcagg tgttgaacct
    19381 caaactgaaa cagtttggcg tcaagcaaca acttatggtg tacctcgtat cgtatttgca
    19441 aacaaaatgg acaagatcgg tgctgacttc ttatattcag tatcaacttt acatgaccgt
    19501 ttgcaagcaa acgctcaccc aatccaatta ccaattggtg cggaagacga cttcacagga
    19561 attatcgacc ttgtaacaat gaaggctgaa atgtatacaa acgatcttgg aacagagatc
    19621 gaagaaacag aaattcctga agaataccgc gaattagctg aagaatggcg cgaaaaattg
    19681 gttgaagctg tagctgaaac tgatgaagaa cttactttga aatacttaga aggtgaagaa
    19741 atcactgaag ctgagttaaa agaaggtatc cgccgcgcaa cagttaacgt agaattctac
    19801 ccagtactat gtggttcagc atttaagaac aaaggggttc aattactatt ggacgctgtt
    19861 cttgattact tgccatcacc gttagatatc ccagctatca aagggattga ccctaaaact
    19921 gatgaagaag tagaacgtcc tgcagacgac agcgctcctt tctcagcttt agcatttaaa
    19981 gttatgactg acccattcgt aggtcgttta actttcttcc gtgtgtactc tggtgtttta
    20041 caatcaggtt catacgtaca aaacgcaaca aaaggcaaac gtgaacgtgt tggtcgtatc
    20101 ctacagatgc atgccaactc tcgttcagaa atctctgaag tttacgctgg agatatcgct
    20161 gctgctgttg gtttgaaaga cacaactact ggggacacac tatgtgatga aaagagtcta
    20221 gttattcttg aatccatgga attcccagaa ccagttatcc aagtcgctat tgaacctaaa
    20281 tcaaaagctg accaagataa aatgggtgtg gctttacaaa aactatctga agaagatcct
    20341 actttccgtg ctgaaacaaa cgtggaaaca ggcgaaacaa tcatcgctgg tatgggtgaa
    20401 cttcacttag atatcatcgt tgaccgtatg cgtcgtgaat tcaaagttga agctaacgta
    20461 ggtgcgcctc aagtatctta ccgtgaaact ttccgtgctg gcactcaagc cgaaggtaag
    20521 tttgtacgtc agtctggtgg taaaggtcaa tacggtcacg tatggattga atttacacca
    20581 aatgaagaag gtgctggttt cgaatttgaa aatgcgatcg tcggtggggt tgttcctcgt
    20641 gaatacattc cagctgttga aactggtttg aaagatgcta tggaaaatgg tgtcttagct
    20701 ggctatccat tagtagatat caaagctaaa ctttacgatg gttcatacca tgatgtcgat
    20761 tcaaatgaaa ctgccttccg tgttgctgct tctatggcat tacgtgcagc agctaagaaa
    20821 gctaatccag taatccttga accaatcatg gctgtagaag ttgttatccc tgaagattat
    20881 ttaggtgatg ttatgggtca cgtaactgct cgtcgtggac gtgttgaagg tatggaagct
    20941 cgcgctggcg gccaacaagt tgttcgtgcg atggttccac ttgctgaaat gtttggttat
    21001 gctacaactc ttcgttctgc tactcaaggt cgtggtacat ttacaatgac atttgaccac
    21061 tacgaagatg taccaaaatc agtacaagaa gaaatcatta agaaaaacgg cggaaaagct
    21121 gaataatttc ttaaaaccgg atagtcctgc aaagagatta tccaaagaga ctatattttt
    21181 aatatagttt cgtgtaaaat aattatcgat gatatgggca tgtggtgtac cttgtgtaca
    21241 tcatatacct atcagacaaa taaaaaatat attgtatttt taggaggaca ttttaaaatg
    21301 gcaaaagaaa aatttgaccg ttctaaacca cacgttaaca ttggtactat cggacacgtt
    21361 gaccatggta aaactacatt aacagctgca atcacaactg tactatctaa gaaaaacggc
    21421 ggccaagcta tggcttacga tcaaatcgat ggtgctccag aagaacgcga acgcggaatc
    21481 acaatctcaa cagctcacgt tgagtatgaa acagatacac gccactacgc tcacgttgac
    21541 tgcccaggac acgcggatta cgttaaaaac atgatcactg gtgctgctca aatggacgga
    21601 gctatcttgg tagtttctgc tgctgacggc ccaatgcctc aaactcgtga acacatccta
    21661 ttgtctcgtc aagttggtgt tccttacatc gttgtattct tgaacaaagt agacatggtt
    21721 gatgacgaag aattactaga attagttgaa atggaagttc gtgacctatt aacagaatac
    21781 gaattccctg gtgacgatgt tcctgtagtt gctggatcag ctttgaaagc tctagaaggc
    21841 gacgcttcat acgaagaaaa aattcttgaa ttaatggctg cagttgacga atacatccca
    21901 actccagaac gtgacaacga caaaccattc atgatgccag ttgaagacgt gttctcaatt
    21961 actggacgtg gtactgttgc tacaggtcgt gttgaacgtg gacaagttcg cgttggtgac
    22021 gaagttgaag ttgttggtat tgctgaagaa acttcaaaaa caacagttac tggtgttgaa
    22081 atgttccgta aattgttaga ctacgctgaa gctggagaca acattggtgc tttactacgt
    22141 ggtgttgcac gtgaagacat ccaacgtgga caagttttag ctaaaccagg tacaatcaca
    22201 cctcatacaa aattctctgc agaagtatac gtgttgacaa aagaagaagg tggacgtcat
    22261 actccattct tcactaacta ccgtccacaa ttctacttcc gtacaactga cgtaacaggt
    22321 gttgttgaat taccagaagg aactgaaatg gttatgcctg gcgacaacgt aacaatggaa
    22381 gttgaattaa tccacccaat cgctatcgaa aacggtacta aattctcaat ccgtgaaggc
    22441 ggacgtacag ttggtgccgg tgtcgttaca gaaatcaaag cttaattcac ttaagtttta
    22501 aagtaaagca acatttattc ggacgtaatc gctcggaacg aaggtcttag gatctttgtt
    22561 ccgagctttt ttatttattg caggatcagg aaaaaagaca ggagtattat aaatagaaga
    22621 aacacgaaat aaaagagtca gtattttcta aatgaaactt tatgaaaaca aaatgttttt
    22681 tacaagaaaa agccttgctt tgtgagttta tatatattat aatgacaaag gtgcgtttac
    22741 tttagtgaat tgcacagacg taatcagaat gatttcgtcg gctaagaaac gagaggttgc
    22801 gacacgcccg gacgctttgc cacgaacgag cgtgacggaa attttcgtgg agctatgtct
    22861 acttttcaaa ataggcgaag gagggaacaa gatggcaaaa caaaaaattc gtatccgttt
    22921 aaaagcgtat gaacaccgta ttttagatca atcagcggat aaaatcgtgg aaactgcaaa
    22981 aagaactgga gctagcgtgt ctggtccaat tccattacca actgaacgca gtctttacac
    23041 aatcatccgt gcgactcata aatacaaaga ttcacgcgaa caattcgaaa tgcgtacaca
    23101 caaacgtctt atcgacattg tgaacccaac accaaaaact gttgatgctt tgatgaagct
    23161 agacttacca tctggtgtaa acatcgaaat caaattataa aactaaaaca aaaataatgg
    23221 aggtgtactc atgaccaaag gaatcttagg gaaaaaagtg ggaatgacac aaatcttcac
    23281 tgaatctggt gaattaattc cagtaactgt tgtagaagct actccaaacg tagtactaca
    23341 agtgaaaaca atggaaacgg atggctacga agctattcaa gttggttacc aagatatgcg
    23401 tgaagtttta tcaaacaaac ctgcgaaagg tcatgttgca aaagcaaaca cggctcctaa
    23461 gcgcttcatt cgtgaattca aaaatgttga gctaggagaa tatgaagtag gaaaagaaat
    23521 tacagtagat gttttccaag caggagacat tattgatgta acaggtacta cgaaaggtaa
    23581 aggattccaa ggggttatca aacgccacgg acaaagccgc ggaccaatgg ctcacggttc
    23641 tcgttaccat cgtcgtcctg ggtcaatggg tccagttgca cctaaccgtg tatttaaaaa
    23701 caaacgtctt gctggccgta tgggtggcaa ccgcgtaact atccaaaact tggaagttgt
    23761 acgtgtcgat gcagaaagaa atgttatctt gatcaaaggt aatgttcctg gagctaaaaa
    23821 atctttaatt actatcaaat cagctgtgaa agctaaataa ttgtaggaag aaaggaggaa
    23881 ctaaggaatg ccgaatgtag cattattcaa acaagatgga agccaaaacg gtgaaatcac
    23941 acttaacgaa gaaatcttcg gaattgaacc taatgaaagt gttgtttacg atgcaatcgt
    24001 aatgcaacgt gcgtcattaa gacaaggaac acacgcggtt aaaaaccgta gcgctgttcg
    24061 cggcggcgga cgtaaaccat ggcgtcaaaa aggaacaggt cgtgcacgtc aaggatctat
    24121 ccgctcacca caatggcgtg gaggtggcgt agtctttgga ccaacaccac gttcttacag
    24181 ctacaaactt cctaaaaaag ttcgtcgttt agcaatgaaa tctgtattgt cagaaaaagt
    24241 tgctgaaaac aacttggtag caatcgaagg cttgaacttc gacgcaccaa aaacaaaaga
    24301 attcaaacaa gttcttgcta acttatctat cgactctaaa gtattggttg ttctagaaaa
    24361 aggtaacgac tttgctgctt tatcagcacg taacttacca aacgtttctg tagtaacttc
    24421 tgataatgtg agtgtgctag acgttgtatc aaatacaaaa gtgttagcaa cacaaactgc
    24481 tcttactcaa attgaggagg tgcttgcata atgaacttac tagacgtaat caaacgccca
    24541 gtgatcactg aaaaatcaat gcttgccatg gatgacaaga aatacacttt tgaagtggac
    24601 actcgcgcaa acaaaacttt agtaaaacaa gcggtagaag ctgcttttga cgttaaagtc
    24661 aaaaacgtga acatcgtgaa cgtacgtgcg aaattcaaac gtatgggcaa atatgcagga
    24721 tatacaaaaa aacgtcgcaa agcgatcgtt acattgactg aagattcaaa agaaatccaa
    24781 ttattcgaag ctgctgaata agcactaata gataactcat taaattagga gggaaaaacg
    24841 tggcgattaa aaagtacaaa cctacctcaa atggtcgtcg taacatgaca ggttctgatt
    24901 ttgctgaaat cacaacatca acacctgaaa aatcattgtt acaaccattg aaaaaccatg
    24961 ctggacgtaa caacaacggc cgcatcactg tacgtcatca aggtggcgga cacaaacgcc
    25021 aatatcgcgt gatcgacttc aaacgtaaca aagataacgt tgttgcaact gttcaaacga
    25081 tcgaatatga tccaaaccgt tcagctaaca tcgcgttagt acactatgaa gatggagtga
    25141 aagcttacat cttagcacct aaaggactag aagtgggtat gaaacttgtt tcaggtccag
    25201 aagcagatat caaagtcgga aatgctcttc cattagaaaa cattccagtt ggtactgtta
    25261 tccacaacat tgaaatgaaa cctggtaaag gtggacaatt gatccgttct gctggtacaa
    25321 gtgctcaagt acttggtaaa gaaggcaaat acgtattgat ccgcttgaac tctggtgaag
    25381 ttcgtatgat cttagctgca tgccgtgcta caatcggttc tgtaggtaac gaacaacacg
    25441 aattaatcaa catcggtaaa gctggacgct ctcgttggat gcgtaaacgc ccaactgtac
    25501 gtggtagcgt aatgaaccct aatgatcacc cacacggtgg tggtgaaggt aaagctccta
    25561 tcggacgtaa agcaccagtt tctccttggg gtcaaccagc tcttggttac aaaactcgta
    25621 acaaaaaagc taaatcagac aaacttattg ttcgtcgtcg caaaaataaa taattacttt
    25681 gaccatgtgt cgcacatttt gagaggaggt tcaccatggg tcgtagttta aagaaaggac
    25741 cttttgtcga tgagcattta atgaaaaaag ttgaagctca agcaggggtt gaaaagaaaa
    25801 aagttatcaa aacttggtca cgccgttcaa cagttttccc aagttttgtt ggatatacca
    25861 tcgcagttta tgatggacgt aaacatgtac cagtttacat ccaagaagac atggtaggac
    25921 acaaattagg tgaatttgca cctacaagaa cttatcgtgg acatgctgct gacgataaga
    25981 aaacgaaacg ctaatttgag gggaggataa atccaaatgg cagaacaaat cacatcagct
    26041 aaagcaactg caaaaacagt tcgcacttca cctcgaaaag cccgtttagt aattgacctt
    26101 atcagaggta aaagtgtcgc agatgcaatt tcaatcttga aattcacacc aaacaaatct
    26161 gctggaatca ttgaaaaagt attgatgtca gcagttgcga atgcagaaaa taactttgac
    26221 ttagatgttg aaaacttggt agtaagcgaa gcttttgtta acgaaggacc tacaatgaaa
    26281 cgtttccgtc ctcgtgcaaa aggatcagct tctccaatca acaaacgtac aagtcatatc
    26341 acggtagtcg tatcagaaaa ataaggaggg ataatcagtg ggtcaaaaag tacatccaat
    26401 tggaatgcgt gtaggcatca tccgcgactg ggatgctaaa tggtatgctg aaaaagagta
    26461 tgctgagttc ttacacgaag atttaagaat ccgtaaattt atttcaacta aacttgctga
    26521 tgctgctgta tcaacagttg aaatcgaacg cgcaacaggt cgcgtaaaca tttcaattca
    26581 cacagctaaa ccaggtatgg ttattggtaa aggcggttct gaagtcgaaa gcctaagaaa
    26641 agaattaaac aaattaactg gtaaaagaat ccatatcaat atcgtggaaa tcaaaaaacc
    26701 agatctagat gcaaaattag taggtgaagg aatcgcacgt caattagaaa accgtgttgc
    26761 tttccgtcgc gctcaaaaac aagccatcca acgctcaatg cgtgctggcg ctcaagggat
    26821 caaaactcaa gtatctggtc gtctaaacgg agcagatatc gctcgttcag aaggatactc
    26881 agaaggaact gttcctcttc atactttacg tgcggacatt gattacgcat gggaagaagc
    26941 agatacaaca tacggaaaac taggagttaa agtgtggatc tatcgtggag aaattcttcc
    27001 aactaaaaaa aacactgaga aaggagggaa ataatcatgt tagtacctaa acgtgtaaaa
    27061 caccgtcgtg aattccgcgg aaaaatgcgc ggggaagcta aaggcggaaa agaagtagca
    27121 ttcggtgaat acggtttgca agctgttgat tcacattgga tcacaaaccg ccaaatcgaa
    27181 gctgctcgta tcgcaatgac tcgttacatg aaacgtggtg ggaaagtatg gattaaaatt
    27241 ttccctcaca aatcttatac tgccaaagca attggggtac gtatgggttc tggtaaaggg
    27301 gcacctgaag gatgggttgc accagtaaaa cgtggtaaaa tcatgtttga aatcgcaggc
    27361 gttcctgaag aagtagctcg tgaagcgtta cgtctagctt ctcacaaatt accaatgaaa
    27421 actaagatcg taaaacgtga ggaaatgggt ggtgaatcga atgaaggtta aagaaatcag
    27481 agaattaacc actgccgaaa tgcttgatca agaaaaacaa ttaaaagaag aattgtttaa
    27541 tcttagattc caattagcaa caggtcaatt agaaaacact gcacgtatta aagaagtacg
    27601 taaatcgatt gcacgcatca aaactgtgtt gcgtgaacaa gctaagtaat tatggaagga
    27661 ggccattaag cgtatgactg aaggaagaaa tcaacgcaaa gtttatcaag gtcgcgtggt
    27721 ttcagacaaa atggataaaa caattaccgt tgctgtcgaa acaaagaaaa accaccctat
    27781 ctatggcaaa cgcatgaact attctaagaa atacaaagtg catgatgaaa acaacacagc
    27841 aaaagttggc gacatcgtga gaattatgga aactcgtcca ttatcagcta caaaacgttt
    27901 ccgtttgttg gaagtagtcg aagaagcagt tattatctaa taacacgaga ttttcctata
    27961 tagattgaat gcaaaatctg aaaggaggat acacaacgtg atccaagcag aaagtcgttt
    28021 aaaagtcgct gataactcag gcgcacgtga aattttgacg atcaaagtcc ttggtggatc
    28081 tggtcgtaaa actgctaaca tcggtgatgt gatcgttgct acggttaaac aagcaacgcc
    28141 aggtggggtt gtcaaaaaag gtgacgtcgt taaagccgtt atcgttcgaa caaaatcagg
    28201 agctcgtcgt acagacggtt catatattaa atttgatgaa aatgctgcag tgattattcg
    28261 tgatgataaa agccctcgtg gaacacgtat ctttgggcca gttgcacgtg aactacgtga
    28321 aaacaacttc atgaagatcg tttcactagc accagaagtt ttataatctt gagacactta
    28381 tcaaaggagg tgcgaaacag ttatgtttgt taaaaaaggc gataaagtta aagttatcac
    28441 tggaaaagac aaaaacaaag aaggcgttgt actagcagcg ttcccgaaaa aagataaagt
    28501 tattgtcgaa ggtgtaaatg tcgttaaaaa acaccaaaaa ccttctcaag ctgctcctca
    28561 aggtggaatt gttgagatgg aagcgccaat tcatgtttct aacgtgatgg tggttgactc
    28621 tactggtgta gctggccgag ttggctacaa agaagtagac ggcaaaaaag tccgtgtttc
    28681 taaaaaaacc ggtgaagttt tagataaata aaagtttaag gaaggagggt cttactgaat
    28741 gaaccgccta aaagaaaaat atcttaaaga agtaactcca tcattgatgg aaaaatttaa
    28801 ctatagctct gttatgcaaa cacctaaagt tgaaaaaatc gtcatcaaca tgggtgttgg
    28861 tgatgcggta tcaaacgcta aaaacttaga taaagcagtt gaagaattaa ctttgatcac
    28921 tggtcaaaaa ccaatgatta caaaagctaa aaaatcaatc gctggattcc gtttacgtga
    28981 aggaatgcca attggtgcaa aagttacatt gcgcggagaa agaatgtacg aattcttaga
    29041 caaattggtt tctgtttcac taccacgtgt acgtgacttc catggtgtaa gtaaaaaagc
    29101 ctttgacggt cgtggaaact atactttagg tattaaagaa caattgatct tcccagaagt
    29161 agattatgat ttagtagata aagtacgtgg tatggacatc gttattgtaa caacagcaaa
    29221 ctctgatgaa gaatctcgtg agttgttgac acaattaggc atgccattcc aaaaataatt
    29281 aaaggaggcg aactacgtgg ctaaaaaatc aatgattgct aaaaacaaac gtcctgcaaa
    29341 attttcaaca caagcttata ctcgttgcga acgttgcgga cgtccacatt cagtttatcg
    29401 taagttccat ctttgccgta tttgcttccg cgaacttgcc tataaaggtc aaattcccgg
    29461 cgtgaagaaa gctagctggt aaaaagtaaa ttcgcataag aaggaggtaa aagatcaatg
    29521 gtcatgacag atccaattgc agattttcta actcgtatcc gtaacgcaaa catggttaaa
    29581 catgagtctt tagaagtacc tgcatcaaaa attaaacgtg acatcgctga aatcttgaaa
    29641 cgtgaaggtt tcgttcgtga tgttgaatac atcgaagatg acaaacaagg cgtgatccgt
    29701 gttttcctaa aatttggtaa aaacggcgaa cgtgttatca caaacttaaa acgtatctct
    29761 aaaccaggtt tgcgtgctta tgtcaaagct gacgaagtac caaaagtatt gaacggttta
    29821 ggtatcgcaa tcatttcgac ttctgaaggt gttgtcactg ataaagaagc tcgtgaaaaa
    29881 aatatcggcg gcgaagttat cgcttacgta tggtaatcaa gtagaatcta caaggaggtg
    29941 tctttaagtg agccgtatcg gaaataaagt cgttgttatc cctgaaggag taacagttac
    30001 tcaagacgga aacaacgtta cagttaaagg accaaaaggt gaattgactc gtactttttc
    30061 agcagatatc aaaatgaata tcgaaggaaa cgaagtaact ttcactcgtc caaacgacag
    30121 taaagaaatg aaaacaattc atggaacaac tcgtgcgaac ttcaacaaca tggttgtagg
    30181 tgtcagcgaa ggtttccaaa aagcgttaga actaatcggg gttgggtacc gtgcacaatt
    30241 acaaggatca aaacttgtat tgaacgttgg ttactcacat ccagtagaaa tcacaccacc
    30301 agctggagta actgttgaag taccagctaa cacacaagta attgttaaag gtgcgaacaa
    30361 agaagaagtt ggcgaattag ctgcaaacat tcgtggaact cgtcctccag aaccttataa
    30421 aggtaaaggt attcgttatg ttggcgaata tgtacgccgt aaagaaggta aaactggtaa
    30481 ataatcgttt agaaaaattg catagaagcc agcagcagga aaaaggcatt tggctgaagt
    30541 ttaccttcaa aaaaatacat ttttacttgt gccgcagctt ctatttttcg aaacgtattt
    30601 actgcttgaa atagtagtac aataaaaata aagaggtgac aattgtgatt acaaaaccag
    30661 ataagaacaa aacacgtcaa aagagacatc gtcgtgtacg taacaatatc tctggtactg
    30721 ctgagcgccc acgcttgaac gtttttcgtt ctaacaaaaa catctacgct caaattattg
    30781 atgacgtagc gggtgtaacg ctagcaagtg cctctacctt ggataaagaa atttcaggtg
    30841 gaacaaaaac agaagcagca caagctgtcg gtaaactagt tgctgaacgt gcagctgaaa
    30901 aaggcattaa agtagtagtc tttgaccgtg gtggatacct ttaccatggc cgtgtgcaag
    30961 ctttagctga agctgctcgc gaaaatggac tagaatttta gaagaaggag gaataccatt
    31021 catggtttat atcgatccaa aacatttgga attagaagac cgcgttgttg caattaaccg
    31081 tgtaacaaaa gttgttaaag gtggacgtcg tctacgtttt gctgctttag ttgttgtcgg
    31141 tgacaaaaac ggacacgtag ggtttggtac aggtaaagct caagaagttc ctgaagcaat
    31201 ccgtaaagca attgaagatg cgaagaaaaa cttagttgaa gtacctatgg ttggttcaac
    31261 aatcccacac gaagtaatcg gtgcttttag tggtggtaga atcctaatga aacctgctgt
    31321 agaaggttct ggggttgctg ctggtggacc agttcgtgcc gtattagaat tagctggggt
    31381 agcagatatc acttctaaat ctttaggctc aaatacacca atcaacgttg ttcgcgcaac
    31441 agttgaaggt ttgaaacaat tgaaacgtgc agaagaagtt gcagaacttc gcggcaaatc
    31501 agttgaagaa ttaatcggat aaggaggaca acaataatgg ctgaattaaa agttacttta
    31561 aaacgcagta ttatcggacg tcctcaaaac caacgcgata cagttaaagc gttaggtctt
    31621 acaaagatta acagttcagt ggttaagcct tctaatgaag caattaaagg catgatcaat
    31681 actgtttctc acctagtgga cgttgaagaa atttaattta gaaaaatcat aactagaaca
    31741 ttaaggaggt gccaaaccta tgaaacttca tgaattaaaa cctgctgaag gttcacgcca
    31801 agtacgtaac cgtgttggtc gtggtacttc atctggtaac ggaaaaactg ccggacgcgg
    31861 acaaaaagga caaaaagctc gttcaggcgg cggtgtgcgt ttaggatttg aagggggaca
    31921 aacaccattg ttccgtcgtt taccaaaacg aggattcaca aacgtgaacc gtaaagacta
    31981 tgcagtcatt aatcttgatg tattgaaccg ctttgaagac ggagctgaag taactcctgt
    32041 tgctttagtt gaagctggaa tcgtgaaaaa cgaaaaagct ggaatcaaag tattagctaa
    32101 tggggaacta aacaagaaat taactgtgaa agcagcaaaa ttctctaaag cagctcaaga
    32161 agcaattgaa gcggctggtg gatcaatcga ggtgatctaa tgtttagact attaaaagat
    32221 gcttttaaag taaaagacat tagatcgaaa atcctattta ctgtatttgt gttgtttgta
    32281 tttcgcctag gtgcacatat tactgtacca ggcgtaaatg ctgcttcatt gcaaaacctt
    32341 tcttctttgc catttttcaa catgttgaac ttggtaagtg gtagtgcgat gcaaaacttt
    32401 tccattttct caatgggggt ttcaccatac attactgctt ctatcgtcat tcagcttctt
    32461 caaatggaca tcgtgcctcg atttgttgaa tggtcgaaac aaggggaagt tggtcgtaaa
    32521 aaactgaatc aagcgactcg atatctgaca ttagtattgg catttatcca atctgtcggt
    32581 atcactgctg gattcaatca atacgctcaa ttaggtttcg tgaataatcc taacgtagca
    32641 acttacgtaa cgatcggttt gattctaaca gccggtacga tgtttgttac ttggctagga
    32701 gaacagatca cagaaaaagg tatcggaaat ggcgtatcaa tgatcatttt tgcaggtatc
    32761 atttctagat tgcctgaaag tatcagagaa atttatgaag attactttat aaatattgat
    32821 ccttcagaca tatggaaatc tgctattttt gtggccattt tactagtagc ggtcttgttg
    32881 atcgtgacat tcgtaacatt tttccaacaa gctgaacgca agatcccgat ccaatatacg
    32941 aaacgcgtag ctggcgcgcc tacaagcagc tatctgccgt tgaaagtaaa tgcagctgga
    33001 gttattccag ttatctttgc tagttcgttt attgctacgc caaatgctgt tttacaagca
    33061 ttgagcggta gattcggttc tgaaaactgg tacaaagtga tgatggagat ctttaactat
    33121 aatacagcac ctggtgctac gatttatacg attctgatcg ttgccttcac tttcttctac
    33181 gcctttgttc aagtaaaccc tgagaaatta gcggaaaact tgcaaaagca aggaagttat
    33241 ataccaagcg tacgccctgg taaggggact gaagagtatg tctcaagatt actgatgcgt
    33301 ttaagtacag ttggtgcagt cttccttggt ttagtggcat tgcttccaat cattgctcaa
    33361 atggtttggg atcttccaca atcaatcgga ttaggcggta caagcttact gatcgttatc
    33421 ggtgtagctt tagaaactgc gaaacagtta gaaggcttaa tgctgaaacg taaatatgtt
    33481 ggattcattg agtaagaaag cgtgttgagg tttcccctca acacacgttc tcacacattt
    33541 attaggaggg aaaacaatga acctcatttt aatggggctg ccaggcgcag gtaaaggaac
    33601 acaagctgaa aaaatcattg atgcttatgg tattccgcac atctcaacag gagatatgtt
    33661 ccgcgcagcg atgcaaaacg aaacagctct tggcctggaa gcaaagtctt atatggataa
    33721 aggtgcatta gttcctgatg aagtaacaaa tggaatcgtg aaagagcgac tagctgaacc
    33781 agatacagag aaaggcttct tattagatgg ctttccacgt acgctagatc aagcaaaagc
    33841 tctggacgca atgctaaaag atttgaataa aaaaattgat gctgtcatcg atatccatgt
    33901 tggtgaagaa atcttggtcg aacgtctagc cggccgattt atctgcagca attgtggagc
    33961 aacttaccat aaaattttca accctacaaa agttgaagac acatgtgatc gttgcggtgg
    34021 gcatgaattc tatcaaagag aagatgataa acctgagacg gttaaaaatc gtctggctat
    34081 caacatcaaa aatagtgaac caatcttggc ttattataaa gaacaaggtt tgctaaacac
    34141 tattgatggt gctcgtgaaa tcgatgctgt atttgcagat gtacaaaaaa tcattgaaaa
    34201 atagcacgat tgcccgtagc cgacttgcat tatgcagtta ctcttgtcaa gttcaataaa
    34261 ttatgataga atatgtcagt ccgacttcta gagaatcctc tagatgatcc attttgagaa
    34321 ttttgaggtg ggaactgcag tttccgccat tttatcgtgt gaaaatgcga aacaagtaca
    34381 aggaggtact gtgcgtggca aaagaagata tgattgaagt cgaaggtaca gtcgtcgaaa
    34441 ctttgccgaa tgcaatgttt aaagttgaac tagaaaacgg acaccaagtt cttgctactg
    34501 tttcaggtaa aattcgtatg cattacatcc gtatcttacc tggtgacaaa gtaacggttg
    34561 aattgtctcc atacgatctt aaccgtggcc gtattactta tcgctttaaa taattgtact
    34621 ccgtaaaact acaggaggta ttatcatgaa agtaagacca tcagtaaaac caatgtgcga
    34681 acattgtaaa gtaattcgtc gtaaaggacg cgttatggtg atttgcccag caaatccaaa
    34741 acataaacaa cgtcaaggat aattggaggt gctataaata tggctcgtat tgcaggagta
    34801 gatattcctc gtgataaacg tgtagtagtt tcacttactt atatctatgg tattggtaac
    34861 actactgcta aaaagatctt agctgatgct ggtgtatctg aagatgttcg cgttcgtgaa
    34921 ttaacgaatg aacaaacaga tgctatccgt gcagaagtag ataaattaaa agttgaaggg
    34981 gatcttcgtc gtgaagtgaa cttgaacatc aaacgtttga tggaaatcgg ttcttaccgt
    35041 ggaatccgtc atcgccgtgg attgcctgtt cgtggacaaa acacgaaaaa caatgcacgt
    35101 actcgtaaag gcccagctcg cgctatcgct ggcaagaaaa aataattctt aagtgaagga
    35161 ggttaaaact tcatggcagc aaaaaaagtg aatcgtaaac gccgtgtgaa gaagaatata
    35221 gaagcaggta ttgcacatat ccattctaca ttcaataaca ctatcatcat gatcactgat
    35281 actcacggaa atgcattagc gtggtcatca gctggttcat taggtttcaa aggaagcaaa
    35341 aaatcaactc cttttgccgc tcaaatggca gcagaaactg ctacaaaagc agcaatggaa
    35401 cacggattaa agactgttga agtaacagtt aaaggacctg gttctggacg tgaagcagca
    35461 attcgttcat tacaagctac aggtttagaa gtgactgcaa ttcgtgacgt gactccagtt
    35521 cctcataatg gtgcccgccc tccaaaacgc cgtcgtgttt aatgagtgcc gctcattttg
    35581 agttttatca gtaacgtcat tgaacaagac acttttcgtt ttgaaagggg taaagataag
    35641 aatgattgag ttcgaaaaac caagaatcgc aaaaattgat gaagaaaaag attatggcaa
    35701 gttcatcgtt gaacctcttg aaagagggta tgggactact ttagggaatt ccctacgtcg
    35761 tattttatta tcttctttgc caggtgctgc catcactaat attcaaatcg atggtgtact
    35821 gcatgaattc tctaccatca aaggtgttag agaagacgtc actcaaatca tcttgaacat
    35881 taaaggctta gcgctgaaaa tgtatggcca agaagaaaaa acccttgaaa tcgatattac
    35941 cggacctgct acagtaactg ctggcgatat tatcgttgat agtgatgtag aaattcttaa
    36001 caaagatatg tacatctgta gtgtctctga aggagctacg ttccatgctc gcttaacagt
    36061 gaaacctgga cgtggttatg ttcaagcaga tgaaaataaa aaagaagata tgccaattgg
    36121 cgttcttcca gtcgattcaa tctacacacc tgttcgtcgt gtgaactatc aagttgagaa
    36181 cacacgtgtt ggacaccgtg aagattttga taaattgacg atggagattt ggacagatgg
    36241 ttcaattgaa ccgttggaag caatgagttt gtctgcgaaa atcatgactg aacatttgga
    36301 tatttttgtg aacttaactg atgaagcaaa aaatgctgaa atcatggtcg aaaaagaaga
    36361 aacacaaaaa gaaaaaatgt tggaaatgac gatcgaagag ttagacttgt ctgttcgctc
    36421 ttacaattgt ttgaaacgtg caggaatcaa tactgtacaa gaacttacaa ataaatctga
    36481 gcctgaaatg atcaaagtcc gtaacttagg acgtaaatca ttagaagaag ttaaagcaaa
    36541 attgcacgac ttaggtttag gtttacgtaa agaagattaa aaccttttac gaaggaggga
    36601 aaccaacgtg agttatcgta aactaggacg cacatctagc caacgtaaag cgatgttgcg
    36661 tgatttaaca actgatttat taatcaacga acgtattgtg actactgaag ctcgtgcgaa
    36721 agaagttcgc tcaactgcag aaaaaatgat cactttaggt aaacgcggag atttgcatgc
    36781 acgtcgtcaa gctgcggctt tcgtacgtaa tgaagttgcc agcgtgcgtg aagaaaatga
    36841 ggacatcgtc attgaatcag ctttacaaaa actgttcaac gatattgcac ctcgttacgc
    36901 agaacgtcaa ggcggataca cacgcatttt gaagacagaa ccaagacgcg gtgatggtgc
    36961 accaatggtt gtcttagaac ttgtctaaaa tcacactaat aacatcactt tatagatgat
    37021 tcttttagag tgttatgatg ttggaagata tttttccgag tctagctcag cgctgctcct
    37081 caagcgaata tcgcttgggg agtaagcact catcataaag tgttttttta taccttcaat
    37141 tacctgtcat gacagatatg tttgaaggca taaaaaaaca ttttaagcga gaaataattg
    37201 aaaattttgt ctttttttga tagtgttagt aggaacttta ggatgaagaa ggagtaatca
    37261 tggagccaat tattgaatta gaaaagataa attataaata ccagccagat gatcttcgcc
    37321 ccgcattgaa agatgtctcc tttacaattg ataaaggtga gtggatcgct attattggac
    37381 acaatggttc aggaaaatcg acacttgcca aaacgatcaa tggattgctt ctgccagaat
    37441 cagggatcgt gaaagtggga aatcaaatat tggatgaaga aaatatttgg acgatccgac
    37501 agatggttgg aatgattttt caaaatccag acaatcagtt tgttggttcg acagtagaag
    37561 atgacgttgc ttttggtctt gaaaatcaag ggattcccag agaagaaatg ctagtgcgtg
    37621 tgaaagatgc acttgagaaa gttagaatgg ctgagtttgc ttcacgcgag cctgctcgtt
    37681 tatcaggagg gcaaaaacaa cgtgttgcca ttgccggcgt ggttgcctta cgacctgata
    37741 tcattatttt agatgaagca accagtatgc tagatccaga aggtcgtgaa gaagtgatct
    37801 ctacgatcaa gaagatcaaa gaagaaagtc aattgaccgt tatatctatc actcatgata
    37861 ttgatgaagc agctaatgcc aatcggatac tagtgatgag acaaggcgaa cttgtccgtg
    37921 aaggtacacc aaaagaaatt ttttctgctg gtcctgaact gatcgactta ggtctagatc
    37981 taccatttcc tgaaaaactg aagagcgcac tgaaagaacg tggggttgat gtgcccagtg
    38041 aatatatgac agaagaaagg atggtggatt ggttatggac atccgttttg aacaagtaga
    38101 cttcacttat cagccgaata caccatttga acaaagagcc ttatttgaca tcaatatgac
    38161 gatcaaagaa aacagttata ctgcattagt agggcatacc ggaagcggga aatcaacctt
    38221 acttcagcat ttgaatgcgc ttgttaagcc aacaagcggt acagtccata ttggagaacg
    38281 agatattcag ccggatacgg ataataagaa tttaaagccc atccgaaaaa aagttggcat
    38341 cgtctttcag tttccagaag cacagctatt cgaagaaacg gtagcaaaag acattgcttt
    38401 cggtcctaaa aactttggtg tcagcgaaga agaagcacta gtcctagcaa aagaaacatt
    38461 agaacaagtt gggctggatg aaagctattt ggaacgttcg ccatttgaac tttcaggagg
    38521 gcaaatgcgt agagtagcaa tcgctggtgt gcttgccatg aggccggaag tacttgtatt
    38581 ggatgaacca acggcaggac ttgatcccca gggcagaaaa gaaatgatgg agatgttttg
    38641 gcgtcttcat aaggaacagc agatcactat tgtattagtt acgcatttaa tggatgatgt
    38701 agccaatttt gcagattatg tctatgtgtt ggaaaaggga cgaatcgtta atagcgggga
    38761 gccgcaagag gtctttcaaa acattgaatg gctaaaagaa aaacaactag gcgtacctac
    38821 tgccaccgag tttgcagaag aattaatggc taaagggatg aactttgcta ccttgccttt
    38881 gacagctgaa gaattagcag acgctatcgt ccattatgct ggaggaaatg cccatgatga
    38941 ataaattgat atttggtcgt tatatgcctg gagattcctt gatccatcga ttggatcccc
    39001 gagctaaatt attagccagt ttttatttta tcggaatcat ttttttagca aataattggg
    39061 aaacgtacct tttgttggca tttttcacat tactttctgt cttcttgtca aaagtagatt
    39121 tgaaattttt tatccgaggc attcgcccat tgatctggct aatcctcttc actgtagctt
    39181 tgcaggtctt gtttacaagt ggcggagaaa cttattggag ttggggtgtt ttccatatca
    39241 ctgaatttgg tgtgatgaac ggcctcttta ttttctgtcg attcgtgttg atcattttta
    39301 tgtcgactct actcacgctt actacaccgc ctttggaatt atcggatgcg attgagtact
    39361 tgcttcgtcc attgaaagcc gtacggttcc cagtccacga aatttcattg atgctgtcga
    39421 ttgctcttcg ttttgtgcct acattgatgg acgaaacaga aaagatcatg aacgctcagc
    39481 gcgctcgcgg tgttgatttt ggtgaaggaa acctagttca gaaaatgaaa gcagtcattc
    39541 ctttgctgat tcctttattt gtcagcagct tcaaccgtgc ggaagatctt gcgactgcta
    39601 tggaagcaag aggctacaaa ggtggggaag gaagaacgaa atatcgtgta ttgcattggc
    39661 ataatagaga cacgatggtt atcattgctt ttgtattatt aactgtgcta ctgatctttt
    39721 taagaggata aacgttcagt gggccgctat ctattctttt cttgctgatc tgtttgcaag
    39781 aaaggatgca agcagtgtat gacaagaaat attagggtct gtggcagact aaggtcacag
    39841 gcctctttct tttatctgaa aagaaagaac gaaaaaattt tatagaaaaa tcattattcc
    39901 agataacctt ttaggaacaa aggagagaaa aacatggtac gttacaaagc aatcattgcg
    39961 tacgacggaa caaattttaa tggattccaa aaacagccaa atgggcgtac tgttcaagaa
    40021 gaagtagaaa aaacattaca aaaaatggca aatggaaaag aaatcacggt ctttggttcg
    40081 ggaaggacag atgccggagt acatgcgatc gggcaagtaa tccattttga ttatccagag
    40141 gagcgtccgt tagaacgaat gcgtttcgcg ctagataccc agtcaccaga ggatattgct
    40201 gtaagacaag tagaaattgt ttcagaagac ttccacgcaa gatatttggt caaagaaaag
    40261 acgtatcaat ttcgagtgga tatagggaaa ccacgcagtc cttttcgacg ccattatgca
    40321 agttattttc cttacccact tgatttggaa aaaattcagc gggcgcttcc tgatttatta
    40381 gggactcacg atttcacctc tttttgtgca tcaggaagct cgatagagga taaaatacgt
    40441 acgatctacg aggctaagat ggaggtaaat gaaacaaaag acgaattgtt gtttacattt
    40501 aggggaaatg gcttcttgta taagatgata cggatcttag taggaacgct tttgaaaatt
    40561 ggcaatggcc gattgcctga agatagtatc ccagatatta ttgcgaaaaa agatcgtaat
    40621 gcagcaggac ctacagcaca ccctgaagga ctttatttgt atgaagtgaa atatgaataa
    40681 gaggttatga tagaagtggt cagcttcaag aaataagaag gtatttacga aaattacttc
    40741 tcaaaatttt gcaaaatttc ggcttatttt cgaagaagct acttctggaa caccgtttaa
    40801 gaaagaggtc gtgagcctgt catttaactc tgagtcacaa agagcaattt caaaaaacag
    40861 cttctcatct ttgatgaata tcgtgacttg tggacgaggc gttgataagt gaatcatctt
    40921 tttgttacct attttttctg taaccaaaaa acgatggaac gttgttttga ttgcccttgg
    40981 tgcggacagt ctgaacaggt tccatcgttt ctttattttc tacgtcttct tctgtgtgtg
    41041 tttcgtggtt gttcttctat tgctggatca atcgtgccat aaaaggggcg attgcggtca
    41101 aggtaccaga cataaagaat aaacaatgca atacatagga caaaaaggat tgcacctatc
    41161 gtaaatcctt caggcaagta actgaaagtg acagtatgtt tacctgctgg aacttttatc
    41221 atcaagaatg cgtctttgaa attgctgacc ttgactggtt tcccatcaat tttagctttc
    41281 catcctttgt cataaggaat agttgtcacc aacatggaat cttcagcggt atttacagtc
    41341 ccagaagctt ttctgccagt ggcttccatt tcgacacctt tttctttgat ctgattcatt
    41401 gcggcttgat aggctacaac atctagacca acgactttag gctccatgaa acttatttct
    41461 ttcgtcccat aaaaacttgc agtaaatgtc actgttgtct cttgaggata atagccaaga
    41521 tcatagtatt gaccagaaat gttgatttgc gtttttcgcg atgtaccatt gacagtgatc
    41581 gtcgcactag aactttctaa ttgcgcaaaa ttggttggga acaaacttaa gtaagcttgt
    41641 gtatttgcag gcacatggac ttcccaagtg agctcttttg gctggttatc tgctgtttct
    41701 gagtaagtga cgccagtcgt tgtctttgtg atcgtcgtat tcaactgatt cttcaaagta
    41761 ggtgtataga atgtaaaata ctgttcattt agaccagaca atgtattgat caagtttgtc
    41821 tgactggtca aattatctgt cactggctgc tcaacatcat taacggacaa cggtgcagta
    41881 aatgctagtg gcaatgcatt gacattctga tacagtgtat agtcgccgct ttgaccagct
    41941 tggcggaagc catatttact gaacgtgctt tcgtttttag aaatgttata tttgattcct
    42001 gtaaacccat ccatcagcaa agtattattg ttataacgga tattcaaatt tgtccctctt
    42061 gaacggaaac ctaatttatc caggtaagag gaagaattac gatttcttat agaagaaaac
    42121 aaactgatac cactgtaacc atagttgatc gaatcgtttg cagatactgg atccagattt
    42181 tccaagcggt aaaacgagtc ggaatctttt tttgtttcat ttacaagagc tttcaagtca
    42241 ggataaggat cagtatataa actgcgggaa gcataattcc aatcatcaag aatgccatgg
    42301 atcatactgt tcgtattgac cagtgcttca gcagacatga tcaataataa caatatactc
    42361 agatgattga atgaaagttt tttatgctga taaaatccca cagcaaaagc ataaatccct
    42421 aagaaaacca ctgtcaatac aaaggaagtc atcgtcacat aggtgtagct tccttcaggt
    42481 ttgatgcccc atgccaccgc aaagagagca gctacgacca aaatgattcc taacaacagc
    42541 ccaccatcgt cttgttctat cttttcccat ccttttcctg caagcaggag cactaagaaa
    42601 gagaacagat aggcataacg gaataaaaac atattcggtg catgcatacc atgccagaac
    42661 aaattcaatg gaacaagata aaaacttgcg ataagtatgg cgaaaagact gccgtacaaa
    42721 aatttatttt tcaacggtac tttccgactg gcaaaataaa acagacagag gactaatggt
    42781 agcagacctg cgtaaacgaa cggaatcgat ccgtattttg tcgtgtcata aacaccaatc
    42841 atatttttca tgattagatc cagataagaa gtcgcttctg ttttcaacgt agtgatctga
    42901 ctcagtgcct cgccatttga ccgaagatcc agcactgctg gcaatacaat gatcattgaa
    42961 gcgccgcctg ccagtaaaga agtgatacca taaggaacaa tcgttttttt atattctgac
    43021 cagtttgtca atagacgggc gataaaatat aagaaagaaa atacgccaat cataaaaccc
    43081 atataaaaac tggataaaaa caagagtaaa taactgataa ataaaagttt cggccgttta
    43141 agatccataa cacgatgaat ccccaaaatc actaatggaa gataaacaaa tgcatccagc
    43201 cacatgatga tctccgaatg tgcagtgata aatgagatca atgcgtaact gacgctaagt
    43261 gcaacatgcc cccagcgagg aatcttgaac gtacgtgcaa gaaaccaaaa agccaatccg
    43321 gcacagccga ccttaagcaa agtcagaaca tacagtgcat ctggcatcat ctggtttgga
    43381 aaaaagaaca ccagtggcgt gaagatcccg cctagatagt aagaaattaa agataaataa
    43441 ttcaatccta gagaagcatt ccatgtataa aaaatacttt gctttccaag aagaacattt
    43501 cggaagctgg catgaaaatt agaaaactgt gaaaaggcat cacttgccaa aatacttctg
    43561 ctgcttccag gatagattcc gatacttaga taaacaagtg ctaaaatcaa aaacggaatc
    43621 aggaaactag ctgctgcgta gcgtccgttt tctctaaaga atgactttaa attttttttc
    43681 atatttattt tccaccaaat ttctatgaag ttgtattcag tttatcatat cct
//
LOCUS       DARBFD010000012        43368 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000012 DARBFD010000000
VERSION     DARBFD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.012"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(8..2389)
                     /locus_tag="SCK08_000667"
     CDS             complement(8..2389)
                     /locus_tag="SCK08_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1200580.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="SCK08_000668"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="SCK08_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEG1200581.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="SCK08_000669"
     CDS             3090..3629
                     /locus_tag="SCK08_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEG1200582.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="SCK08_000670"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="SCK08_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEG1200583.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="SCK08_000671"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="SCK08_000671"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="SCK08_000672"
     CDS             4736..5902
                     /locus_tag="SCK08_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEG1200584.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="SCK08_000673"
     CDS             5948..7444
                     /locus_tag="SCK08_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEG1200585.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="SCK08_000674"
     CDS             7525..8208
                     /locus_tag="SCK08_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1200586.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8853..10043
                     /locus_tag="SCK08_000675"
     CDS             8853..10043
                     /locus_tag="SCK08_000675"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEG1200587.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10145..11098
                     /locus_tag="SCK08_000676"
     CDS             10145..11098
                     /locus_tag="SCK08_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEG1200588.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11167..12516
                     /locus_tag="SCK08_000677"
     CDS             11167..12516
                     /locus_tag="SCK08_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1200589.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="SCK08_000678"
     CDS             complement(12699..13022)
                     /locus_tag="SCK08_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200590.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14314
                     /locus_tag="SCK08_000679"
     CDS             13151..14314
                     /locus_tag="SCK08_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1200591.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14384..15733
                     /locus_tag="SCK08_000680"
     CDS             14384..15733
                     /locus_tag="SCK08_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1200592.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15778..16209)
                     /locus_tag="SCK08_000681"
     CDS             complement(15778..16209)
                     /locus_tag="SCK08_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1200593.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16597..19239
                     /gene="alaS"
                     /locus_tag="SCK08_000682"
     CDS             16597..19239
                     /gene="alaS"
                     /locus_tag="SCK08_000682"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEG1200594.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19439..20035
                     /locus_tag="SCK08_000683"
     CDS             19439..20035
                     /locus_tag="SCK08_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1200595.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20157..21305
                     /locus_tag="SCK08_000684"
     CDS             20157..21305
                     /locus_tag="SCK08_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1200596.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21343..21756)
                     /locus_tag="SCK08_000685"
     CDS             complement(21343..21756)
                     /locus_tag="SCK08_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEG1200597.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21835..22266
                     /locus_tag="SCK08_000686"
     CDS             21835..22266
                     /locus_tag="SCK08_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEG1200598.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22365..22598)
                     /locus_tag="SCK08_000687"
     CDS             complement(22365..22598)
                     /locus_tag="SCK08_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEG1200599.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22781..24451
                     /locus_tag="SCK08_000688"
     CDS             22781..24451
                     /locus_tag="SCK08_000688"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEG1200600.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24598..24984
                     /locus_tag="SCK08_000689"
     CDS             24598..24984
                     /locus_tag="SCK08_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200601.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            24971..25669
                     /locus_tag="SCK08_000690"
     CDS             24971..25669
                     /locus_tag="SCK08_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1200602.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25674..26138
                     /locus_tag="SCK08_000691"
     CDS             25674..26138
                     /locus_tag="SCK08_000691"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEG1200603.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26135..27046
                     /locus_tag="SCK08_000692"
     CDS             26135..27046
                     /locus_tag="SCK08_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1200604.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27281..27820
                     /gene="pyrR"
                     /locus_tag="SCK08_000693"
     CDS             27281..27820
                     /gene="pyrR"
                     /locus_tag="SCK08_000693"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEG1200605.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27834..29117
                     /locus_tag="SCK08_000694"
     CDS             27834..29117
                     /locus_tag="SCK08_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEG1200606.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29130..30056
                     /locus_tag="SCK08_000695"
     CDS             29130..30056
                     /locus_tag="SCK08_000695"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEG1200607.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30075..31355
                     /locus_tag="SCK08_000696"
     CDS             30075..31355
                     /locus_tag="SCK08_000696"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEG1200608.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31360..32436
                     /gene="carA"
                     /locus_tag="SCK08_000697"
     CDS             31360..32436
                     /gene="carA"
                     /locus_tag="SCK08_000697"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEG1200609.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32441..35626
                     /gene="carB"
                     /locus_tag="SCK08_000698"
     CDS             32441..35626
                     /gene="carB"
                     /locus_tag="SCK08_000698"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEG1200610.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35680..36456
                     /locus_tag="SCK08_000699"
     CDS             35680..36456
                     /locus_tag="SCK08_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEG1200611.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36453..37385
                     /locus_tag="SCK08_000700"
     CDS             36453..37385
                     /locus_tag="SCK08_000700"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEG1200612.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37382..38098
                     /gene="pyrF"
                     /locus_tag="SCK08_000701"
     CDS             37382..38098
                     /gene="pyrF"
                     /locus_tag="SCK08_000701"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEG1200613.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38105..38794
                     /locus_tag="SCK08_000702"
     CDS             38105..38794
                     /locus_tag="SCK08_000702"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEG1200614.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            38767..39036
                     /locus_tag="SCK08_000703"
     CDS             38767..39036
                     /locus_tag="SCK08_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200615.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39074..39715
                     /locus_tag="SCK08_000704"
     CDS             39074..39715
                     /locus_tag="SCK08_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1200616.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39758..40450
                     /locus_tag="SCK08_000705"
     CDS             39758..40450
                     /locus_tag="SCK08_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEG1200617.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40447..41349
                     /locus_tag="SCK08_000706"
     CDS             40447..41349
                     /locus_tag="SCK08_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1200618.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="SCK08_000707"
     CDS             complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="SCK08_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEG1200619.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13874 a         7663 c         9440 g        12391 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaagc
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8281 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8521 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     8881 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     8941 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9001 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9061 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9121 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9181 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9241 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9301 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9361 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9421 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9481 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9541 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9601 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9661 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9721 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
     9781 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
     9841 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
     9901 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
     9961 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10021 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10081 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10141 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10201 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10261 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10321 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10381 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10441 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10501 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10561 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10621 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10681 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10741 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    10801 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    10861 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    10921 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    10981 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11041 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11101 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11161 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11221 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11281 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11341 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11401 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11461 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11521 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11581 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11641 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11701 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    11761 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    11821 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    11881 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    11941 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12001 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12061 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12121 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12181 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12241 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12301 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12361 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12421 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12481 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12541 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14281 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14341 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14401 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14461 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14521 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14581 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14641 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14701 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    14761 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    14821 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    14881 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    14941 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15001 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15061 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15121 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15181 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15241 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15301 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15361 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15421 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15481 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15541 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15601 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15661 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15721 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    15781 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    15841 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    15901 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    15961 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16021 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16081 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16141 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16201 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16261 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16321 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16381 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16441 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16501 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16561 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16621 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16681 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16741 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    16801 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    16861 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    16921 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    16981 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17041 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17101 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17161 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17221 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17281 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca
    17341 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17401 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17461 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17521 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17581 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17641 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17701 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    17761 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    17821 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    17881 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    17941 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18001 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18061 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18121 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18181 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18241 ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat caaaaaccat
    18301 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18361 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18421 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18481 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18541 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18601 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18661 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18721 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    18781 caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    18841 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    18901 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    18961 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19021 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19081 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19141 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19201 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19261 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19321 cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19381 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19441 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19501 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19561 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19621 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19681 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19741 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    19801 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    19861 attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc ttttatttaa
    19921 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    19981 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20041 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20101 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20161 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20221 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20281 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20341 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20401 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20461 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20521 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20581 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20641 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20701 aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca ttatatatcc
    20761 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    20821 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    20881 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    20941 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21001 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21061 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21121 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21181 tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta cgtagtcaaa
    21241 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21301 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21361 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21421 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21481 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21541 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21601 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21661 tagctgttct tgtttacctt gatagacaat caagattcca cctccactag gaccagggtt
    21721 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    21781 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    21841 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    21901 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    21961 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg ttcttttatt
    22021 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22081 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22141 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22201 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22261 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22321 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22381 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22441 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22501 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22561 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22621 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22681 gtaattttat gttttttttc tgaataaaaa aaggtataat tggcatatct taacaaagaa
    22741 gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa atgaagaatg atctgcaaat
    22801 ttcacaagaa acagagctag aaccgattgt tgaagtggct agtaaattag atcttaagga
    22861 agacgatttg gaactgtatg ggaaatataa ggcgaaaatc aatttttctg gaatcaatcg
    22921 atcaaaggat aacgctgagg ggcatttgat cttagttact tccatcaatc caacaccagc
    22981 tggcgaagga aagtcaacga tcactgtagg gttaggagac gcgctcaata aaatcaataa
    23041 aaaatctgtt attgcattgc gagaaccctc acttggtcct gtgatgggaa tcaaaggtgg
    23101 tgctgcaggc ggaggatatg cacaggtatt gcctatggaa gatatcaact tgcattttac
    23161 tggtgacatg catgctatca caacagctaa taatgcatta tctgctcttt tagataatca
    23221 tattcatcaa ggaaatgaat tgaacatcga tgcccgccga gtgatttgga aacgggtagt
    23281 cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc ttgggtgggc caatccaagg
    23341 tgttccaaga gaagatgggt ttgatatcac agttgccagc gaaatcatgg ctatcttgtg
    23401 tcttgcggca gaccttgaag atttaaaggc tcgtcttgca aggatcgttg tcggctatac
    23461 atttgaccgt cagcctgtga ctgctggaga cttgaaagca gaaggcgctc tggcattact
    23521 gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt tacggaacac cagcttttgt
    23581 ccacggggga ccgtttgcca atatcgctca tggctgcaat agtattttag ctaccaagac
    23641 agcattgaga ttagcagatt acgttgtcac agaggcagga tttggtgcag atcttggcgg
    23701 agagaaattt ttagatatca aagtacctaa cttgaaaaaa gcgccggatg ctgtggtgat
    23761 tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg aaaaaagatg aacttaaaaa
    23821 tgagaatctt gatgctttga aagtaggttt tgcaaattta aagcgccata tacgtaatat
    23881 ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa ttcgtaacag atacagacaa
    23941 tgaactcaca ctgttggagc acttgtgtga aaatcagggg attcttgcca agagagcgag
    24001 tgtatgggca gatggagcag aaggcggcgt tgatttagca aaagctgtag tacgattgat
    24061 tgatagaaaa gaagctaatt acaagccatt ataccgccta gaagaaacga tacaagaaaa
    24121 gacagaaaca atcgtcaaga aaatttacgg aggaaatggt gttgttttca gcaaaaaagc
    24181 gcaaaaacaa atagaagagt ttacgaagaa tggatgggac aaactgccga tttgtatggc
    24241 aaaaacacag tattcatttt ctgatgatcc ttcactgcta ggtgcaccgg aagatttcac
    24301 catcacgatc cgcgaaatca tcccaaaatt aggggcagga tttttagtag ccttgactgg
    24361 agatgttatg acgatgcctg gtttgcctaa aaaacctgct gcattgaata tggacgtgac
    24421 aaatgacgga gaagtgttgg gattgtttta agcttgatta taatctacca tcgaaggagc
    24481 tgtattttga ctacagcttc tttttttaga cgatagggaa ttaattttgt ttacactagg
    24541 atcattcttt ttctttattg tattttgagc tatactaaag cataatagga gggtatgatg
    24601 aaaacgacat ttataagaaa aaccggtttt tacggtatgg gtagctcgat tcagctaaga
    24661 atcaacaatg aaaaacgcag tgttctcagt catgaacaaa agataacgct tgatctggaa
    24721 gtgcccttta cgatgcaggt caccttttat tggctaaaaa gccccgttta tacagtgaaa
    24781 gaagcaaagc ctgcttatgt gatcacaatg aattacttga ttcttcaaat ctatccgttt
    24841 ttatttctag caactgggtt gggggccgtt ttcctacaaa atttattttt cagttttttt
    24901 gcagttattg tgttgattgc gttctttttc tatataaaaa accgggcata tttgatcaag
    24961 gaggaaaatt atggcaaact ctgatctttt tttaatcagt ttcaatcgaa tcgaaaaatg
    25021 gatgagagac gagatgggaa atgcacgaaa tatgggattt accgaacttg tcagacgcct
    25081 agctcaaaga aaacaattaa tgatcagaaa atacgaagat gatcttttac agcttgctca
    25141 gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat tttgtcattg cagagccaaa
    25201 tgattggaca gtcaagcgaa tccagctgat cgaacaagaa ctgattcagc ctgaaaccgt
    25261 gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa gatcttccgt tacttgaact
    25321 gttaaagata gtagcagaaa aaaggtattc acaatttccg ttatacaata aaggaaaatt
    25381 tgtagcattg attactttac gtaatatcgg gttttggcta gcaaaagaaa gccaaaaagg
    25441 tccggttgat ttaacaaata aacgagcgtt ggatttgatc atccaaaacg gaaagtacac
    25501 aaattatcat tttgttccag ctaaaacaca tatttacgag gttgaagcga tgtttcgtga
    25561 gcaagggact ttggaagccg tacttatcac taaagatggg aatcccgatg gtaatctgct
    25621 cgggatcgta cgacctcgtg atatttataa acaaatagaa aaggattgat tttttgttaa
    25681 ttatttattg gatattcagc gctttgctag taggagtgga tcagttggtc aaatggtgga
    25741 tcactgataa tctagcgctt ggagagacga aaaatcttat accaggtatt ttgtcactga
    25801 atcatattcg aaacactgga gccgcatgga gtatgctaga agggaagatg tggttcttta
    25861 ctatagtgac actcattgca gtcgttgtga ttctcacatt gatgattaaa aatcgtgaaa
    25921 aaggtaatcg ctggttcatg attggattga gtttgattct agctggtgcg attggcaatt
    25981 ttatcgaccg tattcgtctt ggctatgtag tagatatgtt ccagacagat tttatgagct
    26041 ttccgatttt taacgtagca gatgtgacac ttgtatgtgg agtgatttgt atgttaattt
    26101 atattatttt agatgaaaag gatcaaagaa aaaaatgaca gaacttaaaa cgacgattca
    26161 ccatgaatct ggtaggatcg ataaagtatt gactaattta ttcaccgatt acagtcgttc
    26221 gcaaattcag ctgtggctga aaaatggagc ggtttctgtc aatggacagc cagtcaaagc
    26281 aaattacaaa gtaaaacaag gagacgagat tactgtcttg atccccgaac cagaaacatt
    26341 agatgtggtc ccagaaaata tcccgttgac gatcgtttac gaagatgaag atgtagcggt
    26401 gataaacaaa cctcaaggaa tggtcgttca tccttcagca ggtcatacgc atggcacaat
    26461 ggtcaatgcg ctgatgtacc atatcaagga tctttctacg atcaacggcg ttattcgtcc
    26521 aggtattgtt caccgaatcg acaaagatac ttccggactg ctaatggttg ccaaaaatga
    26581 ccaggcacac gaatcattag ctcagcaatt aaaagataag acctctttgc gcaaatatgt
    26641 tgctttagtc catggagaga ttcctcacga aaaaggaacc atcaatgcgc caatcggccg
    26701 ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat gggaagtcgg ctgtcacaca
    26761 ttttactgta ttggaacgtt tcgaaggatt tactctggtt gaattaaaac tagaaaccgg
    26821 aagaacacac cagatccgtg tgcatatgaa gtatatcggt tatccgttag ctggagatcc
    26881 agtatacgga ccgaagaaaa ctttgaaagg caatggtcag tttctccatg cgcaaatgtt
    26941 aggttttacg cacccgacta caaacgaaaa gatgatcttt gaagcacctc ttccagaagt
    27001 ttttgaaaaa actttagaaa aattaagaaa aaacattgca ttttaattta gataacggta
    27061 tagtaattaa caacgaaagc ataaaactta atagaattcc tttaagttta gtcccgtgag
    27121 gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat aagtagattg ctcattgaca
    27181 tagtatagct atcagtgtat cagtatacgc tcagtctaaa cattcctcct gtcctcttag
    27241 ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa atgcaagcaa aagaagtagt
    27301 agatcaggta accatgaagc gagcattgac tcgaattact tatgagatta tcgagagaaa
    27361 ccatagtatc caagatatcg tattggtagg tatcaaaaca cgaggaatct acattgcatc
    27421 aagaattgcc gaacgcctga agcagctaga agatatcgat attcctgtag gagaactgga
    27481 tatcacttta taccgagatg acaaaaaaga aaatccagaa gaaccggaac ttcattcttc
    27541 tgacatccca gtttccttag aaggaaaaga agttattttg attgatgatg ttttgtatac
    27601 aggacgaacg attcgagcag caatggacgc agtaatggat tttggcagac caagaaaaat
    27661 ttcattagca gtcctggtcg atcgtgggca tcgagaattg ccaatccgtg cagattatgt
    27721 tgggaagaac attccaacat ctcgggcgga agagatttta gtagagatgc aagagcttga
    27781 tggacaagat cgcatcatga ttttgaaaga ggaagattag aggagagaga aacatgtcgg
    27841 agaaaaaagg atttcataat gatgacgtgg tgttagatat ccacgatcgc ccaaccccag
    27901 gacattgggt aggattaagt ttacaacact tgttcacgat gttcggagca accgtgttag
    27961 tccccatctt agtaggtatc gatccaggta ttgcgctagt cagttcagga ctaggtacat
    28021 tggtgtactt atttactact aaaggaaaga tacctgcgta cttaggaagc agcttcgctt
    28081 tcattgcagc gatgcagctt ttgatgaaaa cagatggtta tccagcgatt gcacaaggag
    28141 cagtcacaac tggattagtc tatttgattg ttgcgctgat catcaaaaag atcggttcag
    28201 catggctgga taaaatcttg cctccaatcg tcgtcggtcc agtcatcatg gttatcgggt
    28261 tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa tcagggcacg tatgatttca
    28321 agtatattgc tgtagcgctg atcacattag gattaacaat tttcttcaac atgtggctaa
    28381 gaggttttct cggattgatt ccgattttac taggtattat tagcggatac atcgtagcct
    28441 tgctgtttgg aatcgtggat actaagccaa tcgtagacgc tgcctggttt gctgttccta
    28501 actttgagat tccctttgta caatatgaac caaagctata tcttggagca atcacaacaa
    28561 tggcacccat cgcttttgtg acgatgacag agcatatcgg ccatcttatg gtattgaata
    28621 aactaactaa acgtaatttc tttgaagaac cgggtcttca taagacgttg acaggtgatg
    28681 gattagcaca aatcgtggca gcctttgtcg gaggtcctcc tgtaacaagt tacggtgaaa
    28741 acattggtgt tctggctatc acacgtgtcc atagcgtgtt tgtgatcggt ggagctgcag
    28801 tatttgcagt cattttagga ttcgtaggaa aattaagcgc tttgatttta agtatccctg
    28861 gaccagtcat ctcaggaatc agttttatct tatttggagt aattgccgca agtggaatga
    28921 agattttaat cgagaacaaa atcgattttg ataaaaagaa aaatctgttg attgcttcag
    28981 ttatcttagt agtaggaatt ggcggactcg tctttgaagt aggcacattt acattgtcgg
    29041 caatggcatt agcaactgta cttggcatca ttttaaatct tatcttacca gaaacttcac
    29101 gaagcgaaga aaactaagga ggaaacatta tgatcatcaa atcagaacgt atcagcttaa
    29161 agcatttatt gaccgttgag gcacttactg atcaagaagt tatgggcttg atccgcagag
    29221 ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa gcgccagtac tttgctacaa
    29281 acctcttttt cgaaaatagt actcgcacac ataaaagctt cgatgtagcg gagaaaaaac
    29341 tagggctaga agtgattgaa tttgaagcaa gcacaagctc tgtccaaaaa ggggaaacac
    29401 tatacgatac agtactgacg atgtcagcat taggcgtaga cgtagcggtt atccgtcatg
    29461 gtgatgagaa ctattatgac gaactgatac aaagtaaaac aatccagtgt tcgattatca
    29521 atggaggaga cggaagcgga cagcatccaa cccaatgctt gcttgatttg atgaccattt
    29581 atgaagaatt tggtcatttt gaaggtctga aagttgccat cgtaggtgat atcactcact
    29641 ctcgcgtagc taaatccaat atgcagatgt taaaacgtct aggcgcacaa gtattcttct
    29701 caggtcctag agaatggtac gacgatgaat tcgaagtgta cggacattat atgccattgg
    29761 atgaactact agatcaagtt gatgtcatga tgatgcttcg ggtgcaacac gaacgccatg
    29821 atggaaaaga aagtttctca aaagaaggct accatcagga acatgggtta acagttgagc
    29881 gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc agcaccagtt aaccgtgatg
    29941 tagaattagc tgattcatta gtggaaggat tacagtcgcg gatcgttcag caaatgagca
    30001 atggagtatt cgtcagaatg gcaatcttag aagcagtgct aaatggaaaa gcgtaattat
    30061 aggaggatga aaaaatgaaa acactcatta aaaacggtca gatcaataca cgaaaaaata
    30121 tgacgacacc agcagaaatt tggatagaag atgggcgaat caaagcaatc ggtacaggat
    30181 tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg acaattgatc acacctggtt
    30241 tagtggacgt ccatgttcat ttgagagagc caggatttac atacaaggag acgatcgaag
    30301 ctgggactcg ctcagctgct cgtggcggtt tcactactgt atgcgcgatg ccgaatttaa
    30361 atcctgtacc tgatacggca gaaaagctga gacaagtata tgacatcatt agaaaagatg
    30421 cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa tctgcgaagt gagaaattag
    30481 ttgatcaaga agcactgatt gaagaaggtg cttttgcttt cacaaatgac ggtgtaggtg
    30541 tccaaacagc tgggacgatg tatctggcta tgaaagaagc tgcaaaaaac aataaagccc
    30601 tagtagccca tacagaagat gaatctttat tatttggcgg cgttatgcac gctggcaaga
    30661 aggcagaaga acttggtctt cccgggatct taagcgtaac cgagtcttct caaattgcta
    30721 gagatctgtt gctagctgaa gcaactggtg ttcattacca tgtttgccat gtttctacta
    30781 aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg aatccacgta acagcagaag
    30841 tttcacccca tcatcttatt ttgatcgacg aagatattcc cgaagatttc ggcttttgga
    30901 aaatgaaccc gccgctaaga ggacgggagg acagagaagc tttgatcgaa ggcttgcttg
    30961 acggtacgat cgattgcatc gctacagacc atgcccccca tggattagaa gaaaaaagcc
    31021 aaagtttcat gaaatcacca tttggtatcg taggaagcga aacagcgttt caactgatct
    31081 atactcattt tgtagaaact ggtcgattca cgttggaaca agtgatcaat tggttggcag
    31141 ttaaaccggc tgagattttt ggtttgaatg caggaacgct aacggttggt gcccccgctg
    31201 atgtagcagt ttttgatatt acacaaactt gcacgatcga taaagaagac tttttatcaa
    31261 aaggggagaa tacacctttt atcggctgga aagtaaaagg cgaaacacaa atgacttttg
    31321 tcaacggaaa acttgtgtgg cagaagggag aataaaagta tggaacgttt gcttatatta
    31381 gaagatggaa cagttttcga aggaaaggct tttggtgcag atgctagtgt cttcggagaa
    31441 atcgttttta caacaagtat gactggttat caagaaacta tcactgatca aagttttaac
    31501 ggtcaaatca ttacatttac ctatcctatg gtgggtaatt atggggtaaa tagagatgat
    31561 tatgaatcga tttctccaac atgcaaagga gtagtagtaa aagaacatgc acgtgtagct
    31621 tcaaactggc gaaaccaaat gacgctagat tcatttttga aacaaaaagg aattcctgga
    31681 atatcaggta tcgatacgcg cgcccttact cgcaaaatcc gtgaagtcgg aacgatgaaa
    31741 gctagtatag ttgatgctgg agattccttc gaacatgcat ttgatcagct gaaagcaacc
    31801 gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca aaccttatcc aagtccagga
    31861 acaggtcgga aagtcattgt cattgacttc ggcttgaaac atagtatttt acgagaattg
    31921 agtaaaagaa actgtaacct cacagtactt ccctataaca cagatgccga gacgatttta
    31981 gcgctatcac cagacggagt catgctgacg aatggacctg gtgatccaaa agatgtgcca
    32041 gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac cgatttttgg tatttgtctc
    32101 ggtcaccagt tatttgcttt agccaacggt gcagatacct ataaaatgaa attcggacat
    32161 cgaggattaa atcacccggt acgagaagta gcgaccggaa gaattgattt tacttctcaa
    32221 aatcatggct atgcagtcga tgaagccacg attgatccag aaaaactcat cgtaactcat
    32281 gtagaagtaa atgacggaac agtagaagga gtcaaacaca gagattatcc cgctttcagt
    32341 gtgcagtttc atccagatgc tgcccctggt ccgcatgatg cactacattt atttgatgaa
    32401 tttatggaaa tgatggacgc aggaaaggag cagtaaacag atgccgaaac gtacagatat
    32461 caagaaaatt atggtgattg gatctggtcc gatcatcatt ggtcaagccg cagaatttga
    32521 ctacgcagga acacaagcct gccttgcctt aaaagaagaa ggatatgaag tggttttagt
    32581 aaattctaat cctgcgacga tcatgacgga taaagaagtg gcagatcaag tatatatcga
    32641 accgatcaca cttgaatttg tatcaaggat tcttcgaaaa gagcgtccag atgctctcct
    32701 tccaacttta ggcggacaga caggcctcaa tatggcaatg gagctggcag catcaggtat
    32761 tttagaagaa ttgaacatag aacttctagg aacaaagctt tctgcgatcg atcaagcaga
    32821 agatcgtgat ctattcaaac agttgatgaa agaattgaat cagccaatcc ccgagtctga
    32881 aatcgtcaat acagtagaag aagctgtaga ttttgcaaat aaaatcggct atcctgtcat
    32941 cgttcgtcca gcctttactt taggcggaac tggtggaggt atgtgtgaaa atgaagaaga
    33001 attacgtatc gtatcagaaa atggattaag tctttccccc gctacccaat gtttgattga
    33061 acgaagtatt gcaggtttta aagaaattga atatgaagtg atgagagatt cggctgataa
    33121 tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg ggtatccata caggtgattc
    33181 tatcgtattt gcccctagtc aaacattgtc tgaccatgaa tatcaaatgc ttcgtgatgc
    33241 ttctctttcg attattcgag cacttaagat cgagggaggc tgtaatgtcc aactcgctct
    33301 tgatccacat agttttaatt attatgtgat tgaagtcaat ccgcgtgttt ctcgttcatc
    33361 tgcgttagca agtaaggcga ctggctatcc gatcgctaag ctagctgcta aaatagcgct
    33421 tggattaaca ttagacgaaa tgaaaaatcc tgttacagga acgacttttg cagaatttga
    33481 acctgcatta gattatgttg tagctaaaat tcctcgctgg ccgtttgata agtttgaaaa
    33541 cggagaacgg cttttaggca cgcagatgaa agcaactggt gaagtgatgg ctatcggtcg
    33601 gaatatcgaa gagtctttat tgaaagctgt gcgttcttta gaaatcggta cacaccatat
    33661 cgaacttcct gagttggcag acatcacgga cgatgaccta atccaaaaag tggttcgagc
    33721 acaagatgat cgcctgtttt atgtagctga agctattaga agagggtaca ccatagaaga
    33781 attagcggat ttgacaaaaa tcgatttatt ctttttagat aaactgttgc atatcgtaga
    33841 aatcgaacag cagttgaaac agcagccgtt ggatgacaag atattgaaaa cagcgaaaca
    33901 aaacggtttt acagacaaaa aagtggcgga gctgtgggaa ataaacgaag aagccgttcg
    33961 ccagatgaga aaagaaaatg atttgattcc tgtatataag atggttgaca cttgtgcagc
    34021 ggaattcgaa tcaaaaacac cttattttta tagttcctat gaatatgaaa atgaaagttc
    34081 acgatcagaa aagccctcaa ttctagtact gggatctggt cctatccgta tcggacaagg
    34141 ggttgaattt gactatgcga ctgtgcattc tgtcaaagca atccagcagg caggatacga
    34201 agcaatcatc atgaacagca atcctgaaac agtttcgact gacttttcta tttcggataa
    34261 attatatttt gagccattga ccttcgaaga tgtgatgaat gtgatcgagc tagaacagcc
    34321 gataggagta atcgttcaat ttggtggtca gacagcaatc aacttagccg aacctttgac
    34381 tgctgctggt gtaaaaatct tagggacaac gatcgaagac ttggatcgtg cggaaaatcg
    34441 tgatttattc gagcaagcgc tgaaagagtt ggatattcct caacctttag gggatactgc
    34501 tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt ggttatccag ttcttgttcg
    34561 tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt gaaaaccagc gagatttaga
    34621 agattacatg gaacatgctg tgaaagcatc tccagaacat ccagtactag tggaccgtta
    34681 cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac ggtgaaacag tactcatccc
    34741 agggatcatg gagcatattg agcgtgctgg tgtccattca ggggattcaa tggctgtgta
    34801 tccacctcaa acattatctg aatcgatcaa gaaaacgatc gaagattaca cgatccgctt
    34861 agcattaggt ttgaattgtg tcgggatgat gaatatccag tttgtcatcc atgaaaatga
    34921 ggtttatgtg atcgaagtca atcctcgagc aagtcggact gttccattct taagtaaggt
    34981 gacaggaatt ccaatggcac aaattgcgac gaaagctatt ttgggagaga aactaaagac
    35041 acttggttat gaaaacggac tttatccaga aacaaaagaa gttcatgtta aagcacctgt
    35101 cttttctttt acgaaattgc agaaagttga tacttactta gggcctgaaa tgaaatcgac
    35161 tggagaagta atgggatctg acagacaatt agacaaagct ttgtacaagg cattcgaagc
    35221 ttctggatta cacttgcctg aatatggagc cgtattattt acgattgcgg acgaaacaaa
    35281 agaagaagca ctagaattag ctaaacgttt tacagaaatt ggctatagtc tgataggaac
    35341 gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc gttgaaacag tagcgaaaat
    35401 ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata agagatggta aaacacaagt
    35461 ggttgtcaat acgattgata aagatcgggg aaatgcttct aaagacggtt tcatcatccg
    35521 aagagaagca gttgaacatg gtattccatt gtttacttct ttagacacag cagatgcgat
    35581 cgttcgagtg atggaatccc gtgctttttc aactaaccct atttaacggc gagcacatgt
    35641 taagacaaaa agacgaaaaa ccgttgcagg aggggacaga tgaaacaaga aatgatggaa
    35701 atcgttcagc aaaaacagct ggcaccaaag atttttgaaa tgacattgaa aggtgatcta
    35761 gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc tcgtgccgcg tgcagacttg
    35821 ctattacgcc gaccgattag cttgaatcat atcgaccacg aaacaaatac ttgccgtatc
    35881 atttatcggg tggaaggtga ggggactaga gtcttctcta ctctttctgc cggagaccat
    35941 ttagatgtca tgggtccttt aggcaatggt tttgatttgt ccaatcttca tacaggagac
    36001 gaagtattta taatcggtgg aggaatcggt gtccctcctc tatatgagtt atcaaaacag
    36061 ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt ttgccacaca tgaagtcgtt
    36121 tattatgaaa aagaatttaa tcgtctagga gaaacaagaa tcgctactga cgatggaaca
    36181 tacggtatgc acggaaatgt cggtaattta ttgttaggaa cgaagacgga acctacagct
    36241 gtttttgcat gtggttcaaa cgggttattg aagacagtgg aacagctgtt ttcttcgcat
    36301 caaaatgtcc agttatcatt agaatcccgc atggcatgtg ggataggggc ttgctatgct
    36361 tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa gtgtgaaagt ttgtgacgaa
    36421 ggaccgattt ttaaaatagg agaggtaatt atatgaatga gaatccttta gcggtcaaac
    36481 ttcctggcct agaattaaaa aatcctatca tgccggcaag cggctgtttc ggttttggca
    36541 aagaatacgg aaaatactat gatttgaatc agcttggatc gatcatggtc aaggcaacga
    36601 ctccaaatgc ccgctttggc aatcccacgc caagagtggc ggaaacacca tctggcatgt
    36661 tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt tatgcataaa cttcttccag
    36721 aattagagga gtacaatgag ttacccatca tcgcaaatgt tgctggtgct tgtgaagaag
    36781 actatgtaga agtttgtagc aaaattggcg aagcaccaaa tgtgaaagca atcgagctga
    36841 acatttcttg cccaaatgtc aaacatggcg gaattgcttt tggaacagat tctgatgttg
    36901 cttttcaatt aacacaagca gtaaagaaag tttcttctgt tcctgtctat gtcaaattgt
    36961 ccccaaatgt aacagacatc gttccaatcg ctcaggcaat cgaagctgga ggggcagatg
    37021 ggtttacgat gatcaatact cttcttggta tgcggattga ccttaagaca agaaaaccga
    37081 ttttagccaa tcaaacaggt ggactctccg gaccagcaat caagcctgtc gctattcgtt
    37141 tgattcacca agtagcaagt atatccagcc ttccaatcat tgggatgggc ggcgtgcaga
    37201 cagtggatga tgttcttgag atgtttatgg ctggagcaag tgctgtagct atagggaccg
    37261 cgaatttcac cgatccgtat atctgtccta aactaattaa agagctccct gtgcgaatga
    37321 gcgaactagg catcgaatcg ctggaaagat taatcaaaga agtgagagag gaacgtgaac
    37381 gatgaatcaa ctagaccaac gaccaattat tgcattagat ttttcaacta ggcaagaggt
    37441 agaagacttt cttcgatttt tcccgaaaga agaaaaattg tttgtgaaaa tcggaatgga
    37501 attgttctac caagaaggac ctgaaatcgt acgttatcta aaagaggctg gtcataatgt
    37561 atttttagat ctgaaacttc acgatattcc gaatacggtc gaaaaagcaa tgcggggatt
    37621 ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct ggcggtattc gtatgatgga
    37681 ggcggcaatg cgaggattag aagaggggac tccagaaggt ggaaaacgtc cgctacttct
    37741 agctatcact cagcttactt ctacaagtga agaagaaatg catgccgatc aactaatcga
    37801 agtttcttta gaaaaaagtg tgatccatta tgctagctgt gccaaaaaag caggattaga
    37861 tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa gaaatggcag gagacgaatt
    37921 tgtttgctta actccgggta taagaccaga aggaacagtc gcaggagatc agacaagagt
    37981 agtcacacct tctcaggcaa aaaagatcgg ctcaactttt atcgttatag gtcgaccaat
    38041 cactcagtca actaatcctt ataaagcata tcaaacgatt caaacagaat ggagctaacc
    38101 taaaatgaat gttgaacaaa gtatcgcaaa agacttatta gaaattgaag cagtattttt
    38161 aaatccaagt aatcctttta catgggcaag tggaatcaaa agcccaattt actgtgataa
    38221 ccgaatcacg atgagctatc caaaagtccg aaaagagatc gcaaaaggat tagcaagcaa
    38281 aatcaaagaa gcttaccctg aagtacaagt gattgctgga actgcaacag caggaatccc
    38341 tcatgccgca tgggtagcgg aaatactgga tttgccaatg gtctatattc gaagtaaggc
    38401 gaaagatcat gggaaaggta accaaatcga aggacgtata tttgaaggtc agaagatggt
    38461 tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg gaagcagctg aagcagcaaa
    38521 aagagaaggt gcggatatcc ttggtgttgc ggctatcttt acgtatgagt tgccaaaagg
    38581 aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg ttgaccaatt attctgtatt
    38641 gattgaagca gctttagaag atcgttatat cgatgagcaa gaattaactc tgctaaaaga
    38701 atggaaaaaa gatcctgaaa attggcagat tgaagcgcgc ttgatgaaga tagagaaatc
    38761 cgatttgtgt ctccagaatc atcaattgga atagcaaaaa tccaaaaact gattgcgaga
    38821 agcatctttt catcaggtat gaaaagaaac ttctatcgtg atcagttttt gattttttta
    38881 aaattcattt ttctaagact ggttagcaga aagaagagaa taatctcttc tcttttttct
    38941 attatatata gattttctct attttttggt aagggagctg tttcaaaaat ggtaaagagc
    39001 ggaaatgttg cgaatatcag ttatttaatg atatgatatt cggaaaaaga aatctcaaag
    39061 ggaggtaact tttatggggt tgaatgaatt tattttagga ttgaacaatt tagagacgat
    39121 cacaagggca cctggatttt ttaaatatac agagcataca gttgcagcac attcgtatcg
    39181 tgtagcatct attgcgcaag ttctaggaga cattgaagaa gcgaatggac aaccgatcga
    39241 ttggaaactg ctctatgaaa aatcattgaa tcatgactac acagagcgtt ttattggtga
    39301 tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga gggatgctgc aaacagttga
    39361 agaaaagatg acggacgaat tcatcaacca agagattcca gctgagttcc aagaagttta
    39421 tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa ggagaactat tagccatttc
    39481 agataaggtt gaccttcttt atgaatcttt tgatgaaatc atcaaaaata acccggaaaa
    39541 agtttataag gagatgttca ttgaggcagt gactacgatt aaaagctttg aacatcggcc
    39601 ttctgttcag tatttcttta aagagatatt tccggaacta ttgaaccagg aattttatgg
    39661 aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag aaagaagaaa aataaaaatg
    39721 atatgagatg cagggggaag tatagttgaa aaagaatatg gatgtcgaat ggggctattc
    39781 tggagacaaa ggtccggaaa attgggcgag tttatgtgat tggtttgcga gaggtgcgga
    39841 atttccattg cagtcgccta ttcatttgac caagggggaa gaaaccaaaa tagaaggaga
    39901 cacgctttcc tttcattatc aaaagcaacg gtttactgac aaggaattca aaaacacgat
    39961 tcatttagta ccttttgacc aactcagtta tgtagagttc aagaaagaac gctattattt
    40021 gacagatatt catttccact tgccttctga acatatcatc aacggaaaac aggaagatat
    40081 tgaatttcat tttgttcata tgaacagcaa aaaagagaat ctagttgtgg gtgtcatgta
    40141 ccagctaatg gtacaggaag gttggctgta taatcaagaa aatggggaaa gttggaactt
    40201 agaaaaacat gagcattggg taaatccgga tgtgttcttt ccggagcaaa aaagccattt
    40261 ccattatatt ggctcattga caactccgcc taccagcggg ccaatccaat ggtttgtaat
    40321 ggatcatgta ggaaaactga accaagagtt tcttctcccc ttcgatggtc agtatgctcg
    40381 accgaacaac cgaccaatcc agcccttgaa tggaagagaa atttattatt tcgaggagtt
    40441 tgagcaatga atattcagca gatgaaatac gtggtcgctg tagcaaacaa tggtagtttc
    40501 agagaagcgg caaagaaaat gtttgtctcc cagccaagtc tttcagctgg tatcaaagaa
    40561 ttagaaaccg agttagggat cacgctgttt actcgaacga atcgaggggc atatttaaca
    40621 gaagaagggc aagacttctt aaatcgtgcc gagaaaatat tggtccagct tgatacatta
    40681 gaaactcact atctaacgag tgaaaagaga gacaattttt cgatcgctgc ccagcattat
    40741 gatttcttag gtcctttgac cgcacagcta ctcgaggaat tcaaagacga gatcaaacaa
    40801 tttcggatca tggagacgac aacagcgaga gtgatcgaag aagtcaaaga gcagcacagt
    40861 gagatcggca ttatttatat gaatgagcat aatcaagctg gtatcagaag atatttacaa
    40921 caaggagagc taagtggccg cagcattgga aaattcaaaa cgcatatttt tttaaggcaa
    40981 ggacatccat tagctgaaca cgcagagatc accaaagaag aattgttatc ttatcctcaa
    41041 gttcgtttta cacaggatgg aaataatttc ccgtattttt atgaagacct tatcgagatc
    41101 cctgatcaag aaacagtgat ttatacgagt gatcgaggta ctttgatgaa tatcgttctt
    41161 gaaactgatg cttacgcttc aggatctggt attgtgattg gagggattcg cgagcacttg
    41221 cggttaatcc ctttggcaga aggcgaacta aatgaatttt atatcattta ttcggcgaaa
    41281 cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc ttactcggtt actggaccag
    41341 cagagctaag aaaagcagat gagaacgctg tttattgaac accgttgatc aaaaaagagg
    41401 ggttgtgaca gaattttgtc acaactcctc tttttgtagc aaacaattta taactaattg
    41461 ctgttgtggg aaagtacgca aatatgattc tagaaacact cattgattac ctgttttgtt
    41521 tcatagctgt aattttttct tcttctggtg tcacgataat cgtcttttgg ttttcataaa
    41581 taacataacc aggcttggcg ccatttggtt ttcggatatg ctttacctgt acaagatcaa
    41641 ccggcacttg ggcagagtat cggtattttg aaaaataggc agctaactcg gcagcttctg
    41701 taattgtttc atctgagggc ttgtcacttt taatgatgac gtgcgaaccg ggtatatttt
    41761 ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat tgtcagctgg tcattttgaa
    41821 gattgttttt accaacaaga atgagcgttc cgtcagaaga aaagtattga tcaggctgag
    41881 atggtttctt acgtttttgt ttttttaagt tcttcttttt caggtatcct tctgaagtca
    41941 attcttcttt aattgcttcg atatccattg ggccggcaat ttccagttga gataagacag
    42001 attcaagata ttggatttca tctttggctt cttggatttg ttcgccaatc agcttaacag
    42061 cgttcttcag cttttggtat cgatggaaat atttctgtgc attttgattt ggtgtcaatg
    42121 ccggatctag tgctattttg attggccgat cttcttcata ataattaggt aaaacaactt
    42181 cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag tagttcgcca tttctacggt
    42241 aattctcagc gttttcggat tctttgagtg tttgttcacg ttttttcaat ttatttttat
    42301 ttcgttttag ttcattttcg atttttcgaa tcagttctcc gccttgttgt tttgcccgat
    42361 ctttctctgc tttttcgtga taatagctat ctaataattg actgagtgtt ggataagctg
    42421 ttacagcaga tgcctgttcg gacaatacct gataagcaat cggtgtgaaa aaatctttat
    42481 tttccgtttc gtaaaaagta ggaataggct gatggctgat ggctgagaaa aattcgtccc
    42541 atacaaccat cttttcatta ggacgttcca gtaatctttt ggtcagctct tgagcgatat
    42601 cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg atctagttca gtttgggaaa
    42661 gccgctgaaa gattttttct ttctcggagc taaatgggtt caagacctct tgttttggcg
    42721 gagcaacata ttctacacca ggaagcaaag aacgataagt gttttgagag cttccaatgt
    42781 gtttgatcgc atccagtatt tttccagttt ctcgattaac taagacgatc gtactatgac
    42841 gtcccattaa ttcaacgatc aatatgatat tttgtagatc acccaactcg tttcgtttag
    42901 caaagtggaa atggatcact cgatcatttt cgatttgttc gatgtcttct aaaatagcgc
    42961 catccaaata ttttcgaagc atcatcacga agttaggcgg attatcagga ttctgataat
    43021 cgatttgggt aatctgtaca cgagcataac ttgggtgcgc agataataat aatcgatggt
    43081 ttttgccgcg tgaacgaatc accagcacga tttcattttc ataaggctga tggattttcg
    43141 agatccggcc agatagtaac gtttcacgca attcgttgac catcgcgtgg gtaaatacac
    43201 catcaaagga catggttcat tcctcatttc ttttcataag tatattcatt ataacgaaaa
    43261 aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag tctagaaaaa tcttggacta
    43321 attacataaa aaacagtgtt tctatcgttg aaatccaaat ttttaggt
//
LOCUS       DARBFD010000013        43049 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000013 DARBFD010000000
VERSION     DARBFD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.013"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            212..604
                     /locus_tag="SCK08_000708"
     CDS             212..604
                     /locus_tag="SCK08_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1200620.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="SCK08_000709"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="SCK08_000709"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEG1200621.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="SCK08_000710"
     CDS             3165..4775
                     /locus_tag="SCK08_000710"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEG1200622.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="SCK08_000711"
     CDS             5067..5936
                     /locus_tag="SCK08_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEG1200623.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="SCK08_000712"
     CDS             6101..7372
                     /locus_tag="SCK08_000712"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1200624.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="SCK08_000713"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="SCK08_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEG1200625.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="SCK08_000714"
     CDS             8763..9026
                     /locus_tag="SCK08_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEG1200626.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="SCK08_000715"
     CDS             9471..9920
                     /locus_tag="SCK08_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1200627.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="SCK08_000716"
     CDS             9977..11395
                     /locus_tag="SCK08_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1200628.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="SCK08_000717"
     CDS             11395..12255
                     /locus_tag="SCK08_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEG1200629.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="SCK08_000718"
     CDS             12429..13202
                     /locus_tag="SCK08_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1200630.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="SCK08_000719"
     CDS             13349..13714
                     /locus_tag="SCK08_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEG1200631.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="SCK08_000720"
     CDS             complement(13862..15070)
                     /locus_tag="SCK08_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1200632.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="SCK08_000721"
     CDS             complement(15089..15367)
                     /locus_tag="SCK08_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200633.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="SCK08_000722"
     CDS             15521..16216
                     /locus_tag="SCK08_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1200634.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="SCK08_000723"
     CDS             complement(16658..17455)
                     /locus_tag="SCK08_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEG1200635.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="SCK08_000724"
     CDS             complement(17452..18636)
                     /locus_tag="SCK08_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1200636.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="SCK08_000725"
     CDS             complement(18661..19908)
                     /locus_tag="SCK08_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1200637.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="SCK08_000726"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="SCK08_000726"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEG1200638.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="SCK08_000727"
     CDS             21888..22202
                     /locus_tag="SCK08_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEG1200639.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="SCK08_000728"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="SCK08_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEG1200640.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="SCK08_000729"
     CDS             22887..23531
                     /locus_tag="SCK08_000729"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEG1200641.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="SCK08_000730"
     CDS             23546..23875
                     /locus_tag="SCK08_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEG1200642.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="SCK08_000731"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="SCK08_000731"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEG1200643.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="SCK08_000732"
     CDS             24863..25687
                     /locus_tag="SCK08_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEG1200644.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="SCK08_000733"
     CDS             25680..26027
                     /locus_tag="SCK08_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEG1200645.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="SCK08_000734"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="SCK08_000734"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEG1200646.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="SCK08_000735"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="SCK08_000735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEG1200647.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCK08_000736"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCK08_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEG1200648.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCK08_000737"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCK08_000737"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEG1200649.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="SCK08_000738"
     CDS             31657..32451
                     /locus_tag="SCK08_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1200650.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="SCK08_000739"
     CDS             32441..34483
                     /locus_tag="SCK08_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200651.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="SCK08_000740"
     CDS             34764..34913
                     /locus_tag="SCK08_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEG1200652.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="SCK08_000741"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="SCK08_000741"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEG1200653.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="SCK08_000742"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="SCK08_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEG1200654.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="SCK08_000743"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="SCK08_000743"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEG1200655.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="SCK08_000744"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="SCK08_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEG1200656.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="SCK08_000745"
     CDS             39330..40412
                     /locus_tag="SCK08_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEG1200657.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="SCK08_000746"
     CDS             40412..41215
                     /locus_tag="SCK08_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1200658.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="SCK08_000747"
     CDS             41285..42025
                     /locus_tag="SCK08_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1200659.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="SCK08_000748"
     CDS             42036..43040
                     /locus_tag="SCK08_000748"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEG1200660.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT        13844 a         7520 c         9001 g        12684 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaa
//
LOCUS       DARBFD010000014        37857 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000014 DARBFD010000000
VERSION     DARBFD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.014"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(266..1210)
                     /locus_tag="SCK08_000749"
     CDS             complement(266..1210)
                     /locus_tag="SCK08_000749"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1200661.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1506..2450)
                     /locus_tag="SCK08_000750"
     CDS             complement(1506..2450)
                     /locus_tag="SCK08_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1200662.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2671..3885
                     /locus_tag="SCK08_000751"
     CDS             2671..3885
                     /locus_tag="SCK08_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1200663.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3907..5922
                     /locus_tag="SCK08_000752"
     CDS             3907..5922
                     /locus_tag="SCK08_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEG1200664.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6005..7378)
                     /locus_tag="SCK08_000753"
     CDS             complement(6005..7378)
                     /locus_tag="SCK08_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1200665.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7404..7688)
                     /locus_tag="SCK08_000754"
     CDS             complement(7404..7688)
                     /locus_tag="SCK08_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1200666.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7690..9705)
                     /locus_tag="SCK08_000755"
     CDS             complement(7690..9705)
                     /locus_tag="SCK08_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1200667.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9884..10186)
                     /locus_tag="SCK08_000756"
     CDS             complement(9884..10186)
                     /locus_tag="SCK08_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEG1200668.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10275..11306)
                     /locus_tag="SCK08_000757"
     CDS             complement(10275..11306)
                     /locus_tag="SCK08_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1200669.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11513..12388
                     /locus_tag="SCK08_000758"
     CDS             11513..12388
                     /locus_tag="SCK08_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1200670.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12540..13382)
                     /locus_tag="SCK08_000759"
     CDS             complement(12540..13382)
                     /locus_tag="SCK08_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1200671.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13474..15351)
                     /locus_tag="SCK08_000760"
     CDS             complement(13474..15351)
                     /locus_tag="SCK08_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1200672.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15348..16769)
                     /locus_tag="SCK08_000761"
     CDS             complement(15348..16769)
                     /locus_tag="SCK08_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1200673.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16772..20161)
                     /locus_tag="SCK08_000762"
     CDS             complement(16772..20161)
                     /locus_tag="SCK08_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEG1200674.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20418..21809
                     /locus_tag="SCK08_000763"
     CDS             20418..21809
                     /locus_tag="SCK08_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1200675.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21882..22529)
                     /locus_tag="SCK08_000764"
     CDS             complement(21882..22529)
                     /locus_tag="SCK08_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1200676.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22537..23223)
                     /locus_tag="SCK08_000765"
     CDS             complement(22537..23223)
                     /locus_tag="SCK08_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1200677.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23229..24302)
                     /locus_tag="SCK08_000766"
     CDS             complement(23229..24302)
                     /locus_tag="SCK08_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200678.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24524..25198
                     /locus_tag="SCK08_000767"
     CDS             24524..25198
                     /locus_tag="SCK08_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1200679.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25203..26543
                     /locus_tag="SCK08_000768"
     CDS             25203..26543
                     /locus_tag="SCK08_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1200680.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26692..27678)
                     /locus_tag="SCK08_000769"
     CDS             complement(26692..27678)
                     /locus_tag="SCK08_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEG1200681.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28402..29433
                     /locus_tag="SCK08_000770"
     CDS             28402..29433
                     /locus_tag="SCK08_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1200682.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29542..31074
                     /locus_tag="SCK08_000771"
     CDS             29542..31074
                     /locus_tag="SCK08_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEG1200683.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31213..32025
                     /locus_tag="SCK08_000772"
     CDS             31213..32025
                     /locus_tag="SCK08_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEG1200684.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            32015..32500
                     /locus_tag="SCK08_000773"
     CDS             32015..32500
                     /locus_tag="SCK08_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1200685.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32523..33068
                     /locus_tag="SCK08_000774"
     CDS             32523..33068
                     /locus_tag="SCK08_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEG1200686.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33141..34784)
                     /locus_tag="SCK08_000775"
     CDS             complement(33141..34784)
                     /locus_tag="SCK08_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1200687.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(34913..37141)
                     /locus_tag="SCK08_000776"
     CDS             complement(34913..37141)
                     /locus_tag="SCK08_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1200688.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37174..37653)
                     /locus_tag="SCK08_000777"
     CDS             complement(37174..37653)
                     /locus_tag="SCK08_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200689.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11036 a         7171 c         6898 g        12752 t
ORIGIN      
        1 aatatgaacg aattagatat gaatcattgt atttgccgtt gcggaaggat tttttaatta
       61 accacagata aacattgata acgattcgct tattgtaatc aacagattaa atttcatatg
      121 ttcatatgta cggatacatt ttcggaccga ttaattttaa ccattctgtt tatttgctta
      181 cctattagat atcaataaaa aactgcttaa cagctatttc agctgttaag cagttttagg
      241 atttttagtt catattcatt gtttcttata atacagtgtt caatacttct cttaaagcac
      301 ttgttgattt gctgaacatt gctttttctt cttcgttgat tgataattca acaacttcac
      361 gaacaccatt ttcgtcaacg attgatggca cacctgtaaa gatatctttt tcgtcgtatt
      421 caccagttaa ataagcagat acaggcaata cagcttgttc gttattcaag atagctttta
      481 cgatacgtgc agtactcata ccaataccat agtaagttgc ttttttacga tcaatgatct
      541 cataagcagc attacggact ttatcagcaa tcacatctaa ttcatcttgg gcaatacgat
      601 ggtctttttc tacaatttca aacactggtt taccaccgac agttgtatgt gaccatgcag
      661 caacttctga atcaccatgt tcacctaaga tatagccgtg gacgctgcgt gggtcaactt
      721 ttaatttcaa tgcgatttct ttacggaaac gagttgtatc caatgttgtt ccagtaccga
      781 taacacgtga tgttggcaga ccagattcgt tccaagcgat atatgtcaaa atatctactg
      841 ggtttgaagc aacaacgata ataccgtcga atcctgatcc catgatttct tttacgattt
      901 ggcgcatgat tgacgcattt gttttaacta aatctaaacg agattgacca ggtttttggt
      961 tgataccagc tgtgataaca acgatatcag catctttaca ttcttcatag ccacctgacc
     1021 atacagccac attttcatcg ccccaagcca taccatctaa aaggtccaat gcttcccctt
     1081 ctgctttttc ttgattcaca tcgatcaata ctaattcatt agagattcct tggttgatca
     1141 ttgcataagc gatacttgtt ccaacaaaac ctgtaccgac gattactact ttacgacttg
     1201 tttttttcat gaataaacag ctccttttca taactatttg ttactttcat gaaaagtata
     1261 gtcttgttta ttcattttat caacatgtta attataatag ttgttattat aatttttgat
     1321 ggaaacgaat tcctgaatac gcataacaga taaatgacaa atgtttacac ctatatcccc
     1381 tgataataca accaaaagtt tagaactgaa aataaatcca aaaaaattcg ctgcaacgat
     1441 ataataagac agcaatcgca cctaaaacaa ttttccttca tatgctttag tgaaagattt
     1501 tcttcttaat gtttatttct ccattcattg ggagtacatg ttgaatactt cttgaataat
     1561 ttgaataaag aagtcgtttg tttatatccg atacgattag cgatttcttc caacgaaaaa
     1621 ctcgtttctt gaatcagttc caacgcaaag tcgaattttt tctttagaac cagttcttga
     1681 aaggaagcat caaaatacat tttcaattta ttactaagat agaccggatt gtagccaaaa
     1741 tgagctgctg tttcttttaa cgtacaagaa gtaaaatgct tatcgatata atctaatact
     1801 ctaagcatct cattttctgc gcatccatct gctgatgaaa agctttgttg ttctaataaa
     1861 tgagtcaatt cgatcagtaa tagtgatgta tagagattta acgaacggat cttatgactg
     1921 tatgtatcaa aataaatcaa cagcatattt tcaatcaaat gcgtcaaacg acttgttttt
     1981 ccgccgcgaa aatgaatgaa ttgtgtatgt tccccttttt cttttgatgc atttatcaaa
     2041 aaagaagaaa gtagattatt tgtgcttgcc aaatgattca atatctctgt agataaggtt
     2101 tcttctttta ataagatgtt gattaaaata tcctcttgcc ctgtgtattc gattctttga
     2161 gtagcttctt tatcaatgat caccacgtca ctttcttcca tctctatcct ttctccatta
     2221 ataatttgta aacatgtacc gctatacata taattgattt caacaaaatc atgtgtatgc
     2281 tcgggaactt ccgcatatcg acggtgttta ctgatatgaa tatttccctg ttgaaataga
     2341 tcccctcggt ggatttttgg cggtgtctga agatggtaag ctaagtttaa atcattgata
     2401 ttcattcctt gttcacgctg cagagtttcg attgattctt cgttacgcaa aaaaaatgtc
     2461 taattcagct agttccaatt gactccctcc tttttgtaag cggtttcttt cgcttattag
     2521 ttcacgctgt actgctatta tatgcaaagc tatttttggt ttcataaaga ctttctatgg
     2581 ttgggacatg aacttcctgt gattccgcta ttgagagaat aaccacttca tactatattt
     2641 ttgataagcg cttacaaaaa tgaaaggaac atgcttatga ataaaaagat gatgaaattt
     2701 tctctgctgt cagtctctct tattttgact tctgctggtg cagtcacgtc tgcaattccc
     2761 agtatgtcag ctacttatgc cacgcgttct ttatctgctg ttgaattatt aacgactgct
     2821 ccctctttta tggtcactat ttttgttttg attagtagtt ttattgctag gaaaatcgga
     2881 caaaaacaaa ctgtgatcat cggattagtg attgcatcga tttgtggtat cctccccctc
     2941 ttctcacaaa gttacagtat tgtcttagct tcaagagctg gactaggtat tggctttgga
     3001 ttgatcaatt ctttggcagt cagcatgatt gctgcctttt atcacggaga tgaacgagcc
     3061 tctatgattg gttttcaaag tgcatttcaa ggatttggtg cagctttaat gacatttgtt
     3121 gcaggacagc tagtacagtt cggttggcaa tatacctttt tagtctacgc catcagctta
     3181 cctattcttg tcttgtttgt tttgtttgtt cctacacctg aaacaactga acttcaatcg
     3241 catggcacag ccccaacaag acaagtaatc aaaacgaatc ctaaaatgat tagttatggt
     3301 atttttctct tgctggtcat cgtggtatat aatgctgtat cgattaaatt agccacagtc
     3361 ctgacgactt attctctagg aaacgaaact aatgcagcga atatcttaac aatcatgcaa
     3421 ttggcaagta tggctgccgg agtttgtttt ggctggcttc agacaaaagc acatcgctat
     3481 atgcttacac tttctttact actgatggga atcggctttg tattgattgc cactgtgata
     3541 aacctgtatg tgattgggtt tggtgcgatc ctaacaggaa tttctttttc gttattcatt
     3601 ccttacttat tcaaccaagt aagtatccaa gcgcccaagt acgcggagag tttcaatact
     3661 tcaatcttat tggttggcgc taatttaggt actttcattg caccatatgg tttgctcttt
     3721 ctctcttttg cttctcttgg gagtaaaaca cttgctgttt tcgtcaatgg cagtatcttg
     3781 ctgttgatta gcgcgcttat tagccttcta gtagtacaca ggacgaatag aaagacagtg
     3841 ataagagtag atgaagaaat aaaagaacaa ttgacaacaa aataaatttg atggaggtcg
     3901 tgtaaaatga caaatgaaaa acataaagat agatggcttt ggtatcctgg agactttgag
     3961 atccgccatg gactgctaca aaatttccaa cgggaagaac gaggttttga ctggccagcg
     4021 tactggtaca tggatgattg ccatcggaac gtcaagttta agagatatta ctttcttgat
     4081 caaccttcca tgtttaaagt gacgatccaa ggagttgggt atgtagaaat taatggacaa
     4141 aaacatccat gtggaaaatg gctgacttgt ccagctggta aagcaaaaat tcgtatcttt
     4201 gtaggacata catcagggct accagcgatg tttatcgagg gagacgaagt aaaatcagat
     4261 attggctgga cagcaagtaa ttttattgaa gaatatccag ctggctggag tccgctttat
     4321 gtcgatatcg caaaagatcc aaatcaaatc tattatcaaa aagaatgcgt ccatccagtc
     4381 actgaaacgg agattggcgg aggaatctta ttcgattttg ggcgagcagt gaatggctgt
     4441 gtagaactaa tatctctgga tgaccatatc gaatctatca ctctatgtta tggagagtcc
     4501 gatgtagaag ctatggatat cgattattgc tactataagc aaataggaat gacaagaaaa
     4561 atgaaagctc gtaaacgcgc ttttcgctat ttgtttatac cagaggtttc tactggaatg
     4621 gttcaacttc aagtttttca tgaattttta tctctttcga aacaagctgc tttttcaagc
     4681 gattcttctt tactgaatca gatctggtca gtatcagaag aagccttcaa gttatgtagt
     4741 gatttgtttt ttatcgacgg tatcaaacga gatcgctgga tttggtctgg agatgcgtat
     4801 caatcattca tgatcaatca atatcttttt tttgacgagg aaatcaataa acgaacgata
     4861 ttagcattaa gaggacaaaa tgaaatcaag cagcatatga atactatcgt tgactattcg
     4921 atcttgtggt tgattggtat cgaaaaccac tatatgatga cgaaagacta tgaattttta
     4981 cgcatcgtct accctaagat gcaatcatta atgagatact tgatggagca aacgaatgaa
     5041 ttagggttta tttatgggag agcaaaagat tggatattta ttgattggtc tgaaatggat
     5101 aaagaaggaa cgcttgctgc agaacaagtc ttattactga aagcctatca ttccatgatc
     5161 acttgcggag atatcttagc tgaaacatat ggagagaacg aaaattatca tactcatctc
     5221 tatcaagaaa aatatcaact attgaaacaa aacctatttg actacttttg ggatgaagaa
     5281 cttggcgctt ttatcgattg ctatgaatct gggagaagaa atgtgacgcg tcatgcgaat
     5341 attttcgctg ttttattcga ttttgctaac aaagaccaac agcaatcgat cttaaagaat
     5401 gtcttattga atgacactat cacacagatt actactccat atttcaaatt ctatgaacaa
     5461 gatgctttat gcaaactcgg acagacgaat atcgtctacc aaacgattct tgattattgg
     5521 ggagggatgt tatctcatga tgcggtaact ttctgggaag aatacgaccc tactcaaaca
     5581 ggaaatgaaa agtacagtat gtatggagat ccttatggaa aaagcttatg ccatgcatgg
     5641 ggagcaagcc caatctatct tttaggacgc tattttatcg gtttgtatcc aacaaagcca
     5701 ggttatgaag aatttatgat acaacctaat ctagattcat ttgaaacact gaattgtacg
     5761 ctacctatca aagatggaaa gatttcgctt gaaaaaacaa aagataagat cgtgattact
     5821 tctgatagaa caggcggaac agtgaaaatg aatggtaaaa gttatcctct tctagcaaaa
     5881 cagccacttg tcttaacaag ctattcaaaa ataaatgcat aaaacaaaaa actgcttgcg
     5941 acatatcaga agactttaaa tcttcttctg atattgtcca agcaattttt attttgaagt
     6001 ttgcttaatt ctgttcttta gaaacaatgt ttgttctttt tccggcaaaa agaaagctag
     6061 ataatacgac aagtaaaaag aaagcgatga ttccaactgt caaggctatt tgaccgtatt
     6121 ctcctagttt cccaagtaca agtcctggta ctagataatc agtatcggca aatgtagaag
     6181 aagcgatttt aagatctcct aatactggaa ggataaataa tggcagccaa ctaatcaata
     6241 agctattgaa agcagaacct aataccgccc ctcttctacc gccagtcgag ttgccgaaga
     6301 caccagaagc agctccgcag aagaaatgtc ctactacccc aggaatgata actgtagttt
     6361 gcaagcctat catagcaaac atggatagtg ttcctacaaa gaagctaaca aaaaagccaa
     6421 ttaatacagc attaggtgca tatggaaaaa cgataggaac atctaatgct ggtttagaat
     6481 cgggtacgag ttttttagca atcccttgga atgctggaac gatttctgcc aacaccattc
     6541 gaaccccttg aaggacgata acaaacccag ctgtgaatgt tccagcttgg atcagtgcaa
     6601 agattacagc gttgtctcca ttgcttagtt ctctttcaac aaatccagga ccagctaaca
     6661 aagcaagtgc gacatatacg atcgacatca agagcatgat cgatacagta gaatctcgca
     6721 aaaagctgaa acttttagga atctttatgt cctctgtaga acggtctttg tttccaaaaa
     6781 actttccgat ccatccacta gaagcataac cgatatttcc ggtatgccct aacgcaactt
     6841 gattgtttcc agtgatgttc ttcataaacg gttgacaaag agcaggcgta atagttaata
     6901 gtgttccttc gaagaccccg cctaataaga tcatccacaa atttgcagta aaccccgcgc
     6961 ttactaaaat gactgctgtt agacaagaaa cataaagcat tgcttgacct gtaagaaaaa
     7021 tgaatttata tcttgtgaat ctagcaagta aaatattcac gatcatccct actagcatga
     7081 tcaaagcagt aggtgtaccg tattgttgca aagcaatcgc cactactgct tcattgctag
     7141 gaactactcc ttgagtatgc agagctgttt ggaacatcga agcaaaaggg gttagtgaat
     7201 tactgattaa accagcaccg ccgatcagta ctaggaatcc gacaaatgtt ttgatagttc
     7261 cttggatcac ttctgaaaat ttcttcttct gaagaacgag accaagcagt gcaatcaaag
     7321 aaacgaggat ggagggctga ctgacgattg acacaaacaa attcaacaat ttatccattg
     7381 atagttcctc ccttgaatca tttttattct tttgacaaga tcttcgttac tttttctttt
     7441 acttcttcgc tgtcaatgat cgattgcaaa ggtacgagct tttcatcagg tatagaagtt
     7501 agcgtatctt tcaaagaatg ctcaatgaag aaataatcag ccatatcgct agatacactt
     7561 ccaacatccg tatgacttac ttcaaactcg tccacatctg ctccaagatc tgcaagtgct
     7621 tgtttgatgt tcatctctac cataaaactt gttcctaatc cactttgaca tactgcaaaa
     7681 attttcatat cattcgtctc cttttcttat gatcgctaat atttcttctt ttgttgatgc
     7741 gtgtaataaa tcttgaagat tttgtttatt agaaagtagc tttgttaaat ttgccaaagc
     7801 ccgaagatgt gcctcattat cgatagcagc taaacagatg aacaaactaa tgggatgggc
     7861 tggattatca agcagatcca caggttgttg aagtttcagc aaagacatcc ctacttcgtt
     7921 tacgccatct tctggacgag catgaggtaa tgcgatgtga tcgccaatat ggataaacgc
     7981 accatactgc tccacgcgct cgatcatagc tttgatataa ctttcttcta ttttattttc
     8041 tgataatagc ggctgtgcgg caaatgtgat tgcttcttgc cagttttttg gttgatccga
     8101 taattggatc atttcttctg ttaataattc agatagcata ggacgttgat cctccttctt
     8161 ttctaaaagt ttgttcattt tacgatttaa tacttgatag agcttttctt cgtctacgcc
     8221 ttcttttaat tcgatataag gcagtagcgc cgacaacaat tcttgggcag aaggcattga
     8281 aaatcctggt atcagccaat ctctttgtac ttgattgatc agctgatttt tttccaagtc
     8341 agtcatgatt ggatgcagaa catagagaga ctttttctta tcagtaagtg tgagaggtac
     8401 agtagaaaaa ataatatcgt aagaagtttc gtcaacctcg tgtaagcggt cagctgaatt
     8461 agtctctgta aaaaagatca tcgggaataa ttgctgaagt tcacttttta agatcaatga
     8521 agagctaatg ccacttggac agacgatcac ggctcttatt tttctatttc tttcttctgc
     8581 gtccgctttt ctgatctctc cgccaaatag tatagtgaaa aaaccaatct cttcttcagg
     8641 gatgctcttc cctattcgtc tttctaacgg aagtaatgct tttcttgtca tttcgtagat
     8701 ggaagcatat tgatgcttaa tttgatcgat catcacatta ggtaaataaa aagaatagga
     8761 aatacgaaaa taggcaggga ccaagtgata aaagacattc atcaataatt cacgaaacga
     8821 agcaaattct acggccatca ggaattccat gcgatggatg acttctgaag aacattccag
     8881 caaaaaatca agtgcaggct cctggacatc tccttcaacg atcgatgcta agcaaagaga
     8941 gaaaaatata atctccgaat tttgatgttc ggaatagcca aggccagtca atacttgtcg
     9001 actaatgtca gtgactgcaa gagaagccaa cagttcagca tcttttacca gttccatgga
     9061 tgtttttcca attcgaatgg ttgtcaaact caaataataa gctaattcat ccattcgatt
     9121 tggtgcgaat cgaagatctg atagtgaaat cgttttatct agcaaatctc taactttggc
     9181 gtaataatca atggaaaggg ttgcggttaa atacgtgagc ccaaatcttc cagcaagtga
     9241 ctgcatcaaa cgagcagtgc gattttttgc ttctcttctt aatattgctt ctgaccctag
     9301 cagcctaaaa ccagttttcc tagtatacga cagctgacaa tcttccactt ctaattcatt
     9361 tcttatcttt ttgatatctg acaggactgt ccctttagaa acctgaagaa aatcttgaaa
     9421 atgaaaaaca gaaacagacg ttgcagtcga ataaaccatc aaataaagaa gatctcttcg
     9481 ctcttgttcg gtatacacag cttcaaatcg agtaatatcc aaaaattttt ctgcccagtc
     9541 gattgctcgg aatttcttta tacggatacg ggtatcatcc acatattcgg caaaaggagt
     9601 taattgtttg atcgcctcgc ttgtatttac ttcgatgatc tctgctgctt tttctagact
     9661 gatttctctt ggtgttttca ggagataact aattttgtag ttcacagcgc tgttcacctc
     9721 ctacttatat tctagtatgg tagcgctttt aaatcgaggg gtgtttggta caacttacag
     9781 ttggattttt tttacgactg taccgaacga tttctatcga atggatacag aaaaaaagca
     9841 ggtgaccaaa tgtctcctgc ttttatgaat tgatttcgtc cgatcaatga tgatcatgtt
     9901 tattttttga ggtcagttct tcaaattttt ctttgacatc ttcaaccgct ttttcgatat
     9961 ctttttcaaa gtgattgggc tcattaggct ttgtttcctc ttctacagaa tttgaaccag
    10021 ataaagctgt ttctttattt tctgtttcac cagagtttgt gccagctaat gattcttctt
    10081 tgtcttctgt ttcaccagaa ttagttcctg ctaaagccac ttcttctgtt ttaggggcca
    10141 ttgaacctct taatcgtttg tgtttttcac cgtcataatt tgtcatgttg gttcctccta
    10201 gtagttctat actttttcat cgtacaataa gacagtttat gcgtcaaaaa acaaacccga
    10261 ttcttacaaa tatgtcatat ttttccttct gattcttgaa tgcttttgat tccttcttcc
    10321 aaaattttca tttgtaagat tgtttcttca tctttgatga ctttttctgc gccattaact
    10381 aatgtctcgt aaatcccttc gtagacacgg ctgtaatcac caacctcgga aattactttt
    10441 tcttcatgat aactcccttg ttcatctata taggtcaata caccataatg ttcgggcaaa
    10501 tcgataccaa agtcttcatg atcaggcatg taaaacattt tcagatgttc ttcttgtcga
    10561 tcttttgttt gtttcacgaa catccctttt tttccgtaaa cgacgaaact tggacgttct
    10621 ttgatgcgga aataactgga ttttatcgaa attttcatat ttccatagaa aaaatccaga
    10681 tcgaagtaat cattcattcg tcctttccct aaaagttgac gtacatcata gctgatatca
    10741 tctggttcgc cgaaataaga aagaacttga tctactgtat gacaagcatg tccatataaa
    10801 tagctgttcg cagtagaaaa ttcatgcgta ttttctggta cttccggtct gaaataatca
    10861 taatgcattt ctacttctaa aatatcccct aattttccgc tttcgattac tttttggact
    10921 gtcaagaagt cagaatcgaa ccgtctgttt tggtaacatt ggataaatag acctttttct
    10981 tttgctaatg caaataactc ttctgcttcc tttgacgttt ctgcaaaagg tttctctact
    11041 agtacattct taccatgttc gagtgccatt tttccatatt cataatgaaa ttgactaggt
    11101 gtagtgatga caactaattg gatctcagga tcatcccaaa gatcagacag ctgatcagta
    11161 tattcgatgt cttcgaatgt tttccacact ggttcagatg gggaaaaaat ttttttgata
    11221 ttgattttgt tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt actctttcca
    11281 ttgccaagat aagcgattgt aagcatgagt ggtgcctcct tttattgatg ttttcagtat
    11341 atcctagaaa agcgcagaaa gtatttataa tagctattct ggtaaaaaag ttcagaaaaa
    11401 cacttgttat cattcggttt ggtgaaatta atgttcaaag taaggataaa aatcaaaaag
    11461 atagtagcgg aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga tcatgcttat
    11521 tgaagaaaaa ctaacaaaac aagagttgtt taccacgaca gagaaacgga ttgtcgatta
    11581 catccgtagg aatatcgaag ctgcagtcta catgacgatt gaagaattag caaaagctac
    11641 gtatacttcc cattcagcta tcattcgttt atgcaaaaaa atgggattca gcggatttaa
    11701 agaattccgt ttcgagctgg ggcgggaagt tcatcaattg attgctcagt tcaatcaaac
    11761 gattgatcct aattttcctt tttcaagtga agatagcact cagcaaattg caaagaaaat
    11821 ggcggattta tcgatccagt caataaaaaa agcacaattg cagattgaac atgaagattt
    11881 agatcaaatc ggtaaagtat tgaacaaggc acagcgaatc tttctttttg caaaaggtga
    11941 ttctcaaatc acggcaagga aattccagaa taaaatggtc aagttgaata agtttttgat
    12001 catggctgaa gaatacagtg attcttcttg gaatgcagcc aatttgacta gcgaggattg
    12061 cgccattttt attagctaca gcggtcgcat ccatcattat gaacgaataa tgacctattt
    12121 gaagcatgta gaggccccta ccttgctgat taccggaaat cagcattcag aaatggccaa
    12181 acaggctgat atgtgtcttg ttatatcgca ggaggaatat gattttgcta aagtagcgac
    12241 tttctcctct caaattgcct tcgattacgt attaaatacg ttattctcag tgatatattc
    12301 tcaaaatttt gaagagaata tccgcaactt gaaaaacaaa caagaattga tgcaaaaagg
    12361 ccttcttaaa gaatatcgga aaacttaaga aaagatcctc atttagtaat aagaaagata
    12421 gtgtttgata ggctctcttt gatatcatca taaaaacagg ctgaagtttt ctttttcaaa
    12481 cctcagcctg tttgtttacg gaacgattcg aatagatata ttatagaaat gaggaatagc
    12541 taacttctgt ttcttatttt gaacgattga cgaaaaagat atataagaag gaggaaaatc
    12601 agtatacatg cagctaacaa tccatacagg aaaaatcgtt tgttctcgga tactttctgt
    12661 acttcttttt gaacttcttt tgcatgataa ggaacccggt gccctctgac aagtaagcga
    12721 tgactgttga ccatataagg tgtacaagtt aggagtgtca cgaaatcctg ccctttttcg
    12781 attctcagat cttttgtatc agtcggttct accgtcttga tctgatcgac ttgataagcg
    12841 agtgtccgtc cattgatttc gatatagaaa gaatcaccta aagtcagttt cggtaagtct
    12901 gtgaacaact ttgcttgagg cagtcctcga tggctggaaa taacggcatg tgtattttct
    12961 ccccccgtag gataagacgt tccttccaat aatgaggcac ctttttctag cagcacagta
    13021 ttcgtttcat caaaaattgg caaatggaca ttgatctcag ggatcgtcaa aataccgatc
    13081 gtatgtttct caaaataggt tctatccttt ttcgcaggtt cttttttctt agtaaaggga
    13141 tctgcacctg gatttccacc ttcttttgct aattgctggt ttttctttgt catgttttct
    13201 tggattttag ccatgacttc ttcattttct ttgaccgctt gttgctgata atgagagatg
    13261 atctgctgat ccagataatt attcagcgcg tcgctaacaa atggataagc tagtatccct
    13321 attcctgaga gaagcaagaa aagcatcagc agatctagaa gtttcctttt ctttggtttc
    13381 atttgtgttc tccttgtgat gactgtttga taataaaaga aaaaggagca tttgcgtaat
    13441 ccgctgctcc tttttccaat tcttgcaaaa agactaaatc tgactgtgct tgcgtctagc
    13501 aaagtacagt ccagcaatca gtagaaggac tactcctgca ccgatataca catagattcc
    13561 cttaccgcct gttgaaggaa gtgtaccttt gtgtttattt ggtatttttt caggagaaat
    13621 caactgtcct gctgttacat aagattgttc atcgatagta aatgctacac ggtttgttaa
    13681 tggaacatat ttttctggcg ctttcgtttc ttccagatag tacgtgccat attttagacc
    13741 tgtcacatcg attaaaccgt tacttgtggt tgtaaaaacc gttgctgatt ctttcgccga
    13801 tacccagctg acggcttttg tggatgggtc gatcgataaa tatttcgctg tgtcactatc
    13861 ttgatcacga acgacgaatt ctgctccagc aagtgtttgg tctgatgtaa cgtcaccatc
    13921 tactttaaca aatcgtttgc ccccagtaac gacatcgact gacggtggtg tttgatcatt
    13981 tgtatgcccg ttatcgacat ttgcttggtt agaaaatcct ttggttggat ctgctttttc
    14041 gttcaaatgc atatagtaaa cgaatttcaa tgtaccgcca ggagttaatg aaggaatata
    14101 attcggatca actgaaaccg tgaatccgtc tgtttgctca gtgacagaat aatttactgg
    14161 gtcgatttct ttatttccat catataaggc ataagcatac gttcctgaag aatcattatc
    14221 aaatgttaaa gcagcgtcat gagtatcgat aagtttgaat gttgtgtact tgttttgcgt
    14281 tccttcttta tcagcgatac ctaatgggat attgacagaa atctcatatt gaattttttc
    14341 accgattgcg acatcttttc cattcaattg aggtgttgtt ttatcaactt tagacgtatc
    14401 atttttgatg gtcttttcta ctggtgtttg gctatttgcc aggatctcaa aaggcgtgat
    14461 tgtttgcttt tcaatcattt ccgcattatc aggagctttt acttcttcta aattatattt
    14521 tccaacttct aaatgattaa cgatcaactg gcctttttca atagatgcct cggcaaagtc
    14581 attgttgccg atgtcataag aatgaccagt aatgaaatgt ttggctttgg tttgatcagt
    14641 tgtccaagtg tacaatccat ctgtgacact ttggatgtat tttttgacgc ttggtgttcc
    14701 ttcttcttta gaaataataa attctgctcc atttagtgct tcgttttcgg cagtaccgat
    14761 ttttgtaact ttcaacgttc catcattacc gactgtattt ttagggtaga gatggatagt
    14821 atctagttct tctgtcccgt atttataaga gccatctgct tgtttgatca tctcatatac
    14881 agggaaagct aaaaccatgt ttgcggcagc tgacactccg tcttttggtt cttctttgat
    14941 cgtgtagact gcatctttcc cattttgttt tttaggtaaa gataaagtga catttccatc
    15001 agcatctgtc gttcctgaag caactggtgt accaggagtc aaagactgga ctgcttgttt
    15061 tgctgcatcc acggacgctc ctttatctcg ttgcgcataa aattcttgag tgacgttata
    15121 aactgaaaat gaaatatcgg ctaatccttg gtattgatcg aattcgctca tttctttccc
    15181 gctgttttgg attaaaggat cgggtaaatc agtcattttc tttttatgaa ggatgacttg
    15241 agctgctgtc tcctctgcaa acactttttt tgcgccgaag ctgtttgtga gtaatggtaa
    15301 aataaggaaa aggactccta acataacgtt tattttttta tgatttttca ttttttcact
    15361 cctgttcttt ttttgataaa caaccagatt gcaaagcttg cgaataccat accaacaacg
    15421 cctaaaagtg tatttttcgt ttcgtttgtt tttggaagaa atggacggct attttctttt
    15481 ttattgacta ctttcaatat actgtccgcc tctttatcct cagaaagaaa agttgaaatg
    15541 gtaaacggaa tttctgtttc acttaaaata taaccagaag gtgcttcgat ctcttttaat
    15601 cgataacttc ctgttttcaa cccggaaatt gaaaaagcgc catttttatc ggaaatcagg
    15661 tgaagcgcat ctgattcgtt ctttgtccac ttgtatccgt tggctgtttg ctggaggtaa
    15721 tttcctgctt tgtttttaat aaggaatact gcgccagcta atcgtttatc tggtttattt
    15781 gtctcgattt tgacaaattg cttactccct gtatatacag agactttctt tgtactgaca
    15841 ccaaaaccaa aatccagtgt tccgttgttg ttgatttcct tgttcgcctg tgctgtactg
    15901 cttagctgca tctgataaga aatggtcagt ttttttccag catatttttc caacgaaggg
    15961 atcgaaaata aaacatcaaa tccgtgaggt ttcttgtgta tgacaaagcc ttctgtcagc
    16021 ttttctccat tcgatgaaat cgttaaacta ttaggtaaaa acgttaatgc ttcatccgca
    16081 ctatctgaca attcgaactt ttgatagtca aggatattta caggaattgt cgtatgcaag
    16141 gaataagtga ttttttcacc aatcgtaaaa tcatttctag gctcttctaa taccttctca
    16201 aaaggtggat cagggtagtc gttttcttca tttttaggat acaaatggat agttgaaagt
    16261 ttttgattgt tttgtccatg tacaggaaga acaactacca tattctctgc cttttctttg
    16321 acgacttctg gtactttgga ttcaatgaaa agatacgctt tatctctttt ttcttgatct
    16381 ttagcggcca gagaaaaaga agcgatacca tcttcgttgt taagagttgt agttgttgct
    16441 tctgcggtaa acataccgga agacgcaccg ttttttgcga tctctgcttg tgcttcttct
    16501 accgttttgc ccttttcccg tagatggtaa aaagaatctg tgacatcata aacttggaat
    16561 gtgacaccat ttaatcctcg atacgtttgt aataaagctt tttcttgtcc gtcatttgga
    16621 tgatttttcg gcagttgccc gtttgggaaa agcaatttgt gtaaagtaat ctgaaccatt
    16681 tctgtttctg tttctgttgc tacctgagta aaagctggtt taaatagtaa caagaagaga
    16741 cacatactaa gccaaccaag ttttttcata tttagctgcc ccctctttct tttttcaaaa
    16801 aaagatacca aatagaaaac gcacaaccaa tcatccctac tagatagatt cctaaacggc
    16861 caattcctcc cgtttcaggt aatggtactt ttgcctgatt cgtgatgtct aaagaaatct
    16921 ggttgttttt ggctcctggt gacagaacag attcatgatc ttgtccatcc acttgaattg
    16981 acccatcttc gtgtataact atagtaactg gctcttttag accttggtat ccttctggtg
    17041 taaaagtttc ggtcagcgta taagttccag gttttaaatt ttcgaataga aaggtatctg
    17101 tttcttgtcc atcttttggc gattctaagt ccatttctgg tccctgcagt ctgaattttg
    17161 ctccttttaa tgtctgtccg ttgtcttctt ttttgtgtac cgttagatca aacgctttta
    17221 gtcgattttg atgagtgagc gtccatacat tatttgtcat ctcggtattt tttcccgtgt
    17281 aaatgatttt tccatattta tcgatttgga attcataatt tcccggcgaa gtatcatatc
    17341 cggcaggacc agctgtttca acgactcgat atttaccagg tgtgagtgaa tcaaaactgg
    17401 caagaccttt ttcattcgtt gtttggctgt caattggctg ataagtacca tttgcttctt
    17461 ttttctgtag ggaaaatgct gctcctttaa gatttatctc tttgtccgtc ccttgcatcg
    17521 catacttacg tactgctaca ggaacttcaa caaaaggatt ttcaatagtc aactggatcg
    17581 tatcatcgga agtagtgact gtttttccat caatggttac cgtaccatca gaagcgatct
    17641 tgatttccca agtagtcttt ttgcttaatc catgcccttc tggagctttt gtttctgtca
    17701 gcgtataggt catttctttt tgcaatttga cattttctgg aagagaatag gttccgtcgc
    17761 catgatctgt tagtaaagta tcaatagaat ctcctgttaa tttgaaaaca gcgccaataa
    17821 gatccttttc acctgtagag gaatttttcg ttattttcaa gtttaacggt gtgaattgtt
    17881 tagtattttt ccatgtcatt gcatctattt gttgagaatc gtatcctggt acaggtaatt
    17941 ctttgattgt ttggtaactg aatgcttgac cttgattatt gtattgaggt agtgataata
    18001 tctcttgata actttctccg ctatttgcag ataatttgtc aatgtctgca cgttcccacg
    18061 tatttgttgt atctttagct ggtttaatga ttcgaatata tccgtttttc caagctgcag
    18121 catttgtcgt atgttcccgt tgaatctcaa aagtaacttg atctggacga tcagctagat
    18181 tgttgtcaaa ttcttcccat aactttttga tgtgaagact gactccggga gctttagcag
    18241 atggtatacc gaattcagct aattcattgg tatcaatgtt tggctggaaa gttgtcctgc
    18301 cgttcatttg ataccagaaa tttggatgga agtcctcatt ttctgtttgg attctcactt
    18361 ggtaatggat ttggatttct tggtcttttc ctaaatagat ctgattgctc ttgatggtat
    18421 ttccttctat ggaaatagat ggagtgaccg ttgtaggact tgtccccaca ctcttgactg
    18481 aaagtgtacc aggctgataa atgaatggtt ctgcgattgg atcatttatt tgtccattgc
    18541 ttacagttgc tgagatctgt acagcttttt tggctaggta ttcagagata tctgttgcat
    18601 gatcagctga ttcatagtaa agatcgcctt tttcatctga tgaaaccatt tgtcgcatac
    18661 gagactctac ttctgctttt gagagaccag ctgccggatc gctttgaagt tggatgccaa
    18721 gaccatgtat ttcgattcct cgttctttga gtgccatcgc ttctccgatg gttgcgataa
    18781 acgtactgtc gattcttctg gatagattgt tttggtcagg tgcatcatag attcttgaga
    18841 ttagagaagt atttcccggc cgatcttgcg tattagaaaa ctgagttccg taataattgc
    18901 tgcttgattg tgcgtgtacc cgctgtactt tataggaaaa tgttggtaca ccatccgtca
    18961 gcaaaacgat cactttttta tgtccatttg gaacggatag catgcttcct gcatctctta
    19021 gtgctttttg tgtaaaagta ccaccatttg tccgtgaagg tgtaatggat tttacttgat
    19081 ttttcactga atcaaatgaa cccatctgta ctgcaccgtt actgtagcta tatccttcgc
    19141 ttgagtagcc gacatatccc atattgattt tgtctgtgat accgctatct gctaaagtat
    19201 cgacaaaacg atcgacacca atctttactt caccgatccg attattgtcg ttcatacttc
    19261 ctgaccagtc tacgaccaat acgagatcaa gaggcgtgat atctttttgt acatttccac
    19321 gagcattcaa ataaacatca aacaatccgg gtgtacttgt ttcctttgca tactttcgta
    19381 tcgcatagtc tgcttgatta ccggttcctc catattcgat atacgaatgg gtccgatcat
    19441 taggatctcc attccaagaa tttataccat cccattgatt tgttccgttt ttattgcctt
    19501 gatgatcaag aacatttgta tttcctgtag gttgccaact ggctttagga taagtgccct
    19561 gatcatctgt tgtatattct gtaggaatcg tttgattaga tggcacactc acagctggca
    19621 aggataatgt aggcaatgta aatgggctga cagaattttc gttttctttt gatgattctt
    19681 cttctatttc gtctggcttt ggtactgaca catctgtatc tttcttattt tctgtctcat
    19741 caataatgtc atattcttta ttatcgacga tcagcttgtt tgcctggctg tggtaaagtg
    19801 ctggatcgat tggaaaactc agatcgatag tttgtttagt gtttgatgag atttccaacg
    19861 taattttccc gctatttacc tgataagcat agccatctcc ttcttttctt tccacatcac
    19921 ttaagtaatt ttcgactaga ccaatttctt tttctatctt ttcagcactg gtgttattcg
    19981 catttagctt gatgttcagt gctttttttc cagactgatc cgaaatgact tcagctgaaa
    20041 ttgtttctaa aactgttgga tcatctgtag tgtctgctat tgcagataat ggcacaaagt
    20101 ttgataaact caatattatc agcatgaaaa taactttcag tttcttccct gtggtcgtca
    20161 tttttcccct cctttttctt tttttctgtt ttgacgtgaa tagtgaaaat tattaaaata
    20221 aacaggcgtt acataatttt atttatttta atatgataat tattatacga agggcgctaa
    20281 agggaatcac accaattttc ataccaattg gtttcattta tgaagttgat tccttcgatt
    20341 aatgagcgtt tcattgaaag aaaagggttg agggagaaaa acatatgatc aggttgttgg
    20401 aaggaaacta tagaaagatg gaaaaaatca ttcgaattct gtccatttct gacaagtgga
    20461 tgactaaaaa agaacttgcc aaacgaattg gtagttcaga atctacattt gtaaggtata
    20521 tcgaagagat caaaatccgc tggggaacgg atgtaacgat tcaatcttca caaaaattgg
    20581 ggtatcgtct tgaacatttg aatgtaccag cttatttaaa tgttctgatg gaaatgtcgc
    20641 aaagttctac aaacagtcgt ttattaaatg agattattgt caatccgggt aaaacgattg
    20701 agtattattg tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag ttgaaacaat
    20761 gcaatcaaat tttagaaagc tatttattaa aagtgatcgt tgaccaaggt tttcagttaa
    20821 ctagccttaa aagtgagctc cagttgcgta tctttgttac ctttttcttt ttaagttact
    20881 acggaagacg cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa attatggtca
    20941 agaataattg tcagatgaat ttgtttcagg aagaaagccc atatgagcaa tctttcttta
    21001 ttatgtattt tgttgtgcat ctcatgagag aatcccaagg aactccattt gaatggaaag
    21061 ctggagactc ttatgtacag ttgataaata tcgataaaaa agacttctta atcttgaaaa
    21121 atctcttagg aaaattgaag tacagcagct atataaaagt agtgaactat tttgtcaata
    21181 cgattttttc agaattttcg gagaaagaaa aaaatgaaat cagtgagcag atacgaaaaa
    21241 atatgtccta ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa gaatatgttg
    21301 ttcaattatt aacgcaaatt tattttttct cccgtatttt cccatacgat acgactcatt
    21361 tgacgatccg tgcaacttac tttgcacagc agatgcaaca ccgccagata cgtctatatg
    21421 ctggatttcg tgcagaactt caattttatt ccactctact gaatcagaat ctgatttatt
    21481 atttatctag ctatatcttt tggatgctta tcggtaatcc tgaaattcac cattatactt
    21541 ctgataaaaa aatactgatt atcagcgact taagtcttcg acatagtcaa tatattgaag
    21601 aatatatcag tgatattctt gctgtgcata aaattcactc tgagacaact gctattactg
    21661 aagatcagct gtcgaaatac aatcttctcg agtatgatct gatcgttacg aatcaaccca
    21721 ttttgaatgc ccatgttcct catatattga ttgatgatag tgtgtcattc gcaaatgaag
    21781 aagaactcat ccgcttattc gagttatgaa taatcaaaaa acagctatgt ataaaccacc
    21841 ctgataaaaa tttcaggaca aatacacata gctgtttctt tttattgtat tccttcagca
    21901 atccaacgag atttatagcg aaagtctttt aacaactctt cttttgtcca attatttgca
    21961 atgagatccg tcaaagtgtt cattacgagg gtcatggaaa agtataggaa actaactgct
    22021 tcttccgacg attgaatgga gaaatgattg attcttaata attccagcca tttttcgttc
    22081 attggagaat cagccgatag ttctctacgt ttggttgtct gctgatcatt tcgctgggtt
    22141 aacaagtata acctttgcca atagatactt tcgtgatcta agtcgcagca ccactctaaa
    22201 tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt taagatatct
    22261 tgatggacta tgtgagaact gcttttgatc gtatacaaat aagcatcttc caaatcttga
    22321 aaatatttat aaaaagcacc gcgcgacatc cccatcttgt caacgatttc tgagactttg
    22381 acttggctga tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat tctctgtttc
    22441 tttgcgttcg ataaattgaa aaaagtttct tttggcatcc acatctctcc tttatctctg
    22501 attatacgtg gatagccctt cattgtcatg aaggattcat tccttctttt tctcttcttc
    22561 tgtcaagtac ccatcttcca ttcgataaac gcgatcactc cattgaatca tacggacatc
    22621 atgagtaacc ataatagtcg ctttttgttt ctcatgagct tctttaacta gtaatttgac
    22681 gacttcatat gcatgatctg tatccaaact agctgttggt tcgtctgcta agatcaagct
    22741 tggctcatta tataaagcgc gagcaattgc cactctctgt ctttctccac cagaaaggtc
    22801 tttaggataa ctatttttaa gtgactggat atctaaagat tgcagcaagg aatcgatttt
    22861 tgttgaatct tctttttttg aagtaacttg gtcaactagt tcaaattgtt cctttaccgt
    22921 taaaaaagga atcaaattag agctttggag aataaatcct atttctttaa agcgtaaatc
    22981 tgctcgtttt ttttctggaa gctcagttag tgagtgaccg ttgatcacta tttctccgga
    23041 agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta cttttacctg aaccagaagg
    23101 accaatgatg ctgacgaatt ctccttcctt tacataaaaa tcaattgctt tcaaagctga
    23161 gacctttgta tgaccagaac caaatgtttt ggtaactttt ttcatcgtta atattttttt
    23221 catttttatt aacctcctat tgctgtaatt gggtctactt tggttatcgt acgtatcgaa
    23281 aacaatccgc ctagcattgc gacaatgatc aaaatcccac tatatagcaa ccattgtgac
    23341 caaaaaacag caaaaggcat cgcgcttggt aaaatcagac tagtcaaata agcaaacaaa
    23401 agagcaagca gaacgcctac caccccaaca ataaaggact gtgctactaa agatttagcg
    23461 atgaagaaat ttttgatccc ttgtgctttc attactccaa aaatagcagt cttctgaagt
    23521 gtcatcacgt acatgaaaat accgaccacg gcagcaacca ctaagaacaa aaagtatatc
    23581 atcgcattta aggtcatatt ttgggcagaa tagccaggaa tactttcaat gaatgtccca
    23641 atagttaata actgtagacc cttcgatgct ttttctttcg aaattttagc tgaatccttt
    23701 attacgatcc cattgatcgg ctgttcttct tcagaagcaa acggctgttg tccatacttc
    23761 aaagcagtcc acgtatctag ggacgcatag atgacgggag aaactgtgta ataagtttca
    23821 ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc caattttgat cgtatctcca
    23881 attttatagc ctgaatcagc cagattttga gagatgatga tttcattctt tgttttaaat
    23941 tcatgccctt tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc gaaaactgta
    24001 acattttctt tgttttttcc tttgattgct ccgctgtaca atccgattgg tgctttctct
    24061 cctccttcaa tgtatttcag atctcctctt gttaattgtg aagctgcaaa tacttgattt
    24121 gcatcctccg acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt tttaaactct
    24181 tctgaaagtc cattcgctag tcctgacaac atgaatacga catacgcaat caagaacata
    24241 ataccgacga tcaagccata tcgtaattta gaatagcgaa tttctttgat agctagaaac
    24301 atttgttttt cctccctatg atagattgat agtgacaaaa tgtcacttgt tgaatagtgt
    24361 aactctaaag tgacaatttg tcacttgtgt tgtttttttc ttatcaataa attgtctaag
    24421 acatctttcg ataaaacctt aacatgagtc ataaaaaatt tatttaagcc ttatttatgc
    24481 tttccatgat aaactaaagt gaccaaatca atgaggtggg aaaatgatta aactattagt
    24541 agtagaagac gataaagtat tatccaataa tataaaagaa atactcgctg caatcggcga
    24601 gataacacaa gtttatgatg gtagtgaagg actgttcgag ttggaaagtg gagtttatga
    24661 tcttgcaatc ttggatttga tgatgccgga aatgaatgga tatgaagtgt tagcaaaaat
    24721 acggaaagaa aaaatacaga ctccagtatt gattttgact gcaaaagata gtttggaaga
    24781 taagattgaa ggatttgaac gaggtgcaga tgattatcta acaaaacctt tctatcgaga
    24841 agaactgatc atgcgcgtga aagcattatt gaaacgctcg ctaggtctct ttgatgaaaa
    24901 tgttctgaag tataaggata tcagttgtga tttaaaaagc aatgaagtct tgtataaaga
    24961 acaagttttg cccatacaag gaaaagagtt tgaattgctc gtttattttt tacaaaataa
    25021 gggaatcatt ttgacaaaag agcagatatt tgaccggatc tggggatttg attctgatac
    25081 gacgattaca gttattgaag tttatatgag caacttacga aaacatctgc gaagtactgg
    25141 attggataaa gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa gggtgtgatg
    25201 caatgaatgg agatattaca cgaaagcaac gtagtcgctt ttttgcgaca aatgtagcag
    25261 catttgcttt tatctttttt cttttgggat tcattacttt acagatatta aaccaatctg
    25321 catacagaga aacagatctg tcattagaga aaataaattt tgattctcga acagtacaat
    25381 tagagataca aagatatcag caaggcgatc cctttttaaa cccgactcaa acggaacaaa
    25441 acagaacaaa catggaacag ccattaatgg aaccatcaaa tgaccgtttt aacactcaag
    25501 tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc agctattggt gggcgtttca
    25561 accaaatcaa agaattaaaa atggatacga aaaatctgaa taccatccaa aatattgaat
    25621 taaatgaaga cagtggctca tctgccctct ctttccgttc gattacgaaa aaagcggaga
    25681 cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa atcaagaatt
    25741 cattagcgac ttttcgcacg attatgattt tgtgtatgat tgttttttgg attctctcta
    25801 ttggtatcag ttattacttg tctagtctaa gcatgcgccc tattattctt tcttggagaa
    25861 gacaaaaaga atttgttgag aatgcttctc atgaattgcg cacaccgtta acgattattc
    25921 aaaatagttt ggaacatctt tttaccaagc ctaatcatac tattattgaa gaatccgaaa
    25981 gcatcgctca agcattgtca gagacgaggc gcctcactgg attgacttcg gatttgctga
    26041 caatcgctcg gaatgattct gatcagcagc tgctttcaaa gcaaatgatc aatacacaag
    26101 aatatatcga acaacttgtt aaaccatttc aagagatggc catcatagat ggaaaagagt
    26161 ttattctcca taacgatgca acattagaag taccaattga tgaaaaaaaa atccatcaag
    26221 ttttggttat tttgctggat aatgcattaa aatatacgaa tccagaagat aagatcattt
    26281 tggattcatc tgttaaccat aaagaatggc tgattcacgt aaagaatact ggcccttcta
    26341 tcagtgaaga agatcaagca aggatttttg atcgatttta tcgggaagat caatctcggg
    26401 cgaaagagac tggcggatac gggcttggct tggcaattgc aaaacaggta atcgaacagc
    26461 atcatggaca gatttctgta tccaattatc ttccacaagg agtcgatttt aaagtccaat
    26521 tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac aaaaatcttg
    26581 tcacagccct tttttgttgc gaataaacgg tgttccagaa gtagcttctt cggaaataag
    26641 ccgaaatttc tttaagctga ccacttctgt cacaacctct ttagcttacg cttacgattt
    26701 tttattgcgg tcttcaatgt gtacatcacc gcttgtgtca atatccaatt cttctcggct
    26761 aacatcttct gtcacttttt caacatcttc atgttcttct ttatgaatcc caacttcttc
    26821 acgaataact gtatgtttgc ttacatctac ttgctcttct ttgattggga tagtaatcgt
    26881 gtcatcttcc aatttttcgt ctgttccctg tgaagattga tctgttactg gtttgcgttc
    26941 gatgacgatt tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt tgaccacatg
    27001 tttgtggatg tcgacttctc cagttgtcac atcatgtgta tttacatcta atttttcttc
    27061 ttttagttga attgtatcat catctagatt tggtttgcga tcgatgtctt cagtcttgct
    27121 tgtacccatt gtatcagtct cgccaggtac gtccatcgga tcagttcctg gtgtcatatt
    27181 caatggatcc gttgtatttt gaaagcctgt cgtgccacct acgtccattg gatctgttcc
    27241 agtgccagtt tctaatccag tccccattgt gcttgctggg ctaacagtat cttgtgtatc
    27301 cattgtatct tctgttgttt ctggctgacg atagcctttt actgtgatga caaattttcc
    27361 atcagaaata tctcctctat attgatataa aggatcattt tctggtgtag ccgtctcaga
    27421 atcataggta tcaaaagaaa aagtatcttt tattttttcc cataaagaac ggtcttcttc
    27481 accatccatc cgattttcat ctgtttcaac atctatacca gacaatccgt caaatcctag
    27541 tctagatgct gcttcatcag tagtataaat aacgatatca tctcgttcat acccttcgtt
    27601 acgcaaccgt tcaattactg ataatgcttc ttccctcgta ctatagcttc cgatgaccgt
    27661 taaatttttc tcttccatgt aaattcctcc tagcaaatag atttatgatt ggtatattgt
    27721 tacttataaa tttattctat aggagacgat tgttttctac aaatatttag attggaattt
    27781 gattaaaaca atttaattga aatttgaaaa catttatgtt aatgaaagtc atattctaat
    27841 aaaatgtcat ttgtttctat atgaattttt atgaaaaatt tcatataatt tccactcgtt
    27901 gtgatggttt aatttatcca atccatcaat aagcaaacaa cttatataaa aaaggattaa
    27961 ttctcaatat aattgctcct accatagaat aaatcttcat gtaaagtaat gaagtaccaa
    28021 ctatttctaa tacttcttta agaattatcc caacaatatc gttagacaaa ccattattga
    28081 ataaatggag agccaatcga ttctgaaaaa atgttttcta atttttcgga aataaaagaa
    28141 agagcctcct gccattagaa cgaaccaaat ccatttcatt gctcatgaag taaccacata
    28201 gctaaaaaga ggataaatca attaaaagat caaaagaatg actgcactaa taaaataact
    28261 aaaaatgtat cttcttactt catatctttt agctagtact tagttaaaaa tttgcttcct
    28321 aagatcaaga caaactgtct atcttcattt gatgaaatgc ggtaaaatat agaaaatgaa
    28381 cactattgga gggaatttgt aatggacagc aaaactagaa aacaactgca agaaaagtta
    28441 aagcaacgta cacacttgct cccttccgtc tttcaacagt ttttagaaga aaatcgttct
    28501 tccctttcat taggcagtcg ctatgaatac gcaaaagatt ttcatctgtt tactgattat
    28561 ttgagaaaag tattttctga agaagtggat gatcagctga ttatccattt agaaaaacaa
    28621 gattatcaga atttcttagc taaaatctat cgacatactc gttcgttcca aactacagcg
    28681 aaccaagaaa cggagcaatt gtttgaaaat agctattacg ggatttcaag gaagcaatat
    28741 tcattaaatc attttttacg atttttgtat caaaaaggac tgattgaaga agagatttct
    28801 cttttgggta caagtttacc ggaaacaacc aaagcagctc cgcgatatat cgatgcagaa
    28861 cttttcaaag attttgagca tttgtttatc ccgattgaag gctttcaaac aagcagagaa
    28921 gctagctttg aagaaaagaa tagaccgagg aaccttgcaa ttactgccat tcttctgtat
    28981 tgcggattaa agatccatga agtagtagaa cttaaacgta aagatgtatc tttgaccaag
    29041 cagattctta atctgaatag aaaagaaaac agactgaata aagtaccaat accaaaagaa
    29101 gcgatgccat ttctggagaa ctattttaag ttaacagcac aaaatacgga cgaaaattct
    29161 tttgtttttc gttcctcaca tgatcagcaa atttctcctc ggactattcg ccaaattttg
    29221 agcaaaatta cagtatataa aaacgtatcg ccagaactat gcagaaaaac atatcgttat
    29281 tatgcagaac tatatttaga tgatgctgac gcggtaaatt atttatgtgg taatcgatac
    29341 ctctcatccc attcttttca agatatctgg gaaaagatgg ttgatttttc ttatcacgaa
    29401 ttagcggcat tcgttcgggt ttcttccata taaaacaaat gtatgatctt tttttcacaa
    29461 taaactatgt tattatataa aggattatgt tacgattatc ttgaatttgt atattactta
    29521 acaaaagata atggagtgtg aatgatggag accataacgt attccccttt gaacttattt
    29581 acgaattttc aagaagctgc agctaacttt tccgagaccc cgattatctt tgatgagcca
    29641 ctttcagctt tcccagaatt aggacttgaa acaacttaca aaaaatgctg cgaaagtgtg
    29701 ttgaaacggg cttatcaatt ggctcatctt ggagtgaaat ccggagacaa agttattatt
    29761 tataagagtt ccgcttttga cacatacctt ctagctgttt ctgtgtctta ccttggtgca
    29821 gtgcctgtca tgacttctta tcatctacca acaacaacaa tggaagtatt tatcgatcgt
    29881 ttagaagacc ctttcatttt atttgatgat gagacaaaaa agcgagtgag agaaatttca
    29941 aatggaacaa aatcaaaaca gattcctatt ctttatttaa tggaacaacc tgctttgtct
    30001 gtttctcaat catttttaga caaaaatgaa atttcttata tgacccatac ttctggtaca
    30061 actggtatcc ctaaacttat ttgtcattct gctcattcta tgggatggcg gacgaaatgg
    30121 caaaaaacag ttttcacaaa aatttcggaa aagaaattaa tcgcttttca catctctcca
    30181 gtgcattcca gattcaatat tggtatctct tctttgatgt ccatgggatt tcctatgatg
    30241 cctttagcaa acgcacaaag ttcaaaagtt gttcatatgt tggaagctca ccggccaatt
    30301 gcactagaga cccatccgaa taactttgtt cagtggcgtt ttactgctaa ggagcatcca
    30361 gaagcttttt cgggtatccg atactatcac tccacttttg atgctataaa caaccagaca
    30421 atgaagacat ttttgcgaac gtcgatggca caggatgcgg tatttcttca agtatatggc
    30481 caaagtgaat gcggaccaat gattcttaag gctcatacac tagagagttt gaaaacaagt
    30541 gatgcaagag atatgggcgt tggactggaa gacatgacaa gcgctcggat tacagattca
    30601 gttgggaatg tcctacctga aaacacagat ggtcatatac aattactgtc taaaggacgg
    30661 gctttgactt attacaagga agatgcacgg ttccaggaaa atgtttatgg cgattggtgg
    30721 gatagcggag attacggttt tatggataag catggccacc tatttttgaa agatcggcaa
    30781 gtggatttga tcgaaacgat caatagtaca ttagctattg aagacttttt attagattcg
    30841 ttagattttt tggcagaagt agtgattgta agggataaaa atcattctcc acaaccgatt
    30901 attgctttag ctcctgataa agaaatggat tggaatcgat ggtgggagca agtccatgaa
    30961 cttcctcgct tgaacgtccc tatcatccgt gattttgatg caatcccgcg aacagctaca
    31021 atgaaagtgc aacggcttca aatcgagaaa gaattaaaat cacaaaagtc ctaactattt
    31081 aaggaaagaa agatccattt tggtctttct tttctttttg tttttttaaa ttggatgaga
    31141 taaaaatatg ccaattggct gtttttcttt tttgaaaatt tcgttatact cttattcatg
    31201 gaggaaaatg ttatgaaaag aatcttgaca attggcggtt ctgatccatt tgctggtgga
    31261 ggaatccaaa cagatttaaa aacttttgag aattttggtc tattcggact aagcgccttg
    31321 acttctatag gggcactaga tattaatgag cgatttatgc tggaatctat atctttggaa
    31381 ttgcttaaac gacagttagt ttcgattgat aaaatgactt cattagatgg tatcaaaatt
    31441 gggttattga attcaagtga agcaatcatg gttgttcgag acttcataaa aacaaaacaa
    31501 aatatcccaa tcattttaga ccctgttttg gcattcaaag aaactgaagc tgtgatcaat
    31561 caattgtata tttcgcagct catcgaagaa ctttttccac ttgttacgat gattacgcct
    31621 aatttggcag aagctgcttt actttctggt caagctcttc cctcttcgtt aacagaaatg
    31681 atccatctcg ctaaagaact cactgcttca ggaacaaaaa acattgtgat caaaggcggc
    31741 acaaggatgg ctggagatga agctgtggat cttctctatt caattgatca ttatgaggta
    31801 ttttctcata aaaaaatcga cactacaaca gtaaacgggg ccggttgtgc cttttcttct
    31861 gcaataacag cgaatcttgt gttaggggca gattttacca aagcagtagc agaaagtaaa
    31921 aaatttgttg cccattgtat ttctaatggc gtgctgatga acgatcatac cggaagtgtc
    31981 tggtctggcg gtattcaaaa aggaggaatg agaaatgaaa actaagcaat taacgatcta
    32041 tgctatgctg attgcgttga cagttgccct ttcattaaca attttgatcc ctgtaccggc
    32101 tacgaatgga tttgtgacat tatgcgaggc cgggatctat accgctgctt ttctttatgg
    32161 tccaacgggt ggattagttg ttggtgcttc cagtggtcta ttgatcgatt tgatttcagg
    32221 atatccgcag tgggcagttt tttcattagt gatccacggg ttacaaggat atgttgctgg
    32281 ttctttcagc aaatcttcta ctcgtatgtg gggattaggg cttttattag gaagcttagt
    32341 tatgatcatc ggctatggta tcgccggttg gattttatat gattggccgg ctggagtggc
    32401 ttctatacca ggaaacatct tccaaaatct gattggaatg ggacttgcct ttcctataac
    32461 tgcaagtctt caacgtattc aagcacggaa gcaatattaa ttgaaaaaga aggttttaga
    32521 aaatgaagtt gtcagaaaaa gaattaaaag accaattgac tgaaatagtt aatgacatat
    32581 tggctgaggc acacttgaaa aaaggtgatc tttttgtttt aggatgcagc acaagcgaag
    32641 tcgttggagg acatattgga aaaaattcca gtgcggaagt tggtcaatgg atcatccgta
    32701 cgttaaaaga gttgttggat cctaaagaaa ttgcgctagc tgtccaaggt tgtgaacatt
    32761 tgaaccgagc attagtagta gaacgaacag tggcagaagc aaaaaatttc gaaattgtct
    32821 ctgttgttcc tgcactacat gctggaggtg cttgttctgt tgctgctttt gaccagttca
    32881 atgatcctgt cgaagtggaa cacgtagttg gacaagccgg tatcgatatc ggtgacacat
    32941 caatcggtat gcatatcaag catgtccaag tacctgtcag acctcgtttg aaaacccttg
    33001 gacaagctca cgtgacagcc cttcgctctc gtcctaaata tatcggtggt cctagagcga
    33061 attattgaaa tgagtaattg acagagcaat ctgaaaaact tcctcgcaaa gtccaactaa
    33121 tgaactttaa cgaggaagtt ttacttgttt gttttatata accgagcttc ataaggtgct
    33181 agtatgcctt tagcttcaaa ttctccgcta gaggaatttg acaagagtaa tgtccaggac
    33241 tgatctgctt cttttggaag ttcatagtca gcaagtgatt ctgttaagtt taccataata
    33301 agatattgtt catcttctaa atatctttca tagacataaa gttgtggatg ttcatgaaga
    33361 tattctttat aagaaccgta tatcagacct ttattggttt gtctaatgcg gatcatgttc
    33421 ttatagaatt gcaggaccga ctctggttca tattcttgtt gttctacatt gatcgttttg
    33481 gtatttggat tagtgatcag ccaaggttca tgtgtagaaa aaccagcata ttgttccgaa
    33541 gtccattgca tgggtgtcct gctgttgtct ctcgttgttt cacgaatgat atccaatgct
    33601 tcttctcttg tcttaccttc cgcaagcagc cttttataaa gtcttttcga atctactgca
    33661 tcaacttgat cgatcgattc aaagggcata ttcgtcattc cgatttcctg tccttgataa
    33721 ataaatggcg taccttgcaa aaagaaatac atcatagcga ttgcttttgc agacatagcc
    33781 caaaaatcgg gacgagtatc tccaaacact gaaacggcac gaggaacatc atggttttcc
    33841 atatataaag cgttccatcc gataccatcc aatgaagttt gccaagcgct taatgcatgt
    33901 ttcaatttaa ggacatctaa ttgcccttct ttttcttgct gccagatatg gatatgatca
    33961 aattcgaata tcatatcgaa ataaccgtct ttccctaccc attgtggtcc ttcttctgca
    34021 gtgacaccac ttgcttctcc gacagtcatg atatcgtact cttcaaaaac atgtttcaaa
    34081 tcggtcaaat gttcttcgat tcctgagaca ttttgaaagg gtgagaaagg gttctctgtg
    34141 gcttttactg aatattcatc tttttttaca tgagaaatcg catctaaacg aaatccatct
    34201 atccctttat ccaaccacca tcggatcata ttgaatagtt cttctttcaa tttctcgctt
    34261 tcccaattta agtctggttg ttcttttgca aaaacatgca gataatactg tccagtttca
    34321 tgagaatact cccaagcaga accgccgaaa atcgatgtcc aattattcgg ttctttgcca
    34381 tctaccccgt cgacccaaat gtaataatcg cgatacgggc tttctttaga ttttttcgat
    34441 tcttcaaacc attgatgctg atccgaacta tggttgatga caagatccat aatcactttg
    34501 atccctaatt catgtgcttc ttttagcaat aaatcaaaat cttccatcgt tccgaatctc
    34561 tcttctatat cttgatagtc acttacgtca taaccattat cgacaaatgg agattgatag
    34621 atcggattga tccagataaa gccgatacca agatctttca aataattaag tttttcacgg
    34681 atcccattga tgtctccttg accatcgcca ttgctatctt tgaaactagc aggatagatt
    34741 tgatagccta ctgcttcttt ccaccatttt tgttcttgta tcatatcgtt tcactccttt
    34801 aacggtccct cttgattata accgattcta ttttagaagt atccttgtta ccggtaacta
    34861 gaaacgaaaa gctcaacgaa tctgctaagt gcgttcgttg agctttattt tatcatagat
    34921 agcgggcaat cagttgaatc atttgcttgg gaaattcatt caagtcagta atatctacaa
    34981 aacgactttc accgtagatc gacttgattt cttctttgtc ttggccaata gctgcggaga
    35041 caaataggat tccttgacgt tcatattctt tcaaaacaga ctggatatct tcttttgctt
    35101 ttttgcctgt ataatccggc aatgcttttg gctgaccgtc actaatatta agcaacagtt
    35161 tggttgttgc ggattgtctg tttaattttt ctgctgctaa acgaagtgca gcaccatcac
    35221 gattgttttg ccttggtttc atagtgacta atttttcatt taaccattga aatccgtctt
    35281 caaattcttt ataagaaaac aaagaagtct tttctcttga cgaacggtct gctgtatctc
    35341 cgtaaatcaa caatgggata tcgactttct tagaaaattc ggcaatggca atcgctgctt
    35401 tttttgccgc ttcgatccga tcatcccgta tcattgaccc agattcatcg atccgtacag
    35461 ctaccgctaa agaaggttgc tcatgtggcg ggttcttttt gtcaaaattt ctcaaatcac
    35521 catatgctac tttgcttgca ttgaaccgct gaccttcgta ttttcctttt tggtacgtct
    35581 ctgtttgttc attatccagt aactctaaaa tttgccggct aagtgattct actattggca
    35641 ttacttgcat gtgtagctca cgtgcttccg gcgtttttcc ttggaaagct ggtcggtgaa
    35701 cgatgagacc ttctttttca tgaatcgttt ccttcatgat ttgtcgcgct tctttattca
    35761 gttgcttctt gatttcctta tcatgtgctt ccatgtcaga agcagacatt tcttgagaat
    35821 catctcctgt ttgttgttct ttttgatttt ttccttcgat atcgctactg cgttcttttt
    35881 gcatttgagg aggaatctcg tttttttcta ttgtttcttc ttgtttctct tctccctcat
    35941 ttggttcttc agcagacaac gatttctttt catctaagcg cagaatttct tcagcatttt
    36001 cagtgatgtt tcgttcattt gcttcttcgg cgctaatggt tctttctttt gtatgctcag
    36061 attgaggatg accccatttt tcttttctca atatgtcctg aagttccagt agcaatccac
    36121 tgtcagacaa agcttgttca agaattgcca attcatctga atcagtggat aatttataca
    36181 aaacttttgg ataaatcgac tggagtaaat cttgtccttg cttataggta tttgcccaat
    36241 aaaaataaga acgcattcct gctacacctt tgatggcgtt agcttttgca gtttcatcca
    36301 acaaacggat aatctctgcc atctttaaga gaagttcttg tttttggatc atagttttcg
    36361 ccatcgcacg ttctgccatg acttctaagg caggaagatc cattttttcc gcatgttgca
    36421 tccgatctct taaagattca tttaatgggc gatttccttg gtaatttcga tttgtagtga
    36481 tcacaatgac gcaatctgga tggcgacgaa tgattcctgt cggtaaattc aacatcccat
    36541 ttggttcgag cgctgagtta agtgccacaa gaacagaggc atctcggatg acagtcggct
    36601 cttgaatctc taataaatag cctttttcaa tcgctcgaac gatttcagaa gggtaatagc
    36661 tgtaatggat tgcatctgga taatcagctt cgatcctttg aactagctct gctaattttt
    36721 ctttagcaga aaaaggatca atgccgtaat ggttctggat cagtgtcagt acagcttcaa
    36781 gtgtatctgt ttggtaaata gcttctagta attcttggtc ttgttcctta ttagaatcta
    36841 ctacaggtaa taaggctccg aaaacatctg atttgtccat atctgcaaaa caggtcactt
    36901 ttgtataggg aagttgcaga tcagcagata gtgctttggc taattgtgtc ttccccgacc
    36961 cggcatctcc ttccaaaagt acgttactga ttttcatttc aggatcttgc caattccctt
    37021 tgatttcgga ggcgatccgc cattcttctg cacttgtccg atggcttttc ggtttcttcc
    37081 agatcatttg cttttctagc cgtgaaaagg ttcggttttc tgttagtgag aaattctcca
    37141 tgcttctctt ccccttttga tcatctctgt ctattaatat cctagctcat ctaaaagacg
    37201 ttttaaaata cggaatcgtt ccccaatcag ttctccgtct ttttgtagtg tatcgtaata
    37261 caaaatgatg tcttcatcga tctttggatt tttggtatct acttctaccg tcattcggtt
    37321 cccttgtaat ccaatgatgt ctacgactgg attttctgga tcataaaatt tttcataacg
    37381 ataagcatct tggaagtata aacttccttg cgcagctaag atcgctaaat ctaaaatacg
    37441 tgtgattggt aattcttccg actgtcgtga ccatttttct ccagtatgtc gccatacctt
    37501 gcctgagata tcgacttttc cacgatcatt ccactgagca aggcccagtg aaagcccttt
    37561 tgcatccata tttaatgcat ttcttccatc aactttatca tagtcttcta caacgattac
    37621 tggtttatgt tttaaatttg ttggtatttc cattatttta actcctcatc cttttatttt
    37681 tagtaaataa gtaaattagt agtttactca tttactaaac tatatgcgac attatagagg
    37741 attgaagtga ggattgcaaa gaaaaagagc ttcattgata gaaataaaaa caccatctcg
    37801 ttcttttaca cgctcattag taacatgtag aaagtccaag atgatgttgg aatattt
//
LOCUS       DARBFD010000015        35633 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000015 DARBFD010000000
VERSION     DARBFD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.015"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            119..1189
                     /locus_tag="SCK08_000778"
     CDS             119..1189
                     /locus_tag="SCK08_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200690.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1204..1872
                     /locus_tag="SCK08_000779"
     CDS             1204..1872
                     /locus_tag="SCK08_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1200691.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1892..2470
                     /locus_tag="SCK08_000780"
     CDS             1892..2470
                     /locus_tag="SCK08_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1200692.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2873..3784
                     /locus_tag="SCK08_000781"
     CDS             2873..3784
                     /locus_tag="SCK08_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1200693.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4040..4957)
                     /locus_tag="SCK08_000782"
     CDS             complement(4040..4957)
                     /locus_tag="SCK08_000782"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEG1200694.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5159..5596
                     /gene="zapA"
                     /locus_tag="SCK08_000783"
     CDS             5159..5596
                     /gene="zapA"
                     /locus_tag="SCK08_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEG1200695.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5654..6201
                     /locus_tag="SCK08_000784"
                     /pseudo
     CDS             5654..6201
                     /locus_tag="SCK08_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            6316..8676
                     /locus_tag="SCK08_000785"
     CDS             6316..8676
                     /locus_tag="SCK08_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1200696.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8763..9077
                     /gene="trxA"
                     /locus_tag="SCK08_000786"
     CDS             8763..9077
                     /gene="trxA"
                     /locus_tag="SCK08_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEG1200697.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9259..11037
                     /gene="uvrC"
                     /locus_tag="SCK08_000787"
     CDS             9259..11037
                     /gene="uvrC"
                     /locus_tag="SCK08_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEG1200698.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11182..12081
                     /locus_tag="SCK08_000788"
     CDS             11182..12081
                     /locus_tag="SCK08_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1200699.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12074..13336
                     /locus_tag="SCK08_000789"
     CDS             12074..13336
                     /locus_tag="SCK08_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200700.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13499..15397
                     /locus_tag="SCK08_000790"
     CDS             13499..15397
                     /locus_tag="SCK08_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEG1200701.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(15696..16415)
                     /locus_tag="SCK08_000791"
     CDS             complement(15696..16415)
                     /locus_tag="SCK08_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEG1200702.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            16752..17084
                     /locus_tag="SCK08_000792"
     CDS             16752..17084
                     /locus_tag="SCK08_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200703.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17071..19074
                     /locus_tag="SCK08_000793"
     CDS             17071..19074
                     /locus_tag="SCK08_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEG1200704.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19093..19563
                     /locus_tag="SCK08_000794"
     CDS             19093..19563
                     /locus_tag="SCK08_000794"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEG1200705.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19584..20168
                     /locus_tag="SCK08_000795"
     CDS             19584..20168
                     /locus_tag="SCK08_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEG1200706.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20183..21169
                     /locus_tag="SCK08_000796"
     CDS             20183..21169
                     /locus_tag="SCK08_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEG1200707.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            21159..21470
                     /locus_tag="SCK08_000797"
     CDS             21159..21470
                     /locus_tag="SCK08_000797"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEG1200708.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21492..23273
                     /locus_tag="SCK08_000798"
     CDS             21492..23273
                     /locus_tag="SCK08_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEG1200709.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23266..24642
                     /locus_tag="SCK08_000799"
     CDS             23266..24642
                     /locus_tag="SCK08_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEG1200710.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24649..25284
                     /locus_tag="SCK08_000800"
     CDS             24649..25284
                     /locus_tag="SCK08_000800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEG1200711.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25314..26669
                     /locus_tag="SCK08_000801"
     CDS             25314..26669
                     /locus_tag="SCK08_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEG1200712.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26861..28255)
                     /locus_tag="SCK08_000802"
     CDS             complement(26861..28255)
                     /locus_tag="SCK08_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1200713.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28347..29318
                     /locus_tag="SCK08_000803"
     CDS             28347..29318
                     /locus_tag="SCK08_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEG1200714.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29315..30097
                     /locus_tag="SCK08_000804"
     CDS             29315..30097
                     /locus_tag="SCK08_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEG1200715.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30266..30637)
                     /locus_tag="SCK08_000805"
     CDS             complement(30266..30637)
                     /locus_tag="SCK08_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1200716.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30771..31214
                     /locus_tag="SCK08_000806"
     CDS             30771..31214
                     /locus_tag="SCK08_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEG1200717.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31257..32066)
                     /gene="yidA"
                     /locus_tag="SCK08_000807"
     CDS             complement(31257..32066)
                     /gene="yidA"
                     /locus_tag="SCK08_000807"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEG1200718.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(32085..33452)
                     /locus_tag="SCK08_000808"
     CDS             complement(32085..33452)
                     /locus_tag="SCK08_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1200719.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33436..34266)
                     /locus_tag="SCK08_000809"
     CDS             complement(33436..34266)
                     /locus_tag="SCK08_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEG1200720.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34429..34866
                     /locus_tag="SCK08_000810"
     CDS             34429..34866
                     /locus_tag="SCK08_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEG1200721.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            34942..35562
                     /locus_tag="SCK08_000811"
     CDS             34942..35562
                     /locus_tag="SCK08_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEG1200722.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        11645 a         6056 c         7660 g        10272 t
ORIGIN      
        1 agaataaaaa attcatataa tgaaatagcg gaagaatttt ttgagcataa tattcgccaa
       61 aaatttatca agtgataaaa ttgttgtata aaatgattat ttcaggagga ggagttaaat
      121 gtttttagca ttaaatgaaa ttatgcattc aaagttacgt tatgcgttag ttgcaggggt
      181 gatgtttttg atcgcctacc tggtattttt tttgactggg ttagcttatg gcttagctca
      241 agataatcga acagcagtcg acaagtggga ggcagattcg attgtgcttt cgaaagatgc
      301 aaattcaaat ctagggatgt ccatgattac taagaagata gcagaagaag tcgaaggtgg
      361 gaaagtggcg tatcttgccc agacacctgg agtcgtgact agtaaagaca gtacagaaga
      421 aggaaaaatc aatgttagct tcttcggtat cgataaaaat caattcatca tgcccaatct
      481 tgttgaagga aaagcttttg ataacgatga tgaggcagta ggcgatatta gtttgaaaga
      541 agaatatggt ctagccgtag gagatacagt taagttatct ggtagtgaca agacgtttaa
      601 actaactggc tttacggatc atgccaaatt caatgtctca ccagttctct atacaaccat
      661 caatgcttac caagagatcc gcttcgaaaa agaagataca agtgaaaatg cacggattaa
      721 cgcaatagtt gtcagaggta aaatcagtaa tttacctgaa gatttagaac agatcaaaat
      781 ttctaaattc atcaatgaat tacctggata taatgcgcaa gtattgactt ttggatttat
      841 gatcggattt ttgatcgtta ttgcggctat tgtgatcggg attttcattt atgtactgac
      901 gatgcaaaag attaatatct ttggtgtaat gaaagcacaa gggatcacag gaggatttat
      961 tgcgcgttct gtagtggcac aaacattcat tttatcattt gttggtattc ttcttggtct
     1021 attaggaaca gtgggaacat cccttgttct acctgatgcg gttccattcc aaagcaattg
     1081 gctgtttttt ggcgtcatta gcttgttgat gctggtagta gctgtgttag gcgcactatt
     1141 ttctgttcgg acaatcgtaa aaatcgatcc gttgaaggca attggataag aaggaggaag
     1201 agcatgaaag caatagaatt taaagaagta gaaaaaagtt tccaagatgg cgaccaagta
     1261 gttaaagcat tgaaagaaac aaccatgagt atcgataaag gggaatttgt tgcaatcatc
     1321 ggtccttctg gttcaggtaa aagtactttt ttgactttgg caggcggatt gcagacgcct
     1381 agcagtggct ctgtatttat caatgatcaa gaattcagta atcaaaaaga aagtgaacgc
     1441 gttaaactac gttttaaaga aattggcttt attttgcaag cttccaatct ggttcctttt
     1501 ctgacagtaa aaaagcagtt attattagta gataagatca aacatgaaca tcgtcaaaaa
     1561 gaagcaaaag aattatttga gcagcttggc gtggataagt tgatcaacaa gtatccagaa
     1621 gaactatctg gtggagaacg gcaaagggtc gcaatcgctc gtgcattgta caatgatccg
     1681 tccattattc tagcagatga accaactgct agtttggatt cagaaaaggc acgagaagta
     1741 gtcgatatct tggcaaaaga atcaaaagac aaaaataaag caacgatcat ggttactcac
     1801 gatacacggc tgattgacaa atgcgataga atatttatga tggaagatgg tgtattgaaa
     1861 gaaaaagaat aaccaaaaag caggtggaaa aatggtccat cgatacaaca aagatacaca
     1921 gcaaaaaatt ttgaaagaag cgactgactt gtttatgtcc aagggatatc tcggcacttc
     1981 aacaagagag atcgcacaaa aagttgggat cacccaaccc aatctctatc attatttcgg
     2041 tgataaagaa aaactatata cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc
     2101 tttgagagaa atcgttcaaa caagcgaaac gggatttcaa gtaaccttga caaaaatggc
     2161 gcagtttttg attgagacac atctggtcga tttgtttctg atgcttcacg atcttgaaag
     2221 caatctttca aaggaaacaa gagatcatct tttctttcta tggaagaaaa attatcgtga
     2281 accttttgaa gaaatctttt cagcaaacca atctgtgcta agaaatggga tttcaaaaga
     2341 aatcgcagca aggcattttt tcttagtgtt agcaccttac atcactcggt cgaaaagacc
     2401 tgtggaagaa ccgctaaatg tggcgcaatt gatcgattta tatttgaatg gtgtattagg
     2461 agaagcgtaa agctgttata tagggagaaa aatagttagg tgaagaagca agatatgacg
     2521 aagatcgtta tatcttgttt tttttatata ggtagttttt cataaataca aatttgttat
     2581 tctctcatga ttatcagggt atatttttat ttattatgtc ggtgttccag gaagtgtttt
     2641 aatttaataa cgaagaaatt aattgtttgt agaatgaagg aaactattta ttgggtagat
     2701 gaatcaccac gaatagtcaa aaggagattt caggatatgc caaaaatgaa aaaaatcatt
     2761 ttagcagtaa tgttattttc tacggttata tcttttacag cccctttttt gcgtacttaa
     2821 gctgatttta agttttgacc cttagtctgg atctacgagg tgagagtcaa gaatgaaaaa
     2881 aataattgga gcttttgtat ttgcaatcat tatcattatt atctgttttc aaatagatgt
     2941 ttttggtcaa aaaaagatag ttggacaaac ggtcagcagt acgaacagta cggttacaaa
     3001 gacaaaggaa tcatttgatg atacagaaca agagacatta atcaaaatcg gaaaaaatga
     3061 ccgtacacta tatacagatg actcgctaac taccccactt gcaactataa acggcggaga
     3121 gctggcggag tttctttctg aaacaaaaaa tgcttatcag atcaagacaa atgatggcta
     3181 tactggatat ttagccctcg aagatggaac caaagtcaca aagaatatac aaacaaaacc
     3241 ggaagagcta tctgatgcag tgattgtatt agatcctggg catggcggaa atgatactgg
     3301 ggcattaagc aatgatgaac aaacagaaga aaaagatata acattaagta cagccataaa
     3361 agtaaaaaag gcgctagagg atgctggagc aacggtttat ttgacccata ctacagatga
     3421 attagtacag cttggagata tatgcgacta cagtgaagaa aaaaaggcag atgtctttat
     3481 cagtttacac gcagactcta cggaatatgc gaatgaagca actggaatca cgacatatta
     3541 ttactatgga caagaggaaa cattagcaca gacaattgct gactccttca ctgatctgcc
     3601 tctggattca agaggaatct ccactgggaa ctatcaagtt cttcgagaaa atcttcaacc
     3661 ttctatttta atcgagatgg gttatatgaa taatgatagt gatttaaagg aattagtaac
     3721 agacagttat cagcagcaga ttgctgcaag tttgacacaa gcattgacca cttattttca
     3781 gtaataaata agcccagcca aacaaaaata gctcctcata tttttcgttt ggccgggctt
     3841 attatcgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgag cctgccgttt
     3901 aactccgcgt cacaaggagc aatttcaaaa aacagcttct catgtttgat gactattgtg
     3961 acttgtgacc gaggagttgg catgagaaca ccgtttattc ggaacaaggg actgtgacat
     4021 gacttttgtc acagttccat tattttttta gcagcttcaa agctttttct gtattggcaa
     4081 aatgaagttt ggcatatttt tcaagcatag gcatgccgcc tttctccaat agttttgccg
     4141 ctacttggtc agatttcgtt ccagctcctg atggaagggt gattccagct tcagctgatg
     4201 ctttgtgtaa ctcttccaaa aagctggcac gactgatgat agaagcagca gcaacagcta
     4261 aatgatattg ttctccttta gtaacgaagt acagtttttc ttgtaattga tttttttcta
     4321 aacgcgcata ttttttaaaa tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg
     4381 gtttttctgg ctcgattttt tttagcagca aatggatcgc ttgattgtgc aaagcgactt
     4441 tcatccgaac ggcattgtat tttggttgga tctcattgta tttttctgga gataagacaa
     4501 gcagtttata ggggatggtt ttttttaaga tgtcagcaag ctggatgatt tggctatctt
     4561 ttaactcttt tgaatctcta acaccaagtt tacgtaattc tgcaagctga tcacgtttca
     4621 cataagcagc acatactgtt agcggaccaa aatagctgcc atttccgacc tcatcactac
     4681 ctaaaacgga taattgactg atattttttg gcaaagaaga ggctgttttt gctggcttgg
     4741 cttgtttatc ctgtgcctcc ccccatttat cagcttctat tgctgcattc gtaccttgaa
     4801 acatcacttt tccggatgta taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc
     4861 tagtataagg aatctgttta tttaacaaat aagagcgata gtatgctttc atttgactaa
     4921 gttggtcgtt tgttgctttg ataatgacgt tgctcataaa attctcctcg tttttataca
     4981 taaaactata ccgcaactca cgacttttcg ttttctaaga gattgcgaga catagcattt
     5041 ttttaaagtt tatcatataa tagttaagta tgtgaaaaat atttgtggag aacggtgaaa
     5101 gaaccaactt catggtacac taaataaaac tttcatcaaa taggaggaaa tgattcgtat
     5161 ggcgcacgaa aaaacacgct acaaagcagt gattgccaac caaacatata ctattatcgg
     5221 acgggaaacc aagcatcata tggatatcgt aacgaagctg atcaatgaac agttagcaga
     5281 attgaaacaa ttatcaccac aaatggataa tgaacaagca gcaatcttga tggctgtcaa
     5341 tgcgctttcg gatcaattga agaaacaaga aagaatcttg gagctggaag aagaaacagc
     5401 agaattaaag aaaaaaatga tcaaatttac agagcttgaa aatcgtgtga aacgcattga
     5461 agcaatcgag aacgaagctc gggaagtatt gaaagaaaat ggtcaagcag atcataccat
     5521 taaaaatcat gtggaagcac aacagatttt aaacgagaaa cgaaaaggac aaattaaaca
     5581 aaaatcttct aattaagatc cttttatcaa aataagaaaa atttaaaaaa gttagaagag
     5641 aaaggaacgt agcatgctga gtttgctgat tttattttta ttgctaattg cgtttttttc
     5701 tggagcacgt cgcgggtttg ctttgcaaat catattcgcg attggatatg tattgtcatt
     5761 tattgctgcc cagcattttt acaaaccatt agcaagtcat ttggaattat acattccata
     5821 cccagctgtc accccaacat caaagttagc attttttgat caggtatttg cttttcacct
     5881 agatgaagct ttttatgcag gaactgcttt tctgattatt ttgctaattg gctggctgct
     5941 tacccgattt gtgggggttt ttgtccatgg attgacgtat gtgccgatct tgagacaggt
     6001 agactggatt gcaggaggca tcctgtcact aatcatggct tacgttacta tatttttgat
     6061 tttgcagtta cttgcctttg ttccattagt gtggtccaaa atcaattcgg cgagcatagt
     6121 ttcgctcgtt ttatcgttga gagaacgcca tttcttacca acaagatgta tgagctttgg
     6181 gtaacgaata ttttgaatta aggaaaatag tgctggaaaa tatttcgttt tttgtacaga
     6241 gtagtttatt taagaaggct gcgtagcgta tgtccggcac agctcttttt tcatagtgaa
     6301 agataaggtg ataaaatgaa taaccgaatc cttgaaacat tggaatttga aaaagtcaaa
     6361 cagatggtca gacaatttgt agtgactgca caaggaaaag aagaactggc agaattggta
     6421 ccagtgagtg agaaacaaac gatcacaaac tggcttcaag aaactgagga tggattgaaa
     6481 gtgcaacgtc tgcgaggcgg gatccctata cctaaacttg aaaatatccg tccccatatg
     6541 aaacggatcg agatcggagc agacttgaat ggagtggagc ttgcacaagt ttccagagta
     6601 ttgtctacga caagcgaatt gaatcgtttc attgatgatc tttctgacag tgaaatcgaa
     6661 tttgcacgtt tgtatatgtg ggcagatcag ttagtcacga tccctgtttt gagccgccgt
     6721 ttaaaggaag caatcgatga agacggacgc gtgactgatg acgcttctcc tgagttaaag
     6781 agcataagac agaacattcg gcgaagtgaa caagctgttc gtgaacagtt agatgggatc
     6841 gtccgtggga aaaatgcaaa gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc
     6901 tacgttatcc cagtcaaaca agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa
     6961 agtgcttcag gacagacttt atttattgaa ccaaaacaag tggtggattt gaataatcgt
     7021 ttgcgtcaat accaaatcgc tgaacgaaat gagattcaac gtattttaag tgaactttct
     7081 gctgaacttg ttccccatcg tcaagaaatc atccacaatg cttatgtgat tggtaaaatg
     7141 gatctgatga atgcaaaagc acgatttggt aaagaagtaa aagccatcgt gccgggaatc
     7201 agtgaagaca atcatgtcgt cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa
     7261 gtagtttcta atgacatcac aatcggaaaa gactatcaag ccatcgtcat tacaggacct
     7321 aacacaggtg ggaaaacgat cacgttgaag actttaggtc tgttacaact gatggggcaa
     7381 gcaggattac cgattcctgc aggtgaagaa agtcaaatcg gtatttttga ggaagtattt
     7441 gcggatatag gagacgaaca gtcgattgag caaagtctat cgaccttttc ttctcatatg
     7501 acgaacactg tagatatttt atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag
     7561 ctcggtgcag gaacagatcc gcaagaaggt gctgctctag caatcgcaat cttagatgat
     7621 cttggtaaaa agtctgctta tgtgatggca acaacgcatt atcctgaatt gaaagtttat
     7681 ggatacaatc gggcaaatac gattaatgcc agcatggagt tcgatgtgga tacactaagt
     7741 ccaacgtatc gtttgctgat tggcgttcct ggacgaagca atgcgtttga aatctctagc
     7801 cgattaggtc ttgatacaga agttattgat gaagcgaagc agttgatgaa tgatgagagc
     7861 caagatttaa acgaaatgat cacagattta gagaatcgcc ggaaaatggc agagaccgaa
     7921 tatctggaaa tgcgtcattt tgtttctgag gcacaagaac ttcacgatga tttgaaagaa
     7981 gcttacagct atttctttga agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca
     8041 aacgaagtcg tgtcagaggc ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa
     8101 atgcaacagc agattgggca aggaaatgtg aaagagcatc agttgatcga tgcaaagacc
     8161 caattagcaa atctacatca agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg
     8221 aaagaacaaa aaacgttgaa accgggagac gaagtgttgg ttacgactta cggccaaaga
     8281 gggacgcttt tacgtaaaaa cgggaatcag tggcaagtag aaatcggtat attaaaaatg
     8341 aatgtgtccg aagacgagct gacgcctgtt gctcctcaaa aagagccgac acaacgagtt
     8401 atccatgctg tacgatctga atctagtagt cacgtgccga atcaattgga ccttcgagga
     8461 aaacgatacg aagaagcgct aagtgaggta gatcaatatt tggattctgc cattctagct
     8521 ggttatcctc aagtgactat tgtccacgga aaaggaacag gcgcacttcg caagggaatt
     8581 actgattatc ttaaaaacca tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa
     8641 ggcggaaatg gtgcgacaat tgttaaattt aagtaaagct tactaaaggt aagcagatgg
     8701 ttcgctatcc aagtatagaa atgctatact aatcatagat tttaaaaatg gaggtctttg
     8761 atatgtcaca agtaattaca gataagacat ttaatgaaga aacagataaa ggacttgtat
     8821 tgattgattt ttgggcaact tggtgtggtc catgtcgtat gcaagcaccg attttggatc
     8881 agttggaaca agaatatgat gaagaagaat tcagaattgc taaaatggat gtggatgaaa
     8941 atccagaaac tcctcaacaa ttcggtatca tgagcattcc aacattaatg ttgaaaaaag
     9001 atggtcaagt agtagaaaaa gctgttggtg ttcattcaaa agaacaacta cgtcaaatga
     9061 tcgatcaata tttataaaaa cttacagcaa aaataagctg agggtgggac tgaatccagt
     9121 tccgtcctcg gtttttttgc ggttgagtta aggttattca aattaatatt ttttgagtat
     9181 aattacgttg tcaggctgga ttgttttttt gacagacatg ctataataac tttaattgaa
     9241 tatgaagggg agtaccagat gaacgaacga atcaaaaaca aattagcttt acttcctgat
     9301 cagcccggct gttacttgat gaaagataaa aatggaacga tcatttatgt cggcaaagcc
     9361 aaaatattaa aaaatcgtgt ccgctcatat tttactggca gccataatac gaaaacggaa
     9421 cggcttgtta gtgaaatcga agactttgaa tacatcgtta cagaatcgaa tatcgaagca
     9481 ttgctcttag agatcaatct gataaagaaa aatgatccta aatacaacat tatgttaaag
     9541 gatgataaaa cctatccttt ccttaagata acgaatgaaa aatatccccg tttggtgatt
     9601 actcgtaaag tactaaaaga caaagcactg tattttggac catatcccga tgtcggtgcg
     9661 gcgaatgaga caaagaagat cctcgatcga ttatttcctc tgagaaaatg caaacctagt
     9721 cagacgaagg agccttgtct ttactatcat ttgggacaat gtctgtgtcc ttattatttc
     9781 gacgtagatc cgaaagtcta tacgaaaatc gttgatgaag tgaaaaagtt tttaaacgga
     9841 ggacacgaaa aaatcgagtc agagatccat cagaaaatgg aaaaagctgc tgaaaacatg
     9901 gagtttgaaa aagctgcaga ataccgagac cagatccgtg ccattgagac aatcatgact
     9961 cgccaaaaaa tgacaaatac tgatttactg gatcgagatg tatttggtta tgcgattgac
    10021 aaaggatgga tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat tgaacgagat
    10081 gtttcaatgt ttcctttcta taacgaagca gaagaagatt tcttgactta cctcggtcag
    10141 ttttatcagg aaaatgagca tttcattcct aaagaagtct tgattcctga tacgatcgac
    10201 aagcccagtg tagaagcttt gttagctacg aaagtgctgc aaccaaaacg aggagaaaag
    10261 aaaaaattag ttgaattggc gaataagaat gcctcagttg ctttgaatga acggtttgat
    10321 ctgattgcca gaaaacagga acggacgatc ggagcagttg aacgtttagg aaacgcgatg
    10381 aatatcccga ccccaatacg tatcgagtct tttgataact ctaatatcat ggggacgaat
    10441 cctgtggctg cgatggtcgt attcattgat ggaaaaccgg caagaaaaga atatcggaaa
    10501 tataaaataa aaacagtcaa cggaccggat gattatgcat caatgagaga agtcatctat
    10561 cgcagatatt cacgagtgat tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat
    10621 ggaggaaaag gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat
    10681 attcctattg ctggcatggc aaaaaacgat aaacataaaa caaatgagtt gctattcggt
    10741 atagatttgg ctgtagtacc gcttgagcga aattcaccag agtttttctt actccagcga
    10801 atccaagatg aagtccatcg attcgcaatc actttccatc ggcagctacg cagtaaaaac
    10861 agttttgctt cccgattaga tgaaattgaa aggttaggac caaaacgaaa aaaaatgctg
    10921 cttaaagagt tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga attacaagaa
    10981 atcggactgc cgaaaaatgt cgcacaaaac gtatttgata aattacatca ggaatgagga
    11041 aagggcactt tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt
    11101 ttacgttttg ttggtaaata ataaatcgct tacgtctggt gtctaaggat atatgtgatt
    11161 tcttgaagaa ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac
    11221 tacacagctg tcgatcatgt ctctttccaa atccccgatg gaaaaatcat ggggctaatt
    11281 ggccaaaacg gggctgggaa gacaacgact tttcgtttga tcttggattt tttgacacaa
    11341 gaccaaggag aagtgttatg gaatggccat catcttgggg agaaagatta tgatgtcatc
    11401 ggttatcttc cggaggaacg gggactttat ccgaaagtaa cgattcaaga ccaactgctt
    11461 tattttgcag aacttagagg gaaatcaaga aaagagattg aaccaaaaat cgatttttgg
    11521 atggaaaaat ttcaggtaaa agggaaaaag acggataaag taaaatcttt gtctaaagga
    11581 aatcagcaaa aagtccagct gatcgccacg ttaatccatg aacctaaatt gatcattttg
    11641 gatgagccat tcagcgggtt agatccggta aatgctgaat tgctaaaaga tggaatattg
    11701 gaattgaaaa aacatggttc atgtgtcatt ttttctagcc acaatatgga taatgtagaa
    11761 aaaatctgcg atcatttgat catgttgcga aacggaaaaa tggttttgaa tggaaaagta
    11821 catgagattc gtgaatcatt tggccgtacg aagttattct tggaatctgg tttaagccaa
    11881 caggaagtag aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta
    11941 gagattacct tagaagatcc tgaagctggg aaagaaatct ttactcgtgc gactcagttc
    12001 ggctacattc ctatgtttaa ccagcaaccg ccaactttgg aagagatttt caaattgaaa
    12061 gcaggtgaaa tcgatgcgta agttttggat tattaccaaa gatgtctatt taaaaaatgt
    12121 taagtcgatt tctttcttga tcatgatatt agtccctttt atcatgctgg gtatcattta
    12181 tgtagcagga aactttgctc aacaaaatag tgatgtcaat aaaatcggcg tattggctga
    12241 taatgagcaa atcagtcagc aaatgagcca agtcaaaaca gatgattttg agttcaaagc
    12301 agttgattct gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt
    12361 agtagtctca actgagaatg ataagataaa tgggatgctt tatagcgaaa attcattagg
    12421 acaaagtaca caactactga tacaacaaca actgactggc ttgcaatcga tgcttcgagc
    12481 aagtcaatta ggcatttcac cagaagaagt agctagcttg agccaacaag caagtttgac
    12541 tcgacaaaaa gtcaattttg attcaaaagg aaaaatgaca gttggagaag ataactcgac
    12601 agtccaatat gtcgtaagtt atgtagcaac gattgtcttg ttcattatta ttttgacgta
    12661 tgcgcagatc attgcacaag agatcgcttc tgaaaaaggc acgcggatca tggaagtgat
    12721 cctgtccagt acgactgcac aaaagcattt ttacggaaaa ctaacaggtg ttttacttgt
    12781 tgcattaacg caaatggcat tatacggtat tatttttggt ataggataca gtcaattcaa
    12841 agatatggaa atggtcaaaa atatattaga cggtatctca ttggataact tgattggacc
    12901 attcttgtgg tattctcttc tattcatgat ctttggcatt ttgatttttt ctgttttagc
    12961 agctttatgt ggctctttgg tcaacaaagc agaagataca gcaaaagcaa ttttgccagt
    13021 gacttatcta tctctaggcg gatatatgct tggattgata ctaggagctt cagatccaaa
    13081 caatatcgtt attcggatca cttcgtatat tccattctta tcttcatata ttatgccggt
    13141 tcgcttagca aatgagacag tcggtatggg tggagcattg atttccttgt tggtacttgt
    13201 agccgtaaca ttcttattga tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt
    13261 gtatagcgaa ggcggattat ggagttcatt gaaacaatcg atttcaatta tgagaaatga
    13321 acgaaaaaaa gcataaaata tagaaaaata tagaaggaag ctggacattt attgttccca
    13381 gcttcttttt tgtatagacc tatttctatt tttttattat attggatata tatattcatt
    13441 gaaaatagag cataacaact ttataatgtt gagtatacca atttttggag gcgttttaat
    13501 ggaccgctat ttagatttac tttcagagaa gttccctaca acggaagatg tcatcacgga
    13561 aatcatcaat ctcgaagcaa ttttgcagct tccaaaagga acagagttat ttttgagcga
    13621 tatacatggc gaatttcctg cttttgatca tattctgcgt atcggttcag gaaatcttaa
    13681 agaaaagatc aaagaccttt tctctgaaca aatgacggaa acggagatca gccaattcac
    13741 tatttttacg gcttatccag aatatgtgtt gacgactgat tggtacaaaa aacaagataa
    13801 atcccagctg atccatcgtt tgatcgatct tttacggttt acgacagtca aatacactcg
    13861 atcaaaagtt cgaaaaggat tgccgaaaga atatagctat atcattgagg aactcttgta
    13921 tatcgatgat cgtatcaacg ggaaaaaaga ctatgtaaaa aaaatcattg aacaacttgt
    13981 cttgatgaag gaagaagaaa aatttttaac aaaacttgcg cagacgatcc aaaaatctgt
    14041 catcgatcat ttgcatatcg tgggtgatat ttttgatcga ggaactcaag cagccaaagt
    14101 aatggatcga atcatggatt tttcctctgt agatatccag tgggggaatc atgatatttt
    14161 gtggattggt gcgtattgcg gctctgaggc atgtttattg acgcttttac ggattgccgc
    14221 acgctacaac tatttatttg aattagagaa agaatatgga ttaaatttac gtccattatt
    14281 ttcttttgcc catcaaacct atcagaagaa tccagtattt acccctaaaa acaggaaaga
    14341 tctatctgaa agagagcaag aagaattaga aaagatccat caagcacttt ctatcctcca
    14401 attcaaatca gaacatcagt tgttgaatcg acgacctgaa tttaaaatgg acgatcgtaa
    14461 attgttggaa aaaatcaact atgaggaatc aacgatcgtt ttagaaggtc agctctatga
    14521 gttgaaaggg acttgttttc aaacgatcca gccagatgat cctaaaaaat tcttggaaga
    14581 ggaacaaaaa gtgatcgata gtttgatgta ttcattccag cactcgttaa gaatgcaaaa
    14641 acacatgacg tttttgatcg aaaaaggggg aatgtatctc acgaacaatc agcatgtact
    14701 gttccatggc tgtatacctg tagatgaaaa ggggcagctc ttagcatttg aattagacca
    14761 atcttatcgc ggaaaagaac tgctgggatt ttttgaaaaa cagataactg aaagcgcaaa
    14821 agatttgact gcaaatgaag atttggcgac cgatttgatt tggtatgcgt ggagcgggcc
    14881 attttcgcct ttattcggca agagtaaaat ggcaactttt gagcgctatt ttctcacaga
    14941 gcctcatacg cacgtcgaaa aaagcaatcc ctattatcac ttgcgagatg aagagtggtt
    15001 cagtgaaaaa attttggcag aattcgatgc aaaagaagaa ggttcagcaa tcatcaatgg
    15061 acacacgcca gtaaaagtaa aaaaaggaga gtcaccgatc aaagcaaacg gaaaagcatt
    15121 tgttattgac ggaggtctat ccaaagctta tcaaaaaaca accgggattg ctggctactc
    15181 attactatgc aattcttacg gtttccaaat cgtgacacat tatccgttct tgcctattga
    15241 acaacaattt gcaaaaaaat ctgatcaaac gaatgtaaaa aaagtgatcg agcagcagct
    15301 cccacgcaaa cttaatcgga atacgacaaa aggaatggaa ttacagcaac agattactca
    15361 actccaaagg ctgttggatt atcgaaagga cgattgagaa aaatgctagc caacattgct
    15421 caatagacta aaaagcgact gcaacaaaag gcgtgtcgca gtctccttcg gaattagctc
    15481 aaaaaacggt aaaatattag aagctatttt tctgtttatt tctctaatac tcaggtcagg
    15541 gcgctgattt cacgacctct tccttaaacg gtgttctcct gccaactcct cggtcacaag
    15601 tcacaatatc caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt
    15661 tgaacggcag gctcacgacc taatcagaaa aacgattaaa attcattcac aaagaaatcg
    15721 aataaacgct gttcagatga atctacacga tgagaaagtt ccggatgcca ttggacacca
    15781 aggattttgt gcatattatc tttactttgg actgcttcaa tgagtccatc gttggaaaag
    15841 gcgatggctt ttaacaaagg agaaagatct ttgactgcct gatgatgata ggaattcaca
    15901 aagtatgaat cagggagaag gtcacttaat aaactgtccg aaacgatttt gacttcgtga
    15961 gttgcaaact gcggggcagt gggctgttgt tcgtgtttta cggaccattt tggatataat
    16021 gaaagatctt gataaagtgt tccctccaac acgacattca atagttgaag tcctcggcag
    16081 actgcaaaaa tcggttttct ttgtttcaat gcttccaaga tcaatgcctg ttcgaattgg
    16141 tctcgttgaa tatttgtttc cgtcagtttt ggatgagggt cttccatgta aagttgcgga
    16201 ctaacatcct gaccgcctgc aagaatgagt ttgtcgattt tctcaattag ttgaggcgct
    16261 gaatctggag acatgactgg cagaataaga ggtaatcctt gagcatctaa aactgcatcc
    16321 acaaatccct gcggtgtata gcttacttga tttccttgga aagtatctgt cgctcgaatc
    16381 agttgattcc cggcaatgcc gatgattggt ttcattttta ttccccctaa agtagactaa
    16441 ttgtaacaag gatttaaaaa aatttaaact atgtaagact tgcccctata cttatctgtt
    16501 cttgctgtgt tactatctgt aaagagaatt ttcgtgtgaa attcatgacg atagttgaaa
    16561 atattgctta atttgttata ataaaaagta agcagaaata aaaaggttgg gcgtaagctt
    16621 caaggatcta gttcttcaag gggtgagaag gacattttat tgaaggggcc tgcctatact
    16681 aagttatctt tagaggtaca tactttcaat gaaaaaagaa agcatgtacc tcttttttaa
    16741 atcgaaagga agtgaagcaa gtgttagaag ctttggaaca aatccgaaaa gcagaagaag
    16801 aaaatgagaa gcgtgctcga accctacggc agcagttgaa gacctatgca gaagaaaaag
    16861 agcaagaatt ggagataatc caaacgaaca gtcggcaaca gctgaaacag ttggtagaag
    16921 aaaaagaaca attcgaaaag caagaattag aaaaagaaaa aatgaaattg aaagcagaag
    16981 cagacaaggt gcaagcaact ttgaaagacc aatatcaaaa gcatcagagt caagcaatcg
    17041 gtgcaatcat tgaaagggtg aaagaaactt atggccgtca ctaagatgga aaaagtgacc
    17101 ctcatttcag ataaaagaaa tcaggagagg attttacaag cagtacaagg aatccaaaat
    17161 gttgaaatcc gggatctgta tcaaaataga acaaacaacc agtgggtaga aaaatatttt
    17221 ccagatacca cgattattga taaagaagca aaattaagcg ctttaggcaa tcggctgaaa
    17281 gacatccaag aagctatcca attcatacaa caccacggcg atagccgtca aaagaaactc
    17341 catttgaaac gtcaagaact ttccttacaa gacttggaag caaattattc cgaagaaaat
    17401 ttcatgaata aattagaaga agtccttgat ttaaaagaaa agtgggaaca gctaactgaa
    17461 aaacgtgatc gtttagaaga agaggaagaa tggcttcttc attggcaaca gctggatatt
    17521 gctccaagta tgtatgcaag taagacgacc atttttgaga tggggactgt gagtgcagca
    17581 aattttgaaa gtttcaagga agaactaagt aagcttgatg acgtttatat ggaagaaata
    17641 gattatacgc caaaacaagt ttatctttcg tatgttgctt taaaaaaatc agttgctgct
    17701 atagaagaaa ttgctgggaa atacgggttc aataaagaaa cgtatcctta tgaacaaaat
    17761 cctaaagaaa aattgcaaga aaccaaacag caattatcca ttgttcacga gcagcaaaaa
    17821 aagataacga cagcgttagg tcagtgcagc ggctatatcc aagaatttga atgggtagaa
    17881 gaagtcacac ttgccgttgc agaacgggaa aaagtaaaag aacggtttat ccatgccact
    17941 tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa aacagaattt gatgcatgtg
    18001 ttaaacgaaa ctgtatcaga agaagatata tacgttagtt tcgaaacacc aaactcagaa
    18061 gagattcagc aggaagtccc tactaaattg aaaaaccatc cgcttatcga accttttgaa
    18121 ttgctgactg aaatgtacag tctgccaaaa tatgaagaag tagatccaac gccttggatg
    18181 acaccatttt atcttgtctt cttcgggatg atggtcgcag atgttggtta cggattgctg
    18241 atgctcattg ggacaatact agctcaaaaa ctatttgttt taccccgcgg gatgaaacgt
    18301 ttcgtaaaat tctttgagat tctctcggta ccatcgatca tctggggatt gatctacagt
    18361 tcatttttcg gacaggcatt accaaaagaa ctatttggta tccgcttgcc tttcccaatc
    18421 ctatctacga cggatgacgt caatacgatt ttgattttat ccgtgatttt cggtctgata
    18481 cagatcttgg ttggattatt cgtggcagca aaggaaaata tcaggaggaa agattacttg
    18541 agtgctgtca gtgatggttt tgcctggcaa gggatcctga ttgggatcgt tattgcattg
    18601 attggtgtca tgttgataaa aaatacagca tttgtttatc ttggcgcggg gctatcgatc
    18661 attgctgctt tatgtatctt aatcgtgccg attatccaat cgccgtctaa agtcaagggt
    18721 gctgccaaag gagcgtataa tctttacgga ttgactggtt atatcggtga tttagtcagt
    18781 tatacacgtt tgatggcact aggtatttca ggcggaagta ttggcgcggc attcaatatg
    18841 ctagttgctt tcatgccgcc agccgctcga ttcagtgtag ggatcctttt gatcatcgct
    18901 ttacacgcac tgaatatgtt tttgacactg ttaagtgcct atgtccatgg tgcacgattg
    18961 cagtatgtcg aattttttgg aaaattttat acaggaggcg gacgtgcctt tgacccgctg
    19021 aaaacggcgg aaaaatacgt caacatcaat cataagaaaa aaaacaataa ataattggag
    19081 gatttttata caatgatgga ttacttaatt agtcaaaatg gtggaatggt atttgcagta
    19141 ttagcgatgg caacagcgac gattttttca gggattggtt cagctaaagg tgttggaatg
    19201 acaggtgagg cagcagcagc gttaacaaca agtcaacccg aaaaattcgg acaggctttg
    19261 attctgcaat tgcttccagg tacgcaaggg ttgtatggat tcgttatcgc ctttttgatt
    19321 tttatcaact taggaaatga tatgtcagtt gtacaaggat tgaactttct aggcgcttct
    19381 ctgccgattg cttttacagg tttgttttca ggtatcgccc aagggaaagt tgcagcagca
    19441 ggtatccaaa ttttagcgaa aaaacctgaa catgctacaa aaggaatcat ttttgctgcg
    19501 atggttgaaa catatgcgat tttaggtttc gttatttcct tcctattagt attgaatgcc
    19561 taagcataaa ggaggaaaag cagatgaacg ccattgaaaa aatcatctct cagatgaatg
    19621 aagcagcaga gcaagaaaga gcggcgttag agcaagaaga gcgaatgaag atcgatcaaa
    19681 actttgaaca aaaacgtacg caaatcgaaa cagaacatca aaaacaaaaa gaaaaacaaa
    19741 tcgaattact tgaaaaaaaa taccgtcagc ttaggaatcg ccaacaagta gaagtacgac
    19801 aagaaaattt gaatgcgaag caggaatttt tacgacgatt gtttgcagat gcaatagctg
    19861 aaatggagaa ttgggatgaa tctgaacaaa tccagtttat caaaaattca ttgtattcat
    19921 tacctttgac aggcaaagta acattcattg ctggagaaaa atcagcagct tatctttctc
    19981 agacattgct ggatgaatgg aacaatgaac ttccctttat gatggtttta agtgatgaaa
    20041 ctgttgctga tcaagcgggt tttctgataa acgatcaagg agttcagtat aattttcttt
    20101 tcagcagcct tgtacaagat atccaaggaa cgatgagttt tgagattgcc aatcaattat
    20161 tcgaataagg agggatcagt gtatggaata ccacgaacta aatcccttga ttcgcggaag
    20221 agagcttgaa cttttgtcca atgagacatt tgaacggatg attcaaacaa attctgtcga
    20281 agcacttggt gaaatattga aatccacgat ttacagtcca tatatttatg acggatatga
    20341 atatgacttc gaacagaatc ttttaaagga gcagagccgt ctatttcgct ggttaaagga
    20401 atcagcaccg gagcctgaaa tcgtttggat ctatacgatg cgctatactt tccataatct
    20461 gaaggttttg acaaaagccg agatgacaaa tcaaaatctg gaccatttgt atatcgacga
    20521 tggattttat tcattagaaa ccttgaagga tgccattcat acgcaagttt caaatgaact
    20581 ccctgaaagc attatggaca gtatacgaga agtacgtgat tattttgaag agtctaccat
    20641 tcttcaggga attgacgtga tttatgatcg acagtttttg acagagcagc gacggcttgc
    20701 cgataagcta ggatatcaag aattgctgac agaagtcatt gcttttattg acttgaccaa
    20761 tatcacaacg atggctcgag gaatattgca gaaaagaaca tatgggttca tgacgacggt
    20821 actttccagc tcgggaagta tcgaaaaaga agtatttttg aactttgtag ataaagatat
    20881 ggaagattac acgaattttc tattagagac agattatgct gattttctgc gccctgtcat
    20941 ccgtgaggga aatcttgatc tcgtaaagct tgaacagttg aaagatgatt atctaagttc
    21001 attgtatcaa gaagcacaaa cccaggcttt cggaccactg ccactactag cctttttgaa
    21061 cgcaaaagag gtagaaagca aaaaccttcg tttactggtt atcggcaaaa gaagccattt
    21121 cgaaccagaa acaatcagaa agcgggtgag acaagtctat gacgcataag atcggtgtag
    21181 ttggcgacaa agattctgtt ttgccattca aattgtttgg ctttgatgtt cgttatggta
    21241 caaccaagca aaccgttcgt caggcaattg aagaaatggc aaagaatgag tatggcgtga
    21301 tttatgtcac agagcaatgt gcagcattga gcccagaaac gatcgaacgt tacaaggaac
    21361 aaatgatacc tgctatcgta ttgattccca atcatcaagg atcattaggg atagggctgg
    21421 aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa tatcttatag tatgaaaagg
    21481 agatagaaat tttgcaaatt ggaaaaatca taaaagtctc cggacctctt gtaatggctg
    21541 agaacatgtc tgatgccagt atccaagaca tgtgtctagt gggagatcta ggagttatcg
    21601 gagaaattat cgagatgcgt ggcgatgtgg cgtctattca agtatatgaa gagacttctg
    21661 gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc tttatctgtt gaattaggac
    21721 cagggatcat ttcacaaatg tttgacggaa tccaacggcc attagatacc tttatggaaa
    21781 tcacacaaag taatttctta gggcgcggag ttcaattgcc cgcattggac catgagaaaa
    21841 aatggtggtt cgaaccaaca gtagaagccg gagaaacggt gagtgctgga gatgttatcg
    21901 ggatcgtaga agaaacaaaa gttataaaac ataaaatcat ggtgccaaat gggataaaag
    21961 gaacgatcaa aacaatcgaa aaaggttcat tcactattga tgaaacagta tgtgtcattg
    22021 aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa atggccagta agacgtagtc
    22081 ggccaatcaa agaaaaatta aacccggatg cacccatgat cactggacag cgagtgattg
    22141 atacattctt cccagtgaca aaaggaggag cagcagctgt acctggtcct tttggtgcag
    22201 ggaaaacagt cgttcagcat caaattgcaa aatgggccga tgtggacatg gttgtctatg
    22261 tgggatgtgg cgagcgtgga aatgagatga cggatgtttt aaatgaattc cctgaattga
    22321 ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt attgattgct aatacatcaa
    22381 acatgccagt agcagctcga gaagcttcta tctatacggg aatcacaatt gcggaatatt
    22441 tccgtgacat gggttatgac gttgctatca tggcagactc cacttcacga tgggcagaag
    22501 cacttcgtga gatgagcgga cgtttggaag aaatgcctgg tgatgaaggt tatccagctt
    22561 atcttggttc acggcttgcg gaatactatg aacgatctgg aagagtcatt gcattaggat
    22621 cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt atccccgtct ggaggggata
    22681 tttcggaacc agtcacacaa aatacattgc gggtcgtaaa agtattctgg ggtcttgatt
    22741 ccagtttagc ccaaaaaaga cattttcctt cgatcgattg gatacaaagc tattctcttt
    22801 attcaacaga agtagggaag tatatggatc atatccttca agctgactgg gcatccatgg
    22861 tcaccgaggg catgcgtatt ttgcaggaag aacagcagct gaatgaaatc gtgcgtttgg
    22921 tagggattga ttccctttca gataatgacc gtctgacatt agaagtggca aaatccattc
    22981 gtgaagatta tttgcagcaa aatgcttttg atgatgtcga tacattcact tcaagagaga
    23041 aacaattcaa aatgttgaag atgatcttga cctttggtga agaaggacgc cgtgcgttgt
    23101 cgttaggtgc ttatttaagt gagatcatgg acggtaccgt atcgattcgt gaaaggatcg
    23161 gacgaagcaa atatatcccg gaaacagaat tagaaaaact agatcaaatt attggtgaaa
    23221 tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat gagcaatgat taaagaatat
    23281 cgtacgatcg gtgaagtcgt cggtccattg atggcggttg aaaaagttgc tggtgtcaaa
    23341 tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga ttcgtcgtgg acaagtattg
    23401 gaagtccaag aagataaagc aatggtccaa atattcgaag gaacaagtgg tatcaattta
    23461 cgtgactctt ctgtacgatt tttaggccat cctttagaac taggtgtatc ggaagacatg
    23521 atcggtcgag tattcgatgg tttgggacga ccgaaagata acggaccaga gatcctgcct
    23581 gaaaagcatt tggatattaa cggagaagta atcaatccag tagctcggga ttatccagat
    23641 gagtttatcc aaacaggtat ctctgcaatc gatcatttga atacattagt tcgtgggcaa
    23701 aaattaccag tattttctgg ctctggtctt cctcataaag aattagctgc tcagatcgcg
    23761 cgacaagcaa cggtactcga ttcatcagat gatttcgccg tagtctttgc tgctattgga
    23821 atcacttttg aagaagctga atttttcatg gaagattttc gtcaaacggg cgcaatcgac
    23881 cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa tcgaacgtat cgctactcct
    23941 cgtatggctt tgactgctgc agaatatctt gcttatgaaa agggaatgca tgttttggtt
    24001 atcatgacag acatgaccaa ctatgcagag gcattgcgag agatttctgc agcacggcga
    24061 gaagttccag gaagaagagg atatcctggg tatctttaca cgaacttagc tactttattc
    24121 gaacgagcag gaagaatccg cggactaaaa ggatcggtta ctcaaattcc gattctaaca
    24181 atgccagaag acgataaaac tcatccaatc cctgacttga ctggttatat tacagaagga
    24241 cagatcatct tgactagaga gctttacaag agtgggattc agccaccaat tgatgtactc
    24301 ccgtctcttt cgcggttaaa agataaggga actggacccg gaaagacaag agaagaccat
    24361 gcagctacga tgaatcagct ttttgcagcc tatgcacaag gaaaacaggc aaaagaatta
    24421 gccgtagttt taggtgaatc tgctttatct gatgtagaca agatctatgc gaagtttgct
    24481 gagcggtttg aaaaagaata tgtcaatcaa ggattctaca cgaaccgttc gattactgaa
    24541 acacttgatc tagggtggga actgctatcg atgttgccaa gaaccgaatt aaaacgaatc
    24601 aaagacgaca tgctggacaa atatctgcca gaagggaagt gagttctgat ggcacgattg
    24661 aatgtgaatc cgacccgcat ggaattgact cgattgaaaa aacagttgac aactgccaca
    24721 agagggcata aattattaaa agataagcaa gatgaactga tgcgtcaatt catcctgttg
    24781 attcgaaaga acaaccagct gcgtcaagaa atggaagagt ctatgacaga agcaatgtct
    24841 gattttgtat tggcaaatgc ttcgatcacg gaacctttca tcgaagaatt atttgttttg
    24901 ccggcggaaa acgttgattt gtctatcgta gacaaaaata tcatgagtgt aaaagtacct
    24961 gtgatgaatt ttgaatatga tgaacagctg acagacacac ctttggaata cgggtatttg
    25021 aattccagtg ccgaactgga tcggtcaatc gatcggttta ctaccctgct tccaaaactt
    25081 ttggaactga cagaaatcga aaaaacttgt caactgatgg cgagtgaaat cgaaaagaca
    25141 cgtcggcgag tcaatgcatt agaatatatg acgatccctc aattagaaga aacgatctat
    25201 tatattcgta tgaagctgga agaaaacgag cgcgcagaag taacaaggtt gatcaaagta
    25261 aaaaatatgg gtaccactga ctaagtggta tcttttttta aaagaggaaa acgatgatta
    25321 aacaaagcaa acaaataaag aaacgtttgc cggctgttca gcttctcgca ttaggatttt
    25381 ttctcctgat tttagttggc ggcggcctac ttaccctacc attttttagt aatagtgggc
    25441 atgggacaaa ttttatcgat gcgcttttca ctgcaacttc tgctgtctgt gtaacgggat
    25501 tgacaacatt gaatacagca gagcattgga acagtgctgg tcaatttttg atcatgcttt
    25561 tgattgagat cggcggtctt ggttttatga tggttccgat tctatgcttt gcattgataa
    25621 agaagaaagt cagtctcagc acacgtatta tattaaaaga agcgctgaat ctagaagaag
    25681 tatcaggtgt gatgaagctc atgctttaca tcctaaaatt ggcctttgtt atccaattga
    25741 ttggtacggt tgctttatct tttgtatttg tgcctgaatt tggctggttg aaaggtatct
    25801 ggtacagttt gttccatgca atctctagtt tttgtaatgc agggtttgat ttgttaggag
    25861 acagtttagc caatcaccag caaaatgttt atttgatcat ggtggtatct gctttgatca
    25921 tttctggtgg gttaggcttc atcgtatggc gtgatttact ggaatatcat cagaaaaaga
    25981 agatgacgat tcattcaaaa atcgcattaa ccgttacagg taccctttta ttaggcggat
    26041 tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct agtggaaggg aatatgtttc
    26101 aacgattggc aaacaccttt tttatgagtg tgacacctag aacggctgga tattattcag
    26161 ttgattacct ccatatgagc catgcaggtc tgattttaac gatgtttttg atgtacatcg
    26221 gcggaacatc tgggtcgacg gccggcggat tgaaaacaac tacgttaggt gtgttgctga
    26281 tccagatgcg ttcgatgttg aaaggacgta cgagagcgga agcatttggc cgtaccatcc
    26341 gacaaggcgc agtgttgcgg gcacttactt tattttttgt tacgttgacg ttgtgtgtag
    26401 tcgccatcat gattttatcg gtgactgaaa cgatacctaa aacttctggc atcgaatata
    26461 ttgcttttga ggtcttctct gcgtttggta cagtaggtct tacgatggga ttgacgccag
    26521 acttgactgc aatagggaaa ttgatcatta tagcgcttat gtacattgga cgagttggtg
    26581 tattgactgt ggtcttctct ctgatgacaa aagcacaaag aacacaatca aaatacaaat
    26641 atccagaaga aagtgtcatg attggataat ttcatcttct gacagaaacc tagtgattta
    26701 gtaaaataag aaaataaaat aatttttctt gcttcttgga tactttaagt gcaaaatgtt
    26761 cgtttatttt ttgaggcact acccgttgtt gaacctcggg tagtgcctct tttttattga
    26821 aaggaatcaa taagatttga ttcctttatt tatcaatagg ttataattca tattttttta
    26881 ttttttcttt tacgatactt agtccagaag ctaaaatttc aaggttatta gaggataggg
    26941 aaattcgaac gaatgcatct ggttgcgcac gtgtagtaaa gcgtctggaa tgataaagtc
    27001 ggataccgtt agcaagaaag tcattctcaa ttatcttagg gtcaatctca ggatcaactg
    27061 gaagaattcg atagtaaggg acacgattcg ttggtttttc tactttgaaa agagaatcaa
    27121 aaagctgatt ggctcttttt gtaagactta atttagtttc taagatccta tttgcttcat
    27181 ttgaatggag gatttgagtg atgacttcca catctaatcc agaagttttc acatttatat
    27241 taaaaattgc ttgttctatc ttgctttgga catgtactgg aactacaaga taagcaactc
    27301 gcaatccaga acaaataaat tttgtcattc cagctaagta gattgtttgg ttaggtaata
    27361 aattttggaa aggaggaatg atcgtatgtt gatagtaagt tgttaaaaat gaatggatat
    27421 catcttcaat aacccataga ccttcttttt caatcacttc ggctaattgc tctcgtcttt
    27481 tgtgagagat ttgaaaccct ataggattat tacaagaagg cataagaaag acaccatgaa
    27541 tcttcttttt tctacattcc tgaatcaata tatcaggtcg cattccttgc tcgtcaaaat
    27601 caattggaac gatctctaaa tgatataact gagccaattc aatgaaatta gcgtatgtat
    27661 atcggtctgt tgctattcta tcacctggag agaaaagtgc agaaagtgtg atagccaatc
    27721 cattttgaac accggaaaca atagatagag attctaagga cggaatgact ccttgagttt
    27781 ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc tttgggatct tggtaattaa
    27841 gcaaaccaac aagatttggg ttattactag ttgattgaat aaacgggaca acgaatttat
    27901 tacattcttc aaaagaacta accagaccaa aatcaatgat atgttcggtt acttcattgg
    27961 tagagatggt gatggactca aagacattgg gagaaataaa tgtgccactt ccaatattcg
    28021 tatacagcaa acctttctca attccatatt tataagcctt gccaacggta gtaaaattta
    28081 aatctaaata atcagctagc tctctttggg agggtaactg agtgtttttc tgtaatttat
    28141 tctttaaaat atcttgttct aattgttgaa tcaacgatcg ataataagga cgcttcaatt
    28201 tttgtttgtc tggtcgccaa gttaacgggt aattgtcata agaattaata ggcattttct
    28261 ccactcctta aaattgtata ccatacaatt ttaccattga ttgtattgat tttagaagtt
    28321 aatcttttgt gaggagggaa ttgtctatga aaattattaa ttttgaggaa gtagtagctt
    28381 cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg tttttttgat tatgaaagtg
    28441 gtaaaatcag ccaaaatcct cgattggtgg aaattttacc tgacggaaaa ggcaaaaata
    28501 tgtttgcgat gatgcctgct tatttggggg agaacagata ttttggcaca aaagtcatta
    28561 ccgcttttcc ggataatcat cagtatcatt taccttctca tttaggagag attttcttgt
    28621 ttgatgctca aaacggacta cccaaagcat tgataaacgc aaatgcagtc acctggattc
    28681 gaacagctgc tgtatctgcg ctggcaacag attacttagc aaatccgcat gcagaaacac
    28741 ttgctttaat tggagctggt caacaagcat tgtcgcattt gactgcgatt tctaccattc
    28801 gtcccatcac tacagtgttt gtttacgata ttttagaaga aagacgaaat acttttatcg
    28861 ctaaagctaa aaaacagtat cctaaaataa cgtttataaa tgcaatatca gtagaagatg
    28921 cagtacgtcc agcggaaatc atttgtacgt tgacctctag taaagaggca tttcttcaaa
    28981 aagagtgggt aagggaagac gcacatatca atgctgtggg cacatttact cctgatacaa
    29041 gagagataag tacatcgttg atggaaactt ctcaaatcta tgtagatgat tactcagctg
    29101 ccttaacaga aagcggggac ctgcttatag ccatggcaga aggagattta gaggaatctt
    29161 gtattgttgg tacattaggc gaattagttt ctgagaaaaa aacattttat aaaggagaat
    29221 cgaggccgac gattttcgac gcagttggtc tagctattga agatctttgt tgtgcagaat
    29281 atatttataa caaattagag aaaggggctt taaaatgaac ttagattatt acgtagttga
    29341 tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg gtttatgtgc tagaagaatg
    29401 gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac aatttatctg aaacagcatt
    29461 tacagtgaag aaaaaccaag aatttgagct tagatggttt acgccagatc gagaaatcga
    29521 tctttgtggt cacgcgacat tagcaacagc atttgtttta tttaactatt acaaaatacc
    29581 tgatgaaacg attaaatttt ctactcaaag cggtaatcta tttgtgacaa gaattaaaga
    29641 ttattattac atggattttc ctagtattat gccgaaaaaa gtgccgatat tgtctgaata
    29701 tgaagaagcg attggggcta agattaaaga agcctactta gcacgtgatt tatttttcgt
    29761 attagaagat gaaaaaacgg tggctaaatt gcagccagat tttacagcta tcaaaaattt
    29821 tgaattagga ataggagtca tcgtgacagc agaaagtgtg caaaaagact ttgtttctag
    29881 aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag cacattctaa
    29941 tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc
    30001 atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa
    30061 agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg aattaaaaat
    30121 aaattggtgg agagtggcaa ccttatagct acaatggttg ttaaaaaaag acgcaaactt
    30181 tttcaattta atgaaaagtt tgcgtctcat tttaattatt ggtgtttgtt ttttattcaa
    30241 tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta taaccatcag
    30301 ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa tccttttaag tcagtaatct
    30361 caagaccggc agctttatgt tgctcgtgta atttttctag atcatcagct gctatagcga
    30421 tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa gtcaactcta
    30481 attcatagtc atccccagga agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat
    30541 cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt gatgcttcaa
    30601 gatctttcac tcgaacacat gtgtgagcca ttttcataga ttcatctctc cttttccttt
    30661 tactcaatca taacaaaaag aagtaaggaa atagttgatt tgtgcttgtt catgagaaat
    30721 ttctcatgta aaatggatag aaaataaaac tgttaaggtg gaaactattc atggataaac
    30781 caatttttgg tgaaaaaaag caatttgctg attatcaaac ccgttatgca gcatatattg
    30841 tcatagaaag agatcagcag attgcagtaa tcgaggctcc aaatggtgct ttttttcttc
    30901 ctggcgggga aattgaagga gaagagacga aagaagaagc gattgtccgt gagctgatag
    30961 aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat gagtattttt
    31021 actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt gctgattctt
    31081 ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg agccctcaag
    31141 aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga tggatcgaaa
    31201 aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt tatttattac
    31261 agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca
    31321 tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag accagcatat
    31381 tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc ttgatcaata
    31441 ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt atttagtact
    31501 tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa
    31561 cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt gacgatgttc
    31621 atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac taaacttgct
    31681 tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc
    31741 gaatggagat gactgccgac acggcgtgcc atcacttcga tctctaggaa atcttcatgt
    31801 gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag tgccccgtta
    31861 taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg ttcctggaca
    31921 cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac agcagcggca
    31981 atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt accatcgatg
    32041 tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt tatttcaatc
    32101 aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa taaatcataa
    32161 tgatcacgca aggacggatc aaccatttct tttggaaaga aaaatcgctc atctccttgt
    32221 tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat caaagttccg
    32281 tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca
    32341 taaggtagat cgtagctaga attcacagca gtgtaataaa caggattata tccgaccttt
    32401 tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga aaaagtagca
    32461 gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat aggatcagca
    32521 gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt gaggtattct
    32581 tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag ttcaaaactg
    32641 ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa aattacttcc
    32701 ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg gctaacgata
    32761 taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc ataaggacga
    32821 atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt gaaataagca
    32881 tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat ttggacaact
    32941 tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa accttcttct
    33001 actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc tacagtgatg
    33061 gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc atgaccgaca
    33121 tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca gcctccgttg
    33181 cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct tgaaatctca
    33241 taaacgccta atgaatgagt aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa
    33301 gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt tcgaattgat caaatctaag
    33361 attacttgat gctggacatg gatatagtta tggactggat cacgaaaaac tttttccata
    33421 ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt tattacgttg
    33481 ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt ttagccagtc
    33541 ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg caaatgcttc
    33601 tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg ccattgtagc
    33661 aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag attgataaaa
    33721 tcgcttcatg acttgcccgc gtttttcctg attaccgttg acacttaagt acatcataac
    33781 agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc catggatgct
    33841 taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta tcggtgcctt
    33901 tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta tggttatttt
    33961 ttttgtgtca ctttggggga aaatcaaaga aatattcaaa attccttcat ctgatacgat
    34021 ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatctttca gtgtttggat
    34081 cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg tgcagtcata
    34141 ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta atatatctgt
    34201 taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg
    34261 gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat actctaaaat
    34321 caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt tgacaaaaca
    34381 aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat ggaattatca
    34441 caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa
    34501 gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct gtacatacgt
    34561 tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa attagttcca
    34621 gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat
    34681 aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat tgctactttt
    34741 acgaaagaac tgcatgttag tttgaaagac cgtcccacag gagggaaggt tcgaatcgat
    34801 tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt ggattttaca
    34861 gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat aaggagtaga
    34921 ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa
    34981 ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt catggatttt
    35041 tctgatttag ctaaccagat ccaaaactat cttgggaaat cagacagtga aattcgtgat
    35101 tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc attaggggat
    35161 aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga agtaaaccat
    35221 ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa agtcaacgcc
    35281 tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga agatacagat
    35341 ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga tgaagaaatc
    35401 gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga tgatgaagaa
    35461 gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga tgatgatgaa
    35521 gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt ttcttacgag
    35581 aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacga ttt
//
LOCUS       DARBFD010000016        35400 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000016 DARBFD010000000
VERSION     DARBFD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.016"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..649
                     /locus_tag="SCK08_000812"
     CDS             8..649
                     /locus_tag="SCK08_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200723.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="SCK08_000813"
     CDS             708..1148
                     /locus_tag="SCK08_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200724.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="SCK08_000814"
     CDS             1160..1828
                     /locus_tag="SCK08_000814"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1200725.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="SCK08_000815"
     CDS             1892..2515
                     /locus_tag="SCK08_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEG1200726.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="SCK08_000816"
     CDS             2571..2957
                     /locus_tag="SCK08_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEG1200727.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="SCK08_000817"
     CDS             3170..4561
                     /locus_tag="SCK08_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEG1200728.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="SCK08_000818"
     CDS             5172..5540
                     /locus_tag="SCK08_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEG1200729.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="SCK08_000819"
     CDS             5720..6661
                     /locus_tag="SCK08_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEG1200730.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="SCK08_000820"
     CDS             6730..7461
                     /locus_tag="SCK08_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEG1200731.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="SCK08_000821"
     CDS             7605..9785
                     /locus_tag="SCK08_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEG1200732.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="SCK08_000822"
     CDS             9805..10287
                     /locus_tag="SCK08_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEG1200733.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="SCK08_000823"
     CDS             10303..11064
                     /locus_tag="SCK08_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1200734.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /locus_tag="SCK08_000824"
     CDS             11195..12529
                     /locus_tag="SCK08_000824"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEG1200735.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="SCK08_000825"
     CDS             12836..13321
                     /locus_tag="SCK08_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEG1200736.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="SCK08_000826"
     CDS             13314..13997
                     /locus_tag="SCK08_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEG1200737.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="SCK08_000827"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="SCK08_000827"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEG1200738.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="SCK08_000828"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="SCK08_000828"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEG1200739.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /locus_tag="SCK08_000829"
     CDS             17613..18242
                     /locus_tag="SCK08_000829"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEG1200740.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="SCK08_000830"
     CDS             18294..18626
                     /locus_tag="SCK08_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1200741.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="SCK08_000831"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="SCK08_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEG1200742.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="SCK08_000832"
     CDS             19452..20825
                     /locus_tag="SCK08_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1200743.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="SCK08_000833"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="SCK08_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEG1200744.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="SCK08_000834"
     CDS             21880..22320
                     /locus_tag="SCK08_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1200745.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22464..23165
                     /locus_tag="SCK08_000835"
     CDS             22464..23165
                     /locus_tag="SCK08_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEG1200746.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23162..24283
                     /locus_tag="SCK08_000836"
     CDS             23162..24283
                     /locus_tag="SCK08_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEG1200747.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24298..25530
                     /gene="pepT"
                     /locus_tag="SCK08_000837"
     CDS             24298..25530
                     /gene="pepT"
                     /locus_tag="SCK08_000837"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEG1200748.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25662..26570
                     /locus_tag="SCK08_000838"
     CDS             25662..26570
                     /locus_tag="SCK08_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEG1200749.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26605..26877
                     /locus_tag="SCK08_000839"
     CDS             26605..26877
                     /locus_tag="SCK08_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEG1200750.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26888..27934
                     /locus_tag="SCK08_000840"
     CDS             26888..27934
                     /locus_tag="SCK08_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEG1200751.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28185..30794
                     /gene="clpB"
                     /locus_tag="SCK08_000841"
     CDS             28185..30794
                     /gene="clpB"
                     /locus_tag="SCK08_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEG1200752.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30945..31631)
                     /locus_tag="SCK08_000842"
     CDS             complement(30945..31631)
                     /locus_tag="SCK08_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200753.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31609..32103)
                     /locus_tag="SCK08_000843"
     CDS             complement(31609..32103)
                     /locus_tag="SCK08_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1200754.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32123..33343)
                     /locus_tag="SCK08_000844"
     CDS             complement(32123..33343)
                     /locus_tag="SCK08_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1200755.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33490..35325
                     /gene="lepA"
                     /locus_tag="SCK08_000845"
     CDS             33490..35325
                     /gene="lepA"
                     /locus_tag="SCK08_000845"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEG1200756.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        11847 a         6250 c         7613 g         9690 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat
    22441 aattatctag aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag
    22501 tgggtgactt tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact
    22561 tacccgttgc attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc
    22621 aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca
    22681 ccgtacgcct tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca
    22741 cgattgcggg tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc
    22801 aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac
    22861 gatcttggct gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata
    22921 aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa
    22981 tggagctgat gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat
    23041 ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc
    23101 aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc
    23161 catgacaatc agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct
    23221 agctgaagaa ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg
    23281 agtaatgatg acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat
    23341 cgatttgctg attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga
    23401 tcagccacaa gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc
    23461 acacacgaac atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg
    23521 tattgaagtg gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt
    23581 ttatgtacct gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg
    23641 tacacaaggt gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc
    23701 ggaagctggt gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc
    23761 aaaagttgag gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc
    23821 agctcatcct tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat
    23881 gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga
    23941 acaagtaaaa gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa
    24001 atcctctgtg aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc
    24061 aatcgcccaa agagcagatg tctatatcac gggagatatc tattatcata cggctcatga
    24121 tatgcaaagc gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa
    24181 gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt
    24241 tttcgtttct gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg
    24301 tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca
    24361 acaagcaaaa cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa
    24421 gaattagaag agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg
    24481 ggaacattac caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg
    24541 gatacagcag attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc
    24601 gagtctacga tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca
    24661 aatttgaaaa attatgaagg ccacacattg atcacaacag atggcagtac attactggga
    24721 gcagatgaca aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat
    24781 ccttctatcc ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc
    24841 ggagcggata aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt
    24901 ggtcctttag gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc
    24961 caagggaaaa atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta
    25021 gcaatcgatt ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac
    25081 gaaggtttct tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat
    25141 atcattcgcg atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc
    25201 caagaagagt tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa
    25261 tattacaaca tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa
    25321 gcaatgatcg agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt
    25381 tcaaaaatct cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg
    25441 cacggacgat tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta
    25501 aaaattgcag aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg
    25561 ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt
    25621 tgtcccgccc tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt
    25681 tttataacaa aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt
    25741 atgtaaaaga aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg
    25801 atgatgcgga attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag
    25861 gtggagacgg cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc
    25921 atgtcggatt acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc
    25981 tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg
    26041 gtcaagtcaa tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag
    26101 cagtttggat cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt
    26161 tttttaagtt attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc
    26221 attggaaagg aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta
    26281 caaattttga cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt
    26341 ccttagatat tttccgattt attttttatt tgcctagaat catgagaggc aaattctata
    26401 cgatccccgg cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat
    26461 ctgtgggcac tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg
    26521 tatcaaaagg attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt
    26581 taacagaaag ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac
    26641 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
    26701 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
    26761 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
    26821 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
    26881 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
    26941 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
    27001 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
    27061 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
    27121 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
    27181 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
    27241 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
    27301 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
    27361 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
    27421 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
    27481 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
    27541 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
    27601 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
    27661 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
    27721 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
    27781 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
    27841 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
    27901 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
    27961 tcaaatcaaa gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta
    28021 tatacataca atcaattttg tttttttaag atttatataa gtcgagaaaa aaagtcaaaa
    28081 aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc
    28141 aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa
    28201 tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac
    28261 atcaagatat cgacattgcc catgtatgga aaatcttttt acaatctaat cattttggtc
    28321 gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc
    28381 tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc
    28441 agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt
    28501 ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg
    28561 tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga
    28621 gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat
    28681 acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg
    28741 atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc
    28801 tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg
    28861 tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg
    28921 cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga
    28981 aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg
    29041 taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg
    29101 ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg
    29161 aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg
    29221 aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta
    29281 acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc
    29341 ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag
    29401 aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa
    29461 tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt
    29521 tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa
    29581 cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac
    29641 acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg
    29701 gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg
    29761 agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat
    29821 taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga
    29881 atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg
    29941 cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt
    30001 tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt
    30061 ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg
    30121 tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa
    30181 cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc
    30241 atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa
    30301 aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac
    30361 agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag
    30421 tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga
    30481 ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc
    30541 aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa
    30601 cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta
    30661 tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta
    30721 aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag
    30781 aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag
    30841 tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag
    30901 ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact
    30961 ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac
    31021 aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat
    31081 gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct
    31141 aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac
    31201 gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc
    31261 aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag
    31321 attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag
    31381 atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact
    31441 ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat
    31501 gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca
    31561 acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa
    31621 agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac
    31681 ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc
    31741 agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc
    31801 agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac
    31861 cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt
    31921 ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc
    31981 ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac
    32041 taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt
    32101 catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa
    32161 tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca
    32221 aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa
    32281 cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag
    32341 cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag
    32401 ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa
    32461 aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat
    32521 ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc
    32581 ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt
    32641 cagctaccca gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg
    32701 ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga
    32761 ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat
    32821 caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc
    32881 tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga
    32941 tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa
    33001 cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag
    33061 tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga
    33121 tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg
    33181 gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt
    33241 cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga
    33301 ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta
    33361 ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa
    33421 aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga
    33481 ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg
    33541 atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg
    33601 cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt
    33661 gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga
    33721 gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc
    33781 tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc
    33841 gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca
    33901 gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa
    33961 gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg
    34021 attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat
    34081 gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta
    34141 aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat
    34201 ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac
    34261 tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca
    34321 cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac
    34381 cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac
    34441 gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca
    34501 gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg
    34561 gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca
    34621 tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca
    34681 gattttcctg aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc
    34741 atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa
    34801 ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt
    34861 tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca
    34921 ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg
    34981 aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt
    35041 ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca
    35101 atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa
    35161 aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa
    35221 caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc
    35281 ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg gtttgtttga
    35341 aaacagtgta aaaaataaac catgtttaca tggatttgcc accggtttgc caccggtggt
//
LOCUS       DARBFD010000017        35060 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000017 DARBFD010000000
VERSION     DARBFD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.017"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            164..1309
                     /locus_tag="SCK08_000846"
     CDS             164..1309
                     /locus_tag="SCK08_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1200757.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="SCK08_000847"
     CDS             1446..1790
                     /locus_tag="SCK08_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEG1200758.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="SCK08_000848"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="SCK08_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEG1200759.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="SCK08_000849"
     CDS             complement(3485..4084)
                     /locus_tag="SCK08_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEG1200760.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="SCK08_000850"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="SCK08_000850"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEG1200761.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="SCK08_000851"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="SCK08_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEG1200762.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="SCK08_000852"
     CDS             7290..8408
                     /locus_tag="SCK08_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEG1200763.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="SCK08_000853"
     CDS             complement(8468..9760)
                     /locus_tag="SCK08_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1200764.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9892..10470
                     /locus_tag="SCK08_000854"
     CDS             9892..10470
                     /locus_tag="SCK08_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1200765.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="SCK08_000855"
     CDS             10599..10976
                     /locus_tag="SCK08_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEG1200766.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="SCK08_000856"
     CDS             10909..12981
                     /locus_tag="SCK08_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1200767.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="SCK08_000857"
     CDS             complement(13192..14139)
                     /locus_tag="SCK08_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1200768.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="SCK08_000858"
     CDS             14385..14849
                     /locus_tag="SCK08_000858"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEG1200769.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="SCK08_000859"
     CDS             complement(14911..15954)
                     /locus_tag="SCK08_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEG1200770.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16331..17296)
                     /locus_tag="SCK08_000860"
     CDS             complement(16331..17296)
                     /locus_tag="SCK08_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEG1200771.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17530..18375
                     /locus_tag="SCK08_000861"
     CDS             17530..18375
                     /locus_tag="SCK08_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1200772.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18566..19753
                     /locus_tag="SCK08_000862"
     CDS             18566..19753
                     /locus_tag="SCK08_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1200773.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19759..20115
                     /locus_tag="SCK08_000863"
     CDS             19759..20115
                     /locus_tag="SCK08_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEG1200774.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20117..20455
                     /locus_tag="SCK08_000864"
     CDS             20117..20455
                     /locus_tag="SCK08_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEG1200775.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    20531..20787
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20850..21884
                     /locus_tag="SCK08_000865"
     CDS             20850..21884
                     /locus_tag="SCK08_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1200776.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21877..22563
                     /locus_tag="SCK08_000866"
     CDS             21877..22563
                     /locus_tag="SCK08_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200777.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22587..23435
                     /locus_tag="SCK08_000867"
     CDS             22587..23435
                     /locus_tag="SCK08_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1200778.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23632..24405
                     /gene="sufC"
                     /locus_tag="SCK08_000868"
     CDS             23632..24405
                     /gene="sufC"
                     /locus_tag="SCK08_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEG1200779.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24418..25704
                     /gene="sufD"
                     /locus_tag="SCK08_000869"
     CDS             24418..25704
                     /gene="sufD"
                     /locus_tag="SCK08_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEG1200780.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25701..26936
                     /locus_tag="SCK08_000870"
     CDS             25701..26936
                     /locus_tag="SCK08_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1200781.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26923..27393
                     /locus_tag="SCK08_000871"
     CDS             26923..27393
                     /locus_tag="SCK08_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEG1200782.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27398..28789
                     /gene="sufB"
                     /locus_tag="SCK08_000872"
     CDS             27398..28789
                     /gene="sufB"
                     /locus_tag="SCK08_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEG1200783.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28884..30026)
                     /locus_tag="SCK08_000873"
     CDS             complement(28884..30026)
                     /locus_tag="SCK08_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1200784.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30065..30799)
                     /locus_tag="SCK08_000874"
     CDS             complement(30065..30799)
                     /locus_tag="SCK08_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1200785.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            31031..31306
                     /locus_tag="SCK08_000875"
     CDS             31031..31306
                     /locus_tag="SCK08_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1200786.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            31404..33401
                     /locus_tag="SCK08_000876"
     CDS             31404..33401
                     /locus_tag="SCK08_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200787.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(33477..33812)
                     /locus_tag="SCK08_000877"
     CDS             complement(33477..33812)
                     /locus_tag="SCK08_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1200788.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            33939..34154
                     /locus_tag="SCK08_000878"
     CDS             33939..34154
                     /locus_tag="SCK08_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1200789.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            34191..34553
                     /locus_tag="SCK08_000879"
     CDS             34191..34553
                     /locus_tag="SCK08_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEG1200790.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            34599..34925
                     /locus_tag="SCK08_000880"
     CDS             34599..34925
                     /locus_tag="SCK08_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200791.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        11545 a         6218 c         7348 g         9949 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    16081 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    16141 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    16201 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    16261 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    16321 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    16381 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    16441 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    16501 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    16561 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    16621 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    16681 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    16741 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    16801 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    16861 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    16921 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    16981 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    17041 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    17101 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    17161 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    17221 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    17281 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    17341 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    17401 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    17461 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    17521 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    17581 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    17641 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    17701 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    17761 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    17821 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    17881 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    17941 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    18001 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    18061 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    18121 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    18181 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    18241 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    18301 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    18361 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    18421 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    18481 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    18541 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    18601 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    18661 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    18721 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    18781 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    18841 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    18901 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    18961 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    19021 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    19081 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    19141 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    19201 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    19261 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    19321 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    19381 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    19441 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    19501 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    19561 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    19621 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    19681 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    19741 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    19801 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    19861 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    19921 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    19981 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    20041 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    20101 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    20161 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    20221 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    20281 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    20341 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    20401 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    20461 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    20521 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    20581 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    20641 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    20701 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    20761 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    20821 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    20881 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    20941 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    21001 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    21061 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    21121 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    21181 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    21241 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    21301 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    21361 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    21421 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    21481 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    21541 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    21601 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    21661 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    21721 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    21781 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    21841 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    21901 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    21961 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    22021 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    22081 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    22141 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    22201 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    22261 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    22321 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    22381 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    22441 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    22501 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    22561 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    22621 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    22681 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    22741 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    22801 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    22861 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    22921 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    22981 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    23041 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    23101 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    23161 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    23221 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    23281 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    23341 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    23401 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    23461 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    23521 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    23581 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    23641 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    23701 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    23761 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    23821 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    23881 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    23941 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    24001 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    24061 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    24121 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    24181 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    24241 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    24301 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    24361 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg
    24421 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    24481 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    24541 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    24601 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    24661 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    24721 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    24781 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    24841 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    24901 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    24961 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    25021 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    25081 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    25141 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    25201 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    25261 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    25321 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    25381 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    25441 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    25501 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    25561 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    25621 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    25681 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    25741 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    25801 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    25861 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    25921 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    25981 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    26041 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    26101 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    26161 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    26221 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    26281 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    26341 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    26401 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    26461 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    26521 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    26581 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    26641 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    26701 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    26761 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    26821 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    26881 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    26941 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    27001 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    27061 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    27121 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    27181 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    27241 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    27301 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    27361 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    27421 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    27481 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    27541 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    27601 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    27661 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    27721 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    27781 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    27841 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    27901 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    27961 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    28021 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    28081 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    28141 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    28201 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    28261 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    28321 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    28381 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    28441 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    28501 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    28561 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    28621 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    28681 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    28741 cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag
    28801 gagctagaac ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa
    28861 tgaccagatt ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct
    28921 gttttcttag gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt
    28981 tcatgcccta atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta
    29041 gcatgtgtat gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata
    29101 tatcttaacc aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa
    29161 acatgttgct ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt
    29221 aatttccagt tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa
    29281 gctcttgttt tcggagattg aattataact ttaccaaatt catctacagc aagagtttta
    29341 ttgacatgta gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca
    29401 ctctttctca tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca
    29461 aaatctttaa aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt
    29521 ttctcatact ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat
    29581 tgcattgcaa ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt
    29641 tcatatttac tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat
    29701 agtttaagct taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg
    29761 attgttgaag ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca
    29821 aatgtcatat caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca
    29881 taatcttttt gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca
    29941 ccagttcgtg gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc
    30001 tttttgtact ttgatatttt tgccattaaa tcatctcctc ttagtttaca aggagtactt
    30061 accactagat ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg
    30121 aaatcgtttg ttctagcttg tttatattat cagtaatttc tctcacagct tcttcgggat
    30181 aagacatgtc tttcaattga ataggtagcc attcatctac cattctttct agtagttctt
    30241 ctatactgct agtgaggaaa agaatgttat tgtcaaattc ttctacaata gcattgatga
    30301 ataattcttc tatttttaag atattgaaaa gactaatttt ttcattttta actttattac
    30361 aagtcttgtt agcaataaat tcttgcatct ctggttctaa tttggaatat ctatccagcc
    30421 atgtatctaa attagagtgt actggtggga actgttgacg aagatgctgg aacaggtttt
    30481 tatctggaaa tgttatacca tctagtacaa tgctatctct ataaagaata attcccaaaa
    30541 tatattcttc tacattgcca caaactaact cctctatact catatttccc aaatcagcaa
    30601 ttcgggataa tcgctctggt attggtaaat aggttccctt ctcccattcg tagatagttt
    30661 ttttgtcaat taattgaccg ttgttaatca taagaccaaa tgctggttgt gttaattttt
    30721 tactctttct tatagctttt atttttgttc cgagtatctt ttttcgttct aaataattta
    30781 ttggtggacg ttttatcata ttgaccacac ctcctaaaat tagtatacct caaaaaaggg
    30841 gtgttgcaaa taaattttac aaagctgttg acaccccttt tttatcgagg tattatgtgt
    30901 atgtcgatga actggtaatc attttttgtt tgattaatcg gttaataaca aaatactttt
    30961 attttgagca aaaaatacga agataattct tagaaaattt gttcaagcaa tcacgaaagg
    31021 agggggaaat atgtctctac aaatcattga tagcattgtt tctatacaag ttgaacaaca
    31081 agtgaaagag ataattacag tgctaaagcc tatgctagaa gacattattt ctggagtcac
    31141 tctagagtat atggactatc agcaggcaag tgaatatcta ggtgtatcag taggaacgat
    31201 tagaaaatat gtttctcaat acgggttacc tgttataaaa attgatacgg tcattagatt
    31261 taaaaaatct gatattgata gtttcatgga acggtataaa ggttgagtaa acaaataaga
    31321 ctaaaacata tagtaataaa gagtttgttt ttattatctg tatgttttag tctttaacag
    31381 aattttataa ggaggaaaca caaatggaaa catcaatgat taatattcca atttatgtag
    31441 atcaagaaaa attattagag aagccagtta caaaaaaaga aaaaactgat gtaagagata
    31501 ggtgtttaga taactataga gaagttacaa gagatgagtt tgtagagata ctaaattcat
    31561 cacaatcatt tattccaagc aagacaaaga ataaaggcaa tgacaaggag agttttgtgg
    31621 aaaccagagt aattattctg gatgtcgata acactgttaa agatgagaag gggaaggtag
    31681 tagatttgag taaagatgat agtagatact taagcgtaga aaaggtttta tctatcaatt
    31741 tagttcataa ttctgcgttt gcgatacaaa aaagtattcg gtactctgaa aatctagaaa
    31801 aatacaagat tgtctttcta ttaaaagaag caattactga ctatggtaaa atggttgctg
    31861 tttacgaata cttgaaaaag aacatccccg ggtgtgatga caatgtaaat gcttcgaata
    31921 gaatgttttt tggaggatat aagtcagatg cagtgataat aattaatgaa gacaacatgt
    31981 tagatattac tgagcttccg attgatttta ccatgacatg ttctaaaaat tgtcaaagtg
    32041 attcagggag agagtatgaa ctagtcaatg aaaccgattt tgttaagttg ataaaaaatg
    32101 acgacaaaga agaaatgaaa cagtggttta aagactgcct gtttgattcc tccgacatgt
    32161 cttttaatga gatttatgaa agattgttaa ccattgatat gaataaatta cttaagtcaa
    32221 gtaaaaactt aagatgtctg tttcacaacg atcataatcc ctctgctagt atttttacca
    32281 gtaaaactgg gcattccatt ttttattgtc attccagtaa ttgtgatatc agtgaaaatt
    32341 ttattggagt tgtgatgcta attttaaaga aagacagtcg ggtagaaaca tttaattggc
    32401 tattagagaa cctagacttg tatcctacac attttcgaaa attgaaagag gaatcaaaag
    32461 tactttttga cactcttgaa tcaatgaaag acaaactttt caggacaata aggaattatc
    32521 tagaggaaat gcgacgtgtc tatgatgtct tgctttctga tgttaatttt tttgatgata
    32581 gtaaggaaag tgtgacttgt attttgtcgg gggaacaatt agctaaaaaa atgagttctt
    32641 actatggtaa aacttatgac atagataaat gtaataagtt actatcactt atgactttta
    32701 taggattgat aaaaaaatta gatgatgaag atgttccttt gaagatgctc gaatacctaa
    32761 ataggttaaa ggaagagaaa gcgttaggaa actttaatca taagagaagc aatgtctatc
    32821 gattagacgt ttttaatgca acttctgtga ttcaaaaatt gaatgaagat atccttccac
    32881 ttctggaaaa aattcatttt acatatcaat atttttctta tgaatgggtt aagatatgtt
    32941 ttaacgaaaa agaggctaaa agagtatttc ctcaaaattc ccgatcagaa ctcagcaagg
    33001 aaaaacaatt gattttagat gaagcacatg aagttttaca gggcttatcg tggcaaggga
    33061 catatctaat gacagagaaa gaacttatta acactgtttg tgaacgccta cgtaatgttg
    33121 gggataacag cgtgaaaaat gctttaataa aaaacagagg ctactttgtt aaacaaggat
    33181 tcactcttga acgagcttct aaggatatta agaaactata caatgtgaaa gcaagtagta
    33241 gttttttaat atatttagcg aaaggggcag ggatagatga aacaataaaa gaattgatat
    33301 tatcaggagc tgggatagtt cctctgtatc aatttacaag tgtaacaaga gatgatggac
    33361 ggagagtaag gaagagaaaa gacccaattg tattaaacta gacaggttat ccatagctct
    33421 taatcttatt tcatctcaat acacccaccg ttaaagttcg gtgggttatt tatttttcaa
    33481 tctttcaata gctgattgcg ttcattttct tgccttttta atagcttcat atagtcaatt
    33541 gttcgaactg catcttcagt gctaagagaa cgatagagag ccaaaaactc ttcctcataa
    33601 tctacgacaa cattgtctag aaaatggctt acaggggttc ctagggctct agaaagagct
    33661 gttaagctat cgaggttagg ctctgttaca gaattttccc atcttgctac tgcctgttgt
    33721 gtagagaaga ttttttcagc taattctttt tgtgtccacc cgagagcttt tcgcttttct
    33781 ctaatgacct ttgcatattg tttctgtttc attaatacac ccccatctca gtatagtaca
    33841 acaagttgtt gtatttgtaa tggtatttga agttttttat attttatgag ttgaaataca
    33901 tcatattgtt gtatataata aaacaggagg ggagaatcat gaaaaaagta agtaatctca
    33961 gccgatacag aaaagaaaaa ggactttctc aaacggagtt agcaaaaaaa atgaatgtta
    34021 cacagcaatg tgtaagttcg tggcaaacag ggagaacgat tcctaaaccc tatcaaatga
    34081 agatgctttc tgagatatta agtgtaccaa tgaatgagct gttttctgat gtatttaata
    34141 aagtaaacag ttaactaata agcaattgaa tgattttagg aggagaagac atgtattgta
    34201 gaaaatgtgg caaagaaata acagatgaaa gtaagtattg ccagttttgt ggagcactac
    34261 aagaaacaga tgagacaacc ggaacagaca cagtaaagaa aagtactgag cagacaatta
    34321 atgaaatgaa ggaacaaatc gtacatggac aagagacagg gaagctgtat ttgattattg
    34381 gttggatttc tatggtagta tcgttagcat ttattcctgt tgtatttggt gctgttgctg
    34441 ttattatggg ctacttatat cgagaaaaag atgagaaaat gggtacgatt ttgatgatag
    34501 caggggtagc tggagcaatc tttggtgtac ttttagggat gtcaatgtac tgataagagg
    34561 gataagagca aattattttg gagataggag ataaaaagtt gaataaagta aatatgaaca
    34621 agaagtttgt cattataggt attgttgtct tggctattat aggtattatt ttttcaaaaa
    34681 tgacaggtac atctggatta gagaagtcat caaaaccatt ggtaacacag attttacaag
    34741 aacagtacaa cttaaatcga tcatgtgatg atgtgacgat tactcatgaa aatggagata
    34801 acacataccg ggctaaagct atattagaca atggaagtgc tatcaacata aatattgagt
    34861 actatccaaa gaaagaccgt atatatgtgg agataccata tgctgaggtg ttaatgttaa
    34921 attgatttaa atgtattatg cataccgtcc aaacaggaac tcccaaaggt gtaaattcac
    34981 tagggagttt ttatagtttc ctataaatta aatactaata caggtgacaa aaaaatatta
    35041 caatgattgt cgggaatttt
//
LOCUS       DARBFD010000018        34948 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000018 DARBFD010000000
VERSION     DARBFD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.018"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            606..764
                     /locus_tag="SCK08_000881"
     CDS             606..764
                     /locus_tag="SCK08_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1200792.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            836..2062
                     /locus_tag="SCK08_000882"
     CDS             836..2062
                     /locus_tag="SCK08_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1200793.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(2150..2542)
                     /gene="rpsI"
                     /locus_tag="SCK08_000883"
     CDS             complement(2150..2542)
                     /gene="rpsI"
                     /locus_tag="SCK08_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1200794.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2558..3001)
                     /gene="rplM"
                     /locus_tag="SCK08_000884"
     CDS             complement(2558..3001)
                     /gene="rplM"
                     /locus_tag="SCK08_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEG1200795.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3520..3987)
                     /locus_tag="SCK08_000885"
     CDS             complement(3520..3987)
                     /locus_tag="SCK08_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEG1200796.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4107..5645)
                     /locus_tag="SCK08_000886"
     CDS             complement(4107..5645)
                     /locus_tag="SCK08_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEG1200797.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5725..6411
                     /locus_tag="SCK08_000887"
     CDS             5725..6411
                     /locus_tag="SCK08_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEG1200798.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6612..7139)
                     /gene="msrA"
                     /locus_tag="SCK08_000888"
     CDS             complement(6612..7139)
                     /gene="msrA"
                     /locus_tag="SCK08_000888"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1200799.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7223..7900)
                     /gene="pgmB"
                     /locus_tag="SCK08_000889"
     CDS             complement(7223..7900)
                     /gene="pgmB"
                     /locus_tag="SCK08_000889"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1200800.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7897..9027)
                     /locus_tag="SCK08_000890"
     CDS             complement(7897..9027)
                     /locus_tag="SCK08_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1200801.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9027..11210)
                     /locus_tag="SCK08_000891"
     CDS             complement(9027..11210)
                     /locus_tag="SCK08_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1200802.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11207..12232)
                     /locus_tag="SCK08_000892"
     CDS             complement(11207..12232)
                     /locus_tag="SCK08_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1200803.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12277..13104)
                     /locus_tag="SCK08_000893"
     CDS             complement(12277..13104)
                     /locus_tag="SCK08_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1200804.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13101..14168)
                     /locus_tag="SCK08_000894"
     CDS             complement(13101..14168)
                     /locus_tag="SCK08_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEG1200805.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14194..15030)
                     /locus_tag="SCK08_000895"
     CDS             complement(14194..15030)
                     /locus_tag="SCK08_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1200806.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15027..15896)
                     /locus_tag="SCK08_000896"
     CDS             complement(15027..15896)
                     /locus_tag="SCK08_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1200807.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15966..17249)
                     /locus_tag="SCK08_000897"
     CDS             complement(15966..17249)
                     /locus_tag="SCK08_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1200808.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17263..18948)
                     /locus_tag="SCK08_000898"
     CDS             complement(17263..18948)
                     /locus_tag="SCK08_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEG1200809.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19223..20248
                     /locus_tag="SCK08_000899"
     CDS             19223..20248
                     /locus_tag="SCK08_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1200810.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20843..24517)
                     /gene="rpoC"
                     /locus_tag="SCK08_000900"
     CDS             complement(20843..24517)
                     /gene="rpoC"
                     /locus_tag="SCK08_000900"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEG1200811.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(24575..28192)
                     /gene="rpoB"
                     /locus_tag="SCK08_000901"
     CDS             complement(24575..28192)
                     /gene="rpoB"
                     /locus_tag="SCK08_000901"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1200812.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28920..29888
                     /locus_tag="SCK08_000902"
     CDS             28920..29888
                     /locus_tag="SCK08_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1200813.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29906..30811
                     /locus_tag="SCK08_000903"
     CDS             29906..30811
                     /locus_tag="SCK08_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1200814.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30808..31614
                     /locus_tag="SCK08_000904"
     CDS             30808..31614
                     /locus_tag="SCK08_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1200815.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31750..32739
                     /locus_tag="SCK08_000905"
     CDS             31750..32739
                     /locus_tag="SCK08_000905"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEG1200816.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33056..33580)
                     /locus_tag="SCK08_000906"
     CDS             complement(33056..33580)
                     /locus_tag="SCK08_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEG1200817.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33867..>34948
                     /gene="scm"
                     /locus_tag="SCK08_000907"
     CDS             33867..>34948
                     /gene="scm"
                     /locus_tag="SCK08_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG1200818.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDST"
BASE COUNT        10033 a         7373 c         6529 g        11013 t
ORIGIN      
        1 agattttttt ctgttgctga agattagaat gacaaaacta tttgaaacta cgcgtatagt
       61 aagtagcttt caaaatatat aaaaaaggag cgttctcaaa tgagaacatg ctcctaaaac
      121 tactttattg gcgtatggaa aaattatact tgaagtggat agcgggttga ataagttgat
      181 aagcatcaaa acatttataa aaattattca agactatgtc tgttccatat acaaatatga
      241 aaatcagtaa actccattta acttttttcg tagtgtgtct ttttatacat gtttatcgtt
      301 attaatagtg aataggaaaa aaactatttt tgatcattga caattaaata caatttttga
      361 ataacgatcc ctgctattcg agctagaaac tcaagtacgc catgacctga ttagcgacaa
      421 atacttagag atttcttttt gttctgggaa aacaaaatgc catgacaata gcagcaaata
      481 atgatacttc cgtaaggctc gccgtaaact ggggagaggt gaaattccta tgtgcttacc
      541 caaagcaatt caaaaatttc ataactacta ttttaggaga tgattttata gaatccagag
      601 tctacctgaa taaaaaacaa acctgtgcat ttctaggaat cgataataat actctagata
      661 agtggattcg tatatataat ttcccagtaa taagagttgg tggtgtagtt cgctttgacg
      721 tagaagatat taaagacttt atgaatacat ttaaaataac ttgaaatttt cttctcgccg
      781 catatactaa aattagtgaa atatactgcc ttactttttt taaggagtga ggattttgcc
      841 tacaaagctt tcaaatggga aatataaaac taatctgaga tatccaaaaa ggtttaaaga
      901 aataacagga attgcatcag aaaagtatca aaaaacattt ccaaataggc aattggcgat
      961 taaagccgaa aatgatatga agaagaaaat agaaaaagtt ttgagagagg agaatgcaaa
     1021 ctcattagaa ttaaaaggaa aaataacgtt taaaaaattt tacgaatcta aatggcttcc
     1081 tagatatgaa ttaggtcaaa caattagatc aaacaggcct cctagtgata taaccattag
     1141 taatacaaaa gatatatttc ggttgcatat tttacctatg tttggtgaat acgcaatgaa
     1201 ttatctaaat atcaatactg agataatatc agatgaatta accaaaaaat cgaaagaata
     1261 tgcaaatata aaaatcataa aaggatatgt tcgttccatg ttcgatattg cagaaatact
     1321 aaattatatc gaatttaatc gaactactaa aattattcaa agtatcactg cacctaaaaa
     1381 aaatgcttta gaagaaaaaa gaatacaaga aggtaagcaa gcactatcat caaaagaact
     1441 tactaattgg atagaagcag taaatgatga ttttaataat catttactta cttttcatga
     1501 ttacacactt tttatgatta ccctgtatct tggtgataga aaaagtgaga cttacgcttt
     1561 gcaatggaaa tatattgatt ttgaaaaaca aacggtccgt cttaaacata ctttagataa
     1621 atatcaaaga aaaaagttta caaaaggtcg aaaagatacc gtcattcaag taccagaagt
     1681 tgtaatgaca ttgctaagtg aatggaaatc ggtacaagct gatcaattgt tgaaattaaa
     1741 aattaagcaa acgttagatc agtatctctt tacttatact aaaccatctg gagaagtaaa
     1801 ttgtcctgtt catgccgatt acctaaacta tcgaatcaat tcaattaaaa gaaggcatcc
     1861 tgacttagtt catttatctc cacacaaact ccgtcatact tatgcaacta tagctagaca
     1921 aggtggagca gatatgaatc aaatttcaaa tgctttaaca cactcagata tttctacaac
     1981 aaagatatat gtgaacacac cagatatagt tgataaggct gtttttgaag cttttcagag
     2041 aggattgaat aagtgtgatt gatatgtgtt cttaaatgtg ttcttaaaaa caaaagaagc
     2101 actttccaaa taattgaaaa gtgccagaaa cgttgatata taaaggatat taacgttttg
     2161 agaattgtga agctttacgt gctttcttaa gacctggttt tttacgttcg accatacgtg
     2221 cgtcacgagt aagtaagcca gcacgtttaa gagctaaacg gaagtcagga tcaacttcta
     2281 gcaatgcacg agcgatacca tgacggatag ctcctgattg tccagcgtat cctccgccgt
     2341 ttacgttaac aaatacgtca tatgcgcctt ttgtttcagt aacagcgaaa ggttgattga
     2401 ttacttcacg caagtcagca tgtggaatgt attcttcaac atctttttta tttacagtaa
     2461 ttttgccagt tcctggtact agacgtacgc gagcaactgc gtttttacga cggcctgtgc
     2521 cgatatattg aacttgtgcc aatgaaattc cctcctatta gattaagttt gtgatgtcta
     2581 aaacttctgg ttgttgtgct gcatgtggat gttctgctcc accgtatacg ttcaattttg
     2641 taaattgttt gcggcctaaa gtgtttttag gaagcatacc ttttacagaa gtttcgatca
     2701 aacgacgaga atttttagca cgcaattcgc cagcagtgat tgatttcaat cctcctgggt
     2761 agttgctgtg acggtaataa attttgtcag tcgctttttt acctgttaat ttcactttgt
     2821 ctgcattgat gacgatgacg aaatcgccag tatccaaatg aggtgtgaaa gttggtttat
     2881 ttttaccacg tagtacagat gcaacaactg ttgaaagacg tcccaatgga acatcagttg
     2941 cgtctactac ataccattta cgttctactt cgcctggttt ggccatatat gttgtacgca
     3001 cgttattttc ctccaattta agttctgatg tttgacatac acttcgagtt tccggggctc
     3061 tttgtggggc aaacaatacc atttaacata ttacccgcta atctagtcaa tgtcaacgtt
     3121 tttctgcttg attgaaaaat ttatatgaaa ctttacttct tttctttctt ttatataaaa
     3181 gggtcgtttc ctgtcaaaat gaaaaacaaa aagaggctgt gacaaaagtc ttgtcacaac
     3241 cccttatttt gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc
     3301 ataaaacata agaagctgtt ttttaaaatt gtttcttgtg actcggagct gaacggcagg
     3361 cttacaacct cttggtaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
     3421 ggcaaaatat gaggaactat tttgacgttt gtttctctaa tactcaggtc aaaacgctga
     3481 tttcacaacc tctttttcgg tcaagttcaa caacagtttt tacgcgtcga tctctggcgc
     3541 tttgcccaaa tgagcttgga tcatccagat acgtttttct gtggctgttt tgaattcgat
     3601 aaagatatct tgtgttggat cgtcgccttc ttctccagaa acttcgattc ctttttggta
     3661 aagatctgct agataacgat agcctgcaac aagtgtttcc aaacgttctg gaatagtacg
     3721 atcccagcgg ccaatttcat cagggatttt tgtgttatct gcaaattcac gcaatgtaga
     3781 ataaggtgaa ccgtctaatg tgatcaagcg ttcggatact tcatctaact gagcgttcaa
     3841 atcatccatg aactcatcca tcattgggtg caatgtaagg aaacctggtc ctctcatgta
     3901 ccaatgtgtt tggtgaacaa caacagacat ttggcttaga tctgcaacta attgatttaa
     3961 tacttctttg gtttgttgga atttcatctt tcttgcctcc taacattttc ttacacttaa
     4021 aataatagta gactttcaat gaaaagtaca acaaaatcat ttacctgaga acgattttca
     4081 gttcgctttt ttatccgtaa agccaatcat ttttcagaat cagctaattc ttctcgcagt
     4141 tgagcaaatt gctgataagt ctgtgctaaa tgcttttgtg tcgaaacaaa taatggatag
     4201 attttctgat attttttatg atcagtttct tgtactccaa cttgctcttt caattctatc
     4261 aattcctgtg cttcactgat actttggatc tttccgatgc ttctccagcc taaaatcaca
     4321 gctcctaatg cggatgcttc tgtgacagta gggaaaacaa gcgggtaacc cagtacatca
     4381 gccatcatct gacgaaaaat cggagattga gcaaacccgc ctgtagcacg gatctctgtg
     4441 agctgatttt cctggcaaac tccttttaaa atccgataaa ggttgagaca gatcccttct
     4501 agaatcgcct tcatcatttc tttttctgta tgttgccgct gtaaaccgat aaaactgcca
     4561 aacgcatctg cgtcccaaag cggtgctcgc tcaccaagta aatagggatg aaaaatcaga
     4621 ccattggcac ccgctggcac aaaagcgata gccgaaagca gtgcatcata tggattttct
     4681 cctttttctt tagcagattg aacgacttcc tgtaaaaatg tatgccctgc ccaatcaaag
     4741 atgccagcac cattgctagt cgctcctccc actacccaat gtttctgatc cagaacataa
     4801 caaaacgtct ccatttctgg atggaactga ggctgagtag taataaaacg aagtgctccg
     4861 ctcgttccta cggtcaaagc ggcgatcgta tcaccaaaag cacctaaccc tagattagaa
     4921 agcggcccgt ctgcccctcc caaaacaaat gatatatcat ttggcaaacc tagttctgct
     4981 gctgtttttg atggtagacc tgttacttga taggtggaag gaaagacttg cggcaattgc
     5041 tctattcgaa tgttcaagta gtcaagcact tcttctgccc attggaattc atggatatca
     5101 aaatatccag ttcctgaagc tgttccgtag tcgctgatca gttcgccaaa caatctataa
     5161 aagatatagt ctttgatacc gacaaaataa gcagtttttt tgaataattc aggttggctg
     5221 ttttgcagcc acttgatttt gttcaatggt gtcattggat gaatcggcgt cccagtctgc
     5281 cgataaaatt ctttgccaag cacggtatat ttcagttttt ctgcttcgtc cgctgctcgg
     5341 ttatctgccc aagtgatcat tcgagataac ggtgttttct cttcatccaa caatatcagg
     5401 ccatgcatcg cagaggagaa ggagacaaat tgaatgattg tgtctgaagg caactgttta
     5461 attacttctt ttataacggt gcagacacct tgaaagatca attcctgatc ttgttctgcc
     5521 atcccttgtg tttcttggat caaagggtag cgaaaataag cggatgctgc ttcttgggct
     5581 tcatctgtga aagcaactgc tttggtttgt gtggttccga tatctaatcc gattgaaacg
     5641 ttcatttttt cacctcaaat tttatgtatg tacaaaaatt tagtttaagc ttgcgtatct
     5701 tggttttgaa aagggggaga tggcgtgtta ctttatttta tcattggttg ctgtatcgga
     5761 tcatttttat gtttaaccgc ccagcggatt ccgttaggtc actctattat ttatcctaga
     5821 tctcattgtg tcaaatgctg tcgttcgctt tcttggtatg aattgattcc gattctctcg
     5881 atcatcgtcc aacgatttcg ctgccgctat tgcaggtgtc ggttgccctt ctactatttg
     5941 ttggcagaag ccttatgtgg tggtcttttt gcctggttct ttactttttc ttccgcaaga
     6001 aatttttcga cgttcatatg gatgctttcg gctttgctat tttcgttgat ggatttattt
     6061 tacttcatgg tccattctca cacgctattc tgttcttggg caattttatg gattttttgg
     6121 ttgcaggccg gcgtcttcca atggcaaagt gtgctgttaa cgtttatcgt aggcggtgtg
     6181 tgccttcgct atggtcaatc ctatctcgga gctggcgata ctctgttgat gctttcttgg
     6241 agtggtggct tgtctctaga agagttgctt cagatcttgt ttcttgctag tctgtttgga
     6301 atatccgttt tttctgttta ctttatctgt cataaaaaaa agttggaaaa gctgcctttc
     6361 attccttttt tgagcagtgc gttgctgatt gtccttcatc ttaggtattg aaaaaaggtt
     6421 gtgacaaacg acttgtcaca acctctgata tatagtgttc tcttaccaaa tccttgtgac
     6481 ttggagctag gcgacaggtt cacaacctct ccactaaatg gtgttcaatc agctgaccct
     6541 cagtattagc tcaaaatgct ggttttacaa cctctgatat acggtgttcc ataagtagct
     6601 ttttcggctt cttatttctt gacgccccaa tgtcgacgaa ggaaacctgc acgtccagaa
     6661 ccttctcgat aaagattgta atggagcttg tttttcttgt aataatcctg atggtaatct
     6721 tctgcaggat aaaattcgct tcttggttcg atttttgtca cgattggctt atcgaatcgc
     6781 ccgctgtctt gcaatgcttg ttttgaacgc tctgcaatat ttcgttcttc ttcatcatta
     6841 tagtagatca ctggacgata agaatcaccg cgatcagcga actgtcctaa tgcgtcagtc
     6901 ggatcggttt gctgccaata gatttccacc aactgatcat aagaaataac ttctggatca
     6961 tattcgatct ctactgcttc tgtatgccct gttgtgcctg ttgttacttg ttcataagtt
     7021 ggatttggta catgcccgcc agtataaccg gatgtcacag aaatgatccc tggctgctct
     7081 tcaaatggtt tgaccataca ccaaaagcat ccgcctgcga aaactgcttt ttttgtcata
     7141 taaaatcccc cttttttctc atcatacctt gatttccaaa aggaggataa cgttttgctt
     7201 gtgacagaaa aaacggaaac tattatatac gtgatacggc ttctgataaa tgccaaaacg
     7261 aacgtacttg atcaaaaacg gttaaatcag gaatcacata ttcagcagaa ggaagattta
     7321 tgttttcacc tattcccaat acatgcatgt taccagccaa agctgcttga caaccagctt
     7381 ctgaatcttc aatgaccaag catgcatttg gcggtacatt caattgctgt gctcctttga
     7441 gaaaaacttc tggatcaggt tttgcttttg atacttgcgt gccgtcaata aggacatcaa
     7501 aataacttgt caaattcgtt ttttctaaaa tgaggcgtgc atttttgctt gctgatccca
     7561 atccgatctt gatgtttgtt tttttcaagt actcaagaac agctattgca ccaggcaaaa
     7621 cggatgtttc atccattttt tgaatcgctt gaagatatag attgtttttt tgctcagcaa
     7681 aggctgattt ctctttttct gagaatttct gttccatttt tccaaatgac agcaatcgct
     7741 ctagtgactc ggttctggat attcctttca gttgctcgtt ttctttctca gtgaatggaa
     7801 tggacagctg gttagctaaa accagccacg cttcgtaatg ataacgagct gtgtctacaa
     7861 gaacgccatc taaatcgaat agtgctgctt tgattttcat cgattacctc cttgaaaatg
     7921 aacgacttga agtaaagatg cgatgttttt agctgtgcga tgaagattat cagtggcttg
     7981 ctgtaaagct tcttgtaaag gcacagctgc aggaaggatc ggaaaaacgg ccgaaatgcc
     8041 aacatctgct gctttttcgc ttcctttacc aacacttccg caaatagcta tcacaggaac
     8101 gccctctggt actcttctag caatcccaac tggcgctttt ccttttagac tttgtccatc
     8161 tagacgtcct tcgcctacga ttacgaggtc tgcttgctgt acccgtttat cgaactccag
     8221 taaatccaat acgtagtcga tgccagagac tattttccct gaagcaaatg ctaacagtcc
     8281 tgcacccatt ccaccaccag cactactgcc cgatgcctcg attacttgag gggcaaatct
     8341 catataaaaa tcatgcatgg cttggtcagc cactgctaat tcattttctt tcaagccttt
     8401 ttgtggtccg aaaacaaaag aagcgccttc ttttccgcat aaaagattag taacatcagt
     8461 aatgacagtg attttggctt taatcagctc aataggcacg ttcgttttat caactgtagc
     8521 aattttacct agattttctc ccagtgcttc tactttttga ttatcttgat cgtaaaaagc
     8581 ataacctaga ccttctgcca tgccgatgcc gccatcgttg gtactacttc cgccaacgcc
     8641 gatgatgatg tgtcgaacac cttgttgcaa caattgtcgt atcagttctc ctactcctcg
     8701 cgtggataac ctaagtggct gttgttggtt tagtgggaca tctttcaatc cgacgatttc
     8761 tgccatttca aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt tgtttttgcc
     8821 aaatgcatta gtaacgactg ctgtttgccg cttcaacctt aaactgtctg ctaatgatga
     8881 aaccgttcct tctcctccat cgcctatagg tagcagatca tattcagcct gaacaaaaac
     8941 ttcttgaaaa cctgattgaa tggcttgtgc tgcctctaag gctgtcagac tttctttgaa
     9001 agaatctggc gcaatcagaa tacgcattat tttcccctcc gttccaagat cagttctttt
     9061 tccagctggt attggtctcc attgacaaaa ataggtaatg tttctttcgt ctcttttttt
     9121 attactattt gtttttttgt caaaacaaat agcaacctgt gatttttcca aataaaagga
     9181 aaagaaattt tttcccatgc tgaaggaagt cttggagaaa tagaaagagt tccattcgtt
     9241 actgtaatac ctgaaaaacc aaatataact gctagccata gtgctcctaa tgaagcagca
     9301 tgaattccgt catctgatga atgcggatct ggtcctaaat caatcaggca cgcttcttgg
     9361 aataaatgat acgcccattc catttcttgg atacggcaag cctctatggc gtgaatcgct
     9421 ttactcaacg atgagtcgtg gattgttttg ttttcatagt aatgcaaatt cttagaaacg
     9481 attggttgag aaaaatactc aggtaataag tataacagca tgacaagatc tgcttgcttg
     9541 ctgacttgga gttcatttac ttcgctacgg gcataatcaa gtaaaataga ctggctgcca
     9601 ggacgttctt tgtaagtggt caaatcaata actggcttac tgagatacgt gtcatcttgc
     9661 ggaatcagct gttctttgtt aggcactggc aaatataact tatctaaaaa attttgacat
     9721 tctttcaaaa aaagcggatc gccagcttga tatttaacag ctaaggccac attataatgc
     9781 gccagatagt tcgtatatgc attattgtcg atatattctg tgtattcatc tggaccaatg
     9841 actctgtgta tctctaaatg atcatcgact tttacagctc tgctcagcca aaaagctgct
     9901 gtttcatgaa tgagcgtttt tccctttgtt tctaaaaacg attgatcctc tgttgcttgg
     9961 acatactctt gaacggcata agcaaggtct gcactgatat gatgttctgc ggcagctgaa
    10021 gcaacttctt gtcttgttcc ggttttgata ttcaatgcag caaatttggg tgtctcttcc
    10081 tgaccagtgt gtgcgctttc ccatgggaaa agtgcacctt gatagtgata atctcttgct
    10141 ttttgccgtg cgccttctaa tcgatcataa cggtacagca gcaaattctt tgctacttca
    10201 ggaaactgat ataaaaagaa aggcaggata aaaatttccg tatcccagaa aacatgccct
    10261 ttataacctt cgccagttag tcccttcgcc cctacactaa cacttggatc attgcgggga
    10321 gtcataatct gtaaatggta agcagcgaag tccatcgcta actgatcgaa agaagaatcg
    10381 ccttggatcg ttatccgatg ctgatgccaa aaatctgtcc aataagacat cgactgctct
    10441 aaaatttctg tataactaac gcttttctgg gcttgggcaa tacttttctt aaacgggtct
    10501 gtttgctgaa gagaggtaga gagcacgatg atcttctcca gcgtaaagga cgtatttttt
    10561 ctgatctttt ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa tacacctgct
    10621 ttattgaatt tagctgcaat cccaatatca attttacttt ctgttgtctg gtaggatgca
    10681 ctgatcaaat cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg tgtaccaaaa
    10741 ttggtttgct gtccatcgat acccgttttc acatctactt ggcagtcttc ggtcaacggc
    10801 ttgatgacca ctttcagggc tgcctgatgg agattatttt gatcagcaac acgacaagta
    10861 atgacttcca ctttttttcc ttgagcagtt tcaatgagcg catagcgttt aagctctccc
    10921 atttctaaat ctagtgcccg ctcgtatgca agaactttca tcgtctcaag agaaaaatgt
    10981 tcaccgtcga tttttatttc ccatctgatg acatctggga gattcactaa ctcggctgat
    11041 tctgctccta aaggtcggtt atatacacca gctgcaaaca ttccccttac ttgattccga
    11101 tagtcttcct cctggcttcc tctgatacca aaaaatccat ttccaatggt catctgactc
    11161 gcatacttat tgatatactc taaatcaaac tgtttttctt ctactttcat aaggtaataa
    11221 cttctcctgt ttctgccgaa cgatataaat tttccaccag attctggatg acatatcctt
    11281 gccagccatc tgcaatcatc tgagtctggt caccgagcgc ccgattcaca aaatgagcca
    11341 tacttagttc atgtgggtca ttaactggcg tggtcttttg taaggaaacc agctttccat
    11401 ctacatcttc gaatagttcg cctggataca gattagcacc tgcctgatcg ccacaaaaag
    11461 ccacattcag tttgtcttct ctgatattca aagcaaacga agtattcagt tggatgatct
    11521 tgccgttttc taattccagt gtgccaaaaa ttgcatcttc tactgtgaat ttgcttggat
    11581 cccatttccc gaatttccct gaattttttt tcgtaccaat tttttggaag ctgtacgccc
    11641 gaacgctttt tactttagga aatccagtaa tatacatcgc tgtatcaagc atgtgaatgc
    11701 cataatcgat caaaggcccg cctccttgaa gttctttgtc gataaacgtt ccccagcctg
    11761 gaacaccaga ccgacgaagc gcattcgctt ctacgtaata aatatccccg agaacatcaa
    11821 gatgattctt gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca taagccaaaa
    11881 tctgcccgtt tttatcggct gtctctgcca tctttcttgc ttgatcagct gtcatagctg
    11941 gtggtttctc gcacatcact gaagcattag cttcgagggc agccaataca tgttcgaagt
    12001 gaaagcgatt gggtgtacag acactcacga tatcgggaga aatctcattc agcatttgtg
    12061 taactgatgt atacgcatga gaaaatcctg tacgtcggca aaagtcttct gctcgctggc
    12121 tgactggatc aaccacagcg gccaattcta agacatctga tcttttttga taataagcag
    12181 catgtacttt ttctgctact tgtccagcgc ctatgattgc tactttcttc atttatttct
    12241 ctctcctttg cttatttact tattgttatt ctttctctat tctttctcta aatcatttaa
    12301 atgacgcgct gtttccagac agtttttcaa gtattgcaat gattcctgat aaattttcac
    12361 ttcattttct cctcgcaaac gacattcata taccatataa ccttcatact cattttgaag
    12421 tagcgtttga aaatggcgag taaaatcaat tgcgccagat cctggttggt agcgatgatt
    12481 atctgctaaa tggatatgac ccacatactc tttttgcgta gtcaatgctt ttgaaatatc
    12541 atcttcttca atattcatat gataaaaatc agcgatgatc tgcacatgtt tcaattgatt
    12601 agagtcaatg taatgacgcg catccgctaa caaattgatc atatggtctt ggtatcggtt
    12661 caaaggctcc aaataaataa ccgtacctgt ttcttcggct actttatcta aataacgaag
    12721 tgattcgctg actgctttat agtctccttc tttgcttcgg ggtgaaacca tcggcggtaa
    12781 tcgataagtg aacattcccc aagctgctgg gataacgatt cctttgcctc caacctcgct
    12841 tagtgcacgc aaaatctcag caatttgacg tacaccatta agacgccgtt cttcgataaa
    12901 gtcaccgatc cacccatcgt agccgttaca agctgtcact actgggatcc ctgtttcttt
    12961 gattgcctgt ttgactgtgt cgatattttc tattaacaac ttaccgtcga tttcgtattc
    13021 ttcaaatcca cagtctttga taaatctaaa tttttctaaa atgtctcttg ggaaaaatgc
    13081 ttgatcttgc gtaccgaatt tcattgttct cctcctcgga cttgaacgcc cattttgata
    13141 ctttcttctg ggtgctggtc tacataacgc atgtaagctt ctgcgctttc tgcaaatggc
    13201 acaactggat caatgatttc ttcacaatcc aagtaaccgt tcatcaataa ttcccaacat
    13261 gtgtcctcga ttcgtttacg gttccatctt gggtaatctg ggtttggttc actagatgct
    13321 cgagcaaaga cgatttttgc attattgaaa tgtgcttcgc gaccaagatt aaacccagct
    13381 ggaaaaggtt tagcaaaggc aacataagaa atgatcccgc catagccgat ccctattaaa
    13441 gaagcttgta atgcggcagc actgccactc gtttcaataa ctgaatctac tccacgttta
    13501 tctgtcaatt cctttagacg aaaaccgaca tctgctttca taggatctag cgtgtaatcg
    13561 gcaccgtgtt tttctgctaa ttctctacgc tgcgctatgg gatcgatccc aacgatggta
    13621 gtagcacctg cttttttagc taattgaatc gcaatttgcc cgatagcgcc taaaccgaag
    13681 attgctacag catcacctgc tcgaacatgt gcttcccgga taccacctaa agcgaattga
    13741 gcaggatcgt aacatactgc atttttccat tgatccaacc gatcaagttt acgtagttta
    13801 taattatcaa caccgtttgt aatcagcgtt tcacgaatgg gtccataagt gcagactaac
    13861 tctccttttt gatactctgt caccaaactg cccgtttctt cgatcgtacc gacaatcata
    13921 tttcctaatt gaaattcgcc aaataccaca ccgcgttggc ttccttcttc tcttggcaag
    13981 aataggcgcc attcttctga aaattcttca tcgataaatg gacttaatcc tctaaagtcg
    14041 acaatttctg ttcctggttt tggagtagcg aaaattactc ggatacgtac ttcgttcttt
    14101 tcgatctcac ggtcttcgta atctttgaat ccggctattc ttggttctaa tgcaactaat
    14161 tttttcatct tttctttcct ctttcttttc tttttatcct tttacgccgc cttcgactaa
    14221 atcaccttta ataaatcgtt cagataacgc atacataatg atgactggta aagcggtcac
    14281 caatgaagcg gccatcattc gtccccaaat gtagtctggt gtactgaata atgtgttcaa
    14341 accgattggt aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa ataaataatc
    14401 attccacgaa accataaagc aataaacgaa gacagaaatg attccagaaa tagccaatgg
    14461 tataatgatc cgaataatga tctggatccg gctaagacca tcgatcattg ctgcttcttc
    14521 aatatcagtc ggaatcgtgt caaaataact ttttagcatg aatatcgcag taggaagtgt
    14581 ttgaacgacc atcgtgataa tcagtgctgt attggtatca tacaaaccta gaccggaaat
    14641 gattttaaat aatggaacaa cgatcaaaat accggaaaac atataaacgg tgtaaaagct
    14701 agcattgatt ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg ccccgctgat
    14761 cccaatcact actgcagtta cggaggctat caaggagatc actaaactat ttttgaaata
    14821 catgatgaat ggaaaaatct cagcattgaa aatatcctga taatgttcaa aagtccattc
    14881 tttcggaaaa atagtttgtg tcgttgcaat tgcttcttgt gaatccttga atgatgtcat
    14941 cagcatgatc aaaaatggaa acaaattgac agctaatagc aacaaaatcg tcgcgtaaaa
    15001 taagattttc ctgctctttt tatttttcat ttcaagtcca ccttctttcg tgagagcaag
    15061 acgaccgctg caataatgat gaacaataga atcgatatag ctgctgcttt tccaagatca
    15121 ttgaacgcaa aggctgtctg atacaaataa acgcctaaga tattgacttt attcgtcagt
    15181 agatagacat ctgtaaacat atagaacatc cagatagatc gtaaaacaat cacagttgct
    15241 agtactggtt tgattgccgg cagtgtcacg atcttgaact tctgccaagc atttgcaccg
    15301 tccatttcag ctgcttcata aagcgttttg tcgactgttt gcaaaatagc caaaaatgaa
    15361 ataaacgcat acggatagta tctccagata gcaaacaaaa cgaccataaa gaagctagca
    15421 actggctggt cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc tactaacaga
    15481 tcattgacga ttccataact attattgaac atatatttcc aagcaaaaat caaagaaata
    15541 gatggcgtaa cataacttaa taaaatcagt gaacgcaccg ttttcctaaa tctgaacttg
    15601 cggttaagca aaatagctac ggctaatcct aaagctgtac tgcctgctac aactaagaat
    15661 gtgtaagcaa ctgttaaacc taaagaacga taaaattcac tatcacttaa aatttcaaga
    15721 taattgctta atccgataaa aacggcgtct gagttaggat tcagcggcat atcgaaaaaa
    15781 ctgatctgaa tattggaaag cattggaatc agcactaaaa cagctaataa aagcatgctt
    15841 ggtgctaaaa gtcccatggc taattttaag tcagaatgtt tccttggacg attcattgaa
    15901 acctccccct gataatctgc caatagctat ctactttttt cgaacaccat tggcagaact
    15961 tcttgttatt tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc ttcgtttccg
    16021 acagttactt ggttcactgc ttttccgatc acacctgagc ttgtgatatc tcccattttc
    16081 gtgaaattct tttccccgac tagtccaaag acttgtactt catcaaaagc actagcaatc
    16141 tcagatggca gctcgccaaa agacttgatg acttcatttt ctttgtaacc atccagttcg
    16201 actacttgtt tattcactgg ttgagcacca ccaggagaca tcaagaccca cttttccatg
    16261 tttttcggtt gagataagaa agctgtgaat gcttcagcag cctctttttg tttctcgtct
    16321 agaccagaag aaatcgtcaa tccagatact gtgccataga cagcttgatc tttttgtgtt
    16381 gggatagcaa aaccgatatt ttctgagttc cccgcttcat aaacagaagg caaaatgtaa
    16441 gttgaataga ttgccattgg tgctgttcca ttcatgaaag catctttgat ttctgtcaca
    16501 tcattagagc ctggcatcgt gtattgggat aagttttgat aaaacgacaa tgcttctctc
    16561 atcttttccg tatcgatcgt tacctcgcct tcatcatcca aaacatttgc tccatttgac
    16621 aaagcaaatt gagagaacgc ttgctcactc attgttcctt cagcagtagg catagcaatt
    16681 ccatattgtt tattctcttt atctgtgaaa tgttgagcaa tttttaaaat atcatcccaa
    16741 ttttctggtt ctgaaaaacc agcttctgat aatttttctt tgtcatacca aataccttga
    16801 acccagccgc taattggtgc agcaatatag ctttttccat cttctgaacg aacaaggttt
    16861 ttggctccgt cataataatt atcttctcca gcttcctcga taacgttttg gactgccgtc
    16921 tgatcaatca gttcatcttt atccatcact ttagcaaaat cctgactgac ttccattact
    16981 tctggtaatt taccggaacg agctaacgta acgatttttg tattgaacgc tgattcttct
    17041 accggcactt gcttgatttt gatgtctgga ttttcttttt caaaatcagc aaccagttca
    17101 ttgatcacac ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat cgttgtctgt
    17161 tgtgattcgt tagagctttg actttcacca caagcagtaa gaagaaatcc agtagctagc
    17221 agacaggtag taacaataat ttttttcatt tttttccacc tcttatttta ttattttcag
    17281 ccacatatat tcatacggct ggaatatcaa ctttcgttct tgactgcaat ggataatttg
    17341 tccagaaaac aggtctaggt aatctccttt tggtaaaaga taacctgctg attgattgga
    17401 aaaattatgg ataactaaga ctttctcttc cttcgcttcg cgaataaaac tgataaaaga
    17461 atctgctgag tccagtaagt aaatcaaact attcggatgg aaggcttttt ctttttttcg
    17521 aatagatagc atctgggtca attcaccata tactgctgtt cttaatggat ctgtctgcaa
    17581 ctctttctca atatctgcta acaaaaattt ttgccgattg atttttcgat tctctcctgt
    17641 tttttctacg cctttgtaat catttcgact tcccagcaca ctttgaatat aaattgccgg
    17701 cacgcctggt aacgttacca aaaatgaatg agcagctaaa aagcgtttgc ttctccattc
    17761 cgtcgtatct gttggagaac ttaacgcatc tagatacgta gcgtttatct catatggact
    17821 ggtcgttcca tctgggtttt tcttgtagtt tactcgggca ccctctttct tcagctgttc
    17881 cactaattca aggatcgttt tttccggcag aatacctcgt accggattca atccgatacc
    17941 gtcatgggag gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat cgatggtttt
    18001 taaccattct ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg tcaaaggtgg
    18061 tagcgaaaac tgatagacca tctgtgcttc attctcgcca ttcccaaaat aactgatatt
    18121 atctttgtgt ggcacatttg tctcagtgat caataccgtt cctggagctg cttcatcagc
    18181 gattgcacgg aataattgga tgattttatg cgtttctggc aggtgtatac aagaagttcc
    18241 tagttctttc cataaaaatc ccacagcgtc caagcgaata aatgaagcgc ctttttctaa
    18301 gtaaaacaaa agaatatcga tcattttcaa taaaacttct ggatttttaa aattcaagtc
    18361 tacttgatct tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt tgtcaatgaa
    18421 cgttgtaagt aacgggctgg tccttggtct agtcacgctg cttgtatctg tatccagtgg
    18481 caaatcaata aaaaaatctt tgtacgtatc attcaagttt aaatattcct gaaaccaagt
    18541 actttcggca gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt cgcttctcat
    18601 ttcttctata tcttcccatg tgcccaaacg gtcgttgatt gcctgataat ttacaacgga
    18661 gaaaccatca tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc taaatgtatc
    18721 tgccaaatac ttttgataaa atttcttaaa tgtacgcaaa gttggttgct gctcttcaaa
    18781 aaattgatcc ccataagtaa tcaagaaaat atcttcttcg tcccaatgca gtttgcctgc
    18841 ctggcctttc ttctgctgat aaacactgac tttttgttca atcatagagg cggttttctc
    18901 agacaaatct gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg gattcccctt
    18961 tcttttttgg gagcgttccc aacgatttga tcatattatg gaaaacgctt actgtcaata
    19021 gcattttttt aaataatttt gatatacaca aaaatacaaa taagaaagct tatgtatcaa
    19081 taattaatag cttcttaaaa aaatatggtc tctcatgatc atgcaagcta ttcctaaatt
    19141 ttcaaaatca tagacaaaaa agaatgtcta ttgtactatg gaattattct aaaacgctcc
    19201 caaaaaggag gtgcatataa aattgaaacc tactatttat gacattgcga aaaaagctaa
    19261 tgtctcaaaa tcaactgtct ccagagtatt aaataaccag ccaaatatct ctgataaagc
    19321 aaaagcaagt gtacttcaag caatcaaaga attgaattat gaaccgagca aaattgcaag
    19381 aggactgtca ggaaatggtt ttgacgcgat tctagcatta tctcatcgta cttctcatac
    19441 gacctatggc aatccttttt tttcagatgt tattcaatcc atttctgccg tttctgaaca
    19501 aaataacttc gacttgattc tacaaacggc aaaaacacca gaagaagagc tagaaaaatg
    19561 cgcgaaaaag atccaagaaa aaatgatccg aggaattatt attttaagct ctacgatgga
    19621 tgaaacactt ctttttgaat tggatcaata taatattcct attgtggtaa tcggaaaagt
    19681 cggaaacgac tacacgaaca tctactcagt ggacacggat aatttccagg atagttacga
    19741 tttggtggaa acactgatca agaatggaca ccgtcatatc ggctgtctcc atgctccgac
    19801 agaagtttat gtatccaatg atcgtctcaa tggttaccgt caatgcttat ttgatcacca
    19861 cctagatatc cgtaatgaat ggattataga cggcggcttt cgcttagaag atagcatggc
    19921 agcaatgaat gatttaatga agctacgaga aagaccaact gcgatttttt ctaccgatgc
    19981 attaaaatca ttgagtattt acaaagtggc agaacaatct ggaaagcaga tacctgatga
    20041 tctttcgatc gcaggattca gtgatccctt attgtcctcc ctattttcgc cacagttgac
    20101 tcggatagac attccgacaa atcagttagg aatcaaagcc acagaacttt tattccaact
    20161 aatccaccac caataccctg aagaaaaaag aatcattctt tcaaccgaga ttactctcac
    20221 agattcgatc caacaacaag aaatgtagaa cagaattata aaagaacaga aacactgagg
    20281 ataagtcagt tactctgttc ttttttcaat cactcatccc tcgttacccc ctcccggtat
    20341 tttagcgaat ttttcttatt ttccatctgt tttagatagc tgtggataac tataatgttt
    20401 gtgttaaact attttttcca cagggaagaa aaaactattt ttaaccacgt tgtgtataag
    20461 tttttatagt tcgttttttt aaacacattt acccttattt caatccatgt tatttgggga
    20521 tacttatctc taaaacataa atagaataac agacatgtct actggttttg ttttattcga
    20581 ggaattattc aataatagct agcaaaatat taaaaataac cgctctatct tcccatcaat
    20641 ttattttgca ttcaaaaaat gatctatctt tacttttgtt gattgacttc tcttggaaga
    20701 tgatcgttcc gataccttaa ttggatacga accgatgttg ttaagatcct ttcccacttt
    20761 tcttctagtt agacaaatta ttttagacaa accaaaaaag aggttgtgac aataatttgt
    20821 cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct tctgcagcca
    20881 tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg acttcttttg
    20941 gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta cctataataa
    21001 cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca tccgtcaaga
    21061 cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct aatgatgctt
    21121 ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag actagcgtat
    21181 tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag atatctgtat
    21241 cacctggatc cattacacga actttacgca gcatttgacg aaccattact tcgatatgct
    21301 tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga agcaagtagt
    21361 tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt gatccttctg
    21421 tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt gctgtataag
    21481 gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg gtacgtgttg
    21541 caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact gcttgccctt
    21601 tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg atatcgtctc
    21661 cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt tcaccgattg
    21721 attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca gttgcaaggt
    21781 tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag acagaacgga
    21841 ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt
    21901 ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta cgggtgtaac
    21961 gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg atggcttgga
    22021 tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct tgggccacgt
    22081 caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta tcggtcatcc
    22141 ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct tcacggaagt
    22201 tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa ccacgcattc
    22261 cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac atcatgaaga
    22321 ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct tttgcagcat
    22381 tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca cgacggaatt
    22441 gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt ttttcattca
    22501 gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt ttgtacccta
    22561 agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt ttgaagactt
    22621 ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct tgctctttga
    22681 tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt tggacggtca
    22741 agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc tcgttgaaga
    22801 tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta aatggttttt
    22861 cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc
    22921 atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg cgtccttctt
    22981 cttccattgt caagtagtag ttacctagaa ccatatcttg agaaggagta acaactggtt
    23041 taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt gcttctgctt
    23101 gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa tcggcattat
    23161 aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga
    23221 atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact gggtgttcct
    23281 tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt ttacgtttcg
    23341 catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg aatggtttga
    23401 ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag aaaggtccaa
    23461 caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt tgacggaaac
    23521 gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta cctggtccag
    23581 taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct tgcagcatac
    23641 gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt ttcaaacggt
    23701 tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca aaacgtccac
    23761 cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg tccataacca
    23821 tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa cggcggattg
    23881 cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg gctgcttctg
    23941 catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata gcagcttgga
    24001 attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac aattgttttt
    24061 tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa atgatttctt
    24121 ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc cctttaaagt
    24181 accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca cggcgaactt
    24241 tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa cggatacgtt
    24301 tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca
    24361 acccttcacg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt tttacttcac
    24421 cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata ctttcgaatt
    24481 tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc gcaagacggc
    24541 tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct tctgcggttt
    24601 caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct ttcttttcta
    24661 attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt
    24721 cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc aagacacgca
    24781 tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc ggtacacctg
    24841 gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt ccgactacgt
    24901 catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa gcttccagtg
    24961 cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct cccaatggtt
    25021 gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca tcgaccatgt
    25081 gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat ggttcacctg
    25141 tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca cgaactgttt
    25201 cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg atacctaact
    25261 gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata cgtgaaggca
    25321 cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat ggcatatctt
    25381 cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc attttatccc
    25441 cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt acgcctggtg
    25501 acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc
    25561 catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca aagatcgcat
    25621 gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact ttccctacaa
    25681 gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg tctaaatctt
    25741 ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt cctaattttg
    25801 tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg tcatctttta
    25861 ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa gtcatgaaac
    25921 caactaaaac gttttggcct agagccattt cgccttgttc catagatggt ccgtctgcca
    25981 gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg ttgtagcttg
    26041 ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca ccgttgtcgc
    26101 ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt ttacataaca
    26161 aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc catggtgctt
    26221 gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag gcacggttgg
    26281 agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg
    26341 atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt tgagcacgag
    26401 ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt gcttgggcta
    26461 cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta acacgacctg
    26521 tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt actttcgcat
    26581 aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg atcggacaca
    26641 tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg tcacgagtca
    26701 atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca agagggtttg
    26761 tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata cttgccacaa
    26821 ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg atcgacattc
    26881 tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt tgtaaaagtt
    26941 caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca ttgccgatac
    27001 cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga cgaaccgttt
    27061 ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca gggtctcttg
    27121 gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg tcttctgatg
    27181 gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt tccatgactt
    27241 ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga tcaactaatg
    27301 tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg tttactttgt
    27361 aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt
    27421 tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa acgtctttca
    27481 aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc
    27541 gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca aatcctaacg
    27601 cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca taagagatgt
    27661 cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca gtagaaccga
    27721 atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt gaacggacta
    27781 actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt tctgtcatta
    27841 atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg cggtttgtca
    27901 aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga gcttctgcta
    27961 ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga ttgccattga
    28021 agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct aagaaccatt
    28081 ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca ctgattcttg
    28141 cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc aagctcttca
    28201 cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa tatttcaaat
    28261 attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat
    28321 agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt atattaaagc
    28381 cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg caacttcata
    28441 tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa
    28501 cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga
    28561 cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc
    28621 aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag
    28681 gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa
    28741 agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa
    28801 aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc
    28861 catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa
    28921 tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg
    28981 cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac
    29041 ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag
    29101 aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag
    29161 acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa
    29221 tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca
    29281 cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca
    29341 atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata
    29401 tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga
    29461 tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga
    29521 cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg
    29581 gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg
    29641 caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc
    29701 tagcaacagt aggaatcgac tacgaaagct tgggcaaaca aacagggatc atggcagcaa
    29761 aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac
    29821 tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag
    29881 ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc
    29941 atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt
    30001 ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt
    30061 aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt tagggggtgc
    30121 ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc
    30181 agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa
    30241 catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa
    30301 agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt
    30361 tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat
    30421 gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa
    30481 tggatgcatt gctctttctg gctccttact tgcgcaaaac aacggctttg ctgatttaaa
    30541 ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt
    30601 acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg
    30661 ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc
    30721 tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc
    30781 tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat
    30841 catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc
    30901 ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg
    30961 ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa
    31021 aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat
    31081 cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa
    31141 cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa
    31201 gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg
    31261 ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa
    31321 ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg
    31381 gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac
    31441 atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc
    31501 gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc
    31561 aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa
    31621 agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt
    31681 tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg
    31741 atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta
    31801 ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc
    31861 cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac
    31921 gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa
    31981 gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc tgaatctacg
    32041 atgaaagatg ccaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct
    32101 gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga
    32161 ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat
    32221 acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct
    32281 tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct
    32341 gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt
    32401 aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc
    32461 tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa
    32521 ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc
    32581 gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa
    32641 gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct
    32701 tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga
    32761 cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc tccttattct
    32821 atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat
    32881 ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc
    32941 aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa
    33001 aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat
    33061 ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat
    33121 caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta
    33181 cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc
    33241 ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca
    33301 gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt
    33361 cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc
    33421 gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg
    33481 gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg
    33541 cccgatccac actctcgcat agcatatcta aataatccat ttattttcct cctttttatt
    33601 tatcctactt actttttcct aattctagga tacctactta tatgttacta cacaccctta
    33661 ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt
    33721 gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat
    33781 aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa
    33841 tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg
    33901 ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta
    33961 aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta
    34021 gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata
    34081 tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat
    34141 tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac
    34201 ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc
    34261 aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt
    34321 ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg
    34381 tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc
    34441 cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg
    34501 taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg
    34561 atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa
    34621 tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt
    34681 ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc
    34741 ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa
    34801 atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca
    34861 atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct
    34921 cagattcatc aacttcaagc gacagtac
//
LOCUS       DARBFD010000019        34938 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000019 DARBFD010000000
VERSION     DARBFD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.019"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(59..187)
                     /locus_tag="SCK08_000908"
     CDS             complement(59..187)
                     /locus_tag="SCK08_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1200819.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SCK08_000909"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SCK08_000909"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1200820.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="SCK08_000910"
     CDS             complement(1674..2729)
                     /locus_tag="SCK08_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1200821.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SCK08_000911"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SCK08_000911"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEG1200822.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SCK08_000912"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SCK08_000912"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEG1200823.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SCK08_000913"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SCK08_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEG1200824.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SCK08_000914"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SCK08_000914"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEG1200825.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SCK08_000915"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SCK08_000915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEG1200826.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="SCK08_000916"
     CDS             10491..12050
                     /locus_tag="SCK08_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEG1200827.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="SCK08_000917"
     CDS             complement(12161..14092)
                     /locus_tag="SCK08_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1200828.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SCK08_000918"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SCK08_000918"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1200829.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="SCK08_000919"
     CDS             complement(15021..15773)
                     /locus_tag="SCK08_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1200830.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="SCK08_000920"
     CDS             complement(15932..16141)
                     /locus_tag="SCK08_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200831.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="SCK08_000921"
     CDS             complement(16152..17480)
                     /locus_tag="SCK08_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200832.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="SCK08_000922"
     CDS             17616..17771
                     /locus_tag="SCK08_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200833.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="SCK08_000923"
     CDS             complement(17845..18432)
                     /locus_tag="SCK08_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEG1200834.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="SCK08_000924"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="SCK08_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEG1200835.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="SCK08_000925"
     CDS             complement(20012..21301)
                     /locus_tag="SCK08_000925"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEG1200836.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="SCK08_000926"
     CDS             complement(21455..22387)
                     /locus_tag="SCK08_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200837.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="SCK08_000927"
     CDS             complement(22544..23203)
                     /locus_tag="SCK08_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1200838.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="SCK08_000928"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="SCK08_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG1200839.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="SCK08_000929"
     CDS             complement(24244..24534)
                     /locus_tag="SCK08_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200840.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="SCK08_000930"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="SCK08_000930"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEG1200841.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="SCK08_000931"
     CDS             complement(26820..28175)
                     /locus_tag="SCK08_000931"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEG1200842.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="SCK08_000932"
     CDS             complement(28199..29368)
                     /locus_tag="SCK08_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200843.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="SCK08_000933"
     CDS             complement(29365..30252)
                     /locus_tag="SCK08_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEG1200844.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="SCK08_000934"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="SCK08_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEG1200845.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="SCK08_000935"
     CDS             34332..34853
                     /locus_tag="SCK08_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1200846.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9427 a         7585 c         6298 g        11628 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
    34921 cggaagcaga agcaactc
//
LOCUS       DARBFD010000020        34636 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000020 DARBFD010000000
VERSION     DARBFD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.020"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            591..1280
                     /locus_tag="SCK08_000936"
     CDS             591..1280
                     /locus_tag="SCK08_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1200847.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="SCK08_000937"
     CDS             1267..2475
                     /locus_tag="SCK08_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1200848.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="SCK08_000938"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="SCK08_000938"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEG1200849.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="SCK08_000939"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="SCK08_000939"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEG1200850.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="SCK08_000940"
     CDS             complement(6578..7273)
                     /locus_tag="SCK08_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEG1200851.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="SCK08_000941"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="SCK08_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEG1200852.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="SCK08_000942"
     CDS             complement(8472..8663)
                     /locus_tag="SCK08_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEG1200853.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="SCK08_000943"
     CDS             complement(8684..9574)
                     /locus_tag="SCK08_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEG1200854.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="SCK08_000944"
     CDS             complement(9561..10328)
                     /locus_tag="SCK08_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1200855.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="SCK08_000945"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="SCK08_000945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEG1200856.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="SCK08_000946"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="SCK08_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEG1200857.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="SCK08_000947"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="SCK08_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEG1200858.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="SCK08_000948"
     CDS             14899..15282
                     /locus_tag="SCK08_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200859.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="SCK08_000949"
     CDS             complement(15450..16208)
                     /locus_tag="SCK08_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEG1200860.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="SCK08_000950"
     CDS             complement(16226..17044)
                     /locus_tag="SCK08_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1200861.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="SCK08_000951"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="SCK08_000951"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEG1200862.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="SCK08_000952"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="SCK08_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEG1200863.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="SCK08_000953"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="SCK08_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEG1200864.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="SCK08_000954"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="SCK08_000954"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEG1200865.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="SCK08_000955"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="SCK08_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEG1200866.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="SCK08_000956"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="SCK08_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEG1200867.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="SCK08_000957"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="SCK08_000957"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEG1200868.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="SCK08_000958"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="SCK08_000958"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEG1200869.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="SCK08_000959"
     CDS             27086..27385
                     /locus_tag="SCK08_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEG1200870.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="SCK08_000960"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="SCK08_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1200871.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="SCK08_000961"
     CDS             27973..28209
                     /locus_tag="SCK08_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEG1200872.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="SCK08_000962"
     CDS             28352..30325
                     /locus_tag="SCK08_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEG1200873.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="SCK08_000963"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="SCK08_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEG1200874.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="SCK08_000964"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="SCK08_000964"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEG1200875.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="SCK08_000965"
     CDS             33204..34496
                     /locus_tag="SCK08_000965"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEG1200876.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DARBFD010000021        33041 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000021 DARBFD010000000
VERSION     DARBFD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.021"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1872
                     /locus_tag="SCK08_000966"
     CDS             <1..1872
                     /locus_tag="SCK08_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1200877.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            complement(3807..4472)
                     /locus_tag="SCK08_000967"
     CDS             complement(3807..4472)
                     /locus_tag="SCK08_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1200878.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            4652..6634
                     /locus_tag="SCK08_000968"
     CDS             4652..6634
                     /locus_tag="SCK08_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEG1200879.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            6638..7276
                     /locus_tag="SCK08_000969"
     CDS             6638..7276
                     /locus_tag="SCK08_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1200880.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            7304..7732
                     /locus_tag="SCK08_000970"
     CDS             7304..7732
                     /locus_tag="SCK08_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200881.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            8189..9100
                     /locus_tag="SCK08_000971"
     CDS             8189..9100
                     /locus_tag="SCK08_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1200882.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            9317..9754
                     /locus_tag="SCK08_000972"
     CDS             9317..9754
                     /locus_tag="SCK08_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200883.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            9849..10733
                     /locus_tag="SCK08_000973"
     CDS             9849..10733
                     /locus_tag="SCK08_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1200884.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            10957..14124
                     /locus_tag="SCK08_000974"
     CDS             10957..14124
                     /locus_tag="SCK08_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200885.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            14144..14689
                     /locus_tag="SCK08_000975"
     CDS             14144..14689
                     /locus_tag="SCK08_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HEG1200886.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            14717..15172
                     /locus_tag="SCK08_000976"
     CDS             14717..15172
                     /locus_tag="SCK08_000976"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HEG1200887.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            15169..15927
                     /locus_tag="SCK08_000977"
     CDS             15169..15927
                     /locus_tag="SCK08_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1200888.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(16013..16753)
                     /locus_tag="SCK08_000978"
     CDS             complement(16013..16753)
                     /locus_tag="SCK08_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1200889.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            16814..18067
                     /locus_tag="SCK08_000979"
     CDS             16814..18067
                     /locus_tag="SCK08_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1200890.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            18389..18616
                     /locus_tag="SCK08_000980"
     CDS             18389..18616
                     /locus_tag="SCK08_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1200891.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            18739..19065
                     /locus_tag="SCK08_000981"
     CDS             18739..19065
                     /locus_tag="SCK08_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1200892.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            19133..19846
                     /locus_tag="SCK08_000982"
     CDS             19133..19846
                     /locus_tag="SCK08_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEG1200893.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            20156..21007
                     /locus_tag="SCK08_000983"
     CDS             20156..21007
                     /locus_tag="SCK08_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1200894.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            21128..22993
                     /locus_tag="SCK08_000984"
     CDS             21128..22993
                     /locus_tag="SCK08_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1200895.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            23041..24450
                     /locus_tag="SCK08_000985"
     CDS             23041..24450
                     /locus_tag="SCK08_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEG1200896.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            24558..25259
                     /locus_tag="SCK08_000986"
     CDS             24558..25259
                     /locus_tag="SCK08_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1200897.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            25418..25897
                     /locus_tag="SCK08_000987"
                     /pseudo
     CDS             25418..25897
                     /locus_tag="SCK08_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            26745..27320
                     /locus_tag="SCK08_000988"
     CDS             26745..27320
                     /locus_tag="SCK08_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEG1200898.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            27320..27760
                     /locus_tag="SCK08_000989"
     CDS             27320..27760
                     /locus_tag="SCK08_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1200899.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            27776..28477
                     /locus_tag="SCK08_000990"
     CDS             27776..28477
                     /locus_tag="SCK08_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1200900.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(28965..29390)
                     /locus_tag="SCK08_000991"
     CDS             complement(28965..29390)
                     /locus_tag="SCK08_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200901.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            29764..30735
                     /locus_tag="SCK08_000992"
     CDS             29764..30735
                     /locus_tag="SCK08_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1200902.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            30767..32164
                     /locus_tag="SCK08_000993"
     CDS             30767..32164
                     /locus_tag="SCK08_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1200903.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            32323..32946
                     /locus_tag="SCK08_000994"
     CDS             32323..32946
                     /locus_tag="SCK08_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200904.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT        11262 a         5347 c         6785 g         9647 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttacccc accccgttgt
     2041 tatatgaccg tgttttgaga ggaaccaata aaaagcatag gctaactagg ggttcctttt
     2101 tcttttgttt ttttggaatc cgcttaagtg aaatgtatct gaaatgaaac ggagataaag
     2161 tgattttttg ctataatgac aaggttttag ctgtttcaga taccatgtaa cccaaaatag
     2221 tttattgaga aaataggaga gtatttgata ctaactagta ggaaaaaatg agataaaaat
     2281 taaatggaat catttcaggt aatctcaaat aaaaaaatat acagtcctgt ggcactttac
     2341 ctatgggact gtatattttt tactctcagc gggcttgttt tgactccgtg tctgactccg
     2401 tcgtattgat tttaaatgat attcgatgac catttaacag gtcatttttg aaatttttgt
     2461 ctctatatga ctcagtttga taattattga tagatcaatt tgggtctata tcgaacaagg
     2521 gaataatcca ataaaaaatg atttagaata tcaaaataac aaaaaagagg actacctgta
     2581 ttcgtcctct tttttgacca gattttgacc agtctaattt acattctaat atattcatat
     2641 gagctaattt tttgattaaa tggtaagttt ttccttgttt gttttagttt aaactagtgt
     2701 gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa ggctaggaac
     2761 ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat aatttctttt
     2821 aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca gagccgaaac
     2881 agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga gttggcaaaa
     2941 gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta actgtaacaa
     3001 gctaatttta agagattctt attagaagac gtttaggaat ataactaaga aaatgattat
     3061 acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt taagaaaaag
     3121 gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat ttcctactaa
     3181 tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat aaaaaaatgc
     3241 ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga aaaaattgtt
     3301 ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt ttatcattat
     3361 gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata aactaccagc
     3421 gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg agcagatcat
     3481 agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact acgtaccaat
     3541 ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa aatatttttg
     3601 tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt ggcgtaaata
     3661 ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga actttcatct
     3721 caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt gcattgattt
     3781 tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg gcttcttttt
     3841 aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat ggcttcccaa
     3901 gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc atggtttttc
     3961 tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa gatatcatta
     4021 catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt atagccgatt
     4081 tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg ataattgata
     4141 agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt tttgagtgag
     4201 aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg caatgttggt
     4261 tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga
     4321 tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca actagaaatc
     4381 gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt acaatttctg
     4441 cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt ctacagtata
     4501 tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg aaaaacggta
     4561 tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac
     4621 agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg ttagtataaa
     4681 taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt atactccgat
     4741 tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca tggtatttga
     4801 aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg aatccaatga
     4861 agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat tgtattatca
     4921 aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct ttcgcgtaac
     4981 gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct tgatgacgag
     5041 agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca atcaagcatt
     5101 tcacactgat tatgccacta atatttttga agactttgaa aatagctatt cgtattcggt
     5161 tatttcaaat acagacgtat ataattatcg agtgtattta actattggat tgattctttt
     5221 attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt tattgttgaa
     5281 taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga aaatcattat
     5341 tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata aggagcaatt
     5401 tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta tctctttgat
     5461 ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact atatccaata
     5521 cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca atgtcgaatt
     5581 agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac caaaagaaat
     5641 cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc acgtggattt
     5701 tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa ctttgaataa
     5761 aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg aacagtttgg
     5821 cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg atgtctctgg
     5881 aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc ctgaacgata
     5941 tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc aagaaatcag
     6001 tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata catttattcc
     6061 ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga aaaacagtat
     6121 gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga tcaagatagg
     6181 ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac gtgataactt
     6241 gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt taagcagatc
     6301 atttcattac ttgatacaga tatttttact cactttcttt tctttcttct tgttgagcta
     6361 tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag
     6421 tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca tcaaatgggg
     6481 aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc tttttaatat
     6541 ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt ctacagaaaa
     6601 gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga tagtgataaa
     6661 aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt tcactgcgga
     6721 tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta cgttattgaa
     6781 tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg atcaagagac
     6841 gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat cttttctttt
     6901 tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata ttggattgaa
     6961 gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat tgaaaaaagt
     7021 aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg agaagcaaag
     7081 aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg atgaaccgac
     7141 tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc aacaagcttc
     7201 ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa taggaaacaa
     7261 gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat
     7321 tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg aagtggaagc
     7381 aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga ttcattttga
     7441 tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt ctagaacagc
     7501 aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta cgagaaaaac
     7561 aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg
     7621 aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg
     7681 aaatagaact gacacattct catgttggta caatccaacc ggttggtatt aagtatccaa
     7741 aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta tttttggtag
     7801 ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca acaaaaaaat
     7861 gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa
     7921 acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac gcaagataga
     7981 cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac ttgacattta
     8041 ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac gtatcgagca
     8101 gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt agcaattcgc
     8161 aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac ttgccatcgt
     8221 actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag ccgttagtta
     8281 taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc
     8341 agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat atggagatga
     8401 cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat tgggtgattc
     8461 atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag acagtatcaa
     8521 acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg atgaagattt
     8581 aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc aagtagcaag
     8641 tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt tctctaaatt
     8701 agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag tgaaatttga
     8761 ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt tgaaagatgg
     8821 tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg aatattatgt
     8881 ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca aaaaagaatt
     8941 gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata aagtaattgg
     9001 cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa atgttttgag
     9061 tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa taaaaaatat
     9121 gaactattta aatcaataaa actgttcatc taaatctttt tattagaact aaatcaacag
     9181 tagtttaatt actacttgat ttagttcttt ttattttaaa aatttttttt acttagaatc
     9241 cagcgaccct agatattttt ctgtataatc ttaaataaag aaaacaatat caaaaaatgc
     9301 acgtagaagt agataaatgg agtggttttg caaatttctt ttatttctag aagttattca
     9361 cgtatacgga tttgtttttc tcacaaataa aaaggcagga agtacaatgg atcatttgta
     9421 tataaaatat attcatcaat tttccgtttt ggtgttaggc acactaatta tagcggggat
     9481 tgtcgcattt attattcaag gccgtgacat attaacagta catcaagagt ttttcagtta
     9541 tgcatttggt attcctctga tcgctataag gtctgataac aaaaagtgga agaaacgcta
     9601 ttacattctc ttgctatttt tgtgttttta tctgccacct aagattttta ctttattagg
     9661 tatacaatgc ctatcatttt cttttatttc ctatatcatt gttttaattt tcctcagttt
     9721 catagtatgg atgttttatc ttgtcaaaaa ataaatgaat gtttcatgaa cagtacttca
     9781 tcacagccta atttttttct ttatgctatc gttatattaa agaaatcaat cagaagggaa
     9841 agaaagtgat gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt gaacaagcaa
     9901 aggaatttga gcaattagcg atagcaaaat tggaatcggt gtttgatgaa gtggtcgtct
     9961 tgcatacaaa aaaagcaggg gatgcaaaaa actttactcg cgaagcggct acggaagggt
    10021 atcatagtgt gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc agtggaatcg
    10081 ctgaacaaga acatcggcca aatttcggat ttttcccatt gggtacggtg aatgacttgg
    10141 ctcgagcatt agggataccg ctagaacctg aggaagcgat caatcatttt tcgattgaat
    10201 cagtcaagcc attggatatt ggaaaaataa atgacgacta ttttatgaat gtcgtcgcaa
    10261 ttggctctat ccctgaatca atcaatgatg tagatgcgga gaagaagacg aaatttggaa
    10321 aactagctta tttcatgtca ggaatcaaac agttagcttc cacacaatcc tactcgtttc
    10381 acgtagaagt tgatggaaag aaggaagaaa ttgagagcag taccttgctg ataggactaa
    10441 ctaactcagt cggcggtttt gaaacattac ttccaaatgc aaaagtggat gatggtaagc
    10501 tccatttggt ttatttaaaa gattcttctt tgatcgatac gcttaaaaca ttgccagacc
    10561 tattaaaagg agtagatgaa tctacgaaaa atctagccta ccttacttgt gagcaattag
    10621 ttgtgtcatt agctgatgag gaagaattga cgacgaatgt tgatggagat gaaggggata
    10681 aattacctgt gacaatcaaa gtattgccct cacatttaca ggtttattgt taaaatggag
    10741 gttgtgaccg aggagttggc gggagaacac cgcttgttcg ggataaggga ctgtgacaag
    10801 acttttgtca cagccttttt tttgtacaaa aaagaaattt acacctaata gaaaaatttt
    10861 tccataaaaa tacatatttt aaatatgaaa gtacaattgg aaatagatta aaacgataaa
    10921 ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatgtgt aaaaaagctg
    10981 attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt tgccgatgta
    11041 gagaaaacaa acgaatcaac gcaatcagag aatcaaagtt ttgacaagac tactttttca
    11101 gaagaatcga ttacttctga aactacagaa tctcaaagtg aggcttctac taataaaagg
    11161 caagaagaaa ataagacaag tgagacctca gaagtagctg aacaaacgtc tgatagcagt
    11221 gaagcggcac tggcaagagc tgtttcagcg gatgggtaca gcgaagctgc cacaacagaa
    11281 gaactggcgc agcttctcgc agacgaatca gtaactaaga tccgactgat tcagcctttg
    11341 actttagaca gggaattaga gatcaaacga gatatcgtta ttgattttgg tggattcgct
    11401 cacaatttcg gcacacacca tatttatatc aatgagatac ctcagtcgat cgagttccag
    11461 aattttaaag gaacagctgc acatccaggt ggattgacac caaatatcga tggaaatgcc
    11521 attatcattg cttatatgac cgatttttgg ggaagcagat atcattttac gggagaaata
    11581 aaatttaccg ggacacttga tttgtatgat gggtccaaat taggactgat ttacgctcct
    11641 cgtgcaactg tcacattaga tggtgtttcc ggtgtgttgg atgtccaacc agtgaaggaa
    11701 ggactgaatg ccgctccggg taaagcctat ttttgtagaa gctatcaatt gaatgtgatc
    11761 aatggtagtc aactttatgg accgtatcta gggaaattct acggttttct agattcagga
    11821 gatgagactg gtagtggaaa aacagcgcca ggaatccata tcttaagcgg ttcaaaggtc
    11881 tctcttgatt atgatcgggc agatggcgtt agcgatgaag gagaggcagt cgatacgcta
    11941 cctggtaatg tcatgtttga agtcagtggt tcgggaagcg aattttcggt aaatacaaag
    12001 atcaatatta ccaatgacaa caatcgcgga atcatccaaa tgagaggttc aggatcgaaa
    12061 gtcactgtat cgaatggtgg gaaaatcacc atcaatacag caactacggg cggatttcgc
    12121 ttgcaaggag attctagtca aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg
    12181 gctggagatg acgatcaacc cgggaacaat gggatgcgtt ttgtaggaaa aggcctttct
    12241 ttgatcgtag aaggagccgg atcagttatc gacatcgata agcagagtgg gcgttccagc
    12301 gcagttcgtt ttgagaatgg tacgcaaaag ctgacagtaa ccaatggagg ctcattgagg
    12361 gtaagaaata caggtgatgg caaatcttac gtgaacagac tcaatcaagg aaatcaagcg
    12421 atccaatttt atgacgcttc tggttttcta gaaagtccag gttcagctga ttttacagtc
    12481 actggagaaa aatcaaatat cgatatacgc gcagattccg gagcaactgt agatacaact
    12541 ggagatattg atttaaattt tacggcaggt gaaaaaactt atttagtcat ggtcgggaaa
    12601 acaggggaag ataagaccgg catcatcagt ggggatgtgt tgaatgtgga cttagaatct
    12661 cctacctatt ttgatttcca aaacaaacgg attggaagcg agaccaatgg cataggcggc
    12721 tgggtattcc aaggaaatgg agattcttct ttaaagatcg cccattcaga aatcgcttta
    12781 tggcgaaaaa caggcgcaac attcgataac tttgaaaaag acccggatta tttttctgat
    12841 atgacagact tatcgatcaa tgggaccgac ttaggcaatt tcgtatcaag ccctagtgca
    12901 ggtttaacaa atgaatttgc tcagacaggt gccggaatga aaaattataa tcgaatcagt
    12961 gccaataatc agctcccgat gattgaatcc ttaagagtgc caacgaatgc agataaaaaa
    13021 atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca
    13081 ggcgaagtag aagtgacttt acgtattact tacgcagata cgacgaaatc atctgtagaa
    13141 attactgctc tcacgcaagg acaaacagat gaaaatgaag gggcaggata taacccatgg
    13201 ggagaaggcg aacaaggcgg cttgttcgaa gttcaagtac ccaatgatga gagattaaaa
    13261 acggaggatc aagtgcaagt tgttgctgct aaaaaagtaa gaggtggcca agaagtgaca
    13321 gaactggact ctgccaaaac gacagtcgat gtgattcctc ctgaacctgc taatttaagt
    13381 accacattga tcaatgcagc ttctcgcacg atcacaggta caggtgaagc aggagccttg
    13441 gtttcattga aaaaaaatca agctgttttg tcagaaacaa ctattgatga tacaggggaa
    13501 tttgttcttt ctatcccaga aaatcaactt tttgcaggag atgtattaga aattgtatta
    13561 aacgaccaag caggagaagc tactgctaag ggtgtgcaga acaagccaag taccaacgat
    13621 gaaattggga atcacaatcc aagtgatacg ccagttgttt atcatgatgc cccgcctttt
    13681 gcacctgcta cgaagatcgt agtagaaggt ggtctctcat ttcttgcccc ttccaaactc
    13741 gattttggtc agatcaaagc aactggtttg acacaagaag catttggcca aatggatata
    13801 caggaaaaat tatggattta tgatcagcga gaaacgaaaa atccgtggat cttaacgata
    13861 aaattatctc agcctttcac aaatgatgca ggattttcta tgcaggatgt cctatatttc
    13921 aagaaaggag aggagtatca attagtgaat gaagaagaac tggaaattgt cagaggacaa
    13981 aattcaacga atgtgcaagc agattattca gaatatttaa ataacggtcg ggcgtttaaa
    14041 cttgtcttaa ccaaagaaaa tcagatagct ggtaattact cagcagagat aacatggaca
    14101 ttagcagatg cgccgacaga ataaagatca aggaggatga ttgatgaatc gaatatggaa
    14161 taaaagtcat cgaaacaact gctctttact tatgatcctt ctccttggat tcatcctttg
    14221 gcagacacct gtccatgcct ccgaattcaa ttttgcggta gattttgaat taccggctaa
    14281 tcagattgat aagaacaaaa catatataaa tgtaaggatg gagcctggga aaacacagga
    14341 aatcccctac acggtcttta accgaacaga taaatcagta acagttgaga cattgatcca
    14401 ctccgctacg acgaattcaa acggtgtgat cgaatatgga gaatcaaaaa gtaaatttga
    14461 tgaaacatta agttatgata tgaaaaactt agcaaaaaca gtgggagaaa taactgttcc
    14521 tcctcaaagt gcggtcactg agacactttc actgaaagcg ccagaaaaag agctggctgg
    14581 tgtggtcgca ggagggatca cgtttcaaga aaaaaaagag aacgtttcta acaaaagtac
    14641 agttggtgtc gtcaatcagt tttctcaagt gaaagcaatc ttgatttgaa atagcgaaaa
    14701 agagtcttcc ccagatttga aattaaaaaa agcttatgcg gatcaaagcg atggaagaaa
    14761 tgtgattaag gcgaatcttc aaaatccaaa tgcttcttat atgttcggct tgactacatc
    14821 ggttgaaatc tacaaaaaag aaacaaaaga gccttacctt tcaataaaaa aagaaaaaat
    14881 agatgtagca cccaatacaa attttgattt acttgtccca ttagaaggaa aaaaattagc
    14941 tccgggacat tatcaagcgg ttgtgaaggg tgcttggaaa gaaaaacgct ggaatctcca
    15001 taccgatttt gaaataactg atgatgcagc ggaccaattg aatgaaaagg atattgattt
    15061 aaaaaatcag ccagaaacat ttgactggcg atggctgata cttgttggag tcattcttat
    15121 tggcttattt gctggattat ttattttaaa agaaagaagg aaaaagaaat gaaaaaaaca
    15181 attgcattaa gtaccgttct agctggaagt ctattattat gtacacaaag tgtttttgct
    15241 gagaataaga gctcagccac tacagaagga acgatttctg tgaccaaacc agatgctgca
    15301 aatcctgaag acgttgttcc agttcctcct gttgatccaa atgatcctga tactcctttt
    15361 acacctgacg aaccaaatca gggaacatcc ggattactga cgattgacca agctccagat
    15421 cttgattttg gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc attacaaagc
    15481 ggaacggaca gtactggagc acaaacacaa gtacagaatt tcgtacaagt aacggataaa
    15541 agcggaaatt atgccggttg gaaattatca gtcaaacgaa cagaatttat tggtacaaca
    15601 tctgatacga ataaactaac tggttcaaag gtgaaattca aaaacgccgt gattcgaaat
    15661 agcgcaacta atagcagtgg aatgccatct accgtttctg gaactggcgc tgaaggaatc
    15721 gaaatcccag cagatcaggc agtagatcta gtaactgcag gagcaaatga agggattggc
    15781 acatggattt attcattagg agagaatgtg gaacaaggga gacaatcagt ggaattattt
    15841 gttcctgtat ccaattatgc tgtagacaac tacgtttcaa cattcacatg gtcgttaaca
    15901 gatgctccta cagaagagga agcgtaaaat catatcaacg atttcaggga gtggagccga
    15961 ttcaaggccc actccctgaa atctgctatg cttacgtctc agctcttatt catcaactcg
    16021 aagcagtttt ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc tttcgaccaa
    16081 cgagtaagga gtcaccgttg aaaaattcta acacataatg agcagaatca attttctgca
    16141 attgcgataa gttgatacag taagatttta aaagtaaaaa gtaagcatac ttttctaact
    16201 ctgtcttcaa ttgttttaaa gaagtattca ctaatttatc ttctgtagtt gttttgacca
    16261 taacttggtt tttcaaacct ttgatacttt caatatataa aatttcccga ggatacagga
    16321 cgagacttcc acctttttga tcaataaaga ttttatcgac atttacgatc tgctgttctt
    16381 tccaattgat ctgttctagc acttgacgaa tttgttcaga taaattcctg tcttttctga
    16441 tatatccaat cggaaggatt ttccgattga tgatctctac tgcaaaattc gtataagcgg
    16501 tgaaaaaaac aatattacta tgtgcatttt ttttacgtat ttgttcggcg acatcaatac
    16561 ctgtgagtga ttgcttcaag tcgatatcga taaagaaaac atcgtgatta tcaatagtgc
    16621 ccaactggat caactgattg atttgagaga tagaagtgag ctgcacgata ttctcgatat
    16681 gatgattgac caattctctg atgatccatt cgctgtgaaa ctggttatct tctatgataa
    16741 aatacttttt catatgcagg ctcctattaa aaaaatatgt gtacattttg aaacaaaaca
    16801 atggaggaat aagatggtcg caacagtgac actttatttg acctttactt tgattttaga
    16861 tttaggtatc tatttattct tttcgaaaca actgctagta aaaaacagct ttggttgtat
    16921 agctttgatg gtggtgacac ttttacttcc tatccctaaa gtgagctttc cttacctagt
    16981 tggcttatta ttgatgtttc ttttttattt tactacgaaa aacctgttta tcagctgttt
    17041 gatcagtaat cttttttttg ctatcactgg actcagcttt tttctggttt ttgatctgat
    17101 ggattattta ggaaagagac atcttggaac agcaagtttt tttgcactac ttatcgcatt
    17161 tattttctat ctattagtaa aaagtataga caaacagacg agatttctca acttgctttt
    17221 tacttatcca actgaacact attttggagg tatttgtatt ttattaagct ggggcgtgat
    17281 ttacttggta ttgggaagtg ttcacttccg ctcatctaat tatctgcttc tatctctttt
    17341 gtcgatagtc atcagttttt tttctttgtt tttttccatt atgttgatca gtttccaagt
    17401 aaaagaaaga caacatcagg aatcgcttca actgtatcaa ataaatgaag aatattacca
    17461 tcatctagag gaattcaaac atgattataa aagtctactg ttttcattaa aaattactct
    17521 tcaggaaaat aaaaaagaaa cgttggaatt tttagaaata ttagaagatc attctgcagc
    17581 tatttttgaa aaaccgcaaa tacaacagct aacaaatatt gtatccccag ctgtcagggg
    17641 tgtcttttta aaatttctag aaaaagcaga atcggagaaa atcccgatcc aagtagttat
    17701 tcctgtcgaa gtgaaaacaa tcccgattga tctggttgtt tttattcgct gtttatctat
    17761 ttttatttct aacgcattcg atcatcgtgt agccgatcag agtccgatta cgatcaatct
    17821 gctggaagaa aaagagggat cacgttttag tatagcgaac aaggcaaatc caacgaattt
    17881 gacgctatcg gagatggtca aaagaggaaa aacctctaaa aaaaatggcg ggttaggtct
    17941 atacagtgcg aaaaaaatgt tggatgttta cgaaaatgca gaactaaaaa tcgaattttc
    18001 aaaacagaat cactattttt ttgtagaaat gtatctggca tctagaaaga gcaaatcagc
    18061 ctactgactt actgtcaaag gataagtttg aggaaagtct acacggagat agagacaaca
    18121 acacagcata ttcaaaagaa acagaagcgt gatgaccgtt gtcacccttc tgtttctttt
    18181 gctatgtagt taaacaaagt agttgacact tccgctactt agtattagta cgaaagtata
    18241 cttcatgata ctaagtagat aagaagtgaa aagcggaacc cacgatcgtc aaaatgctaa
    18301 ctgctttgct tacaccagat aaagggaaga gcaatatcgc ttattttagg cgtggagaag
    18361 caagcaaatc cctacgatca caaaaaaaat gctgacccag cagttaagag agttagaaca
    18421 agatgaaagt atccatcgaa aagcctataa ccaagtcccg cctaaagtag agtactcgct
    18481 caccgaagaa ggaaaatcat taagagagat tctagtagcg ttgtctgttt gggaagaaga
    18541 acgtatcgaa aaacaaaaag cctcaaggga agaagacacg ctactgcata cacatggtgg
    18601 ctacctaaat tattgatttt ctatgacatt tattttaaat aaaagaaaac aggagttttt
    18661 ttaaaagcat catctatttt ttatgtaaaa aaatggtatg attaatcaag taagaagtga
    18721 ttggaagaag gcaagcaaat gaatagtatg gaaaaagtca accagtttag agatgaacgt
    18781 aattggcgac aatttcataa cgaaaaagat ttggctatct ctatttcttt ggaagcatct
    18841 gaattgttag aactttttca atggaaaacg ccggaagaag taaaagaaac acaattagaa
    18901 cgaatcaaag aagaactggc agatgtattg atctattcat acatgatggc agacaacctt
    18961 cacctggatc tagatgagat cattgaagaa aaattgatca aaaataattt gaaatatcct
    19021 gtagaaaaaa gcgcaggtgt acgaaaaaaa tacacagagt tgtagaaatg tgtcattcgg
    19081 ctgttggaaa gagcaaaaga aaaacagtaa aatgagtctg gagggagaga aaatgaaaaa
    19141 aaaaggaaaa gcaaaaagat ggctagtcaa tatcctcttg ttcctcttgt tattagtagg
    19201 actagctttg attttcaatg aacagatcaa ggattatttt gtcagagaga caggagataa
    19261 atatgctata gcgaatgtaa caaaagaaga tctgaaaaag aataacgata aagatgtcag
    19321 ttttgacttt gatgcggtag aaccaatgac gacagaaggg gtcatgaggt cgcaaatgag
    19381 aggaacagat ctaccagtga ttgctagtat cgcagtccct tcagtttcaa tcaatttacc
    19441 ggttttcaaa ggcttggata atacgtcact actttacgga gcaggaacat tatctccaga
    19501 tcaagaaatg ggaaaaggaa attatgcctt agcaagccat cgtgcaacga accctgaatt
    19561 gctgtttaca ccgctagaaa atttagagat gggtgccaag atctatttga cagatttaga
    19621 aaatgtgtat acatacaaaa cattttttaa agaaaaagtt gctcctacag atacccaatt
    19681 actgaatgaa gtcgagggaa aagaaattgt cacactaatt acttgtggcg acatggacgc
    19741 cgtcacgcga ctagttgtac aaggagaact ggaaagtgtt acatcgataa aagatgcgac
    19801 agatgatatg agatctgctt tcaatctaga gacaaaaaca ttttaggata ctgggacata
    19861 agtcgttaca gattaaaaac agccaaagga tatgaatcga taaggaaaaa ccaatttgct
    19921 gaaaaaaaga gaaacgcaaa gaggtgcatc tctgatttgg ttgttttcta gatagacttg
    19981 tgttccagtt tttttatttt ttatatgaaa aggcttgcat aaataaaaat tcgatggtat
    20041 gatgattttg aataagaaat ggattgttac tgtacggcag gcaaaaccag aattcagcta
    20101 agaaacagta ggcgaattct aggttttttt atttttcttg agagggcgaa gcaaaatgaa
    20161 aattgataaa attttgaata acaatgtcgt catttcgaaa aatggattcg gggaagaagt
    20221 cgtttgtatg ggaaaaggac tagcttttca aaaaaagatt ggggacgaga tttcgccaga
    20281 agcggtccaa aaagaatttg ttttaagaga ttcttttgct aaaaaccaat tccagcagct
    20341 gatggctgat attccggcag aggagatcga atgggtcaaa caagtagtcg aactggctga
    20401 agataaacta aaagttgaat tttcgcctaa tatttatctg acattgaccg atcatcttca
    20461 ttatgcgatc acacgagcaa aagaaaatat ccaactgcct aatccattat tatttgaaac
    20521 aaaaaaattt tatccgaaag aatatcaagc agcgaaagag gctcttttgt tcgtgaaaca
    20581 aaagacgggt gtggaacttt ctgaggatga agccggtttt attgcctttc attttgtgaa
    20641 tagccaacaa ggaaacgaag acatgcaagt gacgatgacc gctaccacaa tggtaagaga
    20701 tatactgagt atcatcagta aattcttcgg catcgtgttc gatgaagaat ctttgaatta
    20761 tcagcgtatc attactcatc tgcagttttt tgcccagcgt tatctcaaag gggaaggatc
    20821 tgacgagcaa gatgaatttc tctacgcatt agttcaagga aagtatccaa aagcgtttca
    20881 ttgtgtcgaa cggatcaatg agtatcttcg acaaacgcaa aaacaggaaa tggggatcgc
    20941 agagcagatt tatttaacga ttcatattca aagagtagta agtgagaaaa aaagcatcca
    21001 atcataagga ttgttactgt atggcaggca aaacctggat tgaaaaaata ggttcacgaa
    21061 tgtttgtgac tgtttttttc aatctgggtt tttatttttt aaataaaaaa gaaagaagga
    21121 aaaaacaatg gataatcaag cagttggtcg tcgtgtatgg gaagcggtag gaggacaaaa
    21181 gaatgtcaaa agtttagtac attgtgctac gcggttgcga ttcagattaa aagatgaatc
    21241 tttagcagat acgcaaaagt taaaagaaga cccggacgtg atccaagtcg tacaaagtgg
    21301 cggtcagtat caagtagtga ttggcagtaa tgtagcagat gtttatcaat cgatcgtgga
    21361 tgaggaaggt cttgcagttt cggaggatgg aaaagaagag tccaaaaata ttttgaatcg
    21421 tctgatcgat attatctcta gtatatttac accttttcta ggggcgatgg cagccgccgg
    21481 ggtattgaaa ggattcttat cattggcaac ggtgatgggc tggctgacag ctgatagcgg
    21541 tacttatcaa atattgtttg ctgcagcaga tggtgtcttt accttcttac ctgtcatgct
    21601 tgcttttaca gcagctaaaa agttcaaagc caaccagttt ttagcagtag caattgctat
    21661 ggcacttgtg tatccagcta tcacagcagt tgctggtgcg ggagaagcta tcagtttctt
    21721 tgggatccca gtgattcttt caccatctgg ttatacttct tctgtcatac caatcattct
    21781 agccgtatgg gttcaaagta aattagagcc attcgtaaaa aaagtgattc cgcaatttct
    21841 gcaaatgatt ttagttccgc ttgtcgtttt agtagtgatg gttccattaa catttcttgc
    21901 tttaggacca atcgggacgg ttgccggtaa tgctttaggc ggtctcttca actcgattta
    21961 tggatttagt ccaatcgtag caggtctaat catgggaagt ttatggcaag tattcgtgat
    22021 gttcggcatg cattggggat tcgtaccgat catgtttttg aatatcgaac aatatgggtt
    22081 tgatgtattg atgccaatgc ttcttccagc catcttggca caaggtgggg ctgctttagc
    22141 tgtagcattg cgaacaaaag atacgaaact tcgagcatta gggatttctt ctacagtgac
    22201 ttcgttattt ggaatcactg aaccaactgt gtatggtgtg acattacctt tgaagaaacc
    22261 attcattgcc gcttgtattt ctggaggtat tggtggagct attatcggat tttccggagt
    22321 aaaagctttc tccagcagtt tggtcagctt attgacgatt ccgactttca ttaatacggt
    22381 ggatggtgta gagtcaaatg tgacagtagc agtaatagct acaggaattg cttttgttct
    22441 tgcattcgta ggtacattga ttttaggatt tgacgaacaa acacaagaca atcaattaga
    22501 aaacaaacat gcgaatgcag gaaaaccgat tacttcagca cggcatacct taaaaagtcc
    22561 tttaacgggt aaagtactgc cattgagtga agtaccagac caagtttttt cttctggcgt
    22621 gatgggaaaa ggtatcgcta ttgatccaga agtaggagaa ctagttgcac ctgcagacgg
    22681 agagatcacg actatttttc caactggaca tgctgtaggg atcacaacga ctgacggagc
    22741 ggagattttg attcatatcg ggatggatac agtagaatta aatgggaatg gctttgagat
    22801 cttagtcaaa caaggggatc tcgtcaaagc aggagattta ttgattcgtt ttgacatcga
    22861 agcaattaga gcagctggct atagcgtaat cactccagtt gtcattacga atactgatgc
    22921 atttgccgat atacttgaat tagaccaaaa agagattatt gcaaacgaag atgttctggc
    22981 aattgtaaaa taaggaggaa atcaggatgc aatcacgttt ttcagaaaaa tttttatggg
    23041 gtggagcaac tgctgccaaa tcaattagaa ggggcttatg atcgagacgg aaaaggacta
    23101 tccgttgcag atgctatgcc tggaggtaag cagcgctttg cgattatcgg cagtgaagaa
    23161 tttgactgga cgattgatca agaaaaatat atttacccga atcatcgagg aatcgatcat
    23221 tacgatcgtt tcaaagaaga tcttgccttg tttgcggaaa tgggattcaa atgttatcgt
    23281 ttttcgattg cctggacacg aatttttcca aatggagacg aaggtactcc taacgaagcg
    23341 ggattagaat tttatgatca gctgattgat gagtgcttaa aatatgatat tgaacctgtg
    23401 atcacgattt ctcattacga aatgcctctt catttggcaa aagaatacgg tggctggaaa
    23461 aatcgaaaac tgatcgactt ctatgaacga tttgctcaga cagtattaga gagatacagc
    23521 agtaaagtca aatattggat gactttcaat gaaatcaatt cggcctttca ctttccagct
    23581 ttaagtcaag gattagtaaa aagcaatgga gcaggagagt accaaaatat tttccaagca
    23641 tggcataatc aattcgttgc aagtagcaaa gccgtaaaaa tcggccacga attgcgttcg
    23701 gatatccaaa tcggctgcat gattatctat gcaacaactt acagcatcga tgccaatcca
    23761 gtcaatcagg cagcaacaat gatccaaaac caagaattca attttttctg cacggatgtc
    23821 caagtacgtg gagaataccc tgcatacaca gcgcgtaccc ataaaaaata tggagtcgat
    23881 ccggaaaaat tagagcaaac ggaaaaagac ttccagttgc taaaagaata tccagtggac
    23941 tacatcggat ttagttatta tatgtctacg gcaatcaatg agactgatcc acaagcagca
    24001 acttcagaag ggaacttgct gggaggagta aaaaatccgt ttcttgaggc aagtgaatgg
    24061 ggctggcaga tcgatccaga agggctgaga atcgcattga atgaattata caatcgttac
    24121 cagaaaccat tattcattgt tgaaaacggt cttggagcca ttgatcatat ggaagaggat
    24181 ggcacgatta ttgatgatta tcggatcgat tatttacgtc gtcatatcga agcgatggct
    24241 aatgcagtag cagacggtgt agatttgatg ggctatacgc cttggggatg tatcgatctt
    24301 gtcagtgcat caacaggaga aatgagtaaa cgttacggct ttatctatgt ggatctagac
    24361 gatgaaggaa atgggacact gaatcgatca aagaaaaaat cttttgattg gtataaagaa
    24421 gtaattgcct caaatggcgt aaacttgtaa ttaaagcagc tgaatgaaaa agaacacact
    24481 tctcacatgt gatttgtgtt ctttttttat tttttatgtg ctattctttt agtaatagta
    24541 aagcaaaggg tgacgttatg aaaaggacac gtgtactata cttggaagtc gcagataaaa
    24601 ttaaagaaga tattttctca ggaaaatatc cagtcggttc gatgttgccg acagaaagtg
    24661 aattagaaga actgtttcaa gtcagcaaaa tcactgtccg aaaggcaatc gaactgctgg
    24721 catcagatga atatgtcgca aagaaaagtg gtcgtgggac gacggttcta agcaatcgtc
    24781 cttacaatcg attatccaaa gcggcaagct ttacgcaaat cttggagaac tcccgatatg
    24841 aagtccggaa agaaacgctg gatttgcaat tggtccatat aaagaaggaa gatcctttgt
    24901 tttccttttt cggtgagcaa gctgtttgtt ttaagcgctt atattatttg aacaatcagc
    24961 cttatattta ttttgttcat tatcttccac aagaaatgat gcaattcagc agagaagaat
    25021 ttggcaaaga atctttgtat cgtttgctga cgaaaaaagg atatactatt gactcatttt
    25081 ctgatgattt ctcggcagta tttttaacag aagaagaaca aggaattctg aaaacagacg
    25141 cacagatcgg catcaaacgg atacggaaat cgtctacgca aattggggaa atgattgaat
    25201 attcggaggc ggtctaccat acggagctgc atccttacca tattgattat gaaacataaa
    25261 taaaaaaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa
    25321 atagcttctc atatttttcg tttggttagg cttattaccg cagatacacg gctgtttctg
    25381 gattaccatt tattctggat aagggggtgt ggcacgattt tcagaaggaa tctatgaacc
    25441 aatcactgta ttgattttag cttcgatggt aaccccattc ggcttgacga tcgcttattt
    25501 cttagggaaa atcattcgta aaaatatact caatcgtcaa gaaatcgata cattgaagac
    25561 cgcttttcct atggggatct gtcagatcac agagggatgt ttccctatcg tattgaacga
    25621 cttggcacgc aatgtcatag caacgggagt cggtgggaca gtaggtggcg gattaagcat
    25681 gttttggggc gcagatagcc gtattccggc atcgggaatg tttgcagtcg ctacgatgac
    25741 cagaccatgg gcattcattg gtgcattgtt agctggttct tttgtcacag gaataaccat
    25801 tttattattg aaaaaaccag tagatccgaa tgcagaaatc atccaagaag aaaaagagga
    25861 agaagacata tcttgggatg atttgacaat tagttaggag aggcagaatg gattacaaga
    25921 aaaaagtaca atagatgtac cttgagacag gaatggtatg aaagaatcag agttagaaag
    25981 aatcgaatat acagattttg gtttaggagc gatcgaaact gtccagagca tcggcaccct
    26041 ccttgaaatg acgatgaagg aaaaatgaga acctttcgtt gtcgaaaggg tctggtatac
    26101 cttatgtcga aggaaaggaa acaaaaaaac tttcctgtac tattccaaaa aggaaagaaa
    26161 gttattatac agtaaaaaaa gaaattattc ttcatgctag agatcagtac acgttcgagc
    26221 ccaatatcaa acactagttc caggcaggcg caacaggtgc ggttatcact gaattttctt
    26281 ccagcagtga cgattcaagt gatattttta caaatccagc tatttatcgt taacaaaaag
    26341 atttttagga gaagctgatg aacaaaaatg atcaaatttt gatcgtttag ttcgtcggct
    26401 tttttattta gagaaacatc atttatcagt gaaacataac acgagaacaa aatgattttt
    26461 tcgttaataa aagaaacaag tatttgttgt ttaaaataat acacgttatt ggcatatagg
    26521 acgaaatccc agtatcttct ataagtaagt catttcatat cgatgaaaac acgttattta
    26581 tttttgtttt atctataaaa tggctgaaaa atgatttttt tctaaatact atatatagtg
    26641 gcgtctatcc tatataccac taaaaaacta caaaatctag tcttttattt taaaaaaaca
    26701 cgtgtagaat gaagatagac ttttgagtta gggagtgttt ttttatgaaa cttactgttt
    26761 tttgcggttc ccgttttggt aataaagaaa gttacaaatt tattgcgcaa acgttaggaa
    26821 aatacatggc acaagaagaa atcgaactgg tttacggtgg ttcatactcc gggattatgg
    26881 gagtgattag taaaacagta ctggaaaata acggaaaagt cacaggtatc tatccgaatg
    26941 ggctttttga aactgaattg cctggaaaag atgttactac atttattcct acagaaacga
    27001 tcgatgaaag aaaggtatta ttatttgaaa agggagatgc tgtcctcgtt ttccctggag
    27061 gtctaggtac attggaggaa ttctcacaac ttctttcttg gatagcaatc ggtctgacgc
    27121 ccgacaagcc aatcggaata ttgaatattg gcggttatta cagcggcttg caacaattat
    27181 tagaaacttt tgcaaaagag gattttatgg ataaaaaatg gttggatcaa gtaatctttt
    27241 ccaacaatcc attggaactg gtcaatgaat tacgagcagt atctattaaa aatcagttat
    27301 tattgaagga ggcaaaataa tgagtcaaat atatttagca ggaccatttt tttcagagga
    27361 gcaaatcgat cgagtaagtc gtatcgaaaa agcgttggag gaaaacaaaa cagtcacaag
    27421 tttctactct ccacgtcatc accaagaaag caattatgaa ctattcagcg ctggatgggc
    27481 gcaggaagta tacgaaaaag atatggaaga actgacaaat gcagaatttg ttgttgctat
    27541 ccttgacttt gagcatcaaa caatcgatcc aggaacggct tacgaattag gtgtggcaac
    27601 tatgttgaaa aaaccgatga tcattgtaca agaagagacg gtacctacga acttgatgat
    27661 tacacaaagt ctccatacgt atttaaaatc agaccaagct gtacgtgaat atgattttga
    27721 aacattgcct gttgaaactt acgtaggcga gtacttatag aagaggaagg gttgtatgtc
    27781 agtgattttg atttcaggaa cgattggtgc aggtaagagc agtttgacgg atatgcttgc
    27841 aaaagagatt gattctaaac cattttatga aaatgtcgaa gataacgaag tattgccgct
    27901 cttttattca aatcctgagc agtacgcttt tttattgcaa atcttctttt tgaacaagcg
    27961 atttttagca atgaaaaatg ctttagtgaa cgatgacaat gtgctagacc gatccatcta
    28021 tgaagatagc ctgttgttcc atctaaacgc tgatttaggt cgggtaacgg atatcgaagt
    28081 acagcagtac gatagcttat tagatacgat gctaaacgag ttagatgatg ttgctccaaa
    28141 aaaacggcct gatttgatgg tccatatcaa agtttctttg gacacgatgc tagaacgtat
    28201 caaaaaacgt gggcgtgact atgaacagct agaaagcgat gagacgttat atacctatta
    28261 tgaaacgttg aacacccgct ataatcaatg gtatgaagat tttgatgtat gtcctaaaat
    28321 acaagtggat ggggataaat tcgattttgt agaaaatcca gaagatgcaa aaaaggtgat
    28381 ccgtcagatc caagaaaaat tagctgaatt ggaaggaaag cctgctggaa aatattttac
    28441 ggataaccag catgcaaaag gtcaagaatt aggctaaaaa taaaaaacca aacgacggga
    28501 aagaaaaatt actacgtgat gtagaataag cctttctatc gtttggttat tttatattca
    28561 aatatttgaa ataaataaat ctttactctt tgatttgtaa actgaaatga tccactgttt
    28621 gttttactga tgaagcttga tcaaaggaag aagctgagtt tgaagttcat aaaaatcagc
    28681 catagtgagt tttgcttgga tttgtttatc ttcttctact ttttacataa ttttgaacag
    28741 gataagattc aaatgtttta aatgaatctt tgactagttt gtgacagttg tcgcatatgc
    28801 tcaagcttcc ttggtcatta aattctgtat ggatattttt gttgttatta catgtacaat
    28861 aatcaggact atcgatcatc catttcgtct cctttacaat catcttgctt ctttactata
    28921 gcagaaaaga aggggagaaa caaaagaaga tccaacagac ggcactatag actgtagatg
    28981 ttgccatcct catccttcaa acggtaatta ccgatttttg taaatatgac tgttgtttct
    29041 tttttatttt cttttcctga aataatatat tcgtcatctt tggtagtgat cttgatatct
    29101 gtcagcactg ttttttcttc agatgattca gaagaatcag ttccttgtgt ctgatacgtg
    29161 cgtgtccatt ccattttttt atctgtaaat gtcaactgtc ccgcggataa atccatatct
    29221 ttacgtactt ttagcgtata tacactgttg gtggtatctg ctttttccgc ttttaagaca
    29281 gaagaatcgg ttgtttcgtc cgaggattgg tcagagccgc caaacaaatc acagccagtc
    29341 aatgtcaaaa gtcccgcaga caaaataaat agtacgatta atttcttcaa aatacttccc
    29401 ccatttgata ggataaagaa attataccat tggaatgtaa aacttcaaac ggatggaaga
    29461 gaaaaacgca caaaacaccc acaaatagag taaattttag aataaaattt taaaaaggca
    29521 aaaaatccgc cactttcaga aagcgtttta aaaaagtgtt gcaatctttt aaaaaaatgc
    29581 tatattaaaa atgaaccggt tcaaatactt gtttaagtat aattaatagt gagaggaagt
    29641 tgctctactg aagactttag ttatatggat attgatgaca agcaacacta tgcaaagaat
    29701 gcggtcatca taatcgaaca acaagaaaga gaaatcaaat gtaactggca ggagtgagaa
    29761 tgtatggcat cgatccgtga tgtggctaag atggctggtg tttcagtggg aacagtttcc
    29821 cgttacttga atggtcagca actgaaggaa aagaacatga aaaaaatagc agaagcgatt
    29881 tcagcgttgg actataaaga aaacatcatt gctaaagggt tgaagaataa tcgaagcttc
    29941 tctattggac ttttgataaa cgggatttcc agccgttttg ggtcggaagt tgtctcaggg
    30001 atcgaaagag tagctgaaga aaatgggtat agcttattat taagcgggtt tgccgatcag
    30061 ccagaaaaaa ttgaacaaaa aattgaatac ctgatgaagc atgtgatcga tgggctgatt
    30121 gtcttcttat ccgaagaaga gtggtctgga tttgaaatgc tcacaaaaat gagtattcca
    30181 gttatcgcat tgaattgtcc agaaggacca gcaggtattg atacaatatt agtgaatgat
    30241 cgagaaagtg tagcaaaagt gatcgctcat catggtgaac aaggacataa aaaaatcgga
    30301 tttattgctg ccactcaaac agactacgta gcaagagaac gtttagcagg agccaaagaa
    30361 gcggtccaaa atgatccatc gatccaatta gaggtcttta caggagacta ttctagaaaa
    30421 agcggctatt acggggcaaa agcattatta gaaaaagggg caactgctat ttttgtcagc
    30481 aattacaata tgtcgattgg tgccatcgaa tgtttcaatc aagaaggtgt cagaatcggg
    30541 aaggatattg cgttcagtca ctatgattat ttagataaaa acgatctgag tatccttccg
    30601 aaaatcacga tcacgccacc aacagataaa atcggggaac gagcagcaga acagctgtta
    30661 aaacgaatca aaaaagaaga ggaagaaaat gaaaaagtga ttgtgatgga aaatatcata
    30721 catggaatca actaaaacga actaaaaaag aaaaagaggt gctttgatga aaaaatatca
    30781 atttccagca aatttttggt ggggttctgc agcaagtggc ccacaaacag aaggaaggat
    30841 ttcaggcgat ggaaaaggag agaacatttt tgattattgg tacaaaaaag aaccgcaaaa
    30901 gttctttgat caagtaggac ctgaaaaaac ctcgcaagtt tatacgaagt atcaagagga
    30961 tgtacagttg atgaaacaga ctgggcataa ttcatttcga acatctatcc aatggagccg
    31021 tttgatacct gatgaatctg gaacaatcaa cgaagaagcc gttcgtttct atcatcgtta
    31081 ttttgatgag ttgattgaaa acggtatcga accctttgtg aatctgtatc atttcgatat
    31141 gccgatgtat ctgcaagaaa aaggcggatg gttgaaccga gaaacggttg aagcctatga
    31201 aaaatatgcc aaaagttgtt ttgaattgtt tggttcgaaa gtaaaaaaat ggtttactca
    31261 taatgaacca atcgttccag tcgaagcagg ttatttatat cgatggcatt atccagaaga
    31321 gtcagatatg aaaaaagcga tccaagttgg ctatcatgaa gcgttggctt cagcgttagc
    31381 catcaaatct taccatgaaa tggagcaagg tggtgttatt ggtatcatct tgaatctgac
    31441 accaagctat ccaagagacg aatcaaaccc tgaagatgtt aaagcagcgc aaatagcaga
    31501 tgcgttcttc aatcgttcat tccttgatcc agctgtaaaa ggcgagtttc caagtgaatt
    31561 gattgagata gtaaaagaac ttgatctcat gcctgtgatc catgcagagg atttgacggt
    31621 tatcaaagaa aatacagtag atcttttagg tatcaattat taccagccac gccgggtaaa
    31681 agcaaaagaa acaccaatcg atactaaaaa cggtccaatg cctgaggatt atttcgacaa
    31741 ttatgaaatg cctggtcgta aaatgaatcc ttatcgcgga tgggagatct atgaacaagg
    31801 aatctatgat attttgatca atgtacgaga tcattatgga aatatcgact gctttatttc
    31861 tgaaaacggt atgggtgtag aaaacgaagc acgatttatc aaagaagatg gcatgatcca
    31921 cgatgattat cggatcgaat ttgtccaatc tcatctgaaa tatgtccatc aagccattca
    31981 agaaggtgcc aattgtttag gctatcatat gtggacatgt atggataact ggtcgtggac
    32041 caatgcatat aagaatcgat atggctttat ttcagtagac ttagcacaag atggaaaaag
    32101 aacaatcaaa aaatcgggtc attggttcaa agacgtatca gataaaaacg gatttgaggc
    32161 ttaagaaaga atagttgaga gcaaatgcgt gaaattctcc attcttacca ttttgtgaaa
    32221 aagaagttca attatttacg atctttttca acttttttgt ctttctattt cattttttgt
    32281 atataataga atagtcaaaa ttaaatagga gtggaggaaa gcatgaggaa aaaaagaccg
    32341 tttcttctct tttggttatt cggttttttc ttactgctta ttttatccgc agcattttca
    32401 ggaaaaatga cagcagatct tgctcagaaa gaaacagaaa aaatcgtagc aagcaaagaa
    32461 tcagtagata atttcaaaaa agaattctca acttattggt gggcggagta taaaataact
    32521 gccaattacg aaaaaggcga attggacatt tatttgccaa aaggaaaaga agaaaatcaa
    32581 tttattaata tcgatgacgt acttagtgcg atttctctca gacaatatca atttaaagac
    32641 aagcatttga ccgttttgct tttgactcat gatggcgaca aagtagcccg tttgaaaaat
    32701 ggaaaagtga tcaccgctta ttctgaaaaa gtagacaaaa aagtggtcgc cgatcgttta
    32761 gcggaatgga aaaagacaaa tgaacaagtc aagaaaacaa ctgtctcttc tcaaaaaaac
    32821 gagtcgacag gaacaagtcc ttcagattct ggcagtggac aaacgataac gggaagtgaa
    32881 gaaaatcaag cctcaattat tacgaatgag tcatcacaag gacaatatca atcctatgga
    32941 caatgaaaaa cagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaacgg
    33001 caaaatagtt cctcatattt tgccgttttt tgagctaata c
//
LOCUS       DARBFD010000022        32486 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000022 DARBFD010000000
VERSION     DARBFD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.022"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            11..1648
                     /locus_tag="SCK08_000995"
     CDS             11..1648
                     /locus_tag="SCK08_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200905.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1793..2533)
                     /gene="truA"
                     /locus_tag="SCK08_000996"
     CDS             complement(1793..2533)
                     /gene="truA"
                     /locus_tag="SCK08_000996"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1200906.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2724..3359)
                     /locus_tag="SCK08_000997"
     CDS             complement(2724..3359)
                     /locus_tag="SCK08_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1200907.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3506..4747)
                     /gene="sstT"
                     /locus_tag="SCK08_000998"
     CDS             complement(3506..4747)
                     /gene="sstT"
                     /locus_tag="SCK08_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEG1200908.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4945..5943
                     /locus_tag="SCK08_000999"
     CDS             4945..5943
                     /locus_tag="SCK08_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200909.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5956..7974
                     /locus_tag="SCK08_001000"
     CDS             5956..7974
                     /locus_tag="SCK08_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEG1200910.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8034..8750)
                     /locus_tag="SCK08_001001"
     CDS             complement(8034..8750)
                     /locus_tag="SCK08_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEG1200911.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8977..9519)
                     /locus_tag="SCK08_001002"
     CDS             complement(8977..9519)
                     /locus_tag="SCK08_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1200912.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9524..9751)
                     /locus_tag="SCK08_001003"
     CDS             complement(9524..9751)
                     /locus_tag="SCK08_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1200913.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9830..11710)
                     /locus_tag="SCK08_001004"
     CDS             complement(9830..11710)
                     /locus_tag="SCK08_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1200914.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11899..12513)
                     /locus_tag="SCK08_001005"
     CDS             complement(11899..12513)
                     /locus_tag="SCK08_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1200915.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12655..13215
                     /locus_tag="SCK08_001006"
     CDS             12655..13215
                     /locus_tag="SCK08_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1200916.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13520..13834)
                     /locus_tag="SCK08_001007"
                     /pseudo
     CDS             complement(13520..13834)
                     /locus_tag="SCK08_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14088..15723)
                     /locus_tag="SCK08_001008"
                     /pseudo
     CDS             complement(14088..15723)
                     /locus_tag="SCK08_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16278..16841)
                     /locus_tag="SCK08_001009"
     CDS             complement(16278..16841)
                     /locus_tag="SCK08_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1200917.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(16856..17899)
                     /locus_tag="SCK08_001010"
     CDS             complement(16856..17899)
                     /locus_tag="SCK08_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200918.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(18032..18436)
                     /locus_tag="SCK08_001011"
     CDS             complement(18032..18436)
                     /locus_tag="SCK08_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1200919.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(18669..19304)
                     /locus_tag="SCK08_001012"
     CDS             complement(18669..19304)
                     /locus_tag="SCK08_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1200920.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(19570..20622)
                     /locus_tag="SCK08_001013"
     CDS             complement(19570..20622)
                     /locus_tag="SCK08_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1200921.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(20635..21573)
                     /locus_tag="SCK08_001014"
     CDS             complement(20635..21573)
                     /locus_tag="SCK08_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1200922.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(22024..22674)
                     /locus_tag="SCK08_001015"
     CDS             complement(22024..22674)
                     /locus_tag="SCK08_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200923.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(22774..23283)
                     /locus_tag="SCK08_001016"
     CDS             complement(22774..23283)
                     /locus_tag="SCK08_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEG1200924.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(23484..24353)
                     /locus_tag="SCK08_001017"
     CDS             complement(23484..24353)
                     /locus_tag="SCK08_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1200925.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(24373..25494)
                     /locus_tag="SCK08_001018"
     CDS             complement(24373..25494)
                     /locus_tag="SCK08_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1200926.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(25496..25891)
                     /locus_tag="SCK08_001019"
     CDS             complement(25496..25891)
                     /locus_tag="SCK08_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1200927.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(25910..26716)
                     /locus_tag="SCK08_001020"
     CDS             complement(25910..26716)
                     /locus_tag="SCK08_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1200928.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(26709..27497)
                     /locus_tag="SCK08_001021"
     CDS             complement(26709..27497)
                     /locus_tag="SCK08_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1200929.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(27511..27987)
                     /locus_tag="SCK08_001022"
     CDS             complement(27511..27987)
                     /locus_tag="SCK08_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1200930.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(28059..30716)
                     /locus_tag="SCK08_001023"
     CDS             complement(28059..30716)
                     /locus_tag="SCK08_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1200931.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(30735..31139)
                     /locus_tag="SCK08_001024"
     CDS             complement(30735..31139)
                     /locus_tag="SCK08_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEG1200932.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(31371..31628)
                     /locus_tag="SCK08_001025"
     CDS             complement(31371..31628)
                     /locus_tag="SCK08_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEG1200933.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(31753..32085)
                     /locus_tag="SCK08_001026"
     CDS             complement(31753..32085)
                     /locus_tag="SCK08_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200934.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        10233 a         6443 c         4914 g        10896 t
ORIGIN      
        1 ggagaaaaaa atgaaaaaaa acaaaaacca aataatagat atcattttta tgttatttct
       61 aagttttgct tacgtcatcc ctctattcat gtctaaggga atctatcata gtgtcaatca
      121 ggatacttac tttcatttat ctcgaatcat aggactagat aatgtatgga gttctccagt
      181 aaacttcaat aacttcgatc atcatggaac aatgatgaat attttttatc catggcttac
      241 tctttatcca gcttttttat tttataaaat gatgggaaat cttgttttaa gttataatat
      301 ctactatttt tttatcactt ttttaacaat ggtcgtttct tatttttcta tgaaacaaat
      361 aaaaaacaat cgttatattt cgctgttatt ttcgattatt tatacatttt ccgcctatcg
      421 tgccattgac atattccatc gtgcttcatt gggagaagct gtcgctttaa catttttgcc
      481 tttgatacta atggggtgtt acgaaattta tattagagat taccaaaaat ggtactggtt
      541 gtctattggc atgacgctag tagtgtatac tcatttactt tctgtcgcta tggtttctgt
      601 atttattgga ggaacgttgt ttctttcttt ttatttttgg gaccaaaaaa tcgctagact
      661 cttaagtctt ttaaaagcta ccgtcctcac ctttttttta agtgcgggat tcttaattcc
      721 ttttattcaa caatctagag cacaagaatt gaaagtccca ctgggaaaag aattaagtgg
      781 tatggctcct agtgatatgc tgacacatat tttaaataat aactataaca actatactat
      841 tggattattc ttatttctcg gattgattgg tgcttttatt tttataaaaa aattaactgc
      901 tgatgattta tttattttct ttcttggtgt atttgttctt ttttgttcaa ccaatttatt
      961 tccttggcaa ctttttaacc atacaccagt taagagtcta caatttgtct ggcggttaaa
     1021 tggtttttct tctttattta tagcctatac gatgagtata gtgatctatt atattttttc
     1081 tactaaaaat aattcatgga aaataatggt ctttacgttt cttgcactaa tattgcatct
     1141 ctcaggagta agcaattcta tccatgctaa caaagattca ttaacactta tagcacctga
     1201 agacgctgtt gctatagctg aaaattacaa tcatactgat tatgcaaaca aagagtcaat
     1261 ctatcatcct gatatgatta ataatgatca atatttacta ggtaaccagg aaataaatcc
     1321 aacaactcat tttatgagta acaaacttac aattgaatta gataattcga ataataaaaa
     1381 tactgtcttg acgacaccta tttatcgtta taaagggcaa gtggcttcta ttaatggcaa
     1441 actagtacaa acaaaattat ctaagtttgg cacaactgaa ttaactattc ctccaggtat
     1501 aaataaagtc gttattactt atcagtacac aaaactagca attgcttcaa gatatttatc
     1561 tatcgttact ttgattttat ttttacttta tcgatttact ttttctaaac aaaaacaacc
     1621 gagaagagaa gtacaacatt ctcactagat ttttatttaa gaaagacttt ttagctgtac
     1681 acataaacag gctaaaaagt ctttttttat atttctttaa tttgcttcat acattattta
     1741 acatcgatat ttatactgtg aaacatgcgt ttcatccatt atcaaagtat ttttatcgat
     1801 aataaacttc atccaaaaac agaccttgtg cagggacggt ttctcctgca tgttgccgca
     1861 ctttgctttc aaatatttca tcgattgttt ctatcggcat ggcacctgtg ccgatttcta
     1921 gtaatgtccc catgataatc cgcaccattt tatataagaa tccgtctccg acaaaagtaa
     1981 aatgaagcat ttctcccttt cgttcgatag ataattcatc aatcgtacgc actgtggatt
     2041 tttttgattt tttcaatgca gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc
     2101 aagctgcatt cagcttgtcc atatccaact cttgagggaa ataaaagctg tggtttcgct
     2161 caaatgctga aggaatcacg ttgttccaaa cataatagct gtattttttg cccaccgcat
     2221 tatatcttga atggaagcgc tcaggcattt cttcgattct tttgatgaca atatcccgcg
     2281 gaaggtaacg attcataaag tcgatcattt ctgccagtcc aatttctgtg tttgttttaa
     2341 aatttgctac ttgtcctcgc gcatgggtgc cagcatctgt tcttcctgat ccaataattt
     2401 ctatctcttc atttgtcatc tgatgcaata ctttttcgat ttttccttga atggttttat
     2461 cgtcatctcc aagtctttgc catcctgaat atctttttcc gtcatattcg attgtcaact
     2521 tgatgtttct catattttag tggctcctct gtcttccatt tggtcttctc tctacctttt
     2581 taagattgta gcacaaaata tagctccttg cttctctaag acagaagcta tacaagaaaa
     2641 ctgtggaaat atatatttca gaaaaacaaa aaacagccac cggatgaagt atccgctgac
     2701 tgttcaattt gacggctcaa gttttactgt ttgagatgca agatactcaa cctggcaatg
     2761 ggcacataat taaagaaaaa actgattctt tggcaaaaac ctattttatt cagtaatggc
     2821 caagaattta tagaaggaat tcttgccagt ccataaatcg ccgcaaaaag caaactgatc
     2881 acagacagtc caaggtataa gtggcttacg tccatttgcc cattttttcg atagatcaag
     2941 aaaagaagaa ctggaaacaa taaaaaacca ctgtaaccta aacctgatcc gatattatgg
     3001 acccatgtcg agacattcca cgaagattca tgggtattga tactgaacaa acctgtaaag
     3061 atacaatcac ctataccata gaacgcgatg gctgctgtaa ctagggtagc taacacacga
     3121 gatgtactaa caaatgcagc atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa
     3181 accacagacc aaataagaaa cgcttttctt acaggactgg aaacctctcc aaatacactg
     3241 atcacgctct tcatttgatc taattcagga taatacaatc ctaatacgta cggagtcaaa
     3301 aaatcactca gcacgcctaa taataaaaag aaaaaaccaa atttacgtaa aaaatccatg
     3361 atacgcctcc aataacaatt acctatgctt ttaattgtta ttataaacga ctttatcttg
     3421 atttaacaga aacctctatt tacacgaaca tctatataac tcattaaaaa ctcgaatcat
     3481 tttaaaaata ctaaatatct aacgttcaac gttcacttaa tccatctaaa caatctttca
     3541 atttgatttt ttccccactt cttttacgcg cgcctagctc tcctattgca gtaaacaaca
     3601 gatcacttga cgaatttaag gcagtttcca ttgaatcctg gataacaccg ataataaatc
     3661 caataccgac gatttgcatc gcgacatcat tagagatacc aaacaaacta catgctaatg
     3721 gaatcaataa cagtgaaccg cccgcaacac ctgaagctcc acaagcagct aaagcagaca
     3781 aaatacataa aaggactttc aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag
     3841 tcaacgtcat caaggaaatg gtaatcgcgg caccgcccat attgatcgta gcacctaacg
     3901 ggatcgatat ggaataagac tcttttgtca atttcaatct ttcagccaac atcatgttta
     3961 tcgggatatt tgcggcagaa cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac
     4021 aaaagaaaat caatggatag ggattttctt taataaacaa aaacgtgagg aatgggtaca
     4081 caactaatgc gacaaacagc atcgtaccga tcaaaacaag taaaagctgc agatactctg
     4141 ccaatccagc gattccagtc gtagcaattg attgataaac caatcctaaa atccctatcg
     4201 gagcaaatgc aataatcact tgtacgactt gtgacaacgc tatagatatc tgatccacca
     4261 actcttttgt cgattctgaa cgtgctcgaa aagctagtcc caacaataca gcccagaata
     4321 agacgccaag gtaattcgca tcaataagag attgaacagg attttgtgtg acattcgtca
     4381 acatagtaga taaaacactt tccaaacttt gcggtgcttt ttcagatact gtttcttgta
     4441 aagtaatagg aacagtaaaa attgaactta caataactgc tacaagtgca gaaagcaatg
     4501 ttcccactaa atataagatc aaaataggct tcacgaatgt cttattccca attttatgct
     4561 tggcaattga cgccatcgtc aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac
     4621 tgacaaacaa cgttcccaat attgaaataa atgtccatgt ggggaaaaag ataccaaaca
     4681 atattccgac taaaatacca acaacaatac gttgaactaa cgacatatcc aaaaacttct
     4741 ctaacaaatg accacccttt cacaatttta acaatcattg tatcatttta tgcaaagaaa
     4801 ctaaaggtat aattttctaa aaattctaac aatacaaaca accatttctt gctaattttg
     4861 tactttttct gtctacacgt tgttttttga gatattctaa gttaggatgt tattaatggg
     4921 caacttatca ggaaacgttt tcttatgttt ttgtccaatt tttttgtgag gtgtttattc
     4981 ttgaaaaaaa taaagaagga gagttatggc ttcctgacaa tctttgtcgc ttgtataatg
     5041 atggttttac agcgtatatt gatttcagca gtcactattc ctacatatgc aacaaaccct
     5101 ggtcgattct acatctatac atttttacaa acttccgttg ttattggtgc aaatttgatc
     5161 ccactttaca tgggttacca atatcaaaaa atcgcccgtt taaaagtttt acattattta
     5221 agtcgttttg cctttatatt cctaatagcc acactagttt gcaatatctt tttttacgta
     5281 caagctggca gcttgaatat tcgtgattat tggcttatcc taacacctat cagtcagaat
     5341 tactttactt atgctgtttc ttgtactttg cttttttgta gtattcctta tacagttgga
     5401 tttttaaata aattatcgaa aacaacaatt aaatatctac tattattttt aacaattgga
     5461 ttagttggct gctctactct atttaataaa gacccgtttg cattacagaa tggacagagt
     5521 attttatgga ttttttattt atttttaatg ggttatggtt tgaaagaatg gaactggaaa
     5581 gaaaaggtcc gctataagct tcctcaagta tttatcagct tgattgtttt attcggtttg
     5641 attattttga tgacacaaat ttcactcatc atcagaggaa atacagatac agcgctgagg
     5701 ttttctaaac catattctct cttcgcaatg tattatactt tctcttcgtt tttattttta
     5761 gaaatgctat ctgataaatt aggtataaaa ggaagaggaa attttcttgc tgtatttttg
     5821 attgtcactc aagttatatg taatttccct ttagtttctt atcgcgtatc tacatttatg
     5881 aaacgtgaat ttccaaactc agggaaggca tggctgataa atatcgggca gttttttatt
     5941 tgatatttat tagcagtgtc cctcctagtt atctttttat tttatataca aaaaatccgt
     6001 ttcattcatc gtttcatcga atacttcgct tttgattcga ttgaacaatt aactcagcgt
     6061 tggaaaaaaa gaattcattg gctttttgtt cataaaagtt attttttagt tgcagcattt
     6121 ttttactgct ttaccttcgt gcagattttc gttttagaac caaaagaagg ttggaaagaa
     6181 actatccagg ttgccttaaa aatatttact cagcgacaag cacccatgat cctgacaatc
     6241 atcattatta tgggattttt cttcttacta ttgttgattt ccaatcgttt ttggtacgcc
     6301 ttgactgcta ccttgatcat taacttactg ctcactattt cgacagtaat caaaatggaa
     6361 atgcgggaag agcctgtctt tccttcagat ttaaaaatgc tgaccggatt gtcagaacta
     6421 ttatcgatgg ttagcccagt cttattaatc gtgggcggat tgatccttct cctattattg
     6481 attactagta ttattgtaca acgtagacta caaaaacagt attccttgaa gattcattgg
     6541 aaacgtcgat ttatcagtat cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt
     6601 aatcacaaaa attcgccttc tttcttgctg tttaatttat ttaaagtaaa caagactttc
     6661 tttaaccagc aaggcgccgt aaaagataac ggaccagtca ttcaattcct aaacaatatc
     6721 gacattaaaa tcatggaaga acctgctggc tattcgaaag aaaaaattaa tggaataatg
     6781 aaaaagtaca acacaaaggc agatgaaatc aaccaaacaa gaagtgactg gttaaacaat
     6841 cagacgatcg ttctcaattt aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg
     6901 acagtaccta cagaccctat tccttacatc aaaagcatta taacagaagg aacaggagga
     6961 ttgatgttat ccgtcggtta tggtggcgga acagctaaca tggaatggga aggactgaca
     7021 ggcttgagta tcagtaacct gtctgcttcg ttagttacac cttataccca gctagtggag
     7081 cgtcagaaaa taagtccaaa tattaccgat ctattcgatg aaaaaatagc catccatcct
     7141 tttacagctg ctctttatcg cagaaaacaa gtgtttgata aatttggttt tgacaggttc
     7201 tattatttag acagtccgga taaattaact tatacggata aaatagaaca aagcccacgc
     7261 atatcggatg attcagcata caaagaaacc ttgaaagctt tacaaagcaa tacggaaacg
     7321 tcacaattca tccaattatc cactatgcaa aaccatatgc cttatgatga ttactatagt
     7381 gaattgaact acactgctga aggaactgct gtcattccta atagaagaaa agaattacaa
     7441 acttatatgc aaggccttca ctataccgat acagcagtaa aaaaattcat ccaagaaatc
     7501 gacaaaatcg ataaaccgat tacctttgtt ttttatggtg accatttacc cgcaatctat
     7561 agtgggaatg attcgaaaaa atatggatta gaacagcacc agacagatta tttcatttat
     7621 aacaacaagt atagtcgtga acaagcagct acgttaaata aagaagttgt tgctcctaat
     7681 aactttggtg ctatggctct tgagcatgcg aatatcaaag taactcctta ctatgcgttg
     7741 ttgacagaag tatctaatca tctacctgca ttaacaattg atcctacaga aagcttgtct
     7801 aatcgtttta atggaaaaca ggtctttgtt acagaggata gtcaaattat ccaagaaaaa
     7861 aatttaactg agaaacaaaa agaaattcta gaagattatc gtctgataca atatgatctt
     7921 gttgcaggta accagtacag tgcaacatgg gcgacacaaa aaataaaaga ctaacaaata
     7981 aactgagcaa ggggttgtga taaaagacat gtcacaaccc cttgtttatc tcattaattt
     8041 gcttgtccat aaacgatttg attaaagcca tttttcaatt cattattcgc tgtctcgttg
     8101 aagtaaagat aattatcggg attcccctga ttttgttgtg tcaccaatac ttttggctga
     8161 ttttgctgaa acccgaatag atagacgtgc tttttcagat atggttctgt ctctgcatcg
     8221 gaatagacgg ctgtgatttg atagtctgct tgacctgtcc cactttctga ccattctgca
     8281 gaaacaggca ctccaccaat agccatcttc cactccccgt tcaaaatagc ttgagggact
     8341 ttaagaccat ataagtccac actcatttga ttcgtataac ttttgtattg ttgtcccatt
     8401 gtttggcccc atttggtcat aaatgcctct aattgtgcgg atttgtctgc attccacagt
     8461 gtttgttcaa cttgttcgct gctttctgaa gaggaagatg aagcctctac agaagaactt
     8521 gaagtagatg tttgagttgt ttcagtcgtt ttggaagact ctgacgaagt agaggtatct
     8581 gctgtttctg tacttgctgc agttgacttg ctctctgtgc tagttgttgt agtagttgca
     8641 actgttgctt tcgctgtttc ttttgttgat tcttttttcg attcattccc gttggtacaa
     8701 ccagctaaca aaaaagctag accaattgtt actaaaatcc tttttttcaa ttaaaaaact
     8761 cctttcgatt actcccactt ctttattcta tcaaataagc aatgtgattt tattgatttt
     8821 tcgtattttc tcatcaatct ttcaaatctt ctaaaaactg agaagcaccc gctgatgttg
     8881 tctgtaatac atcagcgggt gcttctttta ttttaatctt cgtgattttc caatttacct
     8941 cacgctttat atttgtattt gaagaaacat agacttttag tcatcatcgt cttcctcatc
     9001 caaatcttgt cggacagttt ttccttggag tctttgtact tggtagatga tttctttatt
     9061 gaaacttaaa agttcaacaa gcaattgttg caagccatat ttttcttctt tttcagctaa
     9121 tttgattgcc cgtgtgataa agacattttg gaaatcaaaa tcttttgcaa agttttccaa
     9181 cattgttgag gctggtaaat atttgttttg accatgttcg taaatttttc cataacgcat
     9241 gacttcttct aaagtagacg gaacgatttc attttcaatc actaactcat gagagatttt
     9301 attgaaccat ctcgcttctt gatcagcata ttctgaaaaa agggtactta attcttgcga
     9361 agatgtaccc aaaacataca attgtgcttg gatcaatttc atttcttcta atttaaaatt
     9421 tgctaataca tgccccacca tggcgccagc agttggttta tgatggtcga tatctgcttg
     9481 ttttgtttct tgaacaaatt tttcttccac tatattcatt gatttacgcc tcttttactt
     9541 ttgctgattg gacttcataa cctaattttt ccacaactgc agctaattgt tcagggtgga
     9601 cttcatctgg atcaaagttt gctttgattt tgctcgcatt aaacaaaact ttcgtattgc
     9661 ttaccccagg ctgttgagaa accgcatgtt cgattttttg catacagctt ggacaagcta
     9721 gtgtatctaa ttgcataata atttttttca tcgtagttct tccttccttt ttcttgatag
     9781 tcttattcta gattacagct tccataaaaa aattgattct tatcaagttt tacgattttt
     9841 cttctttgct ttcgtgtcca aaatacaata aacgcatcgc gttaaagata acgatcaaga
     9901 tactagcttc gtgaacgaac atcccactag ccatgtaaat aaacccggca aataagccgt
     9961 tgagtaaaaa taagacgaca ccaatggcaa tcactacatt ttcacgggta tttaaaacag
    10021 ttttcttagc caagcggtag gcatgaacca acgattcaaa attagattgc atcaagacaa
    10081 cgtcactcgt ttcgatagca acatccgttc ctgatcccat agcaatcccg atatcagccg
    10141 cagccaaaga gggagaatca ttgattccat ctccaataaa agcaacgcgc aatccttgtt
    10201 cttggtattt tttcacaaat tgaacctttt cgtctggtaa taattcagca tggacttcat
    10261 caatgcctaa catatcagct acataatcag cagtcatctg attatcacct gtcagcatca
    10321 ctaactgttt tgctcctgct tgtttcaact tagctaattg ttcagcgact tctggtcgaa
    10381 tcacatctga cacacctatg atttgagtga tttttttgtc gattgctaca ataatgaccg
    10441 agctgcctgt ttgttgtaat tgatgtaaat cttgtacttg ctttggtgtg agagaaagtt
    10501 tgtgagcggc aatcaatttg gtgttgcccg cacaaactgt atgaccatca atctcagctg
    10561 tgattccttg tcccttgatt gtctgattat ctttgactgc taattgttga aaatcaatgt
    10621 ctttctcctg agcataatgg atcacggcgc gccccagagg atgatcggat aacgtttcta
    10681 atcgagccac taaagctaag ctgtcccctt catcctctgt atagttcttg atagtggata
    10741 cagcagtagt tcctttggtc aatgtaccag ttttatcaaa gacaaaagta tcgattttgg
    10801 aaaaagtatc cattgcttca ccgcctttga ctaagatgcc ccgtttcgct ccattaccga
    10861 ttcctgctac attcgaaaca ggcgcaccaa tgactaaagc gccaggacaa ccaagaacca
    10921 aaacagtgat tgctaaacga aaatcacgac taatcagacc aacaaccaac gcaataagta
    10981 aaacggttgg agtataatac tgagaaaatc gatcaatgaa tttttccgca tgggatttgg
    11041 tatcttgtgc ttcttctact aattcgataa ttttggcaaa ggtcgtatca tctccgacct
    11101 tagttgcttc aatcgtcacg tagccatctg ctaaaatcgt tccagagaag acttcatctc
    11161 cactgatttt gacgacttgt cgtgattcac cagtcactgc ggcttcatcc gcataccctt
    11221 tcccgtcaat gactcgtccg tccacaggaa tagctccacc agtttttact aagacgatat
    11281 cgccttcttc cacgtcatcg atttccactt cttcagtcgt tccatcttct tggacaacac
    11341 tagcagtaga tggggccatc tcggtcaaag ccttgattga ctcccgcgtt ttggtcaatg
    11401 ttttttgttc cagatagcta ccaaacaaaa acaagaatgt aacgatagct gactccttgt
    11461 attctccgat cataaaggca ccaagcacag caatagtaac gagtaaatca attgagataa
    11521 ctttgacttt cactgcctga tacgcatgga tcataatagg tatgaccgca ataatagaag
    11581 ctatggctag agcaatgtta tagaacaaga caaaaccagt aaattttccg ataaaaccaa
    11641 aaataatcaa cacagctgat aaggcggtga tatgatttcg atgctcttgg atccactttt
    11701 gtaatttcat ctttctccct cttttccttt cattcttacg agactaagta tagatactta
    11761 tccatcataa aaaattgacc actatcaagt ttagtcaaaa aggcggtttt cttttggtag
    11821 aatacactac aaaaaatagt tagcaagaag actcactaaa agtgatcttg ccaactattg
    11881 tcgtttctat cgaaattttc attgaatgat tttcctgtac agcatacatc ggagatctat
    11941 ttctttatct aagattttta tcttgctaat tttttccttt agtctttcaa atccacgtcg
    12001 ttcatagaat tgatacgtac agccactatc tgtaaaaagg aatatttctt ttcctttctc
    12061 tcttttttca aattctgtta gcaacttggt accgatgcct tttgttttca aatcaggatt
    12121 tgcagctaaa aaaatgatct ctccatctgg cgaatgcttc tttaaatatg ctgcaagcat
    12181 ttgttcattt gctgtctcgt aagctccttt actttcttta gcaaatatat tttgtaaaaa
    12241 atcgaatatc ttaacatata tttgctgtcc aaaagaacga tgtttctttt tttctccttt
    12301 tatctccgcc agcaaaacac ctgccaactc gtcctctgca tacaaagcaa gaatttgagt
    12361 tgctcgattc aactccatat acaaaaaata tcttccgtaa agattcagca gaagttcatt
    12421 ttccatatat ctctcaaagt tcattccttc aacagcaaaa tagatagctt ttttgtaatc
    12481 tttcttttct aagggtttaa aagttagttt catcatttcg cctcctcaac ttcaatgatt
    12541 gtgttatact tacattataa tagacacagt gtctactatc tataatcaat catctataag
    12601 tttgtcgatt aagcgacagc tttgctttga tatgtcggat aaggagatag gtaaatgaaa
    12661 aagacagata gacgcgtcaa aaaaacagaa aaagctttgg ctgaaacact atccacatta
    12721 ttagtaaata aaaagattca agcaatcact atacgcgaac taacagaaac ggcagatgta
    12781 catcgttcga ctttttatac acattataag gatatctatg atctatatga tcaattagaa
    12841 agcaactttt ttcgggatct aaatgaaatt ctctcttatg atccagccca tagctatgag
    12901 gaactataca ctcgcttgat tgactatgtt tacgcaaata cttttttata tcgcttattt
    12961 atcctcaacc atggtgatat ttcgtttcag aagaaagtta aacaagttat tgaagaaaac
    13021 tatctgaaca tatggctata tgaagacaag aaaacgatta tcacagaaga aatgagattc
    13081 tttactacgt accacgtcca aggttgtcta tccgttctca atcggtgggt agaaagcaat
    13141 tacaagtact ccaaaaaaga attattagaa ctcttacaga aactgaatga tcacatagag
    13201 aaaatcatgc cttgaataaa aacatactaa gcttagttaa gaagaaccgt aaccttctcg
    13261 tataacaact gaatcttatt tagccaaatt gtttactgaa gattttttga gtaaaatttc
    13321 tataattcct acttattgaa aagtagtttg tagtgctgct tctccctctc taagtgatat
    13381 atcttgttgt accaatcaaa ttcacctcat cattaataaa ttacttttag gctaatttat
    13441 tacaacttat aggttgaagc tgtaaccaat ttatagtcat attatatttt tatcatccag
    13501 tttattgaat tcttctcgtt tacctcaatt cattttcaag gggaatcagc tcatcatcta
    13561 acattttacg ccatgctcgc ttcatttctt gtgggtattt aaatggtttc gcttctggat
    13621 gtttggcttt atatccttcg taatccttac tgttgataat tgctttcata ttaggaattg
    13681 gatcttcacc tggcatatat tgcagattag aagcatgtaa ttttttctca gaaacaaacc
    13741 actcattgga aaagacatga atcaaatcat cacttacttg tgtaaaatag cgacgtttaa
    13801 tgacaaagat atcttcattg ctatctagtg taccttcatt tctttgtgct taaattcaaa
    13861 gataattttc tcaataaaga tttttgattc cagtaagatt cctttactcc ttttacaaca
    13921 tagcgttgtc ctttgttttg aaacatcttc ttcccctctt tgccattaaa aatagcccaa
    13981 ttctctataa agaagattaa gcttcattat tataatataa agtttaaatt tggtttatta
    14041 cttacttttt cttattctct agtaagctaa tattgaaaaa aatgtaatca gatcattatt
    14101 tttatatgtt gtaacttcct gataagaaaa ctaattaaaa gaaagaggta actacataaa
    14161 ctaattaaaa aagctaactt catagttgga ggataaatgg aagaaacttc aatgattgtt
    14221 tctccttgtg ttccttcaat cgacactagt cctattatca atctctcttt tttgtccatt
    14281 tacttttgcg actgttcccc aataatcaaa tacaggaagc gttatttttt gtcctttaga
    14341 ttcatgtact gtatagacta ttttcgaatc catcacttga taaggcatag tatgccattc
    14401 cttgccaatt cttacttcat gattctgcaa atgttctttg tattgtaatg cttccgtggg
    14461 tgcatagtca taaagtcctc cgggaaggag tgtcgtgtaa cgaataagat tttcttgatt
    14521 cattaccaag tgatctctct gagtgacaac tttatgttca tagtttgctt ttgttgcagc
    14581 agatacagcc aacagtaata aaacaattgc cgttatatta actacttttc tttgcttagc
    14641 tgatgtgctc ttcatgaaaa cgaacgataa aatcaacgag ccaaacagta cttgaaagcc
    14701 taagattcgc caaggaaatt gaatcatttg aatgggtgta ttttgtaata aagaccatgg
    14761 aaaaaatttg gtggataaga gccaagagac taccgcacta aggtaaatca ctttttcttt
    14821 gcgattaaat cctttaaaaa agaagagtgg acagatcaaa gaaataatac caatgattcc
    14881 taccgtaaag gttgtcaggt cattattcat cgcaccaatc aaactatccg agacatttaa
    14941 tgcttggtgt tgtaaggtcg ttttatctgg ctcattaatc gattgaacca tgatttgtcg
    15001 gaacatcgga taccaaaagt atgctgttaa aagaatcgtc attcctactg cctttgacaa
    15061 ctccacaatc aaccggatgc tttctttttt tcttgtttca cttactacaa agccgcctaa
    15121 gactagtaag ccaacggcta ttacactcat aaaagtggaa agaacatgtg tataaatgag
    15181 taagctcata ctgactgcta atggaattgc acatggcttt ttctcataaa tcagtgcgta
    15241 agcataatac ataactatcg gtataaccgt aagtgcaatc gcttcagcaa tagcagaacg
    15301 atgataaata tcaatcgtac ggtacccata aaatgtatat aaacagctaa atataaaagc
    15361 cgcatcgatt ctttttaaaa attttagtcc ataatgaaac atcagcatga tagtacaaac
    15421 attcaatagc caaacatata agatataact aacgattaaa ttattactta tccaataaaa
    15481 aatgactgcc ggaaaaaagg tcaagtaggg ataaaaataa ttcactccgc tcccataatg
    15541 attaaacgtc gtaaaattga ttggaccaga aaaaattgaa gataaccctt tgactctagc
    15601 aaaatggaag gcaatatccc ccgatgtaga aatagtatga acatttctaa aataattgat
    15661 tgaataaagt ccagcaataa ttgctaggag aatggacaat attatttttt tctttacttg
    15721 cattacttaa ttatatactc ctttcatgga ttgctttatc tagtataact taaattttaa
    15781 ataaatcgtt atttattttt actctcactt aaaaaagcac taaaattata agagagggag
    15841 gtaatggtct gaacaccaag aattgttcca acatcgaatt gcttaactaa tgagttcaaa
    15901 gcttaattat gtggctctta tatttaccga gcgtggattg cgatctcttt agcgaaacaa
    15961 acatctttca taaaaaaaac agccatatcc gctttcttgg atatgactat ttactactac
    16021 tttactatct ttcattcttc ttgtctcact tatgataagg cgctgcgtat catgagtgaa
    16081 tgatattagg aaaacttcat aaaataaatc ttttatacat ttccacttct agatggtcat
    16141 tcatgttaga agaatcaaac tattttttta ctgtcgtaat aatattaaaa cactagaaca
    16201 aatcaattaa attccataaa atataaataa tagacctagc atcatctggt ataaaatgga
    16261 tgctaggtcc gttatattta ctcaaaattg attctacgaa tagatcttct tatcatttct
    16321 attttagtag tcatgcgttg ttgttcttct aaaggtggta atggtataag tagtttagat
    16381 aaatttgtac catttacatt tggttgccca gttccgtaag aaattttttc taataacttc
    16441 caatataatg gcgagtctaa gaagcagtct acgtattctg ataataattt ctcaactaac
    16501 ctaattcgaa tcaagtaaga agcaaaaaca gattcctcac ttatttcctt aaccaagaaa
    16561 ctttttccca tagttcctcc tgtccttgct atcaaaatgt cattttcttc caatcttaaa
    16621 tcaactagtt tagaattact aatatcacag taaggcacac tggaccaatt tacacgtccc
    16681 tcttgtatat ctgtgattct caaaaactta acattgcctt gttttttcgc cgaatctgta
    16741 tagccatatt gaatcttagt agaaatactt cctaaataaa cccatagtcg aacatcgcta
    16801 gatgattttt cttgtcgttc tttatgaaca acagtgatca tcttgagttg tccgactatt
    16861 ttttagttgc agcaatcttt ttgggacgct tatttgcatg aagcaacgtt gctaagtcta
    16921 gtaataaaac attggctaac acaggattgg ctttctcgcg atacgctgcg gcgccacttt
    16981 caaacaatac tgctagacga tcttctacaa caacgatttg ttccaaatac ggtggaaatt
    17041 tgattcgttt taggatccgg ctaggagagt cccgctcgga cactaagagt tccgaggtat
    17101 aaggaccata agaacgactg gctaagagat aatggccata aaaagccaca ccttgaatct
    17161 tatcagatgt ttttctgctc attgtcacgg ttggttggat tttgtctttg cggcgaattg
    17221 ctgttttcat ttcagctatc gtcggcaata gatagaaagt caactgtcct tcacctttta
    17281 gtgtgaagtt gccagatacc agttgatcat tgttttgagt caaatacgat gcttgtggaa
    17341 tctctgagag atcagttgtc agccaataag cgattggctg tcccgtctct tcagatgtat
    17401 ctgctaagat atcgatcaaa cgtaaagcgg ctactcgtcc agtagcagat cctgtgattg
    17461 tcaaccataa aagttcccgt tttgtatcat atactaagcc ccctacatgt ggccgtttag
    17521 gtaaaatcag cagtttgatc ggctgtcctg tttctctatc cagtacatga ataatagaat
    17581 gatgctgatg gtcatagcag taggcagaaa taaacacaaa atcttgagaa aaagccaacc
    17641 cttgcggcgt catccattga caaacctcat tttcgccggt ttccggcacc accattcttg
    17701 tttctgttaa tccaggaatg ttatattgtt ttgcttcagc cttttcccaa gctgcttgaa
    17761 caataggata ccgttttaac tttttttcaa tatcctgatg tatccgttgc tgcttccaat
    17821 gcttaaactt taggacatag taaacaagaa ggccaatgac cagcaacact aaaatcatta
    17881 ttgctacccg ctgtgtcatt tttttgcctc cttgttctat tcgatcaatc gtccatatac
    17941 cgctattaat taccgtaact acatgtttct tttactagcg tttctttata ggatctttca
    18001 taatctcatt tatagtaaca ttaagaatcc cttattctac tatagtcact tccacatcaa
    18061 tattgcctct tgttgcttta gagtatgggc aaaatgcgtg ggcttcttct gcgattttct
    18121 ctgcttggtc cttctcaagt cctggcaaag aaacttctaa aacgaccccg atttggtagc
    18181 ccccatcttc taattgaaat aaagataccc gtgctttgac ttcagaatca gactcaagat
    18241 tattttcttc cagcatgtgt tgaaccgcgc cgttgaagca agatgcgtat ccagccgcaa
    18301 aaagttgttc cggatttgtc ccttcactat ggattcctgg gcggtcgatc ttcatgtgca
    18361 tacttccgtt tggtgcctca acttcgccat cacggcctcc acgattgatc attgctgttt
    18421 cgtacatttt tttcattgag atcatccttt ctttcgtcgt tctatattaa gtgtaacgag
    18481 tattttttaa tctgacaaag gtaatgcttg cattcggcga catcagttag acaacatcga
    18541 tgatcagtga tagaagcagt ttatctatac aaaaaaagaa gggttctatg atatttggtg
    18601 tccttactcg ccttgagtaa caacaccaaa tatcatagaa ccacttaatc agcttcacac
    18661 tgctttttct actttcgtag ttgaaaaaaa cttaaacaaa tcatcggcaa atagttaaag
    18721 aaaaaactta atcgttgaaa gaagcccaat tgcttaaaga aggcaaactg actaatcgac
    18781 ggaatccgtg ctaatccgta aagacctgca attagtaaac taataactgt taaaacaaga
    18841 taaaaatggc taagcgtacc ttgaccactt tgacgataaa gcagaacaag taacaatggg
    18901 aaaagtagaa agcctgcata gcctagccca gaacctatat tatggatcca agtcgataga
    18961 ttacaagagc tttcattcgt attgatactg aataagccag taaaaataca atcacctatc
    19021 ccatataaac caactgttgc aacaacaagt attgcaagtg tttttgatgt tccaacaaat
    19081 aggtgataca acgcaggcaa cgaaagaaca aaaaaaagtc cagatacaac tgaccagacc
    19141 aagaaagcac gtctgactgg actatctacg tcaccaaaca cactaattac ggtagtcatt
    19201 tgattcagtt tgggataaaa aaggccaagg atatacggtg tcgataaatc actcatcaca
    19261 cctaataata aaaaataaaa accatatttt tgcaaaaaaa tcattttctt cccctacttt
    19321 ttagtgatta ttcgatacat ccaacaatca aattttttct cattttaaca catattttta
    19381 ttctagttgt cttttgaaca atcaataaca taaaggtgag tttcttctac attttaaaaa
    19441 atcgaatggc actgaagagt tcagttgtca gcatcaatct cctactcaaa aaggcaaaaa
    19501 aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa aatttcttag
    19561 taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt taaatacttt
    19621 ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc aacaaagaac
    19681 aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa gtccgaccac
    19741 gccgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag gaatagctgt
    19801 aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata gattaggcat
    19861 catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat taaccttcca
    19921 agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg caccagcaga
    19981 aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag agataatcaa
    20041 aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg tagtgaagtt
    20101 attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata aataaaaacc
    20161 aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca ctgacccaaa
    20221 actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca tcgtattaat
    20281 aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag gtgtgaattt
    20341 gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca tcggatcaaa
    20401 tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctagtt gaaaaataac
    20461 tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta aaatggcacc
    20521 taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag cgaggacttt
    20581 attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc tcctttattt
    20641 tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac aataaggcgt
    20701 cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat cgatttctgt
    20761 taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc caattgtttc
    20821 cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt caatcttctc
    20881 tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat gagcagtcga
    20941 tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt tcattgttcc
    21001 gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat gagcatttaa
    21061 tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc catcacctaa
    21121 attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac cttcaagacc
    21181 tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt tctcaataat
    21241 atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt tgattaatga
    21301 ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa taatcagatc
    21361 aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg cttctacctc
    21421 tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat gttctgccca
    21481 gccatcgatt aataatactt cgttcccact ttggtgaagc atcaacccaa agcgacttcc
    21541 cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt tctttctatt
    21601 atactcattt gagaatcttt tcacaacctc gttttgttat tcttctctta tttatcatgt
    21661 ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa tagaatccca
    21721 atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca tagaaaaaat
    21781 ctcactcctc tgattataaa agagggggca ttaacctcgt gtatactaaa accttccatt
    21841 cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga tgttgcgcaa
    21901 cacagacgga tggtattgct tattatttat taaaatcgaa caatacactt atttaaaaac
    21961 ttctttccca gattttcttc catttttaca gttataacta gtattatcat gaaaaatagc
    22021 cttttattgt ctatgggaat aataatttaa aattggtact tttttattaa ttagttcatc
    22081 gcccaataat ctctccagtt tcttagcatc tcctgactca accggaatta tccaagccca
    22141 gtctaaataa ttttttaaaa tctcatcata ttttgcaaat atttcattgc catattccgt
    22201 ccttaaaatt atattttcag tgccagacaa gtgatttata ttgagttgat aaaaaagatc
    22261 tccatctaaa aaaatatcat tagaagaaga tttccccaca gtaatgacca ttccatcagc
    22321 tctatgtgtt acatacaaat aattgtatga cctcatttca cctaatgaat caattatttc
    22381 ttgcccttta gctctaatat attgatctct attacctatc tcactcttat ttaacaaaag
    22441 ttttttcatg tcaaatttaa tcggctgttt tatttttgta aattcgcttc tactactaat
    22501 ccaatccgag attaattttt caacatgtcc cagagtaaac tctgatttat tcgctaaata
    22561 ctttttttgt tgtattaaag acatctttcc ccgaagctca aaattgagcc tacatcgatt
    22621 ctctatctct tcaaaaagca tcttatcttc atcattctta tattcatcat tcataaaacc
    22681 aatccagctc ctcgattaag ataatatttt aatctcatac aaaaactagc tctcatcaaa
    22741 acaaccattg ttttgatttc tcatgaaatt tctttatact tttctccgat tataaattcc
    22801 taaccccttg agaaaattac gcatatattt atctccgcaa ggcttaaaat ttcgttggcc
    22861 ttcttttctc aaaaatgccc cgatttcgcc ttttgatgga tagatttctg cctcagcaaa
    22921 aacgtctaaa atatcatcag tggtcataga caaggcgatt tttaattttt ttagcaacac
    22981 attgttgata tatttgattt ccttgctttt ttgcggcgtg tctactatcg gcttatcttt
    23041 ttgcggacca cgcgccaaga gaatcaggcc atttaggaat gcgtcaaaaa cttggttgtt
    23101 gatggttttt tcatacacat tattttctgc attatctttg ttcttatcct gtactttggt
    23161 cagtaccgcc tgagcttctt ctttagtcag tggtacaccg cctagctcaa aagctttaat
    23221 caaatccgta tctttcaaat caatcgcata acgcaatcgc aataacctat cattgttatt
    23281 catttgttcc tccgaagtat ttagtcttta ctttcttacg tactttttgt cttgctaaga
    23341 tttttaggat caatgataaa aaccgcatca cttatgataa ggcgctgggt atcatgagtg
    23401 aatgatattg ctgaatacaa atagtccgac cacattaagt tgatcggact tcataagcat
    23461 tttattgact aatacttttt tgcttaagaa ttgattctaa catcttttgc taaagttaag
    23521 catcctatag ttcctgcatt atctgctaac ttaggtgtga caatgtattc ttcgatattt
    23581 ggcgtatcga catagccgtt aattagttct aaaaaacgtc tttgtacttt ctttttcaga
    23641 tgttcttgtt gcatgacacc accaccaaaa attataatat ctggtgaaag catcagggtc
    23701 gtattacagg cacattgtgc aatataatcc gcctcgattt cccagaacgg atcttctata
    23761 gaaagatctt gtcccttggt tcccgtacgc atctcgatag cgggtccaga tgccaaccct
    23821 tcgagacaat catgatgaaa aggacaagta ccagagaaag tatctcctga atgtctctta
    23881 actaacgtat gccccatttc tggatggcta aacccttcaa caaaaatccc atcttgtata
    23941 gctcctccac caattccagt tccaacagtg taataaacaa cactggataa accttttcca
    24001 tgacctgaaa catactcccc gtaacaagca gcattcacat cggttgtcca ggcaattgga
    24061 atttcgaaat gcttattcaa cgttccaaca aaatcaaaat ttttccaggc agtttttgga
    24121 gtagaggtga tatgtccata ggtctttgat tcacgatgga tatcaatcgg tccaaatgac
    24181 cctactccaa tgctttctaa ttgttcttta tagtgattaa agaaatcaat taccaaactc
    24241 attgtttcat tcggtgtagt tgtaggaaaa ctcactcttt ctaaaacctt taaatcatca
    24301 ttgccaatgg cacaaacaaa tttagtccca cctgcttcta ttgagccata catttcttac
    24361 tccaccgtcc ttttatctag gaattcgttc ttccctgtct aatagttttt cgaaaaactc
    24421 atgtggttcc ttttggtacc aataggcaac agaagaaata tcgtcttgac gctcataaag
    24481 cttgatgtca tcatttccta gggtttggaa agtaacacgt aaattttctt tgaaactaat
    24541 ggggtcgggt aaatgccagc ggtacaaccc atgcatcggt aaagcatcat cgccaaaacc
    24601 atgaactgga tttggattag gttttccagc actaaaataa tcccgggtgt gatctctttt
    24661 agattgaaaa ggatagccca taaatagcgt attaaaattt tgagcatatg ccctttctcc
    24721 aaagttcttt ttatggaaag cccaagcgcc gccaaaataa tcttctgctc ctgtagatgt
    24781 tattgtaggg aaatcttcat ctccatctat ataaaactta aattctcctt ccccccacca
    24841 gtatctttcc aatgcgcaca aagcaaagta ggttcctaca taatacccag tgcctttgat
    24901 tccatcaata agggtgtaat cttttccttt tgtcgtgatg ggttcacggt tccatttagc
    24961 atgaaaatac atcgcatcat ccggtacttc agtcaatgca taattaaatg tataaaaaat
    25021 gtgcttaata aactcaggat gttcattcgt taaagtaatc ttagcttttt ttcgaaatgg
    25081 catctcaaaa tagcaattca tccctgatgt tggattcact acgattggta aggaattcac
    25141 ttcgcatctt gtcccaaacc cattacaaaa aaaatctcct aatggacttt ctacagaagg
    25201 agtggattca tcatcccaat aaatacgcaa aattaaatca cgtaagacga aactcccagc
    25261 gattgttttg tctgtcactg tcatccaaaa atgacgtaaa attccagggc cttccatttc
    25321 tgctaaagta attgtctctc cagcatttat cggaacagaa ggtgaccctt ttctcccaat
    25381 tcctagatga ctcgctgtca ttgccgcttt ccctttttca ccatttttat tttctggtga
    25441 aatagttcta ctttcctctc ccttgtggag ccatatatta tttaaacaat tcattttatt
    25501 cgtcctctat cctttcttca cttttttgat tattcatata gatgatttgt tgttgtgcct
    25561 ccgcaataat tgctccaata tctaaattat catttcctaa ctgcaaagcg attgacattg
    25621 ccaagggaag attcattcct gcaattacgt gcactctgtc agaaatatag gtaaaaaact
    25681 tttgatttac acttcctcca gccatgtcag ttaaaacaat tatttcgtct gtagtatcca
    25741 ctaactcttt aatttgttca tctatatcat caatttcttc gacatatgca cataagctac
    25801 taatttgatt atgcatacct gttaaaaact ctagtgtctc tttaaaaccc tcagctagta
    25861 ttgaatggct agcaatgatc aatcttctca taattcaaat ccttttcctt tacgctaata
    25921 ttccaaatgc agccataacc atcgcaaaaa caataacgag tagaatcaaa atggtcatac
    25981 tgattttttt cttactgagt agataatatg ctgcagcagt aataattact ggaattaatg
    26041 ttggtaaaat tttgtccaac attggttgga ttttcatact aacttcacca aaagtgaatt
    26101 taagtggcgt tgaaacactg ataactgaag caattaatga tccaactact gttagcccca
    26161 atactgaagc agcttcagtt aaataattaa tcttttcacc gtattcagta acaatcttca
    26221 ttccctgctt ataaccaaat tcaaagcctc tcattcggaa aaagaagatt accatgatta
    26281 aaatgaacca taaaaagatt cctacaggat tattttctaa agccatataa gccgcaattg
    26341 atcccatgat ggttgggatc atcgtcatca aaagagagtc cccaatacca gcaattggac
    26401 ccatcgtacc aactttaaag tcattaatcg catcttttgc ttctaatcct tgcttttcct
    26461 ccattgcaac acaagcacct aaaataatgg ctgccagcca aggctgtgta ttgtaatact
    26521 gaaagtgatt atttaaagct tcgatgtatt catcatcatc tttgtataat tttcttaaaa
    26581 taggtgacaa tgcgtaagtc acagatgcac cttgctgtgt ttgataatta aaggtacaaa
    26641 ctgacatcaa ccaacgatag ttcgctttcc taagatcctt tttcgttact tgatattttt
    26701 cgctcatttt attcatcctc cccaataaac ccaacatcag aagctcccgc agaagcagtg
    26761 atttgtgcat ctttttcagc tctatgatac acattaaatg ctaaagcgaa tcctatgatc
    26821 gccgctccaa taataggtac cttcaaaaaa gcggctaaga aaaatccaac aagtaaataa
    26881 ctaatgtatc gacctgtagg cataaagcgt aacaacatcg caataccaac agcaggcaac
    26941 atttttccgg ctaatgttag cccatccata aaccaattag gtaaaacatc caaaacgtaa
    27001 ttaacagcag actgtcctaa agtaacggca ataaatactg gaattgctgc agataaacca
    27061 aagaaaattg gccccaacca caaaacacta ttcattttct taaattctcg tttattcgca
    27121 tattcctgtg atttgcgaac aacaaaacca tttaagattt tgacaattac atctagttgc
    27181 acccctaaca tacctacagg tagtcctatt gctaatccgg cttctactcc aagctggcta
    27241 ccaaatgccg ttgcaataat tgcgcacgtt ccatagtccg gtacagatga gccaccaaaa
    27301 ccggcaactc ctaatgacat caattgaact gtaccgccaa ctatcaaacc tgtttttata
    27361 tcacccatca aaattcctgt gaataatccc caaaaaactg cgtaatttac aaagatcatc
    27421 ggaatcccat aataatcaac atgtttaata aatgctaata tagtcagcat aataatttga
    27481 aaaatagtaa aatgcattat ccttactcct ttatcctaat tttgattgaa tttcctgtgc
    27541 cttatccgcc ggaacaaatt gtgtaacaat ttctacacca gaatccaaca attttttata
    27601 cgtgctgata ttttcatctg atgcataggc tctagaagat agctgtaatg cattatcttt
    27661 aacaacagta ccgccaacaa ttagtcggtc aattctaata ccgttatcta ctaaatgaag
    27721 gattgtttcc ggctctttca cgatgacaaa tactttttcc tgatcgtatt tccccttttt
    27781 aaaattcgca atcgctgttt cttcagtgat aattgataca gccattccag caggtctagc
    27841 taaacgcata cttgattttt ggatgtcgtt atttgcgaca gaatcatcga tcaccatcac
    27901 tctgtttgct ttagacattg gtgcccattg cgtgacaata atcccatgca ataaacggtt
    27961 atcaactctc gacataacaa tactcataaa tacttcctcc tttatctttc attttcttac
    28021 aagcaattac catgccaaaa caaaaagaac ttgataaatc aagctctttt tacataatca
    28081 taaacgtatt taacctcatg aatcgggatg tcgacactat aataatgttc aacgacactg
    28141 aaagattgct taaaatgtgt ctgaaatttt tgaaattcct cactcgtttc attgaaataa
    28201 atttcatctt ccaaagtatt ttgttttact aatcgttcaa ttaagcaact aatgtgaaca
    28261 tataatccga aacacgtatt attatcaaat gaaattccta gatcattttg taagatgtat
    28321 atagcatctg aaacccgttc taataattta gtcggattta atatggtcaa ttgctccatt
    28381 aaattcgtta gactaaaatt cttcagaata ttattgttca aaactttcag ttcattatca
    28441 ttaaagtgtc tattcaattg attggagaat ttacttatat tcattcctaa aatcatatct
    28501 tcaattgaaa agaattctac atcttctaga tgtggattta gtgttccaac aataaaaaga
    28561 atattgttct ctttacgaag ctccgataaa tagttttcat tcactaaaga gtaatagtca
    28621 cacgtttcta tgacaagatc agtatccctt ggaaagctat cttcaagtat cttttttaat
    28681 ttaaacgaag ttcctaatcc gcttgcacaa gaacagacaa tcatatcctt cttaatattt
    28741 gatgattcat aaatataatc cacattttga ttttctttta cttgttcaag aatctgttta
    28801 agcggtaaat ctaataatat ctgatttgca acatcaatag ccattcttgt tgaaacattg
    28861 tttagtagtc cgaatttttt ttgagcgtct agttccaaat ttagataaat tttctccagt
    28921 gaacccatat caacaagtaa aataatttct tcatatccgg tacactcttg taagtatcta
    28981 ttaattttgt ctgcaatctc actacttgaa gtcattagtg gcatatcaat ggcgtcgtat
    29041 atatatctct caacgatcct attagcagta cttgctatac aggttgccgt tagatctcca
    29101 tgagccaaaa tcagtgctaa acgtttttgc tgaaattctt tactacacat taacttaaat
    29161 aaaataacca aaacattttt ttgaattttt gttaaataaa tatcccctat ttgaataaaa
    29221 cgattgacta attgaacttc tcgactacac caactattaa tatcattgat gaaggaatat
    29281 aatttatcgt ccgatgttaa ttggatttga taaatccaag aattcaaaaa gatagacgtt
    29341 atttgaagat aataatagtc aaattctatt tgaaaatctc tctctatttc cttaacatac
    29401 tgtctaaatc gaatctcttg ataggtggga tatgttaaag ctgaattatt ttggatatca
    29461 acattctttt gtctaaaata tctatcatat ttctcaaaaa gctgctgctt atcagtgtct
    29521 ttcttccacg tttgataaag atactctata agttcgttga tattaaaaac ttcgtctact
    29581 ttttccaaat catccatttg aatatatttt aattcattct gtcttttcat aaccttcatg
    29641 ccgttgaaat tcacccattg tggtaaatga tacatctgaa tataaagttc ttccaaatca
    29701 tctaagaaat aaaaggcatt ggcacaggtc aattgaatga tatttttcaa attgccaaca
    29761 ttttcactaa agcgatagtt taataaaaaa tctaataatt gttgagatat cagtattttc
    29821 tttgctagtc tttcttcttc tgatttaaaa aatatcttga ttaactgttt tctttcataa
    29881 agagaacgac tgtctaatgt tggaagtgtt agctttacag gtattcttct caacaaagtt
    29941 gataatagtt tatcctcagg tggttctgat gttgcaaata taatccagca acttgatgtg
    30001 taccagtttt cattatcacc catccgatga aatttcctcg tatccataaa ctgaaaaagt
    30061 ttctcctgac attcattgct caatgcatga atttcatcta aaaataagac tccattatta
    30121 gctaattctg ctaaaccctt tcttccatct tcggctcctg tatatgcacc ttttacatgc
    30181 ccaaaaaaat tggacaagaa aagttctgga ttattggcat attcactaca attaactgaa
    30241 atgaaagatg ccttctcttc tatataatta ttggccaaca aatccttata cacataattt
    30301 gctaaatagc tttttccagt tccagtctcg ccatgaagta aaatgggtaa gccatttggc
    30361 ggatagtaga ttgctgcttt aatttgagca attacatttc gcagacttcc ttgacttccg
    30421 ataacccttt ctattccaac cctaaaagag ctatcatacc atttttccgc ttcctctgaa
    30481 gagctaaaaa tttgttgatt tggaactttc tccggtaaag aaaagaagat tactggacga
    30541 gtattgattt ttccaaattc tccatctttt acgaattcat tcaaatattg agagacaata
    30601 ttacgactaa catttaattt ttcagatatt ttctgtgctg tattgttccc ccatttattt
    30661 ttaatctcag agttctctat gacataattg acaatgcgtt cttttaattc ttccatcaca
    30721 gcacctcctc gacactattc gaattttgcc gacagtattt ttccataaat atgacttgct
    30781 aaaaaggctt tgtttagatc attcaattgg aacgttgctt gttttgcttg atccgctaag
    30841 acgttgaaaa gatactctgg cgaaatcgca taccccaaaa tatcaacgat tgttgcaatc
    30901 agatcttctt ttgattcttc atctgaaagt aattcacgat acaacatcgt ttgtttgctt
    30961 actgtgtcat attcctctaa ggattcatct gctaaaagaa ccacttgttc caacaaacgg
    31021 tcggataaat atttatagaa aatcagcccc aataaataat ttttatattc tgacgcatcc
    31081 attttactac ggagattatc tgccgcacta aataacttct gatttaattc tgcacccaaa
    31141 aagatacact cctatctctt atttaaagtt gctatatacc ctcttatttt agcatactca
    31201 gttttagaaa aagcaaaaaa ataaagaaac ttattcttga taacaagcta cgtgacgtat
    31261 atccctaaca caaaaatagc catatccttt ggtttggata cggctatttg atattactca
    31321 ttcaattatc aaatcacagt aggtgtgaat ctcatcggga tcggcaatat ctacttctga
    31381 ttaggatcaa aggtgatttt ctcaataaag gtgtgataca aagctttaac ttgcttcttc
    31441 tcactctgga taagaaagca atccaaacta gtcaatatct gttggacatc tttgacttga
    31501 atcggttccc cttgttgttg aaagatccct aagatttcat tctctcgaat ttgtaactgt
    31561 cttcgttttt cagtcaattc atttaaacga tcttttaaca tcggaaacaa ttcgggacta
    31621 tcttccaaag cagcttcctg tttttgctct aatagttaga ttttgcgtac cattttcttc
    31681 ttttattttc cttcgattgc tgattttaat atagtctaaa ggaaatttat atttattgta
    31741 ttctttataa cttcaagaga acttcgtttg agtattactg tattttttta attccataag
    31801 aaaattagtt attacctgcc acctaatcag caaatctgaa cctgagtcaa tataattaat
    31861 attattttcc atctccaaca ttgtaagtac tgttggtgtt aatataggga ctttgtacaa
    31921 ttgtattttt ttgattttat catctgcaca aaggagattt tttaaatcta atccttcttt
    31981 tatttcaaaa gttgaatttt ttaaatctat aagagtaaaa ccaattcctt catactgaat
    32041 ttctgaaaat cgaatttttc tcccagttga tagatcaagg ggcagaaaac cttttattac
    32101 attgtgagtg tcaccatcat ttacaatgtc attaggaatt ttgattttag aattctgtgg
    32161 agcacctatt ggaagataat aattcattat aattttgtag tcttcatgag gaaataaagt
    32221 acttttagga ttgctaagat attctcgtac cgaatctaag aatggcttat ccattgaatc
    32281 ttcatcccca tttaaactaa aaaacattcc tacaattgct tttgcaactc taagtgaatt
    32341 cattttcttt attgaatgtg ttaaatcaga cttaccattc tttaaacttg tattgttata
    32401 aaggaaattt ttattgtctt ctgcaaatct aatatattcc tcaacatact cactaccaaa
    32461 aaaactatta catttactac ataacg
//
LOCUS       DARBFD010000023        32178 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000023 DARBFD010000000
VERSION     DARBFD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.023"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(407..766)
                     /locus_tag="SCK08_001027"
     CDS             complement(407..766)
                     /locus_tag="SCK08_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEG1200935.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="SCK08_001028"
     CDS             complement(796..1137)
                     /locus_tag="SCK08_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200936.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="SCK08_001029"
     CDS             complement(1134..1808)
                     /locus_tag="SCK08_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1200937.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCK08_001030"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCK08_001030"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEG1200938.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="SCK08_001031"
     CDS             complement(4322..5512)
                     /locus_tag="SCK08_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1200939.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="SCK08_001032"
     CDS             complement(5533..6060)
                     /locus_tag="SCK08_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEG1200940.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="SCK08_001033"
     CDS             complement(6107..8896)
                     /locus_tag="SCK08_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1200941.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="SCK08_001034"
     CDS             complement(9043..9243)
                     /locus_tag="SCK08_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1200942.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="SCK08_001035"
     CDS             complement(9696..10016)
                     /locus_tag="SCK08_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200943.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="SCK08_001036"
     CDS             complement(10519..11637)
                     /locus_tag="SCK08_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200944.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="SCK08_001037"
     CDS             12108..13415
                     /locus_tag="SCK08_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEG1200945.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="SCK08_001038"
     CDS             13536..14735
                     /locus_tag="SCK08_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1200946.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="SCK08_001039"
     CDS             complement(14762..16030)
                     /locus_tag="SCK08_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEG1200947.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="SCK08_001040"
     CDS             complement(16200..16841)
                     /locus_tag="SCK08_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEG1200948.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="SCK08_001041"
     CDS             complement(17250..17753)
                     /locus_tag="SCK08_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1200949.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="SCK08_001042"
     CDS             complement(17876..18640)
                     /locus_tag="SCK08_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEG1200950.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="SCK08_001043"
     CDS             18748..19023
                     /locus_tag="SCK08_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEG1200951.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="SCK08_001044"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="SCK08_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1200952.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="SCK08_001045"
     CDS             complement(20579..20980)
                     /locus_tag="SCK08_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1200953.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="SCK08_001046"
     CDS             complement(20994..21803)
                     /locus_tag="SCK08_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1200954.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="SCK08_001047"
     CDS             complement(21790..22680)
                     /locus_tag="SCK08_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1200955.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="SCK08_001048"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="SCK08_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="SCK08_001049"
     CDS             complement(23348..24289)
                     /locus_tag="SCK08_001049"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEG1200956.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SCK08_001050"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SCK08_001050"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1200957.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="SCK08_001051"
     CDS             complement(25274..26470)
                     /locus_tag="SCK08_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1200958.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="SCK08_001052"
     CDS             complement(26488..27216)
                     /locus_tag="SCK08_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1200959.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SCK08_001053"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SCK08_001053"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1200960.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="SCK08_001054"
     CDS             complement(28628..29086)
                     /locus_tag="SCK08_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1200961.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="SCK08_001055"
     CDS             complement(29083..30546)
                     /locus_tag="SCK08_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1200962.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SCK08_001056"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SCK08_001056"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1200963.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="SCK08_001057"
     CDS             31602..>32178
                     /locus_tag="SCK08_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1200964.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6921 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DARBFD010000024        29233 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000024 DARBFD010000000
VERSION     DARBFD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.024"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..837)
                     /locus_tag="SCK08_001058"
     CDS             complement(<1..837)
                     /locus_tag="SCK08_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1200965.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFM"
     gene            complement(1061..3001)
                     /gene="thrS"
                     /locus_tag="SCK08_001059"
     CDS             complement(1061..3001)
                     /gene="thrS"
                     /locus_tag="SCK08_001059"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEG1200966.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3338..3517)
                     /locus_tag="SCK08_001060"
     CDS             complement(3338..3517)
                     /locus_tag="SCK08_001060"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEG1200967.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3564..5387)
                     /gene="cadA"
                     /locus_tag="SCK08_001061"
     CDS             complement(3564..5387)
                     /gene="cadA"
                     /locus_tag="SCK08_001061"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1200968.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5665..7944
                     /locus_tag="SCK08_001062"
     CDS             5665..7944
                     /locus_tag="SCK08_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1200969.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8037..8684)
                     /locus_tag="SCK08_001063"
     CDS             complement(8037..8684)
                     /locus_tag="SCK08_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200970.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8863..9582)
                     /locus_tag="SCK08_001064"
     CDS             complement(8863..9582)
                     /locus_tag="SCK08_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1200971.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9713..10864)
                     /locus_tag="SCK08_001065"
     CDS             complement(9713..10864)
                     /locus_tag="SCK08_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEG1200972.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11081..11821)
                     /locus_tag="SCK08_001066"
     CDS             complement(11081..11821)
                     /locus_tag="SCK08_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1200973.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11825..12148)
                     /gene="rsfS"
                     /locus_tag="SCK08_001067"
     CDS             complement(11825..12148)
                     /gene="rsfS"
                     /locus_tag="SCK08_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEG1200974.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12205..12798)
                     /gene="yqeK"
                     /locus_tag="SCK08_001068"
     CDS             complement(12205..12798)
                     /gene="yqeK"
                     /locus_tag="SCK08_001068"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEG1200975.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12782..13432)
                     /locus_tag="SCK08_001069"
     CDS             complement(12782..13432)
                     /locus_tag="SCK08_001069"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEG1200976.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13442..13753)
                     /gene="yhbY"
                     /locus_tag="SCK08_001070"
     CDS             complement(13442..13753)
                     /gene="yhbY"
                     /locus_tag="SCK08_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEG1200977.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13769..14878)
                     /gene="yqeH"
                     /locus_tag="SCK08_001071"
     CDS             complement(13769..14878)
                     /gene="yqeH"
                     /locus_tag="SCK08_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEG1200978.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14875..15402)
                     /locus_tag="SCK08_001072"
     CDS             complement(14875..15402)
                     /locus_tag="SCK08_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEG1200979.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15582..16367)
                     /locus_tag="SCK08_001073"
     CDS             complement(15582..16367)
                     /locus_tag="SCK08_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEG1200980.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16360..17229)
                     /locus_tag="SCK08_001074"
     CDS             complement(16360..17229)
                     /locus_tag="SCK08_001074"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEG1200981.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17230..18609)
                     /locus_tag="SCK08_001075"
     CDS             complement(17230..18609)
                     /locus_tag="SCK08_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEG1200982.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18617..19036)
                     /gene="fabZ"
                     /locus_tag="SCK08_001076"
     CDS             complement(18617..19036)
                     /gene="fabZ"
                     /locus_tag="SCK08_001076"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1200983.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19061..19537)
                     /gene="accB"
                     /locus_tag="SCK08_001077"
     CDS             complement(19061..19537)
                     /gene="accB"
                     /locus_tag="SCK08_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEG1200984.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19540..20775)
                     /gene="fabF"
                     /locus_tag="SCK08_001078"
     CDS             complement(19540..20775)
                     /gene="fabF"
                     /locus_tag="SCK08_001078"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEG1200985.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20794..21531)
                     /gene="fabG"
                     /locus_tag="SCK08_001079"
     CDS             complement(20794..21531)
                     /gene="fabG"
                     /locus_tag="SCK08_001079"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEG1200986.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21543..22460)
                     /gene="fabD"
                     /locus_tag="SCK08_001080"
     CDS             complement(21543..22460)
                     /gene="fabD"
                     /locus_tag="SCK08_001080"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1200987.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22567..22791)
                     /locus_tag="SCK08_001081"
     CDS             complement(22567..22791)
                     /locus_tag="SCK08_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1200988.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLGLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22844..23806)
                     /locus_tag="SCK08_001082"
     CDS             complement(22844..23806)
                     /locus_tag="SCK08_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEG1200989.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23816..24256)
                     /locus_tag="SCK08_001083"
     CDS             complement(23816..24256)
                     /locus_tag="SCK08_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1200990.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24562..25527
                     /locus_tag="SCK08_001084"
     CDS             24562..25527
                     /locus_tag="SCK08_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1200991.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25584..25814)
                     /locus_tag="SCK08_001085"
     CDS             complement(25584..25814)
                     /locus_tag="SCK08_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEG1200992.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25900..26787)
                     /locus_tag="SCK08_001086"
     CDS             complement(25900..26787)
                     /locus_tag="SCK08_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEG1200993.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26876..28099)
                     /locus_tag="SCK08_001087"
     CDS             complement(26876..28099)
                     /locus_tag="SCK08_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1200994.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28116..29162)
                     /locus_tag="SCK08_001088"
     CDS             complement(28116..29162)
                     /locus_tag="SCK08_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1200995.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8093 a         6413 c         5043 g         9684 t
ORIGIN      
        1 cataaaatat tcttggattg cttctcgtgt ttttttcact ttttcgctaa gcttttcgtt
       61 tttagccata tagaaataaa aaatgatatc gatcgtagca aattgggcca aaagtgaatt
      121 agttgccgca cttcgattcg tgatttcttt tggtctagat gtttgtacga gtacatcggc
      181 tatcttgcta agcgggttat ttccaaattg cgtcaacgca atgatttcga tattcatgct
      241 tttcgccagc tcggctaatg cgactacatc tgcacttcta cctgagtttg aaacaagcca
      301 aagcactttc tttttctcat tgctgacaag ttgcgggagc aagacgtgga tatctttttc
      361 aaaaatgatt ggcttaccga cacgagacca tttttgcaga atatcttcta ctactaaaga
      421 agaggcgcca accccatata aaaaaacagt ctctgccgat tccaaacact ctacgacttt
      481 ttggatcgtt tcttcttcta gaatatctac tgtttctttt atcgctactt gtgcattgtt
      541 accaagtttg tttttgatag agtaaaagga ctcatttgct tctatatcac taaaaccttc
      601 atcgattcca gtctgtagtt ctgttgaaat cgtgattttg aagtccgtca gactttgaaa
      661 accgattttt ttggaaaaac gtacaactgt cggcgcactc aacccggaag cttctgctaa
      721 ttcattagct gtcattgtag ggaccttctc aatattaccg ataatgtagt tggccaactt
      781 tttttctgct gaagaataat cttccatcat actttcaata cgactaatta cgctcataag
      841 ttcctccacc cttttaatga tagtctatgt aagtatacca tatcagaccc ttattcacgg
      901 tcgtatactg gataaaaaac aaaaaaacaa tccgcccaac agaatatctt atcccatcaa
      961 tcccacagat tctattgagc atctgtgtct ttgaggaata cctgttatct attgttcgga
     1021 ttgctttcta tctactactt ttttacagga aatttaccta ttagttttct ctgctgtaat
     1081 ttttgacttc tgcttccata gcatcaataa agatattcaa atcttctaca gctgtttctt
     1141 tacttccata acgacgtacg ttcactgttg catcttccaa ttccttgtct ccaacaacta
     1201 attggtaagg gatcttttgt gtttgagagg cacgtatctt atagcccatt ttttcattac
     1261 gatcatcaac ttctacacgc aaccccttca tttgcaaacg ttctttgatt tcataagcat
     1321 aatcgccatg tgcatcaaca gaaacaggga tgattgttgc ttgtataggt gccaaccatg
     1381 ttggaaaagc gcctttgtat acttctgtta aataagcgac aaaacgttcc attgtagaaa
     1441 caattccgcg gtggataact actggacgat gcgtattttc cccgtcttct ccaacatatg
     1501 tcaaatcaaa gcgctctggc aataagaagt ctaattgtat agttgataat gtttcttctg
     1561 taccaagagc tgttttgact tgaacatcta gttttggtcc gtaaaaggct gcctcgcctt
     1621 ctgcttcaaa gtaatctagt tctaattcat ccatagcggc tttcagcatt gcttgggctt
     1681 tttcccacat tgcgtcatca tcaaaatatt tatctgtatt gtttggatcg cgatagctca
     1741 agcggaaacg ataatctgta atattgaagt cagcatatac tgccaccatc aattcaagcg
     1801 tacgtttaaa ttcatctttg atttgatcag gacgaacaaa tgtatgacca tcattcaacg
     1861 tcatttctcg tacacgttgt aaacctgaca aagcacctga tttttcatag cgatgcatca
     1921 ttcctaattc agcgatacga attggtaatt cacgataaga atggatcgtg tttttgtaga
     1981 ccatcatgtg atgagggcag ttcattgggc gaagaacaag catttcgcca tctcccatat
     2041 ccattggcgg gaacatgtct tcttgataat gatcccagtg tccagatgtt ttgtacaact
     2101 caacatctcc cattattggt gtatatacat gttggtaacc tagactaact tctttgtcta
     2161 cgatataacg ttcgatcgtg cgtcggattg ttgccccttt tggaagccag aatggcaaac
     2221 cagaacctac ttctggggaa accatgaaca agtcaagttc ttttcctagt ttacgatggt
     2281 ctctttcttt tgcttcttca cggagacgga tatattcttt caaagctttc ttgtcaaaaa
     2341 aggctgtccc gtatacacgt tgcatcattt gattgtttga gtttccacgc caataagcac
     2401 ctgccactga taataattta aatacttgta tacggccagt ggatggaaca tgtggtccac
     2461 gacacaaatc gatgaattcg ccttgttgat aagcagtaat cacttcttct tctggcaatt
     2521 cactaatcag ttctactttg tacggatcac ttgcaaaaat ttctaatgct tcctgttttg
     2581 aaagaacacg gcgttcgatc ggcagatttt ctttgacgat tttcatcatt tctgcttcga
     2641 ttgccggcaa atcttctgca gttacttggt tttgtccatt atctgtatcg tagtaaaacc
     2701 ctgaatcgat agctggtcca acgccaaagt ggatatttgg atagaggcga cgcatggctt
     2761 gggccatcaa atgagctgct gaatgacgga ctaagccaag tgcatcttca tggtctggtg
     2821 tgatgatctc aatacttgca tcttcctcga ttggacgagt caaatcaacg agttttccgt
     2881 tcactttacc agctaaagct ttttttgcta agctttttga aatgctttca gcaattgcca
     2941 atgttgttgt tccagactca aattctttga ctgcaccatc tggaaaagta atttttatca
     3001 taatagtttc ctccttgaaa tgttttgatt gatcagatca tcaagctgaa aaacaaaaaa
     3061 atcctagcat ctcttgaaaa gagatactag gacgaaaagt tcgtggttcc acctaatgtt
     3121 caaacgcaaa tgcgttcctc aagcgttgta acggtccgac cgcccttgct tcaacaaggg
     3181 tttcgaaagt ggtcatcctt tggttttctc ctggaaaagt ttcagcctag cttttcctct
     3241 cttatcgaag actctcaaat tcagttggct ttctcatcaa tcaattatgt gtttatttaa
     3301 ccactaataa atagaaaaat caagtgggaa actaaactta ttttttccat tccccattta
     3361 caccgtatgt tccttttttc atctcattga tgatgacatg gatgtgttct tttggtgcct
     3421 gcgtgttttt atgaacagct tccgtgatat ctttcatcat attttctaat tgttccggac
     3481 tgcgaccttc tactaattcg acatgtacga atggcataat gctctctcct ctttctgatc
     3541 gttctttttt caactcgatt ctatcatact atgtttgtct tgggtactga atcaggaaaa
     3601 atggctggat ctttttttgc tctcaaggca ctcaaaatag agaccgtatc cacgacttct
     3661 tgcaaaacag ctccgaataa tgcggggatt agtcccgtac ttgcaattaa catcaaaaaa
     3721 atacaaataa agatgccaat cagtacagac tgtttcgcta ttctcatcgt atctcttgaa
     3781 atcactactg cttttgctac acgcaagaga tcatctttca agatgaccgc atctgcactt
     3841 tcactagcag ctgtagaacc atgagccccc attgcaatac cgatatctgc agcagcaagt
     3901 gctggtgcgt cattcacgcc gtctcctacc atcgcaacag gtctttgatc tttagggata
     3961 ctttctaaaa tggtcagttt atcttgaggc aagctacggg catgtacaga atctaattgg
     4021 actttttctg caatcgtttg agcaatggca gatccatccc cggtgatcat catcaaattt
     4081 ttcacatcaa gttttcttag ctccttgatt gtctttgctg cttctgggcg aatctgatcc
     4141 gaaaatagga tcgttcctgc aaaagatcga tcaattgtta catagattgc tgtattggac
     4201 gtttctattt cttcagctga aacaaaagag gcacgtccaa tctttacttc atgattattg
     4261 actactgctt ggattccttg accggtcact tctttcagtt gactaacagg atacagttgg
     4321 ctgtttttca cgttgttcac taacgaacga gctaagatat ggctagattc ctgttctgca
     4381 ctagctgcat acagaagtag ctcttctttt tttattccat tgactggctg aatcgcctct
     4441 acttccaata tccctttggt aagcgttcct gttttatcaa aagcaatcgt ttttgtacgt
     4501 gcgagctttt caatcgttgt tccagttttt acaacgatcc cgtttttact tgaacggctc
     4561 attcctgcaa ctaaagcaat gggcgcagct agaatcagtg gacaaggtga tgccactacc
     4621 agtacctcag caaatcgtaa agggtctttt gagaaccacc aagaaatacc accgatcaaa
     4681 taagcaatca acgtgaaagg gactgcataa cgatcagcta aacggacaaa tcgggcaggc
     4741 ttttcttttg attcttcgac tagtttaata atcatttgat actggctgtc tgcggcacgt
     4801 ttttcagcga tcatttggat cgatccgtct ccatttaaag accctgacat aatctgatct
     4861 ccaggtttct tggtttccgg gcgggactca cctgtcaaag aagattcatc gaccgaagat
     4921 tctcctttgg tcactcgacc atctgcgggt acaagttcgc caggcttgac tagtaactgg
     4981 tcatcaatat ttatttcatc gattactata tcctgcaatt ttccttcttt gaatcgatgg
     5041 gcaattcttg gggtgttatc taacaaagac tgcagttctt tacccgctcg tttattcgcg
     5101 taatcctcta gactatctcc gcccgtaagc atgatcaata tcatcaaact agcccagtat
     5161 tctttgacgg ctaatgttgc aaggattgcg gtaatcgcta atacatctac gccatatctt
     5221 cctgaacgta acgtcttgat catttccaaa aacatcaaga aggcgagaat ccctccagta
     5281 aatgcgacaa tcgcaaatgc aagggccggc tggtgaaaaa caaattcact taataaagct
     5341 atcccaccaa taagcaaagt aagtaaaaat tttttgaaat gtgtcattgt tttttctcct
     5401 cttttatgtg acttagagag gttagtaata aatttccagc tgcctctttc atcttaagca
     5461 tagtccttga ctagcacttt ttaaaacaat aggccttaat tgaaaacaac tatcactatc
     5521 agcgtttcca aaattcatca gttctacaaa caaaaatatt cagcaaggat tactttttcg
     5581 attcatcagt taaaaagtac cttttttcga aaaactatgc tatactttta attcgttatt
     5641 tacggcggaa ggagcaaaca tgcgatgaat gagaaacaac tagaacaaac gcacgtggat
     5701 gaaacgatcg aactaatcca gatcgaacaa aatatgctgg atacaaaaca acaagaattg
     5761 actcaggaaa tgaatcattc gacaaaagac acagctaatc acaagattcg agctggttcc
     5821 aatgaagctt tttatgaatc tgctgttgaa tatcgccaac atgagcagga attgcttttg
     5881 aaataccata ctcttgaagc acagcaaaaa cgcctgcaaa cgttgtctgt catgaaagat
     5941 agtccttatt ttgcccgtat cgactttaaa gaagaccgtg aaacagaaac attatatcta
     6001 ggtatcgctt ctttaagaga tctggcagaa gaaacaatcg ttattgactg gcgcgcacca
     6061 attgccaatc tttattatga aggtgagatt ggtcccgcat tttatgaaac tgaaacagat
     6121 agaatagatg tcgatctgca gttgaaacga caatttaaaa tccaagaagg acgtatttta
     6181 tctatggtag atacgtcgga agtaatcaat gatgactttt tgctagagat attggatgaa
     6241 tcatcaagca accatatgaa aaacatcgtc tctacgatac aaaaagcaca aaatgcaatc
     6301 attcgggaca caacaagtaa agtaatgctg attgaaggaa tcgccggaag tggaaagaca
     6361 tccgctttgc ttcaacgtat tgcttttctg ttgtatcata atcgcaagtg gctcgaggct
     6421 gaaaacgttc ttctattttc acccaaccat ttgttttctg attacatctc gactgtattg
     6481 ccttcattag gtgaaagcgg cgtgcctaca cagacattca aaaattattt ggtgcagctg
     6541 ctgccagagt tttctttact agatgaacag caacaagaaa gtggtttttt gctaggggaa
     6601 aaagatccga tacaagtgat gaaaagcgga ttgaccttag tcgatcagat tgatcgctat
     6661 atccaaggca tcacaaacta cggtcccttg ttccgtgata tgaaaataaa tggccgtacg
     6721 atcctatcaa aggaatcgat tcgccaatgg tataaagaaa caaatgaaca attaccttta
     6781 catcatagac tttctctatt acaaacgaag ctcctgaaaa aattaggcgg tctgcaaaaa
     6841 gacgaaacac gtcagcaatg ggtcaaagat ctggcggaag aacagctcca agaactatac
     6901 gcaacagatc caaatcttga atatacagaa caaaaggagc gttcattacg taaaaaactg
     6961 gccaaccaaa tcgtacgtaa aagattccgt cgaatccatc gagggatcat gaattatcaa
     7021 tttgtcaata taacgaaaca atatctccac tttctacaaa ctgtgccact ttctcttcta
     7081 acacgacata aaatcagcga gaccaggtgg caggaacatt tgacaactgt tcgtacacac
     7141 ttgcgtcaaa gagaattgcg tcaagaggat gctgtactat tctttttact gatgcgccga
     7201 acatatccag tgacggttac acacaaagct cgctatatct ttattgatga aatgcaggat
     7261 ttcgcaccat cacaagtggc attgctgcaa gccttgttcc caaaagcaaa tttgactttc
     7321 tgtggagatc taaatcaaaa tgtctttgga aatgaaacga tcgttggatc attggaagac
     7381 cttttcgtgg accgtgaagt cactcgtttc caattaacga caagctatcg ctccacaaaa
     7441 gaaatcactg attttgctaa tcattttatt gtgaaagaac atcaagtaga aacaacagca
     7501 agaaaaggag accttccttt gctcatccaa ggaacaacag atgatatcaa attgaattgg
     7561 ttagcagata ccattcgaac gactcaggga aaagcccggt attggcgtac agcaatcata
     7621 gctaaaacac aagaagaatg cgaagctctt tatgctcaac tgccagacga gttgaaacaa
     7681 caagtccagc tgattttaaa tgagagtgac tttatgaagc gttccattat tttacttcca
     7741 gcctttttag caaaaggtct ggagtttgac cgagttttct tgtgggatgt agatgcagaa
     7801 catttttctt ctcagcaaga caaattgatc ctttacacca tgtgtacacg tgcgatgcat
     7861 gaattgaccc tttttacctc caataatgaa agtcctttgt taaacaatat ggatatttca
     7921 tcttatgaga aaaaagtact gtaagtaaaa agacaactgc tatcattaat gttagcagtt
     7981 gtctttttat ttacaataat aattgcagaa aaagtaattc aatcatttaa ggtcatttat
     8041 tctatatata aatccaaatc tgtttcagga tattttaatt tgacaagcga aataatcatt
     8101 ttcagttctt ccatgttttc tggatattct gggtcaaata ccttgctatg acggccaatc
     8161 ccgccattgt cgatcaatcg gtaaattcga ttccattcaa tgaattggtc aattgattgg
     8221 aaattgctga ttggatagcc gccacaatcc aaaatctctg atacacgaaa tgctaggtat
     8281 gcactatcga tcactttatt ttttttcgtg tctatcttct catttctttc gataagaatt
     8341 ttttcaggac gatgtttcaa gctccactct aatagtagaa agaaagcctc catccgatac
     8401 tcttcttcaa ataaccgaaa ggctgaatca gaacgacgaa aaagcagcgg aatcagataa
     8461 ttttgtctat aaatcagcat tggtgcgcgg tccaccatac actctaaata acggttcaga
     8521 taccctgtac cgacatagtt tcctaatcga tagccttcaa cgccgaatct tgcgtatttt
     8581 tgcgctaatt ttgaacaatc agttacgttc acagctaact cttctacttt gcggtaaccg
     8641 cttaagatac gttcaaaagg ttctaaagtg atttttgctt ccatattctt ccccgtccat
     8701 cttccacata cttcatatca tttcacatga tacttatgct ctcatcatac cacaggttga
     8761 attagaaaag aaaataaatc acacaaaaaa gcaagacaat ctgttagtga gattgtcttg
     8821 cttttcgaaa aagactgaat acttttcgtt aaaactggtt gcttagtctt ctaatgccac
     8881 attatggaac acatgctgta catcttctag atcttccagt gcatcaatca ttttttcaaa
     8941 tgtttcttga tcttctggag atagtgttac ctcgttttga gggatcattt ggatctcagc
     9001 gattgaaaaa tcagtgattc ctttttcttt taatgcttct tgtatactat ggaaatccgc
     9061 tacttcaccg taaacgatga tttgtccttc ttcttccatt acatcgcgaa catcgatgtc
     9121 tttttccatt aggtattcaa ggatctcgtc agcgtcttct cctgcaaaag caaagattgc
     9181 tgtattgtca aacatgtaag aaaccgctcc ggaaacaccc atgttcccgc catttttccc
     9241 aaatgctgcc cgtacgtctg ctgctgttcg attcacattg ttcgtcaatg tatcaacaat
     9301 caccattgag ccgcttggtc caaatccttc atagcgcaat tcagagtatg tttcatctcc
     9361 tgcacctttt gctttttcga ttgctcggtc aataatatgt tttggtacat tataagtttt
     9421 agcgcgatca atgacgaaac gtagtttttg gtttgcatgc ggatcaggat cacctgattt
     9481 tgctgccgcg tagatttcaa tcccaaattt tgcataaatc cggctattat tagcatcttt
     9541 agcagttttc tttgctacga tatttgccca tttacgtccc atattttcac ttcgctttct
     9601 gatttgatag tttcataacg caaaaacgtc ctgttccatt atacttgtga atcacgattc
     9661 tgtgaagctt tttttctttc ttccttcatt cttctcattt ttagtcaatt atctatactc
     9721 ggattgggat ttgcccgaaa ttctgttccg gaatattgcc tcccaattga tagatttgat
     9781 cacttctaac taacagttcc gcttcttgtg cctctttttt tccgattcgg gaataaatag
     9841 aaatctcttt tttttctttt aagaatcttc ttccagcatc ggtaaatcca agtagtcgaa
     9901 gataattttt attttgttgg acaggaatct cttgcttttt gatattcagc aagacataac
     9961 aagataatcg ttggatccgt gtccatgtat atctttttgt ttttaccttt gacacaaaat
    10021 cctcaaaact atcagcagtt gcagctgctg ttttcatgcg cgattctatt ccttcggtca
    10081 tctgatagat atctgtcagt tccagcaaag tagaagataa caaccgataa cgcaaatacg
    10141 gccaataaga ttcccaagtc tgcagcgaac aagttgaaag atcttcttct gtctgaggcg
    10201 gcacaagtcc agctattctt tttccttctt ctaacgcatg gcgaatggct gttgcactgg
    10261 ctatcctctg atctgtgatg attttttcat gatagcctgc acttttacgt cggatcggat
    10321 gtaattccat agggtggaca tacttggcat tttcctttgc ataacttaat cccaagatat
    10381 gatttggcga agaaaaatca agcgtaatat cgggatacag actgcgaaaa acctcagtca
    10441 ttttttgcgg ataactatac gtttgatcag acagttcttg aaatgcctct tctatcctat
    10501 cttgattctc ttggacaaag cgtccgaaac tgctgtaatc aaacgaagtc tcttcttctg
    10561 ttccaaaaca caaactgtca cagtccagtg tttgcaagat ttttactgca cctttagcaa
    10621 aatagtcagc tgactgaacc gcccattcga cgggaagctc cactactaaa tccacaccat
    10681 tttgtaaagc ttcattcgcg cgatgccatt tatcgatgat agccggttcg cctctttgca
    10741 aaaaattccc gctcattaca gcaatgacga cctctgctcc tgttattttt cgtgccattt
    10801 ccacatgata gcgatgtcca ttgtgaaaag gattgtactc tacgataatc ccgcagcttt
    10861 tcatgcgcat cctccttcat agttatcgat aaaaaagaaa tagagacaag taaaaaaagg
    10921 caaaaatcgg aaaaaatacc cgaactatat aaactattct cgcaaacaca atacttctgc
    10981 gacgaaaagc tgcactgcaa gagcaatgcc tatatcgttc ggatatataa tttatgttga
    11041 agatttattc ttttcttgtt aactcgagca ggcaaaaccg ctagtctttg tatgctacaa
    11101 agaaccagcg ctttgatttc tctgatgctg cttcgtctgt aaaatcagaa aatgcttcga
    11161 ctttcgaaaa gccgctgttt tccagcattc ttaaataact ttccaatgga taggtacgct
    11221 cttgatgtag ttcatcttca cgaataaatc gaccagaccc ttcttgttct tcaacgaaaa
    11281 aggtaagaaa atgttcgatg ctgtgtttct cgcttcctgg ataactatcc cacaaaaaag
    11341 caaaattatc cccttgataa tgatagctat attctggaaa aacctcatct acttgatagg
    11401 tggagtgtac gtcaaataaa aatcggccat tttcttctaa cgcttgatag acttcatcaa
    11461 acacttgctg tacctcttgt ctatttttca tatagcacag cgaatcagaa aaacaggtaa
    11521 ttgcctgata ttgtccgatt tctgataaat cgagcatatt gccttccaca aattggatcg
    11581 atgcgtcttc ttcatatgct cgctggctgg caatcatcaa catctcttct gaaaggtcta
    11641 accctgtcac tttatatcca tcttttgcaa aggcaaccgc taatgctcca gtaccgcatg
    11701 ccagttccaa aatatttttt gtgtcttctt gtaaatgacg tttcgaaaaa gctaaccatt
    11761 gctgataaag tgtttcgtcc atcacttcat cgtaaatgaa agcaaatgtt tcataagcca
    11821 tatgttaatc caaccattca ctaagatcga ctagcggagc atctgaccat agtttttcca
    11881 gattatagaa atttcgttct ggcgcatgga aaacatgtac gatcacatct ccaagatcga
    11941 tcaataccca ttttccgcct tcttttcctt caatacgttt tacttcatat ttgttttctt
    12001 cttctttatc aatgatttct tctgtaattg cgttgatttg acgttcgcta tttgctgaac
    12061 agatcatgaa ataatctgcc aacaaggaaa catttcgaac gtctaaagcg ataatgtctt
    12121 ctgcccgttt tgaatctgct gcttcaatcg caatttttaa ttgttcttta ctatctatga
    12181 tgatttcctc ctaatatgag cagcttattt ttttgctacc cagtaattat atgtttcgat
    12241 tgtttttgga taaatctgtt gttcttgcgc aagcaaatgg gccaatgtat gttttgtttc
    12301 aaatgctaca gcttcgtcta gatcagccca tgcaatggct cgtgcatctt gtaccccagg
    12361 aaaatctcga cctggttcaa tatagtctgc tacataaatg attttgtcaa ggagactcat
    12421 cttagcagcc ccagtcgtat gcacccggat tgcatgaaga atctctgcat cggtgattcc
    12481 taattcccgc tctacaaacg aagcgccaac aagaccatgc cagattgcat tattataatt
    12541 caataactcc ggatcaaaac cgtctcttac gattaccatt ttgaactcct catctggtcg
    12601 ttcttttgca taatcatgtg tcaatgcagc aatactagct ttttcaggag aagcaccgta
    12661 ttttttggca agagccacgg ctgtttcttc tactccaagc acatgcttga agcgttgttc
    12721 gctcattctc atctgtattt tttgcatcag gttttctcta gaataagcag tatattcatt
    12781 actatagtcc atattcatac aacccttcat tttgaatata atcaattact ttatctggaa
    12841 caaggtagcg gatagaacag ccttctttga tcttttgacg tattttcgtg gaactgatgt
    12901 cgatctcagg tacatctacc caaattacag gatatggagt atccgttgtg tatcctggtc
    12961 gacgtatacc cacaaagttg accatagaag tcagctcatc gattttatac cattttggca
    13021 aatattctac catatcccca ccgataataa agtaataatc tgtatccgga ttgttttgcg
    13081 tcaattcctt catagtatca taggtgtaac ttttcccacc acgagctaat tcaatcgttt
    13141 caatctgcaa aaaaggattg tcttccaccg ctaattccaa catgttgagt cgatgttttg
    13201 catcaatcgt ctgtttctca tcaacatgag gtggctgata agaaggcatc aaaaatacgc
    13261 ggtcaagacc taaattccgg ccagcttgtt cagccatcac taaatgtgca aggtgaacgg
    13321 gattgaatgt tccgcccaag atccctactt gctttttctt ctctaagaat tgaggcattt
    13381 cttcaggaaa taattgtgaa cgaacaaatg tcttagcttg cgtattcatc atggtcactc
    13441 cttatattgc ttttacttct tttgatagcc tttgatactt ttcttttgtg gaaggtttgt
    13501 ataatacaat aacacgtcca atgatttgga cgatttcgca atctatttct ttttctaata
    13561 cttgagctgc ttcttctgca atctcatctg tattttgaag taacgatact ttgatcagtt
    13621 ctcttttttc taaagcttct tcaatttgaa cgatcattgc tgaattgatc ccaccttttc
    13681 cgatttgaaa aatcggctga agatgatgcg ctttgctacg taaaaaacgc ttttgttttc
    13741 ctcttaaact cataattttc cttcttcctt aaatcaatgc tttgcgtttc aatacatcta
    13801 caccctttgg cgcccagccc gcaattacta ctggcgctgt tacagtgatc cagcctagac
    13861 cggcaaaaac gatgtctgtt ttttctttca cagaaaactc aaaacgaaca agctccggaa
    13921 atgcctcgac ttcatcagga cgaggaggct gtaataatcc acctacatgt ttttgataaa
    13981 attcgtctgc tttttccagt ttcgttcgat gaatatttac atcatttgac acataagcaa
    14041 taaatgaagc acgattgccg ctgataaaat caaatcttgc aagtccgccc aagaaaagtg
    14101 tttgcccttc attcagctga taaactttag gtttgatttc ttttaacggt gcaatgatcc
    14161 gcaaatcttt tttacctaag tagtgcgcca tttgatggcg atggataatt cccggtgtat
    14221 caatcaaaaa atgcccgtca tctaaaggaa tctcgatttt atccaaggtc gttcctggaa
    14281 actgcgaagt tgtgattaca tcttgtactc ccgctgtatt tttgatgatt tgattgataa
    14341 gcgtcgattt tccgacattt gttactccga cgatatagac atcccgtcca tcgcggtatt
    14401 cttcgatttt ttctaatagt tccgtcatct catgagattt cttagcacta gttaataata
    14461 catctactgg acgaattcct gcttcgtaag cacgttcctt catccattgt gttaatttag
    14521 tttttttcaa tgatttaggt aatatatcca ctttgttccc aactaaaagt actggattgt
    14581 caccgacaaa acggtgtagt cctgggatta gtgaaccatt gaagtcaaaa atatccacca
    14641 cattgacgat caatgcatct ttttgcccta attcattcag taagcgcaaa aagtcgtcat
    14701 ccgtcaatga gacatcttgg gtctcattat aatggcgtaa tctgaaacaa cgctgacaat
    14761 agacttcccc ggaagctaaa cctttttcca aagccgtttg cggcgtgtat cctaattcac
    14821 cgggtttgtt tgtttgtata acggcaccac agccgataca ttgtaattct tctgtcattc
    14881 gattccacct cgccagccca tatcagggtg ttttgctaat agataggcca taattctttt
    14941 ttcccaaaaa cgattaaatt gtgtcttcca actatctgtt gcgataatgg gttttacaag
    15001 gatgctacgt atccctgcac gattggctcc acgtatatca gtcataatct gatcgcctac
    15061 cattactatc tcatcatctg ctaaatccaa ttgtttcttt gctatgttga tccctcttgc
    15121 taaaggtttc attgctcgtg caacatacaa aagatcaaat ttttcaatcg ctcgcgcgac
    15181 acgttttgaa ttattgtttg aaacaacaac aacagggatt cctgcatttt tcatttctaa
    15241 aatccaagta agcaactctt ctgttccatc tggattattc catgcaatca acgtattgtc
    15301 taaatcggtc aatactgctt tgatgcctag attctttaat tgggcaggtg taatctgata
    15361 gatggcttct accatccatg ttggtttgta atttgaaaac atgctgtctc ctcatctgca
    15421 aaaaatgaag gagcacataa agtactcctc aaccccgtat tatactgcat atcagcaaaa
    15481 aaaactacct ctatactcgt tcgtctttca gaaaaagcat ttctttacaa aatgaccaaa
    15541 aataaagatt gatcacagaa aaatttagaa acaaaaaaca actaatattg gcggaacctc
    15601 ttgtatcgtt tttccagtaa tttatgctga tctaatcgaa caagttccgt caatctttca
    15661 atcaacgctt ttttcataat gtgtgcgact ttatatcgtg gcaactgctc gccgttgaac
    15721 gtttcaggaa taactcgatc aacgattcct aactcgcgta gttctggcgc agtaattttc
    15781 atcaactcag ctgcttctgc ggcgcgacta ccgtctttcc acaaaatcga cgcaaatcct
    15841 tctggagaaa gcacagcata aatcgtttct tccagcatcc agacttcatc cgctaaacct
    15901 agagcaaggg caccgccact tccaccttca ccaatgatga tggcgatgat cggtaccttt
    15961 aaatcagaca tttcgagcag atttctggca atagcctctc cttcgccgcg ctcttctgct
    16021 cctacaccgc aatatgcacc tgctgtattg atcaaggtaa tcaccggtct attgaacttt
    16081 tccgcttgtt tcatcagtcg taaagctttt cgatagcctt caggatgggg agaaccaaaa
    16141 tttctgcgga tattttcctg cagatcccgt cctttttgaa taccgatcac tgttaccggt
    16201 ttgccatcta atcgagcgat tccaccaaca atcgcttcgt catctgcaaa taaacgatca
    16261 ccatggcatt cctcgaaaga atcgaagatc tcttcaaaaa aatccaagct tgtccatcgt
    16321 tctttagctc ttgctaactg aacgacatca tgtgctgtct tatccacgat ctatccatcc
    16381 tttctgtgta tgcaattcaa gaagcgtatg gattttctgt ttcaattcca tgcggggaac
    16441 aatctgatcg acaaaaccat gagataataa gaattcagcc ttttggaaat cttctggaag
    16501 ttcctgcttg atcgtctgtt cgatgacgcg tcttccagca aaaccgatca agctttgggg
    16561 ttctgctaga ataatatcgc cttccattgc aaaactggca gttactcctc cggtcgtcgg
    16621 gtccgttaaa acagttagat aaaaaagacc ttcattgcta tgccgtttga cagcagcaga
    16681 gatttttgcc atctgcatta gagaaaagat tccttcttgc atccgtgcgc cacctgaagc
    16741 tgtgaacaag acgactggaa gactttcctt tgttgctcgt tcgaataatc tggtgatctt
    16801 ttctccgaca atcgttccca tacttcccat gataaaatta ggatccatca ctccaataat
    16861 gaagggaatc ccctgaatgg ttgctttacc agtgaggact gcttcatgaa gtcctgtttt
    16921 atcttgcatt tttttaattt tttccgtata ttcgggaaat gctagcggat ctttggttac
    16981 aagatccgta tcccattctt caaaactttt ttcatcaata atcaaagcta gacgctgcca
    17041 tgcaccgatt cgaaaagcgt atccgcaatg agggcatact ttttcttcac cgatgtcttt
    17101 cgtataaagg atatgtttgc aatttggaca ctttgcccac atgttatcag gaacagatgg
    17161 cgagttagca ggtttgctct ctctattagg attgatccga atatatttct ttttcttaaa
    17221 cagagccatc tatgcgcctc ctatctccgt ttctggtgtc caattcggta aaaattcttc
    17281 ttgtaaaaat gctgtgctgt aatcacctgc tatgactgca ggatgactaa tcaaatccat
    17341 ctgaaattct gcgttcgtga tgattccttc tgtgataaat tcacttaagg ctcgctgcat
    17401 cttcattaat gcttcaaaac gcgtatttcc atggacgatt atttttgcaa tcattgaatc
    17461 ataatatggc gggatcgtat atcccgggta aactgcactg tctactcgca gtcccatccc
    17521 acctgctgga agcagtaagt tttggatctt ccctggagaa ggagcaaaat ggaaagcagg
    17581 attttctgca ttaatccggc attcgattgc gtgtccttgg aatactactt cttcttgaca
    17641 cacattcaaa ggctcaccgg ctgctatctc cacctgtttt tttactaaat cgattcctgt
    17701 gaccatttct gttactggat gttccacttg gattcgagta ttcatttcca tgaaataaaa
    17761 atcgccttct tcatccatca gaaactcgat cgttccggca ttttcataat tgactgcttt
    17821 agctgctcga acagcagcgt ctcctagtgc ttctcttttt gtttgtgaga tcactacaga
    17881 aggagattct tccagtactt tttgattgtt gcgttgtaaa gaacaatctc gctcgcctaa
    17941 atgaatgaca tgaccaaatc gatcacctaa gatctgaact tctatatggc gagcaggata
    18001 aatgattttt tccaaataca tatcatcatt tccaaatgcc gctgctgctt cttgctgagc
    18061 tgaagaaaaa tgctgtggga gctcttcttt tgattgaact ttccgaatgc ctttcccgcc
    18121 accacctgca gctgctttca gcatcactgg ataaccaatt ctgtctgcta tttccaatgc
    18181 ttcttctact gtagataata caccagtact tcctggaatg actgggacat ttgcttcttg
    18241 catcaattga cgcgcattga tcttatttcc cattgcatcg atcgttgcac ttttaggacc
    18301 aataaatgtg atattgcact cttcacacat ggaggcaaat cggctatttt cagataaaaa
    18361 accaaatcct ggatgaattg cttcggcttt tgtcacgatt gcagcgctca gtacttcctg
    18421 aacattcaga tatgaatcaa cggcttttgc cgggccaatg cagattgctt catctgctaa
    18481 ctgtgtatga agtgcttcgg catctgcttc tgaatatatc gctacggttt ggatccctag
    18541 ttcgcggcat gcacgaatga tacgtacagc gatttcaccg cggttagcta tcaatacttt
    18601 agaaaacata tttcacctat ccaatcataa aggttaattc cgcttcggct acttttttac
    18661 cgtctacttt agcgattcct ttgcctattc cggctgaagc tttcactttc aatatctcaa
    18721 cttcaaggta taaggtatct cccggtgtaa ctttcttacg gaattttgct ttgtccaatc
    18781 cgccgaaata agccgtcttt cccttgaatt gttcaagaga aagtaatgca actgctcctg
    18841 cttgagccat tgcttcaaca atcaataccc ctggcataac aggttcttct ggaaaatgtc
    18901 cttgaaaaaa gggctcattg atcgttacat ttttcttagc aacgatccgt tctccttcaa
    18961 ccatttcttc cactcgatca atcaaaagca ttggataacg atgtggaatg atttctttta
    19021 tttcttgtat gttcattgtt ttccctcttt cctgctttgc ttatttttgc tgaatcctaa
    19081 agagtggctg tccatattcg acaactgact cgttttctac aaggatttct gtgatcactc
    19141 cgtcggcctc actagttatc tcattcatca gtttcatcgc ttcaataatg cagataactt
    19201 ctcctttggc aacttgatca cctacttttt tgaaagctgg tttgtcgggc gctggttgca
    19261 gataaacgat tcccacgatt ggcgaaacaa cctcgattcc atcaaatact gccgattctt
    19321 tttctttcat tatttctgga gcagttgttg cttgttcagg tatgacattg actggctccg
    19381 aaactggcaa cgtttcgttt gtttgaacca tattcatgcc tcgacttgtt ttatttttat
    19441 tcatatacaa ttcaaattga ccttcacgca aatcgaattc cgttagcgag gattgatcaa
    19501 attgactgac taattctctg atttcattga tatccaaaat tattcctccc agcgtttcat
    19561 gcacagaacc gcattatgtc cgccaaaccc taatgaatta ctcattgcat aagtaaaact
    19621 tgcaggtttg ctttcgtttt tgacaactgt caagtcaatc tcaggatctt gtttttctac
    19681 attgatcgta ggtgggataa attgatgttc taatgccagc aaagttgcca aggcttcgat
    19741 cccgccggct gctcccaata aatgtccagt catcgatttc gtgctgctga cgtaagtatc
    19801 tctgaaattt tcacctaatg cgatttggat tgcttttgct tctgcctggt cattggctgg
    19861 tgtacttgtt ccgtgagcat tgatatatcc gacttgttcc ggctgtacac cagcttcttc
    19921 catcgcaagc ttcattgctt tagatgcacc agaaccatct ggagtaggag ctgtcatatg
    19981 gtaagcgtca cagtttgcac cgtatccaac gatttctcca tagattttgg caccgcgagc
    20041 ttgtgcatgt tctaaagatt caagaactag aacgcctgct ccttcaccca tcacaaaacc
    20101 attccgattt tcgtcaaacg gaatcgaacc acgattagga tcagttgatt ctgagagtgc
    20161 tgttaaagct gcaaatccag caatccccat ctcacaaatc gttgcttctg ttcctccagc
    20221 aagaatgaca tcagaatagc catgtttgat gttacggaat gcttccccaa ttgaattcgt
    20281 tccgctagca caagctgtta cgatagctgt acaaatacct tttgcaccta cacgcaaagc
    20341 aatatttcca gctgccatgt tggcaatcgc cattggtacg aacattggag aaactcgttt
    20401 tgcacctttt tcatgtagac gaataacttg gttttcgatt gttggaagac caccgatccc
    20461 agatccgatg atgacgccaa accggtcttg attcattttt tctttatcta aaccgctcat
    20521 ctccattgct tctaaagcag cgtagattcc atagatcgaa aacatatcca ttcgtttgct
    20581 atctttttta ataaaatatt tatcgtaagg gaaatctttg acttcacccg ctaaggtaat
    20641 ccctgtttct gaagcatcaa atttagtgat cggtgcgatg ccattcgtcc cctcttttaa
    20701 gctattcaaa aagctgtctg cgtcatttcc aataggtgaa gtgactccat agccagtaat
    20761 taccactcga ttcatttgtt tgctctcctt ttcttatccg tgcatgacca atccgccatc
    20821 gacattgatg acttgtccgg tgatatatgg attttgtgct aagaaaactg ctgtttgtgc
    20881 aatatcttct acttgtccga aacgctgcat cggaatttgc ttttccgctt gttccttgac
    20941 tttgtctgct aaaacttccg tcatatcagt tgtgatgaat cctggcgcaa tggcattaca
    21001 tgtaatgcct ctagttgcag cttctcgtgc cacagatttt gttaatccta ctacgccagc
    21061 tttactagct gcataatttg cttgaccaat atttccaatc aacccagata cactagagag
    21121 attaatgatg gccccttctc gctgcttcat catttttttc aatacgtgct gcgtcatatt
    21181 gaaggttcca atcaaattga tatccagaca ttttttgaaa tcttctgcat ccattcgcat
    21241 cacaagtttg tcatttgtga ttccggcatt gtttactaac acatggatcg atccaagctt
    21301 ttcttctgct tctttgatca tttgtcccgc tttctcataa tcagagatgt caccggaaac
    21361 tcctacgcat tttacaccga atgcttcgat ttcttctatc ttttcttttg gaatctctcc
    21421 gcgaccattc aaaatgatat ttgctccagc ttttgcaaaa gctaaggcca tcgctttgcc
    21481 gattcctctt gtactacctg tgataaatac attttttcct tttacatcca taacgttcct
    21541 ccttatagta ttgctattgt ttcatcaaat gtctgcttat tctcaacacg cagaactggt
    21601 attgttttat cgattttttt cataaatccg cttaatactt ttccggggcc gacttcgatg
    21661 acttgttcga tgccgattgt ttttaactta tggatactct catagaaacg aacaggttgc
    21721 atgacttgct gttcaagtag tcccgcgatt gtttctttct tcattatttc cgttgttgtg
    21781 ttgcttatga taggaaaaga aggttcagag aattgaatct gacttaggtc cttcgccagt
    21841 ttttttgctg ccggttctaa aatagctgta tgaaaaggcc cgctgacatt tagagggatc
    21901 attcttttga accctttttc ttttaatagt aaaacagctt cgtctactgc tttttcttcg
    21961 ccaccaatca cgatctgctg aggtgtatta taatttgcag gagatacgat gccgtacttg
    22021 ctagcttcat gacagctttc ctcgatcgtt tcaatcggcg cattcatcac tgcaaccatt
    22081 ttcccgcttc cagctggggc agcttctgtc atataagcgc ctctttttgc tactaacgca
    22141 actgcttcag tgaaggatag cgcattacta gctactaatg ccgaatattc tcctaaactc
    22201 agtcctaaag ctgcatctgg gatgattcca tgctccttca ataagcgata atacgcgatg
    22261 ctgactgtta gaatagctgg ctgtgtatat tgcgtctgat ccaaacgttc attttctgta
    22321 aagcaaagtt cagccatatc gtagccgaga atctcacttg cttcatcaaa tgtctgcttt
    22381 acaacggctt cttcatataa atcttttccc ataccttgat attgtgcacc ttggccacta
    22441 aataagaatg ctgttttcat gtttttctcc aatatatcca gaatatatgt ttgattcttc
    22501 ctattcaaaa aggccgagac attcctattt caggaaatgt cccagccccc aacctaattt
    22561 taatgattat gcagtttgtt tttctacgta agtcactaaa tcttgaaccg ttttgattcc
    22621 gtcttctgtt tcgattttca cgtcaaattc atcttcaatt tcattgatga tttggaataa
    22681 acccaagctg tcagcttcta agtcttcttg gatgtcagta gtcaatttga tttcttcagt
    22741 ttcttttcct aattcttctg cgatgatttc ttgaattttt tcgaatacca taataatata
    22801 ttcctccgat attgtttgct ttttattttt ataatttaac agtctacaac tccatgagaa
    22861 tacttcccca tgttaaacct ccgccatagc ctgtaaatat tactttctgc tgttttccta
    22921 aggtgatctt tccactagaa actgcttcat ccaataagat agggatgctt gcagcagatg
    22981 tattcccata tttatccata ttggtcagga acttttcttg cggcatcttt acttttcggg
    23041 caattttctc aataattctg aggtttgcct gatgcaaaag cagataatca actggtttgt
    23101 ctttcatgac ttcttggata ttgtttgcaa cgtctcgtac ggcaaaatca aaaatatctc
    23161 tgccagtcat ttgcaaataa tacgaacctt ctgaatcttc tgaatagaac ggatttttat
    23221 tttgctggta gccagcggtc aatgagctac ttctggtacc gtcagctgct aatttttctt
    23281 tcagcaattt tttctgtgga ttcgcttcta agagaatccc acctgctcca tcgccaaaca
    23341 acacagcagt cgtacgatca gaccaatcga ttagcttaga tagtgtttca ccaccaatca
    23401 ccagtccctt agttgagccg cagcggatca ttttttcagc catgcttaac gcataaacaa
    23461 atcctgagca cgcagcactg atatcaaaag cgatagcatg agttgctccc aatttccctt
    23521 gtacttgtgc tgctacagat ggtgtcgtgt aatcgggcga cattgttgct actaaaataa
    23581 aatccaaatc tttagggttt agatttttct gttttatcaa tttttccgcc actaaaatgc
    23641 ataaatcaga agtctcctga tttgttacac atcttcgttc acgaataccc gtccttgacg
    23701 aaatccattc atcagaggta tccatcatct tggaaagatc attattagta ataatctttt
    23761 caggaaggac gctggctgtt gcagtaatca ttccaaattg ttccattaaa tatcctcact
    23821 tatagtcttg caagaagtcg tgcaagtttt tcagtgcatc cagcaaggca tgctgttcct
    23881 ccgtagccat tccgtctaag atgttcttga ccatttcacg atgaaaatgc tgatggaccc
    23941 gaaatagtaa ctttcctttt ttcgttagtc ccaactttac aacgcggcgg tcatcttcac
    24001 tgcggatacg ttcgacatag ccttttttta ctaacttgtt aatagcagtg gtcaaggtcc
    24061 caacagtaat tgatagttct ttggctactt ctgaagttgt ttttttcttg tacatcccga
    24121 ttgcctcgat cgtgtgcatc tctgtgattg acagatcttt gaattgagat tttttcagct
    24181 cagactcttc gattgtcaaa atatcattga agacactaac aagataatca tttactgttt
    24241 ttaaatctgg ttccatgttc caccgtccac taattagttt gattatcaaa tcttttgatg
    24301 ttcaaactat atcttaactt ttttcaaaat gcaaccttaa aagatcattt ttttactata
    24361 tgagcttttt ttaagatttt actcatttaa aacaactgat aaatcagcat ttataatttc
    24421 ttttattaga aaaatcatct atctcacata ttttgattat caaagtattt tctgtgaaca
    24481 gaactataga attccctaaa acttgtccat tatttcaaaa tttgttatag tctttttatt
    24541 caaagcgtaa aaggagatcc tatgttaaaa gaaaaaatga aaactctccc catgctgccg
    24601 ggtgtctatc ttatgaaaga ccgagatggt aaggtcatct atgttggtaa agcaaaacgg
    24661 ctcaaaaata gagtaagcag ctattttcat cagaataaac agcattcaaa aaaagtatta
    24721 cgtatgattc atcatataac agattttgat ttcgtcgtag tagacacgga attagatgct
    24781 ttattgctcg agtgtcaatt gatccaacat taccgtcctt tttataaccg acaaatgaat
    24841 tatttcagta attataatta tgtacatatt accaataagg gatttgtatt aacagatact
    24901 cctacggctc gaacatacgg gccttttcgt ctctataaaa aaatgccgtc tattttgcgt
    24961 ataatggaag aaacttatca aatgccttgg ttgtctgaaa ttagtttgtt agctttgcga
    25021 gtccagcttc ctgacctaca ggaaatgact tttgaacaga aaaaaaagga actccaaggg
    25081 ctgtttcaag gccgaaataa aaagctactg acttacttga aaaaaagaca acaacatttc
    25141 atctatcagt tgaatttcga aaaagccggt atgcttcaaa aagatatcga attagttact
    25201 tattttatta gacggatcca ggaacaaaag cagtttttaa gaacaccatc gctcaccttc
    25261 agcatgccat tagcagcaga tgagagtcag aaaaagcact acctcgtttg ttatggtcaa
    25321 cttgcagaaa caatgattac ctctggtgac gtttctcctg acttctatta tgaaaagaaa
    25381 gaagctcatt tatctttgaa acgtcaattg tcaaaagaag aaatcgatcc tgtacaaatc
    25441 ttgatcagtt accgcaaaaa attagaaaaa gaacagtttg aaattgaact gttaaacaaa
    25501 aaagaagctg aaaagcaact aaattagttg cttctcggct tcttttaggc atcttcaact
    25561 tcaaatgtca attatcctaa aatttattgg attaagtcat agatttccat cgctacgata
    25621 tcgatattat caaattcgta ttgctgatga gtatctgttt cagttaattc aaatgtatcg
    25681 gtcgctttat cataagttac tgtacacttc tctacgcctt ctttttcaaa acggcgaact
    25741 tgcagttcat tatctgctga ctcgaccata gcctccagtc gttttatgat tgcgactaat
    25801 tgtgaatgtg tcatcggagg tcctcctttt cttatttctg ttatattgta tcaaaagcat
    25861 agcctacttg cattgatttt ctctaaaaaa actcgttttt tatgattttt tttctggtat
    25921 ttttccttca ggccaccaaa gtttgatcaa agcttgggtt ccgtcattct caaaaccttt
    25981 atctgccaac tcttcatata gtttcaatgc ttgggtagtt gctggcaata caagatccat
    26041 tttttctgct tcttctaatg caatctttaa atctttgata aaatgtttga caaaaaaacc
    26101 aggtgtgtaa tcttctttta aaatacgagg agaataattg cttaaagacc agttagctgc
    26161 acttccacct gaaagcgtct caatcacttt ctttaaatca agtccgtttt tttgtgcata
    26221 gacaagcatt tccgtcattc ctgtcatcgt acctgcaatc atgatctgat tagccatttt
    26281 tgtatgctga cctttcccag cagaaccatg caatgtaaat gtcgtaccaa attctttgaa
    26341 taaaggcagt acttgatcat aaacggcttc ttctccaccg accatgatcg tcagtgtacc
    26401 attctttgct cctaagtcac caccggatac cggtgcatct aacgcatctg cgcctttttc
    26461 ttttgctgtt tttgcaattt tttctgccag gcttggtgta cttgtagtca aatcaactaa
    26521 aatatgtcct gagatatctg cagaaaaaat ccccgactgt ccatagtaga tttcttctac
    26581 atctttgggg taacctacca ttgtaaagag aatattactt tgttctgcta ccgcttttgg
    26641 tgtatctgcc cagatagctc cttctgttac taaaggatca gcttttgctt ttgtccgatt
    26701 atagacagct acttcgtgtc ctgcttctag tagatggcgt gcgacagcac ttcccatgac
    26761 gcctgttccg ataaaaccaa gtttcatttt ttcgacctcc taaaaatcaa aaatccttgc
    26821 ttctatgcta gtatacgcta gaaacaagga ttttttcata aaaaatgttt gaattttatc
    26881 ttttaaatga atttaatttt attttgattc gttcaatcga gtcttcttct tcttttcttc
    26941 gcatctcttc ttcatgataa acaaccatgt cttgataaaa ttcgatcata gcttcgccga
    27001 aatgtgtaga tgaaatatca taaagtttag cttctaaaac ttttggatct ttaggaactt
    27061 cactggaaat ataatcaata agtgctggtg caaagtcttc gtcggtttca aaagttttcc
    27121 ctaatgaagg atgatcgaac aatcggttca aataagcatt tccttccacg acacaaggtg
    27181 ttccggctgc catcgcttct gtataagtaa gcccttgtgt ttctgaagta gaagcactga
    27241 caaaataatc tgccgcttta taataaagtg ctacatcatt gttgtccact tctcctgtga
    27301 actgcacaaa ttcttctaac tgcagttctg agatcaattc ctctaactct tctacataag
    27361 gtccttttcc tacgataact aatctggcgt taggataagc ggagatgacc tgaggaaacc
    27421 cattaatcaa ggcttggata tttttctcat aagaaatcct gcttaaagaa agaatcatca
    27481 aatggttttc ctgaagacct agtgactccc ttaatttgct gatatcctgc tgagtgatat
    27541 cagaacgttt gaatttttca atgtcgatac cagtaggaat cacgcgaagc ggtgctatta
    27601 caccatagct gcgtaatgta tcaataacac gttcacttgg acaaactaca cctgtcgtat
    27661 gatttgcaaa taatctagac aagtatttta catgggattg cctgatcact tttcctttac
    27721 cgatataatg aaggtagtct tcatacatcg tatggtaagt atggatcaga ggtattttca
    27781 acttcttagc taccatcttc ccaagtatcc ctgctccgaa ttctgtatgg gtatgaataa
    27841 gatctagttc tagttctttg gcaaccaaat aagcatacca catgcctcta acaacaactc
    27901 ttcgatcctt gaaagaaata aatggtacac ttggcattcg aatcacatct ttttccaact
    27961 tatccgcatt aggatcggtc gttgtaaaaa tatagacttc atgcccatgt ctttctaatt
    28021 cttgttttaa tgttttgatc gatgtagcaa ctccgctgac ttgcggaaag taggtatcgg
    28081 taaaaaagcc gattttcatg tttttcgcct ccaaactagt ccttgacctg atttactttt
    28141 tttaatttgc tagagacttt ttcattcagt acactttcgt atacattttt aagttcttca
    28201 ccgattttat tgatacttcg ctcttttgct gtttgataac cggcttgtgt cgtactcggt
    28261 agttcttgat tggtaatctg ttggatcagt cggataaatt cttcgtttgt cttccccata
    28321 tagcagttct tgctatcttc taaccagcca ttatacactg gaatatctcg aacaagtacg
    28381 ttttgtttgc ttgctaaagc ttctaaaaca acgatccctt ctgtctcttc ataagaaggg
    28441 aaaaagaata gatctgcacc tgaatacgca ccttctatga tctctccacg aatgtaacca
    28501 ggaaacagga cattttcagg atgatcttct tttacaattt tccggatgtt ccgaggaatc
    28561 gagtacatgg gaacatgacc aaaccaaata aatgtgtatt cgggcatctt cttagcaatt
    28621 tctacgaaat caatgattcc tttacgttcg aaaaacaaac cgacacaaat gattactttt
    28681 tgatccggag acagtttgaa atattctctg aacttttgtt ctttcattgg atcagggtga
    28741 tatttctcta agtcgatccc attggaaata gcttgaatcg gcacacggat accatagctt
    28801 tccaacaact gttttgaata aggtgttggc gtgatcagat agtccgcttt ttggtacaat
    28861 cctaccagat atttttttac gataggagaa agctggttcg atccgataaa ggaatttcgg
    28921 aaatcttcct ctgtagaatg cgcatgatag acaacttttt ttccttctct tctggcttta
    28981 ttgaccatat tatgactatt gattccgtat gtattgatgt gcaggatgtc ataatcttct
    29041 ttttcatcta gtgtataaga gataccgact tccgtcagag cacgtttttg atgatcaagc
    29101 gcacgcccga ttccagattt tgcaagcatc ttttcacttt caaaatataa taataccttc
    29161 accttacgtc ccccttcact tatatagtat aacatagcct acaaactgtc actcaaactt
    29221 aagccctatt ttt
//
LOCUS       DARBFD010000025        28637 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000025 DARBFD010000000
VERSION     DARBFD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.025"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            7..624
                     /locus_tag="SCK08_001089"
     CDS             7..624
                     /locus_tag="SCK08_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1200996.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="SCK08_001090"
     CDS             1644..2813
                     /locus_tag="SCK08_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1200997.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="SCK08_001091"
     CDS             3286..3840
                     /locus_tag="SCK08_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1200998.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="SCK08_001092"
     CDS             complement(4013..4486)
                     /locus_tag="SCK08_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1200999.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5034
                     /locus_tag="SCK08_001093"
     CDS             4498..5034
                     /locus_tag="SCK08_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1201000.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5074..5445
                     /locus_tag="SCK08_001094"
     CDS             5074..5445
                     /locus_tag="SCK08_001094"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1201001.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5729..7000
                     /locus_tag="SCK08_001095"
     CDS             5729..7000
                     /locus_tag="SCK08_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1201002.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7245..8270
                     /locus_tag="SCK08_001096"
     CDS             7245..8270
                     /locus_tag="SCK08_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1201003.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8564..9673
                     /locus_tag="SCK08_001097"
     CDS             8564..9673
                     /locus_tag="SCK08_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1201004.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            9756..10652
                     /locus_tag="SCK08_001098"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="SCK08_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10668..11492
                     /locus_tag="SCK08_001099"
     CDS             10668..11492
                     /locus_tag="SCK08_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1201005.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="SCK08_001100"
     CDS             11524..12834
                     /locus_tag="SCK08_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEG1201006.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="SCK08_001101"
     CDS             12982..14241
                     /locus_tag="SCK08_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1201007.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="SCK08_001102"
     CDS             14251..15123
                     /locus_tag="SCK08_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1201008.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="SCK08_001103"
     CDS             15135..15965
                     /locus_tag="SCK08_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1201009.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="SCK08_001104"
     CDS             16005..18188
                     /locus_tag="SCK08_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1201010.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="SCK08_001105"
     CDS             18249..19469
                     /locus_tag="SCK08_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEG1201011.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="SCK08_001106"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="SCK08_001106"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEG1201012.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="SCK08_001107"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="SCK08_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="SCK08_001108"
     CDS             22431..22640
                     /locus_tag="SCK08_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1201013.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="SCK08_001109"
     CDS             22633..22782
                     /locus_tag="SCK08_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEG1201014.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22936..23601
                     /locus_tag="SCK08_001110"
     CDS             22936..23601
                     /locus_tag="SCK08_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1201015.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="SCK08_001111"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="SCK08_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEG1201016.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="SCK08_001112"
     CDS             25304..25537
                     /locus_tag="SCK08_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEG1201017.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="SCK08_001113"
     CDS             25534..25941
                     /locus_tag="SCK08_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1201018.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27078..27596)
                     /locus_tag="SCK08_001114"
     CDS             complement(27078..27596)
                     /locus_tag="SCK08_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEG1201019.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         9843 a         4314 c         5245 g         9235 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagatata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc atatttttac
    26101 tataccctat acttttactt ggtatattta tgaataatcg caaatttacc cctatactac
    26161 tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc
    26221 tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag
    26281 taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc
    26341 tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcggtact gggattaaac
    26401 taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat
    26461 tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga
    26521 tctcttcagt atcagtaata ggataggcga taggccacat accctttgat ggttagaata
    26581 aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa
    26641 ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca
    26701 gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta
    26761 aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc
    26821 ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga
    26881 agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc
    26941 tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga
    27001 acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct
    27061 tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa
    27121 gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt
    27181 ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt
    27241 gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc
    27301 aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat
    27361 ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt
    27421 agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact
    27481 tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta
    27541 gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata
    27601 ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt
    27661 ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga
    27721 tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa
    27781 aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta
    27841 aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta
    27901 agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga
    27961 aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg
    28021 cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc
    28081 agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc
    28141 gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc
    28201 atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga
    28261 tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga
    28321 aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt
    28381 ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt
    28441 ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt
    28501 ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg
    28561 gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg
    28621 aactaccccc acttagc
//
LOCUS       DARBFD010000026        28056 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000026 DARBFD010000000
VERSION     DARBFD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.026"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(205..1104)
                     /locus_tag="SCK08_001115"
     CDS             complement(205..1104)
                     /locus_tag="SCK08_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEG1201020.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="SCK08_001116"
     CDS             complement(1114..1470)
                     /locus_tag="SCK08_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1201021.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="SCK08_001117"
     CDS             1825..2121
                     /locus_tag="SCK08_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEG1201022.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2644..3597)
                     /locus_tag="SCK08_001118"
     CDS             complement(2644..3597)
                     /locus_tag="SCK08_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1201023.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3620..4375)
                     /locus_tag="SCK08_001119"
     CDS             complement(3620..4375)
                     /locus_tag="SCK08_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1201024.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4372..5331)
                     /locus_tag="SCK08_001120"
     CDS             complement(4372..5331)
                     /locus_tag="SCK08_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1201025.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5324..6274)
                     /locus_tag="SCK08_001121"
     CDS             complement(5324..6274)
                     /locus_tag="SCK08_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1201026.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6424..6876
                     /locus_tag="SCK08_001122"
     CDS             6424..6876
                     /locus_tag="SCK08_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEG1201027.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(6965..9232)
                     /gene="gshAB"
                     /locus_tag="SCK08_001123"
     CDS             complement(6965..9232)
                     /gene="gshAB"
                     /locus_tag="SCK08_001123"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEG1201028.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9419..10417)
                     /gene="ftsY"
                     /locus_tag="SCK08_001124"
     CDS             complement(9419..10417)
                     /gene="ftsY"
                     /locus_tag="SCK08_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEG1201029.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10428..11237)
                     /locus_tag="SCK08_001125"
     CDS             complement(10428..11237)
                     /locus_tag="SCK08_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1201030.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11246..14827)
                     /gene="smc"
                     /locus_tag="SCK08_001126"
     CDS             complement(11246..14827)
                     /gene="smc"
                     /locus_tag="SCK08_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEG1201031.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14847..15533)
                     /locus_tag="SCK08_001127"
     CDS             complement(14847..15533)
                     /locus_tag="SCK08_001127"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEG1201032.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15881..17662)
                     /locus_tag="SCK08_001128"
     CDS             complement(15881..17662)
                     /locus_tag="SCK08_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1201033.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17688..18602)
                     /locus_tag="SCK08_001129"
     CDS             complement(17688..18602)
                     /locus_tag="SCK08_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201034.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18629..19591)
                     /locus_tag="SCK08_001130"
     CDS             complement(18629..19591)
                     /locus_tag="SCK08_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201035.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19591..20535)
                     /locus_tag="SCK08_001131"
     CDS             complement(19591..20535)
                     /locus_tag="SCK08_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201036.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20536..21540)
                     /locus_tag="SCK08_001132"
     CDS             complement(20536..21540)
                     /locus_tag="SCK08_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201037.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21808..22050)
                     /gene="acpP"
                     /locus_tag="SCK08_001133"
     CDS             complement(21808..22050)
                     /gene="acpP"
                     /locus_tag="SCK08_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1201038.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22172..23173)
                     /gene="plsX"
                     /locus_tag="SCK08_001134"
     CDS             complement(22172..23173)
                     /gene="plsX"
                     /locus_tag="SCK08_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEG1201039.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23222..25258)
                     /gene="recG"
                     /locus_tag="SCK08_001135"
     CDS             complement(23222..25258)
                     /gene="recG"
                     /locus_tag="SCK08_001135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEG1201040.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25375..27057)
                     /locus_tag="SCK08_001136"
     CDS             complement(25375..27057)
                     /locus_tag="SCK08_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEG1201041.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27109..27471)
                     /locus_tag="SCK08_001137"
     CDS             complement(27109..27471)
                     /locus_tag="SCK08_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1201042.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27741..27929
                     /locus_tag="SCK08_001138"
     CDS             27741..27929
                     /locus_tag="SCK08_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEG1201043.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7756 a         6069 c         4930 g         9301 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtggac ttattgccga
     2281 gaagcagcag atggaacatc gtttaaagaa gagatcggta caaaataaaa atatctaaat
     2341 ttaagtttta tctaagtttc gctttgtacc tttgattcaa atcaaaggag gcgatcttga
     2401 tgctgaaaaa atggatcaca ctattttttg ccactcccca ttctagaatc aacccgtatg
     2461 atgtagataa tgaattcacg ttgaaaaaag atttctcaaa taaataaaaa ccatccgtaa
     2521 aatcatcaat acgattttac ggatggtttt tgataacttc tttgaggata ggctcttgct
     2581 atttaagaat gtaagaaaca ttattgttac atagtatgag agtttaatcc tctgttattt
     2641 atgctattta aatgcttgat tcacatcttc gatcatcagt ttcattgatt ctaaaccgcc
     2701 cccgcttaaa taccagacat ctggttcaag ggagatgatc tgctgatttt taccagcatt
     2761 cgtttgtgct accagttcat tggcagaaat atcatctttg ctgtcgtctc caccaatagc
     2821 ttttgttctg tctactacaa ataagatatc cggatttttt tccagcacat attcataaga
     2881 aacactttga ccgtgagtgg atgcttcgat ttgatcatct gcttgttcaa agccaaataa
     2941 atcatggatg aaaccgaatc tagaaccaga tccgtaggct gataattgcc cttcattcac
     3001 aagtgtgact agtgcttttt tgtcagtagc ttctgctttc tttttcgtct gatcgattgc
     3061 actagataat tcctctaatt gagttttagc ttcttcttgc ttaccaaaga tctcccctaa
     3121 agcagtgaca ttttgctgga aagattccca tggatttttc gcatctaaag aaaggaaaat
     3181 ggtcggagca atttgactta gatctttttg aaaatctctt tgtcggccag aaatgatgat
     3241 tagatctggc tgaagctgat taattttttc taagtctggt tcttttattc cacctgctga
     3301 ttctactttt ttataggatg aaagatactc aggaaggctg cttgtagctg ctccaactac
     3361 cttatctcca acaccaagag catccatcgt atctaaagaa ccattgtcga atacgacgac
     3421 tttttctgga ttatttggca cctcgatttg gtctccatta ctatctgtta tcgtcaatgt
     3481 agtttcagtt tgtgtgctgc ttttttttgt gtctgcctga ctggtgttgt tgctatttcc
     3541 acatgctgct aaaccaaatg atgctagaat caagatacct actactaatt ttttcatctc
     3601 ttctctccta atgttagttt tagaaataaa gacagaatct gcggccttca atctcacaaa
     3661 tccgtatgtc catttcgtat aattcatcca atacatcttt ttttatcatt tcttgggtcg
     3721 ttccttgcgt aaataactgt ccatctttca ttgcaacaat ttcgtctgca taacttgcag
     3781 caaagttgat atcatgaaga acaataacaa ttgttttccc gatttcttgt actaaatttt
     3841 ttaaaatctg catgatttga accgcaaaat tcatatctag attgttcaaa ggttcatcca
     3901 gtaaaatata atccgtgtct tgcgctagca ccattgcaat atacgcacgt tggagttgac
     3961 cgcctgaaag agtgtcaatc gattgatccg ctatttcttc taacccaagg tgttccagtg
     4021 attcctgaac gatttcctcg tcttttgtcg tcaattttcc tttcgtataa ggaaatcgtc
     4081 caaattctac taattctttc acggtgattt ggacatttat ttcattcgat tgtcgtagga
     4141 tcgataattt tttggctaat tcattggatt tccaagcctt tacttcgtta tggtctaaat
     4201 agatttcacc tgtatctttt ggtatcaaac gactgatcat cgacaacacc gttgactttc
     4261 cagcaccatt tggaccgata aaagcagtca atttcttttc aggaatcgtt aactgtacat
     4321 ctttaaccac tgattttcca tggtacagtt tattgacctt ttgtaaatca atcataggat
     4381 actcttcctt tcgcgaatca attttgtgac aaaataaatt cctccgacaa attcaacgac
     4441 gacactcact gttgtattcc agccgaaaat ctgctctact gcgagctgtc ctcctgtcag
     4501 aaacagcata gctaccagaa agcttccgat aaatagctga ccatgacgat aagtagaaaa
     4561 gacacgataa ctgatatttg cgacaataaa ccctaaaaaa gtaactggac ccacaagagc
     4621 cgttgatgtc cctgtcaaaa gactgacaca acataaaatc aaaaattcaa aacgatctgt
     4681 gtctatcccg aggtttactg cttgttcatt tccgagatgg aaaacgtcta gcacatgtcg
     4741 atatcgaaat aacaggtacc agcagccgat aatgatcaaa gaagcaggca aaagatactg
     4801 actctggata ttgccaaagc ttgcaaataa tctgccttga agcaaatcat attcattcgg
     4861 atccatgatg acttgtaaaa aggtactgac actaccaaaa agcgtaccta aaatcatccc
     4921 tgacatcagt aataaaaaaa gattcgctct catttttttc aaaagaaaga aaaataacaa
     4981 tagactcagc ccgaccatca gcccaacgtt caataagaaa aaaactgtac ctgtctgctg
     5041 aagcatcgtc actcctccga taaagaagaa aaggaccgtt tggacaagga catataacga
     5101 ctcaagtccc aatactgaag gagtcaaaaa ctggttatgc gtaattgtct gaaagctgat
     5161 tgtggcactg ctgacagcta gcgagacaag gataaatgca aaaattttct tactcctcaa
     5221 tgcacatgca aaatgccagt ttccatatgt atggatcgtc aagtacattc ctgtaacaaa
     5281 taaaacaaga acaagcatac caataaaaat agatggcttt tttttaagca tatcgtcgtc
     5341 cccttatcag aagataaagg aagatagccc caccgatgat tccaacgatc acactgactg
     5401 gtatttcata aggaaaaatc aacacgcgag cgacaagatc gcaaaatact aagaacaaac
     5461 tacctgctaa agcagttacg ccaattgttt tgtgcacttg atctccatat attctagaga
     5521 caagattcgg aatgatcact ccgataaaag gaatgcttcc taccatgatc aacatgactg
     5581 cactagccaa tgatactaag ccaatgccta aaaactgcat cttctggtaa tctattccca
     5641 gatttactgc aaacgattcg cccagaccaa tgatcgtaaa tcggtaagca aatagccaaa
     5701 ccaagagcaa caatggaaca acagcataca ataactcata atttcctcgt atcacagtcg
     5761 caaaattccc ttgaagccat gaagtcatat tttgtaccaa ctggaattga taagcaaaaa
     5821 aagtagcaac tgaaccaatg atattcccta acatgactcc aatcaacgga agcatcaatt
     5881 gatttttctg gaggaacaat cgcgtaaggg ataaaaaaag caatgttcct gcaaaagcaa
     5941 agcaaaatgc tgtcaaggat cggacaatca aggatgcacc aggtaggaaa atcatagcca
     6001 ctaaaatacc taaacgcgca ctatccatcg tgcccgcagt cgttggtgaa acaaatttat
     6061 tttgggtcaa atgttgcatg atcaacccac aaatactact ggtcgctccc acaaggatca
     6121 aacaaatcgt tcgtggcaaa cgagtcacta gcaagatttg taagctttgc ccatctaact
     6181 ggagcagttc tgacaattgt acattcgata ctccgatgaa caatgaaata ctacttaaaa
     6241 taactaataa tgctactagt cgtccgtagc gcatccaatc cacctttcta attgagaatg
     6301 attttcattc ttattttaag tgaatcgttg aacaaaagca accgcttttt catatgtttt
     6361 aggtattatt gggaatatgc tataatcgta agaaagacaa agagaagaag gagggtattt
     6421 tttatggcac aagatgatgt aaaaagccat ttggataagg gactttatgg tacaccttta
     6481 gtgaaccctg aagaacagca taaatattta gggacattca gggaacgctg ctatcttagt
     6541 atgactgttg ctgaaatgat ccaaccaaaa aacaaagaaa actttttaaa agaactcaaa
     6601 aaacatccag atgcttccat actcttgaat ggggctctac ccctttctgt ccaaaaccaa
     6661 tatatccagc tagcaacgaa aaacaatagc cgtttcactg tagtcaacga ttacgtcact
     6721 gcaaatcctg aggaattcgg attgctctta acagcaaaag aagcagtaaa tgaacctgtg
     6781 attgatatcg aaaaaaaata cccagaagaa aactcttcgc cagctgaaaa agaaaaacca
     6841 aaaaagaaaa aaagcttctg ggatcgttta ttttaaaacg aaaaaacaca taaattttta
     6901 catgcaaaaa aagcttttga tcagattgac atcaaatagg acaatctgtc aaaagctttt
     6961 tcttttaaaa tacttcagga tataacaatt tcaatacatt catcgtcaat cttctgcctt
     7021 ttcctgcaaa aggataaaca tgcatatgca tggctggatt gaaatttgct tcgatgatcc
     7081 catatgcttt tttatcagtt gtaggatcga tttctttatc tggtataatc aaatcgatac
     7141 cactaatctt agctcctagt gcttctaccg cagaaacggc gatttttttg taagcttcag
     7201 aaaattcatc agtcatatcg atcgaatcac cacctgtact gatatttgag ttctccctta
     7261 gataaacaat ttgattggct tggggaactg attcgatcgt caacccttgt tctttcaaca
     7321 tcaattgttc taattcacct aattggatca attctaaagg tgcacgatga ttcgttccac
     7381 gcaaagggtc actatttttt tcttcaacaa gttctttcac tgttcggata ctgtcaccaa
     7441 taacattagc agggactcgc aacatgatgg cttggacttc tccgtcgatc acaaagaaac
     7501 gatattccgt tcccggcata aattcttcta ctaaaaccga actgtcttca cgaaaagcaa
     7561 tggctaaacc tgcttgataa tcttccagac tagctccttc cttgaaaatc gtgataccta
     7621 acccgtaatt ggttgattta ggtttgatca caaatgcctg ctcggcaaat ctcggataag
     7681 cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc tggtacccgg aacccagctt
     7741 cttttaagac ttttttcgtt actgttttgt tttccatgat aagtgggacg atatagctgt
     7801 ccttacttgt catgtttgca tttttcacat attctacatg gtcttggaat tgcagacgca
     7861 aaaattgatc cgactcatct aatactttga cttggacacc tttttggatg gcatcgaaca
     7921 taaatatctg agtagaaagt tccatttcgc gaaaaccggc caactgatag ggacgttcat
     7981 ggctttcttt ataatattga tgtcccaatg ccacagcaac ttcttgctga ctcgatagct
     8041 gtgcttcttg atagagtttc gctgctaacg tttgttcagg atgatccatt tgagtcaatg
     8101 cttcctctac taaataaaga gaatccaacc aatccaattc aaccagcatt tgcttcattc
     8161 cttcaagaat ttctctacct tcttgatgaa attctgtttg atctgatggt tgttctagtg
     8221 ctactttgtt atttcgagtc gtaccttctg ctacccattg gtcacagtct tctttttctt
     8281 ctgtccataa catgtacatc aggaataatt gtaagaaccg aacttgctct ggattgatac
     8341 ccaatcttgc ataaggattc aaatcgatat tccgcaattc aatataacga acacctgttg
     8401 ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag cggtgtataa aattctttct
     8461 cctccgaaag tttcccttct tcgatcattc gctcgatatc tgctaaatac tgtttcatag
     8521 aagcatagga aactttcaca tttggatgat tcgtatagcc cagcgcactg ttgcgaatac
     8581 tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta gttcttctca ctcattggtg
     8641 aagcaccgaa taaataagtg atcatccagc gatagcgcat aaagtttctc gctgttttca
     8701 aataaagttc tgttttgaat tcggaaaaat cttgaaattc ttcctgatgg ctgaacaatg
     8761 ttcttagcaa ctcatcgcca aattcaaaat tgaaatgaat accgcttacc atctgttttc
     8821 gcttcccgta ttcttttgct aaataacgtc gatacaagac atcttcgaaa ttttttaatt
     8881 ttgcaataat gatctcttcg tctttttccg gtaaggcagg tggcatactt aatggccaga
     8941 tcatctcttc tttcggtata gaacgagcag tcacatcata aacagcagcg agatactgaa
     9001 ataattcggg aatagaatcg gtaacagggg tgatcatctc tgtttgtgtt tcgctaaaat
     9061 ctgtttggat ataggggtga taggatcgat cgccaaagat tgctggatga tcggtttttg
     9121 ctagatttcc tgcaagatca acccgctgtc cttcccgttc tataccaaat cgagcttggt
     9181 cgataaaagg gcgcgcattg acatgcaata ataattgctt aaaattcatc attaagtgac
     9241 ctccaattca tatgaatcta attataatca aaaagtctaa gaaaaagaac gaaattgttt
     9301 gagaaccgct tacaaataag tataaaaatg aaggcatcaa aaacacagct atgttaaaaa
     9361 caatcattca aacaaggagc tgcaactttt gtcacagctc ctgcttttta gacatgggtt
     9421 attcttcttt tagtaatcct ttgaacaatc caacgacaaa atcatttgga tcaaacggct
     9481 ccaagtcatc gattccttca ccaagaccga ccaatttcac tggtaaatgc aattcattac
     9541 ggatagcaag tacgataccg ccctttgctg taccatctaa ttttgtcaaa acaagaccgg
     9601 tcacatctgt tgtttctttg aattgttttg cttgaaccat cgcgttttga ccagtggttg
     9661 catctaatac aagaagtgtt tcttggggtg cttctggtaa ttcccgctgg attactcgtt
     9721 ttattttttc taattcgttc atcaagttca ctttattttg cagtcggcct gcagtatcaa
     9781 ctagtaaaac atctgcctgc tctgtctttg ctctttccaa agcatcgaat acgacagctg
     9841 ctggatcgcc tcctgcattg ccacgaacaa cttcaacacc agcgcgttcg ccccagacaa
     9901 ctaactggtc gattgctcca gcgcggaagg tatctgctgc tgccatcaat acttttttac
     9961 cttcgttttt gaactgatgg gcaagtttac caatacttgt tgttttccct acaccattaa
    10021 caccgacaaa caaaataact gttagtccgt cttcttggat atttagttca tttacttcat
    10081 tgattccttc cgcttcatag agatccacaa gtttctcgat gatcgcattt tggacttctg
    10141 ccgatttttt cgcatttctg agtttcactt cttgacgtaa tgcttcggta attcgtatcg
    10201 ctgtttcaaa acctacatct gcaccgataa gtgtttcttc taattcttca aaaaagtctt
    10261 catcgactgt tcgaaaattg gcaaacaact cattcatccg ttcaccaaat gttttacgcg
    10321 tcttttctaa acccttgtcg tacttctcct gtacttcaac ttcttctgtt ttctcttcgc
    10381 cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg actcccccta ctctaataca
    10441 aatttttgga tcactttggc tacgccattt tcttcattgc tggctgtcac gaaatctgct
    10501 gcttctttga cgaaaggcac agcattctcc atcgctacgc ctagtccagc atattggatc
    10561 atcggtaaat cattttcctc atcaccgatc gccatgattt cttcttgttt caaaccaaga
    10621 tcttttgcca ataaagaaat accataagct tttgtaacac cttttggcat aaattccaaa
    10681 agcatacttc ttgttttgat gatctcatac atttcatgga attctgctgg tatttctttg
    10741 attttaggat ctaattcttc ttggtcataa gcgattactg ccttattaag cacaaattca
    10801 tctgtaatat cgcttatatc cgctggttgg aattgcagca gcttattcaa cacattgtac
    10861 aaggattgct tccttggggc actaggcagc tgtaaaacca cattgtcaga caaaacatct
    10921 agtggcagat ccaattgctg agccagtttg tataagcgtt ggatatctga tacttccatc
    10981 actttctttt ccatgatttc accagtatca tttttttgta ccaaaccgcc gttgaaagtg
    11041 atgctgtagt caccttcatc taccagtcct aattcttgca gataatgcgc catcgcagct
    11101 aaggggcgtc ctgtacatag aacgatcttg accccttgct gttttgcttg tgctaatgct
    11161 tgtttattct catctgaaat cagcttttgt tcatttaata atgtaccgtc caaatcgatt
    11221 gctactaatt tgatcatttt cgttcctaca ctttcgtttc gttgattttc gcctcttcat
    11281 tgatatcttc catccgtaca gaaacgattt ttgatacacc ggattcctgc atcgtcactc
    11341 cgtacagtac atctgctgcg accatcgttc ctttgcggtg tgtgactacg ataaattgtg
    11401 tgtcatcttg gaaatcgctc aagtaacgac caaaacgttt cacgttggct tcatctaacg
    11461 ctgcttctac ctcatctaag atacaaaacg gaacaggaca gacacggata atggaaaaga
    11521 gcaacgcgat ggctgtaagt gctcgttctc ctccagaaag caaacttaga ctttgcagtt
    11581 ttttgccagg aggttgtact tcaatctcaa tacctgtttt cagcaaatca gttggttccg
    11641 tcagtactaa ttcagcttta ccgccgccaa acatatttgg aaagacaatt ttgaattctt
    11701 gacggatagc ttcaaatact tctttgaatc ttgtccgcac ttcatcatcc atctcatcca
    11761 tcgtttcaaa caattggttt ttcgctgcta acagatcatc tcgctgtgtt gctaagaatg
    11821 tatgtctttc actgacttgt tcgtattgtt cgattgaatt taagtttact ggtcccagtt
    11881 tttcaatctg ttcttttaag gatgaaacct ttgttctact gtcttcgata tcggttgttt
    11941 cttgatagtc ttgggaagct ttctcaaatg tcagctgata ttctgactgc agataggtca
    12001 aatgattatc taaaatcatt tcagctcggt ctttttggac ttctaatttg gattgttccg
    12061 acagtcgtgc tttttgctca cgattacgct ctgccaagac tgcttcaagc ttatcgatct
    12121 ccttttgcct tctatctcgc tgctctttgg cttttgccaa ctcggctttt aatgtttctc
    12181 gctgagcaga taattcgtta atttgttttt ccaaactttc ttctgtgact tcatgatcag
    12241 aaaagtctgc tgtcaatgtc gccaactgct tttctatcgc ttcttgacgt tcggttgcct
    12301 ctgctttttg gactcgggca ccgcggagct ggatctgcag atgattaaac tgctctttca
    12361 agacagcaag atctgcttgt tcttgtgctt tttgactttg tacttgtgct cttctggctt
    12421 ccatctggtc gctttcttga cttaacgatt tgatttcttc gtcgattttt tgtcgttgcg
    12481 attcaagatc tgtttgtttg tctttcaatt cgttcgtttg cttttcgtaa tcttcgataa
    12541 actgctgcag ttcacgtgtc tcaaaagttg agatttgctt ttctttttca aaacgttcaa
    12601 gttcatttat gatgttttgt agctgatttg tcgcttcttg ttcttcaaaa cgtaattgtt
    12661 cgcctcgtgt acgcaaaact tcttgttctt cggctaaacg agctgtttct tgttgcagct
    12721 cctgaacttt tttttcttgt gcttggagtt gtttatctgc ttcttcaaat tcactagtca
    12781 actgtttcaa ttcttgattt tgtacaaaca aactgccttg gtttcctcgt ttattcgctc
    12841 cacctgtcat ggatcctccg gcattcatca catcaccttc taaggaaacc actcgatatt
    12901 gatagcggat cgtttgcgcg attgcattgg cactagttaa gtctttggct aaaaggatcg
    12961 ttcctagcag gttttgaacc acagtttgga tttgatcggg ataactgact tgttcactgg
    13021 caatccctat aaacccttct acagctgctg cttgtgtcaa tatatgagcc ggtaattgtc
    13081 ttggtttgat tgtcgtcagt ggaaggaatg ttgctcttcc tcctctttgc tgtttcaaat
    13141 acgtgatggc ttgccgagca tctttttcat tttcaacgat gacatgctgg gctgccccac
    13201 ctaaagctgt ttcgatagct aacgtgaaat ctgcaggaac atcgatcagt tcagccactg
    13261 ctccgacaat acccgaaagc tgctgtttat gctgcaagac caatcgaacc ccttggtaaa
    13321 aaccaaaata gttttcttgg atttcttgaa ggcttttctt acgagcacgt agctgctgga
    13381 cttgattcat taattgatac attttaggct gttcttttgc taatgcttct tggaactttt
    13441 tcccgttttg ttggatcatt tctaacttct cttttgtttc agttaaagat gattttagct
    13501 gcgcctgctt ttgtgtcaat tcttcttttt gtaagatcaa tcggtctact gatgcttcta
    13561 cttccgattg cttggctaac gtctgcttgc tttttgctgt ttcttgtata tattgacgtt
    13621 ctagatactt cagttcgttt cctacagctg cttgttcttg catcaaatca acatattgat
    13681 ctcttaattc tgccaataat tctttcgttg atttttggta tttttctaat tcttgctgtg
    13741 tttttatgag atttgcttca gctttttgga tctctgtttc tttttctgct gctgctttca
    13801 tcaagctttc ttgaagcttt tcaaaatgtt taaccttttt ttgtgcttct gctaatgaag
    13861 tttgatattc ttgcgagctt ttttgtgtat gctttgtccg ctcctgcaaa acatcttttt
    13921 gtccttctgt ctgcttcagt ttttccgaaa gatctaataa tacttgctga tttttttcga
    13981 tcagacgatc ggcttgcgca ttttctttac gctgcttcgc caaaatggat tcttgttctt
    14041 ggatcgactc agatagtttg cctaattcta gattgaattt cgcaagctgt gcttgtttgt
    14101 tgtcccaatc tttttttgct gtcttgattt cagcaaccat caaagagaca tcggtttgag
    14161 tcagtgtttc ttttagccgt aaaaactcct ttgctgcctc actctgggcg gccaaaggag
    14221 ttaactgttc ttccaattcg tggatgatgt cttggacgcg gctgaggttg tcttctgttt
    14281 caaaaagctt ctgttctgct ttctttttcc gctgcttgta tttcaagaca ccagctgctt
    14341 cttcaaagat cccacgacgg tcttccggtt tactactgaa aatcgcttct acttttcctt
    14401 gagaaatgat tgagaacgat tcttttccta atccagaatc taagaacaat tcttggatat
    14461 cttttaatct gcagctttgt ttattgatga aaaactcgct ttccccagtc cgtcgataac
    14521 ggcgggttac actgatctca ttgtactcca atggcagata atgatcacta ttgtctaaaa
    14581 tcactgtgac ttctgcaata ttcagctgct tacgggtatc ggaaccggca aagatgatat
    14641 ccggcatttt tccgcctcgc agacttttag cagattgttc tcctaatacc cagcggatag
    14701 cctctgtaat attgcttttt ccgctgccat ttggtccaac cactgctgtg acgctatttt
    14761 caaaatcaat gactgtcttg tctgcaaatg atttaaaacc ggctatttca attcgtttca
    14821 aatacaaagc tttccctcac ttacttttac agtgctgcca atgcattttc cgccgcagct
    14881 tgttctgcta attttttcga tttcccttgt ccagtgccaa tcaattgatc atctacataa
    14941 acttcgatcc aaaatactcg ttcatgagct ggaccttctt cattgatcaa tcggtattcg
    15001 attgatacat cacctgattt ttgcaagact tcttgtagtt ttgttttatg atccatctca
    15061 tgtgaaaaag cacctgcttt gactttagga aaaatcactt tttcgatgaa tgcatgggtc
    15121 gtgtcgaatc cctggtcaag atataacgcg cctaaaaaag cttcgaatag gtcgcaaagc
    15181 aatgctggtc gcgtacgtcc tcctgaattc tcttctccct tgcccaacaa aacatattga
    15241 tcaaagtgac attctttggc aaatttcgac aagctgtctt cgcgaacgat cgcagctcgt
    15301 gtttttgtca attttccttc tggcaaatga ggatataaac ggaataaata ctgagaaacc
    15361 atcagttcta aaacggcatc tcctaaaaat tcaagtcgct cattatccga taattgcaga
    15421 ttgcgatgct cattcacata ggatgaatgg gtaaaagctt gttcaagcaa atttagatcg
    15481 tgaaaaacga tgttatagtt ttcttttagt tcttttgtca gctggttgtc cattttccac
    15541 attcctttta tggatatttt ttcgatactt ccttattata ctggtttttt gtcaaatttg
    15601 aaatggttcg gtcaaaaggt aagtaaagct ttaataatcc tagtgattga cgagaagttg
    15661 tgaaaaattc gtttagtttc gagtatgaag aaaaaatttt gtgaaaatgg ctttccatat
    15721 ttttgcataa atttgactta ataccgagaa actggacttt gaacaccgtt tattggagaa
    15781 gtcatgaaat cagcgccttg acctgaatat tagagaaata aacagaaaaa tatcaaaaaa
    15841 ccgaaaaagg acatcgccct ttttcggtca tttttagtga ttatgaacgt gaatctgctg
    15901 taacagaaac atttgcccat gggtttggta catcataaga ccaatcccag tctttgacac
    15961 ggtcgttgac tggcaatact tcattacgga acaacgttgg aatcgcaaat gcttcttcaa
    16021 atgcatattg ttgccatttt tgatatgctt ctttttggta ctcttcatca aatgctttgc
    16081 ttgaactgat atcgttgatc aattttgtat tttcttctga ttcataacga gtgtagttga
    16141 acgcagcatt tggtccccat agtccacttg gatttggatc agttcctgta ttccaagcag
    16201 cttggtaaac atcaatttca ggatcatcgt tttctaattt atcatagaaa gaattgaatt
    16261 cgatcagacg acctgttgtg tattggacat ccaaaccaat ttctttccat tgttggatat
    16321 agtaatctgc caatggttgc gctgtttccc caccggacat cgaagcaaag ttgattgtta
    16381 gtttatttcc atctttgtct tctcttaaac catcgtcatc cacatctttg tagccagcat
    16441 catctaatag ttttttcgct ttgtctagat cttgtgtgaa tcctttcatc tcatcatcat
    16501 ggtatgactg gaatactgga ggaatcaatg aagtcgcatt gcttctcaaa cctgcataga
    16561 aacgttcacc tactgcatta ttatctaatg catagcccat tgcttgacgt aatgatttat
    16621 cagccatttt tgaatctgga ttgtattcca cactctttgt atctgcattc catttaccta
    16681 acttgaatcc tagataagta taagaagttt gttcacggcc taagtttgta tagccagcaa
    16741 tttctttata ggtatcgtaa gtatctgttg gcatttggaa aatcatatca tataatttag
    16801 agttcaatgc ttcagttgca cttgccatcg gtacagattt catcgtgatc tttttcaatt
    16861 taggcgtacc attccagtag tgctcgtttg gcaagtattc tactgattct cctgcaacga
    16921 tattactcat gtaataaggg ccaaaagtca ctggattttt acgtacttga tcgcttgatt
    16981 ccatatcttt gattgctaca ccttctaacg tatgttttgg tgctgcatat gaccagatac
    17041 caccacccga ttgaagcatg cttgggttta cttctttata ttcgatttca actgttttgt
    17101 cgtctatttt tttgattccg gagattgtgt cggcacttcc atcatgatat tcttccatac
    17161 ctacgatgtt acggaaagtg tcgtcataac ggataccagt gtaatcttta ttcccaatga
    17221 tttcgtaagg gaaaatcaca tcgtccgccg ttacatcttc accatctgac catttgacgt
    17281 tgtcttttac tttgattgtc gcttttttcg tatcttcatc tagatcaagc gttgcagctc
    17341 cgtcgtctgt gattctgaaa tcgtcatcgt ttgcaaatag cggttcatga gaaggagcca
    17401 taaaagctgc atcatatgaa tcttggtaaa actcccactg gaacaatcct ttaaactgac
    17461 tgtccgttgc gaccgcagct tctaattcgc cgtcttcgat tgcatcatca ttattttttg
    17521 ttgcaattgg aaaagttaat tcctctttgt tattgccgtt gcctccagag ttgtttccag
    17581 aggtatctgt acctccgccc ccgcatgctg caagaacgac agcagacatc agcgctgcaa
    17641 atccaaaaat cgttttcttc attcctattt cccccttcgc ttctatacta gcctaaccgt
    17701 tggcgcgcat ctgcagaacg cttgaatgct tgtccaacat aattgatact caacatcaaa
    17761 actaaaataa ggattgatgc gggtaaccag atccattgcc gattcactaa gacttctccg
    17821 ttacttgcat aaccgatcaa tgtacctaaa ctaggtacat ttgccggcaa gccgaaacct
    17881 aagaaagtca aagttgtctc gataccgatg tttgccgcaa agttcatcgt caaattagta
    17941 atgattaatg aacttagatt cggcatgatt tcgcggaaca tgattttaaa atcattggtt
    18001 cccattgttt ttgaggcact gacataatct cttcttgctt cagataatgt cttgcttcgg
    18061 aataatcggg ctttagctac ccaataaaat gcactcatga tgaatataaa ggaccagaca
    18121 ttgtatctgg aaataatcga tacgaagacg ataatgatca gcatgatcgg tagaatcatg
    18181 ataaaatcaa cgatccgcat caagatgttg tcaatgatcc ctccgtagta gccagagatg
    18241 atccctaatc caaccccgat gatcgaagta aggatcgtga tcgcaaatcc gatcgtaaca
    18301 gagtttctag cacctatgat caattgtcct aatacatcac gtccgccttc atctgctccc
    18361 agaatgaact tttgtccttg tgtcgaaact gttcctggtg cagcgtattt gtcgaagata
    18421 ctaacataca tcactttgtc ttgatcggtc agcatcgcac cgataaagat cactaataat
    18481 aaaaccacta gcaaacctaa tgaaaaaata gcaattttat cttttttaaa ttctcgagca
    18541 atcattcgta tccccatagg aggaatactt tcttgaactg ctgtattgtt tttttcactc
    18601 atcgatccat tctcctttct gattggtttt attgaatccg aatacgcggg tcaacgatac
    18661 tcatgatgat atctgaaagt aatgtcccca ctagcgtcgc gattcctaaa atcaatacta
    18721 aggatgtgat cacggtatag tcccgttggc cgatggaatc aataaatagt tttccgactc
    18781 ccggataacc aaaaatcttt tcgataacta cagaaccagc aatcaacgaa gtgatttcat
    18841 accccatttg agaagccatc ggcaacgctg catttcggaa aatatgtcga ctgaatactt
    18901 tattcgtcgg tacccctttg gaacgggctg ttctgacaaa atcgagcgat ttcgaatcga
    18961 ttacttcact tcgcagatat tggattgtga cggcagtcgc caaaaatgct tgggtaattg
    19021 atggtaacag aatatatttc aaacggtcta tccattgagt gataaatcca ccttgcaaac
    19081 ctagtgtttg tgatccactt gttgggaacc agcctaaacg ataaccaaaa acaaatagca
    19141 tgatcaagcc gaagataaat aaaggaacag caaaactaaa gaagttgtag attacgacaa
    19201 tcttatccaa gattgaattt tgataccggc cagcaagcat ccccagaggc acagcaatca
    19261 aataagtaag gacaactgtc aagatcgata gatataaagt gttgatgatc cgttcgccga
    19321 tcaagtcagc aacagagcgt ttgtataaga aactttgacc aaaatcgcct tgcaaagcat
    19381 ttccgatcca acggaagtac tggacatacc atgggtcatt caagccagct gcttgtcgca
    19441 tctgttcgat cactgccgga tcctgatttg gattgatcaa accggtaaat ggatcccccg
    19501 gcatcgcttg ggctaataag aagatcagaa cacttaaaat aataacttgc gggatcatca
    19561 ataaaacacg tcgcagaatc gttttccaca ttagcttgca cctccatctt tcaacgccac
    19621 tttgtgtgta gttgtgatcg tacgcaaatc gtagactcga ccagtcgcat catagtattc
    19681 tttttggttt tggatatact cttgttctac tcttcgtcga ttttctttgt ggctttccct
    19741 attttctaca tcgatttgcg gaatagctga aagcagacgt ttcgtataga tatgtcgcgg
    19801 gtcattataa atgtcttctc ttgtaccgat ttctacaaat cgacctttgt acataattgc
    19861 gatattgtcg cacatgtgtt ttactacacc taaatcatgt gaaatgaata aatagctgag
    19921 gccgtattct tgctggattc gtttcatgaa attcaatact tgcgcttgaa cagatagatc
    19981 cagagcagat acaggttcat cggctacgat cagttttgga ttggtagcaa cagcacgggc
    20041 tactcctaga cgttgacgtt gtccgcctga aaattcatga ggatatttat atagcgcatc
    20101 ttccggcatc ccaacgatat ctagcagtcc ttttaccttt ttcttttctt cttgatcgct
    20161 taaccgttca aaatttcgga tgggttcagc aatgatatcc aacactcgct ttttaggatt
    20221 caaactggac attgaatctt ggaagatcat ctgcacatcc ttgttataac ccattttttt
    20281 gcggttgctt cgcttcgtta catcttttcc ttcatacatg atttgtccgg ctgttacctt
    20341 ttctaatccc acaacggctt ttcccgtagt agattttcca gagccagact ctcccactaa
    20401 accatatgtc tttccttttt cgataataaa atccacacca tctacagcat atacatgatc
    20461 cgttactcga ttgaagaatc cactgcgaat agggtaatga actttcaggt ctttgattgt
    20521 gattaattct gtcatctaca cctctccttt ttcgtctcta aagtggaaat gcttatagca
    20581 ggagcaacgt accaagtggt tcggtgcgac ttcatgaagg ataggctctt cttcatgatc
    20641 gcttgctggt atccatggaa tacgtggtgc aaaccgacaa cctttacgag gcatgttttt
    20701 cagagaagga acgacacctt cgatcacatg gagttctgca tcatgatttc cttcttgtgg
    20761 tatagagttc agtaaagagc gtgtataagg atgcttagga tgatcaaaca gttcttttac
    20821 acctgctact tcaacgaact gtccgccata catcactgcc actcgatcag ccatttctgc
    20881 tacgactcct aagtcatgag tgatcaagat gatccctgtt tttgtttctt cctgaaggtc
    20941 tttcagtaaa tcaagaatct gtgcttggat ggttacatct agggctgtcg tcggttcatc
    21001 tgcaatgacg attggaggtt tgcaggcgat tgcgattgcg atgatgacac gttgtctcat
    21061 accgcctgat agttcatggg gatattggcg tccaactctc gcaggattag gaatccctac
    21121 ttgttctagc aattctagtg cacgagcttg acgctgttct gcattcatct ttgtgtgata
    21181 ggaaagtcct tctttgatct ggtcttcgat tctcatgaga ggatttaatg cagacaacgg
    21241 atcttggaaa atcattccga tatcatttcc gcggatttta ttatagagcg tttcgttcag
    21301 gtcgattaaa ttcagatcgt tatataaaat ctcacctgta atttttgtgt tattaggatc
    21361 aagtaatccc ataatcgtag tagccaaggt acttttcccg cacccagatt ccccaacgat
    21421 tgctaaaatc tcgtttttgt ctaaagtaaa tgaaacgtca tctactgcat cgtaatagtc
    21481 atctttgagt cggaatcctg tatgtaggtt ctgaacttct aataattggt tatcggacaa
    21541 tcgaataccc ctctctgctt cctaaatgga agtttttctt aataaaaaag gacattacac
    21601 agaataaatc gttttataac aaaggttatt cttatctttg tgtaatgaat tattaataat
    21661 tatatagaaa accgctttac tttgcaacat aatttctgtt tatcgccaga attatcagaa
    21721 aaatgacttt tacgtgtcaa aaagattaaa aaaagaagga taaaaagctg catgcagctt
    21781 tttatccttc tttgctttta tttgaaatta tttaagatgg ctgctgatat aatctactgc
    21841 gccaccgact gtttcaattt gttcagcgtc ttcatctgaa atttccgtac cgaattcatc
    21901 ttcaagctct aaaacgaatt ccataatact aattgaatca gcttttaaat catctttgat
    21961 gttcaattca tccgtcactt tatcggtatc tatctcaaaa tgatttgaaa taattttcgc
    22021 tactttgtta aatacttctt cgcgagtcaa ctttttcacc tccaagaatc atacacttgt
    22081 caattaccta caccatggct aattttactg gcatttttca ggaatgtcca cctttttttc
    22141 aatggaacgg atcgcttcag atatccaaat actattcttc gcttttttca aattgttcga
    22201 ctaattgtcc aactacatct gtctcaagca ttgtgtggat ttggcggatc gtatagcgaa
    22261 cagcatcggg gcctgttgct ccatgagttt taatgactgg tgctttcaag ccaaacaaaa
    22321 cggcaccgcc atgtttggaa taatccattt ctgctttcaa agaacgtaag ccgtctttta
    22381 aaagcatagc gcccattttt cctttgatcc cttcatttag aatagcagat tttaatagat
    22441 tcatcatatt cattgctgtt ccttcaatag atttcaaaac agcgttgcct gtaaatccat
    22501 ctgtgaccac tacatcagcc acaccattga gtagctcacg agcttctacg tttccaataa
    22561 aattcaagct tgtttcattc tgaaggagtt cataggcttt tttcgtcact tcactacctt
    22621 ttgttgcttc tgttccgttg tttaagagtg cgacacgagg acgtccgata cctcggactt
    22681 tacgtgcata aaaagaacct aaaataccgt attgcaacaa atgttctggt ttgttttctg
    22741 cgttagcgcc aagatccagc atatcgaaac cggcaccttc tttccctact actggcaagg
    22801 tagacatcaa gccagggcgt tcgatatttt tgatacgtcc tacgatgaac aaccctgctg
    22861 ctagtagcgc gcctgtattc cctgctgaaa atacagcatc tgcttcaccg tttttcactg
    22921 cttgtgcagc aaggaccatt gatgcagtct tttttcgtcg aattgctttt actggctcat
    22981 catcactatt gattttttcg tctgtatgga taatcgtgat gtttttttca tttgttaagt
    23041 atttcttgat ttctgcttct tttccgtaaa gtaagaattc tacttctgga aaatcttgct
    23101 gcgctaaagc cacgccttct acaattgctt gaggtgcgtg atctccgccc attgcatcta
    23161 cggcaatttt cattgtttcc actccttttt ccgtcatcag tataaagtat agcacagtct
    23221 gctaatcgaa ataacgatta tcctgatttt ctatttggag gctttgcgct aaaggctgat
    23281 actcaggaag catctgccaa tcctttattt gccaaagggc tgcagcctct tctctggcta
    23341 cctccaaaac atttccatcc gtcaccaagt cagcaatcgc aaattgaggc agaccagatt
    23401 gacggaagcc aaatacttca ccaggaccac gtaattccag atctttttcg cttagtacaa
    23461 aaccattggt ggtctctgtc atgattttca tccgttccac acctagttca tttttaggat
    23521 tagcaactaa gatacagtag gaagcatcac ttccgcgtcc aacacgtcca cgaagttggt
    23581 gcagctgtgc caatccaaat cgatccgcat ccatgatcag catgacggtc gcgttcggca
    23641 cgtttacccc tacttcaatt acagtagtcg aaactaatat ctgcatctgg ttgtctttga
    23701 aggcttccat gattacttct ttttcctgat tcttcatttt accatgtaac aagccaactt
    23761 catattttgg tgcaaatagt tcacgaagtt tttcatagat ttccactgca ttttttacat
    23821 ctaatgcttc tgattcttca atcagcggac agatgacata catttgatgt cctcttgcca
    23881 gttctttttt cgtccaatcc agtacactgt ccaattgagg tgttcttacc cagcgtgttt
    23941 cgatcgggat acgtccagct ggaagttcat caatgataga tacatccatc tcaccatatg
    24001 ctgtaatagc taatgttcta ggaatcggag tcgctgtcat aaataagaca tctggtcgcc
    24061 agcctttttc acgaagtact ttccgctggt tcacgccaaa acggtgctgc tcatccgtaa
    24121 taactagacc taaattctga aattcgactc catcttggat caatgcatgt gtgccgatca
    24181 cgacgttaat ttccccttgc tctagttttt caaggagctc acgtcgttct tttgctttcg
    24241 ttgaccccgt aatcaatgcg atggttactt cattgggatc aaacagctgt tgcaagcttt
    24301 ccatatgttg ttgtgccaag atttctgtag gcaccatcaa cgcaccttgg aaacctgctg
    24361 taacggtagc gtataacgca atcgcggcaa ccactgtttt tccgctacct acatctccct
    24421 gcaaaagtcg ctgcatatgc tgcggactgc gaagatcccg acagatttca ttcgttacac
    24481 gtttttgtgc atttgtcagt tcaaaaggta gcttttgagt aaattgtttc aatttttgga
    24541 catcataaag gatctccaaa ccattcgttt ctgctttttc tgcttttttc aatccttgaa
    24601 gacgcatttg aaataaaaag aactcttcaa aaacgacacg tcgtttggct tgatggtttt
    24661 cttcgtggtc ttttggaaaa tgcatacttc ttacagcaga agctcggtca agcagtcgat
    24721 atttctccac aaggttgttt ggcagatttt cttcaatcaa tgtgccgtat tcttgaaacc
    24781 cttttttgat tagatcaact aatgtcgttt gacgaattga tttgttgacg tgatatatcg
    24841 gcgaaaagtc atccattgtt tgagaaccta aaatcttcat cccgttcaag gctttacgct
    24901 ttgcatccca tttaccgtat acggcgattt cctccgataa aatgactttt tcttttagat
    24961 aaggttgatt aaaaaaagaa acgttgaaca catcatgttc ttgcatcata cgaaattgca
    25021 atcggctttt tttataacca aatcgattca tcacaggagg cgagactacg attcctttca
    25081 aagtaacctt ttcttgatcc tggatctcgt ttagatttct ttcctgaata tcttcgtagc
    25141 gaaaaggata ataagtcagt aagtcttcaa tcgtttggat gcccaagctg gcaagactag
    25201 ctgctctttt ttctccgact cctgccagca tagtgattgg atcatctaaa gaacgcataa
    25261 atttttcctc cctcctttaa caaaaaaagg acttggtcag cgatcatacc tgatcactaa
    25321 ccgaaggcct tttatgaatg aatactgtgt tagaaagata ttctgacaca gaaattattc
    25381 agcagaaaat agataaggat agacaggctg atctccttgg tggatctcga tttccaaatc
    25441 aggatgtttt tctgttagtt tctcagcaaa ttcatttgct tgttcaactg ttccttcatc
    25501 acctgccaag atcgttacga tctctgtgtc ctcattgatc atttgttcta gtgtacttaa
    25561 cgctgttgct gtgatatctg ggttagagat caggattttt ccatcgatca tgcccaaaaa
    25621 gtctccttct ttgatctcta caccgtcaat tgaggtatct ctgatcgcgt gagtgacttg
    25681 accgctcaca acgctagaca acatttctgt catcgtttct ttgttttctt ctaaagaagc
    25741 ttgatcattg aatgaaagca tagctgtcaa tccttgggaa atcgttcttg aaggaacaac
    25801 tgctactggg atatctgcta cttctgctgc ttgatcagct gccatgaaga tgttcttatt
    25861 gtttggcaag acaatcactt tatctgcgtt tacttcttga atcgctttaa caatgtcttc
    25921 cgtacttgga ttcattgttt gtccaccact gataacataa ttagcgccta aactttcaaa
    25981 cagctcttgg acgcctttac cagcagcgat cgctacgata ccatacggga tacgtggtat
    26041 ttcttttttc gcaaactcgt tcacttcttc atcgtgttcc aaaatcgtct catgctgcaa
    26101 gcgcatgttg tcgactttga ttttgactaa tgaaccgaat ttttgtccat aattcaagac
    26161 ttctcctggg ttttctgtat gcacgtggac tttgatgatc tcgtcatcat tgaccacaag
    26221 taaagaatct cctataccat ctaaatagtt acggaattct tcgtaatcga atgtactatc
    26281 gactgtaggt ccttctccga tttttaccat gatttctgta cagtaaccaa aatggatatc
    26341 ttctgtcgcc atttgacctt ggacactgcg atgatgttct gcattgacca tatcatccat
    26401 ttctgctgga gaaggttcat atgtttcatc cgcttggaat tctccattca atgcacttaa
    26461 aaagccctca taaacaaaca gcagtccttg ccctccactg tctacgacac cgacttcttt
    26521 taggactgga agcaagtctg gagttttagc taaagcacgc tttgctcctt ttacaacagc
    26581 tgtcattact tcaacgacat catcagtact ttttgctttc ttttctccag ctttcgctgc
    26641 ttctctagct actgtcaaaa tcgttccttc tactggtttc attactgctt tatacgcagt
    26701 ttcaactcca tgagtgaagg ctgcagcaag atcagaagca tttaatgaga caacatctgg
    26761 aatttgctta gaaaatccac gaaacaattg agacaaaata acgccggaat ttccgcgcgc
    26821 acccatcagc aatccttttg aaagcattgc tgctaattct cctacttttt ctgaggttga
    26881 atctactact gccttagctc cactagtcat agatagattc atatttgttc ccgtatcgcc
    26941 atctggcaca gggaatacat ttaatgaatt gacgtattct gcatttactt gcagacgatt
    27001 cgcacctgcc tggaccattt cctggaactg gcttgcactt atttctgtta cactcaccta
    27061 gataatcctc cttcagatcg actgctgagt taccagccag tctcctgcct agtctggcag
    27121 tacacgaaca ccttgtacaa agacattaac tgaattggca gttacgccta ataatgcctc
    27181 tagattgtat ttcacttttt cttggacgtt gcgggaaact tcagagattt tcgtaccata
    27241 gctgataatc gtataaacat ctacagcaat accattatct tcttggcgga caactactcc
    27301 tcttgagtaa ttttctttac gcaaaatctc agtaatatta tctttaattt gatttttgct
    27361 ggccattcct acaacaccga acacatctgt cgcggcgcca ccaacaactg ttgcaattac
    27421 atcattggta atttcaatgg ttccagctga cgtttgaatt tttacagcca tgaatgagcc
    27481 tccttaacgg gtaaaaaact cccatttttc ttctatcata ttttatcata gcgcaacttg
    27541 aaataaaagg cagttaccca aagtactcaa aaaccttgca aaatttcagg tataaccttg
    27601 tttaatttac aattattctt ctcaattgtc aattgtatct atgaaaatgt acttgcaaat
    27661 tcccattcta tatgatatgt tagtctggta tgagctgaat tattctattg ctccaaatca
    27721 aggcaaagga ggaaattacc atggcaaaag tttgttactt tacaggtcgc aagacaaaaa
    27781 gtggaaacaa ccgttcacac gctatgaact caacaaaacg tactgttaaa cctaacttac
    27841 aaaaagttcg cgttatggtc gacggaaaac ctaaaaaagt ttgggtgtca actcgtgctt
    27901 tgaaatcagg aaaagttgaa cgcgtttaat tgaatgatac ttggaccgtt cagatagtga
    27961 acggtttttt atttaataaa aatttttaag ggtgggactt ttgattccta cccttttttt
    28021 gcgtttattt ttgttatacg aacaaattat gaagtt
//
LOCUS       DARBFD010000027        27700 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000027 DARBFD010000000
VERSION     DARBFD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.027"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            270..2036
                     /locus_tag="SCK08_001139"
     CDS             270..2036
                     /locus_tag="SCK08_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEG1201044.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2138..3397
                     /locus_tag="SCK08_001140"
     CDS             2138..3397
                     /locus_tag="SCK08_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1201045.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3544..4845
                     /locus_tag="SCK08_001141"
     CDS             3544..4845
                     /locus_tag="SCK08_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1201046.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4848..5699
                     /locus_tag="SCK08_001142"
     CDS             4848..5699
                     /locus_tag="SCK08_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1201047.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5712..6527
                     /locus_tag="SCK08_001143"
     CDS             5712..6527
                     /locus_tag="SCK08_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEG1201048.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6678..7844
                     /locus_tag="SCK08_001144"
     CDS             6678..7844
                     /locus_tag="SCK08_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1201049.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8074..8721
                     /locus_tag="SCK08_001145"
     CDS             8074..8721
                     /locus_tag="SCK08_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1201050.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8805..9749
                     /locus_tag="SCK08_001146"
     CDS             8805..9749
                     /locus_tag="SCK08_001146"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEG1201051.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9884..11335
                     /locus_tag="SCK08_001147"
     CDS             9884..11335
                     /locus_tag="SCK08_001147"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEG1201052.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11356..12192
                     /locus_tag="SCK08_001148"
     CDS             11356..12192
                     /locus_tag="SCK08_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1201053.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12192..12851
                     /locus_tag="SCK08_001149"
     CDS             12192..12851
                     /locus_tag="SCK08_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1201054.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12977..13666
                     /locus_tag="SCK08_001150"
     CDS             12977..13666
                     /locus_tag="SCK08_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201055.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13672..14628
                     /locus_tag="SCK08_001151"
     CDS             13672..14628
                     /locus_tag="SCK08_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEG1201056.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14672..15184
                     /locus_tag="SCK08_001152"
     CDS             14672..15184
                     /locus_tag="SCK08_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1201057.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15440..15988)
                     /locus_tag="SCK08_001153"
     CDS             complement(15440..15988)
                     /locus_tag="SCK08_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1201058.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16077..17258)
                     /locus_tag="SCK08_001154"
     CDS             complement(16077..17258)
                     /locus_tag="SCK08_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEG1201059.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17494..19002)
                     /locus_tag="SCK08_001155"
     CDS             complement(17494..19002)
                     /locus_tag="SCK08_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEG1201060.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19161..19352
                     /locus_tag="SCK08_001156"
     CDS             19161..19352
                     /locus_tag="SCK08_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201061.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19555..20472
                     /locus_tag="SCK08_001157"
     CDS             19555..20472
                     /locus_tag="SCK08_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEG1201062.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20465..21538
                     /locus_tag="SCK08_001158"
     CDS             20465..21538
                     /locus_tag="SCK08_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201063.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21686..22204
                     /locus_tag="SCK08_001159"
     CDS             21686..22204
                     /locus_tag="SCK08_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201064.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22185..22577
                     /locus_tag="SCK08_001160"
     CDS             22185..22577
                     /locus_tag="SCK08_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEG1201065.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22653..23588
                     /locus_tag="SCK08_001161"
     CDS             22653..23588
                     /locus_tag="SCK08_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEG1201066.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23721..24500
                     /locus_tag="SCK08_001162"
     CDS             23721..24500
                     /locus_tag="SCK08_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1201067.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24493..24810
                     /locus_tag="SCK08_001163"
     CDS             24493..24810
                     /locus_tag="SCK08_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1201068.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25054..26664
                     /locus_tag="SCK08_001164"
     CDS             25054..26664
                     /locus_tag="SCK08_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1201069.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26821..27669
                     /locus_tag="SCK08_001165"
     CDS             26821..27669
                     /locus_tag="SCK08_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1201070.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8978 a         4899 c         5552 g         8271 t
ORIGIN      
        1 ctagaatata cttctacgcc aaaaactgtt aaaaaacttt tactttagaa aattggagaa
       61 attgatcgct taagataaat tcaggacact aaatagtaca ggaagtagac taaaactttt
      121 aaaagtttta agtctatcta tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac
      181 tgttaccgat aacatttttg aaagcgttgt cttttgcttc tctatcactt atagtgatga
      241 cgtcatatta aaacacaagg aggaaatcaa tggatacagc tgcaatttat catcgaccgg
      301 aaagtgaata tgcatttcta tatacgaata caagatttcg tattcggctt cgtactagga
      361 aaaatgatat caaaaaagta tatgtgctat gtggagatcc atataccata acaactgaaa
      421 aatggtatca aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt catgattatt
      481 gggaaattga agtgacgagc gaaacaagtc gcttacagta tgcgttccat gttgttgggg
      541 aagataacac ggactgtttt tacggtgatc aaggaatttt tccttatcga gaaaatgtga
      601 ttactgaacc aaatttttat tttcgaatac cttattttca tcaaatcgat cgattcacta
      661 ttccagaatg ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga tttgcaaacg
      721 gggacaaagc aaatgacccc gaacacactt taccttgggg aagtaaagat ccagaccgag
      781 aagacttttt cgggggagat ttgcaaggtg tgatggatca tttggattac ttgacggacc
      841 ttggcatcaa tggcatctat ttttgtccca tatttaaagc cacatccaat cataaatacg
      901 atacgattga ttattatgag gtggatcccg cttttggcga taaaaaactt ttaaaaaact
      961 tgattgatga agcacataaa agagggatac gtatcatgtt cgatgctgta ttcaatcata
     1021 tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata
     1081 aagactggtt ccacatccgt tttttcccag tagattctta tcagatgact gacttaccag
     1141 aaactgctga aaatatccca tatgatactt ttgcttttac tccattcatg ccgaaactaa
     1201 atacagcaaa tccagaagtg caaaaatatt tgctggatat cgcaacttat tggataaaag
     1261 aattcgatat tgatggatgg cgtcttgatg ttgctaatga agtcgatcat catttttgga
     1321 aaaaattcag agaagcagtt acagaaatca aaccagacat ctatatcctt ggagagattt
     1381 ggcattcctc tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg aattatgcat
     1441 ttacagattc gattaaagat tattttgcaa aaaagaaaat cacagcttca caaatggtca
     1501 gtggaatgaa tcatcagcaa atgctttaca gagatcaggt gaacgaagga acattcaact
     1561 tattagattc tcatgatact gccaggatct tgaccctttg ccaaggtaac aaagaattga
     1621 tgaagtctgt gatggccttt atgtttcttc aaaaagggtc tccttgtatc tattacggga
     1681 cagaaatagg gatgactggt gaagatgacc cggattgccg caaatgtatg atatgggaga
     1741 aggaggaaca agatctagag ctattcggtt ttataaaaga acttgtttct ctaaggaaac
     1801 agttgtctaa gattatttct gaaggttcta ctcagtggct aatagtaaat gatcgtgaag
     1861 ataagctcta tttcacacgg aaactagagg gacagatcat ctatgtttac tttaatcagt
     1921 caaaagaacc ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt
     1981 tgttagaaga cgggaaagca gagatacgac aatacggatt tttgatcatg tgttgaaaac
     2041 tgttaccggt aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat tataataagg
     2101 gtgtaacagc acgttgaata caaaggagga aatgaagatg aaaaaaagaa gtgtgaaaca
     2161 gttgggttta gggttaatct ctttaggaat cttaggcgga ttagcggctt gtggcaatgg
     2221 taactcttca gcagaagggg aagccgatga aggaaaaacg ttaacagttt ccgttgattc
     2281 tggatataaa gattatataa acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa
     2341 aatcaagcta gtagaaaagg acatgttcga tcagttggaa tcattagcgt tagatgggcc
     2401 tgctggaaag ggacctgacg ttatgatggc agcatatgac cggatcggtg cgttaggaca
     2461 gcaaggacat ctggcggaag taaaactggg aaatgaagca gcttacgatg aaacagacaa
     2521 agcccaagtg acttatgatg gaaaaatcta cggagaacct gcagtaatcg agacccttgt
     2581 tttgtactat aacaaagatt tagtcgatac agcaccatca acgtttaaag atttggagaa
     2641 tctatccaaa gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata caggattttt
     2701 agctaaatgg actgactttt attattccta tggtttaatt gcaggttacg gtggctatgt
     2761 atttggtgat gatggcacaa atccttcaga tatcggtttg aataatgcag gtgctgtaga
     2821 aggaatctct tatgcaacag attggttcaa aaatgtttgg ccaaagggaa tgcaagacat
     2881 aaaaagtgca ggagacttcg ccagccagca attcatgtcc aataaaactg cagcaatcat
     2941 tgacggtcca tggcaagcgc aaacatacaa agaaaacaat atcaattatg gtgtagccaa
     3001 aattcctact ctaaataatg gacagcctta tcaaccgttt ggcggaggaa aaggatgggt
     3061 agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa
     3121 tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa accaacaagc
     3181 tagggaaaca gcaaaggcta aaaacgatga attaacaaca gctgtgattg aacaatatga
     3241 aacagctcaa gcgatgccaa acatcccaga aatgggcgaa gtttggacag gtgcagaaaa
     3301 cttgatgttc gatgcagcat caggttcaaa aacaccaaaa gaatccgcag atgaagcagt
     3361 aaaaacaatc agtgaagcca tagaacaaaa atactgatta aaatgataac cgtccatttt
     3421 tataaaaaat ggaacaggtg cttcagataa aagtggcaag agcgagatgg aaaattatct
     3481 tgctcttgtt gccattatct taaaagcagt ttgcggttta gcaagtttag agggggcatt
     3541 cagatgcagc aagataaaac aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt
     3601 attccaggga tggggcagtt tgtaaatggc caagctttta aaggtatcgt ttttttcgcc
     3661 atattcgtcc tattcattgg tcagatgctt ctgggcggat ttcatgcatt ggtaggattg
     3721 gttactttgg gaagtatacc aatggaagat cattcattat ttattttgat tgaaggcact
     3781 ttgcaattca tcattacaat cattttcttg attttttacg gtttgaatct tttagacgct
     3841 tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca atcggacgat gaaggaagtc
     3901 atacataata taggtgatga aggttttcct tatctattga cacttcccgc ttatcttctc
     3961 atgattttta cgatcatttt cccagtccta gttacattgt tcactgcatt tactaattat
     4021 gacttcaatc atattccacc agccagttta attgattggg taggggctaa aaactttttt
     4081 agtatcttct ttttaagctc atatcgtaat actttttttg cagtcttttc gtggaccgtg
     4141 atttggactt tatgtgcaac aactttgcaa atcacccttg gtattctcac agcggttgtt
     4201 gctcaccaga aatttattaa attcaaacgt attttcggta tcattttctt attaccatgg
     4261 gcggtcccag cttttattac tatcatgagt ttttcgaata tattcaacga tagcgtaggt
     4321 gccatcaata ctcaagtgat tcctttcata aaccagcttc cttttgtaga tatcagtgca
     4381 atctcttgga aaacggaccc gaactggacg aaagtagcaa ttatcttagt gcagggctgg
     4441 ttaggatttc cctatatcta tgtgatggtt acaggaatct tacaatcgat ttcagaagac
     4501 ttatatgaag cagcaaaaat cgacggagca aatgcatggc agcgcttttc aaacatcacg
     4561 cttcctgctg tcttcctagt ggcagcgcca acatttatta cacagtatac ggggaatttc
     4621 aataatttct ccatgatcta tctgtttaac aatggcggtc ccggaagcgt aggcggagga
     4681 gctggttcta ctgatattct tatttcctgg atctataaac ttacgactgg aacttctccg
     4741 cagtattcta tggcttcagc aatcacgttg attatctccg tcatcgttat ctgtgtgtca
     4801 ttgctggtat ttaaaaagac aaatgcgttc aatatggggg attaacaatg aaaaaatttc
     4861 attcgagtgc tcgattccaa aaacgacttt ctcacttttt tacttacttt tttcttttaa
     4921 tactgtcagt cattatcatc tatccgcttt tgatcacagc aagtactgcc ttcaagtcag
     4981 gaaatattgc agcttttagt ttagatcttt caagtgactg gactttagga aactttactc
     5041 ggctattcag tgaaacactt tacgggactt ggtataaaaa tacattgatc atttctgtcc
     5101 taacaatgat cgtacaagtg actgtagtaa ctttggcggg ctatacttat agtcgctatc
     5161 gttttatcgg ccgcaaatca agtttgatgt tctttttagt gattcagatg gtaccgacta
     5221 tggcagcatt gactgccttt tatgtcatgg ctttcttatt agaagcgctc gataagtact
     5281 ggttcttgac aatgatctat ataggcggtg gaattccaat gaatacttgg ctgatgaaag
     5341 gatactttga cactgtacca atagatttag atgaatctgc taaacttgat ggggcagggc
     5401 atttcaggat ttttttccag attgtattac cgcttgtcaa accgatgctc gccgttcaag
     5461 cattatgggc ttttatggga ccttttgggg atttcatgtt agctaagttc ctgcttcgaa
     5521 cacctgaaaa tcagactgtt gctgtcggct tacaaacatt tatttctgat gtgacaaacc
     5581 aaagagtctc tctttttgct gctggggcaa ttttgattgc actaccgatt tgtctattat
     5641 tcttcttttt acaaaagaac tttgttaccg gcctgacatc tggcggaaca aaaggatagg
     5701 agcagagtat catgcaaaca tttccgttaa actatttttc cgcattacgt acgccaactc
     5761 aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt tttcttgata tttgtatttt
     5821 tagtttcctt gatggtcatg cctgtgactc ttttttatgc caatcagatc caagcgattc
     5881 cattagaaca atttctatcc gttcattcgc tgatcgatga acaaggaacc caaaaatttt
     5941 ctgaacttga attatctgaa actgggttac aagcatcgca acaaacgata gctgtgaccc
     6001 ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca atccgatcat ggaactttta
     6061 ttgattttga gaaggaacaa tgggtgatcc aacaaaagga taaatctggt atacgccgct
     6121 atacgatgaa ttatagccca tctttccaac cagattctgt acgtacgccc gaagatttcc
     6181 aacgattttt agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt ttatcttata
     6241 gtttaagtct tggtcttgtc ctatttgtca tgacgagtct cattttattc ggggcttcct
     6301 ttttcttgtg gatgacaaga aaaagccaac tttcatccat tcatacattc aaagagagtg
     6361 caaatttgat gctgaatgtt atgggaatag gttccatggc agcagcagtt gtagggttta
     6421 tccactttga ctttatcttg atgctaagtg tccaaacaac gataactgtc ttgctgcttt
     6481 tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa gtttaagcga
     6541 tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa aaatttctga
     6601 aaaaattaaa cttcttagtt gattcttttc ttttttagta ttaacgtaat agggtagttt
     6661 ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa tcaattattt tcacgttctt
     6721 tctcgctccc tgtcgaagta aattattggg acggtacaac gaaacaatac ggagatacgg
     6781 acactcctcc ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa gagattacaa
     6841 gtaacgcatc tttggcacta ggagaagcct atatggatca tcgaatcgag atcgagggaa
     6901 gtatccaagc attgatcaac gatgcttatc agcaagcaaa cagcttttta cgaggaaaag
     6961 attatctaaa atggctgcca aagaaagaaa aacatacgaa acaaaaaaac aaagaagata
     7021 tccatgcgca ttatgactta ggtaacgact tttataaact ttggctagat ccaactttga
     7081 cttattcttg tgcttatttc aatacacctg aagatacgtt ggaacaagca caaatcaata
     7141 aagtccatca tatcttagac aaactcttta tcaaagaagg cgaaacgttg cttgatatcg
     7201 gttgtggctg gggaacattg atgttcactg ctaccaaaga gtataatgtc aaggcaactg
     7261 ggatcacact aagtgaagaa caatacgatt tgattaccaa aagaataaaa gaagaacatt
     7321 tagaagataa atgccgtgta ttattaatgg attaccgcga actaaaaggc gaaacgtttg
     7381 atcatatcac aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt gaaggttatt
     7441 tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc
     7501 gacaacaagg cggtgcgaca aatgcttgga tcaataaata tattttccct ggcggttatg
     7561 tccctggtgt tactgaatta gttgataata ttacaaaaaa cgatttgcaa ctgattgatt
     7621 tggaaagctt acgtcgtgac taccaattga cattagaaca ttggacaaag aattttcatc
     7681 aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg tgggatctat
     7741 atttacaagc ttgtgcagct tcatttgaat caagtaatat cgatgtgatt caatatttgc
     7801 ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc ataattgagg caaaagtact
     7861 gtttaaagac tatttaaaaa gaagctaagt tttatgatgc tccagaagtg aacttttttg
     7921 tctaacttct aggattgtct ctaaagctta gtttcttttt ctttatattt tcataataaa
     7981 agtttggttc tttaattttt aaacatgaca gaaaattttt tcttcgttat acttgtaata
     8041 gaatactatt ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat ttatactagt
     8101 ttatttgcag ctttgttttt agctgcttgt caatcaaata ccaatcaagg cacatctggg
     8161 aatacgtctt ccagtagcga acagcgctct tcacaggtat cggactcctc aacaaaagca
     8221 agcacatctt cgtctagcca gacaaaaagt tctagtgaag caagtactca gtcagatatc
     8281 aaagtgtctg cagctcgtgc gatcgaattg ttccaagata aatacccaga tactgcaatt
     8341 actagtttgg aattagatag tgattgggga tcttacttct acaagataga aggtgtcgat
     8401 gatcaaaaag aatacgaagc aagaattgat gctcaaaatg aaaaaatgga gacagagaat
     8461 ccagaaactc tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat
     8521 gttgataaat tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc
     8581 ggtacggcaa cagattggga tttggataaa gatttaggta ctacttattg ggaagtcaaa
     8641 gtaaaagacg gcaatcaaag tacatcagtc aagatcaata gccaaacagg cgaagtactg
     8701 tcaactgaac aagatgatta agaatttctt cggaaaacaa gagttgattt ttcttttagt
     8761 catgatacac tagacaaaat aacttgtttg aaggagcgat ttacttgagt ttagaaacga
     8821 cctttgccaa tcatatcttt gccaatcctt tgatgaatgc atctggtgtc cactgcatga
     8881 ctacccagga attagatgaa ctggctcatt cagaagcagg agcatttatc actaagagct
     8941 gtacgatcaa tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt cctttaggga
     9001 gtatcaattc tatgggactt ccaaacttag gtttttctta ttatctagag tatgctcttg
     9061 cgtatgaaaa agtgcaggaa aaccaaaatc aaccgttatt tttttccatt gccgggatga
     9121 gtgtccagga aaacttggaa atgcttgaga aaatagaaaa aagtgggttt aatgggatca
     9181 ctgaattaaa tttatcttgt cccaatgtgc ctggaaaacc acaactagct tacgattttg
     9241 aggccactta tgaaacactg aaagaagtat tcagcatctt ttctaagccg ctaggaatca
     9301 aattgcctcc ttattttgat tttgctcatt ttgatcagat ggctgatatc ttgaatcagt
     9361 ttccattgac ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat atcgatacag
     9421 aacaagaagc agtagtcatc aaaccaaaag aagggttcgg cggcattggc ggagagtata
     9481 tcaagccgac tgctttggcg aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc
     9541 aaatcattgg tacaggtggg attcggacag gacaagatgc atttgaacat ctgctatgtg
     9601 gcgcaagcat gttacagatt ggtaccgagc ttcataaaga agggccggaa attttttcta
     9661 gaatcataaa agaattaacg cagattatgt cagaaaaagg atatacgtca attgacgaat
     9721 ttaaaggcaa attacgcacc atttcatagc aaagagacga aggtcatgat tgatcttcgt
     9781 ctttttttat aaaaaacact cgtctgttca ttcctgtgca agcgtactca ttgtatgtta
     9841 gacgaacttg tgggaaaata acagaggcaa aaaggaggat aatatgaatg atgcaacaga
     9901 attagcaaaa gagcttaaag aaaaagaatt tagttttcaa gaactagtta cggatataca
     9961 gaaaaaaata aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga
    10021 gagtaattcc cacgagctat tgattcccca gtcagaaaaa gggccattgg ctggtatccc
    10081 atttccttta aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt caggatctcg
    10141 tttatttgaa acacaccgag cttctcatac atctaattat gtgcggcaag cagaagcgat
    10201 aggtctagtt ccttttggac aaaccaatgc gccagagttt ggtttcaaaa atattacaga
    10261 cccagtcatc tatggcccag caagaaaccc ttggaacctt gaatgtacac cgggcggatc
    10321 aagtggagga gctgctgctg cagtttcatc aggaatcgtt cctttagctg gtgctagtga
    10381 tggcggtggt tctattagaa tacccgcttc gttttgcgga ttgattgggc tgaaaccttc
    10441 gcgagggacg atgccagttg gtccatatgc ttggagagga tggcaaggag ctgctattga
    10501 tttcggcttg acagtttcta tgagagacac ggaggcttta ttttatggga tgagaagtat
    10561 ccattccggt gcgccatatc aagtatcgcc agttgagtgg caaacacatc caagaaaaaa
    10621 acggttgaag atagcttttt gtgttgattc accgatccac tcacagatta gtgaagaggc
    10681 agtacttgct gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg atttgacaga
    10741 gattccttac ccactagatg gtcgtaaact gatcacgtct tattatcaga tgaatggcgc
    10801 agaaacagca gcaatgatcc gctcgattga aacaggcatc cagcgagctg tcagaaaaga
    10861 agaacttgaa cccatttctt ggactttgtt ccaatatggt cagaagctct ctgccgctac
    10921 ttacatcgat tctctgcatg tatgggatga agcagcaatt gtcatggaaa acctacatca
    10981 agaatacgat ctattccttt caccaacgac tgcatttacc gcccctaaaa tcagcgaaga
    11041 tttgcaaagt gatgcgataa gagcacagat ggaacacatc agtgattgtt ctgaagtaga
    11101 aggtttagac gttgtttctt ccatgtttga ggagagcttg aagatcacac cttatacaca
    11161 gttggctaat ctgactggac aaccagcaat cagcttgcct actcatttga caaaagaaaa
    11221 catgcctcta ggaatccaat ttatggctgc cagaggaaga gaagatttgc tatttcaaat
    11281 cggctatcta tttgaagcaa atcatcagtt ccatttacca aatccatgga aatagcagaa
    11341 acaaggagaa gaagaatgaa gaaaaaaaga ttgtttgctc taataactag tgcctttttt
    11401 attttaagcg cttgcggtac agctgccgct aatcaggaag ataacagctg gagtaatgta
    11461 gaaaaaaaga aagaattagt agtagcgact tctggcacac tgtttcccgc atcttattat
    11521 aatgatcaaa atgaattagt tggttatgat gtagatttgg caaaagaggt ggctaaacgg
    11581 ttaaatgtca aaatctcctt taaagaatac aacgtggatg gacaaatcac ttctgtttca
    11641 aaaagagaat cagattttgc tgcgaacgat tttggtatca caaaagaaag agaaaaaaaa
    11701 ttctcccttt cagagacttt caaatattca tttgacagta tgatcgtacg aaaaaaagat
    11761 gattcaggaa tccactcact tgaagatctt aaagggaaaa aagcagctgg cgaacccaat
    11821 actagctata tgcgtttagc ggaaaaacac ggagctaaat tagtcacata tgacaatgcc
    11881 acgaatgatc agtatttaag cgatgtagca aatggtcgaa cagatgttat tctaaatgat
    11941 tattatctgc agaaaatggc cgtatctgct ttaccagata tccctgtaaa aatactggag
    12001 aacgtttatt tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa tgatgctctg
    12061 aaaaagaaaa tcgatggcgt attaaaagag atgaaagaag atgggacgac aaagaaaata
    12121 tctgaaaaat attttcaaac tgatgtttca gtcaaaccga aagaaacgat ggctgagtcg
    12181 gtgagtgagt gatgtacttt cccctaattg atttttccct catgtggact tcacttcctt
    12241 tcgtactgca aggcttggta tacacattag ggattggctt cgtgtcgttt cttttaggaa
    12301 atttggtagg gttgcttttg acagttttag gtcttttgga ttggctgcct ctgaatgtat
    12361 ttattcgttt ttatttatcg ttctttagag gcattcccgc actagtcctg ctgtttctgt
    12421 tatatttcgg gttaccttat caattaagtg ccttaacggc ttcagtcatc tgctttacga
    12481 ttaccagcag tgcatttatt ggtgaaatct atcgaggatc actagcaggc gtttcttcag
    12541 gtcagtggga cgctggttta gcttctggtt tttctttctt tggggtattg aaatatatcg
    12601 ttttgccgca agcttttcga atctcagtac cggcactcag taacgtagct atggatctag
    12661 tgaaaggtac ttcgttagca gcaacaatca cagtgccaga aatatttcaa aaagcaaaga
    12721 tcataggcgg tcggacattc gattattttt ctctctatat tttagttgca ctgatttatt
    12781 gggtcatttg tatcgggatc gaaaaagtgc aacagtattt agaaaaacgt tctatccatc
    12841 agtcaatctg aaaaatagaa atggtgctag cgtggctggc accatttcta ttttttgttt
    12901 cttgtatatt ctgcctagta ttttaaaacc atttcatgta aaatgtaaat cgaaaatatg
    12961 ttcggaggta gaaaacatga agaccccgct aaccatcgaa atagaatcct cgttatacca
    13021 atattgcatc gagcaaggcg gcttggttgt agaagaagtc acgatgcctg atgaccaagg
    13081 aattgtagat acgcttagct gcttgacaaa atttgatgga agccgagaat ggcgatgcta
    13141 tgagctgaaa gtgacaaaag cagattttcg ctctacagcc aaactgtcat ttgttggaca
    13201 ttataattat tttgttttac caagtaaact atatgaagaa gtaaaaactg aaatccctac
    13261 aggaataggt gttttagtgt atcgtccgta cacggttgaa gaggagatgc ctgcctctgg
    13321 tacatttctg attgcgaaaa aagcagcaaa gaaagagctt gctgtaccgg aagaagcttt
    13381 gacaaaccgc ttcatggctt ctttattcag ggaagtaaga aaagcaaagc aaatggagcg
    13441 aggcgtaaaa tattttccaa aagatcaatt gtacaaagaa ttgaaaagac gttcagaaaa
    13501 tcaagatcca ttttctaacg aacgatatta tgaacggttt ttagaggaaa tagctagtca
    13561 ggcaatcaat gagttgcaag aagaagttat cgcgcttcag catgattatg aatatttgag
    13621 gcaacaacag cagatccgtc ggttgcctac agagccattg gagtgaaaaa tatgtactat
    13681 ccttatttaa gaggaaaaca atttgaccta ttagcattaa aagaagcact gtcgcgagga
    13741 ttgctctcca ataaaatcca accagtgatc gaaccagtac gtgattccgc tacgcttaaa
    13801 aatgtgatcg aattatttca aaaaaagcac catccaatcg ctgtgatcca aaatccccaa
    13861 gtaggacaat tcaaattatt cgatcagcat gtgcacagtt ggacagtcaa agaaaatagc
    13921 tctgtcgtcc ctgcacagat cttaacgcca gaaaatctca gtgaagtact ggattcacct
    13981 ccagcgtttc ttgttttcga tggacagcac tacccaaaag atacagaggt gtggaaacag
    14041 ttggcaggag ttgattcgaa atttttgata ccagatacta gtcgctttcg catctggctg
    14101 cccgaaaaca aaattgtgat ccgtgactct tttcaaacga gaaagcatgt tgaaagttat
    14161 gcagataaaa cagatgactt ttttagtgat gactatcttt ttttccacgc agacggatat
    14221 attggatttt ctgatttcac gatcgaaggt agccgttatt tcgataaagg gttccctagc
    14281 cgagcgcttg ctatccatct aacgtatatt gatgcttacg gcaatatacg cgtgaaacat
    14341 tttgtctctg acagtaacga ctctgctaaa gaccaagcat tgaagtttct tgaagcagga
    14401 gataaaatga aaaaatggat catgcgtcac catcatcaac tcttgattac ttcaggaatg
    14461 attgaattat tggctcttta ccagcaacaa aaattccctg gactgggagt gttgaaaaag
    14521 tggtcactta tgcatcattt ggaacttatt agtcacctgc tagatcatcc aaaagattgg
    14581 cttaaatatt attcaaaagt tcaggaacta tatgaagtga tccaatgatt agataatgta
    14641 aatgaaaaag gaatagaaaa gaggaaactg gatgtatatt cggcaaacaa ggaaagaaga
    14701 agtagaagaa gtggtagaaa tcattaatgc cgcagcggat ttcattgctt cgcaaggctc
    14761 tcctcaatgg caaaacgggc agaaaccaac aaaagaaacg attttgaacg atattgaaag
    14821 aaaagaaagc tatgtattaa tagtggaaga tcaaattgct ggaacagctg cattagtctc
    14881 aggcgtggat cccgtttaca caaaaataga tggtagttgg aaagaatcaa ctggtcctta
    14941 tttatcgatt catcgtgtcg ctttagcaga aagtactcga ggaaaaaaac tagcaaaacc
    15001 gttcttagaa catttgttga cgattgcagc cagcaaaaat atccaagata tccgtatcga
    15061 tacttatcca gctaatgaac cgatggaaaa gaccatctat tctgctggat tcacttatca
    15121 agggatgatt gaatttcctt ttgaacacgg cgaaagaaaa gcatatcaaa taatcatcaa
    15181 ttgactataa aatcatctga gcaaagataa agacattaac actactgaaa aaaacaatca
    15241 tatgatagcc aagaggttgt aacagaagtg gccagcttca aaaaacaaga aagaatttcc
    15301 gaaaatgctt ctcaaatttt tgtggaattt tagcgtattt tacgaaatag ctgctttctg
    15361 aaactccctt catcgagagg tcgtgacata gcttttgtca cgatctctcg gcttatccta
    15421 cgagtattat tcgatacatt tatatcaata atcctttttt tattttatct gcagcttctt
    15481 ggaacgctcc atcgtttgac acgactaaaa gtgaggggat agctttcaaa cgtttcaact
    15541 gtgctaaatc ttctacataa accgccagtc tggctgaaat ctgttcagat gaatctccac
    15601 gttttttcag gcgcgattgc aatgtttctt tagagatcgt aataaagata ggacggacta
    15661 ccgtcccgaa atactcatat aaatgccaaa aaccatttgc tgtcaaaacg gcgtaacaat
    15721 ttcctactgt cagtttcttt ttaatttctg ctttactgga accatattta tgttgagcat
    15781 attggtccca ctctgcaaac tcatttccct tgatcatctg attgaatgta ttgtcagaaa
    15841 caaaataata atcctcttga tctttttccc caggtcttgg tgatcgattc gtgtaagtaa
    15901 tgatcttttg ttccggctga aaaatagctt ctgcgagtga agtttttcca gaaccagaag
    15961 gaccgataaa tacatagcaa taattcattg tcatttctcc tttgatgatt gaacaagtga
    16021 aaaagctttg gaaaatattt ccaaagcttt taaaagtaac tttgcttttt tgtattttat
    16081 accagcaagc cagtcacaat agcacttaaa aagctgacta aagtcgctgt tagcaaaatt
    16141 ttcaatccgt gttttgcgac aagatttcct ttttcttcat ttaatccctt catcgcacca
    16201 gaaatgatcc cgatagaaga gaagttggca aatgaaacga ggaagacaga tataattgct
    16261 gttgtacgct cagtaaaatg gaaattacca tttgctaatt ctgtcatagc aacaaattca
    16321 tttgtaacca gtttagtcgc catgatacta ccggcatcca ctgcttcttt ccatgggatt
    16381 ccgctgatga aggcaagagg ggcaaagatg taacctagga tctcttggaa agtgatgccg
    16441 aatatcccgt ggaagatagc attgatcatg gcaatcaaag caacaaaacc gataagcata
    16501 gcagcaaccg tgatcgccac atggaaacca tcaagaatgt attctcccaa catttggaaa
    16561 aatgtctgtt ctttgttttc ttcaatgatc agctcatctt ctttttcttc taatacatag
    16621 ggatttacaa ttgaagccag aatataaccg ccaaataagt tcaaaacaag ggcagtgatc
    16681 acatatttag aatcgatcag tgccatataa gatccgacga tcgacataga aactgtagac
    16741 atagccgata cactcatggt gaacaaacgt ttctcagata ggaagggtaa ttcctttttt
    16801 atggaaataa aaacttccga ttgtcctaaa atagcagaag ctactccatt ataagattcc
    16861 aatttgccca tcccgttgat tttacttaat cctagaccaa cagctttcat aaataatgga
    16921 agaatcttga tatagcgtag aataccgata atagcagaaa taaatacaat tggcatcaat
    16981 acggaaagaa agaaaggtgc acttccttta tttgctacac cggcaaaaac aaagtcaaca
    17041 ccttgcccag caaaagcaag aagatgatca aacatttttg ccaatacaga aacaaccgct
    17101 gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac gaacatcaca
    17161 attagtggtt tcaatttgat tgcttttttg ttactgctga tcaaaaaagc aagccctaat
    17221 gtgacaaata aaccaataat acctaataaa taactcaaat aatcactcct attccaaatt
    17281 ccgttttatt ttacctgttt tgcaaataaa atactaatct ttagtgaaat tttcattact
    17341 tttttagagt gaaacgcatt cattttccat tagaaaataa agcgaacaaa ataaaaggtt
    17401 taaaaagtgt aacgtcttag taaagtctat aaaaaaacga aactatcgat taagctaata
    17461 gtttcgtttt caacatttat aagtaaactt tttctattgg taatatagat tttcggaagg
    17521 gtagacaggt gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt catcattttt
    17581 attgaccatc acagtagagt gggcttgtgc tccctcaagt tcagctagtt tatcataagc
    17641 aacttgagcg attggattcg ttactgcact gattgataaa gcgatcaaga cttcattgat
    17701 gcttagtgca tccaaagagg aatgaagtgt ctttgatttc aatccttgta ttgtttcaag
    17761 aactaatggt gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt ttactgcatt
    17821 taagataact gccgctgacg catccatcaa tgaacttgta cgcccggtaa tgatgacttc
    17881 atctggtaat tcgattgcaa tcacagcaga aggatctttt gtaggacttt gcgcttgaat
    17941 cttatctgca tagtcatggg cagggactac cacgtttcgg tcttcaggac gtaaaccgac
    18001 ttcttccata atcagtttga ttcgattaac tgcttcttcg tcgatcaatc cttttttgaa
    18061 gtcgcattct gtttggaaat aacgacggat gatttcttgt ttagaggctt cttggacaac
    18121 ggtatcatcc acgatcccaa atcctacacg attcacaccc atgtcagtag gagattgata
    18181 aacagattct ttaccagtaa ttttttcaat aatacgtttg attacaggaa atgtctcaac
    18241 atcccgatta tagttcacag ctactttatt gtaggcatca aagtggaagg aatcgatcat
    18301 attgacatct tttagatcga ctgtggctgc ttcataagca atgttcagcg gatgtttcaa
    18361 cggaacattc catactggga aagtttcgaa cttggagtaa ccagctgcat gtcctttttg
    18421 gctttcatga tacagttggt tcaagcaagt agcgagcttt ccacttcctg ggccaggagc
    18481 tgtcactaca acgattggtt tagttgtttc aatatactca ttttttccaa aaccatcttc
    18541 tgagacgatt ttttctacgt tgactggata gtcttcgatt tctgagtgtt tatagacctt
    18601 gatgtttcgt ctttctagtt tattgataaa gactttcgtg gcaggctgtc cactatagcg
    18661 agtgataacg acactgttga ttaataagcc ataaccacgt aattcatcga ttaaccgcaa
    18721 gatatccata tcataagtaa tcccataatc accacggatc ttattacgtt caatatctcc
    18781 agcatagacg caaatcagta tttcagcttg atctttcaat ttttgcagca atttgatttt
    18841 cgcatcttct tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa ctaacttccc
    18901 accaaattct aaatataatt tgtcatagtg gttcactcgt tccaaaatat acgctgactg
    18961 ctcttcaata tatttttctg aatcaaaacc aatttttttc actaggttcc ctccttaagt
    19021 actaaagcat tttttcattt ttttgaggat tcgtcaactg gaagagcgaa attcctcaaa
    19081 aaaacaaaga aaatggttta aaccaacttg tctagatagt tattcattgg tatgatagtt
    19141 tttgataagg aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca tataaacact
    19201 atatccgttc gatttttacc ggttatgaaa gaatgcttga agatcccgaa ttggaacaat
    19261 tgacgtttac ttatctaaac gaagaaacgc agctaactcg tgatgagcat cagcgcattc
    19321 atttcacaac aagagatctg cctagtaaat aaaaaataag ataaaggtca tcggattcga
    19381 ttctgtccta tcagatggaa agactagttg atcgaaaaaa aggctgtacc aaattttggt
    19441 gcagcctgtt ttatggagca ttgcccgctc ttttttataa caatttttta cagaaaatgg
    19501 tactattgaa gtattaataa gtgaaaaaca gatgagtaag aggaggcaag tagtatgaaa
    19561 gagccagata caaatgcaaa ttatgagcca gacgaactag aggagacgaa cgcggatcag
    19621 gatacaggac aagaatcgtc ttctttttac gatatcaaag aaaaagcaag catgaatctt
    19681 tcccaattat tagaagactt acaggctttt gaaaaagctg tccgcgaaga agatatccct
    19741 gagatctacc gtttatacaa tggccgattg aatgaagaat taaaaagaag ctcaaatgaa
    19801 aatcacgaga tcgatcaatt gctgatgcgt aagatccatg atcgtttttt acaggaattt
    19861 ccttttatgg aacaagtaga aaacatctcg ccaacgatga gctattacaa aatcggcact
    19921 tattaccatg atcggcccac ggtcggtatt gatgcaagtc tgccagaaat ttttgttttg
    19981 cctaagatcg atgaagagtg ggagaaatat tcccaaccgg agtcaacgga ctatgacaaa
    20041 aaaatcaatg agctagacgc aaaggtgatc actgctcaga cagaaattga acgtttagat
    20101 gaacaaataa aagaaatcaa tcgacaaatg actgatttga acgacaataa aggattcttg
    20161 aatcgtaaaa aaatcgagga agaaattcaa gaattagaga agaagaagca agtactatct
    20221 aatgaaaaat taggctggct tccttatatt gagacgcctg aaacaatcca gcagcaaaaa
    20281 gaagcattga agcaagaagc aagagcagac cagcttcgtg cagccatcgt agaaaaagaa
    20341 caaaggcaaa tcagacgcta tttcggtagc aaagagggat tcggtcaagc aatccatgaa
    20401 ttcttgatga attatcttgg caatgagaat cctaataatc aatctaatga aggaggttcc
    20461 gagtatgaat gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa cagaagatca
    20521 tgaagaaacg aacacttcat tggaagaaca aacaagtaat cctctggatg acacagaaga
    20581 aatccctatg ctagaggaaa caaaaataca tgaaaaagaa aaagtaactc cgacgaatac
    20641 gaatactgca gaaaacaccc atgcttacga aataactgaa aatcccgggt atttagaagg
    20701 aaaagagcaa gatcatcaag atatcgtcag tttgattgaa acagcggaag cagaattagc
    20761 ggatttacgt atgagaaaag atttgatcga atcagatttt ccacaattat tagaatcata
    20821 tgatcgtttt atattgaatg aagaaccaat ctccacgatc ggaaagacaa gcctgatcga
    20881 atacttcacg tatgaattac tgaaaccgtt gaatgatatt ggtctggaac ggtcaaattc
    20941 agcttatgac acatggaaaa caagacattt ttcgatcaat tatacgttga atggacaaaa
    21001 cgaattagtc ttttcctttg tattaccgac catcgatcaa cgataccaag tggaatcgat
    21061 gagtctccta aacgtatcac cagagacaat ggaaatcaat gtccaagatg accgggtgct
    21121 ttctttgatc cgacactggt cagtggatcg agtattctct gcagggcaga tcacgatatt
    21181 caatcacaaa ttgaatcaat tattagccca tgcaagaaca cttggtttta ccgttaatca
    21241 gacattacta gacaatacga agccacttcg tttgaatctt cagagcgaat ttgaattgac
    21301 gaatgaagta ttggatgata tttttatcgt ggcaatgaaa aatccatcct acgatatgga
    21361 aaagatcaac gaaactactt atcaagtacg attagataaa ggacagtctt taacgataac
    21421 aacaaacgaa aaagatcaaa cagtcttgtc gatctcatct ggagactacc cacgatctgt
    21481 cattgatttc tttatcaatt acgaattctt ggtcccatta atggtgagaa agtcctgatt
    21541 ggataagttg gattaccaga tgtcattcag ataattctgt tccaaaggac ttgcggcagt
    21601 tatccagaat aattgtcaca agtcttttct gtatgtacta aatggacgtg aataacttgc
    21661 tgatgaagga gggatttcat taggaatgtc cacaaataaa atagcacagc ttagcttatt
    21721 gtctgctgct tgtgtagcag gtaggatcgc ttttcaattt attccgaatt tccagccaat
    21781 gactgctatt tttttattta tagtacttta cttaagctta aaagatgcgt taattgtcat
    21841 gagtctttct attgcgatca gcagttttta ttttggtgtt ggaccatggg tcatcggcca
    21901 atggatcagc tatactgttg ttctgagttt gttttcaatc gtctgcttga gaaaaacagt
    21961 gcaaaggaat ctatggttaa agggagtctg tttttttctg gcgggcatgg tttacggaat
    22021 aggtatgacc gtttttgata cgctgttata tcgtttgcct caaccttgga tctattacct
    22081 tcaaggggtg tcgtttgatt tgatgcacag tataggcaat gttgtgttct ttcttgtttt
    22141 tctgccagtc gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa aaaataattt
    22201 atagtttgct tgcaatgagt gtcagtatac tcattttgac aagttgcagt acaacctata
    22261 cacaatcggc ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca acaatcacgt
    22321 tacaagaaaa tggtcaaaac ttttcggaaa aagaagtggt cttcaaaaaa ggcgatgatt
    22381 tactgagtat tttgaaacga aattttgaag tcaaagaaga taacggcttc atcacttcgc
    22441 ttgaagggca ctcacaggat gagaaaaatc aactctattg gatgtttact gtggacggga
    22501 aaagtgctac tactggtgcc aaagagatca aattgaatga tggacaatcg gtcattttca
    22561 atttgtctaa actataagtt tcataaaaaa ctgttaaagg gcgaatattg ccataattca
    22621 gtgagttcag ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa agcggaatgt
    22681 tttccgtaat tgctgctcta ggggctaata ttctagttgc tatcagcaaa tttgtcggct
    22741 atgcaatcag tggcagtgcc gccatgctaa acgaaagcat ccacagtatc gttgattgca
    22801 gcaatcagat tttgctgtta ttcggtgata aacaagccaa taaaggacag agcgagttgc
    22861 atcaatttgg tgaaggacga gcaaaatact ttttcagtac aatcgtcgca atgatgcttt
    22921 tcttcggcgg tggggcactt ggggtgatgg aagcttttga aaagttgacg catccatccc
    22981 atgaagtggg taatccgatc attgttatag caatactgtt atttggtttg gtaatcgaag
    23041 gaagttcgtt acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa aaactttcca
    23101 cgatgcgctt tttgcgcgaa agtcgacaca gtgaaatact gattattttt acggaagact
    23161 tgtgtgctgt aatcggtttg gtcttggctt tagcaggaac aatattgact tatgcaaccg
    23221 ggattgctgc atttgatgcg atcagcggat tgctgatcgg tttcttgctg atgggagccg
    23281 ccattttcct agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg acagcaagcg
    23341 atttatcaaa aatcaagtta gcttttgaac gtcctgagat cagtcggctg atcgatataa
    23401 aaacagttca tttaagtcca acggagattt tagttgcagc aaaagcagat atcgttggta
    23461 acaaagagaa tgaagcttac tcggtcatca atgatattga aaaaaatatg cgtcagtctc
    23521 ttcctgacaa gaagatgtat atatacattg aaacagataa atatgaccca aattattctc
    23581 gtaaataaaa cgtttattca tgaattgaag caaaaatgag aaaaatataa tcttgttttt
    23641 aaataaaacg gctacaattt agcgaattta tgttacaatg agtccgctgt ttatttgttg
    23701 taattaaagg agatatttta atgaacaaaa agaaaagtgc gccatggctt caagctttgc
    23761 atgcagtgat gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt
    23821 tgcaaaaagt tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg
    23881 gtggcgcgca gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat
    23941 tattgatggt tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt
    24001 ttatgaaaaa agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa
    24061 actatgcggt gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt
    24121 tatatgtcaa ttggttctca atgactgcat gggccggtag caatatgatc ggtaattttt
    24181 tcggctcggt gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca
    24241 ttttcatgtt tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag
    24301 gcgtattagg ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa
    24361 cagtcattgc ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta
    24421 agaaactaag agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg
    24481 aggccaatca ccatgactaa actatatttg ctacttttag ttgcttgtct gttcatcgtg
    24541 gcatatatcc cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg
    24601 ttcagtgact ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat
    24661 gttttcatta ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt
    24721 ctagtagcag gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt
    24781 gcgtctgtct ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg
    24841 cgacataagt tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg
    24901 caagtttatt cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta
    24961 ttttaggaat tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt
    25021 ttattttatg aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca
    25081 ttttggctaa cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc
    25141 tggctaagta tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt
    25201 ggctatcttc cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt
    25261 gcaaaaaaag tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt
    25321 agaagtacga tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta
    25381 tttgctcctt tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct
    25441 atcagaagtt tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat
    25501 ttccaaggaa aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg
    25561 cgagtgatcg ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata
    25621 ttagaagctg tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc
    25681 catatgtata tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc
    25741 agccgttatt tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta
    25801 ccgtttattt ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg
    25861 aaaccactgc ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc
    25921 agccgtgctt ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca
    25981 gtagctataa gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc
    26041 gaaaaaacag taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact
    26101 ggaatgtctt tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt
    26161 gtcggatatt tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccta
    26221 tctacgatgc ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca
    26281 attatcctga aagtcatctt ccaagtaatc ggattaggat tagttggcgg atacgggatg
    26341 agcttatcaa atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc
    26401 aaagaatacc gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc
    26461 tccaccctga ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg
    26521 tcaatggaat caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc
    26581 ttagtatttt tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa
    26641 catcatagca gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg
    26701 gcaactcgta tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat
    26761 cttacttatg atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac
    26821 atgaaacttg cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat
    26881 cccgacttat tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga
    26941 atcgatatcg aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact
    27001 acttcacaac ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg
    27061 tatgacacga ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta
    27121 tttgcgatga aagatgacat cagcggagta acggtcattc cttatgactc taagatcact
    27181 agtatgccga tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc
    27241 cttgatgaaa tacttgcaca tatcgatcga atccgagata atacctatgc ttatctgatt
    27301 gtagatgatt tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt
    27361 ggcagcttat taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc
    27421 gaaaaaatcc gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga
    27481 aataacgaaa tacaaccgcc agtcaaactt tacgtgatac atgcaaataa tttagaagta
    27541 gcgattgcgg agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt
    27601 catttcggac cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca
    27661 gcgcaataat tccttttatt caaaaaacat accaaaaata
//
LOCUS       DARBFD010000028        26434 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000028 DARBFD010000000
VERSION     DARBFD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.028"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..2198
                     /locus_tag="SCK08_001166"
     CDS             138..2198
                     /locus_tag="SCK08_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEG1201071.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2281..2850
                     /locus_tag="SCK08_001167"
     CDS             2281..2850
                     /locus_tag="SCK08_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1201072.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3025..3831)
                     /locus_tag="SCK08_001168"
     CDS             complement(3025..3831)
                     /locus_tag="SCK08_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201073.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3969..5483
                     /locus_tag="SCK08_001169"
     CDS             3969..5483
                     /locus_tag="SCK08_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEG1201074.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5886..6869)
                     /locus_tag="SCK08_001170"
     CDS             complement(5886..6869)
                     /locus_tag="SCK08_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEG1201075.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6874..7521)
                     /locus_tag="SCK08_001171"
     CDS             complement(6874..7521)
                     /locus_tag="SCK08_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1201076.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7606..8238)
                     /locus_tag="SCK08_001172"
     CDS             complement(7606..8238)
                     /locus_tag="SCK08_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1201077.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8254..8805)
                     /gene="coaC"
                     /locus_tag="SCK08_001173"
     CDS             complement(8254..8805)
                     /gene="coaC"
                     /locus_tag="SCK08_001173"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEG1201078.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8802..9539)
                     /locus_tag="SCK08_001174"
     CDS             complement(8802..9539)
                     /locus_tag="SCK08_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEG1201079.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9536..10525)
                     /locus_tag="SCK08_001175"
     CDS             complement(9536..10525)
                     /locus_tag="SCK08_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1201080.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10783..11325
                     /locus_tag="SCK08_001176"
     CDS             10783..11325
                     /locus_tag="SCK08_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1201081.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11342..12427
                     /locus_tag="SCK08_001177"
     CDS             11342..12427
                     /locus_tag="SCK08_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201082.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12439..13245
                     /locus_tag="SCK08_001178"
     CDS             12439..13245
                     /locus_tag="SCK08_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201083.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13246..14073
                     /locus_tag="SCK08_001179"
     CDS             13246..14073
                     /locus_tag="SCK08_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201084.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14070..15143
                     /locus_tag="SCK08_001180"
     CDS             14070..15143
                     /locus_tag="SCK08_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1201085.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15571..18267
                     /locus_tag="SCK08_001181"
     CDS             15571..18267
                     /locus_tag="SCK08_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1201086.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18344..18832
                     /locus_tag="SCK08_001182"
     CDS             18344..18832
                     /locus_tag="SCK08_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1201087.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18844..19338
                     /locus_tag="SCK08_001183"
     CDS             18844..19338
                     /locus_tag="SCK08_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1201088.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19359..20168
                     /locus_tag="SCK08_001184"
     CDS             19359..20168
                     /locus_tag="SCK08_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1201089.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20165..20986
                     /locus_tag="SCK08_001185"
     CDS             20165..20986
                     /locus_tag="SCK08_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1201090.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21037..23136
                     /locus_tag="SCK08_001186"
     CDS             21037..23136
                     /locus_tag="SCK08_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEG1201091.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23133..24251
                     /locus_tag="SCK08_001187"
     CDS             23133..24251
                     /locus_tag="SCK08_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1201092.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24290..25168
                     /locus_tag="SCK08_001188"
     CDS             24290..25168
                     /locus_tag="SCK08_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1201093.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25359..25511
                     /locus_tag="SCK08_001189"
     CDS             25359..25511
                     /locus_tag="SCK08_001189"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1201094.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8375 a         4564 c         5562 g         7933 t
ORIGIN      
        1 ttttcttgaa acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga
       61 ttttatttat ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta
      121 gggaggacta agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt
      181 gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca
      241 cgatgctgat cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac
      301 aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta
      361 ctcatacttt cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata
      421 aagtcagtta tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga
      481 catcggaaac cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa
      541 aaggtgatca gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg
      601 acggtggaag cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa
      661 agaaaatcga tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga
      721 agaacgcaga tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg
      781 tggatacgac taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt
      841 taaaaacaat gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag
      901 gaaaagatct tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg
      961 taacaaaaag cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag
     1021 atactacaac tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa
     1081 atgtggcaga aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca
     1141 tcactcattt tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta
     1201 gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata
     1261 tagatttatc gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag
     1321 caaacggatt agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac
     1381 tgatgaataa taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag
     1441 acaatcgttc attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag
     1501 aagagttgat ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat
     1561 cggttacaat caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt
     1621 ttacgattat gtatagttat catgacccgt attcactcca agaactccat gctcagtcac
     1681 tattacatgt tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacgagaag
     1741 aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa
     1801 aacaaacaag aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa
     1861 cgataaggca agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag
     1921 agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga
     1981 aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg
     2041 atgagcatct gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag
     2101 gtattccttc ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg
     2161 ctaatcgaaa tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag
     2221 tcaaataagt agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa
     2281 atgttagata aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat
     2341 gttccaatca cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa
     2401 tctgcttggg cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt
     2461 gagtattttc aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt
     2521 gtagagacaa aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga
     2581 gagcaagtgt atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg
     2641 ctcgcaaaag cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga
     2701 ccaatctatc ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg
     2761 ggttttactt ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt
     2821 atgcaggttt taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg
     2881 gataaaaaac gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata
     2941 actaatggca tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc
     3001 ccttctcttg ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat
     3061 gaacgtaaat ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac
     3121 tgctccaata aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta
     3181 accattttgg atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt
     3241 ccgagtgata tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt
     3301 gatttgctgg atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa
     3361 ttccacaaac tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag
     3421 taataaagga gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa
     3481 gccgacaccc ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa
     3541 atagccataa aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa
     3601 aatgatttct agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat
     3661 ttcaaataaa attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac
     3721 ctgatccact agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat
     3781 tctacctgca gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact
     3841 ccttcatgta tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta
     3901 aagaaaaaca gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg
     3961 ggatacaaat gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga
     4021 gcgcatgcgg cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg
     4081 ccgaagtatc atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat
     4141 cttatgatgt cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa
     4201 aagatgctgg gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat
     4261 ctaaatcatc aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca
     4321 aagactcgaa tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata
     4381 aagaaatgct tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa
     4441 acggtatcaa actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc
     4501 gtccgtctga tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc
     4561 atgagcggga aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg
     4621 aaacggtcaa tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa
     4681 aagcaatcgt agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc
     4741 gtcccgattt agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca
     4801 ccatgattga aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa
     4861 ctgtgcagca ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct
     4921 tggtcgatca agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg
     4981 cagcaattac agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg
     5041 atcgtgccac agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa
     5101 tggaagaatt agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg
     5161 gatggaatac ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg
     5221 aacatgactt atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca
     5281 cgatgggcgg tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa
     5341 ttagggggtt atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg
     5401 gtggaaattc agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc
     5461 gatttgcttc ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg
     5521 ttttgagcta ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa
     5581 agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac
     5641 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg gaaaaagagc
     5701 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
     5761 cagaagaaat tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt
     5821 gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt ttgatcagca
     5881 tgattttatt cagttatctt caaaatgatt tttccgatgt ttttgtttgc ttccatatat
     5941 tcatgcgctt gtttgactgc gctgaattgg aatgtacggt caatgattgg ccgaatcttg
     6001 ccattttcta gaaaaggccc agctttttgc ataaaagttt gtgcgagctc tgctttatac
     6061 tgatcacttc taggtgtcaa taatgtacca agcagttgga cacgtttccc aatcaataca
     6121 ccaaggtcta tctcaggcac gatggtccca cctagcatac caatcaagac ccaacgtcca
     6181 tcaacggcta tagaagctaa attcttttcc cagtaagaag cgccgatgaa atccaaaata
     6241 acatcgactc cccgaccttc agtgattttt cgtacctctt cttgaaagtc ttgtttttta
     6301 tagttgatca attcatctgc accaagttct ttacaaaagg cgagtttttc ttctgatcct
     6361 gcagtaacga tgattttcgc gtgagaaagc tgtctagcta attggatcgc tgctgttccc
     6421 acaccacttg ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt tccaagccag
     6481 taaagtgttt gataagcagt caaaaaaact tctgagattc cagcagcaga aacatagtct
     6541 agcgtatcag ggagaaggat tgcacgatcg gcaggcatca cagcatattc ggcataacca
     6601 cctagattga ctaatccgta tacttttgtc ccaggagtaa aagtggagat atcactttta
     6661 ttctcaacta cctctccagc tacttcgact cccaaaatag ggtagggagg tttcaaagag
     6721 actgattctc ttttcataat atctgttcga ttgacagctg ctgtatggac tttgatcagt
     6781 aattcgcctt gttttggttt aggaacaggc acttcaattt gttctaatac atctacagaa
     6841 ccaggatgct tgattgaaat ggcacgcatg gatctattcc tcctttgccc attcttggaa
     6901 tgctgtattc aactctttgc taccttcaga aagtactaga gtcgtacgag aaatatccgt
     6961 ttcttccact agagcggcaa gtacagcagc aactgtagag cgagtgatcg tttttgatgc
     7021 cttctcatta ggcttagtta attgaatgga agtaacttct tcatcgttag tcaatgtgac
     7081 tggttgaaga atgacaaaat ctaagttagt tcgtttcagc cattcatctg cataatgttt
     7141 agtaatataa taatctgtca tggactcagg ccattttgct ggttcatctg cataaacagc
     7201 actgaccatc agatagcgag agacatgcgc attttcagct gcgatcactg ttttgattgc
     7261 gccatccaag tctacttgca gaagattctt tccttgagag ccggcagcaa aaatcaatac
     7321 atcgatgcct ttgaatgctt cggtcatttt ttccacggac caagttaaat caaaagagac
     7381 atacgaaaca ttctcttttt ttactaatgt ttgggaagca acatctctca cccctgcaaa
     7441 aatttcatgg gaacttggag ctaaatcttt tatcaggtgt cggccaattt gtccgtttgc
     7501 acctacaaca aaaattttca tttggatcat cctttcgacg tcaaatcttt ccattcaata
     7561 gtaacaccaa aagagaaaaa aatcagagat cggctacgat gactatcagt tagtaagaga
     7621 agggtgcttg aagtatctta acaagacatt gcatactgct gcagtcgcaa gacctgcgac
     7681 gattccttca ggtataccac ttgtaaaaat aaccgctaga atagctgaat aaacagcatt
     7741 taaatttgtt ccgatgactt tggcataatc ttgctggaat aaaaagtaga tcatattcat
     7801 cactaaaatc gtattggtca atgaaccgga aagtccagct aagaataaag aaacggaatc
     7861 tgctttttgt ttcattagtt tgcgcagacc tttataaacg aaataaggaa ccaccccaat
     7921 caagatgcga ggaataaacg cgatcagcaa ggctttccag cttccatgtt ctgttccgat
     7981 gactgggata aacggagaaa agacaaaaga caaaggtgtt tggataactg tgctacggat
     8041 catgctgatc agtccaaatg taccgccaag aaaagcacct agtcttggtc ctaaaacgat
     8101 cgaagcaatg attacaggaa tatgcatggt tgttgcattg attggaccga tagggataaa
     8161 acctaaaggc gtaacagcta ataaaatcaa aatagctaaa aacatcgcag ttaatgtaaa
     8221 gttttttgta tttttcataa gtactccttt tttctaagca tttctttcat taagagtcag
     8281 catgacctgt tcgatgatcg tctgaatatc agctaaggca ccttttccaa aatctccaca
     8341 agctaaaaga gattctcttg gttcaatttc ctgatagccg acagatctca atgtttccag
     8401 attacgctga gtgatcgggt tttgatacat attcgtgttc atggcagggg caatcaattt
     8461 tggtgttccc actggaagcg ccattgcgac tgttgaaaga agatcgtctg caatgccgtt
     8521 tgctagcttg ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt cagcttgttt
     8581 tgctagttcg atatgattga ttacctgcgg atttgattct tgcatgacgt ctgtatggac
     8641 tgcgcgcttc gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc tgcttttaga
     8701 cataatcaca tctacctggg cacctaattt gacgaattga cttgtaagat cagcgctttt
     8761 gtaagcagaa atacttccag taacgcctag taagatcttt ttcattttga ttcctccagt
     8821 ctaattttct cagtaatcaa tgcagcaatc tcagatttac tttgagcttc ttccactgtt
     8881 ccatctttag ataagagata gccgtggtgt gtctcgccat ggatttccgt caaatcattt
     8941 gcgaaaataa aatcagtttt gttatttatc agactatttt ttgcgactgc cagaagagat
     9001 tcctttggta catctactaa tagtttgaaa ccaatgattt ttgcttttgg ctgccattca
     9061 cgtagataag aaatgatttt tggtgttttc ttcaaaacga gcacaagatg ctcggtgtta
     9121 gaagagatct ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca attgtccagt
     9181 tcttcttgcg ttgaagaagt gggtaggttt tttgccagct gttcttctga aaaactaaaa
     9241 gcaggagtaa aatcactgac tgccatactg tggataatcg catcgtagtg ttcttttttc
     9301 gttagttctt ctaattgtaa aaataagtcc agtgcagatt cgatcgtata aatcgttata
     9361 ttggagtgtc ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat tgtggtatcc
     9421 ggattggcag ctaaataatc agctaatgct tgccctaatc gtcctgtcga atgatttgta
     9481 atagcacgta cttgatcgat tttttcagtc gttcctccag ctgtgattag aatcttcatt
     9541 tatggtcacc tacatttact agtgttcttc cttggtgtgt tccagcaagt agtttagatg
     9601 ctgtttcagg caattgatcc agagtaatct cttggacgac cagttcatct gcaatctgca
     9661 aatccgccat ctgttgccat aaagaaagac gttgatctat tggtacattg acagaatcaa
     9721 ttccaatcaa ctggatgttt cgcagaataa aaggcaagac agtcgcgttt agtttgattc
     9781 ccccagcatt gccacataaa gctactgcac cgttataaga gatcaatgga agaagactgc
     9841 ttagctgctc gccacctact gtgtcgatga catagtcgat ttgctgtttt cctaaagctt
     9901 ttgttgtctc tggcagaaat tcatcaggtc gtacgacttg caaagcacct agacttttta
     9961 gccatgtgac agactcttct tttctggaaa aggcaatgat tgactggaat cctagtttat
    10021 gtaacagggc tatcgctgta ctgcctacac cgccagaagc accagtaacg acaacttttt
    10081 tatcggaggt cataccttga cggattaaag cgttcacaca caacatagca gtaaaccctg
    10141 ctgtaccaag gatcatcgct tgacgcagcg tcatattttt tggtagagga actaaccagt
    10201 ctcctggtac tttttgatat tggctataac cgcctggatg ggtgacgcca agcccaaaac
    10261 cagtagcaag tacaagatct tcttttgaaa aacgtggatt gttactttgg acgacagttc
    10321 cggataaatc aatccctgga gtcatcggat atgttcgtat cacctgacca gactcgctca
    10381 ttgccaatgc atctttataa ttgacatcag aataggcaat ttttaccaag acttcatttt
    10441 cagccaactc agtaaatggc agatcactga ttgctgtatg gaaaacaggt tcctttatta
    10501 cctgaaaaga tcgaaaagcg ttcattcgtg agacactcct tttaaaattt tcttttatca
    10561 taccataaag cctactcttt tattcttgtt cttccgtaaa tttagacaaa aattaggtga
    10621 aaaataaaga atcaaacaac tagaatttat tccaaaagat aaagaaatat tcagcaaaaa
    10681 aaattttaca aagggttgcc tttttcaata aatttcgtat actaatacga gtggtagtaa
    10741 acaggaagtt tactattaga aaacttcgat tcggaggaaa ttatggaaat tggtgagaag
    10801 ttaagaaatt tgcgcatcca aaagaatttg acacaagaag aactaggcga acgcacagat
    10861 ttgtcaaaag ggtatatttc ccagttggaa cgagatttaa gttcaccatc aatggaaaca
    10921 ttcttttcta ttcttgaagt cttaggtgtg actccagaag aattttttca tcaagaagca
    10981 gcgaatctgc aagtggttta ttcaaaagaa gatcatacgg tttatttcga tgaagaaaac
    11041 gggtatgaat tggaatggct cgtggctgat tcgaatgaaa aagaaatgga gcctgtcttg
    11101 ctgacttttg aaggtgcagg agaatataag acgtttgaac cttcattatc tgaaacgttt
    11161 atttttgtat tagaagggga attagagtta agcttaggag agacggtcta tgtggcaaaa
    11221 gcaggacagt cgatctatta tcaagcgaca aaacaacatc aattaaggaa tcatctcaaa
    11281 aaacgaacaa aagtattgat tgttgcaacg caatcatatt tgtaatatag ggggtaaaaa
    11341 cgtggggaaa ccaatcattt catttgatca agtagtgaaa cgctatgatg atgaaacagt
    11401 attaaaaaaa gtaagttttg agatcgaaca aggaaaattt tatacactgc ttggtccttc
    11461 cggctgtggt aaaacaacga tcctacgtat tatagctgga ttcaccgaag ctacagaagg
    11521 ggacatctat tttgaaggga aacggttgaa tgatttgcca gctaataagc gacaagtaaa
    11581 tacagtcttc caagattatg cactttttcc tcatatgaat gtttttgata acgttgcttt
    11641 tggattgaag atcaagaaaa tgtccaaatc agatatcgaa aagaaggtaa aagatgcttt
    11701 gcgtatggtc cagcttcctg gatacgaaaa acgggagatt agtgaaatgt ctggtggtca
    11761 aagacagcgt gtagcgattg cacgagcaat tgtaaatgaa ccgaaagtat tgctattaga
    11821 tgaaccgtta tcggcgcttg atttgaaact acgtacagat atgcagtatg aactgcggga
    11881 tctgcaacaa cgattaggaa tcacgtttat ttttgttact catgaccaag aagaagcgtt
    11941 ggcaatgagt gatgagattt ttgtcatgaa taaagggaaa atcgtccaaa gtggtacacc
    12001 cgtagatatt tatgatgagc caatcaatca tttcgttgct gattttgttg gggagagcaa
    12061 tatcgtggac ggtgtaatga tcgaagataa tctagttgag tttgttggta agcaatttga
    12121 atgtgtcgat ggcgggatgc gtctaaatga accagttgaa gttgttttac gcccagagga
    12181 tttgacgatc acgacaccag ataaaggaaa actagtcgta acggtcgata ctcagctgtt
    12241 tcgtggtgtc cattatgaaa tcatctgtta tgatgaacag caaaatgaat ggatggtcca
    12301 ttcaacgaaa aaagctaaag aaggaagcaa agtaggattg gcctttgagc cagaagatat
    12361 ccatgtcatg cgtttcaacg agtctgaaga agaattcgat gcacgtcttg acagctacga
    12421 agaatagggg ggacaagaat gaaaacaatg cgcaggatct attccgtccc ttatattttg
    12481 tggctcggtt tattcgtgat cgcgcctgta ttaatgatca tttatcaatc tttttttgat
    12541 atgaatgggc aattcacgct gaataattat ctatcttatt ttacgtcggg gacatcgctt
    12601 tctatgacga tcaattctgt atggtatgca tttttgatca cgtttttcac gctgatcatc
    12661 agttacccga ctgcttattt tttaacaaaa ctgaagcaca agcaattatg gctgatgctg
    12721 attattttgc ctacatgggt gaatctgctg cttaaagctt acgcatttat tgggattttc
    12781 agtgtgcacg gcagcgtgaa tcatttcttt gattttatcg ggatcgggcc gaaacagata
    12841 ttattcacag acttcagttt tctgttcgtg gcaacgtata tcgagattcc atttatgatt
    12901 atgccaattt tcaatgcatt ggaagagatg aatccttctt tgatcagtgc tagtagagat
    12961 ttaggcgcga atagctttga gaccttccga cgagtgattt ttccgttatc attaaatggt
    13021 gtgaaaagtg gtgtccaagc agtatttatt ccttctcttt ctttattcat gctgactcgt
    13081 ctgattggcg gaaatcgggt gatcacttta ggaacggcta tcgaacagca tttcttggtt
    13141 acacaaaact gggggatggg ttctacaatc ggtgtgattt taatcattgc tatgttcatc
    13201 gtgatgctcc tgacaggcga gaagaaaaaa ggaggtcgct cataaatgaa aaaatttaaa
    13261 tggtcgtatc tttatttagg gatcgtcttt ttattcttat atgcaccgat cttctatctg
    13321 attttttatt ccttcaatga aggaggaacg atgaacagct ttacaggatt tacttgggaa
    13381 aattatgaag ctgtttttga agatacacgt ttgatcatta tcgtattgaa tacgttcatg
    13441 ctggctttct tatctgcatt gatcgccacg atcatcggga catttggggc tatgggaatc
    13501 tattacacga aaaaacgacg tgcacggaca gcgttgttga gcatgaataa catcttatta
    13561 gtctctcctg atgttattat cggtgctagt ttcttgattt tctttacact actaggattc
    13621 ggattaggtt ttcagagtgt attgctttcc catattgctt tcagcattcc aatcgtcgtc
    13681 ttgatggtct tgccaaaatt gcaggaaatg aacgattcaa tggtcgatgc ggctagagac
    13741 cttggtgcta ataatttcca agtagtcaaa aatatcattt tgccttatct ttctccagga
    13801 atcatagctg gatattttat ggcatttacc tattctcttg atgattttgc ggttacattt
    13861 ttcgtcacag gaaatggctt tacgacgctc tcagtagaga tctattctcg tgcaagacaa
    13921 ggaatcagtt tagaaatcaa tgcgctaagt gcgttagttt tcttgttctc catgatattg
    13981 gtcattggat actactttat tagtaaagaa aaccgtccaa aacggctgaa gaaaaacaga
    14041 agagtacgcg agggggtggc acggctgcga tgaaaaaatt acaatctctg tttttaggta
    14101 ttctcatcat tattgcggtg ctgctgcttg gtgttcgtca acttgaaaaa acaagtggca
    14161 tgagtggagc aaaggtactt accatctata actggggaga ttatatcgat cctagtttga
    14221 tcacgaaatt tgaaaaagaa tatggataca aagtgaatta tgaaacattt gattctaatg
    14281 aagcgatgtt cacaaaaatc cagcaaggtg ggacaaacta cgacatcgct ataccaagtg
    14341 aatatatgat ccaaaaaatg atcaaagaaa aactagtact tccattagat cattcgaaga
    14401 tcaaaggatt aaataatatt gatccacgat ttttagactt ggattttgat ccagataatc
    14461 agtattcgat tccatatttt tggggaactt tagggatcgt atataacgat aaattctttg
    14521 atgcaaatca aattaagcat tgggatgatt tgtggaagcc ggaattaaaa gacagtttga
    14581 tgctgattga cggtgcacga gaagttatgg ggctttcttt gaatagttta ggctattctt
    14641 tgaatagtaa gaatatggca cagttgacag aagcagcaaa taaattgaat catttaacac
    14701 caaatgtcaa agcaatcgtg gcagatgaga tcaaaatgta tatgatcaat gaagaagcct
    14761 ccgtggcagt gacattttct ggcgaagctg ccgatatgat gtgggaaaat gaacatttac
    14821 attatgtgat cccaccagaa ggatccaatc tttggtttga taatatcgtt atgccaaaaa
    14881 ccgtgaaaaa taaagaaggt gcctatgatt tcatcaactt tatgctagaa ccggaaaatg
    14941 cggcacaaaa tgcagagtat attggttatt ctacaccaaa tgaagaagca aaagcgttat
    15001 tgccaaaaga catttcgtcg gacgaacaat tctatccatc tgatgacacg atctcccatc
    15061 ttgaagtcta caaagatcta ggatcgaaat acctaggcat ctataatgac ctattcttag
    15121 aattcaagat gtataggaga taatggcaaa actcagtaag aatggggact tatcaacgtt
    15181 ctaaagagct aaaaatcgtc ctttttctat ctgagttttc caatcttcta ccaaaagcgg
    15241 atgaaacgcc gctttttttg tgttatttta ttttttctac cacgtttttc taccagagaa
    15301 aagtggacaa aaccattgta aaatagggcc cgagacgggc tagtaaatct aagattgaaa
    15361 aagttacaga aggataaagt aattggaaac tatgcaaata tacttaaccc ttctgtaatc
    15421 tattaataaa actaatatat gaatccagtt caatatttgt agctatgaaa aaacataaaa
    15481 atcatattag ataaaaacta aattggtaga taaattattt tgtcagatat ctgaggcaaa
    15541 tattcgacta tttgtgtttc aaagtaaaaa ttggaataca aatatagaat agacatattg
    15601 cagccgatag tttattatgc ttttagacac attaaactgg aggaaaaaat gaaaaagagc
    15661 gctaaagatc gagtttttga tgagctgata cgactaacag ggaatatgtc ttctgtcact
    15721 gccgagaaaa tttcgaagaa tctaggtatc tctcgtcaga atgccagcca ttatttgacg
    15781 agattagtgg aagataaaaa ggtcgagaaa ttaccgggaa agcctgtttt gtggaagcct
    15841 ttagatgaat atgcggttgt agataatacg gagcaaataa acgaagcctt tcattctgta
    15901 gttggtcatg acggaagtct gagagaggtc attcaaaaat gtatagccgc tgtcaaatat
    15961 ccgcctaacg gactcagtgt attaataaat ggagccacgg gtgttggaaa aagtttttta
    16021 gccacaaaaa tttttgaata cgctatccat gagcaaatta ttgaaaaaga tgcaccattt
    16081 gctatcttaa actgtgccga ttatgcggac aatcccgaac tgctttcggc aacactgttt
    16141 ggatacaaga agggggcctt tacgggggct gaaaaggata ccgatggttt attggcaacg
    16201 gccaacaacg gctatctttt tctcgatgag gtccatcgcc tatcgaaaga aaatcaagaa
    16261 aagctctttc tatttattga tactggaaac tatcgtccag tcggtgaaaa tgtaaattgg
    16321 cattctgcta aggttcgttt tattttcgcc acgacggaaa aaggtgaaaa ttatttatta
    16381 gacacctttg atcggcgtat tcaaattagt gttatgttgc caacttttga agatcgcccc
    16441 attcgagaac gattagagct gattcaatta ttttttcaaa atgaagcgga tgttttacaa
    16501 aaagatatta ttgtttcaag agaagccttg tctttaatgt tatctcatcc ctttagtggg
    16561 aatattggta agctgaagaa tatcattaaa atcagttgtg cggatgtgta tagtcgaaca
    16621 caagaagaac cattaacgat tgggaaaaat gagatcttga ttcagttaaa tatgctggaa
    16681 tccgaagtag aaagcttgcc gattgctagt ttattgataa agaaaaatca aaagataaaa
    16741 acggatcgaa tcaaaccgtt tgattatgat tcgattttgg aaaaaattac tcagctgata
    16801 aatgaaggta atttcaatga aaatttttct tcgataaaga atgaagtaca gcatttggtg
    16861 catcacattg ataaggattc ttttttatca atgagtagaa aattgatgat tcagatgttt
    16921 agaaaggtct gggtatccgt tttaggaaaa acgtacggat tgacgacgag tggacctgtt
    16981 gcagaaatta ttagcagatc ctatgtcatt tccccaagct ataagacaga cttaaccggg
    17041 ataaatcgta cattagctgc aaatctacct cggacgactt acttatgtaa tcagttcttg
    17101 gagcatttac caccgctctc agaaaaggac gagaactttc taagactgct tattgtcgtg
    17161 actttatctg attatgtaga tgaatccatc gagctgaagg gcctgttgct tgctcacgga
    17221 gatagtacgg ctagcagcat tcaagcagta gtgaatcaat tatgcggcaa ttatgttttt
    17281 gaagcgttgg atatgccagt agagagtaat gtgtcagaga ttgtcgaatc gacaaggaat
    17341 ttcgtgaaac ggcaagtgaa tacagatagt ctagtgctga tcgtagatat gggctcttta
    17401 aatcaaattt acacacagat caagaacgat ttaaaagggg agttacttct tctgaataat
    17461 ttgacgactt ccattgcact tgatattggc ttgaagatga cgatgaatgc gccatttaaa
    17521 cagattgcgg aggatgcagc agacaagtat tcaatcaatg ttcaatactt tgaaggcttt
    17581 tctcaaagta gcaacattat catttcctgt atgtcgggtt tggggatttc ggacaaattg
    17641 aaagatgttt ttcgtcatca tttatcggat gaatccatcg aagttttcac gaaggactac
    17701 agagaactac gtgaattaat cgaccaaaat gatgaaactt attttgacaa gacaaaattg
    17761 gtcatcacga cttcggatct tccacgttcc ttttcaattc ctaatattaa tgtttatgac
    17821 atcttagacg gtgatggagc agccaatttg gagcatattt tgagtcctga aatgaagccg
    17881 gcagaattta accgcttgat tcaggagcta gtaaaattct tttcgttgga aggaattgcg
    17941 gaccgtttga actttctgaa cccaacgatc gtgatgagtg aagtggaaac agtcatttct
    18001 aaatatgaaa attactatca tttcacttta aatggccgtg taaaactaaa tctctatatg
    18061 catacggcat tgatgatgga acgtttattc ttggctcgga cagaaaaatc tgaagaaccg
    18121 aattatgatt tgaaaccaga tgaagatgaa ttttattatg tttctagaag tatttttcaa
    18181 tcaatggaga tgaaatataa ttttaaagtc aatgcttatg agctttcgct gctctatgaa
    18241 ttattaggac cgtatattca aaaataaagt gtctaaaaaa gtgtctaatc tgtttttttt
    18301 aggttagaca ctttttttaa tgtgaattaa cgtgattaaa ccaatgtttt taaaagttgg
    18361 cacaggaatt gcttatataa aagtgaacag aaagaaagga attaaccata tgaagaaaat
    18421 tctaatagcg actcacggtc acttagcttc aggctttctt agcagtattc aattactgac
    18481 ggggaaggtt caagagatta cggtgattaa tgcttatgtg gatgagtgtg attttgaaca
    18541 ggagttgaat gtatttgtaa accaggtgaa cgagcaggat cagatttttg tttttaccga
    18601 tttatttggc ggaagtgtca atcagaagat taccaaaacc tttttggaga aagagattgc
    18661 tgtaactatt ttgtctggct ttaatctgcc aattctgctc gaaatagtat tggcaaccaa
    18721 ggaattaaca aaggaggata ttcaggaact ggtaaataaa tgccaacagg aattaaagat
    18781 gaccattctt gatgaggtac atgtagtaaa tgaagaggaa gatttttttt aggaggaaga
    18841 aaaatgatta aagcagtgcg aattgatgaa cgattaattc atggacaagt agcgatgact
    18901 tggacaaaga gtctaaattt aaccgggtta gtagtagcaa gcgatgaggc ggctaccaac
    18961 gatattcaaa agatgacatt gaagatggct gcaccatcga atgtcaaagt cattattaaa
    19021 acagttgatg acgcgattca tttgttaaaa gatccgcggg cgaaggacat gcgcttgatg
    19081 gttttagtcc ctaatgtcaa agatgcggta aaagttgcga aagctttccc aacggaaatc
    19141 gaattaatga acgtggggaa tgctgggaaa atgacaccgc catcggaaga taagaaaacc
    19201 ttatcaaagg aagtgatgtt aacgacgtca gaaattcaag acttaaaaga attagttgaa
    19261 ctttatccag ataccgtttt tcagcctaca ccggcgatgg aaaaacgttc agctatctct
    19321 attttgagtg atttttagaa aaaggaagga gaaaaattat gtatgatgca tttgtagtcg
    19381 ctttggccgt ttttattgga attgctggac atgaaatgtt cgggatgtcc atgctgagtc
    19441 gtccgattgt tgttgctccg ttagcaggag cgttgatggg ggatttgcag acggggttga
    19501 ttgtcggggc gtcactagaa gccatcttta tgggtgtcgt caatatcgga atctctagta
    19561 cggcggagcc ttctttggct gctgggctgg ctacagcgtt cgcaatcaaa atgggaggaa
    19621 acgtggatgc gattattccc attgtctttc cactagcagt attaggatta cagttaatga
    19681 atatgatttt ttcttttgta tgtggaccga tggctgctaa atttttcaaa tttgcaaaat
    19741 ctggtgatca aaaaggaatc atcaatctgc acttctttat gtggtttgtc cactatgcgc
    19801 tttatgctct tattccattc tttgccgttt tatttggagc agatgttgtc aaaagcgtct
    19861 tggataatat tccagaggtc atcatgaacg gattaacagt cgctggaaat ctgctgccgg
    19921 cagttgggat ggcgatgctg ttacggatgc tttggagtaa aaatattgct gtgtggtttt
    19981 tctttggggc cgttgttatg gcgtatctcg agttaccatt gattgcaatt gccgcagttg
    20041 gaacgattat tgcaattgtt atagcccaaa gagatctaca actaaaaaat atatctgttc
    20101 atgcagttca aagcgatgct gctgtaaatg caacgcttga ccaagaagag gaggatttct
    20161 ttcaatgaaa ttagctgatg gaatttctaa agatgagaaa aaattggttc ggaagatgtt
    20221 ttggcgctcg atgactatgt atgcttcggt gaatccggtg acgatgggcg gcggtggttt
    20281 cgcctattct atgatgccct tcattaatca tttctacaaa agtgaagaag atcgagctgc
    20341 ggcgctggaa cgtcatacac agtatttttc gacgaccatt ccttgcgcgt cctttgtcat
    20401 ggggattgcg gcatcgatgg agaaggaaaa ttctgaaaaa gctggttttg acgtggaaag
    20461 tatcaacgcg atcaaaacga gtttgatggg accattggct ggaattggcg attcattgtt
    20521 ctggggagtt tggcgtgtcg ttaccgcggg actagcgatc aacttagcca atgccggaaa
    20581 tattttggca ccaattatct ttttagttat gttcaatata cctaattact tgatgcgata
    20641 ctatggcggc gttttaggct attcattagg ctctaaatat attgaaaagc tgtatgcgaa
    20701 cgggttaatt gaaattctga caaaagctgc tagtatcttg ggtctgatca tggtaggtgc
    20761 aatgacgatc agtatggtca aattcaatac cgttttgagc ttttcaatga agggcgaaga
    20821 actaatgaat gtccaagaaa tcctcgatag tatttttaaa ggaattgtac cgttattggt
    20881 aacattgagc tgtttcaaat tactagataa aaaagtcggc gtcatcaaaa ttatttttgg
    20941 aatggtcgtc ttgggaattt tattgtcatt acttggaatc gcatagtagg aagctgcagc
    21001 tactaggctg cagttttttt agcaaggagg gaaaagatga ctattaatga agtaatttat
    21061 tcacccaaac acccattttt taatgctgag ttaaacgacg gaaatgtgac tacttcaaac
    21121 gaatacagct actatcggaa tattcctgat gtaaagggat tgaccttcga tgtaagtaat
    21181 caaaagacat tagcaaacct tgatgtttgc gggacgattc aaacgttgac cttttatcga
    21241 gaaaatttac taactgagga aaaaccaggt gtttgggtca ataaacagct ctctcaaacc
    21301 aattcgttat ccttaaaggt tgaagtaaac gggattgtct accatttgtc agaatcggat
    21361 catcgtattg aagtcgatgt gattcaagac tgtttgccta gatatattca tcattattcg
    21421 acgtttaagg tcattgtagt atcctttgct cccatactgg ctaataagcg tttaagtatg
    21481 cttgttcaac aggtttacat tgtgaatgaa acacacgaaa gccttcaagt ttcagtgaaa
    21541 gatgtgcctt cgtatcaaga gaagtattcg gatcagcaaa atgtgttgat ttctcaaaaa
    21601 ggaaataagg acacgatcgc acaacaggaa gtggcaagct tcgccattgc ttttgtggac
    21661 ccaaatgctt ttgaagaagt aatgactttt caggaatctg atatcgaaaa atggctgaag
    21721 gaaacattga actattttgc tcagctattt gggcagctgt caatgccaaa cgatagagtt
    21781 gtccatctct atcgtcgtgc actttatcaa agcttttcta gtttcggtat gaatcgacag
    21841 ggacaggtcg tcggcagcaa ttggggcagc tatcctgcta ctaatcgaat ttggaataag
    21901 gatatgtatt acgcatcatt gccattcacc atgtttgaac cggagttatg tcaaaaaacg
    21961 gttctatggt ttgatcaata tggcgttaag tttcctggtt caaaatttac aggaggtatc
    22021 aatcattctc tgagtaattc gttatccagt gcgttactgg ctagtcttta ttttgaacat
    22081 actggggata caaaattctt tgagcggtgt ccgcaggttc tactgaatgc ttcaagaatt
    22141 attgaggaca tattagctca acgtcggtca ggggaaccaa tgctttttcc atctgtttgg
    22201 ttatcagacg cctttgcact tggaaagtat cataccggca gcaatctttg tctttggaag
    22261 gcctgtattg gacttgcaga aatttttgaa gccctagaac aactgagtct tagcaagcga
    22321 tacaaaaaca ttgcctgtaa actaaaggaa tcaatttgta aacaaatgac tatcgatggt
    22381 ccttttggtg agcaattttt ggagggaatc ggtgatgagg agaaaacaac ctattcagtg
    22441 aagcactatc aaaaaccgat tcttgagcaa gggttaatct ttttaagtga tgtgattgtg
    22501 gatggaaaaa ttaatttatt gatgcatgat ggagaagaat cagacactac gttgatgcct
    22561 ttttatcaat ttttagataa taaagatcat ttatatcaga acaccatggc cttttctgct
    22621 agttctttca atccaaccta tagcgaggaa atcaaaggca tcacttgggg attagaatcc
    22681 ggcgccactt ttcctggctt tatcactgtt ttaatgagcg agctgcacaa ccctcaagct
    22741 ttcgtaaccc gattagaaga actaatttgt ttggcagatt tagacggttc gtggtggtgg
    22801 tggccgtatc gcttagaggc tcaacaagga gaagtcgtgc gagactttgg ctgcggcaaa
    22861 tgcggctggg catcgggtct ttttgtcagc ctaatgatca gtcaatattt cggattgaaa
    22921 ttcaaaaaag gagtattgca aattgatcct gttgataatt taacgttttc ttggaagaat
    22981 ttgaagttcg gtcaagcctt catttcgatt gaatgtacgc aatccgagtt gagtatttca
    23041 aatttgtcag aaaagccatt gaaaataagt gatacgaaaa aaattcttca cgtagaaaag
    23101 ggaaaaacga ttcacattca aaggagaaaa agatgagtat actaaaaaat attacccaat
    23161 ttaaaaaaat ccagagtcga gcaatcactc ccgaaaatcc aacaggcgag aaaggtaaag
    23221 ctgcgatgtt gccaagcgag ctaggaccaa gccgcaaagg tcgtggcgca attccgctga
    23281 cttctggtga agaaactgtg attgcggata ttactggaac gggaataatt cgtcatatgt
    23341 ggatgacgat tcgtgatcga acagaatttg gcagttttgt tttacgtgat ttaatcttaa
    23401 gaatttattg ggatgggtcg gatagccctg ctgttgaaag tcctttaggg gatttctttt
    23461 gtaatgggtt tggagagcgt tgcgaggtga actcgttgcc gatagtggtt aatcctacag
    23521 ggggctttaa ttcctatttc gaaatgcctt tccaaacatc cgctaaaatt acgatcgaga
    23581 atttacatcc gaaggatatg tcctcatttt tttatacgat aaattacgct ttggtggacg
    23641 aattggaaga aaatagccta tattttcacg caaattggca tcgggaacgc attaccaaat
    23701 tacaggagga ttataagtta attgatcgat taaagggtca aggctattac gtgggtactt
    23761 accttgcgct tacctgccta gaacgttatt ggtggggcga aggcgagttt aaattctaca
    23821 ttgatgggga tagtgagtat ccgaccgtaa cctcaacagg ttcagaagat tattttggcg
    23881 gggcgtgggc ctttcatgag tacgatgagt tcggacgtcc acatgcgaaa aactattcaa
    23941 cagccttttt agggtatcct taccaatcaa atagagatgc cacaagagat cgttttgaaa
    24001 cgggaaaatt gaatgccgtt catgcctttg gtgacgacgc aatgccacga catggtcttt
    24061 atcgttggca tcttcaagac cccatctgtt ttaccgaaga tttatcggtc actttacaac
    24121 aaatcggcaa cgatgatgtt cgcttatttg agcggcaaga cgatgttgct agtgtcgctt
    24181 actggtattc aacagaagct tcaggaaata ctaaacgtca tttcactgat cgagaacgct
    24241 tgccgcgtta atgaagtgtt tttcacatat tcgaagggag gaacctatga tgatatatgg
    24301 ggcaattgaa gctggaggga ctaaatttgt ctgtgcagtc ggtactgagg aattaacgat
    24361 tttggaaaga gtcagttttc ccacaacaac tccagttgaa acaatgaagc aggtcattga
    24421 tttttttaaa ccttttaagg aacaattggc aggcatcggg gtcggctgct ttggtccaat
    24481 tgatattcaa tgcaaatcaa cgagtttcgg tcatatcaca tcaacaccaa aacttgcttg
    24541 gcaaaatttc aattttgtag gaacgctaaa gcaatatttc aatattccga ttgaatggac
    24601 aacagatgtc aatgcggctt gctatggtga atatgttgct ggttctggca aagggttagc
    24661 aggtgttgca tacttcactg tgggaacagg tatcggaggt ggtgccttgt tcaatgggac
    24721 tttcgtggaa gggttcagtc atccggaaat ggggcatatg ttgatcaagc gacatccaaa
    24781 ggacaatttt tcaggaaact gtcccttcca ccaagattgt ttagagggga tggcagcagg
    24841 accagcgatt gaaaaacggt tgggtgtaaa aggccagaat ttattggctg acgattcgtt
    24901 ttggcagatt gaagcctttt accttgccca atgtgcatac aatacaacac tgatgttttc
    24961 gccagataga attatttttg gcggtggtgt aatgaaacaa gaacacatga agaaaaaagt
    25021 tcaggataaa tttgttgagc ttataaatgg atatgttgaa ataccaccaa ttgatagcta
    25081 cattattacg cccgaattag gtgataatgc cggaataatc ggcggattag cattggccag
    25141 aaaagcggta agaaataagc aaccataatt aatgaatgtt tcattgatac tcatgtcaaa
    25201 agtatttaac cgagtaatca aatgatcaaa tgaagttgga taaagcgaaa atgaattcaa
    25261 ataaaaaggc tctaagtcaa atcgtgttgt tttcattaaa tcactgaaag gaaatgcttc
    25321 tgcgtttcct tttttctttt tgttgtgcgc tgtatacctt gaggatattc actggtggcg
    25381 tacacttaaa tagtaatata ttgtgttact ttatgtctgt ggtaatagtt atagttgctt
    25441 accaatgtat gttcattttt cgaaatgttg acttcataat ccttggctta atgtttgaat
    25501 ttttttggtg atagattttt tcagccgtat aaaataaatc gtaattctat tatagagagt
    25561 tgtgattata aaaagatagc aagaaaaaaa aagaaagttt ctatacattt taaatatttt
    25621 attcctattt ctattgctaa aaaagtagaa attctatatt tacctttaac tctcgcttct
    25681 cttgcaaatg tagttagcat tttaatcgat aataaactca ggtagttata ttaccttgaa
    25741 atgattgata attttgtcac ttctattttt tttgcaataa attttttatt ttagtgaaaa
    25801 ataagaaact atttctttga aggcgataag ttagaaaaat attattgtag tcttttcttt
    25861 attaggaatc atcactgttg tatcttatta ccttttctgt ttttcttatt agagaaaaat
    25921 aatttgagag gtagatagtt tttattggag gaagtaatta tgtatatttt tgaaacaaaa
    25981 atttttcatt gcaataattg caacaaagaa atttcatcgg gggaagaggt ttggacaaaa
    26041 atggaaattt ccgccaaaag taactgctcc tcaactgaaa tcaattaaag aactagaatt
    26101 tgagaatgca ctggtaattt gtatgacatg tactgatgaa gtatttcgaa aacattttta
    26161 aaggttgaga tgtatataga tttaaccaat catttatctc tctcggaaaa ggaatattaa
    26221 ctaaccagcg ggttatcaaa aataggtagg aaaactgtac ttaatggctt atttcgcgct
    26281 gattatgaga atatcagtct gtttgattaa tttttagggt tcaaaataca gagacaaact
    26341 ccaggtcggg tttcgccaca agcgaattac ccgatctgga gttttgtcta ttgcaggaag
    26401 gcgtagccgg actgcaggaa taaagccccc ggtt
//
LOCUS       DARBFD010000029        25627 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000029 DARBFD010000000
VERSION     DARBFD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.029"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            46..468
                     /locus_tag="SCK08_001190"
     CDS             46..468
                     /locus_tag="SCK08_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201095.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(634..1827)
                     /locus_tag="SCK08_001191"
     CDS             complement(634..1827)
                     /locus_tag="SCK08_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1201096.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2051..2428
                     /locus_tag="SCK08_001192"
     CDS             2051..2428
                     /locus_tag="SCK08_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1201097.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2416..2754
                     /locus_tag="SCK08_001193"
                     /pseudo
     CDS             2416..2754
                     /locus_tag="SCK08_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2888..3331
                     /locus_tag="SCK08_001194"
     CDS             2888..3331
                     /locus_tag="SCK08_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEG1201098.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3592..4407
                     /locus_tag="SCK08_001195"
     CDS             3592..4407
                     /locus_tag="SCK08_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1201099.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4417..5079
                     /locus_tag="SCK08_001196"
     CDS             4417..5079
                     /locus_tag="SCK08_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1201100.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            5287..6951
                     /gene="alsS"
                     /locus_tag="SCK08_001197"
     CDS             5287..6951
                     /gene="alsS"
                     /locus_tag="SCK08_001197"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEG1201101.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            6964..7674
                     /gene="budA"
                     /locus_tag="SCK08_001198"
     CDS             6964..7674
                     /gene="budA"
                     /locus_tag="SCK08_001198"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEG1201102.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            7804..8301
                     /locus_tag="SCK08_001199"
     CDS             7804..8301
                     /locus_tag="SCK08_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEG1201103.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            8486..8746
                     /locus_tag="SCK08_001200"
     CDS             8486..8746
                     /locus_tag="SCK08_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201104.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(9108..9347)
                     /locus_tag="SCK08_001201"
     CDS             complement(9108..9347)
                     /locus_tag="SCK08_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201105.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            9633..9947
                     /locus_tag="SCK08_001202"
     CDS             9633..9947
                     /locus_tag="SCK08_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201106.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(10381..10747)
                     /gene="ssrA"
                     /locus_tag="SCK08_001203"
     tmRNA           complement(10381..10747)
                     /gene="ssrA"
                     /locus_tag="SCK08_001203"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(10918..11691)
                     /locus_tag="SCK08_001204"
     CDS             complement(10918..11691)
                     /locus_tag="SCK08_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1201107.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            11835..12185
                     /locus_tag="SCK08_001205"
     CDS             11835..12185
                     /locus_tag="SCK08_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEG1201108.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            12166..12882
                     /locus_tag="SCK08_001206"
     CDS             12166..12882
                     /locus_tag="SCK08_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1201109.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            12882..14330
                     /locus_tag="SCK08_001207"
     CDS             12882..14330
                     /locus_tag="SCK08_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1201110.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(14400..14909)
                     /locus_tag="SCK08_001208"
     CDS             complement(14400..14909)
                     /locus_tag="SCK08_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201111.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(14899..15624)
                     /locus_tag="SCK08_001209"
     CDS             complement(14899..15624)
                     /locus_tag="SCK08_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201112.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(15847..17967)
                     /locus_tag="SCK08_001210"
     CDS             complement(15847..17967)
                     /locus_tag="SCK08_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1201113.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            18197..19375
                     /locus_tag="SCK08_001211"
     CDS             18197..19375
                     /locus_tag="SCK08_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEG1201114.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            19391..20149
                     /gene="aroD"
                     /locus_tag="SCK08_001212"
     CDS             19391..20149
                     /gene="aroD"
                     /locus_tag="SCK08_001212"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEG1201115.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            20172..20657
                     /gene="ybaK"
                     /locus_tag="SCK08_001213"
     CDS             20172..20657
                     /gene="ybaK"
                     /locus_tag="SCK08_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEG1201116.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(20728..21981)
                     /locus_tag="SCK08_001214"
     CDS             complement(20728..21981)
                     /locus_tag="SCK08_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1201117.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            22099..23448
                     /gene="gdhA"
                     /locus_tag="SCK08_001215"
     CDS             22099..23448
                     /gene="gdhA"
                     /locus_tag="SCK08_001215"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEG1201118.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            23560..24906
                     /locus_tag="SCK08_001216"
     CDS             23560..24906
                     /locus_tag="SCK08_001216"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEG1201119.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(25214..25600)
                     /locus_tag="SCK08_001217"
     CDS             complement(25214..25600)
                     /locus_tag="SCK08_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1201120.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         8478 a         4418 c         5119 g         7612 t
ORIGIN      
        1 ttttcttgta tgtagtatag taaagtagat acaggaggag aaaatatgca gactttttta
       61 acaaaagttt ctcaaaagaa aattaaaatg attcatttac tgcttgaaac agaacgttgg
      121 tgttcaatag aagaactaca aaacgaattg aaggtatcaa gtaagtctat cttgaattat
      181 ctaacagatt tagaagaatt gttccaacaa tatcctgata aggtcatttt gaagaatgag
      241 aataatcggc gcttttctat ggaaaagcag gagaacttcc caatctacat tatttatctt
      301 catttctaca gaaaatcata taattatcat ttgattgaat tcatgtatca acatcctgag
      361 aaaaatttgg aagattatgc agacgctcaa tatacgagtg tatccacagt ttttcgttat
      421 gctaagttat tagtaaagta ctttgaaatg acggacgtca aagaatagtc gccgccagat
      481 agaaggaaat tagcagttga aaaagctgcc ctgcatcaag taatacgacc agccaaacgg
      541 aaacagtgtc tcatgttttt gatttggctg gtcttattac cgcagatgca agctttttcc
      601 acacctcttt cttgtcattt acagactcaa ctatcaatgc tgcaagcttt cccttaatgc
      661 tgtaacacca gtaagagttg tcaagaccag cagactgact aggtaaatac cgctcaaaaa
      721 caagattacg gcagttaaac tataatgatc catcaagaaa ccgatgacga tagaagaaaa
      781 accaccaaca gctcgtccaa ggttcataac gaaattattc gccgtcgcgc ggatttctgt
      841 tggatacaaa ctactaatca ctgcgccata tccaccatac atgccattga taaagtagcc
      901 gactatcaca gcggctgcta ccaaactcca ctggctatgg gctaacacaa gtagatagac
      961 agataaggat gaacaaatta aaaacaaaga aaaggctagt ttaggcccca atttatccat
     1021 gattatgcca aaagtcatca tccccaaaga catgcctaaa atagtactga ccatccacaa
     1081 tgaagaccca gaaaccgaaa gccctaatcg attttgcata attgaaggta gccaattcat
     1141 taacccaaaa tagccggcaa tttggacagt taccatgata ctaaggccac atgtttgcca
     1201 agcaagatgt tttgattcaa acagtctgct aagtttggtt ttcttttcgg cagattgtac
     1261 agatttgaat tcctttggtt cagtcaagcc gcgacgaatg aacaagacca tcaacaccgg
     1321 cagtacacct acgacataca acatcttcca accaaacgtt ggaatgatca cagcagctaa
     1381 aatcgcagcc aaagccgcac cgacctgcgc accaatacca gcaaccgaag aagctcgtcc
     1441 aatttgtttt tttgaaaaac tttcggaaat caaggacata cacgccccat attctcctcc
     1501 cgctcctaca cctgcgataa agcgaaagag ataaaccaag tagatgtttg aagcaaaata
     1561 catcaataaa gaagcaatcg aaaacagaag gaccgtttga gaaaatactt tcacacgtcc
     1621 atatttatca gctatgatgc caaaaaaaat acctccaatg agcatcccca aattagtgat
     1681 cgttgaaatg aaacccgctt gcgtaccact aatatgaaaa gaagtaatca ttgaagataa
     1741 tgaaaaagct aaaaacataa tatccatatt ctctaaagca attcccgccg atgtcgatac
     1801 taataccttt cgctgatacg ttgtcatttt tatacccctt ttcctaaaat ttccctaaat
     1861 tgttaaaagt atatccaaaa aagaataaaa aaacaagact tttttatttg aaataataca
     1921 caaacaaccg atatagataa tccagcttag ttttaataga aatgagtatt gacttggagt
     1981 acacttaaag ttgtagaata aataaaatac ataagtaaag tggcaagaaa ttaagagaaa
     2041 gagtgatcag atgaatatca aacaagtcag tgaagaaaag ggcatttcgg ccgatacatt
     2101 gcgctattat gaacgaatcg ggttgattcc accagttaat cggacaaaag gcggaatccg
     2161 tgattataca gaagaagacc taaaatgggt cgactttacg atctgtatgc gaaatgccgg
     2221 attatcgatc gagtctttga ttgaatatat tcgcctatct agcgaaggag aagcaactgt
     2281 atttgagcgg cgtaagcttt tgattgaaga aagtgaactt ttgacgaaaa aaattgctga
     2341 aatgcaagaa tgtttggaac gtttacaagg aaaaatcaag aaatatgatc gtgtgttatc
     2401 tagaaataaa atggaatgta tcaagtaaaa aaatcaagga gcatcgctat ggagttacgc
     2461 ttactaaaat atttttggac aattgcagaa gaaggaacaa tctctcaagc cgcagaagtt
     2521 ctacatctta cacaaccaac cttaagccga caactgaagg agttagaaga gtaactagga
     2581 acagaactgt ttgtacgcga aaaccgcaaa atgatcttga cagaagcagg ttattttcta
     2641 aaaaagccga gcagaagaaa ttttagattt aacgaatcaa accactcaag aattcgagaa
     2701 cagaaaaaaa caattattca gcggccgatt tacgatcggc tgtgtggaag ctgatagttc
     2761 gaatacattg gcactattac tagaaaagtt gatccgtgat tatcccagcg ttcaatttca
     2821 tatccacagc ggaacaagtg atgatatcac ggagtgacta gaaaaaagcc tgttagattt
     2881 agcgattttg ctagatccag tagatacaga gaaatacgaa acgattttgt taccaagaaa
     2941 ggaaaaatgg ggattgctca ttttagaaaa ttctgtttta gcaaaaaaag acgtgataac
     3001 aaaagaagac tttgcgaatg tacctctttt gatctcaaag cgtccagaaa tacagcgaat
     3061 gtttgctgac tggttaggca taaaaaagga gcagttgctg atcacaggaa cgtacaattt
     3121 gattttcaat gtatttgcgc taattgagaa tcaggtaggt gctgctttag tcatagaagg
     3181 tgctgtaagt catcattcaa agcgagtgac attcgttccg cttgacccaa tgattgaaac
     3241 gaactgcatg ctagtctgga aaaagaatcg gattttatca cctgtagtca atgaattgat
     3301 tcaccgtttc aaagaagctt ttgaacagta ataagtatct ctaatcgaag aaatttcaat
     3361 acaaaagcga gacgtatgac agtcttgctt ttgtattttt tcatttttaa atcaaaaaaa
     3421 taaagagatt tacataactt gttaaataag cgcttacaaa aataagaaga acggaaatga
     3481 atcagctata ataaccgttg ttctggattg taatcattac aaagaaatga agattataac
     3541 tttggaggtg ttcgaaatga aagaaaaaaa gcaaaaattc gttcaaaaaa catgcccttt
     3601 attcaatggt tgggtatgat tctgctgatt gtattagctt tattgacgat cattcctttt
     3661 ttatggatgg tttctgcttc attcaaagca aataatgaag tgttcacgat tccgattcaa
     3721 tggatcccta aaacttggca tttagagaat tatctggtta tttggtctcg tattccttta
     3781 gtgacatttt ttaagaatac agctttttta agcgttgtga tcacgttgat tcagttgttt
     3841 acttctagtt ttgctgctta cgggttttct aaactaaatt ttaaagggcg cgatatttta
     3901 tttttagcat atatcggcac gattgctgtc ccttggcagt cttatatgat cccgcagttc
     3961 attatgatga gaaaagtggg tttaacagat acgttgtggt cgttggtgct attacaagcg
     4021 ttcaatgctt ttggtgtgtt cttgttgaag caatattaca gtagtatccc agattcttta
     4081 tgtgagagtg cgcggatcga tggcttatca gagtggggaa tctatcgcaa gatcatttta
     4141 cctcttacaa aaccggcgat tgccagtttg acgatcatca cattcgttaa tacatggaac
     4201 gattatatgg ggccattcat ttatttgtct tctacggaaa ataaaacaat ccagttaggg
     4261 ttgaaaatgt ttattgggct atttgatgca gagtacgcat taatcatggc tgcctcagtt
     4321 gtttctgttc ttcctgttgc ttttatattt ttagcgatgc aaaaatattt tgtagaaggg
     4381 atcgcttcat caggaatgaa ggaataggag gtgattatga aagagcagcg ttttgacaaa
     4441 aatgtttata tggaattctt ttattggttg tatcaatcat tggcaagtag cctttgtttt
     4501 tggtgtgtca atatcttgta tatagcagct gcgttccttt tgtcgataga tggaaggaat
     4561 gtcttttcct ttattttttc tttacttttt attggtccag gttgcatcgt gatgataggt
     4621 atgacagaag aattagcaaa agaaaatagt ttttcaccag tacgtttgtt cataaagatg
     4681 tttcgccaat tttggctaaa gggatttttt tattggctat ttgcttggat agttagtgtg
     4741 ataatgattt ttgattgttt cttttttata cgtttttctt atggaaaatg gttgattccg
     4801 ttgtttgttt tacttgcttg tttatcagtt tcctttagca ccaactgctg gtattttcag
     4861 gtgagaaatc cagcgtcgaa accaaatcaa gttctacgga tagcgttcta ctatacgttg
     4921 aaaaaatggt atgtgagttt actagatttt ttactattga caagtttgtt tctcttcttt
     4981 ttcgtaaagc cccaatggtg tattctacta ggtccttcta tcgtatttgg tttgatttac
     5041 ttcaacaatc gtaaattgat gagaactatg gatctgtaga ctttataaat tgaaactttc
     5101 aattaagaat gacagttttt tttatgtaaa aactgtcatt ctttttcatt ttttgaaaca
     5161 tatggctaaa caaatgaaat aaaacgtgct atacttttgt atgaaacagg tttagaaact
     5221 gtatcactta tttgtgatta tttctaacta ataattaaaa ataaagcata aaaaggacga
     5281 tgttcaatgg ctgaaaaaat gaaacaaggt tcgaaatctg tagtagaaag tttgattaat
     5341 catcatgttg attatgtatt cggtatacca ggtgcaaaaa tcgatggtgt atttaatgaa
     5401 ttagaggatc aaggtcctga attaatcgtg actcgtcacg aacaaaatgc tgcatttatg
     5461 gcacaagcaa tcggacgtat tacaggagag ccaggagtag ttatcgcaac aagtggtcct
     5521 ggtgctagta acttagctac aggtttggta acagcgacag cagaaggtga cccagtttta
     5581 gcaattgccg gacaagtaaa acgaagcgat ctattgaagt tgacccatca aagcatggat
     5641 aatgcagcac tgttccagcc aatcacaaaa tacagtgcag aaatccaaga tccagaaaca
     5701 atttcagaag tgattgccaa tgcttatcgt atggctaaga gttcaaaaaa aggcgcgagc
     5761 tttatcagta tcccgcaaga tgtagtggat gcacctgtcc aaggtaatgt catcaagcca
     5821 ttgagcgatc ctaaattagg ttctgcttct gcagatgata tccgttacct agctgaacgc
     5881 attagagaag caaaactacc ggtactatta gtaggtatgc gaggatcaag tgaaaaagaa
     5941 acgttagcca tcaggcaatt agttgaaaag acagcattgc ctgttgtaga gacattccaa
     6001 gcagctggag tgatctcacg tgaattagaa gcgcatttct ttggtcgtgt gggtttattt
     6061 agaaaccaac caggagatat gcttttgaaa cgaagtgatc tagttattgc aattggctat
     6121 gatccaatcg aatatgaagc aagaaactgg aatgcggaaa aagatgcgcg gatcattgtg
     6181 atcgatgaag cgccagctga aatcgatcct tttatgcagc ctgaaagaga attgatcggt
     6241 gatatctcag ccactcttga tttattgaca ggttcattag agcctcaaca agtgtcagaa
     6301 gatgcaaaag aatatttagc atctcttcaa gcaaaattaa cggagcggga cattgttcaa
     6361 tcaaaaggag aagcaggtat cttgcatcct ttagaagtta ttaacacgct tcagtcaaaa
     6421 gtaacagatg atatgactgt taccgtagat gtaggtagtc attatatttg gatggcacgc
     6481 cactttagaa gttatgaacc tcgtcatttg ttattcagta atggtatgca aacgcttggt
     6541 gttgcattgc catgggcgat ttctgctgca ttggtgcgtc caaatacaca aatcgtatca
     6601 gtttccggcg atgggggatt tttattctct gcccaggatt tagaaacggc ggtacgtaaa
     6661 aaattgaata tcgttcattt gatttggaat gatggacact acaatatggt agagttccaa
     6721 gaaaaaatga aatatcagcg ggcgtcaggt gtagactttg gtccagttga ttttgtgaaa
     6781 tatgcagaag cttttggcgc taaaggaatc cgagctacaa gtgtagagga attagaaaaa
     6841 gctttagaag aaggatttgc gacagaagga cccgtaatta ttgatattcc gattgattac
     6901 cgtgataatg agaaattggg agaaacaatc ttaccagatc aattctatta aggaagtgtc
     6961 gacatgactg aaaaaatctt ataccagcac gggacattag gagctttgat ggccggcttg
     7021 atggatggaa cagaaacgat tgctcatata ttagaaaaag gaactctggg attgggaaca
     7081 ttgcatgggc ttgatggtga agtcatcttt ttagatggcg tagcttatca aggaagatca
     7141 gatggctcag ttgcccaatt agatggaagt gaacttaccc cctatgcagc aattacagat
     7201 tttacgccag atactagttt ttctgtctca aaaactgcag atggagaaca attgaaaaaa
     7261 gacatattag taagagaagc cggggaaaac ctatttttgg cagtgaagat aactggatta
     7321 ttcaaaaata tgcacatccg tatcatgccg aagcaagaaa aaccttacag aagactagca
     7381 aaaatttcag agtctcaacc agaattccaa caaagcaata tccaaggaac attagtcggg
     7441 ttctttacgc ctgaattatt ccaaggagtt gctgcagcgg gatttcacct gcattttatc
     7501 gatgatacac atacatttgg cggacatgta atggattttg aagtggctga aggaaaagtt
     7561 gagattagcc gcatctcgtc ccttgtacag cattttccag ttgatgatcc gacttttgtt
     7621 cacgaaacga ttgattatgc tgatttagca gcagaaatcc aacaagcaga atagaaaaag
     7681 acagcgagat gcgttcaagg tgattaacct gacgtatctc gtttttttga ggaggaaacc
     7741 tagcgtttac aaaaaaactg agctataatc ttctccatag atcaaaaagg agcggttttt
     7801 atcatgtgga cagaaaaaca gaaaaaagat gcatacgaaa ttttattatt acagcaacaa
     7861 ggattattag aagcagaaga taactggtta gcaaatttag caaattcatc tgcattgttg
     7921 aatgagactt tgcctgagac tgtctttgca ggatattatt tattcgatgg taatgaattg
     7981 attttaggac cgttccaagg gcgagtttct tgcacacgta tcaagatggg aaaaggagtt
     8041 tgtggagaat cagctgaaaa acaagaaaca ttgatcgttg atgatgtcaa aacccatgca
     8101 aattacattt cctgcgattc agccgcacaa tcagaaatcg tagtgccaat ggtaaaagaa
     8161 ggtaatttaa tcggtgtttt agatatcgat tgcggagtaa cgaaagggta tgatgaaatc
     8221 gatcaagcat ttctagaaaa atatgtagat cttttattag caaattttaa tataaatgct
     8281 ttttcattta ttaataacta aaatcgtcta ttgaaacaaa aaagaagaga aatcaagaat
     8341 aaaaagagcc ggaaagacag tggtcatttc tgtttttcgg tttttttatt tttttataaa
     8401 atcacaagtg ttattgattt tcacaatcat attttaatgg tattatcatt ttgaataatg
     8461 aaaaaacatt aaggatggat gaaatatgaa taacgaaact atcattttag gaaaaaaata
     8521 tatttgccaa cctattggct tgaaacaaaa agtggttgga gaagtaatca gtaaattaga
     8581 aaattgtgtt gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg aaatattaga
     8641 aaaatgcgga aaagtagtgg caaaatatac tcacgtttat gagttggaaa caacagaata
     8701 tctatataac tatagcaaca aatatgaaca tactttagtt tcataaagat ttaaataaag
     8761 tgccttcaaa agcgagaaga ccaagctttt gaaggcactt tatttagttg gcagtgattg
     8821 ttcatgattt ttgatttatt caaaaggttc gcctagagaa tctgtctctg taacgaggcc
     8881 tggtggagaa actcgataaa aacctatact gccagaactg tcctattttc ggatagacaa
     8941 actagtagtt ttaaaaagat catccgatcc aatcatttca aaaaatgcaa aactgatatc
     9001 aggatcattt agctcaggct tgttatcgta aagcatttgt aaaggttgca tttagttagg
     9061 agtaaacgcg aaactatcat cattatttgc tgaagaagat gaagaagcta attcagagtt
     9121 tgtttgaaat aaaggagacg tgctgacagt ttctttaccc tgttttttgc agaggtagaa
     9181 acttttatat cggaagtttc taccggagaa tttttctcat ttgtagaaat tgttgaatcg
     9241 atactattcg aaatttgcgt atctgaaggt atgttctttg atgcagaagt cccacaagca
     9301 gtaagtaaca ataaatttga taaactgata aacagaattc ttttcattat gtacacactc
     9361 cttatagtat cgtttagcat acacctatgc tcagtattag gcaaaatcaa tgcaaaacta
     9421 tcaagtcttt tatagtatct attagtctta gtaatacata tatggagagg gtgatatccc
     9481 agttcaaacc agatacattg gttaccatct gtattaacga ttatgcctct aaacgctttt
     9541 tttatgaaaa aatttataag tgaaaacgta ggtatgatat tatctagagg taattttttt
     9601 gggtatttaa atagttgcta ggaggatagc ttttggattt ttacagtaat tttatcttaa
     9661 taatagcaat attattactt ctaaatattt ggttttttga taagagtaga aatgcaggga
     9721 taggttttag aaccaaacga agtacgtctt ctgaaaaaaa gtgggtatat tcacagacta
     9781 ttttttacgg aggggttatt tcaatcagtt tactttcttc aacactttat tcttttaatg
     9841 ttatagacgt ttccatgtca aattttatat ctattattgg aatccttatt tctgcaatta
     9901 ttacacaact acttcttgtg tttgaagaga agtcaaaaaa taattaaagc aattatgcct
     9961 tttagtacag gaattattac ttcttacaaa tgtatagcga atgtgaagcc tgctgtttta
    10021 attgttctag aaaaactttt gagtgaacaa ttattttatg ttaggatgaa ttaatttctc
    10081 tagagtttat aattaaaaaa cttatttgaa ggaacatgct taaaccataa ttgtgttttt
    10141 ccttccatca gcataaaagt tcacctcaaa aagagtgtac gaacaaaagt tctttttgta
    10201 aaccttaaaa tagattttcc tgaatacgta ttggggattt ctaaatgttt gaatgttttt
    10261 ttgaatgcaa aatcaatgca aaaaattttg tatttctcta cttttctctc cttgaccaaa
    10321 ataaaaaaac gttgatttaa caacgttttt catacatgtt tatctgtctc aaccatacat
    10381 atatggagac ggcgggagtc gaacccgcgt ccaaacacat cgccacttaa aaatctacgt
    10441 tcatagtcaa ctcatttaag gattcacttt acagcttgcc gagtgacagg cattctgtat
    10501 cgctagtctg atgttctctt tttcaactta cagacggaaa gttgaaacgt atcccactaa
    10561 agttaggacc cttactcgag cacatgggcg atgccgagag gatctacgct aactgttttt
    10621 aggcagctaa agcgtaagag ttgttttcgt ttttagcagt tatatttaac tgtaacgttt
    10681 ttacgtagac gtaacctacg aaacgcaatt caagctcgac tgtgcctgtc gaatccgtaa
    10741 cgtccccgtt agctaacaag ggttacgtgt atttttcata caaaattagt atagcatata
    10801 ttgggataaa ttcaataaaa aagcgaatca tatcccatta tttatataat aactgcccct
    10861 ttcttaagaa aaaataagcg attttcttaa gaaaagaggt agcttcaaaa aaagatctta
    10921 aatcagatca aaatacttca atgcgtggac aattccgcct tctgtatttt tctttgtgat
    10981 aaaggtagaa agttctttta atccgtcata ggcgttgccc atagcgattt tgttgtcgca
    11041 agcttccaac aaggcaaaat cattcggacc atcgccaaat ccaaaagtag gaactttttc
    11101 cagctgaagt tctttttgca gacgtttgac acctgcccct tttgagattc ctttcttcac
    11161 tgtatcgatc gagtaaggac cattccgata aaaagttagc tcaggaaaat gttcgtgata
    11221 gtattcgtca cctgattgag ataagatcag caacatattt actgtgtttt ctttaaagcc
    11281 taaaggatca atttccggaa catctgaatg aatgaagtcg taagcattga tcattgtttg
    11341 gctatgaccg gtacaccaga ttttctgatc gttatagtaa gaaagttcat gaccgttcaa
    11401 ctgtacatga tcatacattt tttgacattc ttctgaagaa aatgtttgag aatagatttc
    11461 ttcgccgtct actcgaatga aagaaccatt catggtgacc gcagaagtaa tgcccgcatc
    11521 ttccatgata gagtggattt ctgcttctgt acgtcctgta gcgatgacgg ggagaacatt
    11581 gttttctttt aatgcgttca tagcttttgc cacttcgggt gtgatttttg atttcgcatc
    11641 tagtaatgtt ccatcaagat caaagaaagt gattgcacga taatttgaca caaataaaac
    11701 tcctttttta ttggaataag tttatcttaa caaagaatga acaaaagaca aacctttcgt
    11761 gaagaattcc tgaacatatt ggttcagtca acaatttaga gtatactata agaagagaat
    11821 ttaggaggga aagaatgatt tttttcagta ttggtatctt attcttaata attggcagtc
    11881 tttttcggat tctgccctca aaaggaaatc tgccttttta tgggtatcat tcgcctcttg
    11941 ccgcaaaaac agacgctcat tggcggctgg ctcaaaagac aagcggaaac tggttttttc
    12001 ttatgggctt actgatggca ttgatcggct attatttaaa gacatctggg catacgaatt
    12061 attttctgat tgaaatgctt ctgctagtat ttccaattat gcctattttt attatgacag
    12121 aaaagaaatt acagaaatat gacctggaaa ctggagggaa cgataatgaa tattttaatg
    12181 attgaagata atcaatctgt ttctgaaatg atgcagatgt ttttcttaaa tgaaggttgg
    12241 gaagcaactt tcaagtatga tggaaaagaa gggctcgatg cctttttaga aaacccacaa
    12301 cattgggata tgattacact agacctgaat ttgcccacac tggacggtat gctagtagga
    12361 agagagatcc gcaaagtttc aagtactgtg ccgattatca tgctgacagc tagagattct
    12421 gaaagcgatc aggtgatcgg cttagagatg ggggcagatg attatgtcac aaaacctttc
    12481 agtcctctga cgcttattgc tcgaatgaaa gctttgcatc gccgtagtga gttggcagaa
    12541 gcaagagaag aagcagcagt ttcagatgat cattttgata tcgagactag tcatttcaag
    12601 atgaatacga aaacacgaga agcgtatctg gacaaccggc caattgaagg actgacgcct
    12661 aaagagttcg atctattgtt tactttagca aaaaaaccac gtcaagtatt ctctcgggag
    12721 cagttgcttg aattagtctg ggattatcag tactttggtg atgaacggac agtggacgcc
    12781 catatcaaga aattacgaca aaaaatcgaa aaggtcggac cacaagtcat ccaaactgtt
    12841 tggggagtcg gctacaaatt cgatgattct ggtgttgcct aatgcgttat ttgtatcagc
    12901 aactcttagc tttttgcgga atggtcgcgt tgatcttatt gatcgttgga gtttctttta
    12961 cacaactgac aaaacaaacg attgaagaaa acaattacca gcaactgttt ggatatgctg
    13021 aatcagttga aaagacaacg caaacctatg ccgatacgct cccgcagttt agtcaagatc
    13081 aggcgtttca aaatgcattg ctgctgacag agcaagtact gacagaacaa aatgtgaact
    13141 ttatatttat cgataaaaac gaacgtgtca tttatccgac taatacagct ttactgaatt
    13201 ttaccttaac agctgatcaa tggaaaaatc tgaggaatgg acgtcaagaa aaattcacat
    13261 cgaataaaaa tattttagga caagcacagg cgacctctta cgcacttgtg ccatttaatt
    13321 tgaatcatga attctatgga gcacttgtag ttagtcaacc tgcgcgaaat atcgataaca
    13381 gcgttcgtcc aattacactg aacctgttca aaggatttat tttttccagt atcgttgcag
    13441 ttattgccag ctatgctttt gcagcattcc aagttaagcg tatcaatcgg ttaaggaatg
    13501 cgacgaaaga agtgacgaat ggtaattttg atgtacagct ccccgtacat gacaaagatg
    13561 aatttgatga gttagcagat gatttcaata aaatgacgaa ttcattgaaa gaatctcaag
    13621 cagaaattga agaacaagaa aatcgtcgaa gacaatttat ggcagatgct tctcacgaga
    13681 tgagaacacc tcttacgaca atcaacggac ttctggaagg gttagaatac aatgcaatcc
    13741 ctgaaaatca gcgagaaaac gcgattaaat tgatgaaaaa tgaaacagag cgtctgatcc
    13801 gtttggtgaa tgaaaacttg gattatgaaa aaatccgtac gaatcaaatc agtatggtca
    13861 ttaaaaaatt caatggaaca gaaacattga aaaatattgt tgcacagtta gaagctaaag
    13921 cagaagcagc aggagataca ttgacattga aagccgttga tgatattgat gtttacgctg
    13981 attatgaccg atttgtccaa atcatggtca atattattca aaacgcgata cagtttactg
    14041 aaaacggcca aatcacgatt actttagaaa aaggctatct tgaaacgatc atcacgattg
    14101 aagatacggg aattggaatg tctgaacaac aaatgaaaag tatctgggat cgttattata
    14161 aagtagatcc ttcaagaaaa aatacaaaat atggtgaatc aggattaggt ttaccaatcg
    14221 tgcagcaact tgtacgcttg cataaaggaa aattagaagt agaaagcgaa ctaggaaaag
    14281 gaacaaaatt cgctgttcgt ttgcctgacg tagaaatcaa aaaagagtaa aaaacaagcg
    14341 gtataaaaga tcatgaatcg atcttctata ccgcttgttt ttactataaa atgattgatt
    14401 tagcccgcgg gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta
    14461 aagagtaaga taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct
    14521 tggatctcca atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg
    14581 gaaagcaagg catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc
    14641 gcacaattaa gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt
    14701 ttgatttctt tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac
    14761 atcggccaat ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt
    14821 ttcaagtgct ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt
    14881 tcagcatgaa tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg
    14941 gataaaaaaa gtgaaaggga atagactaag aatgagacag catccactga gcccaataag
    15001 agtttgggca aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt
    15061 aaaaaaagtc tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg
    15121 gatatctggc agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc
    15181 ataaactgac tggaagctta cgatccacaa taaaaccaag atacaacaaa gaaagagagg
    15241 aaagaacagc tgccagaaaa taaaaaggaa taatttggtt ttagaaagac cagtcaggcg
    15301 ccaattgatc aattcctgct tgttttgcct gactgaatag taaaagaaaa agataaaaag
    15361 gaaggcaaat agaaaaaata aactgaatat gccttttata taaaatcggc tagctaattc
    15421 taaagccgga agaagctctg tagatgcaga agcgccaata aatgaaagcc gatcttggac
    15481 ttgattggct aaagccgatt gcaaatggta aagagtcagg catgctatca ataaaaaagc
    15541 agaaaagctt gaaaataaac aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc
    15601 agtagcatag cggattattt tcatactttt cacctctata aaaagtatag aaaatgaata
    15661 tgaataaagt atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa atattttgta
    15721 aaaagcaaga ggttgtgaag aagctgccct attctattac cgtagatgca agcttttgaa
    15781 cactgtttat tcgaaaagag gagcagcgac aataattttg tcgctgctcc ttttaaattt
    15841 attttgtcat tcggaagaac ttgtggtcgt tccgtgaagt tcttggtacg tttgcgtttt
    15901 ataaagatca acagttgatt gtccgttgtg ttcactaaag acactagttg acttgtcacc
    15961 aagagatttt tcagcttctt ttaatttctc taagccattc ttgtagtcat aatcactggt
    16021 atcaattggg tctaatccac tattcgtata gaaccgaaga agatcaccat tattgatttg
    16081 gtcagaaaca gctaactgtt tatcaacttt ttctttcagt gaatcaattt cattctgtac
    16141 ttcctcagta ggttcagtaa tagcttcacc agtttcgtta ctatagatcg taccaccgta
    16201 gtaagtatat tttggtgtaa caaaatcacc atcacggaaa gaaacaagct gttcatgatc
    16261 tttcgagaat agatcttgtc ctaactgaat ataatttttt gtatctacac ctagtaagtg
    16321 aagtaatgta ggcaaggcat caacttgtcc gccgtaagtg tgattgattc cgccctgtgt
    16381 ttgtccaggg atatggatca tataagggac acgctgcatt tgtgcattgt cgtagtttgt
    16441 ccaagtagaa cttgtttttc caactaattc agctagattt ttatttcttg aattcgagac
    16501 accgtaatgg tcaccgtaaa ggacgatgac tgaattatca tacaagccgc tttctttcaa
    16561 ataattgaag aattcttgta cggcggtatc taaataattg gctgtcgcaa agtaaccgtt
    16621 gatcgtttca tcatttgtat tggctaacgg gaatcctgct tcgtcattgg tgaattttga
    16681 ataaggataa tggttagata cagcaatgaa ttttgaataa aatggttgtt gcagacgttc
    16741 caagtattgt acggattgtt ggaaaaatgg tttatcatgc aacccgtatt ggaacgaatt
    16801 gtcatcatta acatcataat aagaagaatc aaagaaataa tcatatccta aattcttgta
    16861 tgtctcgttt cgattccaga atgtaccagc gttcccatgg aataccgcac tagtgtaacc
    16921 ttgtgtttga cttaaaatat caggtgcagc ttggaaagta ttttttccgc ctaattgtgt
    16981 gaacaaggag ccttgattca agccaaacaa tgaattttcc atcagcgttt ccgcatcgct
    17041 tgtttttcct gcttttactt gatggaaaaa gttgtcaaaa ctgaacgtgc tgttgctgtg
    17101 gtaaagacta ttgatgaaag gtgtaacttc atgctggaga ccattctcat ctgtcaattt
    17161 ataatcgatc aggaattgct ggaagctttc taaatggata taaatcacat tttttccttt
    17221 agcgagcccg aacaaatcgt catttggtgc tgcataatgt tcttttacat aattttctac
    17281 ttctttcata tcattgggac ttgcttctgc ccgcacttgg tttgtctggt aagtttgaac
    17341 accgtcataa accatgaaag cattcaagcc aagatatttg accaaataat cacgagagaa
    17401 tgttcgagtc agcaattctg gacgatcagc ttccgccata aaaagatttc ctgagaaaat
    17461 cataactgct aatgtagaga tcgcaaaagc cattcttgct cggatcgggc gagggtccaa
    17521 cttgattttt tttgcaatca gcagaacagt caagataata aaatcgatcc aatagatcac
    17581 atcatatggg cgaaacagtc gaatggcact ttctcccaga ccactcgcta ctttcccagc
    17641 acctaaaatc gtgttgatcg taatgaagtc cgtgaattct cgataataag tgacattcga
    17701 aaaaacaagc aatgtcaaaa gaaaataaat gaccatcatc gtgatataag caactctagg
    17761 gcgtctgata taaagagcaa ttccaagtaa taacaatgta gttgcaatag ggttgataaa
    17821 caagataaag tattgaatgc cactttcgat tccgagatgg aaatcaacta aataggcaaa
    17881 aatcgttttc aaccaaagta aaacgactag taaagaaaaa aatccgagcc tcttattcaa
    17941 aaaggcaggt gtttttatac atttcaagat aatcttcctt tctgcacttc ttaatcacag
    18001 aagtgtcatg aaaccatgtt acacagctca cttattctca tttattttac tagtatgaca
    18061 atatagtgtc aattgaaacc agaagtttat gctgtttcat taaggttttc tttaagcttc
    18121 atgaaaaaat gagcagtaag acaagtccta tgttttttgt tatacttggg aagagaaagc
    18181 taaagcaggt gaaggaatga aagtaaaagt aaaaaaaaga gcaagcaatc gattgaaaaa
    18241 aggatatcca ctgatccaaa aagaagacct tgctcatccc caaaaagaaa ccacgcaatg
    18301 ggtggaatta gttgattcgc aaaaccaatt tatcgcaaaa ggatatctag gaaagcagaa
    18361 taagggaatc ggttgggtat tgagccagca gaacatccca tttgatcaat ctttttttga
    18421 agaatttttt gtgaagtcaa aagaaaaaag aactttcttt ttttcagatg attctactac
    18481 cgcttttcgt atattcaatg gagaaggaga cggaatcggc ggtctgattg ttgattatta
    18541 tgcaggttat gctgtttttt cttggtataa cgaaacgtta tatgcacatc gtgaattatt
    18601 gattcaagct tttcgtgaaa tctacccgga aatcgtagga ggctatgaga aaattcgatt
    18661 tgaatcagta aatctgcctg agagccagtt catttacgga aaagaggcac cggaaccctt
    18721 gatcgtaaaa gaaaacggag taaaatttgc cgtttattta aatgaaggat taatgactgg
    18781 gatcttttta gaccaaaaag aagtacgggg atcattagca gaaggtttcg cttctgggaa
    18841 aacagttttg aacatgttta gttataccgg cgccttttct gttgctgccg caatgggagg
    18901 ggcaatcgaa acaacaagtg tcgatctagc aaaacgcagt ttaaaaaaaa cacaggaaca
    18961 gtttgaagtg aatggtttgg atgtcgatca gcaaaaaatc attgttatgg acgtattcga
    19021 gtatttcaaa tacgccaaaa gaaaacaatt tttatatgac atgattgtat tggatccacc
    19081 aagttttgcc agaaataaga aaaaagtctt tcgcgtagca aaagattatg gggaactggt
    19141 caaggatagt ctggatattt tagcagaaga cgggatactt attgcgtcta caaatgctgc
    19201 gaatgtctcg atagaacaat tccaaacaat gattgaagag gaattccatg ccaaaaatgt
    19261 ttcttttgaa caacttgctt tatatcgtct gccaaaagat ttccagacga cggatacctt
    19321 tttagaaggc aattatctga aagtgtttgt ttatcaaatt aaaaataacc attagaagga
    19381 gcgatgcaga atggtttggg taaagggatt gaatatagga agcggtagac caaagatttg
    19441 tgcgcctctt atcggggccg tccgagagga agtattacgt gaagcagttc ttgcacgtga
    19501 agcaggggcc gatttagtcg aatggcgagt cgatcattat cgtgaagttt tttcttttga
    19561 tgaagtagca gaaacattgg ttcttatcca tgagatgctt gatggactgc cgttattatt
    19621 tacgtttcgt accttagctg aaggcggaga acaagacata tcgatccgtc agtatcgcga
    19681 cctttatgaa catgtgatac acacaggatt agtagatatg gtcgatatcg agttgttcaa
    19741 agtagaaagt ctaggaaaaa gactgataga ggaaatcaag tctttaaaca ttcctttaat
    19801 tatcagtagt catgatttta aagaaacacc agcagaccct gtcttgcttt accgattgaa
    19861 tgttatggaa cattttggag ccgatatcgg aaaactggca gtaactccca ataatgaacg
    19921 agatgttttg cggttgatgg agttgactag acgggcaaat gcttttgtct ccatgcctat
    19981 tattacgatg tccatgggaa atcttggtaa aatttctcgt ctggctggcg gaacgacggg
    20041 ttccgttatg acatttggtt ccttaagcag cgattctact tctgctccag gacaattacc
    20101 ggtcaaagca ttgaagcaaa ttattcaatt attaaatgaa ccgttgtaga acaagtttga
    20161 ggtgatattt tttggcaaag aaaaaaatag taaaaacgaa tgcaatccgt atggtcgaac
    20221 aaaaaaagat tccctataca gagcacgaat atgagtggga cgaaagccac ctaagtgcgt
    20281 catcggtcgc tgaacagctt ccagaaagcc aatctcgtat atttaaaaca ctggttgctg
    20341 tgggcaatgt gacggggcct cttgttgctg tgataccggg agaagccgaa ttgaatctaa
    20401 aaaaattagc aaaagtttca gggaataaaa aagtagaaat gctccattta aaagatttgg
    20461 aagcaacgac cggatacata cgtggtggct gctcgcctat aggtatgaaa aaactatttc
    20521 ctacttattt agatcaaatt gcagaaagct atgaacaaat cattgtctcg gctggcagaa
    20581 gagggctaca gatggagtta gcacctcaag atatctgtgc attaacgagc ggacaatttg
    20641 cagatataaa acaataaatc atacgtataa aagaaagaag gaggccggga tgttgtttgt
    20701 ccagtctcct tctttctttt ttatacatta tgctctttgc ccggcattca ttcctgctac
    20761 atgccctgta gcgaaagccg ctgtgatatt aaagccgcca gtataaccat tgacatccag
    20821 taattctccc gcaaaataca gaccatcaat gattttactc tccaatgttt taggataaat
    20881 ttcctttaga gaaacccctc cgccagttac aaatgatttc tcaataggaa aagttttgtt
    20941 gattgatagc ttaaactctt tgcatagttg aacaaaatct tgaatttgtt tttgagaggc
    21001 ttgttgtcct gtcagatgat ccatttcaag ccgctcaaga aagaaaacta acaatcgctc
    21061 aggcaatata ccatgccaag cattgaccaa gttctttttg gttgctttac ttttttctgt
    21121 caatatatga ataagctctt gtttggtttg tgtagggaaa caatccagag aaagtgtcac
    21181 tggttctccc gttttctcta attctttatt gacaaaactt gaacaacgca gtgcagccgg
    21241 accagaaata ccgaaatggg tgaaaagtaa atccatcgta tgttcagtca atacacgacc
    21301 ttttttattt aaaacgcgta aagtgatgtc ttgtaaagaa agcccttgaa gtgttttctc
    21361 ctgaatatac ggttcatccg atatcaaggg ggactctgtc gcataaagag gcgtcaccgt
    21421 gtgacccact cgcttcacga tcttgtaccc atctccagta gaaccagtag atggataagt
    21481 ccgtccacca gttgttaaga taatggcagg agcatggaac tcttcgaatt ccgtgcgtat
    21541 accgtaaatt ttatgttctt tgtggatcaa tttctctact cgagtagaga atagaagcgt
    21601 cacatctagt tctttcagac gattgagcaa tgcttcgata atcgtttttg atttatttgt
    21661 tacaggaaac atccgtccat gatcctcttc ttttaaatgt acgccttgtg attcaaagaa
    21721 gttcatgata tcgaaattat tccattgcgc aaacgtacta tataaaaact ttccatttcc
    21781 aggaatatgt gcaatcaaat catcaacagg acgattgttc gtaacattgc accgaccacc
    21841 acctgtcatc agtagttttt ttcctgcttt tttgtttttt tcgatcaaga gtacgcgtgc
    21901 gccttgttca gctgcactga tcgctgccat catcccgctt gttccagcgc cgactacgat
    21961 cacatcatac agtttttcca ttccctcacc tcaaggaaag tgtagcatat ctgaagaaag
    22021 aaaacagaaa aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga gggaagataa
    22081 gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat tcaagaaaag
    22141 ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga attcatgccc
    22201 acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat cttagaactt
    22261 ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga tgaaggaaac
    22321 tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc ttataaaggt
    22381 ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc ttttgaacag
    22441 attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg aagtgacttc
    22501 gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt catgctggaa
    22561 ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg tgtaggagct
    22621 agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga tactggtgtg
    22681 ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc tactggttat
    22741 ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt taatcaaaaa
    22801 acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa agtgcaagag
    22861 ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga tccagaagga
    22921 atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt gacggaatac
    22981 gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac attgaaagaa
    23041 aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag cttggccgaa
    23101 atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc atgtacagta
    23161 aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa agctgctaat
    23221 gctggaggag tagcagtatc tgctttagaa atgagtcaga acgtccagcg attgacttgg
    23281 gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta ccatacgtgt
    23341 cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc aaatgttgct
    23401 ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa aatgaaactt
    23461 tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa aaaaacgtaa
    23521 agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat tcattttgac
    23581 tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca aagccaagta
    23641 acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt ccgcggctgg
    23701 atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga agcagctaaa
    23761 aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc ttatttaggc
    23821 gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga tagcaacaca
    23881 agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata tattgctgac
    23941 ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa atctggaaca
    24001 actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa gaaatatgga
    24061 caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg tgcagttaaa
    24121 gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat cggcggacgt
    24181 ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc agatatcgat
    24241 gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa actagaagaa
    24301 aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg caaagtaact
    24361 gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg gaaacaattg
    24421 tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc aaacttctca
    24481 actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat ttttgaaaca
    24541 gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc agaagattta
    24601 gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa agcatttgaa
    24661 ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa aattccagaa
    24721 atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt aggtatttca
    24781 ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta caagaaaaac
    24841 atgttcgcac ttctaggcaa accaggcttt gaagatcttg ctaaagaatt gaacgaacgc
    24901 ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca tgttcgcttt
    24961 aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta ttcagaataa
    25021 ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga ttgattgcct
    25081 tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa ggttaattct
    25141 gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg cttttttgcg
    25201 atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt accttatcgc
    25261 tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc aagatcgttc
    25321 cttcggggta agcagaacca ttggcagagt caccagaagt aatgacatcg aaatctaact
    25381 gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct cttccaatta
    25441 cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg actaccgcat
    25501 aataatcttt acctacataa gtagactgat agtagtccca acctttccat cctataaaag
    25561 caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc cttttttatt
    25621 tttatta
//
LOCUS       DARBFD010000030        25625 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000030 DARBFD010000000
VERSION     DARBFD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.030"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            133..3660
                     /locus_tag="SCK08_001218"
     CDS             133..3660
                     /locus_tag="SCK08_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEG1201121.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="SCK08_001219"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="SCK08_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEG1201122.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="SCK08_001220"
     CDS             7448..7789
                     /locus_tag="SCK08_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1201123.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="SCK08_001221"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="SCK08_001221"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEG1201124.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="SCK08_001222"
     CDS             9146..11566
                     /locus_tag="SCK08_001222"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEG1201125.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="SCK08_001223"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="SCK08_001223"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEG1201126.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="SCK08_001224"
     CDS             13000..13737
                     /locus_tag="SCK08_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1201127.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="SCK08_001225"
     CDS             13751..14566
                     /locus_tag="SCK08_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1201128.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="SCK08_001226"
     CDS             14579..15226
                     /locus_tag="SCK08_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1201129.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="SCK08_001227"
     CDS             15242..15898
                     /locus_tag="SCK08_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1201130.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="SCK08_001228"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="SCK08_001228"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEG1201131.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="SCK08_001229"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="SCK08_001229"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEG1201132.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="SCK08_001230"
     CDS             18240..18758
                     /locus_tag="SCK08_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1201133.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="SCK08_001231"
     CDS             18865..19365
                     /locus_tag="SCK08_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1201134.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="SCK08_001232"
     CDS             complement(19645..20565)
                     /locus_tag="SCK08_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEG1201135.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="SCK08_001233"
     CDS             20730..21743
                     /locus_tag="SCK08_001233"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEG1201136.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="SCK08_001234"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="SCK08_001234"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEG1201137.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="SCK08_001235"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="SCK08_001235"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1201138.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="SCK08_001236"
     CDS             complement(23383..24144)
                     /locus_tag="SCK08_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1201139.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="SCK08_001237"
     CDS             24333..25427
                     /locus_tag="SCK08_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1201140.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>25625)
                     /locus_tag="SCK08_001238"
     CDS             complement(25513..>25625)
                     /locus_tag="SCK08_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1201141.1"
                     /translation="SVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8485 a         4439 c         5406 g         7295 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actgg
//
LOCUS       DARBFD010000031        25269 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000031 DARBFD010000000
VERSION     DARBFD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.031"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..258)
                     /locus_tag="SCK08_001239"
     CDS             complement(<1..258)
                     /locus_tag="SCK08_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201142.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="SCK08_001240"
     CDS             complement(274..924)
                     /locus_tag="SCK08_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201143.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="SCK08_001241"
     CDS             complement(935..2107)
                     /locus_tag="SCK08_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEG1201144.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="SCK08_001242"
     CDS             complement(2126..2998)
                     /locus_tag="SCK08_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1201145.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="SCK08_001243"
     CDS             complement(3020..3940)
                     /locus_tag="SCK08_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201146.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="SCK08_001244"
     CDS             complement(3940..4209)
                     /locus_tag="SCK08_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201147.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="SCK08_001245"
     CDS             complement(4206..4586)
                     /locus_tag="SCK08_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEG1201148.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="SCK08_001246"
     CDS             complement(5053..6516)
                     /locus_tag="SCK08_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG1201149.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="SCK08_001247"
     CDS             complement(6580..9597)
                     /locus_tag="SCK08_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1201150.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="SCK08_001248"
     CDS             complement(9748..10587)
                     /locus_tag="SCK08_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1201151.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="SCK08_001249"
     CDS             10732..10977
                     /locus_tag="SCK08_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201152.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="SCK08_001250"
     CDS             11040..11462
                     /locus_tag="SCK08_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201153.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="SCK08_001251"
     CDS             complement(11556..11873)
                     /locus_tag="SCK08_001251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201154.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="SCK08_001252"
     CDS             complement(11900..12106)
                     /locus_tag="SCK08_001252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201155.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="SCK08_001253"
     CDS             complement(12087..12269)
                     /locus_tag="SCK08_001253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201156.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="SCK08_001254"
     CDS             complement(12283..12633)
                     /locus_tag="SCK08_001254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201157.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="SCK08_001255"
     CDS             complement(12659..12961)
                     /locus_tag="SCK08_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201158.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="SCK08_001256"
     CDS             complement(12964..13197)
                     /locus_tag="SCK08_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201159.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="SCK08_001257"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="SCK08_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="SCK08_001258"
     CDS             complement(14628..15743)
                     /locus_tag="SCK08_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1201160.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="SCK08_001259"
     CDS             complement(15749..16147)
                     /locus_tag="SCK08_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1201161.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17176..17562)
                     /locus_tag="SCK08_001260"
     CDS             complement(17176..17562)
                     /locus_tag="SCK08_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG1201162.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17559..18080)
                     /locus_tag="SCK08_001261"
     CDS             complement(17559..18080)
                     /locus_tag="SCK08_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1201163.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="SCK08_001262"
     CDS             complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="SCK08_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG1201164.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19149..20564)
                     /locus_tag="SCK08_001263"
     CDS             complement(19149..20564)
                     /locus_tag="SCK08_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEG1201165.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20533..22686)
                     /locus_tag="SCK08_001264"
     CDS             complement(20533..22686)
                     /locus_tag="SCK08_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEG1201166.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            22944..23180
                     /locus_tag="SCK08_001265"
     CDS             22944..23180
                     /locus_tag="SCK08_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEG1201167.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            23199..23414
                     /locus_tag="SCK08_001266"
     CDS             23199..23414
                     /locus_tag="SCK08_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEG1201168.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(24117..24869)
                     /locus_tag="SCK08_001267"
     CDS             complement(24117..24869)
                     /locus_tag="SCK08_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1201169.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(24884..>25269)
                     /locus_tag="SCK08_001268"
     CDS             complement(24884..>25269)
                     /locus_tag="SCK08_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1201170.1"
                     /translation="QANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIE
                     SQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEIN
                     KKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT         7201 a         5008 c         3843 g         9217 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    16561 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    16621 aaaaattgac attaatgact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
    16681 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
    16741 cgctgttctt tttttatttt tcaaaataac tagggctttt tatttttaac tcaaagtcca
    16801 ctgtagccct ttggactttg agttaaaaaa gaagcataat tatgaacagt aaagtttcta
    16861 atataacaat aattcgagaa gctttgcaaa atcatgctgc caatgaactc tctaaacata
    16921 taggattaaa tctaagtact ataaaaggtt aaaaagtgaa gaatatttaa tagaaaaact
    16981 aaatttactt gctgctttcc ccttttccat taatacaaca atcagagagt gtcttttttt
    17041 cgaaaaaatt agaataaata aaagacacgt agttttttta tctacatgtc ttccatcgac
    17101 tacaagttat catttctcaa aacgtattta gcttttaatt gattaatcgt ttcacgttgt
    17161 ctacacattg cctaattaag attttcttat tagaagcatc aatcccgcaa taatacctaa
    17221 aactccagga attacccaca ataatgttaa aagaaaaaat ggaataatag ataaaatgag
    17281 taaactaata ccatagacca ctgttttttg attttttctt actaaaaaac ctattatgaa
    17341 aatcattatc gaaaaaacta gggtaattcc tccaaataaa acaacattcc cgcttagatt
    17401 gactaacata tctatatcct tactggttag ttctgtagta tttaaagtag ttgaatcaat
    17461 agaagataga aacataccac ccatcaatgc taaaagacta cataaaatcc ctgttattcc
    17521 actaattaaa atcaaaataa actctgtttt tctcttattc atttaattct ctccagatta
    17581 agtgttttag aattagaaat tttttgacca tagttaatag cttctaatga acgaataaca
    17641 accactgacg tgttagaaaa ataatcccca atatgttgtt tcttgggcgt gaaagcagcc
    17701 accaaatacc caatttgaaa tacaaaattt tttaatataa acctacatat acactcacga
    17761 ataaagatag tttgccaact tagctctttt tcctcaaagc tcactactct tatcccaaaa
    17821 atcattttcc caattgtttg tcctgaattc agcttagtca aaagtacaaa ataggttaaa
    17881 taaatagcta atgacgctat accataaaaa gtaataatgg atttgtcaga ttccagtccc
    17941 atgattttgt aaaagagacc tatagtagca tgggtgatga tgctaataca aattaaatca
    18001 ataagaaaag caaaaaatcg taaccaaaat cccgcaaata cataattagg caaagtttca
    18061 atgccactct tcttattcat tttatagccc tccatagtaa tacattggtt ttggagtatc
    18121 agaaaatcca agtttttcaa aaactgaaat tacacgatct gatgtagttg cctttagccc
    18181 ttgccattca gcaatcttat tacctaacca agaagaagca aagccagttg tgtcattttt
    18241 atattctata accgtcgcat cagttaattt cttctctttt tttagggaat ctaatgcatc
    18301 ttcagagtaa ccaatttgat ccaccaaccc attttctact gcttggttac catcataaat
    18361 tcttccatcc gcaagttctt tcactgcatt agtatccata tttcttcctt ttgctacaac
    18421 attgacaaaa cgatcataag cactcatcac aaattcctgt ataactttat tctcttcctc
    18481 tgaaggtttg tgttgaggtg acatcatatc ttttaatgca ccacttttat acgtcgcatc
    18541 agttacgcca agtttttcta ataagccaga ataatttaaa ccagaaataa tgacaccaat
    18601 tgatccagtt gttgtttctt ccgtagcaaa tattttatct gaaccagcag aaatgtagta
    18661 ccctccactt gccgctgtat ttttaaatgc agtatagata ggaatatcta gctttttaat
    18721 ttttgccatt tctttagcaa tctcagcact ttcatatatt cctcccccag gtgaatttac
    18781 ttctaataac actcccttaa ccgctttgtc ttcttgaatt ttctttagct gtgttaaaaa
    18841 attctgatgg ttatattgtt ccctactaaa aagattgctt tcacctgtat ctgcaataac
    18901 tccattgacg gatagtttga caatcttttt atttgatgcg ccttcttcta acgtttcttc
    18961 tgtgatttcg ttagatccgt aaaaaatctt atttattcct gttagggttg tatttgatgc
    19021 ttcctcttta tcttttcctg gaattagcgc gacgataatg gatagtaaca ataatccgca
    19081 cgctactccc acagcaatcc atcttctttt attcatataa actcctccta tattcttagt
    19141 aaaaataatt attttttatt tagaaacaca tcgacaattt gttgccaata tgttttttct
    19201 cctacaatta aagacaactc tccagttaat ccataacgaa acggaaacga ttgagttgtt
    19261 tttaacgttc ctttcactgc ataaaaaaca cctttctcag tggcagtaga attttctgaa
    19321 atttcatcta aagttccgtc gatcgtaatc ggagccccac ctttttgatc caatttaaaa
    19381 tgaaccttca tcccttcttt aatataggta ctttcattcg caggtaaaag tgctgtaaat
    19441 ttcatttttg atttttcgtg gatcggataa atttctgcaa gcactgttcc tttggctagt
    19501 tctgcttgtc cctttgtttc ttcatttaaa tgaaccgttc cagatacagg agcttttagt
    19561 attccttttg aaagttgatc gtccaaactt ttgataactg actggttctt ctcttgtttt
    19621 tcatttagct ctgtaactgt ttgttttaca gttgttattg cttgttctag ctcttgcttt
    19681 cctttttccg tttgactaat tctgtcattc tcagaagtag ttggtttaac taatttagct
    19741 ttttctgact gatactgttc tacttctttt tttagttgct caatttgttc atttatgctt
    19801 gctgaaattg tcagtttcac ttgatttttt tgatcatcag aaacattatt tagttgttct
    19861 tgccaattct catagtttgc catcatttct tttgaaaaat cttttaatga ttgattatta
    19921 ctccaagcta cctgtacttg ttgccattct tgtaatttac tatctgaact ctcaatttgt
    19981 ttttctagtt gtctttttgt cttttcgtat acttctaatt gcttttgatc atttaattca
    20041 ctttgcttca aagcgtagcg gagtgattct ttttctgaca acatgctctt taattgatta
    20101 ctgtagccaa aactgtcatc cgttgaaaac aaattttttc ctttacttat actttcaata
    20161 aatagctgag ccatctttat ttgttgagta atacgttggt tttcttgttc tatctgagcc
    20221 ttttcattct ctattaaaga acaatccaat ctaaccaaca catcgtcctt atgaacaaat
    20281 tgattttctt ttaggaagtt ttccattatt ttagcttcta atggcacttg taacttctct
    20341 attttttcgg ggttcaattg agccgttgtc cgaataacaa cctctttttt agcaaaggtt
    20401 aaaaacaatc caataataca taaaaaaaga attacagggt ataacaccca acggtagaag
    20461 ctatgatgtt gtttagtata gatactagaa tgttcgaacc aattattatt ctgcataaat
    20521 tgtttccctc tcctagattt cccataattt ttgatagata ccatcggcaa aacgaagttc
    20581 atcgtgcgtt cctttttcta ccaaatgccc ttcatggaga actaaaattt gatcacatgc
    20641 ttttgctatt ggtaaatgat gcgcaataaa aataatcgtt ttttctttta aattgaggag
    20701 ataattcata attgcatgtt ctaacaaggt atctaatcca ctagttgctt catctaaaat
    20761 taatatgtcc ggatttttta ataaagcccg tgctaaggct aatcgttgtc gttgccctcc
    20821 tgaaagatta tttccacctt cttccaacaa tgtctcgtaa cgtaatggct gttgattaat
    20881 aaattcattt agttggacta tctcacaaac ttctattatt tgttcgaaac ttggtgggct
    20941 atctaaaccg aatataagat tctcaatgat tgttccacta aagaaaaatg actcttgagg
    21001 caaatatgtc actcgatcgc gtaatatctg aaaaggaatg tctaagtgat taatgtttcc
    21061 ataatggatc gatccttctg atgcttcata aaaacgaaca aataactttg caagagttga
    21121 ttttccagaa ccactaactc caactatagc cgttttactg tgcgaaggaa ttgtacatga
    21181 aatttcgttc aacgttgggg ccttcatgct atacgagaat gaaacgtttt ttacagtgat
    21241 atcttgttga aataattgtt cagaaagcat atattcaaaa gactggttcg atttttctga
    21301 atcaatcgcg aaaatttcat ttaaacgatt atttgcaact tgagccgttt gcattttcac
    21361 ttgtaaatta ataatgtttt gtaaaggatc tgtcaaaaat actagtaaag cattgtatgt
    21421 gataagttga cctagactaa ttctattatc cataacaaaa taagaaccta gccataaaat
    21481 taatgcactg ctaattaatt gaatcaaatg tttaattcct tgttgaatat tatcaagcgt
    21541 aaccgtacga aatgattttt tcattaattt aacaaattca tggtctatac ggttatatac
    21601 tttctcttca ccgctatatg ctttaattgt ttctattcct ttaagacttt ctatgatact
    21661 agaatttaca gctgcaccgg ctgccatttc ttccctgttc gatttttcat agcttttcac
    21721 gaaagcgaat attgcaacta cataaaaagg caaagagcat agagtgatta aaaacaacgt
    21781 actattctga atagctaacg taaccccaac aagcaacacc ataccaatat ctaaaaatat
    21841 tgataacgta gcacttgcta aagcatcaat aatcttactg gcatcaagaa aacgagaaat
    21901 aatttcccct gattttctcg tagcaaaaaa attcattggt aaagttaaca catgtttgaa
    21961 atactgaagc ataattgcca tactcattcg ctgtcctaaa ataataagaa gataactacg
    22021 actatattca aacaataccc gaaataaata gacagcgatc aagccaatgg aaattatatt
    22081 taaagttgaa cgtgcttgat tagggatgaa aaaatcaaga ataccttgaa aataatatga
    22141 gcttcctatg ccaaaaaacg taatgaaaat cgatgctaaa acgatgtgga atactaatcc
    22201 tttttgtttc caaattatag gcaaaaaaga acttaatcca gctacttttt ctttaatagg
    22261 aatataagtt tctctaggca taggtaataa aagaatacct gtccattctt tttgaaacgc
    22321 ttcaatcgtt tttctatttt ttccattagc gggatctgca atccatagta cgtttccctt
    22381 cgttttataa attacaacgt agtgcatata gctgttatca accaatatat gtgcaattaa
    22441 tggtaagata agatctcttt ctttccagat ctcactatct gcttgaatag ccatacaatc
    22501 aaaagacaat ttttcaaatg tctttttcaa tccaaatgct gaagtaccct ctaaatcagt
    22561 cccagatagg tcccgtaatt tatgaatcgg gatatcagtt ttaaaatatt ttaaaatcat
    22621 cgaaaggcaa gcaacaccac aatctttttc atcttgttgc tgcacaaaag gaaatgattt
    22681 tatcacttat ctaacacaac tccttctaaa aaatttttat agaaaaattc tcaatatagc
    22741 tttaatacag tgaaaatatt gtctcataat tccacaaaaa ctttgaaaac agttcttgaa
    22801 cactaacttt ttcattcctg aatcaaattt attttggaaa ttttactatt caagaataaa
    22861 cttgataagt ttcaggaaga tttttactgt taatcaaata cgtatgttac agtagcaccg
    22921 taaaaaatat tatttaaaga agcgtgttta aacatgcaaa atgtaaagga actaagtatt
    22981 gtggaaatgc acaaactgtt ggtggaggca ataatgcttg gggaaaattg gggcaagttg
    23041 taggtggact taccacaggg gcagtaggtg gtgcaggttt aggaacagca atttgcggac
    23101 cagcttgcgg tgttgttgga gggttatatg gagctgtcgc tggaggtgct gcagctggct
    23161 gggatgctcg aaaaaaataa aaaaggagaa taagtagtat gcatattaaa aacacaaaaa
    23221 caacatttat attatcttca gaagagttga aaaatattca aggtgggagt gccgtgggag
    23281 ttttaggcac tactttttct ggggcaacag caggagtaaa actttgttca gccggaggac
    23341 cttatgcaat agctgcttgt ggtgtaggtg gagctttatt aggtgctgga ttcagcatgt
    23401 ggaccggtgc gtaaacaatt aaaaaatatc aaacctcaag attggtttgt gtcgattacg
    23461 aacataggta tgttctttat cttttcaaaa gtttggaatg gacagaactt tttactaaag
    23521 cttcttgtgc tagtttttat aattatatta aatttttatc ttttattaaa gtcagataaa
    23581 aattctttgg aaatgtaatg gtagagatat ttttacaaaa ggacttgcag cgctgtagtt
    23641 ggtgctgcag gaggacctat ttcggccgtt acaggaggta ttggtaatgt acttgtcgga
    23701 tgtgcattaa agcctgttcc tgcaagttaa atttataaaa acaggaaagt aattatctca
    23761 aatcctatca agtacaatca taggattgca cttgacagga caaatttttt aactaaaaaa
    23821 tgaaaaacac tttctaaaaa ttagtagaat ttgccaaatc tttcgcatct tccaccatac
    23881 atttaatttt aattcccctt tatggtactt aataaaaaca agaaaagaac tattcatttg
    23941 gccctttttt ggttccagac catagaaata ttcctccagt aactaatatt gctgttatgc
    24001 acgtaactaa taacagctat aaaaattggt acaactttaa taggattcag aaaaatttgc
    24061 actagtgaag gaatctattt tattagaaaa atgcaatctc cctaaagaaa aaaccactaa
    24121 acagtgcttt ctttcaactt ctccttaatt ttttgtgctt tccctaaagt aaagtttctg
    24181 gccaactctg aatcaaaata tagtgaacgt tttttaaaat ctatttcttt aacgttaaag
    24241 atatttacta aacacgctcg actaattctt gttaacatag gatatttctt ttcaagctca
    24301 ctaattcttc cataaaattc gtactgccca tctttagtac acaatgataa tctatgtggt
    24361 aattttgaag cttctacaaa ataaatgtca tttatatcaa aagtaaatgt ttgagaacct
    24421 atggaaaaaa caaatgcttg attcgtaacc tgttttgttg catctatcct ttcttgcgct
    24481 aataacaata actcaacaat ctcgtttcga tactcatcta gtgtttgatc ttttgttaca
    24541 aatcctaacg tttcaactct tctttttatt gttacgggta acatctcatc atgagtagtt
    24601 gtaaaaatga tttttgcttg aacatcatat tttctgatta cttctgctaa ttctataccg
    24661 ttaacttcat gatttaaatc aatatctaag aaatatattc cttgctttgg tcggaattgt
    24721 ttgaggtatt ttttaacttc taacggactt tgtgttttca atactatctt aaatacttta
    24781 tcgtgaaaaa gaatgaaatt atcaataatt cgttctaact gatttaattg tataatttga
    24841 tcttcacata atattattgg ataactcata aatcctcctt ccctcaaaac aacaaaataa
    24901 cttgagtagt aaattgatac ggatcgtttt gatagttcac aaacatattg ggattctttt
    24961 tattgatttc ctctatacta gataaaccta acccttgatg tccctttttt gttgttattc
    25021 cttttttcat tagagtatct atagagagta gtatctctct acaactattc gatatcgaaa
    25081 tttctatctg tttatcatct cggtaaatag ctagagacag ttctctatct tgactctcta
    25141 tagatgcttc aattgcatta tcgagaataa ttccaagcac cctaatacag tcaaatattg
    25201 gaacaggaac ttcaataata gtacttggac attcgaattt acaacaaatc ttattgttat
    25261 ttgcttggt
//
LOCUS       DARBFD010000032        24723 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000032 DARBFD010000000
VERSION     DARBFD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.032"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..81
                     /locus_tag="SCK08_001269"
     tRNA            8..81
                     /locus_tag="SCK08_001269"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="SCK08_001270"
     tRNA            101..173
                     /locus_tag="SCK08_001270"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="SCK08_001271"
     tRNA            201..274
                     /locus_tag="SCK08_001271"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="SCK08_001272"
     tRNA            288..361
                     /locus_tag="SCK08_001272"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="SCK08_001273"
     tRNA            385..474
                     /locus_tag="SCK08_001273"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="SCK08_001274"
     tRNA            487..560
                     /locus_tag="SCK08_001274"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="SCK08_001275"
     tRNA            579..654
                     /locus_tag="SCK08_001275"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="SCK08_001276"
     tRNA            656..726
                     /locus_tag="SCK08_001276"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="SCK08_001277"
     tRNA            740..813
                     /locus_tag="SCK08_001277"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="SCK08_001278"
     tRNA            838..925
                     /locus_tag="SCK08_001278"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="SCK08_001279"
     tRNA            947..1018
                     /locus_tag="SCK08_001279"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="SCK08_001280"
     tRNA            1041..1130
                     /locus_tag="SCK08_001280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="SCK08_001281"
     tRNA            1143..1216
                     /locus_tag="SCK08_001281"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="SCK08_001282"
     tRNA            1235..1307
                     /locus_tag="SCK08_001282"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="SCK08_001283"
     tRNA            1311..1383
                     /locus_tag="SCK08_001283"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="SCK08_001284"
     tRNA            1388..1458
                     /locus_tag="SCK08_001284"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="SCK08_001285"
     tRNA            1471..1544
                     /locus_tag="SCK08_001285"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="SCK08_001286"
     tRNA            1556..1627
                     /locus_tag="SCK08_001286"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /locus_tag="SCK08_001287"
     CDS             1893..3086
                     /locus_tag="SCK08_001287"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEG1201171.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="SCK08_001288"
     CDS             3216..4688
                     /locus_tag="SCK08_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1201172.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="SCK08_001289"
     CDS             4771..5559
                     /locus_tag="SCK08_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEG1201173.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="SCK08_001290"
     CDS             complement(5626..6294)
                     /locus_tag="SCK08_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEG1201174.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="SCK08_001291"
     CDS             6409..8121
                     /locus_tag="SCK08_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201175.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="SCK08_001292"
     CDS             8124..9893
                     /locus_tag="SCK08_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201176.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="SCK08_001293"
     CDS             complement(10098..10664)
                     /locus_tag="SCK08_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1201177.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="SCK08_001294"
     CDS             complement(10664..11620)
                     /locus_tag="SCK08_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEG1201178.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="SCK08_001295"
     CDS             complement(11620..12276)
                     /locus_tag="SCK08_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEG1201179.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="SCK08_001296"
     CDS             12646..14664
                     /locus_tag="SCK08_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1201180.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    14734..14940
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15091..17505
                     /locus_tag="SCK08_001297"
     CDS             15091..17505
                     /locus_tag="SCK08_001297"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEG1201181.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="SCK08_001298"
     CDS             17853..19406
                     /locus_tag="SCK08_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1201182.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="SCK08_001299"
     CDS             complement(19787..21571)
                     /locus_tag="SCK08_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201183.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="SCK08_001300"
     CDS             complement(21586..23307)
                     /locus_tag="SCK08_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201184.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="SCK08_001301"
     CDS             23407..24168
                     /locus_tag="SCK08_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1201185.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7709 a         4508 c         5204 g         7302 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    24661 aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag aaaaatcaca
    24721 aag
//
LOCUS       DARBFD010000033        24575 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000033 DARBFD010000000
VERSION     DARBFD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.033"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(152..550)
                     /locus_tag="SCK08_001302"
     CDS             complement(152..550)
                     /locus_tag="SCK08_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEG1201186.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="SCK08_001303"
     CDS             complement(547..1320)
                     /locus_tag="SCK08_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1201187.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="SCK08_001304"
     CDS             complement(1317..2000)
                     /locus_tag="SCK08_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1201188.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="SCK08_001305"
     CDS             complement(2167..3054)
                     /locus_tag="SCK08_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1201189.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="SCK08_001306"
     CDS             complement(3051..3920)
                     /locus_tag="SCK08_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1201190.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="SCK08_001307"
     CDS             complement(4057..5343)
                     /locus_tag="SCK08_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1201191.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="SCK08_001308"
     CDS             5616..6194
                     /locus_tag="SCK08_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1201192.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="SCK08_001309"
     CDS             6194..7123
                     /locus_tag="SCK08_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1201193.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="SCK08_001310"
     CDS             7120..8856
                     /locus_tag="SCK08_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1201194.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SCK08_001311"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SCK08_001311"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEG1201195.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="SCK08_001312"
     CDS             complement(9752..10261)
                     /locus_tag="SCK08_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEG1201196.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SCK08_001313"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SCK08_001313"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEG1201197.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="SCK08_001314"
     CDS             complement(11562..12653)
                     /locus_tag="SCK08_001314"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEG1201198.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SCK08_001315"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SCK08_001315"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEG1201199.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SCK08_001316"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SCK08_001316"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEG1201200.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SCK08_001317"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SCK08_001317"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEG1201201.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="SCK08_001318"
     CDS             complement(16616..17590)
                     /locus_tag="SCK08_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEG1201202.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="SCK08_001319"
     CDS             complement(17571..19454)
                     /locus_tag="SCK08_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEG1201203.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="SCK08_001320"
     CDS             complement(19472..21697)
                     /locus_tag="SCK08_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1201204.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="SCK08_001321"
     CDS             21812..22846
                     /locus_tag="SCK08_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1201205.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="SCK08_001322"
     CDS             complement(22913..23926)
                     /locus_tag="SCK08_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1201206.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="SCK08_001323"
     CDS             complement(24004..24192)
                     /locus_tag="SCK08_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEG1201207.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc ccaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aacta
//
LOCUS       DARBFD010000034        23976 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000034 DARBFD010000000
VERSION     DARBFD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.034"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            476..1339
                     /locus_tag="SCK08_001324"
     CDS             476..1339
                     /locus_tag="SCK08_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1201208.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="SCK08_001325"
     CDS             complement(1468..3090)
                     /locus_tag="SCK08_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1201209.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="SCK08_001326"
     CDS             3339..3860
                     /locus_tag="SCK08_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1201210.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCK08_001327"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCK08_001327"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEG1201211.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="SCK08_001328"
     CDS             5941..6759
                     /locus_tag="SCK08_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEG1201212.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="SCK08_001329"
     CDS             complement(6862..7059)
                     /locus_tag="SCK08_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201213.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="SCK08_001330"
     CDS             7228..7806
                     /locus_tag="SCK08_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1201214.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="SCK08_001331"
     CDS             7901..8038
                     /locus_tag="SCK08_001331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201215.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="SCK08_001332"
     CDS             complement(8851..9354)
                     /locus_tag="SCK08_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1201216.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="SCK08_001333"
     CDS             9713..10738
                     /locus_tag="SCK08_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1201217.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="SCK08_001334"
     CDS             11061..11939
                     /locus_tag="SCK08_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEG1201218.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="SCK08_001335"
     CDS             12415..12531
                     /locus_tag="SCK08_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1201219.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="SCK08_001336"
     CDS             13459..13797
                     /locus_tag="SCK08_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201220.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="SCK08_001337"
     CDS             13891..14121
                     /locus_tag="SCK08_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201221.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="SCK08_001338"
     CDS             14521..14892
                     /locus_tag="SCK08_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1201222.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="SCK08_001339"
     CDS             complement(15166..15858)
                     /locus_tag="SCK08_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1201223.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="SCK08_001340"
     CDS             complement(15861..16646)
                     /locus_tag="SCK08_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1201224.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="SCK08_001341"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="SCK08_001341"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG1201225.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="SCK08_001342"
     CDS             18308..18625
                     /locus_tag="SCK08_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1201226.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="SCK08_001343"
     CDS             18638..20311
                     /locus_tag="SCK08_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEG1201227.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="SCK08_001344"
     CDS             20325..21956
                     /locus_tag="SCK08_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEG1201228.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22828..>23976
                     /locus_tag="SCK08_001345"
     CDS             22828..>23976
                     /locus_tag="SCK08_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HEG1201229.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAE"
BASE COUNT         7479 a         4078 c         4844 g         7575 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgcgatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactctg ttttcaacgt aaagtgtata aaacagaatg cgatactatc
    22501 aagggagtta aaacaatata cagaacagta aaacactata aataaaaata aaagtgcaat
    22561 aaaagtgcaa tttttgcacc taatcatact ctgtttatat atgaaaacta gattctgaat
    22621 ccaatatttg gttaacgaga ggcgttcgaa tttgaacgtc ttttatatat tactttcaaa
    22681 taaatatgag gtagaatact atctattttc tttttaaccc aattgttata atggagagtg
    22741 taaaatattt tgtgtaaata gaaaaaagga agtcccttct gtagaataga gttaccacaa
    22801 cacattcaca gaagagagga cttccctatg aacgatttta ctacagaaat tctaaagact
    22861 ctagcgaaca aaggcgattt gaatgaatta ttccgtgtcc atttggagaa agcagtcaat
    22921 acgcttctca aaacggagtt aacggctttc ctagattacg aaaagtatga tcgcattggt
    22981 tttaacacgg gtaattctcg taacggctcc tatgaccgta cggtcaagac cgagtatggg
    23041 gaacttcatc tccagattcc gcgcgaccgc aatggtgagt tcaagcaaca gactgttcct
    23101 gcttatagac ggacgaatga cacgttagag gagaccgtca ttcacctctt ccgaaaaggt
    23161 attaccatgt cggaaatcgc agacttgatt gagaaaatgt atgggcatta ctacacgccc
    23221 caaaccatgt ccaatataac aaaatcattt acagaagagg taacggcgtt taatgggcgg
    23281 gagcttcatg accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg
    23341 aaaaccgtcg ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag
    23401 gaagtattga gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta
    23461 ttggaccttc aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag
    23521 gggatggtag gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta
    23581 cacgtttccc gtaatatcag tcataaagtg cgtgtcgatg atcgtaagga agtctgtgat
    23641 gattttaaaa tggtgtatca agcctcatct aaagaggtgg cgttggaagc acgtggtact
    23701 tttgcggaaa aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat
    23761 cacttgctca ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac
    23821 ttgattgaat cctttaataa acaaatcaag aaatacagcc accgcaagga acagttccaa
    23881 aatgaagagt cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc
    23941 ctaggtcgca gtcataaagg tttccaacag gccgaa
//
LOCUS       DARBFD010000035        23831 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000035 DARBFD010000000
VERSION     DARBFD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.035"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(55..789)
                     /locus_tag="SCK08_001346"
     CDS             complement(55..789)
                     /locus_tag="SCK08_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1201230.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="SCK08_001347"
     CDS             1116..1526
                     /locus_tag="SCK08_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEG1201231.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="SCK08_001348"
     CDS             1519..2220
                     /locus_tag="SCK08_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEG1201232.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="SCK08_001349"
     CDS             2220..3806
                     /locus_tag="SCK08_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201233.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="SCK08_001350"
     CDS             3825..4046
                     /locus_tag="SCK08_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEG1201234.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="SCK08_001351"
     CDS             4043..4804
                     /locus_tag="SCK08_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEG1201235.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="SCK08_001352"
     CDS             5184..5693
                     /locus_tag="SCK08_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1201236.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="SCK08_001353"
     CDS             complement(5768..6307)
                     /locus_tag="SCK08_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEG1201237.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="SCK08_001354"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="SCK08_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEG1201238.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="SCK08_001355"
     CDS             7320..8171
                     /locus_tag="SCK08_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEG1201239.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="SCK08_001356"
     CDS             8199..8834
                     /locus_tag="SCK08_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEG1201240.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="SCK08_001357"
     CDS             8854..9627
                     /locus_tag="SCK08_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1201241.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="SCK08_001358"
     CDS             10035..10832
                     /locus_tag="SCK08_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEG1201242.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="SCK08_001359"
     CDS             10810..11223
                     /locus_tag="SCK08_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1201243.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="SCK08_001360"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="SCK08_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1201244.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="SCK08_001361"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="SCK08_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEG1201245.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="SCK08_001362"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="SCK08_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEG1201246.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="SCK08_001363"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="SCK08_001363"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEG1201247.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="SCK08_001364"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="SCK08_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEG1201248.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="SCK08_001365"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="SCK08_001365"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEG1201249.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="SCK08_001366"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="SCK08_001366"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEG1201250.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="SCK08_001367"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="SCK08_001367"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEG1201251.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="SCK08_001368"
     CDS             21907..22485
                     /locus_tag="SCK08_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1201252.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="SCK08_001369"
     CDS             22537..23019
                     /locus_tag="SCK08_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEG1201253.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="SCK08_001370"
     CDS             23136..23510
                     /locus_tag="SCK08_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201254.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DARBFD010000036        23626 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000036 DARBFD010000000
VERSION     DARBFD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.036"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            31..1692
                     /locus_tag="SCK08_001371"
     CDS             31..1692
                     /locus_tag="SCK08_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1201255.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1762..2109)
                     /locus_tag="SCK08_001372"
     CDS             complement(1762..2109)
                     /locus_tag="SCK08_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEG1201256.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2193..3134
                     /locus_tag="SCK08_001373"
     CDS             2193..3134
                     /locus_tag="SCK08_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201257.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3135..4178
                     /locus_tag="SCK08_001374"
     CDS             3135..4178
                     /locus_tag="SCK08_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201258.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4184..5254
                     /locus_tag="SCK08_001375"
     CDS             4184..5254
                     /locus_tag="SCK08_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201259.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5244..6197
                     /locus_tag="SCK08_001376"
     CDS             5244..6197
                     /locus_tag="SCK08_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1201260.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6209..7219
                     /locus_tag="SCK08_001377"
     CDS             6209..7219
                     /locus_tag="SCK08_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1201261.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7504..8325)
                     /locus_tag="SCK08_001378"
     CDS             complement(7504..8325)
                     /locus_tag="SCK08_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1201262.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8422..8700)
                     /locus_tag="SCK08_001379"
     CDS             complement(8422..8700)
                     /locus_tag="SCK08_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201263.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8968..10977
                     /gene="metG"
                     /locus_tag="SCK08_001380"
     CDS             8968..10977
                     /gene="metG"
                     /locus_tag="SCK08_001380"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEG1201264.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11456..12043)
                     /locus_tag="SCK08_001381"
     CDS             complement(11456..12043)
                     /locus_tag="SCK08_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201265.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12218..13540
                     /locus_tag="SCK08_001382"
     CDS             12218..13540
                     /locus_tag="SCK08_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1201266.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13695..14471
                     /locus_tag="SCK08_001383"
     CDS             13695..14471
                     /locus_tag="SCK08_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1201267.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14464..15033
                     /gene="rnmV"
                     /locus_tag="SCK08_001384"
     CDS             14464..15033
                     /gene="rnmV"
                     /locus_tag="SCK08_001384"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEG1201268.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15026..15910
                     /gene="rsmA"
                     /locus_tag="SCK08_001385"
     CDS             15026..15910
                     /gene="rsmA"
                     /locus_tag="SCK08_001385"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEG1201269.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16361..16990
                     /locus_tag="SCK08_001386"
     CDS             16361..16990
                     /locus_tag="SCK08_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201270.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17114..18223)
                     /gene="ugpC"
                     /locus_tag="SCK08_001387"
     CDS             complement(17114..18223)
                     /gene="ugpC"
                     /locus_tag="SCK08_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEG1201271.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18432..18854
                     /gene="mgsA"
                     /locus_tag="SCK08_001388"
     CDS             18432..18854
                     /gene="mgsA"
                     /locus_tag="SCK08_001388"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEG1201272.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19177..19713
                     /locus_tag="SCK08_001389"
     CDS             19177..19713
                     /locus_tag="SCK08_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1201273.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19753..21516)
                     /locus_tag="SCK08_001390"
     CDS             complement(19753..21516)
                     /locus_tag="SCK08_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201274.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21519..23234)
                     /locus_tag="SCK08_001391"
     CDS             complement(21519..23234)
                     /locus_tag="SCK08_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201275.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7613 a         4313 c         4802 g         6898 t
ORIGIN      
        1 tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga aatttacatt tggtttggtt
       61 gcggtatgcg gccttgtact tgctggatgt tatggcggca gttcagccac aagcagtagt
      121 tcagcaaaaa gcggttcagc agatggagga ggcgtattta acttagtcgt gcctcaagaa
      181 atgcctacag ctgacttatc agttgcaaca gatacaatca gttttacagc attgaataac
      241 gtttatgaag gaatttaccg tcttgatgaa gacagcaaac cacagccggc aggagctagc
      301 gagttggcag aagtcagtga agacggatta acttacaaaa tcaaattacg agaagacgca
      361 aaatggtcaa atggtgatcc tgttacagca gcggattacg tttatggctg gcaacgaaca
      421 gtagacccag ctacagcttc tgaatatgct tacctgtatg cgccagttga aaatgcagaa
      481 gacattacag ctggaaagaa agataaatca gaactaggga tcaaagcagt tggtgactat
      541 gaattagaaa tcaaattgac gaaacaaaca ccatatttcc aatatctgct tgctttccca
      601 tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg gggatgccta tgcatctgca
      661 agtgataaag ctgtttataa tggaccattc acattagaag attttgatgg accaggaaca
      721 gatacagaat ggacttataa gaaaaatgat cattactggg acaaagatac agtaaaactt
      781 tctgaaatca aagtcagtgt agtaaaagaa tcttctactg cattgaactt attcaaagat
      841 ggccaagcag atgacgtgat cttgtctggt gagttagcac agcaaaacgc aaacgatcca
      901 gcttatactt ctgttaaaga agcgcggaca agctatatcg aattcaatca acgtgaaaag
      961 aattcaccat tcaacaatgt gaacctaaga aaagctattt cttattctat cgatcgtgaa
     1021 gctttagtaa aacaagtact aggtgacgga tcagttgtct ctacaggatt gatcccatca
     1081 gatatggtaa aaagtccaac aacgaacgaa gactttgcaa aagaagctgg aaaattagtt
     1141 tcttatgaca aagataaagc aaaagaatat tgggaaaaag cgaaaaaaga attgggcgtt
     1201 gattctttag aatttgattt gatggcttct gatgatgatt catcgaaaaa agtaatcgaa
     1261 tatgttcaaa actcaatcca agaaaacttg gatggcgtaa cagtcaaacc aacacctgtt
     1321 ccattctctg tacgtcttga ccgctcaact tcgggtgatt ttgacgcagt actaggcgga
     1381 tgggctgctg actatgcaga cccaagcagc tttactgatt tgtttgtcac aggtaattca
     1441 tacaaccgtg gtcgctggag caatgcagaa tatgacgaag cagttaaagc agcttcatca
     1501 acagacgctg gcgatgaaca agcccgttgg gatgatttcc aaaaagcaat caagatcatc
     1561 tctgatgata tgggtgtcgt tccggtgtac caaaaagcag aaggtcacct agtgaatgaa
     1621 aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat gggattacaa atggacatac
     1681 gttgaagaat aatcgttaat accgtgagca tgagaaaaaa cgaggctgcg gcaaatgtcg
     1741 caacctcgtt ctcatattca cttatcctaa caaagaaaaa agcatgaaaa tagcagaaag
     1801 agcaattagt attccggcaa tgatcagcca aaagctgtac ataccgtagc tggcagacga
     1861 gagcgaggaa aattctggtt ttttctttcg gttccttgtt cgtgcaagat ggaccgatcg
     1921 ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa aaaccgccga taatcagaaa
     1981 cggcaatgac cataagaata gatcattaga taattgaatg agtgtaaagg aatggttgat
     2041 gagatttttt attaaaataa ccagaatcag tagaaaagct actgcgtagg ggatagattt
     2101 ttttttcatt tgattgctcc tttatttttg gttcattatc taataatgta gcgaaacatc
     2161 tgtcagaagt caaaagctag ggggaaacat tagtgaatag ttatatcaaa tatgtattaa
     2221 aacgtgtctt tttcatgatc atcacgcttt ggttaattgc aacaatcacc tttttcttga
     2281 tgcaattgct gccgggcacg ccatatacga atcaggaacg tttgagtcct gaaacaatcg
     2341 agatgctaaa taaacaagtc ggtttggata aaccagttat cgtacaatat gggatttatc
     2401 tttctaattt gcttcaagga gattttggaa tttccttcca atttaaaaac cagccagtcg
     2461 cacatttgtt agctggaaga atcggaccat ccttgcagtt agggttacag gcaattatct
     2521 ttggaacagt cctagggacg attttaggaa cgatctctgc catgaagcaa aatacatggg
     2581 cagatacatc ttctacactc gtagcgatat taggtcgttc cattccaaac tttgtatttg
     2641 ctgttttatt acaatatatc tttgcaatca aattacaagt tttgccaatc gcaaaatggg
     2701 atggctttgt gtacacaatc ttaccaacaa ttgcgttggc catgtcgccg cttgctgact
     2761 ctgctcggtt tatccgtacg gaaatggtgg aagtactgca tagtgattat gtggaattag
     2821 caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt
     2881 tgattccatt gatgacttta ttaggaccgt tagctgtagc attgatgacc ggatcactgg
     2941 tggtagaaaa tatctttgct attcctggta tcggggaaca attcgttaag tcaatcacaa
     3001 caaatgatta tcctacgatc atggctgtaa cgatccttta ctcatttatg ctgatttttg
     3061 ttattttgat cgtggatcta ttgtacggtc tagttgatcc aagaattcgt gtttcagaag
     3121 ggagccgagg ataaatggaa atgataccaa agcgttatga aacagttgca gatatccctg
     3181 cggatgaatt tcttccatta caaaaaaata ccgaagaaga acgtgaacag attactgcac
     3241 cttctttgaa ttttatccaa gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta
     3301 tttcaatggt tttactattg gtcatcattt tgatctctgt gatcacaatc tttgtttctc
     3361 ctcatgatcc aactgcccaa aacgtggctt atatcaattt gcctcctcgt attccgggaa
     3421 tcaatatcga tggattgaat ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg
     3481 ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg aacagacggt ttgggacgtg
     3541 atgtattaag ccgcttattt atgggaatga gaatctcgtt gctgatcgca tttattgctg
     3601 cattactaga tgtaacgatt ggtgtagctt acgggttggt ttctggtttg ttaggcggac
     3661 gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc aggaattcca aacctcgttg
     3721 tcatgatttt gatgttggtc gtttttgaac caggaatttt ttcaatcgtg gctgctatgg
     3781 ccatcacaaa ctggatacca atggcacgta tcgttcgtgc acaaacaatg aaattaaaag
     3841 atcaagaata tgttttagct ggattgacat taggtgaatc aagaatgaaa attgcattca
     3901 aacatatttt gccgaatatc tctagtgtca ttattattca aatgatgttt agtattccat
     3961 ctgctatttt cttcgaagcc tttttaagct ttatcggttt aggtcttacg cctccgtcag
     4021 cttcattagg aacgatgttg agcgaagggt acaagacatt cctgtatctg ccttatctat
     4081 tatggattcc ggcagtaact ttatcggtta tcatgattgg ctttaactta cttgctgatg
     4141 gattgcgtga tgcctttgat cctaaaatga aagagtgaag acgatgacga aaattttaga
     4201 agtgaaagac ttagaaatct cttttgatac gtatgctgga aaggtacgag cgattcgcgg
     4261 tgtcaatttt catttgaata aaggagaaac attggcgatc gttggggaat ctggaagtgg
     4321 aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca agcaacgcca atatagatgc
     4381 tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa
     4441 aatccgtggt aaagaaatag cgatgatttt ccaagatcca atgacctctt tagatccgac
     4501 gatgcccatt gggaagcaag tcgctgagtc ccttagaaag cataataaag tttcaaaaaa
     4561 agagggacaa aaagctgcat tagaattgct taaattagtt ggaatacccg aagctgagaa
     4621 acgtatcaac agttatccgc atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc
     4681 gattgcactg gtatgttatc cggaaatcct gattgctgat gaacctacaa cagcgcttga
     4741 tgtgactatt caagcacaga ttttggagct gctgaaagaa attcagacaa aaatcgatac
     4801 atcaattatc tttatcactc atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc
     4861 tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg gaagaaattt tctacaaccc
     4921 gcaacatccc tatacttggg gactgcttgg ttcaatgccg acattagata gtgcgaacga
     4981 tcgattgtat gctattcctg gttctccacc ggatttattg aacccgccaa aaggagacgc
     5041 attctatcca agaaatgaat tcgcgatgaa aatcgatgca gaacaagaac caccattttt
     5101 tgaactatca aaaacacaca aggcagcaac ttggctactt gctccgcagg cacccaaagt
     5161 gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt aaagagaaac aaaaacatac
     5221 ttacggtcaa ggaggaattg ctgatgccaa ataaactact tgaagttaaa ggattgaaac
     5281 agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt aaatgacatc agctttcaca
     5341 tttatgaagg agaaacattc ggtttagttg gagaatctgg aagcggaaaa tccactactg
     5401 gccgtacgat cattcgcttg aatgaaccta ctgatggtga gatattattc gacggtcaag
     5461 atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga
     5521 tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa agtcagagat atcattgccg
     5581 aaggaattga tgttaatgga ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg
     5641 atctattgag aactgttgga ttgaatccaa gccacggtac tcgttatcct catgaatttt
     5701 caggtgggca acgccagcgt attggtattg cacgagcatt ggcagtaaaa ccgcgtttta
     5761 tcatttgtga tgaaccaatt tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt
     5821 tgttgcaaga tctccaaaaa gaacatcaat tgacctatct atttattgca catgatttgt
     5881 cgatggttaa acatattagt gaccgaatcg gtgtaatgca taacggcttg ttattagaga
     5941 tgggaacaag tgatgaaatc tataatcacg gtgttcatcc ttatacagaa agtttgttat
     6001 ctgctattcc tctaccagat ccagaccatg agagaaaacg cagaagaatc aaatatcaac
     6061 ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct
     6121 atgcaactga gcaggaagtc ccatattacg caaaaaaact aaaacgacaa aaggaatctc
     6181 ttctggttgc taactaggag ggacatagat gctaaaagct tttaaattcc gtatgtatcc
     6241 gacagaagaa caaaaacagc aattgattcg tacttttgga tgtgctcgat ttacctataa
     6301 ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca ggagtagcca atttttcgct
     6361 cacacccgct acattaaaaa aagaataccc attcttaaaa gaagtagaca gtcttgcttt
     6421 agcaaatgca cagcttaatt tggatcgggc ttttcgcaat tatttcaagg gacgtgccag
     6481 cttcccaaag cttaaaacga aaaaaagtat gtggcaatct tatacgacca acaaccaaac
     6541 ccgtacgatc tatctggaaa atggctactt aaagttacca aaacaaaaag aactaatcaa
     6601 gataaatcag catcggccag ttgaaggatc gattcgctcc gctacgattt ctgctcgata
     6661 taatgaagaa ttttatgtcg cactactttg tgatgtttcg ccagtaaaaa aagaatcttt
     6721 agctaaatgg atcgggatcg cctatcatcc gaagacattg atccaaacat ctcgacctct
     6781 agaagtaact ttaccaaaat ttcaccagac agaagaaaag ctgcaacatg cacagcggaa
     6841 attgaatgtg aaagtccgct ccgctcacca tagaaagatc aggttagatc aggcaagcaa
     6901 ttatcagaaa caaaagagaa aagtaatgga tctttatctg aaacaaaaga accagcgaga
     6961 agattatctt gaacaattat ctgggaaatt ggtcaaacaa tatgactact tatttgtaga
     7021 atcttttcca aaagaagagg cacatgctga tttttctatt cacgactggc ataaattaat
     7081 aacaaaattg cgttataagt cccaatggta taataaaaaa tttcttttga tcaataccga
     7141 cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca
     7201 ttcggttatc aagggataaa aaatgaatga aactgatagg agcgttaggg ttagccttgt
     7261 taataaaaag tcttaatgac ttttgtttct aggaattttt cttttgaaga aaagctcact
     7321 aattatgaca aaccaataaa acaatagcag aaaaagttcg tacgagcttt ttctgctatt
     7381 gttaattttt tattcagaaa gtttacgaat acatcaaaat tctgaaacaa aaaacgtaac
     7441 tcacaaagag ttgtttttgc gttttttctt gaatgattag gtctgaatct tttgctcaca
     7501 accttattca tggtgccccg gcttgagctg atagattttt gaaggacggc cgattcctac
     7561 aggacgttca ccgatctcat cgaaatattg gagcatagct ttcttaaaat tggaatgatc
     7621 gatcgtttta taatcgatcc ctaagaattt agcaaatact tttcgagctt ccgtaatcgt
     7681 gaaatcttta cctaaaactt gtagaacttg cggctcgtgt tccattttat tgactacacg
     7741 gttgaatgct ttcaagatga tttgactatg gtcaaaagcc aaagtatctt tgccagaaga
     7801 ctctccagtg actaaatcaa gcgagatagc cacttctccg ctagataaat ggatcttgtt
     7861 actatgacgt tctagtgtga accagcgtac ttccttggca tcatctccgg cgattagcgg
     7921 ttcttctcca ataaaggcta agtagctgac tgtgacgacc cagcctcgcg gatctcgatt
     7981 aggcgtactg aatgtatgca gttgctcaat attttctttt gaaataacca caccagtttc
     8041 ttcctttgtt tctcttaaga cgctttctcc agttgactca tccttttgga caaagccacc
     8101 aggcaatgcc catgaatttc gataaggatg cccttttctt tggatcaaca aaactttcag
     8161 ctgatcttct tctttgttat agcacatcag tacgatatcg acagtcaaag agggcttgtc
     8221 atattctgga cgttcctgtt tttgatacca gttaagaaat tcttcttcac ttgctttatg
     8281 ttcataatat aatttttctt cttctttaga agcaaaacgg ttcatctgac cacctccatt
     8341 gacagtaaaa gtacttttta ccttattgta gcagactctg aaaaaaaacc aaggatttac
     8401 ttgaaaaatc taggttatca tttaaatatg aaaaataaat ggaagtatga gattgaataa
     8461 aaagaaaagc agataaaacg gcaatacttt ataccgctct tcacgaatgg caaagaagcc
     8521 acaaataacg atatatacgt caatgataaa acgcaaaatc caagctgctg tcataaaact
     8581 gttcaaatgc ccatggaggt taaaataacc atacatacga atataaaaat agctgaatat
     8641 taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa aaaagatgtt tcttctgcat
     8701 gattgttcca gtcccttact aagatagata gtctttatta taccacgtgt gaggggagaa
     8761 ataaaaagcc gttttttagt agatatgaaa caaaacaaaa acccgcattt attcaaccta
     8821 ttcatccttt taaaggatag attgaacgta gatagaagtc gtttatccag tctttttcat
     8881 tttgaaaaaa agctaacagc atgctttttt tatggtatac tagcaaggaa acgaattcgc
     8941 atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg aaacttttta tatcactacg
     9001 ccaatctatt atccaagcgg caaacttcat atcggcaact cgtatactac gattgcatgt
     9061 gacgcaattg ctcgttataa acggttgatg ggctttgatg tgttttatct cactggagtg
     9121 gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac taggtgtaaa accgcaagaa
     9181 tatgtagata aaatggcggc tgatgtcaag aaattatgga aaacgcttga tatcagttat
     9241 gataaattca ttcgcacgac agatgattac cataaagcag ctgttcaaaa aatatttgat
     9301 cgtttattag aacagggaga tatttactta ggtgaatatg aaggctggta ctctgtttct
     9361 gacgaagaat tctttacaga aacacaactt gcagaagtgt acaaagatga cgaaggtaac
     9421 gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat tggtaaaaga agaatcatac
     9481 ttcttccgca tgagcaaata cgcagaccgt ctattggaat actatgaaga acatcccgaa
     9541 ttcatccagc cagaatctcg taaaaacgaa atgatcaaca actttatcaa accaggatta
     9601 gaggatcttg cagtttcacg aacaactttt acttggggag taccagtcaa gagtgatccg
     9661 aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt atattactgc actaggatat
     9721 ggctccgaag atgaaagtct attcgaaaaa tactggcctg cagatgttca gatggtcggt
     9781 aaagaaatcg ttcgctttca tacgatctat tggccaatca tgctgatggc ccttgatttg
     9841 cctctaccta aaaaagtatt tgggcatggc tggctattga tgaaagacgg aaaaatgtct
     9901 aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg aacgttatgg tttagatgca
     9961 ctccgttatt atttgcttcg tgctgttccg tttgggtcag atggtgtctt tacgccagaa
    10021 gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc tagggaactt attgaatcga
    10081 acgattgcta tgatcaacaa atattgtgat ggactcgttc ctgactatgc ctcacttgtc
    10141 actccatttg atagtgaact gtctacgacg gctgctaacg ttgtcggacg ttaccatgat
    10201 gcaatggaaa aaatggaatt caatacagca ttggctgaaa tctgggtgct gatttctcgt
    10261 gcaaacaaat acattgatga aacagaacca tgggtattag caaaagacga agaaaagaaa
    10321 gctgaattga atagtgtcat gatccattta gctgaatctt tgcgtatcgc agcgatatta
    10381 cttcagccaa tcatgacaga aacgccagag aagatcttca atcaactagg attggactct
    10441 gaaacaatga atcttgaagg attacacttt ggcgaatttc cttcaggaac aaaagtagtt
    10501 gcaaaaggta caccaatctt cccacgttta gatatggaag aagaagttgc ttttattcaa
    10561 gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag tcaaatggga tccagaagaa
    10621 acagaacttg tttcaacgaa agaaaaacaa atcaaatttg atgtatttga aaaagtagaa
    10681 ctaaaagtag cagaagtcat caactgtcaa aaagtagaag gggcagataa gttgctgcaa
    10741 ttccgactag atgctggaga cagccaagac cgtcaaatct tgtcaggaat cgctgaattt
    10801 tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca
    10861 cgaaagatgc gtggacaaat cagtcaagga atgatccttt ctgcagaagc accagatggc
    10921 tctttacaag taatcgaagc accaaaatcc atgccaaatg gctcagaaat cgcatagaaa
    10981 ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt tgagaagcaa
    11041 ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa cagagaacta
    11101 tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta atttagaata
    11161 atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc gggcagattt
    11221 taattgattc atctggtact cttttttatt gtccttacat tcataaagtt tgaatgcaat
    11281 gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat tataagttct
    11341 atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa
    11401 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
    11461 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
    11521 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
    11581 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
    11641 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
    11701 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
    11761 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
    11821 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
    11881 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
    11941 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
    12001 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
    12061 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
    12121 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
    12181 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
    12241 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
    12301 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
    12361 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
    12421 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
    12481 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
    12541 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
    12601 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
    12661 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
    12721 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
    12781 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
    12841 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
    12901 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
    12961 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
    13021 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
    13081 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
    13141 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
    13201 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
    13261 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
    13321 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
    13381 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
    13441 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
    13501 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
    13561 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
    13621 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
    13681 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
    13741 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
    13801 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
    13861 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
    13921 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
    13981 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
    14041 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
    14101 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
    14161 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
    14221 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
    14281 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
    14341 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
    14401 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
    14461 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
    14521 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
    14581 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
    14641 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
    14701 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
    14761 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
    14821 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
    14881 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
    14941 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
    15001 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
    15061 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
    15121 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
    15181 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
    15241 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
    15301 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
    15361 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
    15421 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
    15481 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
    15541 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
    15601 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
    15661 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
    15721 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
    15781 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
    15841 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
    15901 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
    15961 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttgttaccgc
    16021 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
    16081 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
    16141 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
    16201 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
    16261 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
    16321 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
    16381 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
    16441 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
    16501 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
    16561 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
    16621 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
    16681 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
    16741 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
    16801 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
    16861 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
    16921 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
    16981 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
    17041 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
    17101 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
    17161 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
    17221 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
    17281 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
    17341 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
    17401 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
    17461 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
    17521 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
    17581 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
    17641 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
    17701 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
    17761 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
    17821 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
    17881 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
    17941 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
    18001 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
    18061 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
    18121 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
    18181 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
    18241 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
    18301 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
    18361 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
    18421 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
    18481 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
    18541 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
    18601 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
    18661 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
    18721 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
    18781 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
    18841 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
    18901 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
    18961 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
    19021 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
    19081 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
    19141 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
    19201 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
    19261 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
    19321 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
    19381 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
    19441 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
    19501 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
    19561 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
    19621 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
    19681 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
    19741 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
    19801 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
    19861 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
    19921 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
    19981 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
    20041 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
    20101 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
    20161 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
    20221 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
    20281 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
    20341 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
    20401 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
    20461 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
    20521 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
    20581 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
    20641 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
    20701 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
    20761 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
    20821 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
    20881 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
    20941 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
    21001 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
    21061 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
    21121 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
    21181 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
    21241 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
    21301 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
    21361 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
    21421 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
    21481 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
    21541 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
    21601 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
    21661 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
    21721 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
    21781 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
    21841 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
    21901 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
    21961 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
    22021 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
    22081 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
    22141 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
    22201 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
    22261 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
    22321 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
    22381 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
    22441 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
    22501 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
    22561 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
    22621 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
    22681 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
    22741 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
    22801 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
    22861 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
    22921 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
    22981 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
    23041 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
    23101 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
    23161 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
    23221 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
    23281 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
    23341 aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaagaaa
    23401 atgagaaaac atgccagtct cttcattcat aggaacgtct attttactta atttttctat
    23461 aaatgaattc tgcataaaaa atatatcaag taaatgttta tagatgaaac tctatttact
    23521 tcttttttaa tttgttcttg caatcttttt tgctacgtaa tttttgtaaa aaagtgggtt
    23581 gagagaaatc taattgattt tcaaagaagg agtatgaaaa tgagaa
//
LOCUS       DARBFD010000037        23508 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000037 DARBFD010000000
VERSION     DARBFD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.037"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            37..354
                     /locus_tag="SCK08_001392"
     CDS             37..354
                     /locus_tag="SCK08_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1201276.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            347..955
                     /locus_tag="SCK08_001393"
     CDS             347..955
                     /locus_tag="SCK08_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEG1201277.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            1010..1801
                     /locus_tag="SCK08_001394"
     CDS             1010..1801
                     /locus_tag="SCK08_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEG1201278.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            1939..2451
                     /locus_tag="SCK08_001395"
     CDS             1939..2451
                     /locus_tag="SCK08_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEG1201279.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            2477..3349
                     /locus_tag="SCK08_001396"
     CDS             2477..3349
                     /locus_tag="SCK08_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1201280.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            3525..3887
                     /locus_tag="SCK08_001397"
     CDS             3525..3887
                     /locus_tag="SCK08_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1201281.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            3896..4648
                     /locus_tag="SCK08_001398"
     CDS             3896..4648
                     /locus_tag="SCK08_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201282.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            4662..6464
                     /locus_tag="SCK08_001399"
     CDS             4662..6464
                     /locus_tag="SCK08_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201283.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            6741..7781
                     /locus_tag="SCK08_001400"
     CDS             6741..7781
                     /locus_tag="SCK08_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1201284.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(7833..9977)
                     /locus_tag="SCK08_001401"
     CDS             complement(7833..9977)
                     /locus_tag="SCK08_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1201285.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(10334..11707)
                     /locus_tag="SCK08_001402"
     CDS             complement(10334..11707)
                     /locus_tag="SCK08_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1201286.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(11707..12102)
                     /gene="rbsD"
                     /locus_tag="SCK08_001403"
     CDS             complement(11707..12102)
                     /gene="rbsD"
                     /locus_tag="SCK08_001403"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEG1201287.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(12130..13047)
                     /gene="rbsK"
                     /locus_tag="SCK08_001404"
     CDS             complement(12130..13047)
                     /gene="rbsK"
                     /locus_tag="SCK08_001404"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEG1201288.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(13155..14168)
                     /locus_tag="SCK08_001405"
     CDS             complement(13155..14168)
                     /locus_tag="SCK08_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1201289.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(14249..14848)
                     /locus_tag="SCK08_001406"
     CDS             complement(14249..14848)
                     /locus_tag="SCK08_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1201290.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            15034..15735
                     /locus_tag="SCK08_001407"
     CDS             15034..15735
                     /locus_tag="SCK08_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201291.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            15745..18456
                     /locus_tag="SCK08_001408"
     CDS             15745..18456
                     /locus_tag="SCK08_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201292.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            18853..20481
                     /gene="treC"
                     /locus_tag="SCK08_001409"
     CDS             18853..20481
                     /gene="treC"
                     /locus_tag="SCK08_001409"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEG1201293.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(20541..21722)
                     /locus_tag="SCK08_001410"
     CDS             complement(20541..21722)
                     /locus_tag="SCK08_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1201294.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            21974..23497
                     /locus_tag="SCK08_001411"
     CDS             21974..23497
                     /locus_tag="SCK08_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1201295.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT         7656 a         4202 c         4798 g         6852 t
ORIGIN      
        1 aataaaatat tacacttgag aatagaaggt gacgtaatgc acatccagat tccaactgtt
       61 cttcttgatg gtactgtgct agcagcatta agcaaggaag atatgtatgg gtatgcctta
      121 acaaaaatcg tacaagaaag tctctctgtc agtgaatcta cactatatcc ggttttgaga
      181 agattgaaaa aagaaggata tcttgagacc tatgatgaac cttttgaagg cagaaaccgt
      241 cgatattact cgttgacacc aatgggaagt cgtcatttag aagaaattat tgatgaatgg
      301 attgtttttc gagattcttt agaccggtta ttggaggaga gcacaaatga atgaatattt
      361 gaatgaagtt gtggatcatc ttcaagaaat tcctgaagac gatcgttttg atttgatcca
      421 gtattatgag gaatattttt tagattctgg aaaaacatta gatcagatca ttgatgaata
      481 tggtacacca aagcagtttg ccttaaaatt aaaaatcagt tacttttcag aagaagaaca
      541 agcagaaaac gagttagtga aaaatccaaa aagacaaatt cgcctgattt ggttaatcat
      601 tatcggttta tttgcttctc ctgtcctcat tccactagca ctggggttta tattggttat
      661 tgggggacta ttggttgctt tactagcgct catttttagt atctatgttt tatgtatcag
      721 tatcttaggt gttggcctat tcatgatcat ctcaggaatc agtgtgtttg gacaatcgat
      781 tctgagtgga ttattcttta tcggtgtcgg tctgttcgcc acaggaagcg cagtatttat
      841 ttcgccactg ttgctgaaag gaacaaaagg gcttttttat gtcatgatga actttgttaa
      901 atggatggga cgacgtttta ttacaaaaag agatctgcat tcagcgggcg tttgaaggag
      961 gacacataaa ttgagattga aacatttttt gattaccggt attggtctga tgcttactgg
     1021 tggcctccta gcaggtgcaa cttatacatt aggcgctcaa aagagtctga cttgggataa
     1081 tggtcctcgg ttgatcgata tggttactga gacaaaaaaa atcaaggatg caaacaaaat
     1141 cgtcatccat gcagaaaatc aaacagtttc ggtcatacgc ggattggatt ttgagataaa
     1201 aacgacttat gataagacag aaaagccaga aataaaggag aaaaatggcg tgttgactgt
     1261 gaatgcagtc aaaaaatcaa caggtgcaat cattagttta gaagacacaa gtgcacaaat
     1321 cactattgtt gtccctgcag atcatgaatt atctgaattg acaatcgaag ggaataatag
     1381 tgcaattgat atcaacgaag tatcaatcaa aaaagtgaat actaagacac atgatggctg
     1441 ggtatcgttc aatgaattta caggaggaac aataactgcg aaagatgaat taggctcact
     1501 tcgattagat gaggccaatg tggataactt gatcgtcaca ggtgaaaaca gcaacttcca
     1561 tctagaagac agtttgatca gcaaagaagg aaaagtcatc ctaaacggtg gcaatttatc
     1621 tttgcatgag atggaaaaca gtggctatac tatccaaacg tctggtgaat cttatattga
     1681 aaaagactac cagccaataa caaatacttt gttagaacaa ggacagaaaa agctaaagat
     1741 cactgctaaa gacacatcga tcaatattac gacggatcaa gaggacaatg aaacagattg
     1801 atcctgtact caactggata ttttaagaaa aaacagcaaa gatccttcca atctttgctg
     1861 tttttcatcg cttaacgcac ataactatag taaaataaga aagaaaatat ctaaaaagaa
     1921 aacagggtga aagagtatat gaaggtagaa gaaggtcaaa gagaaaagtt aaaaacagaa
     1981 atggaaagat tacatactta tatcactcaa ctaagccaaa ccttctatga tccagacaaa
     2041 gaaaaagtga tggttaatta cccgaacaat tcagaaggtc gccaattaga gcaagtgtac
     2101 catgaagttt tcaaacattt attgacggtg aagaaagagc tggattatta ttctttaccg
     2161 attatcgata caggaatatt gaagtatgat gggaagaagg aacgatttat ctttaaatct
     2221 gttcgggaag atttgcctct ttcagcaggg atggatctag aggtcttggt agaagactat
     2281 tttacagaag aaaagcactg ggttcgaaca aaattggatt atttaccgca ggcagccgga
     2341 gggacacagg caaggggttg gtacatcact gaagacaaag agttagaact agaaggtgta
     2401 atggcgcgta ttcgaaaaaa aattaaaaaa gtaacattag atatttattg aaggataaag
     2461 gagcgcagga agtagcatga gagaaaaaag agcattttat atcaatggtt atgcagggat
     2521 tgtcggattg gtcgtattag ctttagttgg attgtttttc ttctatttag gtatgtggca
     2581 agcgaaagtt tgggcacttt ttttaagtat ttttctttgg ttgatcacgt tattgttgtt
     2641 aagttcggca actgtagtca gtcctaatca agcaaaagta attttgtttt ttggacaata
     2701 tttaggtacg attcgagaaa atggcttttt ccttacgata cctttggcac aaaaaatgac
     2761 tgtttcgttg aaagttcgga atttcaatag ttctgtttta aaagtcaatg atttggatgg
     2821 aaaccccatc gaaatctcag cagtagtagt ctttaaagtc gttgatacag caaaggcatt
     2881 attcgatgta gcgtattatc aagattttgt agaaatccaa agtgagacag ccattcggca
     2941 tatcgctagc caatacccgt atgatacatt caatgagaat gatttgacgc ttcgaggaaa
     3001 cacgacggca gtgtctgacg agttgcaaaa agagctgcaa gagcgtttag cagtagcagg
     3061 tgtcgaagta atcgaaacac gcttgaacca tttagcctat gcaacagaaa tcgctagtgc
     3121 catgctccaa cgtcaacaag caaaagccat tttgtctgct cgacaaacca ttgttgaagg
     3181 agctgtcaca atcactcaaa tggccttaga gcaaatcgaa gatggacaag aaatcaattt
     3241 caccgatgat cggaaagtcc aattgatcaa taatttactt gtctcaatca ttacagacaa
     3301 aggaacgcaa cctgttatca atacaggcga catgaaagac cgtaagtgac atactttttg
     3361 taagatagga agaaggagtt taggacggat tattgtcttg aactcttttt ttagttgttt
     3421 taaagaaaat gacaaaattg aaagaagaat gatagttgat tcgaaggcag aattgttcgc
     3481 ttttttctat aatcagtata taaagtaaga gagaagggga aaaagtgaaa aaacttctga
     3541 tcggtctact tgttttcatc gttgtggtac ttgcaggggt gaaaatcgca gattacgtag
     3601 ttatgggtgg agaagaatac tatgtacaaa ttacgacaga tggtacaaaa acagtttcaa
     3661 aatcagactc tggacaagaa acagtcaact atagatatat cctgcctggc tatacgaaag
     3721 agggtaagga aaaagaactg gacttcaagg gacaattgac tcgaccgctt cgtaaaggag
     3781 catatttaaa aatcacctgg aacaaaaata aaggtgtcac ttcgtatgaa gaagtcgaaa
     3841 aaaaagacgt gccgaaaaca gcactggaaa aactaattaa agggtaggga aagaaatgaa
     3901 caaaatagtc gaagtacaga atgtaacaaa agtatacgga aaaaacaatg agaaaaagac
     3961 gcaagcatta agcggcatca gcttcgatgt agaaaaaggc gaatttattg ggatcatggg
     4021 cgcttctggt tctggtaagt cgacactttt gaatatttta tccacattag ataaaccaac
     4081 agatgggcat atccgaatca atcaaaaaga tgtgaccaca ttaaaaggaa atcaactagc
     4141 agattttcgt gcgaatgaga ttggctttat tttccaagat ttcaatttat tagaaagctt
     4201 gacagcacaa gaaaatatcg ctgttcctct ttctttacaa ggcgtcagac caaaagaaat
     4261 caagcaacgt gtccaaaaaa ttgcagaacg tttatccatc tctcatattt tggaaagcta
     4321 tccttctgag atttccggtg gacaaaaaca acgggtcgcc gccgcacgtg cattgatcac
     4381 acaaccaacg attctattag gggatgagcc tacaggtgcg ctggattcca aaagtgcccg
     4441 ggatctttta gatacgatgg acgaattgaa tacaaaagat catgtatcga ttcttcttgt
     4501 gacgcatgat ccgttttctg caagttattg ccaacgtatt ttattcatca aggatggcgg
     4561 tatccatcaa gaagtgaaac gtggagatca aagccgtgat tccttctatc gagaaatact
     4621 atctattctt ggaaacttgg aacagtaggg ggagattgac tatgctttgg aaattatcac
     4681 ttactgggat caagagccgt ttaagagact acttggtgtt attctcagga ttagttatgg
     4741 catctgctat tttttacatg tttgaatcaa tggcaagcaa cgaagctttt ttgaaaagta
     4801 atacgatcgt tgcggctaca gttatcattt tccgattcgg ttctgtttta ctagggatca
     4861 tcacattcgt ttatatttta tatgcaaatt cctttttgat gacgatgcgg caaaaagact
     4921 atgcaatgtt tatgatgctg ggagcaaaag gcagaaaaat cgcacagatg attttttctg
     4981 aaacattctt agtagggatc gcagctactt tagtgggttc agctgtcggt gtaggattga
     5041 caagtatcgt ccaccgatta ttagtggatc agttagatct caatatttcg cattttacac
     5101 cttttagtat tcacgggttg ttgatcactg ttttgttttt tgctgttttg tttttattag
     5161 ctgctgttgt caatgctttt tcgattgtca aaaaaccgat tttagcattg attcgtgcgg
     5221 atcagacacc aacaagaatc aagcagcata aattcttgtt tttccttgaa gtagtactgg
     5281 gaattgcttt tctaggctgc ggttactata tgatgaaaca cgtggggacc tatcaagtct
     5341 ttggtatagg ggtagcctta gttacgatca tattaggaac ttatttcatt ttccattctg
     5401 tgatcatttt ctttttatca ttactaaaga aatcagaaca gatttcattg aaaaaattga
     5461 acaatttcac attgtcacag ttaaactttc gtattcgtga atatacacaa atgctctcaa
     5521 tggtcgctat gctgtttgcc ttggcactgg gcgctttgac ggtaggatta ggttttaaaa
     5581 atgaaattgc agacttgaca gaaaacaatt ctggatatga tctagtgatg aataatcctc
     5641 gggcagaaga tcagcaaaaa atcaaagaac tttcaccaac actttctgct tcttatactc
     5701 aaaaagaaga tgcgggaacg atttactata atgcaagtga atttgcggct caaccattga
     5761 tcatgataaa acatgaagat ggaacaccgc cgaagatcac gaaagaaaaa gttcctgcta
     5821 aaaaaatgaa tgaactgact gtacaagagg aacttcgaag ctatgaattg ccggaacagc
     5881 aagaaaaaga aatcaaggta gtcagtcaat cagaatttga tcaactgaac ttgccacaat
     5941 ccactttaca gctagttcag gtaaaagact tccaagcaaa cctgaaacca atcgaaacac
     6001 ttgttgctca aaataaaatg aacaatccat ctttgcaaaa agtagagtac agcaaccaga
     6061 aatatgaaat gtatgaagtg tataatggtc tattctctgg ttttgaattt atgggattct
     6121 tcctaggttt agctttcttg accatgcttg ctagctgttt gatgttcaag atcttgtcag
     6181 gaagcaagag tgacattgct cgttatgaaa tgcttgaaaa gataggaaca cgacgcgggt
     6241 tgcttaaaca atcgatccgt agagaagtag gcgtgttatt cttagcacca ggaatcttag
     6301 gtgtagtcca tgtcttattc ggattacaaa tgtttggtct attcatgcaa aatccataca
     6361 aagatatttg ggttccgttt acgatcttct tcgtattgta tttcatctac tatgtactga
     6421 cgacatggtt atatactgga atcgtattga aaaagagatt gtgaaaaagc tgtcctgcat
     6481 caagtaataa gaacagccaa gcaaaaatag ctcctcatat ttttcgtttg gttgggctta
     6541 ttaccgcaga tgcaagcttt tgaacaccgt ttgttcggaa aaagaggttg tgacaatatt
     6601 tttgtcacaa cctttttttc acggtctttt attagaatat agtaaatttt ttctcgttac
     6661 tcacggaata agctgttaag aaaacggttt ccatgtatac tggaatcagt gcaaatgaaa
     6721 gaaaagaggg tgcaaggaac atggaaatca atgaacaaaa ttttggcgaa aactatcact
     6781 tgattacgat cacgaatcaa aatgggacat ctttgtctgt cagtgatctt ggggcacggg
     6841 ttgttcattt tcaaacaata atcgataatg aaaaacgaga gttgatctta ggttttgatg
     6901 aagcaaaaga atatctagaa aaagaccctt atatcggtgc ttcaatcgga cgtatggctg
     6961 gacggattac gaatggcaca ttcaaattag atgatgaaac gttcgaagta aaaacagacc
     7021 cggacactgg tcattgttta catggtggtg cacctagctt tgaagccaag cagtggcatt
     7081 atacagtcat caatggtgaa aacgaagcat cggtcatttt ctatcagact agcccagata
     7141 tgggaaacgg atttcctgga actttagatg tagaagtacg gtatacgctg acgaacgatg
     7201 atacatggcg agtgacggtc caagggatca gtgaccgcaa aacagtattc aatcctacaa
     7261 atcatgtcta tttcaattta accggtgatc cagcacagtc aatcgacgaa catatacttt
     7321 ggttgaatag ccgattattt gctacgctca ataaagatac aacgcctaca ggagaagtag
     7381 tttcagtaag tggcacagca tttgacttcc aacgtcctaa aaaattaggt gaggtctttg
     7441 ccagtgattt tcgccaatca atgatagtgg gcggcatcga tcatccattt ttgttgaaaa
     7501 aacaatctgg tttactaaat gctgcggaat tgacaagccc agatgaaaaa gtatcgatcc
     7561 aagtaaaaac agatgcttca ggaatcgtga tttttacagc aaattttgga gaagatggac
     7621 cggaaatgcg cgggaataaa ttagtaaatc atggcggtat cacatttgag actcaagaac
     7681 tgccaggagc ggagcgactt gaaagttttg gagatgtcac tctggaacct aaccaagtac
     7741 gcgagtttgt aacggaatac aaaataaata taaagaaata gaataaatta aaaagggagt
     7801 gtgacagtgt cacatcccct ttttaattta ttctatttct ttaaaggcag atcaagccag
     7861 aatgatttgt ctaaacttaa caaggttagt acaggaacag ttgcagcttt aactgtacga
     7921 gtagttgttt ctggtatctg tcttgccgta aaattgttcc acaaatccca gattgaagcg
     7981 acacggaaga tggaagaagc gttgttgacg cgttttatcg ttgaaaattc taatcctgtg
     8041 attggcacgc catctgttgt ccatttgatt ggcacatcga atcgcttaga catattgagg
     8101 atttcagcgt ttgcttcttc agttgttgct gcttcttttt tcggttcatc atattttgtc
     8161 aggtcgtacg cttcgatgat cgcgttcaat ttttgtgaat attggcgatc tgtagcataa
     8221 cgtccttcca aatatttggt tgcttctttg tagtcttttg tttccgactt ccaagttggt
     8281 ttgtagaaat caggattgcc agtcagtcca tttttgatca agtcggcata gtcttccaaa
     8341 ctttccttgt aagaaggata tttacggaat tgtgcgcgga tttggtagct ttgtccggaa
     8401 ctatcttgtt caagtgtatt gaaggtcacg ctgcttcctt tatatgatcc tttgatacca
     8461 aaaaggttat gatttggttc gcttgataat aagctgtttc cagatccact ttcaaggata
     8521 gcttgagcca tcatgactga tgcgtagatt ccttcttttt gcccgatttc ctgtgcatca
     8581 tctgcaatct ttttcaagaa ctgatcagtt gtttggtttt gactgaaatg gaacgagcca
     8641 tctaatccat ctaaaataga tgagccgcct gtcagcgaac ctgaagcagc aggtaaacct
     8701 gctaagttta ctcggacagc aaaatcaggc gtgaaatctg aaatagaagt tactcgaact
     8761 ttatctcctg gttgaggcgc atggataaat tgtccgttcc caatatagat acctacatgg
     8821 tgagtaacac cggaagaagg tccccagaag acgagatcgc caggttgggc ctgacttact
     8881 ggaatctgcg tacctgcata ttgctgttcg cctgtccagc caccgatata atgaccggtt
     8941 gctttcatat atacgtaata agtcaaacca gaacagtcaa aagtatttgg accttttgct
     9001 ccccatacat atggttttcc aagatgcttg aatgcttctc gaacgattgc ttgttggaca
     9061 ttcgttgtat ttgtaggcaa tacagcatca attggtgatt ttacaacaaa atcagaagaa
     9121 gcagaagatg gattctctat accgctttga tttgctgtat ttggacgaga tgaagcattg
     9181 ttcgttccgc cattcgatga accattgttt ggatggtttg gttctctctg agtatttcca
     9241 ttgttattcg atccagatgg gtttgtttga ttcgaagaat tcccgctgtt cgaattattg
     9301 ttattattgt ttgaattatt tgaaccatta ttattgttgt tattacccac atctggtttg
     9361 tttggcgttt ccggatctgg tgtactaggg acttcttcac taggatcagt tggtttctct
     9421 gttgaagtat ccggtccagc tttaaattct gcagtaatag ataaattgtt cgtacctact
     9481 gtaaaggtct ttgcatcata atttacttct ttggcaccat taacagcgac tgaagcaatt
     9541 tgttcacctt cagctggcgt aatagtgtaa gttaccttcg tttccgctaa taaaccttct
     9601 accgttccac tttccgtaat atccaaaggt tgctgttcag gggaatccgc tgtcgaagca
     9661 gtcagttgga tcttaccaat caaagaagga gcaattgtaa gggaatatgt ctcggctggc
     9721 ttttcatttt ctgatggatc agccggatca atttcagttg gctcggtcga tcctgttgtt
     9781 gagttagttg ttccagttgg gtcttctgta aaagaatccg ttgtttcact agtagctgag
     9841 ctggatgtag tcgtatctgt tgtgactgtt tgatcagcta tttgattatc tacggcaaat
     9901 acggtattgg tcgacaaaac agacggagca atcagccaag tagcagctag caaacgtatc
     9961 gtttttttat ttttcataat aaactagtcc ttttcctttc attgattttc tgtcttattc
    10021 agcattcatt agacattttt tggaacagat aatcgtgtcg aaaagctttt tcttttcata
    10081 aatctgtctg tgtattctaa ataaaacaat ttgtaaataa gcaacgagca ttcattcgtt
    10141 tttctttgac gttcatttta ttgcatagac cacctttgtt tggggataaa caagtgggga
    10201 taaaaatggt tatgcatatg taaattatac caaagtttgg aaagtatcac aaaaatttat
    10261 aaaaataatg atcctaaaaa gaaaaagact gaaatatgtg ataaacatct catacttcag
    10321 tctttttcat taactattca taagacatta ggtcttcatc aaacccgcta acgagagtag
    10381 tgatcattgc tacgactaga ctaacagcca gtcctgctaa gtaccagaaa aaagtaccgg
    10441 aaatgaatgc aggcaatgtg gtcagactac caaatacaaa agcgatcgct ttcacttggg
    10501 caaatcccat tacagctcca ccagcagctg caccaatgca agcagcaaca aatggttttt
    10561 tcaatgggat cgcaattccg taaacagttg gttcggtcac tcccgcaagt aaaatcgaag
    10621 caaaagcaga accggagatc gaacgggttt ttgaacgttt acttcgcaga aatacaccta
    10681 aagctgcacc agccattcca aaattggctg ctcctgtcaa cggaacgatt cggtcgaacc
    10741 cataagcaga gatattactg atcatgacag ggtttacagc ccattggata ccaaatatta
    10801 ccaaaatatt ccaaccacca gcgatgattg cgccactgat cggtccgtta atatccatca
    10861 tccaagtaat tgcggcagct aaaccttcgc caaggtaaac acccgcaggc ccgaatacca
    10921 ttaacgtcaa aggaaccgtg ataactaatg aaagtaaagg tacaaatact aattgcatat
    10981 tttcgcgaat atattttttg aggaattttt caacaaaaga ataaaagaaa atacctaaaa
    11041 tcgccggcat gacagtagac gtatactgca tcagtactac aggaatcccc agaaaatcag
    11101 tagctgcttt gccactgtct gaaagtaaag aggtcagatt cggttcaatc agtgctgcag
    11161 caatgacagc cgcaatatat ccattcactt taaacgtttt ggcagcagaa aaagcaagaa
    11221 agattggcag aaaataaaag acggcattcg acgcagcaga caaaatatga taagttccgc
    11281 ttgtttcact aagccagcca atcgtagtcg ctaaaacaac cagacctttt aatataccag
    11341 aaccggcaaa cagcggaatg atcggcgtaa agatttccga tatcaaacca aatagttttt
    11401 cgatcggatt tagcttttct ttcggagctg aatgatcgat ttgacgcgtc aattccttgt
    11461 aagcaggagc caatccctga tccatgatta tttctgcata tctatcgagt aaaatgaaat
    11521 acttaatgga agggatttgc tgttttaatt tttctgcatt ggctactggc tgttttttca
    11581 atttgatacg aagccgagtc gcacatggaa ggacagaatc aatattgtct tttcctccca
    11641 actgttcaat cagcatatga atctgctcgg acagtaccat ctgttcagga ttcattgtcg
    11701 tttccattag aagaccactc cactcactaa tataatattg gcgtaaggtg tcatttcgcc
    11761 tgtacgaata aatgcatgac agttattcaa ctcttttttc atttcttcat gtgggataaa
    11821 gctaatcggt gtatctggta acaataattt gattttttcc aatatttccg ggttcattgt
    11881 ctttatttct tccgccaatt caatcgattg tacctcaagt tcttctaaaa cgttttctaa
    11941 aacagataaa aaacttggaa gaccgtttga gacagccaaa tctatttttt cagtaccaaa
    12001 ggggacaggc atgcctgcat ccccgatact taatttatca aagtgcccca tttgtgcgat
    12061 tactcgtgaa atatctgaat tgattacttt tccttttttc atgtttgttt cttcctctca
    12121 ttgttaattt tagactgctg attgttctaa agcttcgcgg taagggatag aaggttgcgc
    12181 accataagac tgcacagcaa cagaagaggc tagatttcca taaaggatcg cttctttcaa
    12241 gttactaagg tctggctgaa gtgtgctgct taatgcgcca ataaatgtat cacctgcggc
    12301 tgttgtgtca acggcatcca ctttaaaagc agggacgata ccgtgttctt tttctgtatg
    12361 atagaaagcg ccggcactgc ctagtgtaat gattactgtt ccaataccta attcatggag
    12421 tttttcggct gctgcgacca aactgttatg atctgtcact ctgattcctg taatgatttc
    12481 tgtttctgtt tcatttggta taatgatatc tgtcttttca agtagttcag taggaatcgt
    12541 ttcacgagct ggtgcaggat tcaggatcgt tgtttttcct gcgtcttttg caatagaaaa
    12601 agctaggatc gtcgcatcta agctactttc aaactgagca ataataaagt cactgtttgt
    12661 aatcagttgc ttgtttttta ggacttcttg ttcatggaaa gcattatttg cccctgcatg
    12721 gatcaagata ctattttctc ctgctgcgtc taccataatc attgcttgtc cggttgtggc
    12781 gccttggatt tcagctatcc cagatgtatc gatgttttct tttgtcagta gatctaataa
    12841 ctgttgtcct tcgctgtcag ccccaacgcc tccaataaaa gatgtccgtg cgcctgaacg
    12901 tttggctgcc actgcttggt tggcaccttt acctccgcca gcgtgaaaaa tctcatgcgc
    12961 atgcatcgtt tcgcctggct tgggcatgtt ggttaaacga agggtagtgt ccatattaat
    13021 acttccaatt actgtaaccg tgttcatttt acaaacctcc aactatctca tttattttga
    13081 tgggaaaatt attttttaaa acgttttagt aaaacgtttt aatagttcga taaaaatata
    13141 gcacaccttg aaagctattt caagggtgca gttgaaaatc ttttttctaa tcgtacaggt
    13201 aatctttttt cttcccattc tttttctggg aattggatcc gatctaacaa taacttggca
    13261 gatgtttgtc ccaattcgaa gattggttgt gcgatcgttg tcagtttcgg cttgatatac
    13321 tcgcacatat ctatattatc atagccaata atgctgtagt cttcagggat gctttttcca
    13381 gcttcctcta atccgcgata taatccaaaa gctaattcat catttaaagc aaacacacca
    13441 gttgcagcag aatcaagaag ttctgctgtg atctggtagc caccttgttt tgagaactgt
    13501 gtaggtaaga ggatcagttg gtcgtgaggg atttggtaaa catcaagagc agatttaaac
    13561 ccctcaatcc gagcgtggac attttccggt ggattttcag gatagacaag ggcgatcgtt
    13621 tgatgtccta gactaagcaa gtgcatgccc gctaggtaac caccgcgaaa atcgtctgtt
    13681 cgtacggcat cactaaatcc ttcagatttt ttctgatcca aaacaatgaa gggccggcct
    13741 tgttttttca aattttcgtt gattgcatct gtagagacag cagaagtcgc gataatgaat
    13801 ccatccacac ctcgacgtgt gagctcggct agatattcga tttctttttg atgatcagaa
    13861 tcagcattac ataatatcgt gacgaaattc tgcttataca agatatcctc aattcctcgc
    13921 atcagtgtac tgaagaatgg attcgtgata tcaggaacga gtacaccaat tgttttcgtt
    13981 tccttggtaa tcatttgtct agcaaaatag tcaggctgat agcctaattc ttctttggca
    14041 gctaaaactt tctgaacagt tttcggactg aatttctccg tatttccatt taatatcaag
    14101 gagacagttg tgattgaaac gccggaaagt ttagcgactt ctttgattgt gatctttttt
    14161 ttcaccatgt tcatactcct atctaaatcc tgtggttatt ttcccatgaa atcaaaaaga
    14221 agtaaatgaa aatcacgtgt gccagtactt atttgctttg ttggatagct tcgattattt
    14281 gtttcgttga gcgtactcgt gcagttttcg gaaatatctt ttttatgaac aattgatggg
    14341 attctttgtc acgatcactg caagcatctt cagcaagcac gatatggtac ccgtgttgaa
    14401 aggcatctaa tgcagtagca tatacaccgt ttgttgtcgc tacaccgctt aaaatgatag
    14461 tttcgattcc acgccggcga agttgaagat caagagaagt accaaaaaaa gcacttggat
    14521 tgtatttttt gatagtcaac atattatctg tatctttcaa agaatccttc aaaagtaaat
    14581 cagtgaattc tggaggtaca gaagcagtca ttccacccat atcagtttgt tgactcaaat
    14641 aagggaaagt ggagatgtct acgttcacta aaactttcaa ggcagcggta cgtttcaatg
    14701 ttcttactaa caaatcattg tttgtcagaa tagatgctgc agtgtgtgga gagagtgtac
    14761 cgttatttac gatctctttt tgcatgtcga tcgcgacaaa agcagttttt gataaatcaa
    14821 aaatcatatt ttcctttaaa tcattcatga tgttccttct ttcaaataac aatgtttttt
    14881 atgtaagtaa atcataaatc gtttatttgt caactaaatt aaaggattcc tttctttagt
    14941 ctagagaaat tcagtggtaa aataaataag tgtgagaaaa cgcattcgat gcatagatat
    15001 agcgagaaat atcgtgaaaa tggaggaatc agagtgaagt atgtcgaagt aaaaaatgaa
    15061 tacaaacgat acaaaatggg agaaacgacc attacagcaa atgacgatat ttctttttca
    15121 attgaaaaag gcgaattagt gattatactt ggtccaagtg gagcaggaaa gtcaacgatt
    15181 cttaatattt taggtgggat ggatacacct gatgaaggtc agatcattat tgatgacaca
    15241 gatattgcgc aattttcaga taaacagctt actgcttacc gacgaacaga tgtagggttc
    15301 gttttccaat tctacaatct cgttcctaat ctaacagcaa aagaaaatgt ggaattggca
    15361 acggaagttt ctcctgatgc gcttgatcct gtagaggtat tgaggcaagt gggcctagct
    15421 catcgattga ataactttcc gtcccaatta tcaggaggcg aacagcagag ggtttcaatt
    15481 gctcgtgcac tagctaaaaa tcccaaactt cttttatgtg atgaaccgac aggcgcgctg
    15541 gattttgaaa cgggcaagca agtattgaaa ttattgcaaa acgctagcag acaacacggc
    15601 aataccgttt tgatcattac acataattct gctttagcgc ccatcgctga tagagtcatt
    15661 cacattaatg atgctaaagt aaggtctgtt gaattaaatg atcatccttc atccattgat
    15721 gagattgtat ggtaggggga ttttatgaaa acaagaacat atcttaaagc aggttttcgc
    15781 gaaattcggc aatctaaagg acgattcatt gcaatcatcc ttattgtcat gctaggaaca
    15841 cttttatttg taggtgtcaa aactgctgga cctgtcatgc aaaaaacgat ggatcactat
    15901 gttggtacag caggactttc tgatttgcaa atcgtttcta ctggtggctt gacacaaaaa
    15961 gatattgctc aagcagaaaa gatcccagat gcaaaagtag aaacaggaaa acagatctat
    16021 tattccaata ctggcaaaaa cgaagtgatt caaatatttt cttataacaa aaataacaag
    16081 cagaacaagc tacaattaac tgatggaaaa ctcccggaaa aaccaaacca gcttgtattg
    16141 gatgaaaaag cggaagatga agggtataaa atcggtgata tttatcagat tgattcagat
    16201 gaattgaaag aaaaagagta taccatcacg ggctttgtcc gttctccatt gttcatcaac
    16261 aatttggaga gaggatacgc gaacgtcgga aatggtagcg tcgattattt tgtctattta
    16321 cctgaatccg tttttaaaac agatatttat tctgtcttat atcttgattt tacaaacgta
    16381 aagaatttga atacgtacag caaagcttac aaagataaaa tggagcaaaa tcaagaaaag
    16441 gcagaaaagt atttacaaga tcgacccgaa gaacgcttag aagagctgaa aaaatcggcc
    16501 aacgaagaat tagaaccggc acagcaaaaa atagcggatg gcaaagcaca aacaacgcaa
    16561 gcaagaacac ggttagaaga tgctaaaaaa cagttagcgc aacagtcagc aactatccag
    16621 cagcttcctg aagcgcaacg aacaatcgct gttcaaacat tacctcaaca agaagctcaa
    16681 cttgctgaac aagaaaggca attagttgag aaagaacgtg aactggcctc tgcagaggaa
    16741 gaattgaatc gaaatgcaga aaaaattgaa aatttaacta aaccatcata cctatacaat
    16801 gaacgttcag aaaatgtcgg ttttcaagaa tttggtgatc tgtctgatcg tatcgctgcg
    16861 attgccaatg tttttcctgt tttctttttc tttattgccg cattgatcac ctttacgaca
    16921 atgacacgta tggtagaaga aaaccgcagg gaaatcggga cattaaaggc gttaggttat
    16981 acgaaagttg aaatcgcaaa aaaatatgca atctatgcaa gtctagcctc agggataggt
    17041 attgttttag gaacgatatt aggaacgaat cttttgccaa ggatcatttt tgagctgtct
    17101 aacgagcggt atgatattgg gagtgcgctt attttttatg actggccgcc gattttacaa
    17161 gcagctgccg cattcttcat tgccgcattt ggcgctgcaa tgatcgtttt attcaaagat
    17221 ttaagagaac ggcctgctgc attgcttcaa ccgaaagcac cgaagccagg gaaacgcatt
    17281 tttcttgaat acatcactcc gctgtggtca cgccttagct ttaatcaaaa aatcagttac
    17341 cgaaatctct tccgatataa gtcaagaatg cttatggcga ttatcggaat tgctggatgt
    17401 gccgggctga tggttgcagg cgtgggattg aaagattctc taagttctgt ttcagccaaa
    17461 caatttggac cgatcattga ctatcaagca atcgttacat tggattcatc cacggaagat
    17521 agtcgtcaaa aagcagcgga tatgctgcag aagcaagaaa aagtaaatga gatcctagct
    17581 attcatactc aaaccgtaga attgagaaaa aaaggacatg cttcgcaaag tctgacagtg
    17641 atcgtaccaa gtaaaacagc tgatttggat cgttttatcc atttaactga tcttgaagag
    17701 aacccacgct ccttgcctaa aacaggaatc gctattacgc aaaaagtagc agaactattt
    17761 gacttatcta caggagatca tatttcgctt tatgatgaag atcaaaaaga atggaaagtc
    17821 gaagtaaaag aaatcgtgaa aaactatcta ggtagcttta tttatatgtc ccctgaatat
    17881 tatgcttcag ttactgatca atcgatgatg gataatgcat ttttgctgcg tacagataag
    17941 atgacaacaa aagaagaaga aatgctttcc gagaaactgc ttgccacaga cgctgtgaca
    18001 aatacgtctt tcatgtccaa acaaatcgaa acgcaagaag aatcaatggc gaacttagac
    18061 tcggttgtct taatctttgt cgtattatca ggactgctgg cttttatcgt cttgtataat
    18121 ctaactaata tcaatatttc cgagcgtgta cgcgaattat ctacaataaa agtccttgga
    18181 ttttttgata aagaagtcac gatgtacatt gttcgagaaa atatcatctt tactctttta
    18241 ggaatcatcg gaggatttgg tgtaggatat atattgactg acttcatctt gcaacaagcg
    18301 tctatggaga atgtcatctt tccattagtg atcacatggt gggcttatgt cttatcagca
    18361 ggattaacta tcgtatttac ggttattgtg atgattgtta cccatttcaa attaaagcat
    18421 atcgatatga ttgatgcgtt gaaatcgaat gaataaaaac agaggttgtg aaatcagcgt
    18481 tttgagctaa taacgagggt cagctgctaa ttgaacacca tgaatcagag gttgtaaaaa
    18541 agccgtcctg catcgagcaa aaagaatact caaataaaat tagctcctca tgttttacat
    18601 ttgagaagac tgattgccgt agataaaagc ttttgaacac catttataga agaaacaaga
    18661 atattttgct attttaaaca agaggataga caaatacatg tctgatctct tgttttttat
    18721 tgggcaacag acctcttaga atgtataaag ataaatttta caaacgcttt ctgttcagtt
    18781 ataataataa atgataactt gtatatataa gctgtggtag gtaataaatc agttgaatgg
    18841 aggtaaatat ccatgacatt taaagacaaa gtcatttacc aaatctatcc caaatcttac
    18901 aaagacagta atggtgatgg aattggagat ttaaaaggaa tcaaagaaaa actcccgtat
    18961 ctgaatgatc taggcatcga catgatttgg ctaaacccta tttatccaag ccctcaaaaa
    19021 gacaacggtt atgatatctc tgattacaca gccatcgatc ctatctttgg tacgatggag
    19081 gaattcgaac agctaatagc cgaagcaaag aaataccgga tagaaatcat gctcgatatg
    19141 gtgctgaatc acgtttcgac agaacatgaa tggttcaaaa aagcactagc aggggaccag
    19201 cattatcagg acttcttcat tttaagagaa gagccaacgg attgggtatc aaaatttggt
    19261 ggaagtgctt ggtcgccatt tggagatacg gggaaatatt atttgcactt atatgatcgg
    19321 acacaagctg acttgaactg gcggaaccaa gcagtaagag aggaactatt caaagttgtt
    19381 cgtttttgga tggaaaaagg ggtaaaagga ttccgtttcg atgttatcaa tgttatcggt
    19441 aaagacgaac aattgaaaaa taataaagaa aatgatggca aacctgaata tacggacaaa
    19501 ccaatcaccc atacgtatct aaaagaattg aaccaagcaa cgtttgggac ggattccact
    19561 atcatcacag tcggtgaaat gagctctacg acgatcgaaa attgtatctt gtatacagaa
    19621 cctagtagag aagaattatc aatggtcttt aatttccatc atttgaaagt cgattatgaa
    19681 aatgggaata aatggtctac tcctccattt gattttgcag aattaaagcg actatttcat
    19741 acatggggag aaaatatgag tgagggtaac ggctggaatg ctctattttt taataatcat
    19801 gatcagccac gtgcgctcaa tcgatttgta gttatcgatc agtatcgaaa acaaggagca
    19861 gagatgctag caaccatgat ccatttgaac cgtgggacgc cctatattta tatgggagaa
    19921 gaaatcggca tgatcgaccc agactattca tcgatttccg actatattga cgtagaaagt
    19981 ttgaatgctt ataagttact ccttgaacaa ggattcacag aaaaagaagc gtttcgtcgg
    20041 atcaaagcaa aatctcgcga taactctcga acacccatgc aatggtcaga cacagaacaa
    20101 gcaggattta ctacagggaa accttggtta aaagttggag gcaagctaga agagattaat
    20161 gtagaaaaag aactatcttc tgaagattct attttgtcct attacaagaa attgattcgg
    20221 ctgagaaaaa catatccaat cgttgcacaa ggagattatc atgcttatga agcaaatcat
    20281 ccggaagttt atggctttct tcgccaattt gaaggacagc agctgcttgt attaacaaat
    20341 ttctatgata aagatacaag gatcgccatc ccagaaggat tcacagaagc caatattctt
    20401 atcgataatt attccgatac tgttcttcat caaagtctgg agctaaaacc ataccaggca
    20461 attgctttgc tgaaaaatta aaacaaaaac acaaaacgtc gttcctacga gctattctcg
    20521 taagatcgac gttttgtgtt ttatttaaaa taatcttctt cagaaaatga agagttttca
    20581 tactctcctt tatcgccata aaatttattg tagcgttgtt tgaatttacg gaaaataaaa
    20641 gtaacgagta atttgactaa cgtatagata ggaataccaa aaataacgcc cacaattccc
    20701 attagatcac ccatgacaag taaaacgatt aagatcgtaa tcggatggat ctgtaaacga
    20761 tcacccatta ctcgaggaat caccaagtca ccgtggagaa gttgtacggc aaaccagaca
    20821 atgacaaatt tgacagccat aaaagtagag tcttggaatg caatgatcaa acctggaaag
    20881 aaggcgataa acggcccaat atatggaatg atacataaca acccagcaga aatagctaaa
    20941 acactagcaa attctaatcc gatcagtaag aaacaaaccc agtagactgc tccaaggata
    21001 aatgaagcaa tgatttgccc ctttagaaat gaacccaatt gttgattagc aatctttgtc
    21061 aaatttttaa aatctgtacg aaatgctggt gggacaatcg ctaaaactga ctgataaaat
    21121 ttttgaggat ctctcaaaag gaaaaaagcg acaattggac cagtaaataa agtaataaaa
    21181 atagctgtaa cagttgaaaa aatgttgctg atccgttgtg cgccactttc ccaataattt
    21241 ccaataaatc cagctatatc agacgtcacg tcgtcaatag aagcaaaaaa ttgattaaat
    21301 gaatcggcaa aaggtgtttt ctccaaaaat tgtcggagac tctcttgaaa atcttgaaac
    21361 aaatctggca aactactgat caatgtcttt gcctgttctt caagaaaagg aataagccat
    21421 atgcttccaa gccccaataa aaataaaata atcacataaa cgataatgac tgacaagttc
    21481 ctcgaaaggt gcttgctcaa acgtttgatg actggttcca aaagataata caagatcagt
    21541 gcaaacaata atgggggcaa gatagtagcg ataatgattc cgacaggctt gaatataaac
    21601 gagatctgat ggaaaaaata aatgatcagc gcaagtaaga taactattcc taaaacatag
    21661 taagaggttt ttcctcctaa aaagcggatc aaacgattcg ttgtatctgt ttcttttttc
    21721 actctttttc ctcctttatc tataacttca ttatagacaa aagcgggtat aaaaggaaaa
    21781 tgatagaggt tgattgcaaa aatatcgtca atcattcata taaactttct catcaattaa
    21841 aataagaaaa cgcttgacag ttcttttaac catatgataa attaaatcct gttgctaaaa
    21901 cgtaactatt acggtttgcg aaaagcgaat cggaacattc aaatgaaaga agatgagaag
    21961 aggagtgtca taaatgaaac gattgatagg tatattggcc atgctggttt tagcaggtat
    22021 gttaactgct tgcggggcaa gtgggaaagc cgaggacagt aaagaaaaat tgagcgtgat
    22081 gacgaccttt tatcccatgt atgattttac aaaagcaatc gtcggggatg aaggagaagt
    22141 agagttattg attccagcag ggactgattc ccacgattat gaaccttctg ctaaagacat
    22201 ggcaaagatc caagatacag atatctttgt ttataatgat gaaaacatgg aaacatgggt
    22261 ccccgcaatc caaaaaacat tacaagaagg caatgtccat acgataaaag cgacagaagg
    22321 tatgctttta cttccgggaa gtgaagaagg acatgatcat gaccatgaac acggggaaga
    22381 aggccataca catgagctag acccacatgt atggctagct ccaagtttag caatcaagca
    22441 ggtagcaaac atacgtgatc agctgattga agcttaccca gaaaaacagg aagtatggac
    22501 aaaaaatgca gcagcctata ccgagaaact tcaagcactc catcagcttt atcaagaaac
    22561 ctttaagcaa gcaaaacaaa gatcttttgt cacgcaacat gctgcattca actatctagc
    22621 gttagaatat ggattgaatc aagtatcgat tgctggtctt tcttctagtg aagaaccgtc
    22681 tgcggcgaga attgctgaac tgaaacactt tgttaaagaa catggaatca actatattta
    22741 ttttgaagaa aacgccaaag atagtattgc cagaacgtta gcaaacgaag caggtgtatc
    22801 tttagaagta ttgaatcctt tggaaggatt gacgaatgaa caaatagaaa atggagaaaa
    22861 ctatctttct atcatggaag caaacctcga agcgttgaaa aaaacgacag agacagaaaa
    22921 cccgctagaa gatactttga cccccaaaaa agaaaagagc gtctacaacg gttattttga
    22981 agacgaggat gtacaagatc gctctctttc agattgggca ggaacttggc aaacagtaga
    23041 tacgtatgta gaagatggga cttttgatcc tgtttttgaa tataaagaaa aattgaatca
    23101 ggataagaca gcacaagaat ataaagaata ttatacgaag ggctatcaaa cagatattgc
    23161 ttcaatcaag atcagtgaga aagagatggc atttacgttt aaggatggaa caacgaaacg
    23221 ttcaaattat cgttatgtag ggaaagaaat cttgacctac gaggcaggaa accgtggcgt
    23281 aaggtatcta ttcgaagcag aagataagga aagtggcgct tttagatata ttcaattcag
    23341 tgatcacacg attgcaccgg ctgactccga acattttcat ttatatgcag gaaatgaaag
    23401 tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag atacaatgga
    23461 tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaa
//
LOCUS       DARBFD010000038        23239 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000038 DARBFD010000000
VERSION     DARBFD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.038"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..667
                     /locus_tag="SCK08_001412"
     CDS             <1..667
                     /locus_tag="SCK08_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1201296.1"
                     /translation="ILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISY
                     FVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASV
                     MGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPR
                     NSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDS
                     ALF"
     gene            679..1599
                     /locus_tag="SCK08_001413"
     CDS             679..1599
                     /locus_tag="SCK08_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1201297.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1640..3097
                     /locus_tag="SCK08_001414"
     CDS             1640..3097
                     /locus_tag="SCK08_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1201298.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3442..4056
                     /locus_tag="SCK08_001415"
     CDS             3442..4056
                     /locus_tag="SCK08_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1201299.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4049..5773
                     /locus_tag="SCK08_001416"
     CDS             4049..5773
                     /locus_tag="SCK08_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1201300.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5770..7221
                     /locus_tag="SCK08_001417"
     CDS             5770..7221
                     /locus_tag="SCK08_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1201301.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7694..9847)
                     /locus_tag="SCK08_001418"
     CDS             complement(7694..9847)
                     /locus_tag="SCK08_001418"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEG1201302.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9849..10895)
                     /locus_tag="SCK08_001419"
     CDS             complement(9849..10895)
                     /locus_tag="SCK08_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1201303.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11045..12334
                     /locus_tag="SCK08_001420"
     CDS             11045..12334
                     /locus_tag="SCK08_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEG1201304.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12347..15049
                     /locus_tag="SCK08_001421"
     CDS             12347..15049
                     /locus_tag="SCK08_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1201305.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15034..15891
                     /locus_tag="SCK08_001422"
     CDS             15034..15891
                     /locus_tag="SCK08_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1201306.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15962..16861)
                     /locus_tag="SCK08_001423"
     CDS             complement(15962..16861)
                     /locus_tag="SCK08_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1201307.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            17030..19201
                     /locus_tag="SCK08_001424"
     CDS             17030..19201
                     /locus_tag="SCK08_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1201308.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19269..19610
                     /locus_tag="SCK08_001425"
     CDS             19269..19610
                     /locus_tag="SCK08_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1201309.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19886..21640)
                     /locus_tag="SCK08_001426"
     CDS             complement(19886..21640)
                     /locus_tag="SCK08_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201310.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21651..22487)
                     /locus_tag="SCK08_001427"
     CDS             complement(21651..22487)
                     /locus_tag="SCK08_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1201311.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22489..23172)
                     /locus_tag="SCK08_001428"
     CDS             complement(22489..23172)
                     /locus_tag="SCK08_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEG1201312.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         7638 a         4156 c         4782 g         6663 t
ORIGIN      
        1 tatccttttg aatctgctga tctcagtcgt atttgcaatc attatgagcg agttgagaaa
       61 taaacgactc gttaaagttt atcaaacgat gtccttacta ccttattttc tatcttgggt
      121 cattatcagt tactttgttt acgctttttt aagtcctgac aaaggaatat tcaatcagtg
      181 gatcactgga catggaggag aagctgtcaa ttggtataat gaaccgaaat attggccgtt
      241 tatcttggtg ttcattggta catggaaagg gattggttat aacagcatta tttactttgc
      301 atctgttatg ggaatcgatc cgacttatta tgaagctgct atggttgatg gagctagcaa
      361 atggcagcag atcaaacatg tgacgatacc acaacttatt ccactaatga cgattttaac
      421 gattttagcc gtaggaaaca ttttccgagc tgattttggc ctgttttata acattcctag
      481 gaattccggt gcattatatg atgtgactca agttttggat acgtatatct acaatggcct
      541 gacatccaca ggagattttg gtatgacggc tgctgcagga ctttaccaat cagttgtcgg
      601 ctttgtcttg ttgatgatca caaatacgat cgcccgacgt tttgatagcg attctgcatt
      661 attctaagga ggaaaaaaat gaaaaaaaag aaagtggaga gagtccaagt ccgctctttc
      721 aataagacgg ctaatttttt ctctaatata atgattgcac tctttgctat ttcctgtatt
      781 ttgccattta tatttgttat cgccatctct tttaccgatg agtccatatt gtcgactcac
      841 gggtacagtt tttggccaga aaagttcagt atatttggtt atacgtactt gttcgaccag
      901 atgcaggata aaattttcca agcgcttttc gtcactgtac ttgttactgt tttaggtaca
      961 ctgatcaata gcacggctac ttctttatat gcttatgcga tttcacggtc aaactttcca
     1021 ttccgtcgtt tttttaccgt tttttgtttg attacgatgt tgttctcacc agggatggtg
     1081 gccaattatc ttgttatgac aaatcttctt cagttaaaag atacgatttg ggcattgatc
     1141 ttgccaatgg ctgtcagtcc cttcaatatt attgtgatgc gcacattctt taaacgttcg
     1201 gtctctgatt cgattattga atcggcacgg atcgatggag ctagcgaact gcgaatattt
     1261 gttcagatcg ttttaccgtt agctgttcca ggtattgcga caatcagttt atttgccgca
     1321 ttgggttact ggaatgactg gttcaatgct ttactataca tccaagatga taagctagtt
     1381 ccactacagt atcttttgat gaaaatccaa agtaatattc aatatctgac acaaaattca
     1441 ggagcaggaa gccaattgac aggcggatta gcctcagttc caggagaatc tgccagaatg
     1501 gcgatcgtag tcatctcgac gttgcctatt gcgatcagct atccattttt ccagaagcat
     1561 tttgtcaaag gtctaacaat cggcggagta aaagaataaa gaaacaaaaa tataaatcat
     1621 aaaattagga ggaagaaaaa tgaagttatg gaagaaacta gcctttacag gtgtttcagc
     1681 gttgttgttg ggaacattag cagcatgcgg aggttctggt tctaaagacc aggcagaagc
     1741 tagcaattcg gacactttgc agatgtatca aatcggagat aaaccagata atttcgatca
     1801 attgatggaa gtagcaaaca aacgaatcga aaaagagatc ggtgtaaaag tcaatatcaa
     1861 ttacattggc tggggcgatt acgaaaagaa aatgaacgtc atcatttctt ccggggaaaa
     1921 ttatgatatc gcctttgcga acaactatgt ttccaatgcc caaaaaggag cctttgctga
     1981 tttgactgaa ttagcgccta agtacgctga aaaagcttat aatgatttag atgaagcata
     2041 tattaaagga aatctggtca atggaaaatt atatgctttc ccagtcaatg gaaatgtctt
     2101 tgctcaacaa gtacttactt ttaacaaagc tttactagat aaatacgatc tttcaattga
     2161 tggaatccaa tcttacgctg atgcagaaaa agtcttgaag gaattccatg aaaaagaacc
     2221 aaacacggca gcttttgcca ttggacaagg cttcaaagtc caagggaact ttgattatcc
     2281 tttagggaat acgttaccat ttgctattga tctggatgga gatgattcga aaatcatcaa
     2341 ccaatacgat aatgaaacgt atatcggact gttacgtaca atgcatagct ggtacaaaca
     2401 aggactcatt ccaagtgacg ctgcaacgag caataaggat tatccactag aaggaaatac
     2461 ttggctaatg cgtggagaaa cacaaggacc gtatgattat ggcgatacaa ttttgacaaa
     2521 tgcagcaaaa caagaacttg tctcaaaagc aattactgtt ccactaaaat ctacagctca
     2581 agcacaaatg gcaaattttg tcgtatcaaa tacttcaaaa aataaagaaa aatcagtaga
     2641 acttctaggg ctgcttaaca gtgatccaga attattgaat gggcttgttt gggggatcga
     2701 gggagaagca tgggaaaaag tcgatggttc agatgataaa atcaaattgc tagatggtta
     2761 tcagccaaat acacatatgt ctgcttggaa tacaggaaac aacaagattc tatatacaca
     2821 agagtctatt acggatgaca tgattgccaa acgtgatcaa tcgattgctg atgcagaaac
     2881 ttcaccgatt ttaggattca gcttcaacac tgacagtgtc aaaacagaat taagtaatat
     2941 ttctaatgtc atgaatcaat atttagatgg attgaataca gggactgttg atccagatga
     3001 aacattaccg aaattgaaag acgctttgga caaggctggc tatgataagg tattgaaaga
     3061 aatgcaaaaa caatatgatg agttccgaca agaataataa tgaataaaga caaaggggtt
     3121 gtgacaaaag acgtgtcaca acctcttgtt ccgaataaac ggtgttcaaa agccagtttc
     3181 tcggtattaa gctaaatata tgtaaaaatg tggaaagcca tattcacaaa atttttctta
     3241 ctactcgaaa ctaagcggct atttcacaaa ctcttggtaa acggtgttca atcagctaac
     3301 cctcggtatt cgctcacaaa acgacaaaat atgaggaact atttttccgt ttgtttctct
     3361 aataatcagg tcaaggtgct gattgcccaa cctttttttg tataatgaga aagtataaac
     3421 ttaatgtttg gaggagaaga tatgttaggg aaggttttgg aagagttatt tatccggatc
     3481 tgggtcatta taaaactaac cttgtatttc tggatatata catttgccgg cgggatcatc
     3541 tttggattag gtgcggcatg gaaaacagtc aatgaactat tttatttgta cggattcgag
     3601 tataaagaaa tcacaataaa aagaggctgg aatatatata aaagaaattt tttaagaggc
     3661 aatctgctat ttagtttatt tttatctggt actgctttat tgagttacaa tttatattta
     3721 tctgttcaaa tcaaagggct cattttttta gctattgatt ttattttact ttttgccttg
     3781 ctctgcctat ttgctactta tcaatacagt atgattttag atagtgaata tacgatctct
     3841 gttcctaatc tgctaaagct aagttttatt tcggtttttt ccagcttttc gtcttttcta
     3901 aaaataatta ttggtagcgg tataatttta ggcgtgactt ggcaattcaa aggattgatc
     3961 ctgtttggtg tcattggtct tttgacggtt tggaatggta cgatgacaac acattggcgt
     4021 gaagaattag ataaacagtt ggaaagttat gaataagttt cggattctat tttcaaaagg
     4081 aaccttacta aatcgtctgt tgcggaaata cgcggttatc atgatcggtg taacgatgac
     4141 tgctaccgtt atttttagta tccatacttg ggaacaaaat caaaaacagg cagaaaacat
     4201 gacaagtgat gccgttcaaa gcacaagcag aatgctgaat gataaaacaa ccttgagtag
     4261 gatcatcaaa aaccagcttg tagggaattc cgaaaagatc gaaaatgtga cgacctactt
     4321 aacaaaaccc atagatcagt atctgatgta tgtatacgaa cagcaaaaca gtaccgatga
     4381 attagtttct ttcccaaatc aaatcaaaga tctctacgca aattatgaag aactaagtgc
     4441 gatatatatc gtgttgaatc aactaccgga gtattatgaa tcttctagag aaaacaaagg
     4501 tggggaaata aaaaagggga caccaaaatt actagacgcc ttttatatca agttgcctat
     4561 cacacaagga ggagctgagt caggaagtat ttttattggt tttcaaaaga aagaactaga
     4621 tatgatttta gaaaatctaa cgtcattcaa tggactctct ttatacatga tttctggaac
     4681 gacaaatcga ctatacacat tccatgataa taaaatagct aaagaaacgt ttgaaagaca
     4741 agaaatgatc atcacagaga gtctgaagaa aaattcacaa ttgccgattg acgatctgga
     4801 aaaaaacaat tttgttcaac accaagaact gtctgatgat tatcgtatat tagctgtact
     4861 agataaaaaa agtgtaaaaa tcgaaacggg aaaaaatctt gccccgctga tcattggtat
     4921 cattatttta gacaatatac tgcttatctt tctttataaa acattcaaac gatattcaca
     4981 gcaagtatct atcgtcatgc aggccatgga tcaaacagca gcagggaatt tagagacgcg
     5041 gatcgacata acaaacacgg aatatgaact gagggaactt tcaatcggta tcaacgaaat
     5101 gttggatagc atcaatcagt ttgtagaaga catctacaaa ttagaaatca aacagcaaga
     5161 cgcacatatg cgggcgcttc aagcacagat caatccacac tttttatata atacattaga
     5221 atatatccga atgtatgcaa tcagcgaagg aagtgaagaa ttagcagatg ttgtgtatgc
     5281 gttttccgct ttgcttcgaa acaatacgaa tcaagagaaa acgatcactt taaaagaaga
     5341 actggacttt tgcgaaaaat atgtctattt gtatcaaatg cgttatccta atcgagtagc
     5401 ctatcatttt atgatcgatc ctgatttgga aaagatcgaa gtacctaaat ttgtgatcca
     5461 gccattagtt gaaaattatt ttaaacacgg tattgatttt actcgattcg ataatgcgct
     5521 aagtgtcaaa gtcttgcagg aaggcaaacg ggtaaggatc atcatcaaag ataatggaaa
     5581 agggatgact gaaaaacggc taaaacagat tgaagaaaaa ctgtctcatc ctaaagtgga
     5641 acttcacggt tctatcggtc tgcaaaacgt gaatgaacgt ttacgtgcca gcttcggcag
     5701 cagttattat atgtcgcttg agaataatga gacaggtgga ctgaccgttt ctattacatt
     5761 caaggaggga tgagcgtgta tcaagtgtta ttggtagatg atgaatacat gattttgaat
     5821 gggttaaaaa agataattga ttggcaaagt ttaggctttc aaatagtagc aacagcagag
     5881 aatgccaaag aaggactagc tgtacttgaa cagcgacaga ttgatttagt agtaacggat
     5941 gtgacaatgc cagaaataaa tggactggaa tttattgaag cggctcaaaa agaacgtcat
     6001 aattttgaat ttatgatttt atcaggatac caagaattcg attatttgaa aggaggtatg
     6061 caattaggcg ccgtgaatta tttgatgaaa cccgtgaata agttcgaatt ggttgaaagc
     6121 ctcaagaaaa tcaaaacacg gttagatcag caaaatgaac aaaaaaatca acaagaaatc
     6181 tatcaagaac tgttattcag tcaatggttg aatgaagaat tggatgaaat aagtgaagaa
     6241 gaaataatag gacaaataag tgagaagcag cataggatag tattgattca actggcaaga
     6301 actcatgata ctcttatcaa tagatggttg caagaacggc aacaaatctt ttactatcaa
     6361 cgaaattacg gagaccgtgt attgatcacg ttgttgttgg aggcagatga agttgatggc
     6421 ttctgttgtt ttgttcaaga aaactttcct gatcaggaat ggctgatcag tatcggggaa
     6481 gagacagatt ctttggacaa aatccctgaa acattccaac aagcgaaaga caatctacag
     6541 ttgcatcaat tttatggaga taaagacgag catgtttttt acgcagtaca agcagaaaca
     6601 aaagatcaaa ccatcgattt ttcaagtttc agacgcgtgc tgcaaaataa acaattggaa
     6661 gtcgctcaaa aaatgatcac tgattttttt gaacagtttc agttagcagc catgatgccg
     6721 gaagatatac gatattccag ttttttactg tttatggaga ttcaacggga attgattgat
     6781 ctggaagatg aggaatattt acaaggaatc gaaaaaatcc atcaggcaaa aacggtgcaa
     6841 gaacttcagc agctacttct gtcattcatc aaaaagcatc agagacagaa aaagtacagt
     6901 gacaatgtgg aaaaagtaat cgtaatcctg caccagcatt atcaagaacc acttacactc
     6961 aaagaagtgt cagaaaacct gcatttaaat gtcatgtatc taggacagct gttcaaaaaa
     7021 gaaacgaaaa aaagtttttc agcttatttg aaccatttac gtatggaaaa agcgaaacaa
     7081 ctgcttttgc acagtaatca aaacatcaat gagatagcaa gtgagattgg ctacaacaac
     7141 accacgtatt tctctaaatt attcaagaaa atcgtcggca gatcccctaa agagtaccga
     7201 gaaaacagtg gagaatcata aaagaaaaag ccaatgtaat gaaataactt ataaaaaatt
     7261 gtctagaaat ctaattttgg gctgttctta ttacttgagg caaggcagct ttttcatgac
     7321 ctctcgataa acggagttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
     7381 tgtggaaagc cattttcaca aaatttttct taatactcga aactaaacag ctttttcacg
     7441 acctctccac taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaatggcaa
     7501 aatatgagga actattttgc catttgtctc tctaatactc aggtcagaac gctgatttca
     7561 caacctctca ataaacggtg ttcaaaagct tgcatctgcg gtaataagac caaccaaacg
     7621 aaaaatatga ggatctattt ttgcttggct gttcttatta cttgatgcag gacagctttt
     7681 tcacaacctc ttgttaatta tagtttgata aggaaaaagg caaatctttt gcttgatatt
     7741 tcttagggtg agggatgatc cctaattggt aagagatcgt gccgcctttc agcaaatctt
     7801 gatgggtaaa atataatttc gtatattctc tgtccatccg tttgatttta tggataaatt
     7861 gttgctgcgg ccgattaggc gttgcttcaa tcgtcaattc ctttccattt gacaaaagca
     7921 aaacagcctt gtcaattaat ggcataccga taacatattc accggttcca ggcgtgacag
     7981 gataaaatcc cagactattg aagatatacc aagcggccat acttccattg tcctcatctc
     8041 cagggtaacc cattggagaa tcgtcgaagg tctgagtcat caactgttta agcagtggct
     8101 gtgccatttc aggttgtcct atataactga acaagtaagg atagtgaaaa cttggctggt
     8161 ttgaaatagc taattgtcca aattctatcg cggccatctc actcatttcg tggatctcaa
     8221 aaccatatcc tcctgggtca aacgttgggg gctgattgca cagctcaatg agtttttctt
     8281 tatatctctc actgccacca tattttccga tcagtcctga aaaatcgtga taaacagcaa
     8341 agctgctttg ccaagcactt ccttctgcat aatcttgtcc ccaacgatta gctgaaaatg
     8401 gttctctgaa cttgccatct ttatcttttg aacgcataaa tcctgactca tagtcaaaga
     8461 tattttgata gttcaatgat tgctgctgat atttcttcgt catttcctct tcttctagta
     8521 ccttggcaac ttggctaata caaaaatcgc tataagcata gtctaatgtg tgattgactg
     8581 attcgtggta gtgattagga acatagccat atgtcaagta atcttctgtt ccttggcgtc
     8641 cataatttgg atgtttgctt tgaaccgtag cacctttttc cattgccgtt agaaattcag
     8701 gcatcaagtc tcgtcggata tcttttacag cagcatctgc aatgaccgca tcgatcaatg
     8761 tcccaggcat aagtccacgt tcatcaggag acagccattt tggcaaatag ccactttctc
     8821 tatagctgtt caaaaaacct tccagcattt cttcgtattt ttctacagca atcaaagaat
     8881 aaagaggata aaccgtttta tacgtatccc aaaacccatt atttgtatag agaacaccag
     8941 gttttataga tcgactagtc gtatcatagt ggattttttg cccactagga tcaatttcat
     9001 aaaatgtttg tggaaataaa aacacccgat ataaattatg ataaaacgtc cgcaattgtt
     9061 gttgatcatg gtgtgtgagc tgtatacgat caaaatagga ctgccattgt tcttgactgt
     9121 gagcaaggta agtctctttg ctttggtttt gttctagttc caggttataa gctgcttgtt
     9181 ccgagctgat aaaagatgtc ccaaatcgga tagtttgttt ttttgtctgt tcaaaataca
     9241 acgggatcga gtcgctattt ttgttggaaa cagctaactc atcaattgtt aaaggatatt
     9301 caaatctcaa tataaaataa aaagaaaaat caggatcttc tgaaccagca aaatgtgtga
     9361 tttttccttc cactgtgtaa ggatctataa caaagagctg atggcgtccc ggaaaggata
     9421 aaagtaaacc gctgtcattt ttgctgtaat caatagataa gattccgcca tacatgctag
     9481 gtatcaatgt agaacagatg ccataacgta attgacagat cgacagatga gtcgggcaaa
     9541 atatactttc ttctgggcgg taagaacttt gagcatggaa aagggtgttc tcctgtagtt
     9601 ttccattgat cggcgtaaac agcatatgac tgaaatcacc catccaagga cttggttgat
     9661 gggtaagacg gtagccttga aaaacgcggt catcaggatg aaaccaccag ctgccgtttt
     9721 gatccgtagt ttgcggtgca aaataattca tcccaaaagg aacgccagta taaggctggc
     9781 agttcccatt agagaaattt ggctgattcg ctgttgcatg tctagtatct atttgaccga
     9841 tattcatttt atttctcctt tgttgattgt cttttgatga atgaaactgg aaatttacta
     9901 tcggatggtc gatcttttgt ctctaaccaa gtgaccaaag catctcccgc aagcttacct
     9961 aactgttcaa aatcttgtgc gatggttgtc agtggcgggt caacaagagc tgctgcttgg
    10021 atatcatcaa atccgataat tgaaaaatct tcagggatat gataaccatg ctgtctcaag
    10081 agattcatgg tctggatagc gacaacatcg ttttcacaaa caaaagcagt tacttcatac
    10141 ttagtgatat aagaaagcaa ttcatctaat gtcgcgcttt tgtcatccag tgaagtgtgg
    10201 aaagtcaaac cagctttgtc caatgcttca aggtaaccaa ggtaacgctg tcgaacggat
    10261 tgtggatgag tctgcgtacc aaacaaataa gcgatttttg aatgtccctg ttcgatcaaa
    10321 taagaggcag caagcgcacc gccttggaaa ttatcggaaa gaacggtagg aaaaggtaac
    10381 tcatagattt ttttatctaa tatgacgaca gggaattctt tcaatgaaag ctcaaaaagt
    10441 aagtcaagat gttgttctgt atcaagagca taataaatca atccgtcaaa ttcattgatt
    10501 agctcttttg ctgttttatt ttgtagaaag tcaagcgtcg tcatcatgac atcaatctgt
    10561 ttatcttgca tcacagggtt caatcccttg ctaaaatcac caacagacaa atcagcgaca
    10621 tatggaagaa gaaacagaat ccgtcgtgtt tttgtaggag aagcaagaat ctgttccgga
    10681 gaagcggatt taacgaaact gcctttcccg cgaacgcgat aaataagtcc taattgctct
    10741 aattcagtca atgcacgttt ggaagtgatt ctgcttactt tataggtacg tgaaagttct
    10801 ttttcagttg gtagttggct gtctacaggt aattcaccag ttaaaatagc cgtttgcaaa
    10861 tcattaaata ttgtctggta caatggttga gtcatataaa cacctcttaa tgatatatca
    10921 tattcaaata ataccataat ggatgaaaaa gtaaataaaa agatattttt tgaaagcgta
    10981 gacataattg gctgtatgaa gtataatctt taaagatata tcaaatagaa agaaggtttt
    11041 attagtggtt tataaagata taccaaaatc ggtgcagaaa tttatggaag atatcacaca
    11101 aaaatgtggg gatatccatg cggactgggc aaagaatttt acagcagcat ttgccaatac
    11161 tctgttgaca acagtcaaaa agcaaaatga cggaaccacc tttttattga cgggagacat
    11221 tccagcgatg tggttaagag actctactgc acaagttcgt ccttatttag tgattgcaaa
    11281 agaagatgag gaattagctg acatgatcag cgggttagtc aaaaagcaat ttttttatat
    11341 caatatcgat ccatatgcga atgccttcaa cgaagaagct aacggtgctg gccaccaaac
    11401 agatcataca aaaatgaacg attggatctg ggaaagaaaa tatgaaattg attctttatg
    11461 ctacccaata cagttggcct atcttcttta caaaaataca ggaaaaactg atcaattcaa
    11521 tcaatctttt attgaaggta tcaaaaaaat cttggacgtg ttcaaaacag agcaagatca
    11581 tgaacactct ccttatacat ttgtacgaga cacgacaaga aaagaagata cgctgatcaa
    11641 tgacgggcga ggtgcaaaaa cagcttttac aggaatgaca tggtctggtt tccgtcctag
    11701 cgatgatgct tgccgttatg gatatctggt accatccaat atgtttgcgg tggtcgtgtt
    11761 aggttatgtg gaagaaattt ttcaaacgat actcgatgat aaagaaattg tacagcaaac
    11821 acagaaattg aagaaagaga tccaagaggg gattcagaat cacggacgaa cggttaatca
    11881 aaaagggcaa acgatttatg catatgaagt agatggaatg ggaaacgcca cagtcatgga
    11941 tgacagtaat gtgcccaatc tgatcgccgc accttacttg ggatattgtt ctgaacaaga
    12001 ggaagattat ctgcaaacaa gaagaacatt gttgagtaaa gaaaatccat atttctatga
    12061 aggaaagtac gcaaaaggaa ttggatcctc ccatacgcca gaaaaatacg tttggccgat
    12121 tgcattagct atggaaggaa tgacaacgtc agacaaatct gaaaaagagc ggatattgaa
    12181 tttacttgta gcaactgacg caggaacaca ccttatgcac gaaggattcg atgtgaacaa
    12241 tcctgaaaat tatacgagag aatggttttc ttgggcaaat atgatgtttt gcgaattagt
    12301 catggattac tttgatatta gagtagaaaa gtaggaggta tcgtaaatga aaaagaaagt
    12361 atatatcatt tctcatagcc actgggatcg tgaatggtac atggcttacg aacagcatca
    12421 catgcgactg attgaattga tagacgatct actagagcta tttgaagttg atccgtcctt
    12481 caacagtttt catttggatg ggcaaacgat tattttagat gactatcttc aagtaaggcc
    12541 agaaaaaaga caggcaatcc aacaagcaat caatgaagga aaattgagaa ttggaccatt
    12601 ttatatcttg caagatgatt ttcttatcag ctcggaatcg aacgtgagaa atatgctgat
    12661 tggcatggaa gaaagccgta aatggggaac ccctgtcatg ttaggctatt ttcccgatac
    12721 atttggaaat atgggacaga cgcctcaatt aatgaaacaa gctggtattt cagcagcagc
    12781 gtttggccgt ggtgtaaaac caattggctt tgataatcaa gttttggaag cagaaaacta
    12841 ttcttcccaa tactcagaga tgtggtggaa aggccctgat caaacagcaa tctttggtct
    12901 gctatttgcc aattggtata gcaacgggaa tgagattcca gtagaaaaag aagcagctct
    12961 tgccttttgg aagcaaaaat tagcggatgc agaacaatat gcctctacga accacttatt
    13021 gatgatgaat ggtgttgatc atcaaccggt acaaaaagat atatcaaaag caatccatct
    13081 cgcaaatgaa ctgtttccag attacgagtt tattcattct aattttactg actatctgga
    13141 agcagttcaa aaagatgtgc cagaagactt aggctcagta gaaggagaat tgacaagtca
    13201 agaaacggat ggctggtata ctttagccaa tacagcatct gctcgggttt acttaaaaca
    13261 atggaatacc aaggtccagc gacagctaga aaatattaca gaaccacttg cgacgatggc
    13321 ttatgaagtt agtggaaact atccccatga tcaattagat tatgcttgga agacattaat
    13381 gcaaaatcat cctcacgaca gtatttgcgg ttgttcagtc gattctgtcc atcgggaaat
    13441 gatacctcgt tttgaaaaag cagatgaagt tggaaagtat cttgcacaag attccttaga
    13501 acagctgacc gcagcaatcg atacgaccgg attcccaaaa gacagctttc catttgtcat
    13561 tgtaaatact gcaggaatgg ataaaactgg tgaagcagaa ataaccatcg aattagaaag
    13621 aaaaagattc gctgaaggaa tacctgaaca actatatcaa gagttagaaa atttgcctaa
    13681 acgtaagtat catgtagaaa caaaaagtgg ggccactata ccagctattt tatctgaaga
    13741 aactgtccaa tttggttatg atttgccgaa agaccgtttc cgtgtgccat atatggcaag
    13801 aatgatcaaa gtgactttac cattagaaaa catgccagca ttttcttggg aaacattcgc
    13861 gcttgtagaa ggggaagcga aagcagaaga aaaggaaaca atgattcatc agagtggacg
    13921 aatcatcgaa aacggccctc tacatttgac gattgaaaaa aatggtacca ttacaatgga
    13981 agatcgcaaa aacaaacgaa agttgaataa tctgcatatt tttgaagata tcggcgatat
    14041 aggaaatgag tatatcttta aacaaccttt ctgtgatcag ccaatcctat ccagtaacaa
    14101 agaaaactct gaagtaaaag tattagtaga tacaccggaa atagcaaaaa taagtattct
    14161 acaggaaatg gaaatccctg tttctgcaga cgagcgttta gaaaaagaac agcagatggt
    14221 tgtggaattt cgttatcgaa aagcagaacg atccaaagaa aaacgaatcc ttcaaataaa
    14281 aacaatcatg acaatcagaa aagatagtaa aaaaatcgac tttgaaacga ctctcgacaa
    14341 tcagatgaaa gatcatcgct tacgtgtctt gtttcctaca aagttacatg tggaacatca
    14401 cgaagcagat agtatttttg aagtggtgaa gcgcccaaac catgtgtcta aaagttggga
    14461 aaaccccacg aatccgcaac atcagcaagc atttgtcaat attcatgatg aagagtacgg
    14521 tgtaacagtc ggaaactttg gtttgaatga atatgaagta acagaagacg gacaaatcgc
    14581 agtgacgcta cttcgcagtg tgggagaact aggcgattgg ggatattttc caacaccaga
    14641 agcacagtgt ctaggtgagc atcggttcaa ttacagcatt gaactacatg gaccagaaga
    14701 aaaattctct acttatttgc atgcatacgc agcacagatt cctttttcca ctcaacaaat
    14761 caaacatcat gaaggaacat tgatttcaaa acaacaatat ttaacgataa aaagtgaaac
    14821 gtttgcaatt acagctttga aacgcagcaa gtttagtgat aaagtagtag taagaggttt
    14881 caatatgtct agtcatttag aaaaattgga aataactaaa gacaatggaa aaactgtcat
    14941 attgaatctg cttgaagaac caacgaagca agcggttgtc ccaataatcc aaccttatga
    15001 gattcggaca atcggattcg aggaggaaaa cgaatgtatg gcggaataga agcaggcgga
    15061 acaaaatttg tgtgtgcggt ttcagatggt ggagaaattt tagaaaaatt aagtatccct
    15121 accacaacac cagaagaaac attggatcag gtatttgaat tttttgatcg ttatgaattg
    15181 aagggaatcg ggattggctc attcggcccg attggtatcg acaaaacaaa agataattac
    15241 ggatacgttt tggcaactcc caaaaaaggg tgggcacaat tcgattttct tggcacaatc
    15301 aaggaacgct acaatattcc tgctgcttgg acaacggatg tcaatgctgc tgcatatggg
    15361 gaattgaagc aaggagcagc acaaggaaaa aatagttgta tttaccttac cgtgggaaca
    15421 ggaatcggtg caggtgtcgt gatcaatgaa gaaatttttt cagggatcgc acatcctgaa
    15481 atgggacata tttgggtgaa acgccatcca gatgaccatt atgaaggtac ttgtccttat
    15541 cataaagatt gtttagaagg attagcagca ggaccgtcta ttgaggcacg cacagggatc
    15601 aaagggcaag atctgccgca agatcatcct gtttgggcta ttcaggctta ttatattgct
    15661 caagcactga tcaactatac gttgacgtta gcacctgaaa agattatttt aggcggtggg
    15721 gtaatgaatc aggatcatct ccttcaaaaa atccgtcagc aatttgttga attgatgggt
    15781 ggttatatgg aaacacctca agtaagtgaa tatatcgttc gttggggact gccgaatgaa
    15841 agtgggatta tcggtagttt gctgttagca gaacacgaac atcaaaagta agcagacaaa
    15901 ttacacaaaa agaacagagt cttatcatta tctgctgata agactctgtt cttttttatt
    15961 tctattccca taatccaggt aaagctttac cgactatttt ttcttgagta aatggatgga
    16021 tcatgtgcag ttctaatgca tggagcatca gtcgttcagc tgttctattt tggtagagag
    16081 ggtctccaac gatcggatgt cccttatgag ctaaatgaac tctgatctgg tgtgtacgtc
    16141 cggtttccaa aacacaatag acttgtgttt gttcagaaag tactttgttg acagtgacat
    16201 gtgtcaaggc gctctgacca tttttatcat ctacgaccct ttttctccga tcatggcgat
    16261 ctcgtccaat tttattggtg atggtcatat ctttggcaat ttctccccat acgatggctt
    16321 gatatctccg atagatcatt ttttgttcca acattcttcc aagtatcggc aagacaaatg
    16381 gattttttgc aaataaaacg gcaccgcttg tttctttgtc caagcggtgg acaacgtaag
    16441 gtcgttgctg tttagtgtcg agataagcag ctaaatgatt gagtaaggta ctgttttctg
    16501 tcggttcatt tggatgagtc ttcattcctg caggcttatc cactataatc aaatgctcat
    16561 cttcatataa aacacggatg tgcttcgcat ctcctaattg aacttcttga taattataat
    16621 ctgtttcttc gaataacaat gtgatctggt cattgccctt tacttcatga tggaacaaag
    16681 caggttgttg attgatccaa acgtttttac gtatacgcaa aaagtgtcga acctttctag
    16741 gaactaacca ttccttttca agcaagtcgc gaattgttgt ttcaggatgg ttcataggta
    16801 aagtgatcgt gattttcaca agtgtactcc tttcagtgca attgcagact aattgtacca
    16861 tttttaaaac atatttaaga aaaaacttac atttttctgt tcattcttat gttactctac
    16921 ttgcactaac gtaaaaaaac atattgaact acaacttaag caggaagaaa acactctttc
    16981 actatggtaa aataagacga ttgaatcaaa tagacaggag aagaagcaaa tggattttca
    17041 aagcatttta gaaaagataa aagctgcttt gatcagtttt tggcgttgga tcagaccata
    17101 tctcggacaa ttccatcgat ggcgaaagcg catctggaaa aaatatcatg tgaacaaatt
    17161 gattttgctg attggtttga tctgtgtctt agtgacaagt atctacctct ttattttagc
    17221 aaaacaagca aatgtcgaaa cgttaaaatc aggattaagc caatcgacag tcatttatga
    17281 taagaaaaac gaagaagctg gcacactgta cgcccaaaaa ggaacatatg tagaattaaa
    17341 cgcgatttct ccctatatcc aagatgcagt aatctcgaca gaagatagga atttttacga
    17401 gcatcacggg tttgatatca aagggattgc ccgtgcagca gtccgaatgg tcttgtctgg
    17461 cggaacatca gggggcggag gaagtacgat cactcagcag cttgccaaaa atgcgtatct
    17521 gacattagac caaacgtttg atcgaaaagc gaaggaatta tttttggcaa tcgagatcga
    17581 aaagaagtac agcaaagaag aaatcttgac catgtatctt aacaatgcct attttggcaa
    17641 cggggtatgg ggtgtccaag acgcttccag aaagtatttt ggagtagatg caacgaatgt
    17701 tacgctgtct gaggctgcta ctttggcagg aatgctgaaa ggtcctggta tttataatcc
    17761 gatcgatcac ccaaaaaatg cgaatgatcg gaaaaacaca gtactaagtg taatggaaga
    17821 aaacggaaaa atcacaaaag aacaagaaca agcagaaagc cagacagata tcagcgcgta
    17881 tcttaatgac acatacgata attcagattc tggttatcgt tatccttatt attttgatgc
    17941 agtgatcgat gaagcagtta acgagtatgg tcttgatgaa gaagatgtca tgaataaagg
    18001 ctataaaatc tatacagctt tagatcagga ttatcaaaag caattagaag ctacttatca
    18061 aaacagtgca ttattcccag caaatgcttc agatggtgcc atggtccaat ctgcttcagt
    18121 agctttagat ccgaatacag gtggtgtaca agcactagta ggacgaagag gagatcatgt
    18181 ctttagagga ttcagctttg ctacacagat gcgtcgttca ccaggatcaa cgatcaaacc
    18241 attaagcgta tatacaccag cactggaagc tggatacaag ccttccagta tcttgaaaga
    18301 cgaaccgcaa agttattacg atgcgcataa ttttgacggg acataccaag gagaagtacc
    18361 gatgtatcaa gctgttgcac aaagtttgaa tttgcctgct gtatggcttt tgcatgagat
    18421 cggtttacaa aaaggatatg ataaagcaga agaattcggc ctgcctttag caaaatctga
    18481 taagtattat ggcttagccc ttggtggact ggaaaaagga gtatctccat tgatcatggc
    18541 gggtgcttac agtgcatttg ccaatgatgg tcagatgtat acgccgcatc ttatcacgaa
    18601 aatcgttgat tcgactggag cggtgatcgt cgataacaca aacaaaaaac caaaacaggt
    18661 catatcaaag gaaacagccg atgaaatgac cagcatgctt cttggtacat tttcaaatgg
    18721 aacagcggct gctgcgaatc ctgcaggtta tacgatagca gggaaaacag gaaccacaga
    18781 aactaatttc gatgccacaa aagcaaatga ccaatggatg atcggttaca cgccagacgt
    18841 agtgatttcg acatggatgg gttacgaaga atccagcaag ctgcactatt tggaagggac
    18901 aagtggaaat gttgtcggga aagtcttcaa atcagcggca gaaggcatcc ttccgtatac
    18961 ttcaaaaaca ggatttagtg tagcagatgc ctatgccaca ggaggacaag tggtagctgc
    19021 agatcaagtt ggaaacacag gaaattctaa tggagagtct ggaaattggc aagataactt
    19081 gaatcaatat ggtgaacaag caaaagaagg gctgaaaaac ttcggcgata tggtgaaaga
    19141 aggcgttcag ggaattggtg aagcggctaa agacctctgg cggaaatatc aaggagagta
    19201 gcccctttga aaacgaataa ccccatgtta caatagagag taactactaa aagaaaagag
    19261 ggcataacat gtcaaacatc tacgatagcg cgaatcaaat cgaacgtgag atccgtgaat
    19321 taccagaatt caaagcgttg aaagaagcgt acgagaaagt acaagcaaac gaagaagcac
    19381 acaaactgtt caaagacttc caagcgatgc aaatcgaatt acaagaaaaa caaatgagtg
    19441 gacaagagtt cagcgacgaa gatgcaatga aagcacaaga aatggctgtg aaaatccaat
    19501 cagaagaagt aatcaatgac ttgatgcaaa aagagcaagg cttcagtaca atcatcaatg
    19561 acttgaaccg tatcatcatg acaccagttc gcgaccttta cagcgaataa aactatttta
    19621 tgaaacaaca gagggtgtga aagagacgtt cggccttgag cgatgacaaa attgcttctt
    19681 attgccgata ggctgtctct tgaacaccat ttaccaagag gtcgtgagcc taccgtttaa
    19741 ctctgagtta caaggaacaa tttcaaaaaa cagcttctca tgtttgatga ctattgtgac
    19801 ttgtgaccga ggagttggca ggagaacacc gtttattcag aaataggagc tacgacaaaa
    19861 gtcttgtcat agctcctatt ttttgtcatt catttttcaa gcggtagaaa tagccttttt
    19921 gagctaacag tttttcgtaa gtacctattt caacgatttt tccttcatcg atcacaacga
    19981 ttttatctgc ctctttgatc gttgaaagcc gatgagccac aataaaagta gtgggtgttt
    20041 ctgtcaggta attcagggct tgctggattt ttttctctga ctggttatct aaagctgagg
    20101 ttgcttcatc tagcaaaatg atttttggct gacgtatcaa cgctcgtgca atggagatac
    20161 attgcttttg gccaccagac agcttgttgc catgttcgcc tactaaggta tccagaccat
    20221 caggaagctg gtcaatcagt gaagaaagtt gcgcagcttc gatgacttca tctaaacgtt
    20281 cttttgatac gtttttcaag ccataagtga tgttctcttt gatcgaagcg gagaacaaca
    20341 aagtggtttg aggaacaacg gctaaataat ttcgcacact tcgcatatcc agttcatcca
    20401 aaggctgatc atctagaaaa atttttcctg aagtcggctg gataaatcca atcaataagt
    20461 taaggagtgt agattttcca gagccagatg gcccaacaaa agcaattgtt tctccagctt
    20521 tcacctctag agaaaaatga tcgataatcg gtttgagtga gtccggataa agaaaagaaa
    20581 catcctcaaa acgaaaattt ccatccaaat gttggatttt tttcttaccg tgataagttt
    20641 ctacatcgcc agcaatcaac agttcgccaa tagaagtgat agattccatg ccttttgaaa
    20701 tcgtaggaat cagcgtgatc attccagaaa cggaattgac gatcgtcgtg aaatacgtct
    20761 gatacatcac gacatcgccg ggttggatgc gcccttgtaa agctaatatc ccagtgaaaa
    20821 tcaaacaaat cagctgaaat atctgaaaac tagcccagct gactgatccg aaaacagatt
    20881 gcaggatatc taagtggaat cctttttcat agatttgagt aaaatgctta tttaagcgag
    20941 ttgtttcgtg tttttctaac ccatgagctc gagcaatcgg tgctaattcg atcacttcga
    21001 tcacttttgc agaagtctct tccatttctt gcctgaagtc tgagttggac tgtctgatcc
    21061 ttttacggaa aaagagagta acgaatgcgg cgagaggaac agtcaataag aaaaacatga
    21121 aaacaacagg acttttataa agcgtgatac ctaaagctac gaataaattg acagcgatat
    21181 ttaatccgct gacaaatact tgagaggaca acgtttggat agcttctaca tcacggatga
    21241 tctttgactg gattcgtcca gattgcaatt ctttttgata aggaatagat agttcttgga
    21301 ttttcttgat aagggtcgct cttagtccag cttctacttg acggatcgaa gtgctatggt
    21361 attttacatg aaaataattc gtcaatatat tttgaccaat cagacaagcc atcaaaaaag
    21421 cgttgaaata aatggcctgc aactgagaag aatctttcga tgaagctgca ttgattacat
    21481 tggcaatagc gatcggcatt aaccaagatg gaagatgttt gataataaaa aagagtcctg
    21541 acatggcaaa acgccaataa tttccctgat aaagatgcag caatgtctga aaagcatttg
    21601 catgatattt ttgataactt ttctgtattc gttcctccat aatcttctcc ttaacaatct
    21661 tggtaaataa actgcaagtg ttctattgct tcaggaacat aaatctcagg agtagcttca
    21721 agagtggcgt acattagagg acgctcatat ttgatataat gcagtaccgg gcggaaatct
    21781 aaacagccct tgccaacagg taccatgcgg atctttccat tttccatgat atagtctttg
    21841 agatgaagcg ctgtaatata accatgtaaa ttgtccaagg cacctcggat cactttttcc
    21901 tgatcagcat gattttcttt atgcatcaaa ttcgcacaat ctaaaatgat ttttaaattt
    21961 ggtgaagcta cttcatcgat taaacgttta gctaactgat agttatagat tggatgattg
    22021 atccctgctt caacagcaac agttacgcca aaattttctg catgagcgac taattcaatg
    22081 atagaatttc tggcgatctg ataagcttct tcagtaaaat tttcttttgt gtaaccgaca
    22141 gagacactac cggtttccgt agcaatcatc ttggcttgat aggcagagca cagagcaatt
    22201 tgctgtttga aagttgccaa tgcttgtgca cgaacttctt gatttttgct ggataggttg
    22261 atatagcatc ctaaaatgga aagttcgata ttgttttttt ggaagtattc accaaaatag
    22321 gcagcactcc caagtgtcat gtcagttaat ttaagttgtt caggaaatgc tttttgtggt
    22381 gcaaattgga tatgggacaa gtgaagctgt gatacttttt gaacgatttc ttcacgattt
    22441 cgaacatcta aatcgtgggc acggatgcct aaatttaatg gaaacatctc aaactccttt
    22501 ttgtaaataa ttagcaagag gcagacggga ttcttttatc aattcaacaa ttattcgggc
    22561 aactttatca gcgccttctg gactaaagtg ggtattgtct tttagtcctt ctgggtaatt
    22621 tttacaagta tcaggcataa gatgcaggta gaattttttt gtctcttctt ttgagaaggt
    22681 ctggaacaat tcttgtgatc ttgagaacac atctaaaaga ggaatatggt gtttctcagc
    22741 aaatgcacgc atcgcttttg gatagtctcc aagtatatct ggattcaatg ttccgttttc
    22801 taagtaatct cgtcgcgtca cagatgtcag taagaccatt tggacgttcg ctttttttgc
    22861 gcattcaaaa aatatctgca gattttttaa atagctaccg aagggaagcg tacctctttc
    22921 ttcttggatt ttctggtcgt tatgtccaaa ttgtacaaat aagaaatcat tcggctgaaa
    22981 ctgtgaagct agacgggcta accttccttc ctggataaat gatttggtgc tgcgtccatt
    23041 ctttgcttgg ttattgatct ggatctcggg tgaaaaataa gcagagattt tttctcccca
    23101 acctgtctcg ggacgcttgt tttcatcttt ttctgccgca gtagaatcac cggcaatatg
    23161 gatagtaggc atgttatctc ttctttcata aaggtttata ggcaatgaag cagaacagca
    23221 agagaagaag acggagcaa
//
LOCUS       DARBFD010000039        23065 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000039 DARBFD010000000
VERSION     DARBFD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.039"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(111..566)
                     /locus_tag="SCK08_001429"
     CDS             complement(111..566)
                     /locus_tag="SCK08_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201313.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="SCK08_001430"
     CDS             complement(720..1928)
                     /locus_tag="SCK08_001430"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEG1201314.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="SCK08_001431"
     CDS             complement(2040..2372)
                     /locus_tag="SCK08_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1201315.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="SCK08_001432"
     CDS             2520..3395
                     /locus_tag="SCK08_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1201316.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="SCK08_001433"
     CDS             complement(3477..4058)
                     /locus_tag="SCK08_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEG1201317.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="SCK08_001434"
     CDS             complement(4064..5323)
                     /locus_tag="SCK08_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1201318.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="SCK08_001435"
     CDS             complement(5325..6371)
                     /locus_tag="SCK08_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201319.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="SCK08_001436"
     CDS             complement(6606..6881)
                     /locus_tag="SCK08_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEG1201320.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="SCK08_001437"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="SCK08_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEG1201321.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="SCK08_001438"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="SCK08_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEG1201322.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="SCK08_001439"
     CDS             complement(9980..10660)
                     /locus_tag="SCK08_001439"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEG1201323.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="SCK08_001440"
     CDS             complement(10709..11344)
                     /locus_tag="SCK08_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1201324.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="SCK08_001441"
     CDS             complement(11410..12813)
                     /locus_tag="SCK08_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEG1201325.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="SCK08_001442"
     CDS             complement(12800..13831)
                     /locus_tag="SCK08_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201326.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="SCK08_001443"
     CDS             13862..14083
                     /locus_tag="SCK08_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEG1201327.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="SCK08_001444"
     CDS             complement(14248..14985)
                     /locus_tag="SCK08_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEG1201328.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="SCK08_001445"
     CDS             complement(15138..16478)
                     /locus_tag="SCK08_001445"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEG1201329.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="SCK08_001446"
     CDS             complement(16715..18238)
                     /locus_tag="SCK08_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEG1201330.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="SCK08_001447"
     CDS             complement(18616..19227)
                     /locus_tag="SCK08_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1201331.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="SCK08_001448"
     CDS             complement(19557..20273)
                     /locus_tag="SCK08_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1201332.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="SCK08_001449"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="SCK08_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEG1201333.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21629)
                     /locus_tag="SCK08_001450"
     CDS             complement(20832..21629)
                     /locus_tag="SCK08_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEG1201334.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="SCK08_001451"
     CDS             complement(21599..21970)
                     /locus_tag="SCK08_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1201335.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="SCK08_001452"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="SCK08_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEG1201336.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6468 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacaat tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt ttttcggctc tataatttat gggactgatg aatcagaatt gattcttcag
    23041 ccccattttc attttaggta ttaaa
//
LOCUS       DARBFD010000040        23014 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000040 DARBFD010000000
VERSION     DARBFD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.040"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(19..585)
                     /locus_tag="SCK08_001453"
     CDS             complement(19..585)
                     /locus_tag="SCK08_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1201337.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(613..2115)
                     /gene="eat(A)"
                     /locus_tag="SCK08_001454"
     CDS             complement(613..2115)
                     /gene="eat(A)"
                     /locus_tag="SCK08_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1201338.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2373..3494)
                     /locus_tag="SCK08_001455"
     CDS             complement(2373..3494)
                     /locus_tag="SCK08_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1201339.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3620..4561
                     /locus_tag="SCK08_001456"
     CDS             3620..4561
                     /locus_tag="SCK08_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1201340.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4711..5607)
                     /locus_tag="SCK08_001457"
     CDS             complement(4711..5607)
                     /locus_tag="SCK08_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1201341.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5876..7216
                     /locus_tag="SCK08_001458"
     CDS             5876..7216
                     /locus_tag="SCK08_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1201342.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7434..7877
                     /locus_tag="SCK08_001459"
     CDS             7434..7877
                     /locus_tag="SCK08_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG1201343.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8381..9799)
                     /gene="nhaC"
                     /locus_tag="SCK08_001460"
     CDS             complement(8381..9799)
                     /gene="nhaC"
                     /locus_tag="SCK08_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1201344.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10202..11425)
                     /locus_tag="SCK08_001461"
     CDS             complement(10202..11425)
                     /locus_tag="SCK08_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEG1201345.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11456..12295)
                     /locus_tag="SCK08_001462"
     CDS             complement(11456..12295)
                     /locus_tag="SCK08_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1201346.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12312..12992)
                     /locus_tag="SCK08_001463"
     CDS             complement(12312..12992)
                     /locus_tag="SCK08_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201347.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(12994..14067)
                     /locus_tag="SCK08_001464"
     CDS             complement(12994..14067)
                     /locus_tag="SCK08_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1201348.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14123..14361)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14404..14637)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14714..15385)
                     /locus_tag="SCK08_001465"
     CDS             complement(14714..15385)
                     /locus_tag="SCK08_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1201349.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15472..18150)
                     /gene="mgtA"
                     /locus_tag="SCK08_001466"
     CDS             complement(15472..18150)
                     /gene="mgtA"
                     /locus_tag="SCK08_001466"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEG1201350.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            18809..19246
                     /gene="ndk"
                     /locus_tag="SCK08_001467"
     CDS             18809..19246
                     /gene="ndk"
                     /locus_tag="SCK08_001467"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEG1201351.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19268..20134)
                     /locus_tag="SCK08_001468"
     CDS             complement(19268..20134)
                     /locus_tag="SCK08_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1201352.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20294..22387)
                     /locus_tag="SCK08_001469"
     CDS             complement(20294..22387)
                     /locus_tag="SCK08_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1201353.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         6624 a         4785 c         4066 g         7539 t
ORIGIN      
        1 aataacacaa aaacataatt actcataaga aaccaattct gtattatcta ccatgaatgt
       61 aaagaacccc tcattaggca tcggatgaaa ataagcattg atcactctta tcaaggcatt
      121 atggcaaact aacagataga cattttcttc gtcttcgatg cattctttca ataaaggaac
      181 gatccgtgca tacacatcta agactgactc cccatttggg aatcgtacag cgaacgctaa
      241 acgtgctttt tgaaagttct catctttgct tggcagtcca tcataatccc caaaatccat
      301 ttctaccaat cgttcatcaa cggtcagcgg caagctgaga cgatcagcaa ctgcttgtgc
      361 tgtatctctt gctcgttgaa gaggggaatg gatgattttc gtgattggaa caggcaaatc
      421 tactaccttt tctgctagtt tctgtgcctg catctgtcct ttttcagtca gcggcacatc
      481 tgcccgtccg caaactaatc cttgtgcgtt ccaagttgtt tctccgtgtc tcgttacata
      541 aagcatgtga ttctctcctt cgttggcaaa gttcccctta accatacctt gttgttcttg
      601 agattacaag ggttagattc tctcaagaga aataatctca gtagatactt tatccaggaa
      661 ggtttgatca tgttccacta gtaacatggc aggtttcacg ttcaagatca gttgttctaa
      721 ttgttcttga ttgaagacat ccaaataatt caatggttca tcccatgtat atagttcagc
      781 tggctgtgat aaagatttag ccaattccac ttttttccgt tggcccatac tcatctgctc
      841 gatcttgtta tgaaaaacat ctctttccat cccaagcttt cggaggttgt tcaaaaatgc
      901 ttggtagtct acttggtttt tctctgcaaa ttccgccaac gttcctcgat tgtcttcata
      961 attttggctt gcataactaa tgctcagatg ttttggctgg tatttttctc ctataacctt
     1021 gccgttgaat gcccccagaa gataatggat aatggatgac tttcctgcac cgttaggacc
     1081 tgaaatcgcc acccgctgat gaggctcgat tgtaaaatga attggctcga ataacagatt
     1141 ttcataccct aattgaagat cttctacgct taaaagtcgc ttatggtgag acgcttggct
     1201 attcatcgtc aaagaatcga tatattcgat atcttttaat agtttttctt tctcgctgat
     1261 ctgggtctcc atccgattca cgattgcttt ggatcgtttc atcgtccgtg cagcatcagc
     1321 acccactgct cctttattca ctcgtctaga ttcagtatcg atgaatccga cttgtttctt
     1381 tgttttatct ccttctcggg aacgagacca ttcggctttt tcagctgctg ttttctttag
     1441 cctactgact tcttttttca atttttcatt ttgagccatt tcaaattcat cacgaagttt
     1501 tttctgttct tcatagatag agaaattccc ttgataaagt tccagttgac ttttttcgat
     1561 tgctaaaaca tggtccacta cttcatcgat aaatccccgg tcatggctga ccacgatgaa
     1621 gccttgtttt ttctttttca aataagccgc tacttgtttt ctaccagaga tatccaaatg
     1681 attcgttggt tcatcgatta acgggaaatg agtgtcatcc acaaataaaa gtgccagtag
     1741 gacttttgtc ttctctcccc ccgatagtgt gctgaattct ctccataaga tttcaggatc
     1801 tgtttgcatc aattggagct ctctttcgat ttcccatatc tcaaaatccg taatatcatt
     1861 taacacgtaa taggttaaac gttctttatc ttttgtctgt tgcgggaaat aggcaaattc
     1921 ttgctgatgg attacttgcc cttgataagg tagtttgttt tgtagaatat tcagtaaagt
     1981 tgtctttcct cgaccgtttc gtccgataag tcctagtttc cattgtgtgt caaaatttag
     2041 atttgcttgt tcaaataata atgtgccttg gctgtcgtag ccgaatgtca gattttttat
     2101 ttcgatttta gacattttcc atcacctcgt aaaaaaagac cagtcctatt ttcagaactg
     2161 gctaaagagg atgcaagggt tggatacaca caaaaagacc catacatcga aaaaaccaag
     2221 ccaatcccga aaatctgcac actgcttcct tgtctccaca aggtacagtc tgaatgcagc
     2281 tttcatcaat cgacttaatt tttcaatgca cggatccctc actttgtcct atatttgaaa
     2341 ttacgatagc ataaagaaaa tgattttgtc aactacttcg ggaggatggt gaaaaatctg
     2401 ctaagtgtct gtcctaagat ttttgttttt tctaatgttt gcctgtgttc cattgcttct
     2461 tctaaaccta ttggacccaa atgaatactt aagacaattg ggagaaaatc agaaagtcct
     2521 tctagctctt ggtctctact tccgcaaata gcgattattg gttttcgata tttttttgca
     2581 agcactgcta caccgtatgg aacttttccg tggatcgttt gctgatcgat ccgcccttct
     2641 cctgtaatga tgacatctgc ttgttcgatc tttttttcca atccagtgat ttggctgact
     2701 aaatcgaaac cagaaaccat cttcgctccc aacaaaaaaa gggctccacc aataccgcct
     2761 gctgctccac ttcctggaat atcatctaaa tccacctccc agaaatcttt tatttgctga
     2821 gcaatctttc ctgcttgttt atctaagaaa gctagcgtcg tatctattcc tcctttttgt
     2881 cttccaaatt gccaagcagc tccttgttga cctgtatatg gattggtaac atctgcagcg
     2941 ataatcaatt ttactttaga aacaagaacg tttcttcgat caaaatgaag ttttttagta
     3001 gataataaag gatttccttc tgtgtaccct tctaaagaag cacccaagga ttggagtaat
     3061 cctagaccac catcgctaca cccgcttcct cctaaagtga caataatttg ttctacttct
     3121 tgaagcagtg catctttaat aagttctcct aaggcataac tggaagcaag tcggatagtt
     3181 tgatccgttg gtttatcaag tagatctaac cccaatgctt gtgcagattc gatgactgcg
     3241 gtgttttttc cttgccaagt tgtcaataaa tagctgattt ttcgctgacg gaataagaga
     3301 tccatcccat caatggtacg ccatgttccc ctgagcgcgt aatagattgc ttctgcagta
     3361 ttttctccgc catctgcaat cggcacattg atttttcttc ctttacgatc ttcccagcca
     3421 tttagaactg cctgatttag ttcaaatgaa gaagcacttc ctttaaatga atcaattgcc
     3481 gccaaaatat ccatctgtat ccctcacttt cttcttaagt aagacacttt ttttcatttt
     3541 tttcaaacaa aacaatgcta ttttcttcca tttttctata tactaaaagg gaactcattg
     3601 aataaaggag agaaaaaaca tgaaaatcgc aatagctggg gccggagcaa tgggcagccg
     3661 aatcggattg atgctacatc aaagcggaaa tgaggtcctc ttgatcgatc gttggcctgc
     3721 tcacattgaa gccattcgta caaatgggtt gattgctgac tttaacggta aagaagtggt
     3781 cgcaaaactg ccaatctatt caccagaaga aatcatcgaa agcaacgaac acgtggactt
     3841 gatcgtagca ttaacaaaag ccaatcaatt agatgatatg ttttgttcga tccaatccat
     3901 tattactgac aatacttatg ttttgtgttt actaaatgga ttaggtcatg aagatgtact
     3961 ggaaaaatat gtgccaaaga aaaatatctt atttgggatc acgatgtgga cagctggtct
     4021 agctggtccc ggaaaagtca cattgttagg tgatggagaa atcgaactag agaacttgga
     4081 accagaaggc gaagcattta caaaaaaagt tgtagaagtc tttcaagaag caaatttaaa
     4141 tccgatctat agccataacg ttcgttattc catttggcga aaagcttgtg taaatggaac
     4201 attgaatggt ttatgcacga ttttggattg taatattgct gaactaggcg cgcaaaaagc
     4261 ggcagagagc atggttcgaa caatcgtatc tgaatttgct tctatcgcag caaaagaagg
     4321 aatcatacta gatcaagaag aagtgtacca acatattgct tctacttatg atcctgacaa
     4381 catcgggttg cattacccat ctatgtacca agacctgatc aaaaaccatc gtttgactga
     4441 aatcgactac atcaatggtg ccatttggcg aaaaggacaa aaatatgata ttgctacacc
     4501 atattgtgcc tttttgaccc aattagtcca tgcaaaagaa ggaatccttg gcgcagaata
     4561 aaaggctctc tgatgagaat cagctgaatc agcagctggt tctctttttt taagcccaat
     4621 aaaagatcaa aaagaaaaca caactctcat gatgccataa gaattgtgct tgtttgtttc
     4681 agtacataat ctcttcaaaa tgtaggtttt ttatttgttt tcaacagagg agaggtcata
     4741 ttcgtattgc tcataaatag tggaatcgtt agtgataaat cgttttgccc aattttctgc
     4801 atcataaaat tccaatagct ctgataggat tggcttaaat ggtgtatgca gaaattccgg
     4861 taattcatct aaatcacgtt ctcgaagcaa aaaaatattt tcaggacgat aaaaatgata
     4921 atgagcaatc gcttgattat ccgtcactag tttcttttga aaatacatgg attcaaatgt
     4981 ccgcaaagtc actccttgct gaccctcccg caacagttcc agaatcccgc gcgattcccc
     5041 tgtttttttc aggtactctt cataagtaag atatccaggt tgttcattcc ctcgatgtgg
     5101 aagaatgaaa tagttggtcg taacatctct ttttataaat tcctgcttat agctttctgc
     5161 tcgttctttt ctcccattat tcgttgcacc aaaaaataga tccgtctttg gttcgctaga
     5221 agaaaaattc atatgtttcg aataaaatga actattatgt ttcagcccaa gttcttttgc
     5281 ttccaaaaaa tcaaaatgat aaatctcatc gattgccggt tcatcttgtg cctgcttcag
     5341 catcgcctcg tgttctgctt tgaagtgatt ccaaaaaaat aacacaattt ttgcattggt
     5401 aaatgggcgc acgcgtcgag ccatctccat atcaaatcca taatctaaaa aaacgatcag
     5461 ctgataattt gccaactgtt gtctagctga tttaatgaat tccgactgta gtaaatcttt
     5521 tccggaccaa acatctaaag agcggatgga aggcaaaaaa taaagtgatt tattttttgc
     5581 aactagcagt acgtcatttt tgttcatctt ttccgcctca ctttctctgt cactttttcc
     5641 tatgatagcc tgtttaaata gtaaaaagcc agtaaaacgt ttcctatatt tgtatgaaaa
     5701 tttaacattt gtatgaaaat atgatgaaat aaatcattct tctattaaaa ttcgcctaaa
     5761 agttttctga atagttgtaa ttgtctgaaa atttgttatc attagtcatt gttaaatatt
     5821 gctatttatt cttaatataa attttgattg tgaatttaaa agaaagaggt catttatgga
     5881 agataaaaaa ttgaaaaaac aacttagctc cagacatatc accatgttag ctttaggtgg
     5941 agcaatcggt gcaggtctat ttaaaggaag tggcgaagcg attgggatcg ccggcccttc
     6001 tgtattgatt gcttttttga ttggtgggct gattttatat atcgtgatga aaggtctggg
     6061 aaaaatcgtt ttaagcggtg gcgacacgca tcacggatta tctggtttga tacgccctta
     6121 tctaggtgct cattctgctg attttactga ttgggtctac tggtctatgt ggattatcaa
     6181 tatcatcgct gaagctgttg cagctgcttc ctttttgcag ttgtggttcc cgcacattcc
     6241 agcctgggta tttgttttta tgttggctgt tttaactacg atcatcaatc tttattcggt
     6301 acggttattc gcagaaacag aatattggct ggcttttgct aaaatcagtg tcattatctt
     6361 actgatcatt tttgctactt atcttgtcgg tcagcaaatg ttgggcacag gcgttttccc
     6421 tacccttcag caattgactg accatggtgg gtttactcct catggtatga agggaatcat
     6481 cagttctttg cttgtagtta tctattctta cggcggttct gaactgattg ctatcacggt
     6541 aagcgaagca gatgatccta aacgtgcgat tcctaaagca atcaaaggtg tcatgggaag
     6601 aatcatttcc ttttacatca ttcctttgtt tttgcttttg attctttttc cttggaatac
     6661 tttagcaggc acgaatgtca gtccttttgt gatggtattt gaaaaaatga atatcccatt
     6721 tgcagcggat atcgtcaatt ttgtgattgt ccttgctttg ttctcatcta tcaattcggg
     6781 tgtgtatgca tcttcaagaa ttttgttctt ccgtttgaag gatcgaaaag gttcctctag
     6841 aaaactagct gttttaaaca agcatcaagt accgcaacgt gctgttttat tttgtaccag
     6901 tgttctttat cttggtgtag ctctttctta ttttgtcggt gacaaattat ttggctatct
     6961 ggcaggttcc ctttcgtata cagtattatt gatctggatc attatcagtg cggcatcctt
     7021 tgtactggca ctaagaaaag gaacattatg ggataaagga atcagctttt ttgcattagc
     7081 tgtcttagga ttgattttcc ttggtatcct gttcacgaac tccattgggg taactgtatt
     7141 gactggcctg ctttacctgt tcatttattt cagttatcaa aaaaagaatg atgcgttcgt
     7201 attgacgaat gaatgaaatc gatagaaaat tttattttat ttatgtggaa tagatggagc
     7261 tattgaagaa aggagttgtg acaaaagtct tgttgcaact cctttttccg aataaacagt
     7321 ctcatgacct cttggtaaac ggtgttcaaa aaccctctgt gcaaaataat tgattgttct
     7381 ccatgctcat gacatgatag aagaaaggaa tgaaaaaaag gagtttgttt taaatgaaaa
     7441 agtattggaa gtttttgttg atgatcggtg tttcaacggt agttatgttt ggtatgatgt
     7501 acttgaatgt ctatgcgctc gatcatatct tcttcagtcg tacacgagta ttcatggcgt
     7561 tgatgatggg tgcagtcatg gcagtcatca tgctgttgtt catgtggaaa atgtatgaaa
     7621 acaaaaagat aaatgttatg atcttagccg ctagtcttct tgtttttgcc ggttccttgt
     7681 ttatggtacg aagccaaact actgtcaacg acacagcttg gatgaaggcc atgattcctc
     7741 atcattctat cgctatcctg gcaagtaaac gagctgattt aaaagatcct gaagtaaaaa
     7801 aattagcgga agaaatcatt caggcacaag aagaagagat cgacaaaatg aaaaaaatga
     7861 tcaaagaaat ggaatgacca ttatccgtcc aaataaaaaa agaggatgtt ccagaagtta
     7921 tcagcttcaa gaaataagaa gaagctactt ctggaacgcc gtgaataagg ggttgtgaaa
     7981 aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagcttctc ctatttttcg
     8041 tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttatta agaggttgta
     8101 aaatcagcgt tctgacctga gtattagaga gacaaatgga aaaatagttc ctcatatttt
     8161 gccatttttt gagctaatac cgagggtcag ctgattgaac accgtttatc gagaggttgt
     8221 gaaaaagtcg cttagtttcg cgtattaaga aaaattttgt gaaatggctt tccacatttt
     8281 tgtataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttat tcgtaaaagg
     8341 gattgtgaca aaagtcttgt cacaatccct ttttatgttt ttattgttct aattgcgctg
     8401 tcttgcttac tgcttcttgc ggtttcacaa atttttttga gattccccaa cctgtgaagg
     8461 cactgaccat cgaaaacaca ggagaaagca aactgaaaaa tacgaatggc aagtaagtga
     8521 gtgtaggaat accaaatgta cttgctacga aacttccggc aattccccaa ggcactaagt
     8581 agttgattac tgttccacca tcttctagcg ttctacctaa tacagttgga tcaaatcctg
     8641 ctttttcata agtttcttta aatgcattac caggcagaat aacagatagg aattgttcac
     8701 caataaaaat atttactccg atacttgtca tcaatgttgt aaaaatcaaa tttggtgtgt
     8761 tcgtcatctt tcttgatact cgttccatca cggttgagat caagccaaat tcgatcaaca
     8821 atccacctaa tgacaaagtt agaataatca atgacacagt aggcatcata ctttcgattc
     8881 ctccgcggct aagcagtgaa tcgatttgtt ggtttccagt atttgaaaca aaccctgatt
     8941 cgatgacccc agccaacttc gttaaggaag tttgtgggtt ttgaataaaa atcattccta
     9001 ctgctacagc gatattcaac aaaatcgtta aaattgccgg cacctttttc catgcacaca
     9061 gtaacattaa aaataaagga atcagtgacc agattgaaat agtaaagttt gcttctagaa
     9121 catcagtcac ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt cctaaaatca
     9181 tgaataaaaa gaaagaaaca acaaatgccg ggacagttga ccacatcata tgcttgatgt
     9241 gtttgaacaa atcggcatca acaatagctg ctgctagatt tgttgattcg gataatggag
     9301 acatcttatc tccaaaaacg gcacctgaaa caatagctcc aacaaccagc gctggattta
     9361 agcctagtgt tgtcccaata ccgaaaaatg caatcccaat cgttgacata acagtaaagg
     9421 cactccctac agcgctacct acgattgcac acacaacaaa tacagaaggg acaaaccact
     9481 ggacactgat caatttgaac ccaatgacca ttagagttgg aatgatccct gcttgtatcc
     9541 atacagcaat caatgaaccg actaaaataa aaataaagat tgggatgatt cctggtttga
     9601 tgccattgat gattccttcg tgcagccaat ctgtcgggta tctttttatc actgcataca
     9661 acatgaccat tccgatcgct gctaagatcg tgatattagg tgagaccttt aatccgacaa
     9721 cgccaatcgc aatgcctaaa acaataacca ttaatacaag tgttgcttct aaaaaacttg
     9781 gtgttccttt ttttcccatg ataatctctc cttttttagt tgttcttatg ttctaaaaaa
     9841 gttaagatat cggattactc cgccgcaatt catccgcgat tcctccttta taaaaataaa
     9901 aagacctgtt ttaaactaag gacgaatgac tccgcggtac caccttaatt aaaaacagat
     9961 ctgtttttca ctttcaaact tttattcaaa tgtatggact ttccatgtaa ttcgtcaatt
    10021 aggcttgatc ggttcacagc gaccaccgac tttctgaaca aaacacctaa ccgctactgc
    10081 gtgaaaagtt gacttgtacg aagtttacct tccataagat catttgtcaa tgaaaaagaa
    10141 aatatttttt catttttctg aaaattattt ttataacttt cacacacaag tttatttttt
    10201 actagaaagt agcgccctct tgtttttttc ttgctactat ttcttgatgt tcttgcttga
    10261 ttctttctaa tagctttggc tctttcaaca gatcaagagc tgtattggcg agtagttctg
    10321 ctgcaatcgc aatagaagca aggcctttgt cacttttagc agcagctttg aattcttctg
    10381 tgtgtccggc aatatatgtc tcagagattg ctaccgtagg ctggattgtt ggtatgactt
    10441 ggctgacatt tcctacatca gaagaaccga gacttgttct ttgttccgtt tctatttctt
    10501 catctggaac acctgcttct tttgcatgct ggaagaataa gtcatcaaaa gaaggagtaa
    10561 cgatgacatc atcaactgca ttttggaaca atccaaactc aaaagtcgtg cccgtactta
    10621 tcgcagcacc tcgtacgata ttttccactt tttcatatac tttatctaat gtttgccgat
    10681 ttcctgcacg taaatagaaa cgtccagcag cgtaatctgg aatgacgttg gcagctactc
    10741 ctccgtctgt aatgatccca tggatattga catcttttgg caattgcaag cgtagagcgt
    10801 tgattccatt aaacacttgg atcaatgctt ccaaagcatt tactcctttt tcaggtgctg
    10861 ctgctgcatg ggaagcgaca ccgtaaaatt tgatgtcgac tggatcattc gctaaagatt
    10921 ctgtcgtttt cccataacga aaagctggat ggacgcataa cgctacatcg acatcctcaa
    10981 aaaaaccttc tcgaacaaag ctgcctttag ctgatccgtt ctcgccccct tcttcccctg
    11041 gggtgccata tacacggatt tctccaccag tttcatcaat gaattgcttc actatgctgg
    11101 cggctaatac ggagtaagta ccaaacaaat tatgcccgca tgcatgacca atacctggta
    11161 aagcatcgaa ttctgccaaa aaggcaattg tgggaccggg tttattggaa atataacgtg
    11221 catcaaaggc tgttcggtgt ccagctactt ctttttctac ccaaaacctt tctttttcta
    11281 attgtttaat caacgctgct gaggaataaa actcatagtt acttacttca ggatgttcgt
    11341 ggatatctag agcaagtgct tgatattctg gtaaacgata ttggattaga tccttcaatt
    11401 ggtttgttga gattgttgtt gtcataaaaa attcctccta tgctagtcat ttttcttatt
    11461 ttctcccgaa tgtttcccat gcaggcacgc ttgatccttt tgatgttttc tcgattactt
    11521 tgactgtgtc ttctgtttga taagcgtcta ctattttttg atacgtttta ttgttttcat
    11581 cttcttttct tgcaacgatg atattcacat aaggacgtgc atggtcatcc actggttcta
    11641 agaagatggc atcagaagca ggattaaatc cagcatctac agctacaccg ctattgatca
    11701 ctgaaatgtc tacatctgac aaagcacgtg ctgtttggga agcatctaat tcagaaatgt
    11761 ctagattttt tctattctcg gtaatatcag aaacagtggg cgcttgtcct tttgccttat
    11821 ccactttgat caatccagca gtttgcaata acagaagagc tctgccgcca tttgtaacat
    11881 cattagggat tgctactttt gctccgtctt tgatatcagc tacatccttc aaagtgttag
    11941 aataaattcc cagcggtgca ttgacagtat ttccgatcga tactaaatca gttccgtgtt
    12001 ctttattgta attgtctaaa aagaattgat gttggaaaga attcaaatca atcgatccat
    12061 ctgctaaagc cacatttggt tgattgtaat ctgtaaattt cacgtattcc aaattaatgt
    12121 tctccttttt caaacggtct ttcacgttgt cccatacatc tgtatcttct ccaataatcc
    12181 ctagttttac gatagtttct tttttgtcag ttgtatctgc ttttccttgg ctacaccctg
    12241 ccaaccaaat aattgctaca aatgtaatga ctacaaaacg aataattttt ttcatttttt
    12301 ctcctccact ttcaatgtgt aattttttta atcagttgat tactgataaa ttgacttaaa
    12361 aaaactaaga ttagaatcaa taatgtagcc acaatcgtta catctgtttg gaatcgatta
    12421 taccctctcg atatagcaag attccctaat ccgcctgcac ctactgctcc agccatggta
    12481 gtcagtccga tcacgttgat gatagtcaac gccgagaccc gaatgatacc aggcaaacct
    12541 tctaacaaat acacacgtaa aatgataccg attggactgg ttcccatcgc ttctgccgct
    12601 tcgatcaccc caggatcaac ttctagcagt gcgttctcga tttgacgagc aaaaaacgga
    12661 acaactccaa aaatcaatgg cactaaagct gctttcgcac caatcgtcgt tccaactaag
    12721 aaacgagtag tcaatgctaa aagagaaagc aagataatga aagggatgga acgaatgata
    12781 ttgatcagct tatcaagtat ttcaaatagc agacggttag ctagtaatcc attagggcgt
    12841 gtcactacta acgtcacacc taagatgatt ccaatcatcc cgccaatgaa tgctgtccaa
    12901 aaagacatat atagtgtttg cttcgtcgct tcgacaaatt catccggaat caaataaaca
    12961 ttgggcaaat atgttttaaa gaaatcagtc attttatctt cctccttcga tcactttgat
    13021 acgactatct aaatcgtttt tttgtaaaat atttattttt acaccagatt tgatcaaata
    13081 atccaatgct tttttccgct gtttggtttc tccagctaaa gtaacgatca aactccctaa
    13141 cggcgtttct tgtaataact ctacattgcc atataaaata ttcgctgtta cttgatattt
    13201 gctgtacaga tgagcgatta gaggttcgtt cgtctgattt ccaatataac ttaattccac
    13261 cagccattta ttggcgatct gatcggcaaa agcagaatgg gcaatgatcg tttctaatgc
    13321 ttggtcaaga tgagtagctg ttcgaataaa atctttggtc aattcttctt caggattgct
    13381 gaagatctgg atgctgctac ctttttcaac gattcttcca tcttccataa ccgctacctt
    13441 gttgcagatt tctttgacaa cttgcatctc atgagtgatc aggacgattg ttaaacctaa
    13501 ctggcgattc aattttttta acaatgcaag gatctgaagg gtcgttttcg gatcaagtgc
    13561 actggttgct tcatcacata ataatacctt aggttcattc gccaaagctc gtgcgattgc
    13621 tactcgttgt ttttgtcccc ccgacagttg tttaggataa gcagatgctt tttcttccaa
    13681 accgacaagt tccagtagtt cattcacttt ttgacgacgt tcctgtttcg attttccaga
    13741 atactttaaa gaaaaatcaa cgttgtcaaa aatagttcgg ctttccatta gattgaaatg
    13801 ctgaaagatc ataccgatgg cctttctttc ttttcgcaat tctttgggag agagctgact
    13861 cagattcgtc tgattgacaa tgacttctcc tgctgttggt ttctgcagca aattcatcac
    13921 acgaactaac gtacttttcc ctgcacctga gtaaccgacg attccataaa catcgccttt
    13981 ttcaatcgtt aaatctacag aatctactgc ctgtatctct ttttcttttt gtttgaaaca
    14041 tacagaaaca tctcgcaatt caatcattaa aattcctcct tatctaaaaa aagcgtcctc
    14101 aagaaaatga aaatgtcatt ttcttgagga cgcttttttg cgtgttacca cctcatgttc
    14161 aagctgtgtt cacacacaac tcctcttcaa gtacgatcat actctagcaa gatatcggtc
    14221 gcagccgtca tcgccttaca tctatgctcg ggatgaattg ctcgatggcc attttccaat
    14281 agtcgttcct tgcttcttct cagctaccga agctctctgt caaaggttct tctatcgtac
    14341 ttaccatttc aatgcaaata atattcaaaa aaaaagtcct tttactaaca taaactatgt
    14401 cagtaaaagg acgacgtatc gcggtaccac cttttttcgg aagtttcctt ccctcaagcc
    14461 caacatctca aaaagagaga ttggcgttcg ttttaacggt cgaacagttc cgaatttgcc
    14521 tacttatcga caaattattc tccaaggcca ttttcagctt ttcttacttg actcttctca
    14581 ccagccagag cctctctgca aagtttccaa acttactttc cttttcaacg aaatcgatat
    14641 tttgttgttg ggattaatct acatgaggaa aactgaaaag tcaagtacga aattcaaatt
    14701 attttttcta tatttatttt tctgtttttt tatttaacat ttcaataaca gttcgaagat
    14761 cttttccttc cgcaagaagt ttcaattttt ccttttcttc gacggtataa tttcggacaa
    14821 aactggaatc aggtatcaaa acaacgccta atcctgctcg tgaagcagct gttgctccta
    14881 ctaaagaatc ttccactgca aaggctgaac tagcttctaa tcctgttcgt tccaaagctg
    14941 tcagataagg aatcggtgaa ggcttgttct ccgacacttc gtctccgaat gtaaacgtgt
    15001 ccaagtaatc agctaattta aagtgatcca agatatctgt tgctcttttc gtaacagtgg
    15061 tcgtagcaat gcctagtttc atatgacgtt cttttaagtc tgccaatatt tcttttgcat
    15121 atggttttaa gtggatggct ccgcttgtca gctgttgata aaaatattct tcacgcttcg
    15181 ctcggacttc ctgtactttt tcagggctac ctgctgcttt cactaaaata tcaaaacttt
    15241 gtttccagtt ttttccgccc caagcgtcaa cgatttcttt tgggatatgg atatcaaatt
    15301 gctgaaatcc ccaaagccag ccatcacgat acgttttttc tgtgtcaact aacaaaccat
    15361 ctaaatcaaa tattgcacct ttcatctata cattaatcct ttccaaaaca atacacatag
    15421 tttagcacga ctggtccaaa aagaacagaa gcaaagaacc agccttctcg tttataagag
    15481 ttcttggaat cgacggatat agattttctt cacagctgtc acaagcgcca gataagcgat
    15541 gactgtagca aataaccata accagtaatt gccaggaagt ggcatcaagc ctaattcagc
    15601 accaaaactt gtaaaaggaa ggacagaacc gacaataatt cctaatgtag tgataacaaa
    15661 catggcaaat gttgcgttgc tttggataaa tggtactttc ggtgtacgca gtgcatggag
    15721 cactaaggtt tgtgtccata gtgattcgac aaaccatcct gcatggaata atgcgatgaa
    15781 aatcactcgc tgtgaactat ccaatgtatg gaaacttccg cccactacag caggacaaat
    15841 aacaaaatac atcaacaagt aagtagtaat atcaaatatc gaacttgttg gaccaaagta
    15901 aaccatgaat tccccaatac tagatgcatt ccattttttt ggctcgtcca aatattcttt
    15961 atccatccga tcccatggca aggaaatgca ggaaacatca tagatcagat tcaaaaacaa
    16021 aagttgtaaa ggcagcattg gtaaaaatgg caggaacgta cttgcaatca gcacggaaaa
    16081 catattgcca aaattagaac ttgctgtagc cttgatatat ttcatgatat tcccaaaagt
    16141 ttctcgtcca gaaagaaggc ctctttccaa aatcgtcaaa tctttttcta aaaggatcac
    16201 gtctgcagat tctttcgcaa tatccacagc tgtatctaca gaaataccga tatcagccac
    16261 tttcattgca ggagcgtcat tgataccatc tcctaaaaat ccgacagtat gaccattttt
    16321 acgtaaagct gtcgtcaaac gtgctttttg ctgaggattc aattttacga atacttcatg
    16381 gtcttcagct acttgtgcta attcatgatc actcatttcc gagattttct caccggtaac
    16441 caattcattg atctctagac ctacttgacg gcacactgaa cgtgtaacta attcgttgtc
    16501 cccagtcaga acttttacag ctacaccatg ttctttcaga gctttcaacg cttgtttcgt
    16561 tgtttctttt ggtggatcaa ggaaagcaag ataaccaatc aaaaccatgt ctgactcatc
    16621 tttgactgaa aattctccaa cgacgcttgg attggtcttt tgtgcgacac caatcactcg
    16681 taagccatct tcattaagtg actgtacttt tgcaagtacg gcttcttttc gttcttttgt
    16741 taatggtgta acagtaccgc caagatcaat aaatttagaa atgttcagca tttcttcaat
    16801 cgcgcctttt gtgatcattt gtgttttacc agctggatca gcaaccacaa cactcatacg
    16861 acgacgttca aaatcaaaag gaatttcatc cactttttga taattgatct tatctgtatc
    16921 taaagtttct gttgcttcat caatgatagc cacatctaac aaatttttca gccctgtttg
    16981 atagtagcta tttagatacg catggcgcaa aacacgatca tcttcatttc cttcacaatc
    17041 catatgatat tcaagaatga ttttatcttg tgtcaatgtc cccgttttat ccgtacataa
    17101 cacatcgatt gcaccaaagt tctggatggc attcaaattt ttgatcactg ttcctttttt
    17161 ggccatcaca cttgctcctt tgaccaaatt cgtcgtaacg atcattggaa gcatttcagg
    17221 tgttagacca actgctacag atagtccaaa aagaaaagct tctagccaat ccccttttgt
    17281 aagcccattg atgacgataa ctgctggagc catcaatgac ataaatttga tcagtagcat
    17341 ggatgtttta tggatgccta attcaaagct tgattgaatt ggtttttcag atagcgtttc
    17401 tgctacatga ccaaatagtg tttcatttcc aacagaaaca atgattcctt ccgcacttcc
    17461 actgatgaca ttacttccca taaagacaag attttcgtaa cttgtttcgc tttgctgatc
    17521 gtcttcacat actttcgcat gcttttctac tggatagctt tcccctgtca atgctgcttg
    17581 ggaaatgaat aaatcctttg cggacaataa acgcatatcc gctggtatca tatcccctgc
    17641 tgaaagccga acaagatctc cacagacgat ttcttccata ggaatctctg tgtactttcc
    17701 attacgtttt acagctgccg ttactttcac aagtgatttc agtttttcag ccgcttgatt
    17761 ggaacgtacg gactgaatca acgtcattgt cccgctgacg ataaccatag tgaagataat
    17821 ggctacaccg aaaagatctt tatctccagg agctgctaat acatattccg tgatgaatga
    17881 aataattcct aatgcgatca acactaatgt aaatggcgtg atgtatgctt tcaatatttc
    17941 tacaatgaat ggtgtttttt tgccatatga aatcgcattt tcaccgtaat tttctcttga
    18001 acgttcgatt tgttcttctg tcaatccatt tgctgatgca tggaatagtt caaataattc
    18061 ttgtttcgtt tttccagcaa aagctgcata gattttttgt gtatctttct ttttttccat
    18121 ctcaatctct cctttgtgtc caaaagacag ctgttctcgc tgcttttggt ctttagctat
    18181 tcatcggatc gatagagaaa aaactgaaag gggctgagaa cagatagata atagccctgg
    18241 cctttcctct agatccttgt ttttcgaatg actgtcatcc atttgttctc atctccttta
    18301 ctaagtaatt ttaattcatg acttccaatc atttttcgaa tagttatttt tccaattaga
    18361 ccacctcctt tttatttttc accgatgggg atttgcggca acaaaaaaac ctctcgccaa
    18421 atactcagcg aaaggtttga aaaagcatat taaaaaaagc ttctcagatc tcatcgttga
    18481 gttttgacac tgtacaacgt aaaaagcaga tacctgcttt tagctacctt atgaaaagcc
    18541 ttatctcgac aattcctgtt ctacccattg gcgtctctcg acgtttttgg gcagtagcct
    18601 atgtttgtat aggagcctca cctaacaaat cttattcggt tttccttcgt taatataaca
    18661 actaaaaaag aggatgtcaa atcaattttc catgtttttc atactttttt taaaatacac
    18721 acgttatatt cctgtctcac gcggttttat ttgtctcatt ttccacctcc cgctataata
    18781 gaaatatagt aggaaacgag gtgctgttat ggaaagaaca cttgttatta tcaagccaga
    18841 cggcgtgaga cgccatttag tcggaagcat catccaacgt tttgaagcaa agggactggc
    18901 cattgctgaa atgaagtttg aaactatgac tcctgaatta gcaaaagaac attatcagca
    18961 cttgactgag cgcagttttt ttgatgaact gatcgattac atgacttccg gtcctgtcgt
    19021 ttatcttgtg ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg ttggagcaac
    19081 aaaagctgcc gatgctgttc caggtacgat ccgaggagac tacgcgctgc cggggactga
    19141 aaacatcatt cacgcttctg attcccgtga tgctgcggta aaagagatcg cccgcttttt
    19201 cccgccattc gatataaaca aaccgtctga aaaagcggcg aagtgataca aattctaaac
    19261 tgctggtcta gttgctgggt tcagcaatgg ccagcagttt tttctctaag gacctccgtt
    19321 gattctttcc taacatatat tcgagcactg tgaagaattc ttggtagtct ttcgtctttt
    19381 cttcccctaa agcagcagaa atcaatcttt gataataatc tgccatcaat gtttcataga
    19441 tcactgtact tctttcttta tgtgctaagc tccgtttcat atcttcattc acactacgtg
    19501 cctttttcaa ccgaccattt tctatataaa acgaagtcag gttcattaaa aaagtaaaga
    19561 gatactgctc atgattgttc aactgtgcta atcgtcgcat tctttttttg attttcaaat
    19621 aattgttctc aatgaaccga tcactgaata tccaaggaaa atcagctaga atagatagtt
    19681 caaattgacc atagtctggt atctgcatca agtattcttc aatgattcgg cattcataac
    19741 gtaaaaattc aaattctgtc tcagatactt ctgttttttc gaccatgtga agattcaaaa
    19801 ccaaataata cagatcttct gttttcttaa aatgcctacg gttattgctg atctctttat
    19861 aaggatcaaa gctgtcttct tgtacccgat ctagcattcg tctttttttc tttaacttta
    19921 ttggttcatc cagctggttt cgcatccatt catattctgc aagtgtcatg ttcattcgct
    19981 ctaaaaatga tctcaatgtt acaaatgaca tttttttcac tgaatttgtc tcaatcccga
    20041 ctagagaagt cttaaaacag attccttccg ctaattggga ctgtgtcaac ttcttgttga
    20101 ctcgaagttt cttgatcaat tcaccatcat acataatagt tctccttttt aatttaaatt
    20161 tttccacatt caataaatag atacgttaaa aaggaaaaat acacgaaaaa aataaaaaaa
    20221 tgtttttttc ataaaaaata ccaaccttca agcattagaa tgttgtctaa ctactgaagg
    20281 ttggtttgat tgtttattga ttattttttt gctgttcaac gtctgttctt gttactggat
    20341 ataagttggt tgttgtttta ttaccatttt ttgaaaagac acttgtcgaa tgttcgcctt
    20401 tttgcttttc gattgcttca ttacgttccg cttcattttt atagtcatat tttttcggat
    20461 caatcgtctc aaatccgttt ggcacataaa accgcaagag attttcctga ttcagcttat
    20521 ctgaaagact taacgcagtc ttgacttttt cctgatcttt ggagatttcc tcatcgattc
    20581 ctgctgcttt cgtatcgatg atttctcctg tctgattatt gtaagctttt cctcctagga
    20641 cggtgtattt gggtgtcaca aagttcccat ttcgaaaagc aactacttga tcatgctgag
    20701 aagataataa gtctgtacca aaatgaagat aatctttatc atcgattccc aatagatgta
    20761 gtaatgtcgg caaaacatcg atttctccac cgtattcatg attgactgtc cctttagtat
    20821 aaccaggcat atggatcatc aatggcactc gttgcatctg tgcgttgtca aattcatccc
    20881 atgtatctgg atctttacca agagcgcttg ctaaatcttt attatctgta ttcgagatcc
    20941 caaaatgatc cccgtaaatc acaaagattg aattttggta aataccagat tttttcaaat
    21001 acgtaaaaaa ttgttccaat gattgatcca gataatgtgc cgttcgaaca taattatcta
    21061 ctgtgctatt gccagtattc aaagatggaa agtcgaaatt cttgtcatcc gtataataag
    21121 gcgtgtggtt ggttacggaa agaaatttcg tgtaaaacgg ctgttgtaga tgctcaagat
    21181 attgggcaga ttctctgaat aaatctttat ctaatatccc ataacctaac gtttcgtcgg
    21241 attcatcaaa ataagatcga tcaaagaaat tgtcataacc taagttttta tatacatgat
    21301 ctctattcca aaaactagct acattcccat ggaaaacagc gcttgtatag ccttgctttt
    21361 gtccaaggat cgctggtgcg gcttgaaaga cattgtctga cccaagttct gtgaataatg
    21421 agccttgagg taaaccaaat gtgccagttt ccaacatatt ttcagcatca cttgtctttc
    21481 cctgtcctac ttcatgaaag aaattgtcaa agctgatcgt tgcttgattc tggaaaatac
    21541 tatttaaaaa cggtgtgact tcttgtccgt ctaccttcat attaatcaaa aactgctgga
    21601 aactttctaa atggagaacg atcacatttt tccctttagc gatcccaaat gtttcgggat
    21661 ttggctctgc ataatgtttc tttgtataat ctaaaacttc atcaatccca ttactgcttg
    21721 catgcgcgcg aacactgcta gtcattcctg ttttgattcc gtcataaata gtatacgcat
    21781 cgattcctag atatttgaca atatatgaac ggtcaaatgt tcgctgcagc aattgtggac
    21841 ggttggcttc gctcagcgct agatttcctg aaaaaataag ccctgaaaga caggtaacag
    21901 cgatagccat cggtttgccg acagggttgc ttttgatagg tattttcttg accttcaacc
    21961 aaaccagtag cccaatgaac acagcgatat ccagccagta gatgacatca tggggcttca
    22021 tcaaagaaaa ggagcttcct gacaatcctt gcgacacttt cgaaaagcct aatacagatt
    22081 tgactgtaat gaaatctgtg aattcccgat aaaaaataat attcaagtac aacaaaagcg
    22141 tatttaaaat atcaatgatc aaaagaatag ctaacgctgg ctttggcttt ttaatataca
    22201 aagaaaggcc aaaaaacaat aaggtagtcg caattgggtt gatccacaag ataaagtact
    22261 ggatcgtacc ttccacacct aatgaaaaat ctgcatagta ggcaatgatc gtcttcagcc
    22321 agaaaaagaa aacagctgtc aaaacgattg ctaaacgatt ttggcacaat tttttaagtt
    22381 gtgtcacgta tatcacttct ctctattcac attttttctt attttgctcc tgaaaaatga
    22441 aaatttccta aaaaagttgt tatcttaaga tttttttatt ctttctaaag attattccgt
    22501 ccacttttca cagcataaaa ccgctgcgat gtaagcgatt aaactaatga agagataaaa
    22561 gtcttgtggt gaccgcttca ctccacggta ttcaaaagcc agtttctcgg tattcgtaca
    22621 aaaaaagagg ctgtgacaaa attcttgtcg cagctcctta tttcttgatg caggacagct
    22681 ttttcacaac ttcttcctta aacggtgttc tcctgccaac tcctcggcca caagtcacaa
    22741 tatttataaa acatgagaag ctgttttttg aaattgttcc ttgtggctca gagttaaacg
    22801 gcaggctcaa gacctcttga tacacggtgt tcaatcagct gaccctcggt attagattat
    22861 gtcaggacgc taattttaca acctcttggt aaacggtgtt caaaagcttg catctgcggt
    22921 aataagccca accaaacgaa aaatataagg agctattttt tgaaattgct ccttgtggct
    22981 cagagttaaa cggcaggctc aagacctctt gata
//
LOCUS       DARBFD010000041        22805 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000041 DARBFD010000000
VERSION     DARBFD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.041"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            13..237
                     /gene="nrdH"
                     /locus_tag="SCK08_001470"
     CDS             13..237
                     /gene="nrdH"
                     /locus_tag="SCK08_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEG1201354.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            396..2555
                     /gene="nrdE"
                     /locus_tag="SCK08_001471"
     CDS             396..2555
                     /gene="nrdE"
                     /locus_tag="SCK08_001471"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEG1201355.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2605..3570
                     /gene="nrdF"
                     /locus_tag="SCK08_001472"
     CDS             2605..3570
                     /gene="nrdF"
                     /locus_tag="SCK08_001472"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEG1201356.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3659..4798)
                     /locus_tag="SCK08_001473"
     CDS             complement(3659..4798)
                     /locus_tag="SCK08_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1201357.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5107..5820)
                     /locus_tag="SCK08_001474"
     CDS             complement(5107..5820)
                     /locus_tag="SCK08_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEG1201358.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6074..6775)
                     /gene="nagB"
                     /locus_tag="SCK08_001475"
     CDS             complement(6074..6775)
                     /gene="nagB"
                     /locus_tag="SCK08_001475"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEG1201359.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7009..8541
                     /locus_tag="SCK08_001476"
     CDS             7009..8541
                     /locus_tag="SCK08_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1201360.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8732..9934)
                     /locus_tag="SCK08_001477"
     CDS             complement(8732..9934)
                     /locus_tag="SCK08_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1201361.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10016..10705)
                     /locus_tag="SCK08_001478"
     CDS             complement(10016..10705)
                     /locus_tag="SCK08_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1201362.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10811..11179)
                     /gene="rplL"
                     /locus_tag="SCK08_001479"
     CDS             complement(10811..11179)
                     /gene="rplL"
                     /locus_tag="SCK08_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEG1201363.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11232..11735)
                     /locus_tag="SCK08_001480"
     CDS             complement(11232..11735)
                     /locus_tag="SCK08_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEG1201364.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(11939..12066)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(12145..12834)
                     /gene="rplA"
                     /locus_tag="SCK08_001481"
     CDS             complement(12145..12834)
                     /gene="rplA"
                     /locus_tag="SCK08_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEG1201365.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12938..13360)
                     /gene="rplK"
                     /locus_tag="SCK08_001482"
     CDS             complement(12938..13360)
                     /gene="rplK"
                     /locus_tag="SCK08_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEG1201366.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13703..14371
                     /gene="sdaAB"
                     /locus_tag="SCK08_001483"
     CDS             13703..14371
                     /gene="sdaAB"
                     /locus_tag="SCK08_001483"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEG1201367.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14384..15256
                     /gene="sdaAA"
                     /locus_tag="SCK08_001484"
     CDS             14384..15256
                     /gene="sdaAA"
                     /locus_tag="SCK08_001484"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEG1201368.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15347..16366)
                     /locus_tag="SCK08_001485"
     CDS             complement(15347..16366)
                     /locus_tag="SCK08_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1201369.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(16539..17444)
                     /locus_tag="SCK08_001486"
     CDS             complement(16539..17444)
                     /locus_tag="SCK08_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1201370.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(17503..18048)
                     /gene="nusG"
                     /locus_tag="SCK08_001487"
     CDS             complement(17503..18048)
                     /gene="nusG"
                     /locus_tag="SCK08_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEG1201371.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18149..18319)
                     /gene="secE"
                     /locus_tag="SCK08_001488"
     CDS             complement(18149..18319)
                     /gene="secE"
                     /locus_tag="SCK08_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEG1201372.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18343..18495)
                     /gene="rpmG"
                     /locus_tag="SCK08_001489"
     CDS             complement(18343..18495)
                     /gene="rpmG"
                     /locus_tag="SCK08_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1201373.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18657..19301
                     /locus_tag="SCK08_001490"
     CDS             18657..19301
                     /locus_tag="SCK08_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1201374.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19357..19860)
                     /locus_tag="SCK08_001491"
     CDS             complement(19357..19860)
                     /locus_tag="SCK08_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1201375.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(19873..19919)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20457..21422
                     /locus_tag="SCK08_001492"
     CDS             20457..21422
                     /locus_tag="SCK08_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1201376.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21521..22693)
                     /locus_tag="SCK08_001493"
     CDS             complement(21521..22693)
                     /locus_tag="SCK08_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201377.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22680..>22805)
                     /locus_tag="SCK08_001494"
     CDS             complement(22680..>22805)
                     /locus_tag="SCK08_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201378.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         7127 a         4584 c         4099 g         6995 t
ORIGIN      
        1 gaggcactca ccatgaacgt aaaaatcttt tctaaaaata attgtatgca atgtaagatg
       61 gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag aagtcaatat cgatgaacag
      121 cctgaagcag tagactggtt gaaagcacaa ggattccaaa gtgttcctgt cattacttct
      181 gatgcagaaa cagttatcgg attccgtcct gatcagttgc gtaaacttgc cagctaacat
      241 gactaaaaga atcaaaacgt gatacatgga tttgaagggc ttgagacagc accagttttt
      301 gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag
      361 catcatcaaa tacgaaatga aggttgggcg tacgtatgag tcttaaaaat ttacaagatg
      421 tcagttattt caagctgaat aatgaaatca atcggccagt caatggtcaa atccctttga
      481 ataaggataa agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga
      541 cttttctatc tattatggaa aaaatcaatt acttgatcga tcaagaatat atagaagcag
      601 aattcattca attgtattca ccatcattta ttgaaaagct atatcaattt ttggacgagc
      661 aacatttttc tttcaaatct tttatggctg cttacaaatt ttattctcaa tatgcgttga
      721 aaaccaacga cggttccgct tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc
      781 tttactttgc gaacggggat gaagaattag cacttgctct tgcagacgaa atgatccatc
      841 aacgatatca gccagctaca ccttctttct tgaatgctgg acgtaaacgt cggggcgaat
      901 tggtgtcttg tttcttgact caagtcactg acgatatgaa ctctatcggg cgctcaatca
      961 actctgcttt gcaattgtcc cgtattggtg gcggcgtagg gattacttta tcaaatcttc
     1021 gtgaagccgg cgctcctatc aaaggatatg agggtgctgc aagtggtgtc gttccagtca
     1081 tgaaattatt cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt caaggagctg
     1141 gcgtggttta tttgaacgtt ttccatccag acattattgc tttcctttca gcaaaaaaag
     1201 aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata
     1261 aattttatga attgacacgt aaaaacgaag atatgtattt atttagtcca tatagtgtcg
     1321 aaaaagaata tggtgtccca ttctcttatg taaatattac agctgagtat gatcatctag
     1381 ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct
     1441 caaaattaca acaagaatct ggctatcctt acgtaatcaa tattgataca gcgaaccgcg
     1501 agaatccaat cgatggcaag atcatcatga gtaatttatg ttcagagatt ttacaagttc
     1561 aaacgccttc tgtgattaac ggcaaacagg aatatgaagt attaggaacg gatatctcat
     1621 gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg
     1681 tgcgtacgat gactcgtgca ttgacattta ttacagatgc atctgatatc gatgtcgtac
     1741 caccgatcca aaacggaaat aagctgaatc atacgattgg attaggtgcc atgggcttac
     1801 acacttatct tgcaaaagaa caaatggaat atggctcaga ggattcatta gactttacaa
     1861 atatttactt catgctgttg aattattgga cacttgttga aagcaacaat atcgctcgtg
     1921 aaagaaaaca agttttccat aattttgaaa aatcagctta tgctgacggc acttatttcg
     1981 aaaaatacgt aactggagaa ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag
     2041 gcatttttat cccaactgct gaagattgga atgctttgaa acaagcagta atgaaagatg
     2101 gactgtatca tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca tatatcaacg
     2161 atacgagtgc aagtattcat cctatcactc gcatgatcga agaacgccaa gagaagaaaa
     2221 tcggtaaaat ttattaccca gcgccttatc tatccaatga gacgattccg tattacactt
     2281 ctgcttatga tatggacatg cgtaaagtaa tcgatgttta tgcaatggca caacaacacg
     2341 tcgatcaagg catgagtttg actttattca tgcgttctga aatcccagaa ggcatttatg
     2401 aatggaagac aacaacgaag caaacgacac gtgatttgaa tattttaaga cattacgctt
     2461 tccacaaagg aatcaaatcc atttattacg tacgtacctt tactgatgat gcagaagaaa
     2521 tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca tagatagatt cgcttattca
     2581 tattttaatt aaaggagcat tctcatgtca gaaacatatt atgaagcaat aaactggaat
     2641 gaaattgaag atatcattga taaatccact tgggagaagc tgacagagca attttggcta
     2701 gatacacgta tcccactttc aaatgaccta gatgattggc gtacattgtc tcaattggaa
     2761 aaagatacag taggacatgt atttggcggt ttgacactgc tagatactgt ccaatctgaa
     2821 agtggcatgg atcagttgcg taaagatgtc cgcactcccc atgaagaagc cgttttgaac
     2881 aatattcaat tcatggaatc cgttcatgcc aaaagttatt cttctatttt tagtacactg
     2941 aatacgaaaa aagaaatcga agaaattttt gactggacaa atacgaatcc ttatctgcaa
     3001 aaaaaagctg agcgtatcaa tgaaatctac aaacacggta caccgctaga aaagaaaatt
     3061 gccagcgtct ttttagaaac attcttgttc tattcaggtt tttatactcc tctttattat
     3121 ttaggaaaca acaaattggc taacgtagca gagatcatca aactaatcat ccgagatgaa
     3181 tctgtacatg gaacctacat cggttacaaa ttccaattag gattcaacga gttacctgaa
     3241 gcagaacaag aaacgttaaa agactggatg tacaatcttc tttatgagct ttacgaaaac
     3301 gaagaacgtt atacagaaga attatacgat ccaatcggct ggacagaaga agtcaagaca
     3361 ttcctacgct ataatgccaa caaagcattg atgaacctag ggatggatcc actctttcca
     3421 gatactgcca atgatgtcaa cccaatcgta atgaacggga tttcaacagg cacaagcaac
     3481 catgacttct tctcacaagt cggaaacggc tatttgttag gtcatgtaga agcgatgaaa
     3541 gacgacgatt atttgattgg attggaataa ggatttattt taattcaaaa atttttcgaa
     3601 aaataaaaag agtttagcgt acagatattt taaatgtaca ctgaactctt ttttatgctt
     3661 attcacttgt ttcttccagc acttttggac tgtctaactt ttccgaaaca tgttccattc
     3721 ttctcaataa atagattttc cggatattta atattatcgt cttaatcact gcatagacag
     3781 gtatgcaaat cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga
     3841 tgatcgtcaa aggatggata ttcattgatt tcccaaataa caacggcttg atcaaatttc
     3901 catctaattg ttggatcact aaaatcgaca acgccatata caaagcttga aatggagaga
     3961 taaacaaacc gacaatgatt gctggtaccg ctccaataaa agggccgaca tacgggataa
     4021 tattcgtgat tccgcaaatg accgcaagca acaagctata aggctgatcg aatattctca
     4081 ttgccaaaaa gctcattaca ccaataatca aagcatctaa gatcgtactg ctgatatagg
     4141 cagataatgt ttcgtttaat tcttttaccg tttggcgtaa ctctttacga atcgctttcg
     4201 gaaagaagtg agacaacgca tcaataaatt tgtgcccatc cttgaacata aatagcaaaa
     4261 taaatggaac ggtaaataac aaaataaaaa attgcatcat aaatgaaatg atttttgaga
     4321 cacttgcaga taaactgact aatatgatat tgaacaaatt actaagagat aaatttgctg
     4381 atgctaattg ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc aattcattta
     4441 accatttgct tgtttcatct gcatacattg gcaagctctg ggtcaattga attgtctgtt
     4501 ccattaactg aggcactaca ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa
     4561 acgtcaataa aaaaccgaac actctcggga tccgatgctt ttcgagaaaa acaacgattg
     4621 gatcgaacat ataatagata aaaccagcaa caagcaatgg taaaaaaact gccgaaagca
     4681 ttttcaatat tggactaaaa atattcggca tttgtaaaat aaagtacacg ccgatgatcg
     4741 tgagtactag ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac
     4801 atctcctcca aggtcctttg atacttctat tctatcattt aacaaagagt tacgcgctga
     4861 gattttcatt tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag tagacgagta
     4921 cttaaacaaa aacacggttt ggagtagtga caaaaattat actgcagtcc ctttttccaa
     4981 ataaacggtg ttcaaaagct tgcatcttcg taataagccc agccaaacga aaaatataag
     5041 gaactatttt tatttggctg ggcttattac ttgatgtagg acatctattt tacaacctca
     5101 gatcatttat tgcgcaatac tgactaaatg caactttgtg atatttttgt gtacggacaa
     5161 ctcctcgatc acatcggcat aagtgagccc ttttggaaga tcaatcgtat agatattttt
     5221 gtacactcgg tagtcatcaa cgaattttac gtcaaaattc acatcttcga ttacgatatt
     5281 tttctcttca aaataatcgg taagaaaatc tttggtttct tttctatgga taaattggat
     5341 ttctaacttt ttagtcgtag gtacatggat cacgcgtttc actactgtga gtgcaatagc
     5401 aatcccgata aaactgatga tcgcaatgga atagtaaccc atgccgatgg cgatccctaa
     5461 tccagcaatt gcccataaag aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac
     5521 gatgatcgtt cccgcgccta agaaaccgac accactcacc acttgagcaa tcagccttgc
     5581 ttcatctgaa cgaatcgttc cagcaagctg tggatactct aaagcatctc ttaatgcacc
     5641 actagcgatc tcaacttgga tcaatgcaat gatacatgct cccatacaaa ctaaaatatg
     5701 cgtacgcata cctgctggac gatttttata ttgtcgttca aaaccgattg ctccgccaaa
     5761 tagcatagcg atccctaaac gtaaaataat ctctggaatt gtcaatacca tatcattcat
     5821 ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt
     5881 tttacctctt acgaaaaaag gactgtgaca aaatttttgt catagtcctt tttatgaata
     5941 aacggtgttc aatcagctga ccctcggtat tactcaacaa acggcaaaat atggggagct
     6001 atttttcctt tgtcttctct aatactcagg tcaaaacgct gatttcacaa cctctttagc
     6061 ctactattga aaattacaac ttacttgctg cttcttcatc gatgatcaca acaacattgt
     6121 cgtgattttg caatacactt gctggcaaat gattcgttac aggtccttca atcatccctt
     6181 tgattgcatc agctttgctt tcgccaaatg ccatcaagac gatttcttta cttttcataa
     6241 ttgaaccgat tcccattgaa acagcttttg ttggtacatc ttctgcttta tcgaagaatc
     6301 gtttgtttgc attgattgtt gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg
     6361 aagcaccagg ttcattgaaa ccgatatgtc cattttgccc gattcctaag atttggatat
     6421 cgattggatg tgcatcgatg atttgatcat actttttgca tgctgcttcc agatcttctg
     6481 cttttccatc tggtacaaac gtttctttga atggtttttt atcaaataat tggacatcca
     6541 tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc aacgtactca tctaagttta
     6601 cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat aaagtaactg
     6661 gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt gttcatacct tctttgatga
     6721 tttcaaatgc tttttttccg ccttcttcag cgttttttac acgaatgatt tccatagtga
     6781 ttgctccttt atattttggt atagcccaat tatagcattc tttttttctt tttacaataa
     6841 ttagtataga ccaaattgca tttttttcat tttcttgaca ataaatagga atccgtgtta
     6901 ggtttcataa gatggttcaa tcaaacaatc catgttgcat gaaaaccaag caagtgattc
     6961 actgtggacg ctttatgtct gcagtttttt tatgaaaaag gagttattat gagtatactg
     7021 acattaaaca acattaccca gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg
     7081 caattaaatg ctggcgaaca tcttgggctg attggacaaa acggagccgg taaaagtaca
     7141 ttgattaaaa tcatcactgg tgagatctta ccagatgacg gacagatcca ttggcaaaaa
     7201 aatattcaca aaggttacct cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa
     7261 gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt
     7321 taccaaacat atagcgagac cttcgatgat tccttgctgg aaaaagctgg gaaactccaa
     7381 acggaattag accaaggagt gttttatcaa atcgacacct tagttgctga gatgagcagc
     7441 gggttgggga tcgatgtctt aggtttagat tcactgttga aaaatctaag tggtggacaa
     7501 cgttcaaagg tgattttagc aaaactactt ttagaaaacc cagatgttct gatcttagat
     7561 gaaccaacca atcacttaga tgatcagcat atccaatggc tgacgcagtt tcttcaggat
     7621 tttgacggtg cgtatatcgt gatttcccat gaccaagaat ttcttgatca gatcaccacc
     7681 catatcgcag acatcgagtt tggaaagatt actaagtata ctggccatct aaaacaagca
     7741 ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa
     7801 caaatcgaaa agaccgaagc ttatattcga aaatacaaag ccggttcccg atcaacgatg
     7861 gcaaaaagcc gacaaaaaca attagataaa atcgaacggc tgacgcctcc agcttcagca
     7921 agtaaagctg tctttgattt tccattctcg cctattgtca cgacattagc agtggaaaca
     7981 actgatttag tcatcggata tgatcgaccg ctacttgcac ccatcaattt gaccatccgc
     8041 tttggtgaaa atattgccat tcgaggcttc aacgggattg gaaaatctac tttattaaaa
     8101 acactgatcg gtgagattga aaaaataagt ggcgatttcc acttcccaga taacacaaaa
     8161 atcaattact tttcacaaga tctgtactgg gaaaatccat tagaaacacc actacaatat
     8221 ttaagcagtc aatttccaaa agcgactatc aaagaattac gtcgccaact agctaaagca
     8281 gggttagtta atcaattagc aagtgaacct ctcactacat taagtggtgg ggaacaaact
     8341 aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt tactgattct cgatgaaccg
     8401 acgaaccata tcgatcaaga aacgaaagaa agcttacaag acgggataca gaaatttcca
     8461 ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc aagatcttgt gcaacgagtc
     8521 attgagattg aaccaaaata aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa
     8581 aagttttgtc acaactcctt cttctgaata aatggtggcc aattagctga ccttcggtat
     8641 tagctcaaaa aacggcaaaa tatgaggagc tatttttatt tggctattct tattacttga
     8701 tgcagaacag cttttcacaa cctcttttcg tctatttttt attttcgaga tttctctttc
     8761 ctttaaaaac aactgtttga aagaagaacg cacatccttg aaaaacaagc ccgctaaccg
     8821 ttacacctaa ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac
     8881 ctccaataaa gtacataaac ataaatacag tattatttcg gttgcttgct gcttcaccta
     8941 gcccttgtac tcgtgtttga ttggcgactt gaccaaattg attcccgacg tctaataaaa
     9001 taatagctaa aagcaaaatg gccaaatggc ttccgccaac caataaagca ataaagctta
     9061 ttgtttgcat aacaagtccc atatacacga tttttctttc agaataagaa tctgccagtc
     9121 gtcccacgat aggtgcagca actgctccag acaaaccaaa aatcgctaat atccctgctt
     9181 cgaatgttcc ccaattgtag gcgggactgc tgatataaaa aatcaacgta gaccaaaaga
     9241 tcgaaaatgt tccaaacatg aaaaatccac tgactgccgc ttctcttaat agacgttgag
     9301 aagcaactaa atgcggcaaa cttttaagtg aatcaaggta tgaaacagct gtactacttt
     9361 gctggacaga acgcggcatt ttccagtaaa gcagaagtgt aagaattccg actgcacata
     9421 aagcaaataa atagatcatc cgccaagaaa aaagactggc aacaatacca gataccgtcc
     9481 gagaaagcaa aatccccacg agtaaaccgc taagcaatgt acccatgacc ttccctcttg
     9541 cttttggtcc cgctagtacc gcaccgtaag gaatgatgat ttgcggcacg atcgaaagta
     9601 atcctattaa aaaagaagcg catgcaaata acgtaaacga aggcgcaaaa aaagcggcca
     9661 acagagagac ggcagataac gctgccattc gaatgatcaa ttttggacga tccaccacgt
     9721 cccctaaagg aactaaaaac agtaacccta acgcatagcc taattgggtc aacatcgtca
     9781 atatcccgac tgcacttgtg ctaacagata aactagctgc tacttttgtt ccaatcggct
     9841 ggatgtaata catattggca acaacaacgc cacaagtgat tgataatagt aaaataagcg
     9901 aagttgttaa aggttttttt tcattactct tcattgattt cccccatttc ctatcttagt
     9961 ttacaactta tggaaaagat tggaaataga aaaatacaac tattcgccac acttattatt
    10021 tgtgcttttg ttttccttgt tttttcttgc gatctttata aatgaagaca actaacccta
    10081 ctagcacagt aacacttgca accaacgtga ctgcagtttt gttatatccg tgaaacagag
    10141 cttctccacc acacgcatac aacacagacg aagggatgac tccaatcacg atcgacaata
    10201 tgatttgctg tagaggcaat ttcatcgcat ctgcagcaaa attgaccata gaagatggaa
    10261 taaccggtat catatagcct aacattacac ctatttttgg atgtttcatt tgtcggattg
    10321 cttgcaccca atgattcgtt tcggttttct tatctaaaaa ttttaagcga tgcataagga
    10381 aaattgcagt gatgttacca agagcattac ccaaaatgtt gatgatcgca cctgtcagtg
    10441 gtccaaagct caaaccagca atgactccaa ctactgaagt aggaatacct ggaacagcac
    10501 acatcaccgc tgtcaatacg atcaagagta tcgatgcaaa agtcccatgc gaacgaatat
    10561 gtgcgagtag ctctgcctta ctcgctctcg gatttaaaaa taattggatg tctgtctgat
    10621 attctaaata caaccgatag cccactacca gtatcagaat gattcccaac gagatcaata
    10681 tcaatcggat tattttcttt ttcatatttc ctcctgaaaa aaatctatat tcattaaata
    10741 atagtttaac ataaaaacaa aaaagcaccg aaatccaaca taggatctca gtgcttttaa
    10801 atagtaaaaa ttatttaaca gttacagatg cgccaacttc ttcaagttta gctttcaatt
    10861 cttcagcttc ttctttagat acgccttctt taactggtgc aggagcgcca tcaactacag
    10921 ctttagcttc tttcaagcct aaaccagttg cttcacgaac tgctttgata actttaactt
    10981 tttggtctcc agctgcagtt aattctacag tgaattctgt ttgttcttca gcagcagcag
    11041 cgccaccagc agcagctaca gctacaggag cagcagcaga tacgccaaat tcttcttcaa
    11101 tagctttaac taggtcgtta agttctagga tagttgcttc ttttaactca gcaacgatgt
    11161 tttcaatgtt caatgccatt ataatttcct ccgtttaatt gttctttttt atttaatgct
    11221 ttatgcggca attaagctgc atctccatct tctttgtctg ccactgcttt gacagcgtaa
    11281 gccacgttgc ggactggcgc ttgtagtaca gatagcaaca tagatagaag tccttcgcga
    11341 tttggcaatt ttgccaatgc tgtgatttct tccaatgaag agactttacc ttcgatgata
    11401 ccgcctttga tttctaatgc ttttgcttct ttagcaaatt cgtccatgat tttcgctggt
    11461 gcgactacat cttcgttact gaatgcaaca gctgtaggtc cagtgaatac ttcgtccata
    11521 ccttcaaggc cagctttttt agctgcacgt gaaagaatag aatttttgat aactttcatt
    11581 tctacgttag catcacgaag ttgtttacgt aaacgtgtta cttcatcaac tgttaatccg
    11641 cggtagtcaa cgactactac tgatgcagct gctgtaaatt tttcgcttac ttcatcaacg
    11701 attgctgctt tttttgcgat aattgcttca ctcatttttg agtttcacct cctgaatttt
    11761 gatggaagag ttttttgcac tgaaaaggca agtcactttt atagttactt tgctcaacca
    11821 gtattattca tcacctattc acttcgttca tagtgattct actcgctttg ctcgaaagga
    11881 aactcctttc gctttgctca accagtattg ttcatcatct cttcacttcg ttcatagtga
    11941 ttcacaataa aaaactctat gccaccgcag acatagaggg actattgaat atattcaata
    12001 aacgtcctcg gtaggaaatt aaggcttgat gccacctact gtcttcggta caagttaatt
    12061 attaacctca actagcttag catgctaaac tagttgagtc aagaattatt tttcagttgg
    12121 atcaatcaac attctaatat ttaattagaa agaagcttgg tcaacatgga taccaggtcc
    12181 aaatgttgtt gtgatcgtta catttttcat gtattgacct ttagctgctg atggtttagc
    12241 ttttagcaat acatcatgga ttgctgcaaa gttttcaact aatttttcat cgtcaaatga
    12301 aacttttccg attggtacat ggatatttcc agctttgtcc acacggtaag ttactttacc
    12361 agctttcact tcgtttactg cttttgttac gtccattgta actgtaccag ttttagggtt
    12421 tggcattaag ccttttggtc ctaatactcg tcctagacga cctacagtag ccatcatatc
    12481 aggtgttgca acaactacat caaagtcaaa ccatccgttt tggatttttt gaaccatgtc
    12541 gtcgtcacct acgaaatctg caccagctgc ttcagcttct tttgctttgt ctcctttagc
    12601 aaaaactaaa acagtttgtg ttttaccagt tccgtttggt aatacaacag caccacggat
    12661 ttgttggtct gcttttttag ggtctacgtt caaacggtat gcaacttcaa ctgttgcatc
    12721 aaattttgca atgtttgttt cttttgctaa agctactgct tcggcaacag gataaacttt
    12781 acttgaatca acttttttta atgcatcttg catttttttg ctctttttag ccattttggt
    12841 tcctccttga ttgtggttat aacggttgta cctcccacgt gttctatatc tttcaatata
    12901 gagccaactg agaagctgct gtcttagggt gtatagatta ttctacagtg atccccatgc
    12961 ttcgtgcagt tccttcaacc atgcgcattg ctgcttcaac gttagctgcg tttaggtctt
    13021 ccatttttaa ttcagcgatt tctcttactt gatcgctaga aactttcgca actttatttt
    13081 tgtttggttc gcctgaacct ttttcgattt tagctgcttt tttcaataga acagcagctg
    13141 gcggtgtttt tgtaacgaat gtgaatgaac ggtcttcgta tacagaaata acaaccggaa
    13201 tgatcaaacc tgcttgatca gctgtacgag cgttgaattc ttttgtgaat cccatgatat
    13261 tgatacccgc ttgacctagt gcaggaccta ctggtggagc tggagttgct ttacctgcag
    13321 gaatttgcaa tttaacgatt ttttctactt tctttgccac gagacatacc tccttaagtc
    13381 cgtgatgtgg ttaattggag atgcatattt ctcctcccac gttttatctg atctgaaaaa
    13441 atccagacaa ataatgtgca aaacaatgca cactggtaca gtatagcaaa tttatttgtt
    13501 ttttcaagat ttttttctgc tatttttaca ttttgcttga ataaacgcta aatttattct
    13561 aaaaaaacaa caatttttcg ttctcatttc ttcttttatg ttacacttgc gctagcatgg
    13621 ttcaacagat gaaagcaaac aataacacgg ttttttagac ccttgtacaa agattcctac
    13681 ataacaaaat gaggtggaga aaatgaaaga tttaaggtat agaagtgtct ttgatattat
    13741 tggacctgtg atgattggtc caagtagctc tcacactgcc ggagctgcta gaatcgggaa
    13801 aatcatacgt agtatatttg gcgaacagcc tgattctgta gatatttatc tatatgaatc
    13861 atttgcaaaa acctatcggg ggcacggaac agatattgca ttagttggcg gcctgttagg
    13921 gatggaaccc gatgatgaac gtttagctga ttctttgaaa ttggcatatg aagccggtat
    13981 ggaagtcttg tttgttccta agagtgaaaa agccgaacat ccaaactctg taaagatgct
    14041 tctaaaaaaa ggaacacgaa agctttcagt cactggcatt tcgatcggcg gtggcaatat
    14101 ccagatttct gaattgaatg gattcaaaat ttctttaagc atgggtactc ctaccttgat
    14161 cgttgtccac caagatgttc caggtatgat tgctaaagtg actagtatac tttctgagac
    14221 caatacgaat atcggcacta tgacagtgac acgggaatca aaaggtgaaa atgccattat
    14281 gataattgaa gtagatgatc ctcaagtaga agaaacggta aaaagactca aacagctgcc
    14341 aaatatcgac agcgtaaatt atttcgatta aggagctaaa aaaatgtttt tatcgattga
    14401 agaattagta gatcaagcaa aagcttatcc aagtgtagca gaattgatga ttgcagttga
    14461 aatggaaatg agtggccgtt cccgtagtca catcatcgag atcatggaaa aaaacctctc
    14521 agtaatggaa caatcaatca acgagggaaa agaaggtgtt acctccgtca caggaattac
    14581 cggtggtgat gcaaaaaaat tagatgatta tttaaatgag gcagactttt taagtggaga
    14641 aacaatctta aaagctgtac gcaatgctgt agctgttaat gaagtcaatg caaaaatggg
    14701 gttgatttgt gctacaccga ctgccggaag cgcgggtgtt gttcctggtg tgctgatggc
    14761 tgtccgagat cggcttgatt tgacacatga acagcaattg gaattcttat ttaccgctgg
    14821 cgctttcggg ttagtcatcg caaacaacgc ttctatcagt ggtgcagaag ggggatgtca
    14881 agcagaagtt ggttctgcaa gtgcaatggc agcagcagct ttggtttgtg caaaaaatgg
    14941 gactcctcat caagcagcac aagcagtagc cattacattg aaaaatatga tgggactgat
    15001 ttgcgatccc gttgcaggct tagttgaagt cccgtgtgtc aaacgtaatg ctttaggctc
    15061 ttctcaagct tttatctctg ctgatatggc attagcgggt atcgaaagtg tcattccgcc
    15121 ggatgaagtc gttgctgcaa tgtaccaagt cggacgtcaa atgccgtcga ttttcaaaga
    15181 aacagcagaa ggtggcttag ctgttacgcc aactgctaaa cgacttgcgg aagagatttt
    15241 aaataagagg gcgtgataaa gctccttaat ttcgagtatt aagagattag ttatcttcat
    15301 aacaaaaaac gaagagagga ctaattgccc ttctcttcgt tttttattaa tccatcaatt
    15361 tcgtattttt tacttcatcg atcgtttttg ttcctgcaag ttgcatcgtg atcgacaatt
    15421 ctttgttcag gtgttcaaaa acagatgtta ctccttctgc cccacctaga ttcaacccat
    15481 aaatcactgg tcggccaatc gcaactaagt cagcaccact tgccagagct ttgaagacat
    15541 gctctcctcg acggacacca gagtcgaaaa tcactggtac acgtttgttg actacttcgg
    15601 caatttttgg caatacttca aaggacgcag gacctccatc tagttgacgt ccaccatgat
    15661 tggataccca gataccatct gcccctgcag agatagcgat tactgcatct tctggcgatt
    15721 gtattccttt tacgataact ggaagatggg tgatttcttt gattgttttg atgtcatctg
    15781 gtgtcaagcc ttgttttgca gctgcataaa tttctgcgat tccttttcct tcgccattac
    15841 cacttgcact ttgttcgcta tacgcagcta agttaggcat tggtaatgga aattggaatt
    15901 gattgatgac gtcttcttca cggtaaccac cgagcgttga atctgccgtt aaaatgatag
    15961 ctttagcccc tgcttctact gctttgttca aaatgaactc attgaatctg tcgtctttac
    16021 tcatatataa ttggaaaaac tgcggtgcgc ctggcgctgc atcggctgcg tctttgattg
    16081 tcgtatttgc ataagtgctg atggaaaaga ttgatccagc agcagctact cctttagctg
    16141 tatcagcttc ccctttttca tgtgccaatc cttgggccgc tgatggcgct tggataatcg
    16201 gtgtatctaa ttcaattccg aaaacagatg tatgcaaatc ggctgagtcg attccgcgta
    16261 atatccgtgg catgattttc tttgtattaa aagaagtcgt attttctttc atcgtccact
    16321 catcttcaga accaccgcga atatagccaa aagctcctgc ttccatgcgc tcttttacac
    16381 gaccttctag ttaggcaatg tttacaatat cgattggatg ttcttcatta cttgtatgat
    16441 aagtcatagt agatccttct ttcatcttct tgattacata taaatcatac taaacttaca
    16501 aaaagtaagc aaacatttac tctcagatac ttttatcgct attctttcca aataaatcga
    16561 cttacaagct gatctacttc ttggttttca tgaagcatac tatgagaggc attttcacct
    16621 gtcactactt tttcctctat ttcatttccg cgttgcttca acaacgcata gaccgctaat
    16681 gcactgttta acggtactgt tccatcactc aagtctgttt catctagttg accagcaatc
    16741 aagaaaaatc gagtggttat aggaatatta ccgatcattt gctggtaatc ttgatagcgg
    16801 ttagaaacga cagcaggccc ctttgctaac tcatctgtca gagactgcgc actatcatcg
    16861 acaaaatcat taaaaggcgc gccaattgct gttaatgtct gaacttgtgg caatgacgta
    16921 tcctgtccat acgttcctaa atagcgtagt gcactaactc ctcccatgga atgccccact
    16981 atgttgactt tatctatgcc gtagttttct ttcagataca aaagacaagt tttgatccat
    17041 tctgcttgat tccactcgtt atttttatta tcttcaaaca atacttggac catcggattg
    17101 tctgcttttc cagacaaatc tcctgttgct tgtatttgtc catctgttcc tactgttaaa
    17161 accaattctt ttactgcttg ctgatttttt tccaaacgcc gcaacatccc tttaaaggaa
    17221 ttttctgtcc cgctgtagcc gtggaaaaaa agtgttggaa tagtggtttt ttctatcact
    17281 ttactggatt ctgccgtttt ttctttatca gaaacttcag tagtatttat cttcgtcgtc
    17341 tggcaaccac ttaacgcaaa caaactgata aggactaaag ctgctgcagt tgcacgtttg
    17401 cataaatttt tgattggttc tgtcatacac ctcttccttt ccatatagaa aaaagaaaga
    17461 cttgaaaggc caatcatatg atcgacattt caagtctctt tattaatcga ttttatctac
    17521 ttgatcgaaa tcaagttctg tactggtttc acgtccaaac atatcgatgt tgactttcaa
    17581 tttttctttt tctccgtcaa cttctgttac ttgtccttct aatccagcaa atgcgccttc
    17641 gatgattttc acgacttcgc caacttctac ttctagatca ttttgtcgca ggctcatacc
    17701 gatagaacgc aagatatgat tgacttcttc tggaagtaat ggtgctggtt tgcttccggc
    17761 tccgtgtgaa ccaacgaatc ctgtcacacc aggtgtgttg cggacaacat accatgaagc
    17821 gtctgtcatg atcatttcaa caagtacgta tcctgggaat gttttatgaa cgatttcttt
    17881 ttctttccca tttttgactt cagtttcttt ttcttcagga acaacgacac ggaaaataaa
    17941 gtcggccatt ttcatacttt gcgcacgaga ttcgatattt gcttttactt tgttttcata
    18001 tcctgaatat gtatgcaaga cataccagtt tttttcaaaa gattccattg aaggctcctc
    18061 atttcattgt aaaataaaaa aacctcagta cacccgaagt ttttctttcg cttcattata
    18121 gcactaaaag tacgttgtgg ctagcgagtt attttaaaat ccagccaaat aagctttgga
    18181 tggctgtatc cattacgtaa aacatcaatc cgaaaataat agaagtttcg atgactacta
    18241 acgtatcttt acggagttgt tttctgcttg gccaagttac ttgtttgatt tcatctttta
    18301 cgctttttag aaatttcatg agacaacctc ccttgctact gtttatttcg tttctttatg
    18361 taatgtatat tgattacaat atttacaaaa cttattgatt tccaaacgtt cgccgctttt
    18421 tccttcactg actgattttg tgtagttgcg agacccacaa acggaacaag cgagtgctgc
    18481 ttttttcttt gccatttact tctgccccct tgctgagaat cagacttata cttcataaca
    18541 gtatcacgta actttttact tgtcaatttg tgaaataaac tatctattgt tttggataaa
    18601 attatgatat gattaatttg aaattaagct aaaatgagac aattggaggt atagcgatgc
    18661 tattaaaaac acttgtatat aagaaaaaag atctgacaac cgttacagag actagtacat
    18721 tagaagaagc attaaaaatt ttagaaactt ccggataccg ttgtgtgccg atcttagatt
    18781 cttcaggtaa tattttccgt ggaaacattt acaaaatgca tatctatcgt cataaagcaa
    18841 atggcggtga tatgacgctt cctgtcactc atcttttgaa aaacgctaca aagttcattt
    18901 acctggatac ttctttcttc aaagtattct ttacgatcaa agagcttcct tatattgctg
    18961 tccttgacag tgaaaatcat ttttatggta ttttgacaca tagtacgttg cttaatatgc
    19021 tctctcaatc ttggagcgta gaacaaggaa gctatgtact gacaattgct tccacaggaa
    19081 agcaaggaga tttggctgct attgcaaaaa tcatttcaaa acacagcagc attgccagct
    19141 gtattactct tgatatcgaa aaagacgaat acattcgacg tacattgatc acgctaccgg
    19201 ctaacactga agcgaaagta tgtgaaacga tcgtcaacca cttagagaaa aagaattata
    19261 aagtggtaga aattgaaaat ttgcaaaacc aagaagcata atcaaaaagc gaaaaaagaa
    19321 aacagctaat ctgctttctt ttttcgcttt ttataattag acacgctttt caaaatcaag
    19381 cgcgcgatct aaataataca tcaatggtgc tgaaacagcc atgacgataa actcacttaa
    19441 tgcaagcgtg ccataggttg tccaaaatgc ttggccacct gttggaacta acatataagc
    19501 aatcagtccc atgctgatgg taaagaagat cgtgttcaat actaaacgca tctttacatt
    19561 tttcactttg ttttgcaata aagctgtcag gcctaaagaa agcaatgttt ggccgccacc
    19621 aaaaaccaca tccatgatac caaaaccaaa gaaggcatta tagacgatga ccccgcctaa
    19681 aactccccaa agcaatttac ggttaaagac aactaaatga ttcaaacttt ctgaaatacg
    19741 aaactggatc ggccctgagg atacaggcgc aactaaaata gttaacgcta gataaagagc
    19801 catgatgatg ccatttacaa cgattacgga taaccgttgt ctgcttttcg ttttttgcat
    19861 tttctctcct cttttctcct agttttttta acgcgggatg gttgatgaac cgcggttatg
    19921 tgaaacaaac aataattata acagactttt catttatttc aatccgtctt atctgctttt
    19981 aaaaaagcaa aagcttaaac aatgtaaacg tttgctaaaa atcgtgggat atggtataaa
    20041 gtaagtgtaa ggggtcataa gaattcccag taaagcagac gaagtaggat ctgcaagaac
    20101 agaattgttg tgaaacttaa ataaataaaa aaggatgtgg gaatttgagc aacctcttac
    20161 tagagaacgg tctagcaagc agctgaagat ctaagtggac atgccgaacc ttggaacttc
    20221 tccttacgta agtaatggat catgcgcttg ctaggctttt ttgtgttttt cggtcttcgg
    20281 acctttttca ttttcctaga cttctgctat aataaccaag aattaatttt gtaacagatt
    20341 tttttgaaaa aacaagtttc tttcattcaa aaaatagaaa aagcttgcta attattggtt
    20401 aaccagcaaa caggagtatt aaaatatctt gttttattga atagcaggag gaaatcatga
    20461 aattccatta tcatatattg atcaatccat cggcaggcag cggaaatggc tataaagtag
    20521 cagaaagaat tttaccggta ttaaaaaata agcatattga ctatacactt cattatagtg
    20581 aatacaaagg acacgatgct gagattgctg aaactttggc aaaagaaaca ttgattcctt
    20641 ggatagaaga agaacagaaa acaatcgata ctttccccct tttgatcatc gtaggaggtg
    20701 acggcacctt acatcaggta cttgatactt tctatcagat ggaagtagaa ttcccagttg
    20761 cttatattcc agcaggttct ggagatgatt ttgctcgagg ggcaggcctt ccaaaaaatc
    20821 ccaaaaaagg acttcagctg attttagcag ctcaatcacc agaaaaagtc cacataatgg
    20881 cttatgaaga aaaaataagt gaaaaaaaag gaatagctgt taataatttc ggtataggat
    20941 tagatgcagc tatcgtccat gcgacaaatc attcttccac aaaaaagcga ttgaacaaat
    21001 acaacttagg atcattttcg tatttattct ctattctccg tgctttattc actcaaaaag
    21061 gatttccaat tctggtagat gcaggaggaa aacaattaag tttttcaaac gcatttttat
    21121 gtacagcaac gaagcatcct ttttttggcg gaggaatcgc gattgttcca actgctgatg
    21181 cgtctaaacc agtcattgat tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt
    21241 ggctcattct tttacttata cagaaaaaac aattgaaatc gaaatatttc catcactaca
    21301 caacaaacag attacgtatc gtttcaacaa caccgcaaca tggtcaagaa gatggagaag
    21361 aaatggaaaa acgacctttt gatatcacaa taaccacaac taaacagttg ttttggtgtt
    21421 aataaaagag gttttgtcag aagctacatc tggaacaccg tttattcgga aaaaaggact
    21481 gtgacacgac ttttgtcaca gtcctttttt tactatgcag ttaattcttt gcccaattgg
    21541 gttgccatcg atagtttctt tctttaccca aaataaattg gatcattgaa aacaccgcag
    21601 caaaagcaac taagacacaa ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac
    21661 tgagtgtttc actgatcaag ttattatttt gaacaaacca atatgttggt gtgaatgccg
    21721 ctattttgct aaccatttct ggcaagtatt ctgctggtac aaacacccca ccgataaagc
    21781 aactgcctgt gataaaaata tctttgatcc caccgctggt ttctgagttt tttaccaatg
    21841 aagtgataca agccgaaaag ctaatgatcg gcaataaaaa caagaaagta tttaaaatat
    21901 gataccatac cgttatatcc cacgaggtgt ggatcactaa cagaaccatt agaagtaaga
    21961 agccactgaa caacagcagc gagtaactaa tcaaactaaa ggttactttg cgattcaagt
    22021 ttcgtttggt aatgggcgca ctctggtttc gtttactgat ctctttgcga ttaaatgcta
    22081 gattgatgac gctgaaaccg ctaaaaatgg atagaaacaa cccataagac aagaaattaa
    22141 aggttgtgcc cgtcaacagc tttttcatcc gctgactata tcccgcactt agctcaactt
    22201 ctccagtaac cgagagtgcc atttgtgtct gttccatagc tgcatcgatt gttttatctt
    22261 cttcttgaaa cagaatcagt gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt
    22321 tagaaaaggc atctgggcga ctctgtatct tgatcgtcgg catctttcct acttcgactt
    22381 gttcggtaaa gtctgcaggc agatgtaaga taatgtttac acgattaaaa taaagagcat
    22441 catcgatttc tttttgtgag gttccatcca atgtaacgac tgtttccttg tcattcaaat
    22501 atttacgaag gccatcgact acaggcgact cttcttcact aatcaaggcg attttcgcat
    22561 cttgcagaga ggtattttca acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag
    22621 tgataattac acctatcagt aaattcgatt tatttgcttt aagcaatgtc caaatcgttt
    22681 tataaagcgt cataacgcac ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa
    22741 ataaatccga tcaaccatag aagattggta taaaaactag ccaaatcagt gtagaaataa
    22801 agttt
//
LOCUS       DARBFD010000042        22295 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000042 DARBFD010000000
VERSION     DARBFD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.042"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..352)
                     /locus_tag="SCK08_001495"
     CDS             complement(<1..352)
                     /locus_tag="SCK08_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG1201379.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQ"
     gene            1046..1432
                     /locus_tag="SCK08_001496"
     CDS             1046..1432
                     /locus_tag="SCK08_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1201380.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1526..1717
                     /locus_tag="SCK08_001497"
     CDS             1526..1717
                     /locus_tag="SCK08_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201381.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2231..2563
                     /locus_tag="SCK08_001498"
     CDS             2231..2563
                     /locus_tag="SCK08_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1201382.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2859..3014
                     /locus_tag="SCK08_001499"
     CDS             2859..3014
                     /locus_tag="SCK08_001499"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG1201383.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3290..4630
                     /locus_tag="SCK08_001500"
                     /pseudo
     CDS             3290..4630
                     /locus_tag="SCK08_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4871..6487)
                     /locus_tag="SCK08_001501"
     CDS             complement(4871..6487)
                     /locus_tag="SCK08_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1201384.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6605..6823
                     /locus_tag="SCK08_001502"
     CDS             6605..6823
                     /locus_tag="SCK08_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201385.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            7018..7479
                     /locus_tag="SCK08_001503"
     CDS             7018..7479
                     /locus_tag="SCK08_001503"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1201386.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7672..7911)
                     /locus_tag="SCK08_001504"
     CDS             complement(7672..7911)
                     /locus_tag="SCK08_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201387.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7935..9458)
                     /locus_tag="SCK08_001505"
     CDS             complement(7935..9458)
                     /locus_tag="SCK08_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1201388.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9476..9598)
                     /locus_tag="SCK08_001506"
     CDS             complement(9476..9598)
                     /locus_tag="SCK08_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1201389.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9628..10611)
                     /locus_tag="SCK08_001507"
     CDS             complement(9628..10611)
                     /locus_tag="SCK08_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1201390.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10635..10784)
                     /gene="rpmG"
                     /locus_tag="SCK08_001508"
     CDS             complement(10635..10784)
                     /gene="rpmG"
                     /locus_tag="SCK08_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1201391.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10805..11182)
                     /locus_tag="SCK08_001509"
     CDS             complement(10805..11182)
                     /locus_tag="SCK08_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201392.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(11214..11393)
                     /gene="rpmF"
                     /locus_tag="SCK08_001510"
     CDS             complement(11214..11393)
                     /gene="rpmF"
                     /locus_tag="SCK08_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1201393.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11408..11677)
                     /gene="rpsN"
                     /locus_tag="SCK08_001511"
     CDS             complement(11408..11677)
                     /gene="rpsN"
                     /locus_tag="SCK08_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1201394.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(12200..13966)
                     /locus_tag="SCK08_001512"
     CDS             complement(12200..13966)
                     /locus_tag="SCK08_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201395.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13926..15679)
                     /locus_tag="SCK08_001513"
                     /pseudo
     CDS             complement(13926..15679)
                     /locus_tag="SCK08_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(15789..16358)
                     /locus_tag="SCK08_001514"
     CDS             complement(15789..16358)
                     /locus_tag="SCK08_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEG1201396.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(16376..17791)
                     /locus_tag="SCK08_001515"
     CDS             complement(16376..17791)
                     /locus_tag="SCK08_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1201397.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(17802..18470)
                     /locus_tag="SCK08_001516"
     CDS             complement(17802..18470)
                     /locus_tag="SCK08_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1201398.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(18600..19184)
                     /locus_tag="SCK08_001517"
     CDS             complement(18600..19184)
                     /locus_tag="SCK08_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1201399.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(19515..19748)
                     /locus_tag="SCK08_001518"
     CDS             complement(19515..19748)
                     /locus_tag="SCK08_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEG1201400.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(19763..20713)
                     /locus_tag="SCK08_001519"
     CDS             complement(19763..20713)
                     /locus_tag="SCK08_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1201401.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(20710..21552)
                     /locus_tag="SCK08_001520"
     CDS             complement(20710..21552)
                     /locus_tag="SCK08_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1201402.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(21554..22273)
                     /locus_tag="SCK08_001521"
     CDS             complement(21554..22273)
                     /locus_tag="SCK08_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1201403.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         7318 a         4173 c         3184 g         7620 t
ORIGIN      
        1 tttgcaatga attatagagt cgttctctat cagccattgc aacattcaaa ttgttcattt
       61 ttagtttaaa aaaaacttta tcaatattat ttgctctaaa tggaccaaat aagtaatatc
      121 tggttttaaa catgtataga agaaacatct cattaaaatc accagtaaag aaatggaaat
      181 ctttagtttt tttaatatta tcagttagta actttttata atcataaaaa aaataagtag
      241 ttctatctga gcgatactct tttataatac tgaattcgga attaaaaacc acgataggta
      301 actttaaaca aacatgaaga gactttaata agacttcttt atctgaaaac acttttgttt
      361 ctctctttcc tttctacatc attaaatagt attttatacg ttatctaaaa tactattcct
      421 ctgtcaaaat aagctaactt acttcattat aaaatatcca cttatcgctt tcatgtatat
      481 tccaccgtaa aaagtacatt ttatcaccga gaactcatca tttttcatca tgatgaatac
      541 tatttacata tatctagttc aaagacaata attattatga tccgttatta tctaagcact
      601 atacagataa taggaatttt tcgtttctaa atattccaat ttgggaatcc aataaaaatt
      661 ttcgttcaaa tacgtgtcta ttcgaactct tttctagttg ttaattatga agggggaaga
      721 aataaaaaat acaatctata cacgaataag aatattatcc aaaaggaggt tttagaaaag
      781 atttattata cgtaaaaagc tcaagacatt ttcttagcca taaggacatg tttgatattt
      841 aagaagatat atgaattttc ttttctaagt ataaagataa ctatccatta tcaactaaaa
      901 aaacattttt gaaaataaac atcagcagtc ctatcaatgc atgtgagctt atagaagtta
      961 ttcgcagcgc agcataattg cattagcttc ttttcgtgtt ttttgcctct tttcccttta
     1021 aatcttaatg gaggaggtgt gcaatatgtc agataacgac gaacgcgtaa atgcccagtt
     1081 gattcgattc tttgaaaagg tcattttgaa caccgctaat acttattata aaaaacaaga
     1141 agaaattcaa aaacatgaac aacttgatca actcccacca tatttgttta ctaaagatac
     1201 tataaaattt aaacaaccga ttaatgtttt aaatgtgact ctccttgttg aagatataaa
     1261 attagcaaaa atccttcctt tgttgaaaga acctgaacaa atttttctta tcgaaaaatt
     1321 tatctttgat aaaactgata aagaaattgg aaaggttctg gggattacaa gacagggtgc
     1381 gacaaattta aaacatcgac tttacaaaaa aattcaaaaa atgctggttt aatatctgca
     1441 taataactaa ttttgtatgc ttctttgctc gttctcctcc ttcccaaaga aatagattat
     1501 aagtccatgg gaagaaggta gatttatgaa ttctttaaaa gaagatttca ttttagcaaa
     1561 ggctggaaat gaagaagctg ttgaggcaat tctcaaaaga tttagctctt tgattcataa
     1621 gcaatcctgg agaactggaa aatatgatca agattgttat caagaatgta tgcttgcaat
     1681 atatctagct atctcgaaat tcgaaattaa agaataactc aactaaggga gtctgtgaca
     1741 taagtaaaaa gatagtttca aatttcgaac aataaatttg aaactatctt ctttataatt
     1801 ataaagtatc gattttacag cgttctataa tgattcatag aatctaaaac gaatgatttt
     1861 aattatcaac gttttttaat tcgtttttag cttaaaaata acttttgtca cagtctcact
     1921 cctaaaatta ctttatttac gtatgaagtg ggcaggaggt cgaatccaga actgggctat
     1981 ggtacttcaa cgaggacttc tcctctagaa ttgaaaagca tgcgatttac cttccgtaac
     2041 gacggtagat ttattcaact atgtgtccaa tcaaaatttg tctgatttca aaaatagtca
     2101 agagaatttt ggttgttaaa aactcttatc tatcaacttc cacagtttag ttgacaaacc
     2161 ctgatactaa gttgggctat gatacacttt tcttgactat cagttacgtt accggaagga
     2221 atgaactcta ttgagaaaaa catatgatcg taaatttaaa ctaaagattt cacaggatat
     2281 ccttgagaaa aaagtaacta ccaagaaaat tgctgaagaa tacaatatct ctcgtccaac
     2341 aatttcccga tgggtttcag aatatcgtcg atacgggaaa aatgcttttg ctggacaagg
     2401 aaaaagatta cctgataaag cagatttcta cattcttgaa caggaaaata aacgactaaa
     2461 agaggaagac gagtgttcta cgaacacaaa gatcgatacg gatctccagg gatcactcaa
     2521 aaactttacg atgaaggaat cgaaatcaat aaacgtgttg tagctgttct tatgcgtgaa
     2581 atgaatttat gcacaagagg atttcatcga cgcaaatctt ctcatggtag agaaaaaaca
     2641 atcaaagaaa tggtaaaaaa atgttgaatc gataatttga acagaaccaa attgatgctg
     2701 tttgggtgat ggatattact tacattccat gtaatgacgg tcgcctctat ttgtccacct
     2761 atattgactt agcaactcgt attctacgtt actatatggt agattcacac atgaaaaaag
     2821 agattgtcgt ccgtccatta caaatttatt aaggagaatt gcctacggta attcattccg
     2881 atagaggtag ccagcatcgg tcttttattt atcaagagct gttgaacgac caccacatca
     2941 cacattcagt gagccaaact gggactcctg ttgacaatgc agtaattgaa gcgtttcatc
     3001 gatcaatcaa atgagaatta atcgactcca acaaatataa aagtaaaata gagatgaagg
     3061 tgttaatcca agattattta gaagactact acccaaacaa aagaatccat acaaaattta
     3121 tgatgactcc ttgacaattt gaagcgaaga aaataaggga tctataatat ctagtgttgg
     3181 tgacgccttc tgttgccaat actttttctt ttttctaata aacgcaaaca gacaccttaa
     3241 aatcctttag aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga tgtcctattc
     3301 agagtttacc aaagagttac tagatattct agacctaaat cttatctttc atgaagatgc
     3361 ctttcgcaaa gagcgaatca atgacgaaac atgttttgtt tttgatggca cattgaccta
     3421 tcaaccggag gaatgctttc atcgccacta tcaaaatgaa caaacgatca ttaaataggg
     3481 ctggaaaaaa gtatccatct tattgaatga tgtcagtaac tacaagacca ttctgcggat
     3541 taacaaacag cgcttcaaat gtaaacactg tggaaagact tttttagcag aagattcagt
     3601 tagtgatcgt cgctgctcca ttgctcgcag agtcaaacaa gccattcttg agctattaag
     3661 cgaacctctt tctatgtctt tgatcgcacg tatgaagcac atttctccga ccactgtcat
     3721 tcgaatactt cgcagccttc ggcctaaaac catttctttg agccagctgt taccagaagt
     3781 ggtgtgtttc gatgaattca aatcagtgaa aaatgtctcg ggagccatga gctttgtcat
     3841 gatggacggc aagacccatc aactgattga tattgtggaa aatcggcaac tcaatccttt
     3901 acgcgactat ttcttgcgct accctataaa agtccgtgaa caagtgcgtc tagtggtttc
     3961 agatttctat acaccctatc gcacattggt gaaagagtgt tttccgaatg cccgaatcgt
     4021 cgccgatcgc tttcatatta gccaacacat tggtcgggct tttaccaacc accgaattca
     4081 agtaatgaag acctttaaaa aaggggatcg tcggtcgaag cacttaaaga aatactggaa
     4141 actacttcaa aagaatgcgt gggaattgaa aggtcagcat cgctactggc gtccgtcatt
     4201 tcgagatcat ctgacagaag cagagatagt cgatcggcta ttatcttatg atgattcctt
     4261 aaagcgaggc tacgaagtct accaggattt tctttccgtt atcagaagac aagatgttcc
     4321 tgaattcgtc gcgctcctaa aagaagacta caaggagttg ccagagcact accaaccagt
     4381 gttcacgacc ttcaagaaat accaaacaga aatcaaacga gcgttgcgcg ttccctattc
     4441 caacggccct atagaatgtt tgaataacca tatcaaagta ctaaaacgaa ttgcctatgg
     4501 ctatagaaac tttcaaaact accgagaacg aatcttttta tatcgaggaa aatattttaa
     4561 gaaaactaag aatactaccc aactgactaa agccagaaca acaacacggc tagacaagat
     4621 agctgtctag ccgtgtgagt atctcgatta ttctattgaa tttcttcacc aacactcatt
     4681 gacgaagagc caaaataagt aatctaaccg actgttaact aaaccattgc aggtcccgtt
     4741 aaagatactt gtgcctgttg tcaccgcttc tttttcttgt ccacaagcgc ttaacacact
     4801 taataaagcc cctgctatta ttaataataa tatctttttc ttattaaaaa cctcctcatc
     4861 tatttccaga ttagtaaatg tagtaatctg tgctttcttt tttctcaata ctaataacta
     4921 acttatgggg taaacaaata ctcgtttgac cgggttttga gatccatcca gttctaaccg
     4981 cgatttgatc aggactatta tcttctttaa ctcgaattct tccatcttta atctcaacaa
     5041 tattatattg tcccttcgca ggatgataat tcacgagttt atgattgatc ttatccaaat
     5101 cgaagcgatc catttccttg ccatcaattc gaacgatggc atatttgata gcatcttcag
     5161 ttgtttcatt ctccttttct atagagaata caaataaggg aataaaagag gtaataatca
     5221 aaatgccaat aattatgaca tcgcctatct tgacaccagc ttttcttaaa aatttcatta
     5281 acgatatctg gctaaacata ttagactttt tccatggaaa tggtgaaatt tttcggaaat
     5341 tatctgcatt aagagaatca gttaaacaac cctcaagttg ttccttaata cattgttgat
     5401 ccatcttcat accaataaaa actagtttat taatgcgttc accaaacaac ggatgccagg
     5461 cttccttcaa ttcgggattt ttttgtataa tcatttgaca ctcaatttta ggtaaactag
     5521 ctacccaata atttaatgat tcaatacttt tatagctacc cgatgtttca aagatcgtcg
     5581 aaaaatcact atgttgggca aaccaaagaa acccttttgc tcttgtaata ttgccaggaa
     5641 aatttttatc cgtaagatgg gcaaattttt gtgcgtcaag gagtaaccga tttcgataga
     5701 taaaacttga gataccatat tcttctgttt caggaatatg attttgatag ccaagctcta
     5761 actctttgaa ccagcccact gaataggaag tctcatctat attaaaaaga tttgtattta
     5821 gaattttcga taagtctact tcacctttaa ttgtcctaat tattttggca ttggactgta
     5881 acttagcaat tagaccgtta attttgtaaa tcgtcttggt tgacactaag tcactattat
     5941 ttaaaagtaa tatgttacaa cactcgaatt gatcaattaa caagccacga atatcctgtt
     6001 cttcttcaac tacattacct tcagcttttc tttcctgaat cttttcaccc gtttcgaaat
     6061 tatcccaaaa acgttttaca tccactacta taataatggc accaatctct aatcgttggg
     6121 tcaaatcaat gtctaaaggg tttttagcta atataattgc ttgaataatt ggaattggtt
     6181 tgctgattcc tgaagcttca ataacaattt gattcaagtc agaaaattgc actaattgat
     6241 ctagttcaac aatcaaatct tcgcgtaatg tacaacaaat gcatccattc ggcatcgaaa
     6301 caagtttttc tccagttctt ttaaaacctt tcttgtcgac taatacccca tcaacattta
     6361 cttcccctat gtcatttaca atgacagcaa tttcctttcc gtgatcaccg tacagaatat
     6421 gatttagaag tatcgttttt cctgaaccaa gatagccagt taagattgta ataggtattt
     6481 ttttcatagg gaacctcttt ctataccttg aatttacact ttaagtgcaa cacataaaaa
     6541 tgagtcataa tcgatatcca actagtataa gtttgtcgag aacactccta gaaggaaaat
     6601 aattatgagc cagtatcgtc atcttactat actcgaacga gaaagaatct ttctgcttca
     6661 cgaagaagga ttttctatta gtataattgg caacgacatt cagcatagcc cgtttactat
     6721 tttacatgaa ctgagaagaa acaagaaaaa aactattcac cttccaaagc ggaaaaaaat
     6781 tatgcttatc gaagaaaaaa atgcggacgt tatcacattc taaatgatcc gtgagtttgg
     6841 tcaattgttc gtcatttatt tgtcgatttt cagtggtcgc cagaggaaat ctcgaatcgt
     6901 ttgaagcatg aacatatgga cttttcaatt agttttatca cgatctaacg tgcaatttgc
     6961 aatgatcttt tcgatacaga acccctctca cacggcaata aaggcctgat ccgttcattg
     7021 caccatcgag gaaaaacaag acatacaaaa aatcatgtcg agtctcgtgg aaagatttgc
     7081 cttactcatt cgatccatgg ccgtccaaaa tccgcagatg atagaagtga ttttggtcat
     7141 tggaaagccg atattgtagc tggcaaaact ggtggtactt gtttagtgac tttgacagac
     7201 agaaaatcat gcttcctttt agcaggaaag aaggtcccca gaaagaaagc cgcttatgtg
     7261 agcgagaaaa tgattgaact tttaagcagt attccaaatg aggcggtcaa aacgatcact
     7321 ccagatagag gaaaagaatt ctccaatcat ttcgcagtta cagaagcatt gaataatatg
     7381 cccttttatt ttccagatcc tcatgccctt ggcaacaatg aacaaattaa aatacgaata
     7441 gactcattcg agaatacttg ccaaaatcga aagagatagc tacggtagat aactcaacga
     7501 taaatcagat gattgaaaaa ctgaataaca gacctagaaa gtgtttagat tggagaacac
     7561 cttatgaagt cttctttgat aaagtgttgc gcttgatttg acaattcaag acataataaa
     7621 aagcagatca aatccaaata agggaaaaat gatctactct ggtattatca attaattggc
     7681 caagctatta tgccgttttg ctaaacgtct acttcttcca gtattgacct tgataaaaca
     7741 acaatcagga tcccgtttta atactctatg aactgtgtca atactaacat gtaaagttgc
     7801 agcaatatca gctaatgtac tcccatttaa tctcatttgc tttgcaaatc gataacgccg
     7861 ataagcatca atattcttgg aatccataga caataatact tttcttacca tgtatttcct
     7921 ccaaaaattg aattttaatg agccatcatt tcttgagcaa tttcatgttt agacaatgaa
     7981 gctggataga aagtaggcca attatgcaat tcattataga gtttttcttg actttctcca
     8041 ccaaagaaaa tatggaaatg ctctgcctta cttggtgcaa ttccatgatc actaaattga
     8101 atatatttaa aacgaccagc acctgcatca tcagtttcaa aattaaaacg aacaccacgg
     8161 tttccttttt cataattcaa aattttgtat ccaacatatt tgtatgtgta ttgatgatgt
     8221 tcaccgttga ctacaaaatc aatggtgttg tctttaatgt tgatagtatc aatatctgtc
     8281 ttatagccag tagtataata atctttgtac tcggcagctg tcatgtcttt attgattttt
     8341 gctttgtaat cgaaaacttc atccaaaatt ccatctttta acaacggata aacagattgc
     8401 cattcgcctg tataatcact caattcgcga tccttcacat cggcatcttt aaagtaccca
     8461 ttagcaactg tcttttcatc cttcgcttct ttttctggaa ctacctcttc accagcaacc
     8521 atagttgttt tttctaacgc ttttagattg tcttccatta ctgaaacata atcttcacca
     8581 ttatccattt gttcttttgt aagactttca agaggattca gtacttccaa ttttatccca
     8641 gcttcgttgg ccaaagtttt tgcaatttta tcattagcat tcttttcaaa gtaaatataa
     8701 ttgatcttat tttttttaac atactctttt aattcagcta atcgaccaga agatggctct
     8761 tcttcaggac ttagtccagc aataggaact tgtttcaatc cataatccaa agctaaatat
     8821 ccaaaggctg cgtgttgcgt cacaaaactt ttttgtttcg cttctttaag actagtggta
     8881 tagtcagcat ccaaccgttt cagtttagtc aaatactttt cagcatttgt ctcaaatact
     8941 tttgcttttt tcggatacaa tttaactaac tgatccttga tatttgaaac ttccttaatt
     9001 gccattttgg gtgaaaccca cgtatgagga tctagttcat gatggtgatc ttcttctccg
     9061 tgatcatgat cttcttcttc actacctggc aataaaatca tgtcttttgt tccttctact
     9121 acattaggcc cagctttctt ccaactttct ttagcttttg gtacccatga ttccatgttt
     9181 tcgttatgat atacaaaaac atcagcatca tgagcttttg ccatatcctt agctgagggc
     9241 tcatagtcat gaggttctga tcccgctgga accattaaat ccacgttcgc ttcatcgccg
     9301 acgatgtttt tggtgaaatc atacattgga tagaatgttg ttacaatttc taattttcca
     9361 tctttatcat tcgatacttt tgcatcatta gtgctggagc aagctcccaa taatcctaga
     9421 gctgctatcc caataagtcc tacactgatt ttcttcattc tttttccttc cttaattatg
     9481 ctaatttctt cattttattt cgtttatgtt ctttgataat ttttaatgcc ctttttctag
     9541 ttttaatatt cgggctttta agtcgtcgat atgcactaga tatatccgtt ttctccatta
     9601 gaaattaccc ccaatcacta cttatcatta tttgctttcc gccagtaatt tatcaaaact
     9661 ctgtttgatc ctttcttcat ccaacatttt tccgattaga ataatagtgg tttggtcttt
     9721 ttgtcgagtg gtgtattgaa aattaacctg ttcgtttacc tcttgcatgg caactataaa
     9781 atcctgctct tgtaaagcaa tcaatccttt aaaacgatac aggctccctt ggttggttaa
     9841 aatcagccag tcaatccatt ggagtaaaaa atcttttttt atgtcttgat ctgaagttaa
     9901 gagaattgat ttaaactcat gtacttgatg gtgttgatga tagtatttgc cattatagtg
     9961 attttctgat gaatcacttt caaaaactaa gggatgatta aattttttca aattataaaa
    10021 atcctttttt tgaatcgttt cactatgtgt gaacattcga atatcggata aagggttaat
    10081 acttttgatt tctctctgta atttttttag tgcagataaa ttcaagtttt gttttctaga
    10141 cacaaaaatg cgatctgcca tgacaagttg cttaagggct tgtggttctc tttcaataag
    10201 ttcaaaactg tcactatcaa taacagtcaa taaactatcg atatatactc ttcctcttag
    10261 ttgaggcgct gttaaaagtg tttgaatgat tggttgggga tctgctatac ctgtagtttc
    10321 aatgatgatt tgtgaaattg gttgttgatg ttcttctatt gtttccagta aagaaatcaa
    10381 tattgctaat aaatcactgc gaagactgca acacatacat ccattattta gttgtaacac
    10441 ccgttcctcg ctatgaacta ataatttgtg atcaatccca acttcaccaa actcattttc
    10501 aattataata atttcttccc tggggtaggg tgagacttgc aaggcttgat tgattagcgt
    10561 tgtttttcca gcccctaaaa acccagaaac gattgtaact ggtatgccca ttttgtcacc
    10621 tttttaccac ttagctattt cacttcttta aaaattgccc gtctgcgcaa ttttggcgaa
    10681 tactttttta attctaaccg ttcagggtta ttccgtttat ttttgctggt gagatacaga
    10741 cgttctccag tttcgacaca ttccaaaata atattttgac gcataaatct tcctccttca
    10801 atacttatcg atcttcaaga tatgcttgaa tttgttgtgc atcttcaaaa gttaactcgc
    10861 cagattccaa ttgtgaaact aaattttctt tcatatcaac aggtatctcc caataaccat
    10921 cgatacattg aaataactcc tcgtcatact cttcaaaaat tggatagatt cttaaaaaat
    10981 catcaaatga taagccttgg tcctcatagt aatctttgac taatccattt tcaattgcct
    11041 gttgggtcgg taaacccgtt tcagtaataa ttcctctctc taccatcgaa cgataaaaag
    11101 tgcatttatc tagcgattgc tgggtcgttt caagataata ctttaatcct tcttcaacta
    11161 aaggtgggta aataatttcc attttgattc cctcctaaaa gttatatatt aaactatttt
    11221 gaagctcctt taattacttt ctttccatta tagtaccctt ttaaagatac atgatggcta
    11281 cgtctataat caccattcgc ttcgtcaaat gaaatttctg gttttactac agtttgatga
    11341 gttcttctta accgtttctt cgccttagat gtttttcttg ctggtactgc catgatattt
    11401 cctcctttta ccaacttgct ttttttactc caggaatcaa cccttgatgg gcaagttctc
    11461 taaacttaat acgggacata ccgaactttc gcatataccc tcttggacga ccatccgtct
    11521 gatcacgaag cctcagacga ttcggatttg aatctcttgg caatttagcg agagcggtgc
    11581 gatctcctgc ggcttttaat tcctgacgtt tatctgcata cttttcaatc atcgccattt
    11641 gtttctttgc tttagcaatt ttcgactttt ttgccatata cctttatact ccttttattt
    11701 tgatttagac acctcaaatc gtaatcgtta cgttttgaca ctgatacttt accaacaaga
    11761 gttgctcttg tcaacaataa ataacatgaa ataaaactca tatgaataga taactatttg
    11821 aaaacgaatt tatcggtaca tagagataat caaatcattt aaaagaatat gcagtttagg
    11881 ctctgaaaaa taagaattca cttttattag tccaatcaaa cacaaatacc gttttaagta
    11941 gcttttcctc tacttaaaac ggcaatatga aagaaactat aactttttat tcaatctttt
    12001 atgtttaact ggtttatcgc caatttttta aaactttgat tattccttat attataagaa
    12061 tagacattaa gtgaactaat tcaatcatac tgattctttc gtgtattcaa aattagatgc
    12121 tgtttaataa aattattctt tttgaaaaag aattataact atatcccaaa aattgtaggt
    12181 taatttaaag cctatttcat catcgtgatt gagcttcttt ttcatagacg aatttctcag
    12241 atagtttagt tttctttatg agatttttgt atatttctga ccttcgatac aactcattat
    12301 gagtaccttg atcaactagt tttccttgat ccatcactac aattttattt accttttcaa
    12361 tagacttcaa gcgatgcgat ataattagta ctgtcttatc ttgtattaaa cgatttaaac
    12421 tttcctgaat acgtttttca ttttccgcat ctaaactggc agtaatttca tctaaaatta
    12481 aaattggcgc atttttcaaa aaagcccgtg ctatggatag gcgttgtcgc tctcctccag
    12541 aaagtgaact tccgttttct ccgaccaatg tatgataacc gtcaggtagt ttttcaataa
    12601 agtcttcgca gttagctaaa cgagctgctt ttttcacttc ctcatctgac gcttgactat
    12661 ttccaatacg tatattttcc aaaatacttg tattaaacaa agttacctct tgaaaaacaa
    12721 tagcgatttt gctaaacaaa gaagctggag atacacgttt aatatcatac ccatcaatta
    12781 aaatacagcc ttcatcataa tcatacagtc tagacactaa tttcaagata ctagttttcc
    12841 cggaaccact agcacctact aacgcagtca cttctccttg tttagccgta aaagagatat
    12901 gatctaaaat tggggtattg ttatcatatg agaaagagac atctcgcaac tcaatgtcaa
    12961 aagatttcag aggactatct gatccctctt ggatactggt ttccttcata gctctaattc
    13021 gctgtatttt aggagctaaa taaaatattt caagcactgc ttctttcata gaatcgaagg
    13081 aatcctttat ttttattgcg gctagtaagt aaccaacgac gtagagtata gtcacttcac
    13141 ccgttaacag taagtgaaca cctactagaa tgactactgc taaactaaca tatgagaaaa
    13201 tagaggataa ggccatgact gaaaaagtac ttaattctac ctttaaatga atcctttcac
    13261 tctcttccat ttttttgaaa agtctgtctt gaactttttt agatagatta aagctattaa
    13321 tctcttgctg caattctata gtttcttgaa attcttccga gttttctctc aaagtatcgt
    13381 aatatttagt attggctttc gtttgggatt tctttgataa caagattaag acaaaactaa
    13441 ataacgttgg caaaataaca gctaatccca ttttgacatt accaaccaaa agcatcactg
    13501 aaataaaagg gaaaaacaga gccataccgc cggctttagg tattgcatga ctcatcgcat
    13561 gctcaatacc ttcaacgtca gacatgatag tttgagataa atctgataaa ttatgttttg
    13621 aaaaatagga taatggtaaa tttgacaaat cgtctgcaat ttgcactctt aaatgcgcac
    13681 tttctttata agtactatta tataagtttt catactccct atctaacaac caatacatga
    13741 aaagcaaagt aacaaacgat acggcaaaat aaagccaacg gggctttgta ttttctaaaa
    13801 ctaattcgtc tatcagcatc attataatga atgctggtac catattaatg cagtatacaa
    13861 aaaaactaga aatagaagct ttagttaaag cttctgcgcc ctgatcactt aaagcaaatt
    13921 tgtttttata aaaacttttc attgtcaagc ctccaatcat ttgcattctc ataattagtg
    13981 accaattgtt tatacacttg atgtttagcc attaattcat tatgatttcc acgttctact
    14041 attttccctt ctgacaagac aagaatttca tctacagatt taatgctgga taatcgatga
    14101 gctatcataa taactgtttt ttctttcatc agatgttgaa aagccttttg tagttcatac
    14161 tcattatctg gatcaataga tgcactggct tcatccataa taacaatctt tgcatctttt
    14221 aaaattgctc tggcaatagc tatacgttgt ttttctcctc ctgacagata aactccttta
    14281 ctaccaatta aggtatgctc tctttcggga aatttttcta aaatcgattc gcaaccggct
    14341 aatcttagcg cgtcaaatac tttacttgct ggcgcttgag gatttgctat agaaacgtta
    14401 tcgtaaatac tctgattgaa tagtttcgta tcttgaaaga caaaagcaat tgtgttaata
    14461 attgtgtctt ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg tcccttatcc
    14521 acattgtaga aacctgaaat tagtttagct atcgtactct ttccgcttcc tgattttcct
    14581 actaatgcat aaattctttt ttgttctaaa actaagttga aattttctaa aacacactct
    14641 tctttgtatg aaaagctgac attttcaaat gcaatgtcac aatttttaat ctgtgtctcg
    14701 ttcccatatt ttagttggtc ttgcttcatt tctaaataaa gggagtctag tgtttcgact
    14761 gcatatcttg cttcaaacaa gtgcatactt acatacatta ttctcatgaa agaaacaaaa
    14821 ataattccac ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt gggagtggta
    14881 agagaagtta taaaaaaggt tataggaact atcaaaatgc aaactatgcc aaaaaatagc
    14941 cattgaaaaa gaacaaaagg tgttttccca ctcaaagaat acttatatgc aaacttagca
    15001 taatcgttaa ttgctttatt caaggcttta aatgatgtta ctttagcacc aaatatcttt
    15061 ataacttgaa ttccgcggat atattctacc gtttcagagc ttaacacatc taacgattct
    15121 tgatattttt gaataaaatt tgtattctct gtcattttct ttaacaagaa tacaccaatt
    15181 actaacatgg cgattagaac caatccaatt ttaaagttaa tgaaaaagcc taaggcaact
    15241 gataaaacag gtgttactat ggcttgagca ctatctggaa tcaagtgagc aattgccata
    15301 tgtgttttag ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga atttaaatca
    15361 aaaaaacgaa aacttgcttc tgttaaaccg ctaattccct tttttctcaa attagtctct
    15421 aagcgaaacc caaggatatg agaaaataag ccagaaaaaa tataaaataa agctctaaag
    15481 ttaaccaagc aacagtttga accgcataac tcttagctaa atattcatct cctacaataa
    15541 ttagagactg aagaaatttg taaatactgt aatatccata gactgttaat acgacagaaa
    15601 aatcagaaaa taaagtggct aaccatccgt agtatttcat tttagggaca taactaaata
    15661 attttttgta tactttcatt ttttcctccc ccgcattcaa tttcttctct tattcgttta
    15721 caactgttaa tgctttccgt aatagcgtaa attagagatg tacaaacaga taaaaggtaa
    15781 actacgttct attttttgta tttttttaag acttttcttc caatcaatac accaatcata
    15841 gataaacttg ctacaactaa cactgaaatg attccccata tacctaccgc atagtggatc
    15901 attgcatcca ttgttgcagc agattgtcct ttatctatcc aatcttgacg aaaacgatct
    15961 gtaaagaacc atataggaat ccactgtccg gcagcactgc ctaattgcat tagagcccaa
    16021 gacgcaactt gaggaattgc tcgatcatat ccaaatacag atagaataat atcagctaag
    16081 atcgccatac taatcgaact gataaaccaa ggtaagtacg ccccgcccat taacgtaaaa
    16141 aataacaata aaacaattgt gtaaatagta aaaattcctt ttctagggta gttatagcta
    16201 atataaacaa atatgattcc agataataat ccaaaaatcc cgggggatat gctgtgacca
    16261 aacgccccac caatactact aaccattatt gaaaaaaagt aaattactag ggaaataact
    16321 gtagctaaac caataaaaat ataatctttt gttttcatat atattctcct caattctatt
    16381 tcgttatgtc attaaaaatc tgttctatca aagttgaatc ttctactcta agttttatat
    16441 ctttttttat tccaccattt tttaaataaa taattctatt gcatgtttta taaagaaatt
    16501 ccggatcatg agtagctata actactgcac ccttttcttt taagtcttta ataaggtcag
    16561 caatcctttc catattaata taatccaacc cacttgtagg ttcatcaaat agaaataaat
    16621 tagtttttga caatgaagct atagaaacca ataatctttg tttttctcca ccggataaat
    16681 caaacggtaa ttgatctttc attttataaa gttccatttt ttttaaaatt ttttcttgct
    16741 gatctttact aactatttca ttccccaggg ctaactcgct cctcactgat tctgtgaaaa
    16801 attggtaatc catttcttgc attactaaaa acggcgatga caaatttttt ttattttcga
    16861 ttatctttcc tttggtaggg cgaataatct tcatgagtat tcttagtaag gttgttttcc
    16921 ccacaccgtt tttccctacc aaaccaataa catctccaac tttgacatca aaatttatgt
    16981 cttttagaat attttcataa gtaatttctt caacttttaa tacatctgca cttacctttt
    17041 cgatataggg aacttcaatc gaactattaa aaatgtcaaa tttccttaat gcgttattgt
    17101 tttcagtttt taacaactga atagttgtag tttttacagt tctattttta acaaatagaa
    17161 cttgatcaat taattctttc aaatagtaaa aacgatgttc agctataaca atagtaaatc
    17221 ctttattttt taaatcacaa agtaagtcag caagtttctt agtcgctttg aagtccaaat
    17281 ttgaagacgg ttcatccaat ataattaatt tagagtttag cgataaacta catccaatag
    17341 aaattttctg ccgttcacca cttgatagcg aaaaaatgtt tttatctaat atatttgaaa
    17401 gatctaataa ttcagctaat tcattgattc ttttatccat atgctctttt gtaaatccaa
    17461 agttttccat cgaaaatact aactcactcg ttgtatttgt tgtaaagaat tgtcctctag
    17521 gatcttgact tacgtagcca atattttttg atatactttc ttgtgttaag gattgtagcg
    17581 agttattttc aaataaaatt tccccaacca attttccgtc ataaagtctt ggaataagtc
    17641 cattcattaa ctttaatagt gtggatttcc cactcccaga agaaccagta atcaatgtaa
    17701 gactgccttt ttttattgaa aaattaaggt caacaagcgc ttgttcctta acatccttat
    17761 atgaaaaaga aacattgtta aattcaaaca attctttcac ctcaaatcaa aatacttaag
    17821 aaaaaaatag taacggaaaa caggatgata tagtctaata agctcatgtt tgttttatag
    17881 taagaacttc ttttcacagg agattctatc ccctttgtta ttgcattagc ggtcatttct
    17941 tcagcaatca tagttgcacg tataagaacg ggaactaaca gaaattccaa atattgcaga
    18001 ggctgaaaaa aagacaagcg aatgtttctc atttttaaag attctttaat cattcttatt
    18061 tcagaacgat aagtcggaaa gaatctgatc actactgtaa tacttaacat tatttttttc
    18121 ggtaaatgta ttttttctaa agaggataat aattcattgc tagtatagac actaaccaac
    18181 aaccatgaga tcattacgac cggcaacatt cgaaatacaa tatataagga tgtcgcaaaa
    18241 aatatagtaa aaaactctat cttgatagca tcaataattt tcattactaa aaacagaact
    18301 gaataaatca agatgaaata aatacatgtc tttattttac gaaacatcaa tactaaaact
    18361 gtagcaataa tgaaacaccc cacttgaaat atatttccct ttcctaaaaa aataatgacc
    18421 ggaaaaaaaa tatttaaagt aatcaaggtt aacggattga tttttgacac tctttcacct
    18481 ccaaaaaata agatcgcact gttatcttaa gctgataaaa atgcaccgtt attcagataa
    18541 cagtgcgatc ttatcaaaaa gcataactga gaacaattct caatgcaagc tttttttaat
    18601 taactcagta attcaatcca cccaggaaca gaaaattgat acaatcttct ggttaatagc
    18661 atagtctttt cttttgaata gctatgcttc aatatatcat agaatgtagc gtagaatgag
    18721 taaatgtaga agtggatttc ttcttcatta aagactaaat tttgattgat cattccgata
    18781 gtctttaact tatctagaca acagactgtc tttttcgtca caaatgatgt aattttttca
    18841 acaatatctt gatatttggt tccagctgat tggaataaaa tcaaatcgaa tatacgttta
    18901 ttctcaaaaa tataattaat aaatggatct aattcattga agttctctct ccaacttttc
    18961 tctatatcta tattttctaa gtttaagagg tatttggtaa actcttcatt atacatatta
    19021 ataagcgcat taatatgcgg ctgaataatt tcttcaaata acgcttcctt agaagtaaaa
    19081 tgtttataaa aagcacccgt agttacgcct gcggtcaggc acagttgcct tatcttagtt
    19141 tgctgaaatc cttctttttc aaaatggctt agtccacttt ctattaactt ttggtgagtt
    19201 ataatataat ctctggtttc tatgggtact tcctcctaat aagatgaatt aactttatta
    19261 tcttacacag gacttgaagt ttcaatcgtt aacgtttatt actgaactca ttccttagag
    19321 ctaaactaat gagaaagagc agaataactg atacaaaaag caatcctaca agaattaata
    19381 gactcatcta aatcattcac cttttctaaa ataatccgag aaagatttaa aaaaattata
    19441 tccagctatt aggataagtc ctatcataat cactccaata gccattttct tatcctccct
    19501 aaaaataaag tcttttactt aaaacctttt ttgtaatcgt caataaattt tttcccttta
    19561 tcagttaatt gaaatcctat atctaaatag tcctcatatc taagtttagc cagcatctca
    19621 cttacagatg ctggactaac acttagaact tttgctaaat cattattttt taatttcttt
    19681 tttctttttg ataattcctg aattgctagt aaataaatta gtatagtagt cgttttaatt
    19741 tgtaccatac ctaccctcca aattatttcg aaaggccttc agcaatttta tccaggttcc
    19801 atttcatcat cgcatagtaa ctatcaccat cttgtccttt ttctgcaatt gaatcagtaa
    19861 aaatcgttga atagatagga atattggtat cttttgatac tgttttcatt ggtctatcat
    19921 ccacactgct ttctacgaat aaggagggaa cttttgttgt gcgtaatttt tcaactaagt
    19981 gttttatttg atctggtgtt ccttcttctt cagtgttgat ttcccaaatg taagcagaag
    20041 gcacattata cgctttcgag aaatatttaa agcatccttc acttgtaaca atcatttttt
    20101 tatcattcgg aattgaagca aatttagatt tagcttcttt gtctagagaa tccaattttt
    20161 caatatactt atctagattt tctttataga attttttatt atcaggatct ttttccgcta
    20221 actgtttttc aatattttta gcgtaaataa taccgttttc taaatttaac caagcatgag
    20281 gatcttcctt ccctttctca ctctgaccct ctaagtaaat aacatctatg ccatcacttg
    20341 ctgcaaaata gtctttgttt tcctctttgt tcgcattttt aactaatttt gtaaaccaag
    20401 catttcctcc agtttccaag ttaacaccgt tataaaaaat caaatctgct tttgaagttt
    20461 tttgaacatc ttcaggcaaa ggttcatatt cgtggggatc tttcccaata ggtacgatac
    20521 tgtgtaaatc aattttatct tttgctatat tttcggtaat atctgctaaa atcgaattag
    20581 taactactac ttttaatttt tcgttcgaag ccactttatc tttgtcttta ctatttattg
    20641 aacatgcccc gaatataaaa agagcaatca aaatccccag acttcccagt actgctttcc
    20701 attttctcat cataacgatc tctccttctt tcttaaaaat ttttgttttg gtgagattag
    20761 aaaactaatt acaaataaga atgccgctgt taaaacaata cttgatcctg ctgctatgtt
    20821 aaaactataa ccaataaaca atccaagcaa tgatgaactt gctccgactg ttgcagaaat
    20881 aaatatcata gttttcaagc tatttgcata taaatatgct gtagctgctg gtgtaattaa
    20941 cattgcaaca attaatatag ttcccacact ttgcatagct gttactgcta ctaaagttaa
    21001 tagaaccatc aatagataat gataaaaact gacattcatc cctattgctt ttgccattaa
    21061 tggatcaaaa gaagtgatta ataattcttt aaaaaataga actactataa ccaacactaa
    21121 aaccgaaacg ccaattgtaa tccacttatc aatatcttgt acagctaaaa tatttccaaa
    21181 taaaatatga aataaatccg ttgagctatt tgcaactcct ataaggataa ctcctagtgc
    21241 taaaaaagaa ctgaaagtaa taccaattgc tgtatctcct tttattacac tattgctttt
    21301 tataaaagta ataatgatag aagctaatag accaaataca atcgcaccaa taaaaaaatt
    21361 aatacctaaa atataagaaa ttgctactcc tggcaaaaca gcatgagaaa tagcatcacc
    21421 cataagagac attcctctta aaataataaa acaacctact gttccagaaa caatccctat
    21481 aactacagat gtaattagag cattctgtaa aaagtgaaag tcatacagac cattaataaa
    21541 attttgaagc atattattcc ccctctccaa taaatatagt atctccatag gcttttttta
    21601 aattttcttc attaaataca gattctacac ttccgtgagc aattagattt cgattcaaaa
    21661 gaataatatt atcaaaatac ttctttacct tgcttaaatc atgatgcaca attagcactg
    21721 tttttccctg atgtttcaat ttttttaacg tatccataat tattcgttca ctaactaagt
    21781 ctatccctac aaatggttca tctaaaaata tgaaatccgc attttgagca agacatcttg
    21841 ctaaaagtac acgttgaaat tgtcctccag ataattctcc tatttgatga ttagaatatt
    21901 ttcccatatc gaccttttcc aaagcctttg aaactctttg ccattctgca tttttaattc
    21961 tttgaaaaac tttcattccg gcatatgttc ccatagatac acattctttt acttttattg
    22021 gaaaagtaaa atcaatatca gctttttgct ctacatatgc aatcttcacc agttttttat
    22081 tgatatcttc attattaatg gagacctctc cctgatgagg gataatattt aacattgctt
    22141 taagcaatgt ggattttcca gcaccatttg gaccaataat acctgttaaa gcaccttctt
    22201 gtatttttaa agaaatctcg ttcaatgcta aaaaatcgtt ataacaaaca ttaagctttt
    22261 tcacttctaa cataactttc tccttctaaa tgata
//
LOCUS       DARBFD010000043        22031 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000043 DARBFD010000000
VERSION     DARBFD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.043"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            367..555
                     /locus_tag="SCK08_001522"
     CDS             367..555
                     /locus_tag="SCK08_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201404.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="SCK08_001523"
     CDS             545..805
                     /locus_tag="SCK08_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201405.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="SCK08_001524"
     CDS             846..1073
                     /locus_tag="SCK08_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201406.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="SCK08_001525"
     CDS             1138..1710
                     /locus_tag="SCK08_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201407.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="SCK08_001526"
     CDS             1711..2022
                     /locus_tag="SCK08_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1201408.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="SCK08_001527"
     CDS             2013..2273
                     /locus_tag="SCK08_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201409.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="SCK08_001528"
     CDS             2434..2793
                     /locus_tag="SCK08_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201410.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="SCK08_001529"
     CDS             2790..4433
                     /locus_tag="SCK08_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1201411.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="SCK08_001530"
     CDS             4468..5550
                     /locus_tag="SCK08_001530"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201412.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="SCK08_001531"
     CDS             5594..6766
                     /locus_tag="SCK08_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1201413.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="SCK08_001532"
     CDS             6753..7457
                     /locus_tag="SCK08_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG1201414.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="SCK08_001533"
     CDS             7462..8622
                     /locus_tag="SCK08_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1201415.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="SCK08_001534"
     CDS             8698..8976
                     /locus_tag="SCK08_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG1201416.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="SCK08_001535"
     CDS             8957..9328
                     /locus_tag="SCK08_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEG1201417.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="SCK08_001536"
     CDS             9344..9748
                     /locus_tag="SCK08_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201418.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="SCK08_001537"
     CDS             9745..10155
                     /locus_tag="SCK08_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201419.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="SCK08_001538"
     CDS             10216..10803
                     /locus_tag="SCK08_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1201420.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="SCK08_001539"
     CDS             10876..11343
                     /locus_tag="SCK08_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEG1201421.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="SCK08_001540"
     CDS             11389..11697
                     /locus_tag="SCK08_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201422.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="SCK08_001541"
     CDS             11892..18626
                     /locus_tag="SCK08_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1201423.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="SCK08_001542"
     CDS             18630..19502
                     /locus_tag="SCK08_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEG1201424.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20548
                     /locus_tag="SCK08_001543"
     CDS             19499..20548
                     /locus_tag="SCK08_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201425.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="SCK08_001544"
     CDS             20552..21376
                     /locus_tag="SCK08_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201426.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="SCK08_001545"
     CDS             21394..>22031
                     /locus_tag="SCK08_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEG1201427.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DARBFD010000044        21988 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000044 DARBFD010000000
VERSION     DARBFD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.044"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SCK08_001546"
     CDS             <1..395
                     /locus_tag="SCK08_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201428.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="SCK08_001547"
     CDS             398..1147
                     /locus_tag="SCK08_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201429.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="SCK08_001548"
     CDS             1159..1428
                     /locus_tag="SCK08_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201430.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="SCK08_001549"
     CDS             1433..1597
                     /locus_tag="SCK08_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201431.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="SCK08_001550"
     CDS             1590..1892
                     /locus_tag="SCK08_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201432.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="SCK08_001551"
     CDS             1889..2050
                     /locus_tag="SCK08_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1201433.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="SCK08_001552"
     CDS             2047..2352
                     /locus_tag="SCK08_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201434.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="SCK08_001553"
     CDS             2352..2708
                     /locus_tag="SCK08_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEG1201435.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="SCK08_001554"
     CDS             2695..2913
                     /locus_tag="SCK08_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201436.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="SCK08_001555"
     CDS             2910..3329
                     /locus_tag="SCK08_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG1201437.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="SCK08_001556"
     CDS             3326..3883
                     /locus_tag="SCK08_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG1201438.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="SCK08_001557"
     CDS             3880..4176
                     /locus_tag="SCK08_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201439.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="SCK08_001558"
     CDS             4253..4666
                     /locus_tag="SCK08_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG1201440.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="SCK08_001559"
     CDS             5012..5386
                     /locus_tag="SCK08_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201441.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="SCK08_001560"
     CDS             5423..5590
                     /locus_tag="SCK08_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201442.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="SCK08_001561"
     CDS             5616..5960
                     /locus_tag="SCK08_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1201443.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="SCK08_001562"
     CDS             5965..6246
                     /locus_tag="SCK08_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201444.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="SCK08_001563"
     CDS             6349..6663
                     /locus_tag="SCK08_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEG1201445.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="SCK08_001564"
     CDS             6641..8335
                     /locus_tag="SCK08_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1201446.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="SCK08_001565"
     CDS             8355..9533
                     /locus_tag="SCK08_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1201447.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="SCK08_001566"
     CDS             9496..10182
                     /locus_tag="SCK08_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG1201448.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="SCK08_001567"
     CDS             10182..11342
                     /locus_tag="SCK08_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1201449.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="SCK08_001568"
     CDS             11352..12227
                     /locus_tag="SCK08_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201450.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="SCK08_001569"
     CDS             12224..12535
                     /locus_tag="SCK08_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201451.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="SCK08_001570"
     CDS             12525..12878
                     /locus_tag="SCK08_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG1201452.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="SCK08_001571"
     CDS             12868..13269
                     /locus_tag="SCK08_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201453.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="SCK08_001572"
     CDS             13262..13666
                     /locus_tag="SCK08_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201454.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="SCK08_001573"
     CDS             13678..14286
                     /locus_tag="SCK08_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1201455.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="SCK08_001574"
     CDS             14306..14668
                     /locus_tag="SCK08_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201456.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="SCK08_001575"
     CDS             14671..14853
                     /locus_tag="SCK08_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201457.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="SCK08_001576"
     CDS             14870..18289
                     /locus_tag="SCK08_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1201458.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="SCK08_001577"
     CDS             18340..19077
                     /locus_tag="SCK08_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1201459.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="SCK08_001578"
     CDS             19087..21378
                     /locus_tag="SCK08_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1201460.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="SCK08_001579"
     CDS             21402..>21988
                     /locus_tag="SCK08_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201461.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DARBFD010000045        21911 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000045 DARBFD010000000
VERSION     DARBFD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.045"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCK08_001580"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCK08_001580"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEG1201462.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="SCK08_001581"
     CDS             complement(1172..1867)
                     /locus_tag="SCK08_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEG1201463.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="SCK08_001582"
     CDS             complement(1880..2596)
                     /locus_tag="SCK08_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEG1201464.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="SCK08_001583"
     CDS             2926..3534
                     /locus_tag="SCK08_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEG1201465.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="SCK08_001584"
     CDS             3859..4182
                     /locus_tag="SCK08_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEG1201466.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="SCK08_001585"
     CDS             4364..5734
                     /locus_tag="SCK08_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEG1201467.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="SCK08_001586"
     CDS             complement(5864..7165)
                     /locus_tag="SCK08_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1201468.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="SCK08_001587"
     CDS             complement(7519..9477)
                     /locus_tag="SCK08_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEG1201469.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="SCK08_001588"
     CDS             complement(9654..9920)
                     /locus_tag="SCK08_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1201470.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCK08_001589"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCK08_001589"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEG1201471.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCK08_001590"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCK08_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEG1201472.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="SCK08_001591"
     CDS             13264..13827
                     /locus_tag="SCK08_001591"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1201473.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="SCK08_001592"
     CDS             complement(14080..14601)
                     /locus_tag="SCK08_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEG1201474.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="SCK08_001593"
     CDS             complement(14604..15446)
                     /locus_tag="SCK08_001593"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEG1201475.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="SCK08_001594"
     CDS             complement(15439..16164)
                     /locus_tag="SCK08_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEG1201476.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="SCK08_001595"
     CDS             16340..16636
                     /locus_tag="SCK08_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201477.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="SCK08_001596"
     CDS             17204..18796
                     /locus_tag="SCK08_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1201478.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="SCK08_001597"
     CDS             18809..19549
                     /locus_tag="SCK08_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1201479.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="SCK08_001598"
     CDS             19717..21066
                     /locus_tag="SCK08_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1201480.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="SCK08_001599"
     CDS             complement(21194..21784)
                     /locus_tag="SCK08_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1201481.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6294 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa a
//
LOCUS       DARBFD010000046        21563 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000046 DARBFD010000000
VERSION     DARBFD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.046"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            244..945
                     /locus_tag="SCK08_001600"
     CDS             244..945
                     /locus_tag="SCK08_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1201482.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1554..2570)
                     /gene="tsaD"
                     /locus_tag="SCK08_001601"
     CDS             complement(1554..2570)
                     /gene="tsaD"
                     /locus_tag="SCK08_001601"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEG1201483.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2567..3031)
                     /gene="rimI"
                     /locus_tag="SCK08_001602"
     CDS             complement(2567..3031)
                     /gene="rimI"
                     /locus_tag="SCK08_001602"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1201484.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3038..3580)
                     /gene="rimI"
                     /locus_tag="SCK08_001603"
     CDS             complement(3038..3580)
                     /gene="rimI"
                     /locus_tag="SCK08_001603"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1201485.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3564..4286)
                     /gene="tsaB"
                     /locus_tag="SCK08_001604"
     CDS             complement(3564..4286)
                     /gene="tsaB"
                     /locus_tag="SCK08_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEG1201486.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4477..5457)
                     /locus_tag="SCK08_001605"
     CDS             complement(4477..5457)
                     /locus_tag="SCK08_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1201487.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5481..6965)
                     /gene="galT"
                     /locus_tag="SCK08_001606"
     CDS             complement(5481..6965)
                     /gene="galT"
                     /locus_tag="SCK08_001606"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEG1201488.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6977..7966)
                     /gene="galE"
                     /locus_tag="SCK08_001607"
     CDS             complement(6977..7966)
                     /gene="galE"
                     /locus_tag="SCK08_001607"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEG1201489.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8214..8381
                     /locus_tag="SCK08_001608"
     CDS             8214..8381
                     /locus_tag="SCK08_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEG1201490.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8443..10254)
                     /locus_tag="SCK08_001609"
     CDS             complement(8443..10254)
                     /locus_tag="SCK08_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEG1201491.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10251..10616)
                     /locus_tag="SCK08_001610"
     CDS             complement(10251..10616)
                     /locus_tag="SCK08_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEG1201492.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10779..11174)
                     /locus_tag="SCK08_001611"
     CDS             complement(10779..11174)
                     /locus_tag="SCK08_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1201493.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11192..12154)
                     /locus_tag="SCK08_001612"
     CDS             complement(11192..12154)
                     /locus_tag="SCK08_001612"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEG1201494.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12154..12366)
                     /locus_tag="SCK08_001613"
     CDS             complement(12154..12366)
                     /locus_tag="SCK08_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEG1201495.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12387..13085)
                     /locus_tag="SCK08_001614"
     CDS             complement(12387..13085)
                     /locus_tag="SCK08_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEG1201496.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13105..13647)
                     /locus_tag="SCK08_001615"
     CDS             complement(13105..13647)
                     /locus_tag="SCK08_001615"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEG1201497.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13779..14783)
                     /locus_tag="SCK08_001616"
     CDS             complement(13779..14783)
                     /locus_tag="SCK08_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1201498.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14780..15769)
                     /locus_tag="SCK08_001617"
     CDS             complement(14780..15769)
                     /locus_tag="SCK08_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1201499.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15766..16572)
                     /locus_tag="SCK08_001618"
     CDS             complement(15766..16572)
                     /locus_tag="SCK08_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201500.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16738..17694
                     /locus_tag="SCK08_001619"
     CDS             16738..17694
                     /locus_tag="SCK08_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1201501.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17771..18289)
                     /locus_tag="SCK08_001620"
     CDS             complement(17771..18289)
                     /locus_tag="SCK08_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1201502.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18377..18526)
                     /gene="rpmG"
                     /locus_tag="SCK08_001621"
     CDS             complement(18377..18526)
                     /gene="rpmG"
                     /locus_tag="SCK08_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1201503.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18754..19200
                     /locus_tag="SCK08_001622"
     CDS             18754..19200
                     /locus_tag="SCK08_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEG1201504.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19395..21290)
                     /gene="asnB"
                     /locus_tag="SCK08_001623"
     CDS             complement(19395..21290)
                     /gene="asnB"
                     /locus_tag="SCK08_001623"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEG1201505.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6497 a         4564 c         3708 g         6794 t
ORIGIN      
        1 aaattaaaaa aataaattat attatcgttg ttagcgttta ctatatgtat tattgttgtt
       61 ttttatacac aaatcattcg cttcaaaaca aattattcaa taccattgta tattttttac
      121 ataataacaa attatatttg taaagtatta aaacttgttt ctaatgttgg tatgttaaaa
      181 ctttacaaat tctttaaaaa aagtgacaat aaatatagtg aacaataagg agaggacttt
      241 ttaatgcgtg ttaataaaat cgattttcac ttttcaaaac tcagatatca acctgatttt
      301 gaacatttct cggatgaaga gttccaacag atcaaggaac atactgttct tcgttcttat
      361 aaaaaaggac agatcctttt cgacgatggt gatcccagac aacggttgta tatcttgacc
      421 aaaggactcg ttcgtttaga acgttatgat gaaagtgcaa cttattacta ctatgactat
      481 gtgaatacaa ataacctttt tcctatgtca ggaatgtttg gagaagagac ctacacttat
      541 acagctcaag cgatgactga tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa
      601 ttagcacgaa aaaatcccga acagttgatc tatatgatac aaaaaatttc taaagtagtt
      661 gaaatacatg aattgcgtat ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa
      721 caaaatttat tcattttgaa agaagaattg ggcgtaccaa caaaagatgg tcatatcacc
      781 attccttatc cgatcacctt aaaagaatta gcagtgaata gcggcacgac acgtgaaact
      841 gctggacaag taatcaaaca actacgtgat tccgggctgt tagattatga caaaaagcgt
      901 tttactttct gtaagacagc tactactcca gctatgagag aataacgcga acaaaaataa
      961 gcagtgaccc tgataaatat cagggtcact gcttattttc ctccctaaaa cttctgaaag
     1021 atccggcagt tttagatcac tggacaagac tcaaagattt tctatgtgtg tttcagttat
     1081 gtaatattca ctgtttgcaa aaaatactgt tagattaact agatgaagcg tcttgtcctc
     1141 atccgatcta gattattcga tattcctcga caataaacga ttactcagtg agggcaatca
     1201 gaggaacgga taatgataaa taagctgtaa aaaatagcaa aataacaata atgattaaaa
     1261 gtatgtaaaa aatagggctt tgacaaaaac gtgtcacagc cctatttcga ttaaacggtg
     1321 tgtacctgtc aactcttcgg tcacaagtca taatgttcat aaacatgagg agctgttttt
     1381 tgaaattgtt ccttgtgaat cagagttaaa cgacaggctt acgaactctt gattaaacgg
     1441 cgttcaaaag cttgcatacg cggtaataag aacagccagg tcaaaaacat gagaagccgt
     1501 ttttcctggg ctgttcttat tacttgatgc agggcagctt tttcacaacc tctttattca
     1561 gataaaacca agcttggatc agcattcaac tgatagctag caaaatttct tttttgcatt
     1621 tccacatgtg ctgctgctcc aatcattgca gcattatctc cacaaagtcg aagaggggga
     1681 atcactagtt ctacctcagg gagtttagct gccaaagcat cctgcaatcc ttcacgcaac
     1741 ccttgattgg ccgctacacc tccagctata atcagttgct tgactggata attttgacaa
     1801 gctcgaagtg ttttagtaat caatacatcg atcacacttg cttgaaagct agctgctaaa
     1861 tcatttttgt ctagatcttc tccacgctgt tgtgcattat gcaccaaatt gataaatgca
     1921 ctttttaatc cgctaaaact gaaatcataa ttatcttcat ggatcatcgc tcgcggaaaa
     1981 tggtaattat cttttccctg atgagctagc tgatcgattt cttttccgct tgggtaagaa
     2041 agacctaata ctcttccaac tttgtcataa gcttctcctg ctgcgtcatc tcttgtctcc
     2101 ccaatgattt catatgatcc atcttcttgc atatagacta attctgtatg tcctccgcta
     2161 acaagaagtg ccatcaatgg aaactggaat ggtttaacca gacgggctgc atagatatgc
     2221 cctgccatat ggtttacagg aataagcggc aattggtgtg cccaagcaaa agccttagct
     2281 gcagaaatac caatcagcaa cgagccgaca agtcctggtc cataagtgac agcgactgcc
     2341 gataaatctt ctgccgacac accagcttct accagtgcat cttccaagca taaggtgatt
     2401 tgttctacgt gatggcggct agctacttca ggtactacgc caccaaaacg tttatgactg
     2461 tttatttgag aagcaacgat gttagataag atctctgttc catttctaac gaccgctaca
     2521 ctcgtttcat cacaacttga ttcaatcgcc agaatcaatt cttcattcat ttcatcgtct
     2581 ccgtttttaa ctttctgctc ataatgatcg catcttccgt cggattgcga taatatgatt
     2641 tccgtttacc taatatacga aacttttctg attcataaag ttttcttgct gcttcattag
     2701 atagacgtac ttctaaaaaa acatgcagcg aatcttttat ttgttccgta atcagtaatt
     2761 gtaacaaatg ccgtgcatgc ccttttcctt gttctgaaac tgcaacagca atatttgtga
     2821 tctcgacttc atctattact ttcgagtatc caataaagcc acagatctgg ttatcttcag
     2881 ctaaaagcag ataatcagag tgaggctgtt ggatgtctgc caaaaactgc attttcgtcc
     2941 aaggtgaacc atgtggataa gcattttcgc taatcatcca tacgcattct gccaagtacg
     3001 cttcatcaaa atcttttttt ctgcaaatca actaatctca ctcattttct aaaagtttta
     3061 tcatatccaa cgcatcttct tttgtttcag tgtaatatct ttttttgatc gttgttgctt
     3121 gaaaccccaa tttccgataa agtctttgtg catcttgatt actcatacgc acttctaatg
     3181 acatcgtctc acagcgattc attatagcaa agttctcgat ttcgtcaata agcagcgaac
     3241 cgattcgttt acgctgatat gccggtcgaa cggcaatatt cgtgatatgg caatctaaac
     3301 cgaacgctct tgaaccgata aaaccaatca ttcttccatt atcaaagata cctagatata
     3361 gatgaggaag cggtgacttc aattctgata taaaggcact ttttgtccat ggcaattcgc
     3421 cattatagac ttctcgttcg acatccagca gctctttgat atcttctgta gtcaattctc
     3481 ttagtacgta acgacgatcg atttgttcga ctgttttttc tgtataaatc attttatctt
     3541 gaaaaaagct tctggcatac gctctaaatt ttttcaacat aactttcgtc tccaggtgtg
     3601 tgcgtctcca accatttttc ttctgcttct actcgtttta aatagcgagg aagaaaagct
     3661 tgtttctctg caactggttc aactaaagca cctaattgag ccacagttac accacttgga
     3721 atatcccatg ctggaactgt attaatcatc gcactaggca atatttcctt tatttcagag
     3781 aagaatttgt gacaatcact cccgacgaaa taaatagctt cattcagctt ttgtagttgt
     3841 gtgaacaaat cagaagcaga aatatgtaca tcttcaagta cagtttctaa tctattttct
     3901 ttccagcggt atgccccagc gtatacattt tttcttcgag catcaaacat gggcacaata
     3961 atattatcca caccgatgca attagccgcg atggttttca gactagatac tcctactagc
     4021 tctttatcta atgtataagc aatcgttttt gctgtcgtta cacctatacg aagccctgta
     4081 taagaaccgg gaccatcaga aaccgcgatt cggtcaatgt cttttggact tatttgaaga
     4141 tctgcaaaca attggtcgat tgcaggcatc agtgttacac tgtggttgcg tttgatattt
     4201 gtttgtattt gtccaagaat ttgttcgtct tctaccacac cgatcgccaa agtttggttt
     4261 gcagtatcta ttcctaatgt aatcatatat ttgatccttt ctgctatgac attctcgcct
     4321 tccattgtag catacttttt tattctgagg atgaaaatac caaaggcaaa actagtacaa
     4381 taatacatga ttatctgctg gcgggcaaat tttgcccctt cctgaaacac aaaaaagctg
     4441 cggtatcact ccattttccc acagcttttc catcttttat ttcgttgatt ctcgaataat
     4501 caactctgtt tccacaataa tctttcttga aaccggtgct ttggaattac ttaattgaag
     4561 aatcgtctcc actgctagct ctcccatcca ttcggtataa acttttacag tagaaagcga
     4621 gggcgttaca tatttcgcta cactgacatc attgaagccc atcacagaaa tatcttctgg
     4681 taccactaaa cctttttctt gaatcgcttt cagtgcgcca atcgctaatg catcattagc
     4741 tgcaaaaagc gcatcaggaa catgcggatt cgtttttaaa tattgcttca ctgcttgata
     4801 tcctgactcg acagaaaaat ctgcttcgat aattggacct tcgtcgtagc ctttttctgc
     4861 caaaacttgt tgaaatgcca taattctggg atcttttagc cgctgatggt ttttcttcgt
     4921 atattctgtt ccagcaagca tagcgatatg ttgcgcttta tgggagacaa agtgttcaat
     4981 gactttttga acacttgact taaagtcgac aactagagag tcgattcctt gagaagtgcc
     5041 gtcagaatct accaaaagca aggcattttg ggatcctttc atttctaata actgagcttc
     5101 gtcgaatttt cctagtgcga tcgttccatc tgccggaaga tcactaatgg aatcccacgt
     5161 ttctttcaat aaagtaacgt ttgcttcttc tgcctttttt tcgattccta ggcgaataga
     5221 taaataataa agatcttcaa gctcttccgt atcgtcgtac cactggatca ggcgaagcgt
     5281 ttgcttagta tttgctgcat ttttacgatg tttcgtataa tttaagcgtt ctgctgcttc
     5341 aaaaattttt tgctttgtgg ctaagccaac ggaaagatca ggatcatagt tgagaactct
     5401 tgaaacagta gcaggagaaa ctcctgccaa ctgcgcaatg tctttaattg ttgccatttg
     5461 tctctcctcc ttttttacgt ttataaaatc gcagtaaatc gttcaaacgc tgcctgtcct
     5521 ttttcatctc gcttgaatac tccagcgtct tccagtacac gtgcaaaaac ctgacctaca
     5581 gatttttgta cgacatcggt gaccgtatct ttcgtcaaat caggatgttc tttttttaat
     5641 tgttctgccc acggacgatg atattcagcc atctggtttt cttctcctaa taagaaatgc
     5701 tctacttcta ctagttctgg tttcaaacga ggtggtaaaa cagcaagccc cattacttcg
     5761 atcaacccaa tgttctcctt tttgatatgt tggacatctt tatgtggatg aaaaatccca
     5821 tcaggatgtt cttctgaaac attgttgtct cgaaggacaa ggtccaactc aaagtaattg
     5881 tcccttcttc gtgcaattgg cgtaatcgta tggtgtggtg taccatctgc tgaaaaagct
     5941 ctgatagata ctgtttcatc cgaatatgct ttccattttt ccaatactga gttagctgct
     6001 tcaataagca agtttttctc tgttgattgc aaacggatca cagacatcgg ccatttcacg
     6061 actcctgcga tgacttgcgg ataatcttct agtttgataa gtttttctat cggtgctttt
     6121 gccataggaa attcatgacg tcctgcctga taatgatcat gagaaagaat cgatcctcca
     6181 acaattggta agtcagcatt cgaaccaaca aagtaatgag gcaagacttc agtaatccga
     6241 agcagtctag caaatgtttc ctttgtgatt ttcatcgggc ggtgttgttc tgataaaatg
     6301 atcgaatgtt cattgtaata agcataaggg gaatattgga atccccagct ttcaccgtct
     6361 agattcattc gaataatccg gtgattggtg cgggctggat agttcaagcg acctctatat
     6421 ccttcgtttt ccatgcaaag catgcaggta ggatagtcta cttttaccgc ctcgcgttct
     6481 gctgcgattt gttttggatc tttttctggc ttagataaat tgattgtgat ttccagttca
     6541 ccatactccg actctgccgg aaatttgatg ttttttgcga tggctcttgt tttgatgtag
     6601 tcatttttct tacttaaatt aaagaaatac tcagttgctt cctctgggct tttcgaatag
     6661 tgctgtgcaa agaatgcatt gacgactgaa ggtggcggtg tgagaaaatc cattaattgc
     6721 gcttcaaaaa tctcttgttc tgctggcaat tctgaaagaa caccattttc tcttgcctta
     6781 gcgacaagct gatccaataa gtctacagat tcttccttaa ctggtcttac ttcaatgctt
     6841 tctagagcat cttctcctac catccctaaa agacgattct gtaaatataa gcggtccatt
     6901 tccatccatc cgccagcttc gatcgcaagt gtggcaaaat cacaaatcgt ttgactgatt
     6961 gacatatctc attcccctat tcttcataac catttggatg gctgacatgc caatcccatg
     7021 ctgttttgat aatcgctttg atatctgtgt attcaggttt ccagcccaga atctctctcg
     7081 ctttttcact tgaagcgact aatcggctag gatctccagc tcttcttggt gctatttctg
     7141 ctggaatttc ttttcctgtc acttctctag ctgcttctag catttccttg actgagtatc
     7201 ctttgctgct gcctaaattg aagaagttgc tttcatttcc ttctttcaag tattccaaag
     7261 ctaaaatatg tgcagcaatc aaatcttcta cttgaacata atctctgata catgttccat
     7321 ctggtgtatc ataatcgtct ccataaacag ccaaggcttt tctttgacca agagctactt
     7381 gtaaaatgat tgggacaaga tgcgtttctg gtgtatgatc ttctccgata gatgcatctg
     7441 cttttgctcc ggctacattg aagtagcgca aagcgacata gcgcatgcca tatgcttggt
     7501 cacaccactt catcattttt tccatcatca atttactttc accatacgga tttttaggat
     7561 tagctggtgt atcttctgtg attggagact ctttcggctc tccataagtt gctgcagtag
     7621 aagagaatac gattttattc acgttgaact cgtgcatgac ttcaagtaag atttgcatac
     7681 cgtatacgtt gttgttgaaa tacatcaatg gtttttcgac agattctcct actaaagagc
     7741 ttgcagcaaa atggattact ccttcaatcg gttccttttc aaatacggaa cgtagaaatt
     7801 ctttatcccg gatgtctcct tcataaaaat gagcatctgg atgtactgct tgtttgtgac
     7861 ctgtgagcaa attatctacc acagctactt gatacccctt ttgaactagc tggtctacag
     7921 cgtgcgaacc gatataaccg gctccgccta gtactaaaat tgtcataaaa aacactcctt
     7981 tgattagtaa aattgtgttt gttgattcta tataatttaa aaaacgaagt ttacttttat
     8041 ggtgaaaaca aacaaagtaa aatttcgtaa attcatatgg taaatttata ataaaacatt
     8101 tagtaaacat tttcaagggt tttttgcaaa ggtttacaaa caaacgtaaa actagccttt
     8161 ttttctcttt tgttccataa tagatataac aaataataaa tgaggtggat tcaatgaaaa
     8221 aatttgtatc tggaatatta gtaggaagtc tggcaacagc cgctgccgtt gctggtttgg
     8281 ttgcttctgt gaaaaaaaca gtgatcgatc caattgacga aaaagaagca atgatcgaag
     8341 aaaaccgtaa aaaagcaatg cgtaaacgcg tttctcgtta ataattattt aaaaatacgc
     8401 atgaagactt tctaaaaaac agataagtca tcatgcgtat ttttatttgt cagtcaatat
     8461 aagttgtgct attttatctg cctgtctcag attccatgga tctgtataag gtctagctac
     8521 tgctgcagtt agccaatcca caccttcaac aggtattcca tagcaataaa agtcagactg
     8581 gatttcccct gaagcatcaa tcacgcgatt cgtacctcgc tcaacatcta tagcttctga
     8641 atgataactt cctgtattat ctgaaaatga aaaagggcga acaagtttgt tttccttcaa
     8701 attttttatg acaggattac ttgaacgaga aaagcttgtt tgtggcaatc gagcttcaat
     8761 caaatagcga ctgctgattt ctttatcttg tagagaaata acatagcggt tgtgcttttg
     8821 ctgaatatca aatggcggtt cgatcaaatg aaaaataccc gcatctatca gtgctgctaa
     8881 ttctttcgtc cgaatgattg gcggaccgat cgttaaaaag ctatttagat gagtaaacca
     8941 tccccatagt atttctttgt attccttagc agaaaaaacg tgccattcaa tagcctgccg
     9001 gattggatca cgccagtctt ttaatgcatc aaatgttgaa gcaaatgctc ctgtgcaatt
     9061 gcctttttcc gcttctttta tctgcccttc aataaatatt cggctagctt ctgaaaaggg
     9121 ctcattctga taggcaagag gagtttctaa atacgaccaa tcccatctat aagggattag
     9181 tgctggatgc tttccgagac actcctctga agagagggag agaaaatcat gctcaaaatt
     9241 tttctttgga atcggcaagt gatattcttc gatgattttc ttgtaataaa acaactcaac
     9301 gtcttttttc agatagtcaa aaaatgtttc gccagtcaaa aatcccgact gatgccattg
     9361 ctccaggttt tcttttgtta gtaaccgtgg ccatgccatt gctccgcctt gcttttgatt
     9421 tcttcctcga gggtaataag ggagccccct tcttgatcca ctgtatacaa tcggttcctt
     9481 gcctgatgga tgatagacca gttttccctt ttcgttctcg aagcgccctc ctcttgatac
     9541 tgtaaacaat ccgacataat caaaaaaact taagcctaga cctcttaaaa agacattttc
     9601 tttaggcggt atcttcgaaa gatctacatc tgcaggatta gaaggaagct gatacagtaa
     9661 aacatttttc tttgcgtagt ctacccacac tctttcttct tctgtctcat cattttccca
     9721 gtgaccgctt gctaaaacaa gctgatcgat agaatagctt tgctgatctg tctgtaaaag
     9781 ataaccatct tttcttttgc ttactttttt tactaaagaa taaacaaact gaatagaagc
     9841 tggaaattct tcttgcaact ttgagaaaaa ccagcgttga tagagtccgt ataaacatcg
     9901 agtagattgc tgatcttttt ccagacgatt gatttcttct ataattagtt gcttattttt
     9961 ggttaccgtc tgttcgatat aatctgtagc aggtccttta ctccattgaa acaagttggg
    10021 tccctgtgtt accaatcctc cactggacaa tgtttcatct gtaaaaagcg tcacttgttg
    10081 agaaacagaa ttcattaata actccggtgg ctgattcaac cgccaaactc gacctccagg
    10141 accattggga tcgaaaagaa aaagttcaag agaatgtctg tcctgattat tatggagaat
    10201 cctttctgct acacttaatc ctctaggtcc ggcaccaatg atccctattt tcatttgcat
    10261 gcctccattt cgataaattt tttcaataaa acagcatggt tctgatgttc attttttgca
    10321 ccataaacaa aagtaatgac tggatgttct ttcataagtt gactgagttt aaaaatagct
    10381 tgcttctttt cttcttcgaa aatcaattct tgctggtatt tctttgcaaa tgaagcaaat
    10441 cgttctggta catggtcaaa agttttgcgt gcttcattac taggtgcaat ttctttcaac
    10501 cacagatcta actgcgcttt ttcttttgaa attcctcgtg gccagaatct atccacgagt
    10561 attctatagc catcctcttt ttcagcatca aggtaaacac gttttaattt gatcatagtt
    10621 gtttccttct ttctaccttt tcgcttagct tatatatgta gataatagca caaaataaaa
    10681 aaaatgaacg gcacaactat ccattgcgcc gttcatgtga tcttggtcaa aatctaatga
    10741 ttgattcggt ttttaaagct gtcatacaat catctgtttt atactttttt acttttgatt
    10801 tttccagacc atccgtcata tccaccctta agtaaataaa tctcttgata acctgcttta
    10861 cgtaatttat ttgctgtacg gatgctcaaa cttttctttt gatcgtaaat atagacaggc
    10921 tggtcttttc gaatagatcc taatgaatct ttcaaaacag tgtaagggat gtttcttgca
    10981 cctaaaatat gcccagcatc aaacacatct ttttcgcgaa catcgattac ttgtgctttt
    11041 ctcattcctt ctcgaaattc ttcttctgtc aaagtcgttg ctgaacgttt ggccattact
    11101 cgtagataga cttcccaaag gatgattgca agcacaatca atagcaaaat gatattaatt
    11161 acccataaaa ccatgtaatg cctccgtttt tttaagcaaa tttcaaggct aatgaggcag
    11221 caccaatgac accagcttca ttaccaagct ctgctaattt gatttttgtt gattctctta
    11281 cttgcgggaa agtaaattct ttaaaatatt tctctacacg gctacgtaaa aattctcctg
    11341 ctgccgatac tcctccacct aacacgatac ttgacggatt taatgtattc cccaagtttc
    11401 cgcaagctaa acctaagtag aagcatacgc gatcaacaac catcaatgca aacggatcgt
    11461 cttcttgagc catttcaaag atatctttac ttgttacttc ttctccgttg tctagcattg
    11521 ctttcagttt tgaatcccct gcgtattctt ctgccaagaa acgacctagg cgtaccacac
    11581 ctgtagcgct agatacagtt tctaaacatc cgcgtttacc gcaagtacat tcgaaccctt
    11641 caggatccac agtgatatgt ccgatttctc ctccagctcc gctcacgccg tgaatcaaat
    11701 ttccgccagc aacgatacct ccgccaacac ctgttcctag agtaacgaat acaacgtctg
    11761 gatcgttttc tccagcacct ttccatcgtt cacctaaagc agcaacattc gcgtcattat
    11821 cgatagcgaa tttgattcct gttcctgcct cgatcagttt tttcacaaat tgaacttctg
    11881 tccagtttaa gttatacgca ccaataaccg ttccagcttc gctgtccacg cttcctgggg
    11941 tacccatgcc gattccgata aagtcttcgg ctttcatatt atataaattc aaacgatgat
    12001 tgattgattc aatgatttca ggaacgatat gcttaccatc atccaaaata ttggtatcga
    12061 tgctccattt ttgctgaatc tctccttctg gtgttaaaat tgcaaatttt gctgttgttc
    12121 cacctaaatc aatcccaata atcttcttgt ccattacaaa tttcctttct ttcggctttc
    12181 ttcaattcgg tgctctcttt ttaatatgcg ccacgctgtc atgtatgtat catgatcaat
    12241 caaacggcta tcatacagat tacgcaattc gatagtcatt aattcaatgt catagatgcg
    12301 tgcacccaca tagataaaga tgccaaattt tttgaacagc tgctgtacat catataagct
    12361 ctccatcttt aagcacttcc ttcatattat acaagtaaac catatttttt taaacccagt
    12421 aaaatacata ttacaaaaag aaatacaaaa actagtgtag aaagaatcct ttggtgtatc
    12481 gaatagcttg tctgttgttt cggtagaccc aaaacattcc ctaacactaa tccgccaatt
    12541 agcccaccga catgtcccca aacatctaca gaggtatcca gaagaccaaa aacaaatgtc
    12601 aacacaataa ataaaagaaa atgtctgact agctgctgga tcgctgggtt atgtttaaaa
    12661 tgaaacccta agataaacaa cgcaccgaac aaaccgaata tcgaagtact tgcaccacca
    12721 gacagaacat tcgcttcgtt aaaagcaaag cttgctgtat ttcccattat cccgctgaac
    12781 atataaatca agaaaaaacg ccaatggcca tagattgcct cgatttgctg ccccataaaa
    12841 tacagtatca gtgagttcat tacgaaatgc atcagtccaa aatggatgaa gattggtgtc
    12901 actaaacgcc accattcatt gaagtgaaca agataaggac catacattcc ggcataataa
    12961 gggatctcaa gtcgcggaaa taattccatc atgaggaaga ccgctgtctg tatccctaaa
    13021 aaagcatagg tccataacgg tcgtcttaac catgcacgca attttatttg ctgttgataa
    13081 ttcatgaaaa cctcccattt cattttagtc aataaacaat tgttggatct tctgatcaaa
    13141 gctctccggt tgccattgtt cattcaattg ttcacgaaaa acaaggctac atgttttttt
    13201 atgaaaatta tttaaatacc ggtcgtaata accaccgcca aaaccgattc gataaccggc
    13261 tctattgtag ataatacctg gtacaatgag gagatcgatc atctccggca aatagagctt
    13321 ctctgattgt ggctcaagaa caccaaaact cgaacgtaca agaacggact gttcatccat
    13381 ttcataaaac gccatctgac gtttcggcaa agttttaggt acaactattt tcttttgttc
    13441 tttttctgct cgttggataa ccggctgtgt atccagttca aattcattcg ataaagtcgt
    13501 ccctatcact tttgctgact tccagtactc gctggcgaat agctgtttat agattcgctg
    13561 ttctttttcc tttttcttgc cacttttggc cagatttttc agcttttcga ttcctttccg
    13621 gcgtaatctt actttcaatt ggtccaaaaa ctcacccttc cttctctcat aaaatggtat
    13681 aggaaaaaaa gaaaaatagc aatcgaactt tcaaataaat gcataaatag aataaaaaga
    13741 agacctagaa atgattggcc ttcctttttt aatgaatttc aactgttcag cgcgtgtttt
    13801 tgtctgataa aaagtgcata cagaaaatat ggcgcaccaa aaagggcaat caaaattcct
    13861 gtaggtactt ccccacttgg tagaatcact ctagcaacca tatctgcact aagcagaaaa
    13921 atggaaccga acaaaccgct cagaacaaag gtccagttat tttctttacg tacgactagc
    13981 ttcgcagcat gaggagctag tagtcctaca aaagataaac tacctgtcac cgctacacta
    14041 ccgcttgcca aagcaacagc taacaacaaa aggatttttt gacttttagc cacgttaagg
    14101 cctaatcctt gcgcgcgttc ttgaccaaat gatagcaata caatcctctg tgcataaagt
    14161 cctcctagcg gaattaagat aaccgcccat ggaagcaatg cccaaacata aggccagctc
    14221 gcaccccaga ttgatcccgc taaccatgca ttcacaaatc cataattttc cttagataat
    14281 tggatcgtgc atagcaatgt taatgcagaa aggccggcat taacggcaat accggacaat
    14341 aagattcgat tcatacccaa aaagccattt cgatcatagg ccgcaagata gacacagcaa
    14401 gcagctaaaa caccacctag aaaggctaca actagcaata gaaacggcag cgaatagtgc
    14461 gaaaagaaac ctaaataaag catcactaaa aatcctgctc cggcattaat gcccaatata
    14521 ctagaatctg ccaattcatt tcttgttact ccttgcaaga tataaccaga aacgccaaga
    14581 caagctcctg ctaataaact gatcagtaat cgaggaactc gaaattctct aacaatcagc
    14641 gccaaagaag atgattcttt ccctatgaac gtggcaaaaa gctgatccag gtaaataaaa
    14701 ggcgtaccaa tcattaaact acctatagac aggatgacaa gtacaaaaag caagaaatag
    14761 aacgtccaat atattttttt caccgttgtt ccctccttac ttgtatcagt aaaaacggaa
    14821 cgccaatcaa tgcaatgact aatccaaaag gtgtttcaaa agggggattg atcatcctcg
    14881 ctgtaaaatc cgccaacata accaatccac cgccaactaa aaaagagaca ggtagaacat
    14941 aacgatagtc cgttccaacc attccgcgaa caactggcgg aaccaacagc ccaataaatg
    15001 aaacaggtcc gactaacgaa acggcaatgg ctgctaacac tgcaactaga cagaaaacaa
    15061 tcaaccgcgt tcgtttagga tgacttccaa gagctaatgc atgtgtttca ctcaaatgaa
    15121 tcaaagatat tgcttttcct aatgaaaaag aaaccaaaac agcaagtata tataaaggaa
    15181 acaatactgc taattgtttc catgtgatat tggcagctcc gcctacaaac caaaaagcaa
    15241 gatcctgctg cagattaaag aattgggtaa gtgcttggct gatagcagta aaaaaagaac
    15301 taatagctac gccagctaaa atcagctgta aaggattcat gcccattttc gaaaactgca
    15361 aaaaaccaaa tacgagcaaa gttacagcaa aagaacctgc caatgacatt agaaacacag
    15421 aaaaatcaga agctttaggc caaaaagcaa aactaagggc cattgccaac gaagcacccg
    15481 cgttgattcc tagtaagcct gaatctgcta aaggatttct tgtcacccct tgcatcaatg
    15541 caccgctcaa tgcaaaacaa ctccctacaa gaaatgctcc cgccacacga ggcaaacgaa
    15601 tatttcgtat gatttggtga ctttgttttg atctatcaaa atcaaacaac gcctgccaaa
    15661 aatctgcttt ttccacagtc gctgcgccat ttgaaagagc aacaaaaaaa ataatcaaaa
    15721 gaacaaaaca gaccaaaagc aggagtctat ttttcttcat agtcctcata attgatcctc
    15781 ctgctctttt ggcaacaaat cataggttag aaaaactggt ttatttgttc ttggaagcgt
    15841 gacaatcgtt gcatcaatct caaatatttt tttcatacac tcttttgtga tcacttcttc
    15901 aggtgtacct tctgtgatca acttaccgtt tttcattgct aataaataat ctgaaaaacg
    15961 tgaagcatga ttaatgtcat gaatcgacat caagatcgtt gtttgtttca tttgattgat
    16021 ttcagtcaat aactgtagga tctctaattg gtgtgccgga tctaaaaatg ttgtcggttc
    16081 atccaagatc aagatatctg tttcctgagc aagcgccata gcgatccaga cacgctgggt
    16141 ttgtccacca gacagcgttg cgatatcccg catacgaaat ccagctagac ctgtagcatc
    16201 caacgcccac tcaattgctt catgatcttg cgtgtccatg ccactgaaca gtttttgata
    16261 agggtatcgt ccgtaagata ctacttcttc tactgtcata cctgtagggt gttcactagt
    16321 ttgtgctaat aatgcaattt tttttgcaag ttcttttgaa ctgtatgtag agatatcaga
    16381 gttattgaca atgatctttc ctgatttcac aggtagtata cgggtaaaac ttttcaataa
    16441 agtagatttt ccacttccat ttggcccaat caaactagtg attttccctt caggaatcgt
    16501 aacagacatg ttttcaatga tttttttgtt ttcgtagccg gctgcaatgt cagctgctct
    16561 taatctcgtc atttcaccca tcctttcgct gaaaatcatt ctcaattatg acgctgtttc
    16621 aacatttcgt caagcgctac tttcatatct atagccaaat gaagcggttg acagcgttcg
    16681 aaatatcagg aataataacc aatgagaaag attctcaatt atgttaggag gaaagaaatg
    16741 aaattaaaaa caaaagtatt cacattatct agcatattgc tgctggctgg agtactgagc
    16801 gcttgctccc cctcttcaga taaagaaaca gctacaactg ccaattcgac gacttcatcc
    16861 aatagtactc atacagtcac agatacactt ggacataaag tagagattcc aaacgcgccc
    16921 aaacggatca tcggcagtta cttagaagac tatctgattg cactagggga aaaacctatc
    16981 gcccagtgga ctgttggcag tggttccatt caagattatc tgcaagatga tttaaaggat
    17041 gtcccaacga tttcttatga ccttccttat gagaaagtgt taagctttga gcctgatttg
    17101 ctgttgattt cttcttccgc tactgtcgaa ggcgggaaat acgaacaata tagcaaaatc
    17161 gcgccaactt atgttgtaaa aaacggaaca gatgtcacat gggaagaaca actaaaagat
    17221 gtcggcaaag ctttggcaaa agaaaaagag gcagaaaaaa tcatttccga ttatcaaaaa
    17281 tccatcaaga aaactcgaga agaactagca gataaaatcg agaacaagac agctgcagtg
    17341 ttgtgggtga ccaacaactc agcctttatg gtcagcaaaa atcggtcaag tggaagaatc
    17401 gtatacgacg atctggaatt tggtgttcct gatctagtgg aagaggtctc caaaaaagcc
    17461 acttctgact ggtctgctgt ttcctctgaa aagctagctg aattagatgc tgactacatc
    17521 atccttgtga acagtgacaa agatgctgcc atgttcaatg aaccaagctg gaagaatcta
    17581 aaagcagtga cagacaatca tgtactgcaa ttcggacctg aatctagttg gctatataac
    17641 ggacctattg ccagtcagga aatggtcaat gatatcaaag aaaaactaaa ttgattttaa
    17701 ctaaaaaaag catcttcacg attagtcgtg agatgctttt tttcttatta acaaactaga
    17761 cgcatagatt ttattcttct tttgaagcaa ccctattttt tactctttcc catttttgaa
    17821 aacgatgggc atatagattt tcttgatccg ttatcccctc atactcctct ataagggcga
    17881 agtctgaaaa atcaatattc ccaatatacg tatcgccttc aaacgtgcta tcgatcaatg
    17941 ttcgccagat ccgaccagtg taaggaagaa gttcttgaaa aatccgacta ccacccgaaa
    18001 catagatatc ttctgagatc gtttgactat acgacaaaag ttcatcgatc gaatgcatta
    18061 cttccgcatt ttctttcgtg cgaaaatttg gttgcgttgt caacacgatg atatggcgat
    18121 aaggaagtga aagatccccc attccttcat aggtctttct tcccatcact aaaattcgat
    18181 ccattgtatg ttcgcgaaag aaacgcatat catttggcaa tcgccatggt aacaaaccat
    18241 ttttgccgat cagaccattt ttatcttgtg cccacatgga aataaacata ttatccgctc
    18301 cttatgattt tagatgtttt gagataaaaa aagtctcaat tctttgacca gcaaggaatt
    18361 gagaacttta aaaacattat tttgtttcac ggtgcaaagt aactttgcgt tcgcgtgggc
    18421 aatatttttg tttttccaaa cgatcaggat tgttacgttt gtttttgctt gttagatagt
    18481 tacgttcttt acaagaagta cactctaaag tgatgtttac gcgcatgttt ttccctccca
    18541 tttttacaag aatcctctaa gatgactctc agacttgatt atcttaccat gattttttct
    18601 agattgctag tgtttttcac gattttgtta agtaaaacta cttgacgata aatacaacgg
    18661 ttttacttct caaacaaaat ctttttcttt ttgtataaat acccttacac ttgtttatat
    18721 agattatgtt ttttaataaa gggggatact aatatgccat ggaacatgaa ggactttcct
    18781 gcatcaatga aaaacttaga taaattgaca agaaaaaaag caatcgacat cgcaaatgca
    18841 ttattagatg aagggtaccc tgataaccgt gcgatcccaa ttgccattga tcaagcaaaa
    18901 aaatggtatg aagatgctag tgaatcagaa aaacgaactt tcgaaaaaga aaaaaatcca
    18961 agcaagacgg atgaacacga tacgaatcct cgtgctggaa aactgcttga ttcagatgtg
    19021 atcgtagaat atgaggaaga acaatggatc gtgaaatcta aaggggcaaa aaaggcaagc
    19081 aaccattttg atacaaaaaa agaagcaatc gaaaaaggaa agcaagtagc acaaaacaaa
    19141 gaatcaactt tgttcattta taaaaaagat gggacaaaag aaaaagaaat cgattattga
    19201 acatatgtaa aggggttgtg acgaaaatcg tgtcacaacc cccttatgtt cgaaacaatg
    19261 gtgttcctct gccaactccg aaccataagt cataatatct ataaaacggg aagagctgtt
    19321 ttttgaaatt gtttcttatc actcagtgtt aaacggtagg agaaaacctc ttttttattg
    19381 atatttgatt ttcatcaaac agtcacatag tcgattgctt ctgcttgcgg aatcgtttca
    19441 tctacaaagt agactttata ccatgtcaag aaactgaaga ttgtccagat ttttctttgc
    19501 tcttctttac gtccttcgtg atggtcatct agcaacttca tgattttttc ttgatcaaaa
    19561 aattcttttg caaaatctgc ttcgaataat gctttgactt gttcgtagcc gtgttcttct
    19621 ttcaaccaag ctttgattgg cacagggaat cctaatttca cacgattcga ccattcttcc
    19681 ggaagatgtt tgttcgcagc ttgtctgaaa atatcttttg tgttgttttg attcaataaa
    19741 aacttacttg gtatttgttg agccaatttc atgacttcga tatccaataa aggtactcgg
    19801 acttccaaag aacttgccat agaaagttta tctgccttta acaagatatc ttttggcatc
    19861 cattgatgaa gatcgacata ttgcatctta ttgacttcat ctttgattcc ttctgttttc
    19921 tcgtaatgaa cagtcatgat ttcatccaca gatggtgctg tttgatattt tggtgttaac
    19981 acttctgatg cttctgattc ttcgaacact cgagcatgac cgataaaata ttcttttgca
    20041 ggagcaagtg attcatataa atggatccgt ccatggaagt ttttcatttt tccgatttta
    20101 cgtcctaaat tatagcgtgt tccttttggt aattttttca atccttgagc aatcacacgg
    20161 atgaattttg aattcgtatg gaatccatac gtttggtatc ccgcaaacag ttcatccgcc
    20221 ccttctccag attggactac ccgcacactt tgtgaagcaa gatttgctaa aaagtacaaa
    20281 ggtacgcaag aaggatttga atcaggttca tctaagtgat actggatcaa agggaatgct
    20341 ttgaacgaca tgtctccgtc gatcactgct gctgtattat ctaagtccaa tagttcggtt
    20401 agttttctgg cttcaatcgc ttcattatag gttttatcat caaacccgat tgagaaagaa
    20461 tgatctggtt tcaatacaga ggttacataa ctggaatcaa ccccgctgga taagaaagaa
    20521 ccaacttcta catcactgac cgtatgtgct tcaatagaag cttgaacggt ttcgtcgatt
    20581 ttttcgatcc attcttgtcg ggaatgcgtt tcttttgttt caaaatctgc atcccaatat
    20641 tgctgaatgt ctatttttcc atctttatac gtataataat ggccttcagg caaacgatag
    20701 acatctttga agaatgtttc gccgtttaat ggagaatatt ggaaagtcat ataaggtttc
    20761 aacgcttctt tgttcaattc tttgttaaaa tcaggatgag gcagaaaact tttgatttct
    20821 gaaccaaaca taaatgttcc gttcatttct gcataataat atggtttgat cccaaagtgg
    20881 tctcttgcac cgaataactc attcttttca ttatcccaaa tagcaaaagc aaacattccg
    20941 cggatctttt gcaataattc tgttccccat tcttcgtaac catgcaaaag gacttctgta
    21001 tctgcatgtg tttgaaaaac gtggccagct gcaatcaatt cttctctcaa aggctggtaa
    21061 ttatagattt cgccattaaa aataatgatc tttgtccgat cttcattgta aataggctga
    21121 gtaccgccct ctaagtcaat aatacttaat cttctgaatc ctaaagcaac atgtttatcg
    21181 atatattctc ctgaactatt aggtccacgg tgaacgattc gatccatcat tgtattaata
    21241 attgtttttt tgttgtcctt gtcgttaaca aaacctacaa taccgcacat ttaactttca
    21301 catcctaata taaaattact gtttatttca ttcatatgca tcttatcata gaataaatag
    21361 cggacgaata ttttctctct tacaatttcg tcacgtgaaa acaaaagatg aaagtttctg
    21421 tttttacaga aaataaagtc ccaatatcta atttaccatg ttttcttttt tgaggctacc
    21481 ttattctctt cgtgttaaaa aaatattagt ggatgctatt tccaaaaaaa ccagcaaaaa
    21541 aagacacgac ctaaagatcg tgt
//
LOCUS       DARBFD010000047        21295 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000047 DARBFD010000000
VERSION     DARBFD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.047"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..739)
                     /locus_tag="SCK08_001624"
     CDS             complement(26..739)
                     /locus_tag="SCK08_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1201506.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            914..2338
                     /locus_tag="SCK08_001625"
     CDS             914..2338
                     /locus_tag="SCK08_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1201507.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2637..3344
                     /locus_tag="SCK08_001626"
     CDS             2637..3344
                     /locus_tag="SCK08_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1201508.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3331..3654
                     /locus_tag="SCK08_001627"
     CDS             3331..3654
                     /locus_tag="SCK08_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1201509.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3743..4780)
                     /locus_tag="SCK08_001628"
     CDS             complement(3743..4780)
                     /locus_tag="SCK08_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1201510.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5101..6084)
                     /locus_tag="SCK08_001629"
     CDS             complement(5101..6084)
                     /locus_tag="SCK08_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEG1201511.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6499..7269)
                     /locus_tag="SCK08_001630"
     CDS             complement(6499..7269)
                     /locus_tag="SCK08_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201512.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7262..8197)
                     /locus_tag="SCK08_001631"
     CDS             complement(7262..8197)
                     /locus_tag="SCK08_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201513.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8513..10162)
                     /locus_tag="SCK08_001632"
     CDS             complement(8513..10162)
                     /locus_tag="SCK08_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1201514.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10279..12882)
                     /locus_tag="SCK08_001633"
     CDS             complement(10279..12882)
                     /locus_tag="SCK08_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1201515.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12894..13943)
                     /locus_tag="SCK08_001634"
     CDS             complement(12894..13943)
                     /locus_tag="SCK08_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEG1201516.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13969..14283)
                     /locus_tag="SCK08_001635"
     CDS             complement(13969..14283)
                     /locus_tag="SCK08_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEG1201517.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14299..14745)
                     /locus_tag="SCK08_001636"
     CDS             complement(14299..14745)
                     /locus_tag="SCK08_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1201518.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14746..16668)
                     /locus_tag="SCK08_001637"
     CDS             complement(14746..16668)
                     /locus_tag="SCK08_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEG1201519.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(16880..17776)
                     /locus_tag="SCK08_001638"
     CDS             complement(16880..17776)
                     /locus_tag="SCK08_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1201520.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(17769..18449)
                     /locus_tag="SCK08_001639"
     CDS             complement(17769..18449)
                     /locus_tag="SCK08_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1201521.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(18450..19232)
                     /locus_tag="SCK08_001640"
     CDS             complement(18450..19232)
                     /locus_tag="SCK08_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201522.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(19225..20142)
                     /locus_tag="SCK08_001641"
     CDS             complement(19225..20142)
                     /locus_tag="SCK08_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1201523.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(20306..20629)
                     /locus_tag="SCK08_001642"
     CDS             complement(20306..20629)
                     /locus_tag="SCK08_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201524.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         6310 a         4375 c         3538 g         7072 t
ORIGIN      
        1 ctctgaaaaa ataattttcc cgagcttatt tttcaacaaa aacagcttca aaaacaaagt
       61 cttgatataa atgtctcgat accgaatact cgaaaggttt accagacgat agagcaacaa
      121 ctttgtctat ctcaatcata aattcacctg gatctatttt taatattttt gcgtcaaatt
      181 cattcgcagt atctcctttg atacgtatgt gcgaagtctt tatttccaga cccagttcat
      241 ttgtgatata agcatataca gatttcttta aatgttttgg ttccaagcct ggtatgactg
      301 ctaatggcat gaatgtttgc tcaatcgagt atggttgaca atcaataaaa cggcttcgta
      361 caatattata tacatagtct tttacactga tacttaattt atcagctact tttttcgatg
      421 gaagttcaat tgagaaagta tggatttctg aatctacgtg ttcttcacca accacgctaa
      481 acaaaccttc gtttggacca agaactcgtg agttatcaat tggtgttctg acgacttcag
      541 tcccataccc acttctactt cgcaaaactc cttcggaaac aagtaagtct acgcctttct
      601 ttactgttaa cttgcttgta tgataaataa ctgccaattc atctcctgta ggaagtttct
      661 caccaatttt atactctcca tttacaattt ttttctttat atctaagtaa atttccatat
      721 acttcgcaat ttttttcaat ggtgaccctc caatcaaaaa catatcttca tctatacttt
      781 aacataaata aaccgataaa atccacatca aaattataca cacaataaaa aagatatata
      841 tatttaaaca aaactattta ctttaagttg taagcggtgt tatcattaag tggaaacagg
      901 aggtaaaaaa attatgacaa ctatgaaaaa gctaactttt ccagatgatt tttggtgggg
      961 ttccgcttgg tcagctgagc aagcagaagg aaaaggagaa acaggaaaag cagagactat
     1021 ctgggatcgc tggtttaaag aacaacctta ccggttttat caacgaatcg attcctctat
     1081 tacaaccgat cacatccatc aatataaaga agatatagaa ctaatgaaaa aaactgggca
     1141 taactccttt cgcgtttcta tttcttgggc acgaatgttt ccaaatggcg gtaccggaaa
     1201 agtaaatccc aaagccattt ctttctatcg aaaattattt tccgaaatga ataataacgg
     1261 aatcaaagta tttgccaact tatatcattt tgacatgcct gctactcttc aagataaagg
     1321 aggatgggaa tcacgtgaag tagttgatgc ttatgttcat tacgctgaaa cttgttttaa
     1381 agaatttggt gacttagttt gtcattggtt taccttcaat gaaccacttg ggcctatttt
     1441 aggtacttat cttgaagatt ttcattatcc aaacctcatt gactttaaac gcggagcgca
     1501 agcagcattc ttcactatac ttgcacacgc aaaagcaata caagtattta aagaactagc
     1561 tcttcctagt aaaataggcg ttattctaaa tttgagtcct acttttcctc gtagtcaaaa
     1621 tcctgctgat gtgaaagcag cagaaatcgc tgatttattt tatacgcgga gctttttaga
     1681 tcctatggtt aaagggaatt tttctaaaaa gcttatcctg ttattacaag aatataatca
     1741 gcttcctgaa gattatacga aagaagatct tgtatgtatc tacgaaaata ccgctcaaat
     1801 tcttggacta aactattacg agccacgtcg cgtcaaatct cgttcttcta tggtggatac
     1861 atctagtcca tttttaccag agtggttttt cgaaccacat atcatgcctg gacgacgaat
     1921 gaacgaatac cggggatggg aaatttacga aaaaggaatt tatgatttgt gcatggatat
     1981 caagaaccat tatggaaaca tcgaagcatt tatctcggag aacggaatgg gcgttgcgga
     2041 tgaagaacgt ttcatggatg atacagggca aataattgat gattatcgga ttgagtatat
     2101 caaagatcat cttgcttact tatggaaggc aataaatgaa ggatgtaaca ttaaggggta
     2161 tcatctttgg acatttattg attgctggtc atggatcaac gcctataaaa accgatatgg
     2221 gctggtttca ctcgacctta ctactcaaaa aagaacaatt aaaaaaagtg gcgaatttta
     2281 caagcaatta agtgatacaa atggatttag atacgatgta gacttgttag ttgaataata
     2341 ttctaaaatt ccacttgaag tgaacacatt agaaaacaag aataatgaga aataccaata
     2401 acaaatactt tttcttcata cttcatttat acttgtcagt ataagagact gtgacaaatg
     2461 tagtgatcct cagaagttag gtctggaaat ctaacttgag aagatttttt taaactacgt
     2521 ccagtctttt ttatttgttt aaaattgaag ttgacaagtc ttcattaaaa atttagaata
     2581 ttcacaagtt tgacgacaat tatgaaaatt tatgtcgtgc taaaggagtt ttgttattgg
     2641 atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt tttggttata
     2701 tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cgggtttcat ccgctggtcg
     2761 tcacgttgat gtccctgctg atctatgctg gttctgccca atttatcacg gtcagcatgc
     2821 ttgctagcca tagtccactg ctctccatcg ttctctcgac ctttctagtc aattcccgaa
     2881 tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg cttcaaaatc
     2941 tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat aaattgaact
     3001 atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt tcttatttga
     3061 cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact gatccgaaaa
     3121 agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat cttcaagtca
     3181 tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc actttgattc
     3241 tggtatatgt cggactgatt tttatcccaa gtaacttagt gattttagtt gtaacactga
     3301 ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac attgctgact
     3361 attttaggtt gtactttagc aacttggctt tctaggatcc taccatttgt cttactgaaa
     3421 aaattcgatt tgccgcaacc gttgcttgag tatttaagtt ttgtcccgat cgtcattatg
     3481 tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc gcagatcaat
     3541 ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc aaaaagctta
     3601 ttggtgatcg tcttggtagg aattctttcc ctttcgttga ttcgcttatt ataaataaaa
     3661 aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa gtcaaataag
     3721 aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg acttttccta
     3781 atatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca ttcatcccat
     3841 ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa gcagcaatgt
     3901 gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta gcgaacatat
     3961 actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac ggctctttga
     4021 tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgtttgatt tgatcaaagt
     4081 agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct aattcacgaa
     4141 aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt tttttcagcc
     4201 attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa tgcgctagtt
     4261 gattcaaaat cgaaccgacg cccccagctc cgttgatcac taagattttt ttcccgcggt
     4321 tggcattttc ttctggagtc agaccaaatt tttcaaataa caattcataa gcggtcaatg
     4381 aggtcaacgg caaggctgcg gcttcttcat ctgaaagatt ttttggtgca agagcgacaa
     4441 tacgttcgtc aaccagttgg tattcttgat tgcttcctgc tcttgtcgtt gtacctgcgt
     4501 aaaaaacgcg atcgccgaca gaaaattttt gcacttcatc acctacagca acaactttcc
     4561 cgactccatc aaaccctaag atacgtaaag catttcttat cccgttatct tggcgaagtt
     4621 tcgtatcgac aggattaaca gatactgcgt tgacttttac cagaagatcc cgtgaactgg
     4681 gtacaggtat ctgttctttt tcatcaataa agctttttgg atcatcgatc gacaaccctt
     4741 cataaaaacc gactaccttc attaattcct tttctgacat acgaattcct ccttagcttc
     4801 atcaagttgg agcatccata cagttttcat ttcaatatat atagtatgct atttgttcca
     4861 cttttattca tcataattat tctcttactt aaagtaaact atttgcactt ttttggttaa
     4921 aaaagaggtt gcgacaagac ttttgtcaca acctctttta aacggtgttc aatcaactga
     4981 ccctcggtat tcgctcaaaa aacgacaaaa tatgaggagc tatttttgtt tggctgttct
     5041 tattacttga tgcaggacag ctttttcacg acctctaatt cacggtgttc aagcaagaaa
     5101 tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtgtctaaga tctctcgtac
     5161 ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc ctggaaatcc
     5221 tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga tgatttctga
     5281 ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt tttctgtatg
     5341 ttccctcggt tctttttcaa atgcataagg attcttgaaa atcccccggc caatcatgat
     5401 gccatctacg ccatattttt cggccaattc ttctcctgtc tggcgatctg ggatatctcc
     5461 gttgatcgtg atcagtgttt gcggagcgat ttggtcacgt aaagcaatga ttttagggat
     5521 gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat ggacagaaag
     5581 attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag acatttccgt
     5641 atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagccg cttcaatcaa
     5701 ttgtgccgct acttctgggc gaaggatcaa gccactgcct tttccacgtt ctgctacatt
     5761 agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg ccatagcaat
     5821 gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca tcggctgttc
     5881 atcttctgta aaggtcaatc ttcctcgtac actgtctttt ccatctggat gacagaaact
     5941 gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct ttacgacatg
     6001 gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag gctttggcag
     6061 ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa gcagtgaagt
     6121 cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa gcaaagtgaa
     6181 cagcttaaaa gatgtttctg attgagcaaa aaaagactgt gacaaaaatc ttatcacaat
     6241 tccttttccg gaataaatgg tgttcaaaag cttgcatctg cggtaataag tccaaccaaa
     6301 cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt
     6361 tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt agctcaacaa
     6421 acgacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg tcaaaacgct
     6481 gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca aataccaagt
     6541 aacggcgtac aaaacaacga tgaatagcac aagtacgatc atcgatgttg ctactggcac
     6601 atcgattgtt ccaaggaatc cgtaacggaa accggagatc atataaacga tcggattgac
     6661 tttcgaaaca gcttgccaaa atggcggcaa catagagatg gcatagaaaa cgccacctag
     6721 ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcgtcat aagactgagc
     6781 aaaaatcccg ttgagcagtc ctgctaacga aaacaagatt gccgtcatca ataatgtaat
     6841 gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa tgatcgttac
     6901 gagagagccg acgagaatac tgcgacccac tccgccaatc acaaagcccc aaatgatcac
     6961 atgcgtagga acaggcgcta ccaagatttc ttcgatgttt ttctggaatt tttgagagaa
     7021 gaaggacgaa gaaacgttgg catacgaact agtgatagca gacatcataa tcaaaccagg
     7081 cacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgaccac cgatcatttt
     7141 tccgaaaata acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg tttggaccca
     7201 gatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag taaaataaag
     7261 attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaaa aacagttctt
     7321 ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt gtcagctgat
     7381 cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc tgttcatctt
     7441 caaatgtaca agcgtatcct tcaatcacag gtttggtctc atatggcgct aaatcaaaga
     7501 taaatgtttc aaattgcagt ttggatagaa gctctttcat actagtattc tcgatcagtt
     7561 cacctgattg gatgatcccg atgttgcgac aaagcatttc agcttcttct aaataatgcg
     7621 tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg gagaaactcc cacatctcgc
     7681 gtctcaattc aatatcgact cctgctgttg gttcatccaa gatcagcaaa cgtggttcat
     7741 gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc atacgtgcac
     7801 ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg cgtttcatcg
     7861 cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc tggaccgttt
     7921 caaagggatt gaaattgaac tcttgcggga ctaatccaat ctgttgcttt gctcggacta
     7981 gatccgtatc cagatcataa ccgaagacat tcacttttcc agatgtttta ttgactaaag
     8041 aagtaatgat tccgatcgtt gtagacttcc cagctccatt tggtcctaat agcgcataga
     8101 aatctccttc tttcacggaa agatcgattc ctcgcaatgc ttcgacccca cttccataaa
     8161 cctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct ccttatagac
     8221 atgattcttt attgtatcat ggaatacagc agatgaaaaa acaacttggt tccagcgaaa
     8281 aatcaggttg ctgggctcag gaaaatctcc taatgaaaga catcaatcgg acgaagtcag
     8341 acaacctttt cagaaaaaac aaatcgaaga taaaggatta ctattcatat gtgcaacaaa
     8401 aggagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
     8461 cggaagcaaa agccaagatt tttgatccaa taaatacttt cgatcacttc gcctatccca
     8521 atacatgcca ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg gtttctacta
     8581 aagtctctgg gtaatccaaa actttcagca aggcaatcgc atttcgggtc cttgaaggtc
     8641 cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt tcttcaaagt
     8701 ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa
     8761 atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa gcaatccgtt
     8821 cgatcgtatt tgtccctttt aaaatctcat ctacgaagca gtagcagcgt tctttggaag
     8881 ccacttggtc caacaaacgt ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt
     8941 caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc agggtaaact
     9001 gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct atacttttga
     9061 catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc tgccagttga
     9121 ccggattcac taccgcttct tttaacaatg gatgatagat atctttcgct gtcacaccgc
     9181 cctcagtaaa ctctggtcgg caagtgatgg gcatataagt acggtaattc aatattgcag
     9241 cggctacttc taattctccc agcaattccc acagcttgat ggcttcagtt gaatgctttt
     9301 ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg atgttgacat
     9361 attcaaaaat caactctgct tcactcccac tcttcatacg aaatgaaaaa cctaatttag
     9421 ggatgtctct taatggttta gtttgctgtt tcagctcttc ttgaagtggt gtcggcagtt
     9481 ttgctagctg attggcggct gaaatcgttg aaaccaaata actcatgcta ttcagctctg
     9541 tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaac ccaccgattg
     9601 ccaaatagat tcctagtact tgtcctaaaa gaagcaaacc caaacccacc agcggaagca
     9661 agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca tccgctagat
     9721 attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat tggatttttt
     9781 ctctcagttg aggattttcc tgataaaaag caatcagttt ttctaactgc tgatcttcac
     9841 caaaatcaaa gctgcgtaaa cgttgataca acgcttctga acctacacta gaataggtac
     9901 tgttgattcc ttcgaatact tcgaacatat ccaggtcata ccaagtaaga tcatcgattt
     9961 cgctatccca tgaacggaac ttcttctccg tgagccatgc ttccttcaag ctttcttctt
    10021 tatcaaatct cggttgataa ggaatttttt cccatttgct acgaactgtt tcctttaatt
    10081 tcaaacgtga aaaaatgtct aaagcaacta ataaaataac agctgcaata ataattaaaa
    10141 cgataaattc tctttgaccc atatccctct ccgttccttt atttgttagc tttagtatag
    10201 cactgttgcg ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa aggtgttttt
    10261 ttcttgtata ttacatattt atctctcatt tatcaaaatt gacaaataat catagggagg
    10321 aattgttgtt aattcttgtt tattctccag tgcattgaca actgtcccaa gggaaaggat
    10381 agcctcaggt actatttttt cttgttctgt tggattagct aagcgaagaa caaatccttg
    10441 ttcatgaaaa taagaaggat aaatcgatga tacaattaag tcttcaggta attcaatcaa
    10501 tgaaaattta cgaggaacat caagttcatt ttgtaatggc caaatcttgt tatctaacct
    10561 gtgaataaat acattcagtt tctgcttttg ataagaaata gattgttcta accgtttttc
    10621 tgcctgtttt gccactttga actcattaaa tcttccttct tctacattaa atccaaaagt
    10681 gactttatac tcacctatct cttgagctag cggtgtttcc atcatgacat gcccttcatt
    10741 tgtcgtatct cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc caagttcccc
    10801 tgttgttgaa aataaggtta tgattaattg attttctata cgttcatact cttttaaacc
    10861 atctgcataa aacgtaaaat aatgatcatt ttctgccaca gagacactct tatcgaatat
    10921 ttctaaatta acaggtttct ctaccatagt ttctgaccaa ttcggaggaa tgacatcagg
    10981 ggtattttca ataaatccgt tttgaatttg tgcaattgat ttttcagatt tatctaaaac
    11041 attaattgct aagcgcatac gatgcgagta aacagtatta tgaattttga gtgtgccttc
    11101 aacgatttga ctttctccca gactgaacga tatttcataa gatatttctt ccatttcggg
    11161 tttttcagat aaacgatctt ttaaatcttt aggaagttga gcttttccgt aaacaactaa
    11221 tgtttcttgt aaagaatctt tatatacttt agctgtttct aattttagag atagttctgt
    11281 atctccttct aatggtgaat aatcatacgt atcgccatca tttgctgaat cgatcaaatg
    11341 aacgaaatca agatattgtc tatctccaac tatcaaattc aattgcccat ctttaaaaga
    11401 aagtttatat ttattgttac ttatctcagt tttgtctatc tcgattgctg tttctaattt
    11461 ttcattattt ttctcaaatt caacaacagt gtatcctagt gctggcacat ttacatttat
    11521 ttttatgtca agttcataat aagctggttc atcaaaatat tcgaaacctg tagcaatcat
    11581 ccgataagca tttttccgtt ctggaaaata aacttctttt tcaatgatat tatagggact
    11641 acctttgaag tgtattttct ttgaatcagc tactacatgc acgattttcg atccactata
    11701 attctcagtc gttgtgttga aaacaaggac ttcattcgat tttagcttta atgcatcagc
    11761 catccgtttt acaataaggt tttcgattcc gtcagctatc tcattagctt ctttgattcg
    11821 atggaaaata tcttccgcaa cattatctga aacacagcca ccaatactat catgagcctg
    11881 gttttgcaaa atctttttcc ataaccgacg aagtaggcct ttactgatat ggataccatt
    11941 tctttctgca atcagcatga gcggctcaac tcgttttaga attttttgtt ccaatttaaa
    12001 attttctatt ttcatttttg ttcttactga gccaatagaa cgatgaacac gagagtaaac
    12061 cggctgacga agttcacctt tatattcagg taattctttt ttgtttctga taacttctac
    12121 ataagaagga taatctgaaa taatattgtg gaatttactg tatttattca acttctctac
    12181 gacttgatca aaatttaaaa tcattgactt ttgatctaca cccgaaggta ctaaaatatc
    12241 tttgtttttt ccttgcatat gaagcatttc aactgcttca tccaactttt cttttgcaaa
    12301 ttcaggaata tctttaattg cgtctaaggt catcattcca gtcatataac caaatggaaa
    12361 attcatggca taaacaaatt tatctcccaa tcctttccag ataaaataag gagatggaac
    12421 catcgtttca aagtttaatc ctcgccaaca catgaaatta tcgatttcta cttgatgcaa
    12481 aagtgtaggc aaattcgcat tgaacccaaa cgtgtcaggc aaatacccta ccatcattgg
    12541 atctccatat ttaaggatcg tatcactgat tccaatcatt aagtttctta aaattgattc
    12601 agcatccact aataaggcat ctgtttgtgc ataccacggt cccacgaata gttgtcctgt
    12661 tttcatcagt cgttggataa ccttaagctt ttgcggattg atctccaaat actcatcgac
    12721 gattgaagtt tgtccatcta aacaaaagtt tactttaggg ttcctttcca attcatctag
    12781 aacttcagta aagacctgat cagacaatac gatggcatct tcatttgtaa aataccattc
    12841 tctgtcccag tgcgtgtgtt gaattatatg tgcatttacc atatcagatt ctcctacttt
    12901 ccttcttcta ctgaatttac ttgttgttct tttttatact tgatggattt tagtgcattg
    12961 accattaacg ctgtaaccac tcccccaata acaattgata ctaaatataa aggtatattc
    13021 gtaacaaaag gaattgccat aaaggttgaa agcattgttt cttgagtaac accaaaaaat
    13081 ccatttagac cacatgctac tgcagtgccc accacgatgc ttggtattac tcgtaaagga
    13141 tctttcacgg caaacgggat cgtcccttca gtaatcccca caaggcccat aatcatagct
    13201 gtctttgcat tatctcgttc cgattcatca taaagttttt tgtttaaaat tgttgctaaa
    13261 aacattccaa atggcggaat agcaattcct aataacatgg ctgtactagg tccaaagctt
    13321 ccgcttgaaa aagaagcaac cccaaaagca tatgcgattt tattgatagg tcctcccata
    13381 tcaaacgcca tcattcctcc taaaatagcc gacatcaaag cagcatttgc tcctgacatg
    13441 ccttctagcc aattagtcag tcctgttgtc attgcttgaa taggcgtagc tagtacatac
    13501 cataatatga ttccaacaac taatgtagta acaaaaggaa tcagcataat tgattttaga
    13561 ctaataactt gcatcggtac ttttactttt ttcaatgcat tagctaaata gccacaagct
    13621 aatccagcaa ccattcctcc aataaatccc ataccaagat tttgacacat catcccagtg
    13681 ataaaagctg gtgctagacc gggacggtca gcaattgaat atgcgatata ggctgaaatc
    13741 atcggtacga taaactgtaa tcccgtctga ccaatcattc gcaggactcc ccagaatgta
    13801 tccgtctggt cccaaacacc tgtgccacca aaaatgactg caatagaagt tagtaaccct
    13861 gctgctacga caagcgggat catataggaa accccacttt ggaaatgatt tagaatttct
    13921 cttccttgtt tcttgaattc cattagtaac tcccccacga tatatttttt atttgttaat
    13981 taatgcaatt gcttcatcaa tcgtctcttc accatctttt actacctctg aggtccctac
    14041 tgttaaaatc ggtttaccgt caaagcgacc tcgttctgaa acatttgtat ctactgcaaa
    14101 tatgacaaca tctgcctcgg aaacatccga aattgaaagt ttgttttcta tccccatagc
    14161 cccctgtgtt tctactttaa tatctaaccc tttcttcttc gcaaactttt ctaaattagc
    14221 ttttgccata taagtatggg caatccctgc gatacatgct gttactgcta ctatttttgt
    14281 catatgaaat tcctcctttt aaaatgatag agcttttacc aattcttttt tatttctcga
    14341 atcttttatt cttttaacaa actcgtcatc catcaatctt ccagataatt ctgctaaaat
    14401 acgtaaatga ttagtacctt tatcagtgtc ttttattgct aacaaaaaga cgagatcaac
    14461 tggttgatca tctaatgcgc cccattcaac agaccgcttt aacttggcta cagccactgt
    14521 tgattcaatt actgctaggc ttttgccatg aggaatggcg atatttttcc caattcctgt
    14581 ggtcatatgg ttttctctgt caaatacgtc tttttgaaat tgtttgtggt cagacacaaa
    14641 cccattagtc tctaacaatt tcgataattt ttcgactaca ctttttttat cagatgcatt
    14701 taaatctatc actatattat ttatattgat tttttcactg atcatttata agccttcttt
    14761 caatatataa actaattttt caaacgacga actcgttttt aaatttgcta ataactcatg
    14821 attcgaagcg atttcattga atgtttcata aattttattc aattcaggtt ttactttttc
    14881 attaagtgca aaaaagaaaa cagaatgaac aagtgtccca tcccaatcaa ttccttctgg
    14941 attaacgtag acacagatta ctggcttttt tataaaatct acttcgatat gcggcatagc
    15001 aaaatcattc atcgctgttg aagatagttt ttctcttttg attgctccct ctagtatccc
    15061 ttctttcgcg taattttctt ttaccaattt tttggtaatt atatttaaaa cgttcacttg
    15121 attatccttt atttggtggg taaaaaagca aaatcgttta tctaataaat ttagaaaagc
    15181 atcttctgtt tgttcattta actgtagata ctgttttact tttctgacat ctgcccctaa
    15241 catcaaagga ctaacaacaa taacaggaaa atttgtattt tcaatcggaa tcgtgcttat
    15301 aacaagttca tcagtgactt ttctatcttg tagttcattg atgcttagta catcttctat
    15361 ctcaaccata tctgcaaaac cgttatttat tctttgctct aataattttg atgttccata
    15421 tccgctacta caaactaata tcactttcgt tttgtcaggt ttgtaacgat ccaaaagtga
    15481 ttgaatatgg agtgttacgt aagcgatttc atcttcatta aaatgtatac agaatctctc
    15541 ttcgatttcc tctaataaat ctacacttat cataaatgac cttttaaaag aatatttgat
    15601 tttgtccgta taagggttat agatatttac tcccaatttg agtcttttaa ctgctgcatt
    15661 gagatgtaat actagattat tgatcaattc ttcatcagga taaaattcat ttaatttaat
    15721 tcttactaac tcttgtagct caacacccat atctaaatag gaaagcttcg tttgatctaa
    15781 gtcgtgattc atagcatttt tttcaatcga agtaatgtga atgtcaacat aagcttgttc
    15841 atctattggc acaataattg aaaatgcttt ttcaatcatt ttgattaaaa taagtgtatt
    15901 ctttgagatt tcttcttcag aattattact acgtgataag aaattgcttg tttcaatcct
    15961 ttctatcgca atagcaaggt ggatagctaa agtttgaaat tcataatctg taaatttcaa
    16021 ttgtgtctta ttcatgtatc gattaagcaa ttcggctact tttttcaata tgtccttatt
    16081 taagaaatta ttcgcttcac caatgaaatt gatattcaag tctaagagat ctgttttact
    16141 gttagatgct gtaaagccac cccaataata gttcacaact tctgacatca accttctttt
    16201 acttttttca tcagctttga tgcgcacgcc atttcgatct gaaactaagg acatcccatg
    16261 cttgtttagt ctttttctaa catctttcat tgtgctttct accgtcgacc tgctcacgta
    16321 taattcgtca gcaagatttt gaatctttag agaatcatta gtattcaata atttagcaaa
    16381 cacataagtt agacggtcct ccggagagtc tgcgatattg attttatgac tggataataa
    16441 atttcgaagt gtttcttttt ttccaataag cgaaacgcct tttttaggtt gaaccaacat
    16501 ttgtacatct ggcatttttt ggattatttc tttcaaaatt aaaatatctt tgctaattgt
    16561 tttcgtagat acatataatt gttctgataa ttctttgtaa gttgtgtgct cattttcaat
    16621 caaaaagtat agtattttca tttgccgttc gctaaattcg atgcccatta tcttcacctc
    16681 ctgaatacag cttaagtgta caattattat ctctattttc atacagaaga aagttcttga
    16741 ttaatgtaga agtttctaat attcttgatt atttcaaatc aataaaaaag cctgcaatat
    16801 ttatatcaca ggcttttttc tctattaaat atatataaga taatacattt ttctactagt
    16861 tctcactaat cttgtataat taaaccctcc gcaacttgac ggagatggag aagatgcccc
    16921 cgtctaatct agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa
    16981 gcccgattcc gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga
    17041 agactttttc aacagaggtt tgcgttcctg gtttgatagg attggccact gttaatcgaa
    17101 tgaatggatc ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat
    17161 tctgtatgat attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat
    17221 ttatcgagtg ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag
    17281 agaaaaatac ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt
    17341 gcttcgtctc tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa
    17401 cagattgttt gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca
    17461 gtaattgtac ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga
    17521 cgatcgatag tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt
    17581 catccaacca tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga
    17641 ctgaaagacg accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc
    17701 tcttgatttc cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca
    17761 acagccattt aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt
    17821 gcctttgcta gcttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca
    17881 aaatatggtt cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga
    17941 tgttctaaaa aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca
    18001 ttcacattta catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc
    18061 gacagatctt ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaaa
    18121 agttcttcga tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa
    18181 actttatctc tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt
    18241 cggatttctg ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc
    18301 aaatcgacag gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga
    18361 agtttgtatc tctgttcaag aacctcctcc agtaaggaat gaacagcttc atcgtcttcg
    18421 ataatcaaaa tagtttccag caggaccacc tacttaatgt cagatttttg gaaaataaac
    18481 attcctagag ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct
    18541 tgcgaataag ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac
    18601 tccgttagaa attccaagcc tttgattttt gcgaaaacac cgggaatgaa ggccgttccc
    18661 atgatataaa gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa
    18721 gccactggaa cagataatgt ccctaatgtc agcagaagca atacaggaaa tacttttaga
    18781 acataatttc catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa
    18841 gcaagcatgg ctagtcccgt gatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg
    18901 agaagtttcg acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc
    18961 gtaccgtttt gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga
    19021 atccatgcag ccattttttc taccattaaa atccctgttg caccgttgat gactgtatcc
    19081 gttgcttggt ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa
    19141 aaaagcggaa gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact
    19201 tttatttttt cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa
    19261 ataatcttcc agtttttgcc caactaggtg aataccatcc acaggaactt ttgcttctac
    19321 caatgcttga ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct
    19381 agattgtaca aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt
    19441 cgccgaagtg tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa
    19501 ttctttgatc aagtgtcctt catgcaaaat accatagtga gtggcgatct gtgacaattc
    19561 atctagcaag tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat
    19621 gatttctcgc atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat
    19681 caaaaattct ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa
    19741 agagaaattc tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc
    19801 ttcgatttct ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc
    19861 agttaattct ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc
    19921 ctgcaaatcc gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat
    19981 aatagttctc ataaaggtag acttacccgc tccattttcc ccaatcaatc catagatcat
    20041 tccacgtttg atttcgattg atactttatc taatgctttg gcagcaccgt attttttggt
    20101 tataccagtt gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta
    20161 ttgatgagtg aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag
    20221 tgtaaatttt ccaattgaat gtcaaaaaaa acaatcggac aatacgtgtc tttcctatta
    20281 tcaggcagtt tgttatacgc ttgtatcagt ttcctgattc tttcttttcg accaaagtcg
    20341 tttctctgta ttcctcttca ctgaaaattt ttgcttcttt cagatcaagc ttgagtaatt
    20401 ttaccagacc ggttataagg ataactatag caagactccc tcccagaaaa attagactgt
    20461 ctcgcagacg tggttgcgtt gtcttccaat caaagagcgc accaaacagt gctaatccaa
    20521 gtggactggc caccatggta atcgttgtta tcgttgacat aagtcgtcct tgtatatgcg
    20581 ttggaacttc tcgctgaagc cagaccatat aaaaagcaaa agaaaacatt aatccttaaa
    20641 aacggactta tgaaaaataa tacatgccgt ttgggattta caattagcta ccaaataaga
    20701 ggttatgaca aaagactttt cacaatccct tcttccgaat aaacggtgtt ctcctgccaa
    20761 ctcctcggtc acaagtcaca atatttatca aacatgagaa gcaatttttg taaattcctt
    20821 cttatttctt gaagctgacc acttctgtca caacctcaga agcggataaa taatattgtt
    20881 tgatgatact ttatcttttt cccgtttgtt tatagatcga atgatattat agaccaagaa
    20941 tcctacctat taacatagct aaaaacacaa aaaaggcact ttccaaattg tggaaagtgc
    21001 cagaaaagtt aggattaagg taactgcgct cagcattgct tcgcccagta cgattctcgt
    21061 aactcgtgaa ctcgaacgat aatcgcaatt aacgttttga gaactgtgaa gctttacggg
    21121 ctttcttaag acctggtttt ttcttcgtat cattgaataa gtttgaaaaa ccttgattta
    21181 taaaggtttt cccgtaaata tattttatta tatttgatta aatttgaaat gtttgtgttc
    21241 taaaatgtgt tctaatttaa aggcagtata tttcgtattt tagattaaat tttta
//
LOCUS       DARBFD010000048        20476 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000048 DARBFD010000000
VERSION     DARBFD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.048"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            22..1101
                     /locus_tag="SCK08_001643"
     CDS             22..1101
                     /locus_tag="SCK08_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1201525.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1298..1999)
                     /locus_tag="SCK08_001644"
                     /pseudo
     CDS             complement(1298..1999)
                     /locus_tag="SCK08_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2013..2937)
                     /locus_tag="SCK08_001645"
                     /pseudo
     CDS             complement(2013..2937)
                     /locus_tag="SCK08_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3054..4133
                     /locus_tag="SCK08_001646"
     CDS             3054..4133
                     /locus_tag="SCK08_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1201526.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4165..4758
                     /locus_tag="SCK08_001647"
     CDS             4165..4758
                     /locus_tag="SCK08_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1201527.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(4868..5095)
                     /locus_tag="SCK08_001648"
     CDS             complement(4868..5095)
                     /locus_tag="SCK08_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1201528.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5198..7786)
                     /locus_tag="SCK08_001649"
     CDS             complement(5198..7786)
                     /locus_tag="SCK08_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEG1201529.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(7806..8465)
                     /locus_tag="SCK08_001650"
     CDS             complement(7806..8465)
                     /locus_tag="SCK08_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1201530.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8514..9017)
                     /gene="trmL"
                     /locus_tag="SCK08_001651"
     CDS             complement(8514..9017)
                     /gene="trmL"
                     /locus_tag="SCK08_001651"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEG1201531.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9238..10104)
                     /locus_tag="SCK08_001652"
     CDS             complement(9238..10104)
                     /locus_tag="SCK08_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1201532.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10213..10854
                     /locus_tag="SCK08_001653"
     CDS             10213..10854
                     /locus_tag="SCK08_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEG1201533.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(10916..12277)
                     /gene="mgtE"
                     /locus_tag="SCK08_001654"
     CDS             complement(10916..12277)
                     /gene="mgtE"
                     /locus_tag="SCK08_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEG1201534.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12295..13194)
                     /locus_tag="SCK08_001655"
     CDS             complement(12295..13194)
                     /locus_tag="SCK08_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1201535.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13196..13993)
                     /locus_tag="SCK08_001656"
     CDS             complement(13196..13993)
                     /locus_tag="SCK08_001656"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEG1201536.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(13971..14675)
                     /locus_tag="SCK08_001657"
     CDS             complement(13971..14675)
                     /locus_tag="SCK08_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEG1201537.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            14806..15381
                     /locus_tag="SCK08_001658"
     CDS             14806..15381
                     /locus_tag="SCK08_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEG1201538.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15499..16176
                     /locus_tag="SCK08_001659"
     CDS             15499..16176
                     /locus_tag="SCK08_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1201539.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16255..18063)
                     /gene="pepF"
                     /locus_tag="SCK08_001660"
     CDS             complement(16255..18063)
                     /gene="pepF"
                     /locus_tag="SCK08_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEG1201540.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18239..19237)
                     /locus_tag="SCK08_001661"
     CDS             complement(18239..19237)
                     /locus_tag="SCK08_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEG1201541.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19463..20116)
                     /locus_tag="SCK08_001662"
     CDS             complement(19463..20116)
                     /locus_tag="SCK08_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEG1201542.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5923 a         4451 c         3648 g         6454 t
ORIGIN      
        1 aatagacaag gatgaattca catgacgcaa acccatgata acacagtttc acgcaaggga
       61 aaacatcttt cctactcaga acgttgccaa atcgctatcc tgaaaaagga aggctattcc
      121 aaccgtcagg ttgccaaagt gctgggacgt gttccgcaaa ccataaacaa tgagattgat
      181 cgcggaacca tcacccagtt aaaacgccaa acgcaaaatg gaaaagtgta tgacacctac
      241 tattctgttt atgacgctga tgcaggacag gccttctatg aaaagcaacg actgaactgt
      301 ggcagacgcc ccaaatgggc gaacacaaat gccttcattg attgggcaga tgacaaaatg
      361 ttaaatgaaa aatggtcgcc ggacgtcgtg gtcggatttg ccagaaaaca cgagctgttc
      421 gattgtgcca tcattcctgg cacaaccgct ctttatcaat ggattgaccg cggaatcatg
      481 cgaacaaaga acatggactt gcttgaaaaa ctgtcccgta aaatcaaagc ggtccgccca
      541 ggacaccgtc ccaacaagcg tgttctcggg acgtcaattg aagaacgccc taaagaagtt
      601 gaaacacggg aaacgttcgg tcattgggaa atcgacacgg tagtgggaaa caagaccaag
      661 tcggatgccg tgctgctgac gctggctgaa cggcagacac gctttgaagt catcctaaag
      721 ttgaacggga aggacaccga atcggtggac caagcggttc aacagctaag agaacgggct
      781 ggcgctaact tcgatcaatt atttaagacc gttacatccg acaatggctc ggagttttct
      841 gggctacacg atgctctgca agatgtagct gacgtctatt tcagccaccc ctatgcttct
      901 tgggagcgtg ggacgagcga aaaccagcac aaattaattc gccgtttcgt ccccaaggga
      961 agcccgatta gtgaggtgag cgagaaccag attttgcgga tccagcgttg gatgaacgat
     1021 tacccgcgga aaattttggg ttatgcgaca ccacatgata cattcgtcca ggcattcaag
     1081 caagaacggt tggtggcttg acccattatt ccttgataac gatcctcact cgggacatgg
     1141 cttcatgtct ggaaagctgg cctggggcca gaatcgtctg aagactatca tacctcagtt
     1201 cattatcaag gcctcactga tttagaataa cgaggcagtt acggtatcaa acaaatgagt
     1261 tacctgtagt ggctaactta aacttgaaat ttacggacgt aaagcttgct gccaaattag
     1321 cgattgaaac taacgattgc tttctaggaa tcaaggacaa gcgaatacca tttgacgatt
     1381 gatttgcttg ctgatcctgt acattgcgga ccccccacat cgtcccgtag agaatcgcca
     1441 ttccaatcag agtgaagaaa taaaaacttt tgaaactcat attacgacct gcatgaactt
     1501 cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg cgcgagatca acgttctcca
     1561 cttctgccac tttactcgaa attataccaa tacgctgtaa aaattgatcc atgacgtttt
     1621 tcatcaacgt ctgactcatt cctgcttgac ccacatggag cgaaacagat gaaccatcaa
     1681 tgagataaaa accagccaaa tcatctgcat ttagcttttc attcgcttct acttccgata
     1741 gttcctgtac ttcaaataat ggtttatcat tgacttcgac ttgttctaag actgtggtaa
     1801 aattttccga aagcgctgtt tgatccgctt tgattacaat tccagtatta atcgtttcga
     1861 gattgccacc ttgatcaatc tcgccaaagg ccgccataaa catcaatcct aaaagaattg
     1921 gaaaggccaa tgtccaaaac aacatggatt tatttcgcag cagtattttt gtgcgatatt
     1981 tataaaaatg ccaaaacatt atttcccctc ctctagtctc tcaaatcctt gccagtaatc
     2041 tctaaaaaga catcattcaa ggacgcttct ttggttgcta tttttccgta actgatcttt
     2101 tcttcttgta agattgtaag aatcggcaac aatactgtat ttagttcagt agttgttact
     2161 tggagctgac cttttttata agcgattcca tcagaagcta ttgttcgctg acaacacgtt
     2221 ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca tatcttttag
     2281 agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga tctggtcgca
     2341 aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc cttgtcgatt
     2401 cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag ctactgtcgg
     2461 ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt tcaaccgacg
     2521 tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta atcccactaa
     2581 ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat ataaaccgca
     2641 aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt cttgaggaac
     2701 gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc cgaacaattt
     2761 aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag tcgatttccc
     2821 actgccgttt ggtcctaaca gacctaagac ttctccttct tgtacttgca gatcaaaatg
     2881 attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga taatcatcat
     2941 gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag aaaagaaact
     3001 agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct gctatgcata
     3061 aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt ttgctggtaa
     3121 gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta ttcactggtg
     3181 tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta tttcttttat
     3241 gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg atcgcccctg
     3301 aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg acggttggta
     3361 atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc ttttacttac
     3421 gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc aaagatactc
     3481 attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg tcacaagaaa
     3541 cacaattagc cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac atccatgaca
     3601 atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa atgatcaatc
     3661 aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac actgggatgg
     3721 atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt caacactcct
     3781 tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac ttgtttgaag
     3841 gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc ttaaccaaca
     3901 gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg ccggcctttt
     3961 atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct cccggtattg
     4021 gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat attttccaag
     4081 aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa tgataacagt
     4141 agtcattatt gagatgatcc atttgtgacg aaatcgttac aaaccatttt agaaagcacg
     4201 aaagagatcc gtgtgttagg cattggtcat tgtgccaagg atgcgctagt cttatacgaa
     4261 acccatcgtc ccgatgtttt gctgacggac atccgcatgc ctaaacaaac gggcatcgat
     4321 gctgccaaag aaattttggc taaatttccc aaagccatcc ttttgctttt gacgacattc
     4381 aaagatgaag aatacatccg cgaagcattt tccatcggtg tcaaagggta tttaatcaaa
     4441 cagaacttgc aagcgattat tccttctgtc aaatccgctt ataatggaca ggccgtattt
     4501 ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga ataaaccgac gattgatagt
     4561 catgcttatg cttcttttag tgaacgtgag cttgcaatca tcaaagaagt agctgccggg
     4621 aaaaacaata aagaaattgc agatgccttg tacttgagtg acggcactgt tcgcaattac
     4681 atcagccaat tactagaaaa gctagattta cgggatcgca cgcaattggc tatctatttt
     4741 tatcagcatt tgcaataagt cttatctaac acaaaaaaac ctgaatgatg ctccttcgca
     4801 tcattcaggt ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt
     4861 gaaagaatca aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg
     4921 gaaaaatagg cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt
     4981 ctacatcagg actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt
     5041 cattgccaga ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt
     5101 aacgcccccc ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta
     5161 accaacgatc atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa
     5221 aatcgtaggg agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca
     5281 cttctgctgt aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt
     5341 ctccaacaaa tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt
     5401 cattcgtccc actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg
     5461 tatagttccc atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt
     5521 tggtccggag agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca
     5581 tccggcttgc tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa
     5641 tcaacagttc cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct
     5701 gatattgatg gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga
     5761 ttgagcaaca ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat
     5821 ttgcatcaaa ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga
     5881 tacccgtgat ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt
     5941 ggaggacttt gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag
     6001 gtttccctgg attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc
     6061 cccgatacat tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt
     6121 tttccacaat tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac
     6181 gatctttttg attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa
     6241 gaggaataat actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa
     6301 tttgttcgat ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat
     6361 ctttgtctcc tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag
     6421 ccagccatgt atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt
     6481 tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac
     6541 tggcaggcag acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa
     6601 gtagaatagg aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact
     6661 ctgcaaaaag attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg
     6721 ttaaaataaa tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat
     6781 actgtatgcc gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat
     6841 aatggatttc ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag
     6901 aaaagtacaa actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa
     6961 caagttggat aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt
     7021 ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt
     7081 gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg
     7141 ctgcaatacc gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt
     7201 cttcgactag ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct
     7261 ggtagatact gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt
     7321 gatccatccc gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca
     7381 aaccggagat ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc
     7441 ccagcaattc tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg
     7501 aaagatacgc tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt
     7561 taagctgcct tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat
     7621 tttcttgaat atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg
     7681 tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa
     7741 tcgcggcaac tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc
     7801 ttcttctata gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat
     7861 actttcagtt catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta
     7921 ctattgaaca atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga
     7981 atggctgcag ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgttgg
     8041 gcaaccgcat ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg
     8101 tgaaaaggag aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga
     8161 tatttctggt acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt
     8221 agcttatctg tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct
     8281 gtatcacgtg ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc
     8341 tttttcccaa atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg
     8401 ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt
     8461 tccatacgca gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc
     8521 agcttgtcat tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt
     8581 gcttcataca ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca
     8641 ttcataggga tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg
     8701 gttgtttctt ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta
     8761 tatactttat gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa
     8821 aaagcttcta aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc
     8881 cgttttagat gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta
     8941 ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat
     9001 aaaacgatat gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga
     9061 caatttgatt tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt
     9121 acgttagtga agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg
     9181 aaaaaacaac ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta
     9241 aaaactgcta gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat
     9301 cttcttcaaa ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa
     9361 aggtgacatg gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac
     9421 acgctgacgt tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga
     9481 ataactctgt ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt
     9541 ttcttgtggc accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg
     9601 gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa
     9661 attggtcaaa tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac
     9721 tccttctttt gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc
     9781 attcaagaag ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg
     9841 ctgcgcaata gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg
     9901 aacgaacatt ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc
     9961 ttttttggaa aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt
    10021 tacttcttct tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg
    10081 tcctcggtca atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga
    10141 tgctcacttt ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa
    10201 ggagaagatt agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca
    10261 attgctagag gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc
    10321 ccgagtactg gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta
    10381 atgacgatta ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc
    10441 atgcatactg atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt
    10501 cttactcctt caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa
    10561 gggttgcaga tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa
    10621 gcaatcgatt ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct
    10681 gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc
    10741 cgcttaatca ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa
    10801 gaattgaacg taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat
    10861 aaacaaaaaa acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca
    10921 taaaatagcc cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc
    10981 tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta
    11041 gcatcgggat taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc
    11101 ctatgacaaa ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga
    11161 tccccgtaac agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa
    11221 atccatgatc tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg
    11281 ttccagcatt accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt
    11341 cacttaccaa gtcttcatag tgactgatta aagaagcggt cgacataccc aaaaacaaca
    11401 gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt
    11461 ccacattgac cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga
    11521 tgatatcatc gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag
    11581 ctaagaagtc ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa
    11641 ctgaaagcac tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta
    11701 ggtcgcgcag agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat
    11761 agattgtttc agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt
    11821 gattggctac gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt
    11881 aatggagaag ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag
    11941 caatttgctt tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag
    12001 aaagcatatc agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt
    12061 tgtcttcttc aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga
    12121 tcagctgcct gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca
    12181 tctctaaaaa actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact
    12241 gggcaaatct ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc
    12301 ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa
    12361 ctcttctgta aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat
    12421 tcccagatcc attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga
    12481 aaaatgaact cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat
    12541 ttctgttagt cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac
    12601 cattcgcttc aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc
    12661 cgataaatct gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc
    12721 acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaaacc
    12781 agatgtatcc cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt
    12841 tttataataa gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat
    12901 agacgaaacg cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc
    12961 gatcggtgtt tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt
    13021 taccttatca tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat
    13081 ttgaccgcct tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa
    13141 aaaatatttt acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat
    13201 cctcactaat aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa
    13261 aatggatccg ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat
    13321 tcaattgatc aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt
    13381 catcatgggc aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag
    13441 caatctctgc caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt
    13501 tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt
    13561 caaacagctc gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg
    13621 ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa
    13681 catccagcaa gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt
    13741 cttccagctc gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca
    13801 agaaacgcac atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt
    13861 ctccgccgac tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt
    13921 ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga
    13981 tcgctacttt catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag
    14041 tagacatcat ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct
    14101 tcacggatac tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt
    14161 cgttcactca tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc
    14221 gttgcccaga aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca
    14281 ccgctgatca gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct
    14341 tttttatttg tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg
    14401 atctcaacaa cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata
    14461 tcagacattt cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca
    14521 atactatcga ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt
    14581 tcacggaact gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca
    14641 tatggtgcta aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca
    14701 ttcattgttt attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac
    14761 caaaaaaaga aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt
    14821 gaaatcgaat ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat
    14881 caattaacag ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa
    14941 cagctgaaga aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat
    15001 acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa
    15061 gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa
    15121 ctttctgatt tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa
    15181 cgggctgaat tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat
    15241 ctacacgatt ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt
    15301 ataaattttt taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt
    15361 atgcttgaag ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt
    15421 tacttttttc tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct
    15481 attttggggg agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac
    15541 tgtttcgaaa tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca
    15601 aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc
    15661 atgcaacaaa aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg
    15721 acttattctg cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc
    15781 cgttttttga tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa
    15841 cttgctgaat ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt
    15901 cagtctgact tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc
    15961 atcacaaaac acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt
    16021 ttagtagaag taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca
    16081 gataactcat atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat
    16141 tcaagaacta gccatttaca cttgctttct aattaaaaaa atacgtcgac tcccttacaa
    16201 gagagccgac gtattttttt cgtttctatt cgagaaaatc aaaaatagaa agccttataa
    16261 acgatcgatc aattcttcta attcatttag acgttgttca aacatcgaca ttgcatcttc
    16321 aatatatgca gcctgtgtca tatcaactcc tgcttttttc attacttcca caggataatc
    16381 gctgttacct gcttttaggt aagccaagta gttctctagt gcctctggtt cctggtttag
    16441 tattttcttc gccagtgctg aagcagcaga aaagccagtc gagtattgga aaacatagta
    16501 attataataa aaatgcggta tccgtgacca ttcgaattta atttctgggt cttcttcgac
    16561 tgctggacca taatattttg cattcaattt accataacta tcacttaggt attcactagt
    16621 caatggcaca cctttttcat cttccgtatg catgaaatgc tcgaattcag cgaattgtgt
    16681 ctggcggaaa actgttcctt taaacccatc caagtaatgg ttgagtacat aagcccgtac
    16741 acgagggtct ttttctgttt ccaataaata ctccgttagg atattttcat tcgttgtcga
    16801 agcaatttct gctaaaaaga tggagtagtc gccgtacaca taaggctggt tcgaacgagt
    16861 gaaataacta tgaacactat gtcccatttc gtggacaagc gtaaatagct gatccagcgt
    16921 atcatgccaa ttcaataaaa tatatggatt tgtgtcatag cttcccgaag aataagcacc
    16981 gcttcgtttc cctttatttt cgacaacatc gatccaacgt tcagagaatg ctttttctac
    17041 gatggccatg tattcttcac ccattggttt cagtgcttct aaagcttttt cttttgcttc
    17101 ttcgtacgta aaggtaattg gagcttcccc taagaccggt gtataaagat catacatgtg
    17161 cagtttttcc acttctaata aacgtttccg taattccatg tatcgatgta acaaaggcaa
    17221 atgcttgttt accacgtcta ccaaagtatc gtatacactt tcaggaatat gattattgct
    17281 caaagacgct tctctggcag agctgtaatt acggactttc gctttaaaat tatgtccttt
    17341 tatatgtgtg cctaaagtag aagcaaatgt atttctaaat tgttcgtaaa cactgtacaa
    17401 tcccttaaat gctgcttcgc gcacccttcg atccgtgctt tctagcaact gaccatacac
    17461 gccatgagat aattggacta tttcaccatt ttctccttca atcgttggaa aaactagatc
    17521 tgcattattc aaaacagcaa atgtatcact tgaagcatca aagatttcac ctgctccagc
    17581 aagaagagat tcctgctcag cagataagac atgggctcga ttatctacta tttgctggat
    17641 ataatggcga tagacttcca attttggttc ttctttgaaa tattgccaaa tctggtcatc
    17701 tgacaattgc aatatttctg gttcaaacca ggaaacggct tcactaactt tcgaaaacag
    17761 actgcttgct cttgcataaa gcgcttggta atctgtattt ccagtgtctt gatcgttttt
    17821 aaggtgcgca tatacataaa tgacttcagt ttggcgataa accctcaata cgaattcaat
    17881 cgcatttaaa aattgagaag cgccttgatc aagtgttcct ttgtgttttt cagattgttt
    17941 taactcttct gataattcca aatatttctc atcaaactct tggtcgcttg aaaagatctt
    18001 ggtcaagtcc caagttaaat tttcaggcaa ttcttctcgt tttggcaact gctttacttc
    18061 catgaaatcc actccttttc ttcccttagc tctatctatc aaaatagatc atagtctaac
    18121 atccacatgt cacagcatct aacttttcta ctcattcatg tgtctgcttc catcaatttt
    18181 agcataacta aatcttgttt tacatagaaa accgattcct agcctatttt tatacttact
    18241 attttttcaa agaagacaag tgtatgcatt cgttaaaaaa ctgctgatag accatctctt
    18301 catcaatcat aggaaaaagc cattcccttt tatgttcttt tatttggcaa agccatgagt
    18361 tgaaatcagg aactttctgg ttttccaagg cttcttcgta caacattcga taaagaaaaa
    18421 cctgttcttg taaataaaag aaaaaccgac ttttttgata gatccacgat gatagatgca
    18481 aaagatgttt atgacgcaag tagcattgtt cttgtatgga tactgtattc ttttgcttgt
    18541 gaataagttg gcgtgtcagc catttcttcc catcttcact cactttttct atttttttca
    18601 tttggttcat tttaatttcg ttactagtga atatttcttt aaggtcccta ctagaaaaag
    18661 tccagcagct actgcaaaag gaaatatgcc ctgatacagt ctctgtaata tgatgataca
    18721 aataaagctt ttgtttcttg agatcagctt tccagcaatg cactcctctt tttctattat
    18781 agtagcagca acttttttct atcaatgaga attgcgattg tccaagaaaa gaattaccaa
    18841 gtatccacca tacttggtaa ctgttattca aataattttg ggttctttca ataaatctct
    18901 ggtgagacag tctagaacat tggatttcta ttgctagatt cgccaacaaa atatccggcc
    18961 tttgccgcaa cctcggcaaa taggcttcca aaaatactgg ttctactgac tgtcccagcc
    19021 aatccatgaa gcattccttt aactgtaagt gctcttttga ttctctttca ctaaagaccg
    19081 aacagttatc tgatttttga tgggcaaaat gagcacattt tttctttccc tttttcaaaa
    19141 tcactctttt tcgacagcca cgacaataat atttctttcc tttttctgca tgtttagcaa
    19201 aacagcactt attatattca ttcattgcaa gcagcatttt tctccctctg aacttattta
    19261 cgtctaagaa tttatttaca cgaaaaaaga gggcgtgaaa aagctattct gcatcaagta
    19321 ataagaacag ctaaacaaaa atagttccca atatttttcg tttggttggg cttgttaccg
    19381 cagatgcaag cttttgaaca catttatacg aaaaaaagac tgcgacacaa tttttgtcac
    19441 agtctcttac tataatgctg atttatacaa aataaaattt cgttcgctcg atagcattac
    19501 gcgccatcag acattgtcca tattcattca atgtttctgg tgtacgatcg ctcttttcag
    19561 caaactccaa aattcgagca atctgattct ctacatccgc ttggttgaga ttttccatcc
    19621 agaagtgaac gactaggtaa tagcgctctt cgaataaata caaatcagtc caagcagact
    19681 ctagttcgac atctgttgca agttggatca cttgttcaaa atcttcaaaa gcaaaagttg
    19741 cttgtctttc ctgttcttct agggcgcttc cagataattc ttcctccgga tcgctgccaa
    19801 taatctgctg acgaagaaag tcactcacat cttctgaatc aacagtagaa tcttctgatt
    19861 gagaaatttc ttcatccgga agatttttac tgataaacag ttctaatccg tctccttttg
    19921 gcaaaacttg gaaagtgaca gcttcgcttc ctcggaactc ctcttcgata tcaacttctt
    19981 ccaaaatact ataaaagaaa ttttcgattt ctttgtgatt gccaagtaaa tccaaaaatg
    20041 aaacgccccg ttcctctaaa tcttcatgac caattagtac acggatcgta ttttcattaa
    20101 tatgttccat ttccattttg ctacacctca ctttgccaca atgtttaata tagattcatt
    20161 gtagcgaaat cagcttaaat gtaaagaaaa aaaatctcgc tgttaagcga atttaataaa
    20221 accagcggat ttttcaacct ttcaagctat cttatttaac tataacattt ttggcagacg
    20281 aatactatat agccgacttt tcagctaatt ttccctctct atttttcgtg caatcatcat
    20341 cttctactac ctttttatac atctaatcaa gaatactttt tgtaaaaaca aaaaaacttg
    20401 gaaaaactat ctttgtaata gtcttttggc tctatacttt ttgatgttgg tgaacttcac
    20461 caacatcttt tttgta
//
LOCUS       DARBFD010000049        19778 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000049 DARBFD010000000
VERSION     DARBFD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.049"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            99..293
                     /locus_tag="SCK08_001663"
     CDS             99..293
                     /locus_tag="SCK08_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201543.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="SCK08_001664"
     CDS             562..861
                     /locus_tag="SCK08_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201544.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="SCK08_001665"
     CDS             complement(958..1392)
                     /locus_tag="SCK08_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1201545.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="SCK08_001666"
     CDS             complement(1624..2334)
                     /locus_tag="SCK08_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1201546.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="SCK08_001667"
     CDS             complement(2324..3184)
                     /locus_tag="SCK08_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEG1201547.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="SCK08_001668"
     CDS             complement(3189..3827)
                     /locus_tag="SCK08_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEG1201548.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="SCK08_001669"
     CDS             complement(3842..4141)
                     /locus_tag="SCK08_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1201549.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="SCK08_001670"
     CDS             complement(4184..5668)
                     /locus_tag="SCK08_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1201550.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="SCK08_001671"
     CDS             complement(5688..6149)
                     /locus_tag="SCK08_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1201551.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SCK08_001672"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SCK08_001672"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEG1201552.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="SCK08_001673"
     CDS             7452..8222
                     /locus_tag="SCK08_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1201553.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="SCK08_001674"
     CDS             complement(8495..9322)
                     /locus_tag="SCK08_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1201554.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="SCK08_001675"
     CDS             9890..11125
                     /locus_tag="SCK08_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1201555.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="SCK08_001676"
     CDS             complement(12124..12384)
                     /locus_tag="SCK08_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1201556.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="SCK08_001677"
     CDS             complement(12411..13712)
                     /locus_tag="SCK08_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1201557.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="SCK08_001678"
     CDS             complement(14004..14462)
                     /locus_tag="SCK08_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1201558.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15806)
                     /locus_tag="SCK08_001679"
     CDS             complement(14475..15806)
                     /locus_tag="SCK08_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEG1201559.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17259)
                     /locus_tag="SCK08_001680"
     CDS             complement(15817..17259)
                     /locus_tag="SCK08_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1201560.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="SCK08_001681"
     CDS             complement(17246..18010)
                     /locus_tag="SCK08_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEG1201561.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="SCK08_001682"
     CDS             complement(18114..18989)
                     /locus_tag="SCK08_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1201562.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="SCK08_001683"
     tRNA            complement(19244..19328)
                     /locus_tag="SCK08_001683"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
     gene            complement(19346..19417)
                     /locus_tag="SCK08_001684"
     tRNA            complement(19346..19417)
                     /locus_tag="SCK08_001684"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19383..19385),aa:Gln,seq:ttg)
     gene            complement(19427..19499)
                     /locus_tag="SCK08_001685"
     tRNA            complement(19427..19499)
                     /locus_tag="SCK08_001685"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19464..19466),aa:His,seq:gtg)
     gene            complement(19561..19645)
                     /locus_tag="SCK08_001686"
     tRNA            complement(19561..19645)
                     /locus_tag="SCK08_001686"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Leu,seq:caa)
     gene            complement(19666..19736)
                     /locus_tag="SCK08_001687"
     tRNA            complement(19666..19736)
                     /locus_tag="SCK08_001687"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19702..19704),aa:Cys,seq:gca)
BASE COUNT         6276 a         4090 c         3077 g         6335 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
    19381 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
    19441 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
    19501 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
    19561 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
    19621 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
    19681 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
    19741 tactattatc aatctataaa ataaagaata aattatag
//
LOCUS       DARBFD010000050        19603 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000050 DARBFD010000000
VERSION     DARBFD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.050"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..152
                     /locus_tag="SCK08_001688"
     CDS             <1..152
                     /locus_tag="SCK08_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1201563.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="SCK08_001689"
     CDS             complement(230..763)
                     /locus_tag="SCK08_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEG1201564.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SCK08_001690"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SCK08_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEG1201565.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SCK08_001691"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SCK08_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEG1201566.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="SCK08_001692"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="SCK08_001692"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1201567.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="SCK08_001693"
     CDS             complement(4488..5066)
                     /locus_tag="SCK08_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEG1201568.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="SCK08_001694"
     CDS             5388..5858
                     /locus_tag="SCK08_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEG1201569.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="SCK08_001695"
     CDS             complement(5966..7192)
                     /locus_tag="SCK08_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1201570.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="SCK08_001696"
     CDS             complement(7946..8146)
                     /locus_tag="SCK08_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1201571.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="SCK08_001697"
     CDS             complement(8280..8888)
                     /locus_tag="SCK08_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEG1201572.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="SCK08_001698"
     CDS             complement(8973..9443)
                     /locus_tag="SCK08_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEG1201573.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="SCK08_001699"
     CDS             complement(9491..10405)
                     /locus_tag="SCK08_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1201574.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="SCK08_001700"
     CDS             10551..11354
                     /locus_tag="SCK08_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEG1201575.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="SCK08_001701"
     CDS             11532..12479
                     /locus_tag="SCK08_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEG1201576.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="SCK08_001702"
     CDS             complement(12547..12840)
                     /locus_tag="SCK08_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEG1201577.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="SCK08_001703"
     CDS             complement(12868..13533)
                     /locus_tag="SCK08_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEG1201578.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="SCK08_001704"
     CDS             complement(13673..14305)
                     /locus_tag="SCK08_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1201579.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="SCK08_001705"
     CDS             complement(14312..15379)
                     /locus_tag="SCK08_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1201580.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="SCK08_001706"
     CDS             complement(15376..16107)
                     /locus_tag="SCK08_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEG1201581.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SCK08_001707"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SCK08_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEG1201582.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SCK08_001708"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SCK08_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEG1201583.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="SCK08_001709"
     CDS             18270..19439
                     /locus_tag="SCK08_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1201584.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         5911 a         3941 c         3139 g         6612 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttc
//
LOCUS       DARBFD010000051        19028 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000051 DARBFD010000000
VERSION     DARBFD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.051"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(43..1611)
                     /locus_tag="SCK08_001710"
     CDS             complement(43..1611)
                     /locus_tag="SCK08_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1201585.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1615..1896)
                     /locus_tag="SCK08_001711"
     CDS             complement(1615..1896)
                     /locus_tag="SCK08_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG1201586.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(1893..2816)
                     /locus_tag="SCK08_001712"
     CDS             complement(1893..2816)
                     /locus_tag="SCK08_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEG1201587.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2813..3691)
                     /locus_tag="SCK08_001713"
     CDS             complement(2813..3691)
                     /locus_tag="SCK08_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEG1201588.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3678..5015)
                     /locus_tag="SCK08_001714"
     CDS             complement(3678..5015)
                     /locus_tag="SCK08_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1201589.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5018..6121)
                     /locus_tag="SCK08_001715"
     CDS             complement(5018..6121)
                     /locus_tag="SCK08_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1201590.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6195..7703)
                     /locus_tag="SCK08_001716"
     CDS             complement(6195..7703)
                     /locus_tag="SCK08_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1201591.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7669..8361)
                     /locus_tag="SCK08_001717"
     CDS             complement(7669..8361)
                     /locus_tag="SCK08_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1201592.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8383..9357)
                     /locus_tag="SCK08_001718"
     CDS             complement(8383..9357)
                     /locus_tag="SCK08_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1201593.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9657..10529
                     /locus_tag="SCK08_001719"
     CDS             9657..10529
                     /locus_tag="SCK08_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1201594.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11111..13309)
                     /locus_tag="SCK08_001720"
     CDS             complement(11111..13309)
                     /locus_tag="SCK08_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201595.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13541..14533
                     /locus_tag="SCK08_001721"
     CDS             13541..14533
                     /locus_tag="SCK08_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1201596.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14593..16014
                     /locus_tag="SCK08_001722"
     CDS             14593..16014
                     /locus_tag="SCK08_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEG1201597.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16007..17506
                     /locus_tag="SCK08_001723"
     CDS             16007..17506
                     /locus_tag="SCK08_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEG1201598.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17545..18939
                     /gene="uxaC"
                     /locus_tag="SCK08_001724"
     CDS             17545..18939
                     /gene="uxaC"
                     /locus_tag="SCK08_001724"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG1201599.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5713 a         3877 c         3366 g         6072 t
ORIGIN      
        1 agtgaaaata agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc
       61 atcatacttg aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac
      121 tgctatcccg ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg
      181 acctactttc ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt
      241 ctttacagtg actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat
      301 ttgtgctact ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca
      361 agtaacataa caataaatat aattggattc atttaagtac agtaccagtc cagccatctg
      421 attaaaatta ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg
      481 ccgaatagct aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg
      541 taaaaaacct ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc
      601 attccagttc ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg
      661 ttgtacctct tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt
      721 aagtcttaac caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa
      781 aatagccgca ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg
      841 ggtttgtact aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc
      901 tgtcagcata ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca
      961 aactgtaact gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc
     1021 gttacgaaaa tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt
     1081 atagatggga ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct
     1141 ggatttattc ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc
     1201 cgtttcaaga tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc
     1261 ggaccaagga cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg
     1321 acggcctgca cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat
     1381 ctgtggcgcc catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa
     1441 aatatccgtt gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg
     1501 aagccattca aaagaggaga caataatgta ataccgctct ttcactctta atatattagg
     1561 atctggattg aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc
     1621 tccttttctc attcttactc gatataaact gaatgcccca gctgctaaaa aagcaagtga
     1681 aataaacaac acaacggcaa acagaccagt catgtcctgt aataatccaa atgtattttc
     1741 taaagacaaa gttgaatgat ctacagcaag cgactgataa ctttttttaa actctgacgc
     1801 agacatatta cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata gactgccacc
     1861 gaccattcct agcaatagtt cccattttct tttcatttct gaaccccctg cttttctaaa
     1921 tgtatccatt caggtctttt gatcgatgtt tcataattgt tattacgttc agagaatttg
     1981 acagtctgtt catttttctt gtcatcccaa ttatcccaac cagttgggtg aacatgccca
     2041 cctatcttac acgcttcaaa taagacattt gcatacggac gccacggtct tcctaaatag
     2101 acgtttctag tatcaggttc tgcagtcaag gtacagcttt caaaacgata ccctgttttt
     2161 tgatttttcg gcgtagatgc tgctgtgatg tatccaggat ttttactggc taagctatgg
     2221 atatgacact gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac cgttccttca
     2281 atccagcaat attcataggt ctgacgacaa tactcaggat tggcagaagc cggtgtacaa
     2341 aaaggagtac catctttttg caaatcaggc aacggacccg tgcataaggt atcctgataa
     2401 ccagatagtc gacagttttt taaagatacg cgatctcctg aattgaataa agcaacagcc
     2461 tgtcctacct tttctcctgg acctgctgta ttttcgatgt gaagattttc taatacgaca
     2521 ttcgatcctt ctaagaaaaa agtagcactt cggaaagtcc ctaactggct accgtcaaga
     2581 tactcctgtt ttgcaaaacg attaccggag ataactactg ttcctgctcc cacaagttta
     2641 aaattggata atctgctttc tatatattca tcatatcttc cttcttttac tatcaagacc
     2701 tttggatggc tatccggcag gcctgccaaa taatcgattc cttcttggat agtttcaaaa
     2761 tccgtcgggg tatctatagc attctttcca attatcagtt tgtctttcat cctcataaat
     2821 gctcccttat gaattgtttc caaatatttc tcatttcttg tgtttccata tgaccgcctg
     2881 ttaaagaaaa agaagaaaag ttttctggac tacccattga ttgatacttt ttgtccagcc
     2941 cttttctcag cttcagtacg ccttcgtcca agcacatcgt atcgtttttt ccaactaagc
     3001 taagacgagg gcgcggagcg attttttctt ggattgctaa cgttgtataa gctgtcaata
     3061 gatttggcac atagtaataa aacccatgat gatctaggcg gcgatgtagc aaaagtgttt
     3121 cgatgtccac ctgtgccgct agatcaatac aaactttgac acgagaatcc aatgctgtca
     3181 cccaccagct taataagcct cccatagaaa gaccaatcgt tgcgagccgt tgagtatctg
     3241 tgtcttccct tgtctccaaa taatcgatca agctgatcac atcataaata cgcattcccc
     3301 ataacgtttt tcctgttaat aaaaattgtt tgaacaactc gctttcagaa atacctccgc
     3361 gttcttcaaa tccccaagca tcaatcgacc aaacagcata tcccattcct gtcagtactt
     3421 ctgcaaaaga tggagaagct aagtaaggag caccatggat caattcactt ttcccgactg
     3481 aaaaatctcc gccgtgagaa tgcaggtaaa ggaccgtcgg aaacggtcct tctttttctg
     3541 gataagcata gagggctggt actgtttcca attcatttaa gtacaattga tagtattcaa
     3601 gcataaaacc gttttgtttt tctcggtata atcgttttcc actaggggta tgtctgctag
     3661 atggagcacc taaaaaatca ttcaactgca taagcaaagt ttttacgaac aaccagttca
     3721 tccatatttt ctgattgatt tgattggaat ttctcaatac tgatttcttt agtattttct
     3781 aatagaaatg ctggtccctc gtgattttca atcgacatct ggttaaagga aaccttaccc
     3841 gcaaagccaa tataaaatcc ttgtctgctc atttcctcta ttcctgtcat catagcagga
     3901 acaccgggaa ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt gatttcttgt
     3961 atgaattgtt ccgctaatcc ataaataaaa cctgcagaag catggacatt ccttgccgtg
     4021 atattcgaaa aatggatcct tctgaatgct gatgtccgtt catctatcgg ataagccttt
     4081 ttttcccata catacggttc tttccctcgc ggaccacaga aataataaag attcagtata
     4141 aatggacaca tcacattatc catgactata ttggatactc gaatatcttc aacgatcccg
     4201 cctcgacctc gtctagattt caatcgtatt cctcgatcag tttcttggaa gatacaattt
     4261 gaaatggtaa tattacgaat actaccactc atttcacttc ctaatacgac accgccatgt
     4321 ccatggacca tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct ttcgtacgta
     4381 tcttcagtcc ctgccttgat tgcaatacag tcatctccca cgtcaatatg gcaattacta
     4441 atacgcacat ttttgcatga ttctggatca attccgtctg tatttggtga atccgctggg
     4501 ttcaagatcg tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt gatcgtccaa
     4561 cttggtgact ggatcaattt aatatctttc acagtaattc gatgacagtt atggaaactc
     4621 atcaatttag gacgaggata agcgagatta tctggttcat ttctaaaagt atgccaccat
     4681 ttttttccat ttccatctaa tgtaccgaaa ccagtgacag agatgttctc tacattttga
     4741 gcatagatac aggaagcata aacttttcga tggactcctt cccaacgtga atgaacaaca
     4801 ggataatctt ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa atgaagttcg
     4861 acattgcttt ttaaaaataa agctcctgtt agaaattcac ctgctggaac aactacggtc
     4921 ccgcctccgt cacttgctgc agcatcaatg gcttgttgta ttgcttcagt attcaattca
     4981 tcaattgatg cgccaaatgt caaaatatca tacatattta taatgcctcc acttcaatca
     5041 atgcttgcaa gaatgcacca acgccttttt gatcattaca aataattggt tcactgatgt
     5101 agtaagcata tgaaccatct cgctgatctg ctccgcctaa ccctgctact tgacagtttt
     5161 tattcaagtt cagccagcct tcttttgttt ccagaacaaa ttcatcaagt aatccttgat
     5221 gtgatttctt cacttgtgga agccattctt ctttgttcag cagtcccaaa cgtattcctt
     5281 tagccattgc gtaaacaaac atactactgc cggaagcttc cagataattt cctttccgtt
     5341 ttgtttctgt tgttacttga taccatgtcc cgctgttttc ttcttgatat ggaagtaatg
     5401 cagccagtgt cttttctaga atttctttca gcaaagaaca atctgtttct tcatgaagta
     5461 attcataggt atctgctgca gccatcaaat accagccaat cgatcggctc caaaaatgat
     5521 gggatagacc tgtttttgga tcagcccatg gttggacctt cttttcatcc catgcatgaa
     5581 tcagcaaatt tgtttttgga tcgaatagat gtttataact gatttcaaac tgtctcaaaa
     5641 catcttggta atctttttct tcactaaaat tctggataaa ttcagcataa aaaggagaac
     5701 ccatatataa gccatccaac caaatttgat aaggataaat ttctttatgc caaaaagctc
     5761 cttcggatgt gcgtggatgg tttgccaact gctgggaaag gagttctgct gcttttttat
     5821 atttttcttc gcctgtttct ttgtacaata aaaagaaaag ctttcctgta tttacatggt
     5881 cgatattgta ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc acgaaatagt
     5941 cgatattgtc ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc caaagtaatt
     6001 ggaacccttt aagtacgacg ccatagtcat aagaccattt tcctttatac cacttttctt
     6061 catataagcg aggtgttctt tccatgatac tttttgctaa tttctttgac cagttttcca
     6121 ttatttgctc tcctttaaaa attaaaatag gatttgggac aaataccaag aagtatttgt
     6181 cccaatcgtc tatattactg tgccgctgct ttgtatgctt cctcgtactc ttgtgtgatt
     6241 cgatcgcctc cttgtgcttt ccagtcttct acagcttttt tgaatccatc ttcgtcaagc
     6301 tgtcccatga tgtattgcac cgtagcatca atgacgattt tttcaatttc tgatcctact
     6361 tcattgttcg tcggtgaatc caatggcaca gaaggatcca ttacagcaaa ttcttcattt
     6421 tctttgatca attgattaga gaattctttt tcagggtctg tatctttgaa agagaacgtc
     6481 acttcactag gacgagagct tgataacggt tgcacctctt gttgccaaag atccatatcg
     6541 ataaattcga tagaaccatc atcattcaat tcataatgcg ttccttcgat accgccagtc
     6601 atcaatttat acacatcttc ttctaaaaga tcatcaacaa attgaagtaa tttcttcaat
     6661 tgtgcttcgt cttttacttg agatttggga aatgctagca agccgccaat accatttcct
     6721 tctgaccaaa catgataacc gccgccttca ccatctgaga tcttgtttac aggcactaat
     6781 tccatatcat cttgaatacc ttgacccatc gtcttcagat tcttgatatc caccattcca
     6841 gtgtagattc cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt ttgggtaact
     6901 gcaaagtctt gtgccaaata gccattctca aatagatctc ttgaaaaatt caaagctttc
     6961 atatactctt ctgtatcaaa ttctggagtg aatttgccat tatcgtctac agcccagcca
     7021 tttggtgctc caaaataaga agtgaaaagt ttaaagctgg tataacgaat atcactaggc
     7081 gcacgatcgc caaaaccaac tgtgtcgtcc acaccatttc catcaggatc gttttctgta
     7141 aattgtttag ctacttcata aagctcatcc atggtagttg gaacatctag tcctagatta
     7201 tccaaccagt cttttcgaat gactaagcca gcacgagcgt agtctttttg tatcggtacg
     7261 ccgtacaagg caccgtcgat actcgcagct tcgattctat cttcagaaat ttgacttaaa
     7321 tttttatagt cttttaaata ttctgataca tcccagaata cgcctgattt taacgcattg
     7381 cggacagaag agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc tagttcatca
     7441 gaagccaatg cagttgttaa tctttcttct tttgaagcat caggaatcca gttgaatgtc
     7501 agttccgtat cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg cggtgaagca
     7561 tgatgcaaaa tatttaacca agtgatttta gcttttccat cttctgtctc tgaagaactg
     7621 ttacttgaac tgctgccgca tgcggcaagt gctagaattc ctgcaaaact aagacctaat
     7681 tttacgagcg ttgctttttt cataaatttt ctccttcact gttttaataa tatttttgtt
     7741 ttcttctgtt ttttctgtat atgagttctt ccattccaaa tggcaaaata tagaatttgc
     7801 tgtccacgtg actgcggaaa agaagagact gccaccgata aaggttaaaa cacctggcat
     7861 tgtttggatg atcatcagat aaatccctac catacttaat atcaacacta atgagtaatg
     7921 aagatgtgag aatccgaata aaaatgccat tttcattttg aaaaaatatc ctttccaatc
     7981 ataatgagcc atgattggga aaatatagag tgctgagagc acatatatga acgcagcaat
     8041 atacaaagcg atcctaagat accaattgtg tatgaaagaa agatccacaa gtaaaacaat
     8101 cccgatggcg ccatatatcc agctcatcag caaggtttca cgatagtttt ctttaaaata
     8161 tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt ttctaatcac
     8221 tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg cgatgactaa
     8281 accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc ttccgtttat
     8341 tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa cgccgtcttc
     8401 tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta cgaccccttt
     8461 aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag ctgtataaat
     8521 ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca agtacatatg
     8581 ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa tgattactgg
     8641 acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc catccatttc
     8701 tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaaggtaga tgatcgtaga
     8761 ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc attcagggga
     8821 tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca atacccctcc
     8881 gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca tgaagtgagg
     8941 gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt catttaatag
     9001 taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga acaagatcaa
     9061 cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa
     9121 accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa aagcaataac
     9181 gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg gcagaatcat
     9241 cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc ttctgagctt
     9301 ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac tttccataag
     9361 cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata actatctgaa
     9421 ttacgtatat aatcattttt atcgtaagta aaaacgctta catagcaatg atttttgtgc
     9481 tatacaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa accttctatt
     9541 tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc accgatgata
     9601 aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct atctatatga
     9661 aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg tttctaattt
     9721 tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct tttgcttcta
     9781 cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct ttagacgcgt
     9841 atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg attggtgtta
     9901 caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac ggtctttcca
     9961 gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct atgttgttca
    10021 gcggggggat gattccaacg ttcttagttg ttcgttctct agggctggtc aattcttact
    10081 gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg agaaactttt
    10141 tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc acagattttg
    10201 gtgtattttt caaaattatg ctgccattag ctttaccgtc tattgctact atctcgcttt
    10261 tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc aacgattcat
    10321 ctaaatggcc gatacagatt cttttacgcc aaatcgtcat tgtatcaagt gggatgcaag
    10381 cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg gctgttatcg
    10441 ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat tttgtcaaag
    10501 gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg gtagttgtgt
    10561 cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc ttaaattttt
    10621 gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta tcagaggttg
    10681 tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg cgacgagtaa
    10741 cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca caaaattatg
    10801 ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa
    10861 gacttttgtc acagttcttt ttgcgtgctt agccttgaaa tttaactatc cataaataca
    10921 aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga aaaacagcct
    10981 cttgattaaa cggcgtttat cttgttgatt ctgcggcaat aaaatcagcc agcagcaaaa
    11041 catgaagtgc tgttttttgc tgtcctcttt tattgcttga atcagatcac aagaaaaaca
    11101 gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat tccttctttt
    11161 taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca atctctttta
    11221 cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga ttttgaacaa
    11281 aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag ttagggttat
    11341 aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct gtgtagacga
    11401 tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct tgagagcatt
    11461 ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg ggatgataat
    11521 tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa tctactccca
    11581 ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact tcccgactca
    11641 aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa ttcttcactt
    11701 cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc ggatatctaa
    11761 taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt gaacttggac
    11821 cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc ttgctttcat
    11881 tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag tattctttta
    11941 cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct gcatcatctt
    12001 tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc agcattcgat
    12061 tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc aaatcacgct
    12121 ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa tagcagtcat
    12181 tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt tctactcgat
    12241 ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta actaagtgtg
    12301 cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc aaatctagtt
    12361 ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga gaaatcggac
    12421 gggcaaatct gtccgaaaat aagtaggaaa aaagaccaat gagtcctacc agtgttaaag
    12481 acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca ctagagtcaa
    12541 cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac gttttatctt
    12601 tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat acagttggtt
    12661 gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt tcaatagaaa
    12721 gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct atcgtagcaa
    12781 ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt tcgattcctt
    12841 tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt tgatactttt
    12901 caacttcctc ttcggtcagt tgactcaacg atccattgat tactccccat ttaccagcca
    12961 aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa tttagttctt
    13021 tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg aatatgtccg
    13081 agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta attttccatt
    13141 caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa ctcgattcga
    13201 tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt acggctccta
    13261 ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt ttcttcctct
    13321 cctcctttta aaataaaagg ctttcatttt tctgtaaaat tgatactcat ctaattaaat
    13381 gagcattccc cgctttcaag aatagcataa ttaaaaataa aaatccaagt aatttttaac
    13441 gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt aatatgatga
    13501 atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga tcaaagatat
    13561 cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata acagcagttt
    13621 aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga attattcgcc
    13681 aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac tatttttctc
    13741 aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag gcatccattc
    13801 agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg atttaacaga
    13861 aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg atgatcagcc
    13921 tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc gtcacatcaa
    13981 tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc aggcagtaga
    14041 ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga aaaattttcg
    14101 ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga gcatccctgt
    14161 caatctagcc tattttttaa caggagatta cagcttagaa agcggcctcc aaaacatgga
    14221 atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg acgacatagc
    14281 gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga acatttctct
    14341 gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta ccaccgtacg
    14401 taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt tgatcgaagg
    14461 caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc gtgagtcgat
    14521 caattacttg taaactacaa gtaattttat ataaaaatgt taacgtgtgc ataacacaga
    14581 aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga agcacctgta
    14641 aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg gcagattcag
    14701 cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc tttagaacaa
    14761 ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt acaaggactc
    14821 agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca aatgatcaat
    14881 ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt ggcctatatc
    14941 gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt aacagatact
    15001 cccccacaga gcttcccggg caaactgaca gcctttttat accgacgttt caaattaaac
    15061 aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga acgtctaaaa
    15121 gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt tgagtggtta
    15181 gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg ttatcctcgc
    15241 gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat ggtcaaagca
    15301 gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca actaccattc
    15361 aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg tgaacgtaag
    15421 gtgcatttgc ttaatggacc gcatacagcg atggtccctc ttgctttgtt ggcggaattg
    15481 gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga tcagttattc
    15541 aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat ttatgctgac
    15601 caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc gatttcatta
    15661 aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta tatcgaagaa
    15721 caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct tatgtatcga
    15781 ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac tgatgcttgg
    15841 tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg gggagaagat
    15901 ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa gatcgatcaa
    15961 aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc ttgaaaccct
    16021 gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa cccctattcc
    16081 aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag atatcccgca
    16141 aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca aatatggcta
    16201 tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca ctcataatgt
    16261 aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc atccaagtca
    16321 ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa aagtagggat
    16381 tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg ctgaactgat
    16441 cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata ttacgatttt
    16501 aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc gagaaatttt
    16561 agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat taggttgtga
    16621 gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag aacgagtgaa
    16681 atttctagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc tattagaaga
    16741 gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta agttgaatgt
    16801 cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc ctttacttgg
    16861 tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag aagtgccaga
    16921 aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag tttttgactc
    16981 aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg aaccagttta
    17041 cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag ataagtcttt
    17101 aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat atggcgaaaa
    17161 aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg tagctgcctc
    17221 tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag ggactccgtt
    17281 tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg aacgcaaagg
    17341 tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag taatcttaga
    17401 atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa atgaacaaaa
    17461 tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt cataaaacag
    17521 tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt aaaagacgat
    17581 tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga ttatcactgt
    17641 cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac agaagcttgg
    17701 ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc tgaagaaaaa
    17761 atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac cgtaccaaaa
    17821 attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt cttcggtatt
    17881 gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa tgaaattttg
    17941 gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga agtgatttgt
    18001 acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca tgaagaacca
    18061 gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga aaaagaagga
    18121 tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac cacctatcaa
    18181 gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg atgtcgtgtt
    18241 tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt attagaagag
    18301 atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga tgcttatctt
    18361 acagaaacac tgatacattt gacccacttc tatcattctc ataattggac gatgcagctg
    18421 cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg gcccgatacc
    18481 ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt attgaaccgt
    18541 gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc gaatgactat
    18601 cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa actgcagtta
    18661 ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt gacacaattt
    18721 gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag aagtttcctg
    18781 tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg cgaaatggtc
    18841 atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca agcgattagt
    18901 tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag taacagtaac
    18961 gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta gagaagaaga
    19021 cggaccaa
//
LOCUS       DARBFD010000052        18740 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000052 DARBFD010000000
VERSION     DARBFD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.052"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            256..531
                     /locus_tag="SCK08_001725"
     CDS             256..531
                     /locus_tag="SCK08_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201600.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="SCK08_001726"
     CDS             544..2616
                     /locus_tag="SCK08_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEG1201601.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="SCK08_001727"
     CDS             2781..4667
                     /locus_tag="SCK08_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1201602.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="SCK08_001728"
     CDS             4679..5626
                     /locus_tag="SCK08_001728"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEG1201603.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="SCK08_001729"
     CDS             5646..6173
                     /locus_tag="SCK08_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1201604.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="SCK08_001730"
     CDS             complement(6236..6889)
                     /locus_tag="SCK08_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEG1201605.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="SCK08_001731"
     CDS             7021..7863
                     /locus_tag="SCK08_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1201606.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="SCK08_001732"
     CDS             8021..8917
                     /locus_tag="SCK08_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1201607.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="SCK08_001733"
     CDS             8920..9633
                     /locus_tag="SCK08_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEG1201608.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="SCK08_001734"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="SCK08_001734"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1201609.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="SCK08_001735"
     CDS             10168..10395
                     /locus_tag="SCK08_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEG1201610.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="SCK08_001736"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="SCK08_001736"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1201611.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11681)
                     /locus_tag="SCK08_001737"
     CDS             complement(11181..11681)
                     /locus_tag="SCK08_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1201612.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="SCK08_001738"
     CDS             complement(11960..12358)
                     /locus_tag="SCK08_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1201613.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="SCK08_001739"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="SCK08_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEG1201614.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="SCK08_001740"
     CDS             13471..14238
                     /locus_tag="SCK08_001740"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEG1201615.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="SCK08_001741"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="SCK08_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEG1201616.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="SCK08_001742"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="SCK08_001742"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEG1201617.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="SCK08_001743"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="SCK08_001743"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEG1201618.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DARBFD010000053        18156 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000053 DARBFD010000000
VERSION     DARBFD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.053"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..588
                     /locus_tag="SCK08_001744"
     CDS             <1..588
                     /locus_tag="SCK08_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEG1201619.1"
                     /translation="TLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGDNVLVDT
                     ILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEIL
                     KKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRH
                     SLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            655..1149
                     /locus_tag="SCK08_001745"
     CDS             655..1149
                     /locus_tag="SCK08_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1201620.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1205..1843
                     /locus_tag="SCK08_001746"
     CDS             1205..1843
                     /locus_tag="SCK08_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201621.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1915..2310
                     /locus_tag="SCK08_001747"
     CDS             1915..2310
                     /locus_tag="SCK08_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1201622.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2294..4747
                     /locus_tag="SCK08_001748"
     CDS             2294..4747
                     /locus_tag="SCK08_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1201623.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4744..6771
                     /locus_tag="SCK08_001749"
     CDS             4744..6771
                     /locus_tag="SCK08_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201624.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6768..7790
                     /locus_tag="SCK08_001750"
     CDS             6768..7790
                     /locus_tag="SCK08_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1201625.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7807..8736
                     /locus_tag="SCK08_001751"
     CDS             7807..8736
                     /locus_tag="SCK08_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1201626.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            8981..9097
                     /locus_tag="SCK08_001752"
     CDS             8981..9097
                     /locus_tag="SCK08_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG1201627.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            9113..9916
                     /locus_tag="SCK08_001753"
     CDS             9113..9916
                     /locus_tag="SCK08_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1201628.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            10151..10387
                     /locus_tag="SCK08_001754"
     CDS             10151..10387
                     /locus_tag="SCK08_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201629.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            10394..12346
                     /locus_tag="SCK08_001755"
     CDS             10394..12346
                     /locus_tag="SCK08_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1201630.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(12418..12915)
                     /gene="dfrG"
                     /locus_tag="SCK08_001756"
     CDS             complement(12418..12915)
                     /gene="dfrG"
                     /locus_tag="SCK08_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1201631.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            13277..14317
                     /locus_tag="SCK08_001757"
     CDS             13277..14317
                     /locus_tag="SCK08_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1201632.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            14415..14603
                     /locus_tag="SCK08_001758"
     CDS             14415..14603
                     /locus_tag="SCK08_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG1201633.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(14661..15014)
                     /locus_tag="SCK08_001759"
     CDS             complement(14661..15014)
                     /locus_tag="SCK08_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201634.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            15545..15967
                     /locus_tag="SCK08_001760"
     CDS             15545..15967
                     /locus_tag="SCK08_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1201635.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            15964..16194
                     /locus_tag="SCK08_001761"
     CDS             15964..16194
                     /locus_tag="SCK08_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201636.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            16692..16892
                     /locus_tag="SCK08_001762"
     CDS             16692..16892
                     /locus_tag="SCK08_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1201637.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            16919..18112
                     /locus_tag="SCK08_001763"
     CDS             16919..18112
                     /locus_tag="SCK08_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1201638.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT         6251 a         2987 c         3451 g         5467 t
ORIGIN      
        1 acgctttgca catatcttaa aataagagtt ccttttgatg atttagactt ttttgaaagt
       61 cataaagatt tgattaccca gacagcaagc tccatatatg ataaacaagg tgataatgta
      121 ttagtggata ctattttagt accacttcca gagaattacg aagtaattaa ctttagtcag
      181 ttgaaaatta gtgatgtggt ttcacaagct attgaagacg ctgagtcttt tatgagtaat
      241 ggtgaatatc aacgtgcctt tgacagagtt cacactgctt ttcatggata tttaattgaa
      301 atactaaaaa aatatgaaat tacagttcct agagacgaaa atctatctaa attatatagt
      361 aggatacaac aattaattga aaaagaaatt caacctactg aacttgctga tatagtgaag
      421 acaacgataa ggtcatcaaa tggaatgatt agctctctta atgaagcaag aaataggcat
      481 tcacttgcac accctaatac aaatattatt ggaaagagag aagcaaaact aataataggt
      541 atttcaagta cagttacaga ttatattagc ggataccttg ataagtagcg tcattaactg
      601 attgctactt tttttatgac tatttttata aaaaggatgt gataagaatt ggaaatgaaa
      661 gtctatgtgg caaatttagg acggtataat gaaggtgaat tagtcggagc atggtttact
      721 ccaccaattg atgaggaaga aatggcagaa agaatcggtt taaatgagga ctacgaagaa
      781 tatgcaatac atgattttga gcttcctttt gatgtggacg aatacacgcc gattagtgaa
      841 atcaaccgtc tatgtgaagc cattcaagaa atcgaaggta cacctattta taatgaactc
      901 aaagaaattc aaggcatgtg gtttagtagc ttggaagaat tactggaaaa caaagaggat
      961 attcattgtt atagtgattg tgattcgatg gaagatgtcg cccgttatta cgtggaagaa
     1021 acaggacagc ttggtgaagt cccttccaat ttacaaaatt atattgacta ccaatctttg
     1081 ggacgtgata tggaaataga gggtaattac ctagttacct ctcatggggt atttgaatat
     1141 tgtcagtaag tttaacaatc cgtgttataa ttaaattaat aaatagcacg gagaggtata
     1201 aattatggta aatgtatttg aaaatcttaa actattaaaa ctagacatga aagataaagg
     1261 ctgggtgata gattcctttt actttcgtta caaacagcaa aattatattg tacttgttaa
     1321 gctatttgaa aaagaggaaa aagttccaga atatgctctg ttaaaactag aatttttaaa
     1381 ggaaaacgat ttttcagata tgctagctgt ctatgctaat tcggtaaaat tatatacaga
     1441 cacgaaaaca ataagagaat attttggtat cgaatacagt agtaacttgg gagatgtttt
     1501 atttcaattt agccaaactc ttgctcgatt tattccgaca gaagttagtg agaagaaaaa
     1561 tgaagaccaa aaagaagcca tgtgtttctc attaagtcaa agtgattcag aagatcccag
     1621 aaaaaaatat tgtttttcag ttagaagaaa tcctttaaag ggaaatggtg agctgggaaa
     1681 acgaagtcct tttaatgaca ataaaacgag attgtataga cccagcttat atgaacgatt
     1741 agggtcagat accaatttaa gtttcagata ttcaatgaat ccagatgatg aagaaactga
     1801 tgaaggtatt attgcaaaat ggacaaaaaa taaaattgaa tagttaaagc attgaaagag
     1861 caatcaccta ttttgattgt tctttttcta tttttacgga aaggacgtga agacatgaaa
     1921 aaaataagaa gttacaccag tatttggtca gtagaaaaag tgatttatgc gattaatgat
     1981 ttacaactcc ctttcccaat tacctttacg caaatgacat ggtttatcgt ttctttgttt
     2041 gtcgtcatac tctttggcag tttaccacca ctttctctga ttgacggagc actactcaaa
     2101 tacttaggga ttcccattgt tctcactggg ttcatgtgcc aaaagacctt tgacggaaag
     2161 aaaccctatg gatttctaaa gtctatcatt acctatgctc tacgccctaa agtaacctac
     2221 gctggcaaac aaatacgatt aaaaaaagaa acaatgaatg aatcgattac gattgtaagg
     2281 agtgaacaac tagatggcat atccgattaa atatatggaa gataatctcg tatttaatca
     2341 tgatggcgaa gtattcgctt attatgaatt aattccctat aattacagtt ttttaagttc
     2401 cgatgaaaaa attcaagttc atgataattt ctgtcaattg attgcccaaa atcgtgatgg
     2461 caagattcat gctctgcaac tagcaaccga atcaagtatt cgagccacac aggaacaatc
     2521 gaaaaaagaa atcactggac gattaaaaga gatagcttac gaaaaaatag atgaacagac
     2581 cgaagcgtta atttctatga ttggtgacaa tcaagtagac tatcgctttt ttattggttt
     2641 taaactctac ctcaatgaac aggaagtatc cgtaaaaaat gttgggaaag aaattcaaaa
     2701 agcatttgcg gaattctttc atggtgtgaa tcacaaatta atgggcgatt ttgtttctat
     2761 gcctatggcg gaaatcgaac gcttttctaa gatggaacag ttgttagaaa gtaaattatc
     2821 cagacgcttt cagattcgta aattggagaa agatgatttt ggttatatta ttgaacatct
     2881 ttacgggcaa acaggtgtcc cttttgatga ctacgactac catttaccga aacgatacga
     2941 agataaaaaa gtgttggtaa agaaatacga tattctcaaa cccactcgct gtttaatcga
     3001 agaaaatcaa cgctatttac ggattgaaca ggaagatacc actacttatg tggcgtattt
     3061 tacaattaat agtattgttg gtgaattgga tttccctaat agtgaaattt tttattacca
     3121 gcaacagcag tttactttcc ccgttgatac cagtatgaat gttgaaattg tcgccaataa
     3181 aaaagcctta tctaccgttc gcaataaaaa gaaagaactg aaagatttag atagccacgc
     3241 atgggaaagc gacaacgaaa ccagtactaa tgtggtggac gctttagaat ctgtcgatga
     3301 attagaaagt aatttagacc aaaccaaaga atctatgtac aaactaagct atgtggtacg
     3361 ggtatcagca aagaatttgg acgagctgaa acaacgttgt gatgaagtaa aagattttta
     3421 tgatgatctg tctatcaaat tagtacgacc ttttggcgat atgctaggct tgcataatga
     3481 atttattccc tcaagcaagc gatatatcaa tgactatatt caatatgtca cgtcggattt
     3541 tttggctggt cttggtttcg gtgctacaca aatgattggt gaaaattctg gtatttacgt
     3601 cggttacaat ctcgatacag gcagaaatat ttatctccaa cctagccttg caagtcaagg
     3661 aattaaaggc tctgtaacaa atgcattagc gtccgctttt attggttcat taggtggcgg
     3721 aaaatcattc tctaacaata tgctggtcta ttattcagta ctctttggcg gacaggcaac
     3781 cattgttgac ccaaaagctg aacgtggtga atggaaagaa accttacctg atattgccca
     3841 tgaaatcaat attgttaatc tcacgagtga gaacgacaat aaagggttac ttgaccctta
     3901 tgtgatttta aaactcccga aagattcaga aagccttgcc attgatattc tcacgttctt
     3961 aacaggtatt tccagccgag atagcgagaa gtttcctgtc ctgcgtaaag ctattcggag
     4021 tgtaactcaa agcaaacaac gagggttgtt attggtgatt gatgaattgc gaaaagaaga
     4081 tacgcccatc agcaatagta ttgctgacca tatcgaaagc tttgtcgatt atgattttgc
     4141 ccatctgtta ttcagtgatg gcagtgtcac ccaatccatt agcttggaaa aacaactcaa
     4201 tattattcaa gtggcagatt tggtcttacc cgattccaat agctcttttg aagaatacac
     4261 taccatggaa ttattaagtg tggcaatgct gattgtcatt agtacctttg ctttagactt
     4321 tatccactct gaccgttcca tttttaaaat tgttgacctt gatgaggctt ggagtttttt
     4381 acaagtggca caaggaaaag ctctatctat gcgattggtt cgagcaggac gtgccatgaa
     4441 tgcaggggtc tatttcgtta ctcaaaacgc ggacgattta ctagatgaaa agatgaagaa
     4501 taatattggc ttgaaatttg cctttcgttc cactgaccta gtggaaatca aaaagacctt
     4561 agaatttttt ggtgtggaca aggaagatga aaacaatcag aaacgcttac gtgaattaga
     4621 aaatggacaa tgtttagttt cagacatcta tggtcgtgta ggggttatgc agtttcaccc
     4681 gatttttgaa gagttgcttc atgcctttga taccagaccg cctgtgcgaa atgaggtgga
     4741 agaatgacac aaaaacaaaa gaaatttctt cactatagtt ggataacgct cctttgtgtg
     4801 ggcgttattt tacttttatt aggcacgctg ggcaatgcag tacaagcgac aggcttagtt
     4861 gatgaaacca ttgatacaag caatgaatac tccaagtatg gtttgaatca atatcagctt
     4921 gattattatg tggacaatag ctggggctgg cttccatgga attggagtga tggcatagga
     4981 caatcggtga tgtatggatt gtatgccatt actaatttta tttggacaat ttctctttat
     5041 ctatcgaacg ccacaggtta tttggtgaaa gaagcctatg ccttagattt cattagtcaa
     5101 acatcccaag caattggcga aaacatgcag acgattgcag gcattacacc gaatggcttt
     5161 tccagttcag ggttttatgt cggtttcctc cttcttttta ttctcattgt tggaatctat
     5221 gttgcctata caggattggt taagcgagaa acgacgaaag tgatacgagc tgttttaaac
     5281 tttgtcgtgg tgtttattct atcggcttcc tttattgcgt atgcacccga ttatattgcg
     5341 aaaatcaatg atttttccaa agatgtcagt accgccagtc tgaatattgg gacaaaaatt
     5401 gttcttccta acacggatag tcaagggaaa gacagtgtgg acatgatacg tgacagctta
     5461 ttctctgttc aagtaaagca accatggcta ttgcttcaat atggctccac tgatgtagaa
     5521 gcgttagggg aagaacgtgt ggaaagttta ttgtctacca gtccagatac aaacaatggt
     5581 gaagatagag aaaatgtagt gattgaagaa attgaagatc atgacaatac ctatttaacc
     5641 ctaccgaaga cgatttccag attaggaaca gtcttctttt tgtttgtatt caatattggt
     5701 atctcgattt ttgttttcct tctaacagga atcatgattt tttcgcaagt gctatttatt
     5761 attttcgcta tgtttttgcc aatcagtttc ctattaagta tgttgccgag ttttgatggc
     5821 atgggcaggc atgccattac caagttattt aatgtgatta tgacaagggc tggtattacc
     5881 ttgattatta ccgttgcctt tagtatttcc actatgcttt attcgctgtc agcaagttct
     5941 ccctttttca tgattatgtt tttacaaatt gtgacctttg cagggattta ttttaaactg
     6001 ggcgatttaa tgagcctatt cactttacaa agcaacgact ctcaaaacat gggcagacaa
     6061 gtgtttcgca gacctcgtca aatgatgaat cgacaaacca gaagatttca acgcagtatg
     6121 agtaagattt ttcgtggaaa agcagggtca acgaagcaaa agacaatgac aacaaagcag
     6181 aatcaaacca ataaagctaa ccatactcgt caaaatgaac ggactacacc aaagaaagaa
     6241 acagcacttt ccaatgtgaa aaagcaacaa ggcggtatcg ggcaacgatt aggtgaaaag
     6301 acagcccatg tattggacac gaaaaatcgt ctagtcaata aaagaaagca ggcaaaacaa
     6361 cagatacagg cgacaccaac ccatgtcaaa tatgcccttc atcaaggaaa agagaatgtc
     6421 actcataatg taacagactt taaacaatct atggcagata ccaaagctca acgcaaacaa
     6481 cagcgtatga accagcagga gcaaaaacga aagaacattg ctcaaaagcg tttggagctg
     6541 gataaagcaa aagaaaaaag acgaaaagaa aacccatcat ctcctgttca gaataaccag
     6601 cagcatgttc gcaagcgtca gcaaaccgtc acgcctgttt tgtcacaaaa cactaaacgt
     6661 gaaaatcatc agcgaccatt gaaaaaagag caaaaacaaa agacaccacc gaagcaattc
     6721 aatcagagga agctaaataa aagaacggat aagcgaggac aacaaacatg aagctaaaaa
     6781 taacagtgat ttgtagtgtg gtcttctctc tcttcttttt tctcttatta ttcctagtca
     6841 ttttctttgc agatgatacg gacagtggcg aaaacaacaa ggattcttct attccacaag
     6901 gcggtgtgac cgtttcacca gaagtgttgg cacaccgtcc gttgattgaa aaatatggta
     6961 aggaatatgg gattgaagac tatgtttctt atatcttagc cattatgcaa gtggaatcgg
     7021 gcggtacagc agaagatgtt atgcaaagtt ccgaatcttt gggtttaccg cccaacagtt
     7081 tgagtaccga agagtccatc aaacaagggg ttaaatattt cagtgaatta ttaaccagtg
     7141 cagaacaaca aggagtggat atagattctg ttatccaatc ttataattat ggcggtggct
     7201 ttttaaatta tgtgaggagt catgggaaaa aatataccta tgaattagcc gaacagtttt
     7261 ctaaagagaa gtcgggtggt caaaaagcag actatcccaa tcctatagcc atacctgtga
     7321 atggtggttg gcgatataac tatggaaatc agttttatgt tcagttggtt tcacaatacc
     7381 taaccgacac ttccccaaca gaatttgatg atgaaaccgt gcaagtcatt atggacgaag
     7441 ccttgaaata cgaagggttt ccctatgtct ttggaggggc gtcgcctacc acctcttttg
     7501 attgtagtgg cttaatacaa tgggtgtatg acaaagctgg catttctctc cctcgtgtcg
     7561 cccaagatca gtatgacgct acgcaagaaa tctctatgga agaagcccaa gcaggcgacc
     7621 ttatcttctt tcattcaacc tataatgcag gcacatatgt gactcatgtg gcgatatatt
     7681 tagagggaaa tcgcttttat catgcaggcg acccaatcgg ttatggcgac ttaagcagtc
     7741 gttattggca agaccatctg attggggcta gacgtgttat tcataactaa gaaaggaatg
     7801 aatcaaatga ttataaaaat cgagcgaaaa gaaaagaagg agaaaccaaa gaaatctaaa
     7861 aaaccgagca agcaaagaaa agttccgatg attaaagttg gcacgcataa gaaattaacc
     7921 tttgttttat gggttttatt gattggtagt gtcggatttg gaatctataa aaattttact
     7981 gccattgaca ctcatactgt ccgtgaaaca gaaatcatta aacagcagat tgtcgatacc
     8041 aaccaagtag aaagttttgt gaaatccttt gcgaaagact acttttcgtg gcaacaatcc
     8101 caagaagcca ttgataaacg aaatgaaaaa ttgacccatt atttgacgga agaattgcag
     8161 gtattaaatg aagaaatgat tcgtaaggat attcctacta gttctagtgt gaacgatata
     8221 caggtgtggc aggtttctca agtgaatgag aacacctttg aggtactgtt ttctgttgaa
     8281 caagttatta ccgaagacaa agacaaggaa actatatcgt ctagctttca tgtggttgtc
     8341 catatagatg aatcagataa tatggttatt atcaaaaatc cgacaatgag taagaaacct
     8401 caaaaatcgg actaccaacc caaacagcta gaaagtgacc atacagtaga tacagaaacg
     8461 atggacgaaa tcactagctt tttagaaacg ttcttccagc tctatcctac cgccacagaa
     8521 aaagaattga cttactatgt aagtaatcat gtgttaccta tgattaacaa ggaatatgtt
     8581 ttcgaggaat tggtgaatcc tatctttacc agaaaagata accaagtaat cgtgaatgtc
     8641 gctgtgaaat atttagatca agaaacaaag gcaacccaaa tctcgcaatt tgagttgata
     8701 ttagaaaaac aagataattg gaagattgta aaataacaaa tattggtaca tgattacaga
     8761 tactttgtaa tcatgtactc tttttgataa aaaattggag attcctttac aaatatgctc
     8821 ttacgtgcta ttatttaagt gactatttaa aaggagttaa taaatatgcg gcaaggtatt
     8881 cttaaataaa ctgtcaattt gatagcggga acaaataatt agatgtcctt ttttaggagg
     8941 gcttagtttt ttgtacccag tttaagaata cctttatcat gtgattctaa agtatccaga
     9001 gaatatctgt atgctttgta tacctatggt tatgcataaa aatcccagtg ataaaagtat
     9061 ttatcactgg gatttttatg cccttttggg tttttgaatg gaggaaaatc acatgaaaat
     9121 tattaatatt ggagttttag ctcatgttga tgcaggaaaa actaccttaa cagaaagctt
     9181 attatataac agtggagcga ttacagaatt aggaagcgtg gacaaaggta caacgaggac
     9241 ggataatacg cttttagaac gtcagagagg aattacaatt cagacaggaa taacctcttt
     9301 tcagtgggaa aatacgaagg tgaacatcat agacacgcca ggacatatgg atttcttagc
     9361 agaagtatat cgttcattat cagttttaga tggggcaatt ctactgattt ctgcaaaaga
     9421 tggcgtacaa gcacaaactc gtatattatt tcatgcactt aggaaaatgg ggattcccac
     9481 aatctttttt atcaataaga ttgaccaaaa tggaattgat ttatcaacgg tttatcagga
     9541 tattaaagag aaactttctg ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa
     9601 tatgtgtgtg acgaacttta ccgaatctga acaatgggat acggtaatag agggaaacga
     9661 tgacctttta gagaaatata tgtccggtaa atcattagaa gcattggaac tcgaacaaga
     9721 ggaaagcata agatttcaga attgttctct gttccctctt tatcatggaa gtgcaaaaag
     9781 taatataggg attgataacc ttatagaagt tattactaat aaattttatt catcaacaca
     9841 tcgaggtccg tctgaacttt gcggaaatgt tttcaaaatt gaatatacaa aaaaaagaca
     9901 acgtcttgca tattaagtag ttcagttttg gagtacaaaa agtgaaaatg taaacctaaa
     9961 tgagtatcaa attgcaactg aatttgatac tcgtttttta ttgttaaatc aacgtttgtt
    10021 gttaatttac accacaaaat gtaagttaaa atgtatataa aaatgcgaac gtgaaataat
    10081 tattgtagga atgcaaattg gtgtgttaaa atggttacaa tttactaagc aaaaaggggt
    10141 gcattttcct atgcaatttc ttgagccaaa aaataagaac gctaagtctg tggattggga
    10201 aatatcagag caagtaaggg tgatagttaa gcaatacgct gaatatgcag agcgtactga
    10261 aagcgaagca gtggatgaat tcttattgaa cattttagac gataagaagt ttattgaatg
    10321 gattgccaac aagcgaagta ataagcggat tgttgaaaaa atgggtatca aagatagagt
    10381 gggttgatag acaatgacca agcttaaaag attagccacg aaggaagatg aaattgtaga
    10441 tgttaaaatt ccgatttcaa acgaagaatt aaaagatagg gctaaacaat atgatttatt
    10501 aactccaaaa cgatttgcaa caaggtataa caaaatgttg ttcttgccaa cgtcttttaa
    10561 atggaacggt tctgaatatc ccattcaata taattactgc atcaatccgt tttgttgtaa
    10621 ttttgggaaa gaacagcaca aattcaaaga tgtaaaaggg aaaccaagtc gatataaaat
    10681 gactggtagt tccaaagata aaggtcataa aggtatgtac tgcaatgaca atcctattgg
    10741 cagaggtgta tcgcaaaact gtacagtaac ccctttatct aattggtctg ttgttgaaga
    10801 aattaaacgc cttatagaaa tcaatagcat ccaagatgtt gagccagatt atcaatttca
    10861 taaagaaggc tgctcggaag aagaatcaac cccattcaat gagccgaagc aattttacaa
    10921 aagggggaaa agtaggggca aatctcaacg ctatcaatgt aaggcgtgta agaagttcac
    10981 taatgttcta ccaaaaagag aagaaaccac tacttatcac caacaaaaga acaccattct
    11041 tccaatgttc gctaagatgg ttgttggaag ggtttcagtc agtcgtactt gtgatatttt
    11101 aggaattgga gttggaacct actatcataa gctggagtgg ctgtataggc gttgtttgga
    11161 gtttttagaa cgatatgaga cacaacccct tcaaacaaag aaatttaacg agatgtggct
    11221 taatacggac aagatgcact actacctaaa taacgtccgt aagaaaggac aaggttcaaa
    11281 gaaatatacg gggtttgaag accttaatat gcaaacgtat atagttgtat ctgctgaggt
    11341 cttatcaaga tatgtgttcc gttctgatgt tgcctatgat tggaatattt caatggatga
    11401 attaaatgaa gatacaagaa aattcaaaga agaccatctc aatactttta gtcgtaaaaa
    11461 tgataggtta gattggtctt attatccaca agaaccatcc gctaacgata gcgaaaatag
    11521 gaatgcttat cttcacgaat tagggaaaat tactaatagg agtagatttg tcgatggttt
    11581 aaatgttgat gctccttata ctacaactgc tcactattgg ttaattaaac aaatggttaa
    11641 tgccgacgaa tggcgaatga ttagtgatga tgacttctct attagaaacg ccttttatag
    11701 agtattcacg aaagaattaa ggttgagcga tgcacatcat tttatttgcc aagttaacaa
    11761 gacgaaaagc agaaaacaat gcttaaaaga attcggacaa gcaaaagcag aactgctcga
    11821 ttggggagat atcagaggat tcaaaacaaa gtttctacgg actttggcat ctcattatct
    11881 aactgaatta ctaacaagtc atcaattcca cgaagaggct attagtaaag atggagaaag
    11941 atatcgcaaa tatgccgata accctatcaa acatccatta gccactaagg ataaaggatt
    12001 ttattcagtt gattgtagaa cggatttatc tgctctggaa ccaaacgaaa tcgcaaagat
    12061 gctattaaat gttaatgacc attcgaccaa tagctttatt caacagataa gacgttatat
    12121 atcatctctt gaaagaccat taacaacagc tcgtggtgat aagaagagct atatttacgc
    12181 taacttcaat ccgaagtatg ctcaatttgc gattacaatt ctaagaactt actacaactt
    12241 ttgtcgccct tttaaatcag cggataaaaa ggttttgaca cctgctcaaa gattagggat
    12301 tacagataaa caatttgatt ggaaagatat aatttatttc aaatgacaaa aagagcttgg
    12361 agtaatccca agctcttgtg ctttatcttt actgttcttc ttcaattaac gcacccgtta
    12421 actcaataag ttttttcttt catatacatg aaaatagtag ttatacggat ttttatcatt
    12481 ctttatccct ttttgggcaa atacctcatt ccattcctca taattcactt ctggaaaaaa
    12541 agtatctcct tcgaattcat gatgtatttt tgtgatgtac attttctcaa cataagggaa
    12601 aaacaaatta taaatctgtt ctcctccgaa aataaaaatt tcttcttcgt ttttacataa
    12661 ctcaaaaaca tcttctattg aatgaacaat ttcacaacca ttaaaggtaa accccttatc
    12721 tctcgtcaga ataatatttc ttctgtcagg taaggctctt ccgattgatt caaggttctt
    12781 cctacctaat attatcggat gtccctttgt agtattttta acatattccc agtccttggg
    12841 aatcctccaa ggaatgtcat tctctttgcc aatcactcta ttcttatcca tcgcagcaat
    12901 caaagaaact ttcatttata acacagcctt tttaactaat ttaccatcta atttcttatt
    12961 tctatcaaaa aaccttttct cattatcctg cccgttagtt gaataagcaa ttttttctat
    13021 aagttatatt atcataatac cacaaaatca caaattttca ttcaattatc attttcaatt
    13081 gcattttata aatcattttg tatgtaattt ggtatgtaag ttaaaatgaa aaaagtagtc
    13141 cattttgaag tacattttgt cattttgtac tccaaaactg aactacttac ttgcatatat
    13201 acgcctttat agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa
    13261 aataaaagtt acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc
    13321 ttattctgga gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg
    13381 agatacaaaa ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac
    13441 aactgttgaa ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat
    13501 ctcagatagt gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact
    13561 ttctttctta gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca
    13621 tgtggagata gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc
    13681 agaatatacc attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc
    13741 tgtatcaccg cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata
    13801 cttaaatcaa tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg
    13861 attatatggt tggaatgtga cggattgtaa aatctgtttt aagtacggtt tatactatag
    13921 ccctgttagt actccagcag attttcggat gcttactcct attgtactgg agcaagcctt
    13981 tagaaaagct ggaacagaat tgttagagcc atatcttagt tttaaagttt atgcaccaca
    14041 ggaatatctt tcacgggcat ataacgatgc tcccaaatat tgtgcaaata tcgtaaatac
    14101 tcaactgaaa aataatgagg tcattattat tggagaaatt cctgctcgat gtattcaaga
    14161 ttatcgcaat gatttaactt tttttacaaa tgggcttagt gtttgtttag cagagctaaa
    14221 aggatatcag gttaccactg gcgaacctgt ttgccagacc cgtcgtctaa atagtcggat
    14281 agataaagta agatatatgt tcaataaaat aacttagtgc gttttatgtt gttatataaa
    14341 tatggtttct tattaaataa gatgaaatat tctttaatat agatttgaat taaagtggaa
    14401 aggaggagat tgttattata aactacaagt ggatattgtg tcctatttgt ggaaataaaa
    14461 caagactacg aatacgagtg gatactatac ttaaaaattt ccctttatac tgccccaaat
    14521 gtaagaacga aactttaatt aatgttcaaa aaatgaatat aataacaatc aaagagccag
    14581 acgccaagac gcagagccga taatttgaga aatgaaactc tcatcttatc ggctcttttt
    14641 gtttatctga attttactga ctagccttca atatttcttg cttcattgat accttttgct
    14701 agtgcttcca ttaaggatag ttctttgtct gtaaagctat ccatgtattt ttctacctgt
    14761 aaccttcgag tgcttttaat caaattatct gtaggtaaga aaaattcatc aatagataca
    14821 tggagtaatg atacaagatc atacagcact tgtgtacttg ggtgttgtcc tttgttttct
    14881 atatttgtta aataacgtgg gtcaatttca atcattgttc ccacttgttc acgagtgaga
    14941 ccccgtttca ttcgagcctc tttaatcgct aaaccaacgg ctctaaaatc gtatttatct
    15001 tcttttttac gcatactaaa tcacctctat acattttact gttcctatat aaccataaac
    15061 aggtatagaa aaacatgtta tatagtttat gagttcctat agaacactgt ggaatttatt
    15121 aatgcgatga atccaatttc aaatagaaag gtcgctgatt ttatttacct atacaacttt
    15181 ataaaattta gtcatactcc tagaaagtag ataaaattgg cgacttaatg gagggaaaag
    15241 tcgccatgag aaagataatc tgtcatctag ttctcttaat taagttctta tcaaaaaaac
    15301 ttatccacct gtactggata gatatgacca tcaatgtggt tttgcagatt acatagatag
    15361 aaaaatcaaa gcatatgaac agagaaaaca atctgtttat gtgctttttt ggtcacttaa
    15421 taaatatctt ttaggatttc tcttcattta tgcaacaaat cccccgttcg tgtcggggtt
    15481 tagtatgaag ggagataaag aagcacatgc cactcccttt cacgacagaa agggggtgaa
    15541 aagcatgaaa ccatcttctt ttcaagaaac catagaaaat cagtttgact atatctgcaa
    15601 acaagttatt gaagatgaac gaaaagatta cttcaaacat ctagacagac tatcagcaaa
    15661 agaagtctcg ttttctgata tagataacta ccttgtcaac tctttttcta ctgttgatac
    15721 gtacgtcact gatttccaac tatttaaatt atttggatta agaatcaatg ttgaaagtga
    15781 tttattaagt gaagccctgc gtcatctttc agagaaaaaa cggaacatta ttttacttca
    15841 ttactttatg gatatgagtg atgtggagat tgcggagtta ctaaatttga atcgttcgac
    15901 tgtttatcgt catcgaataa gtggactagc catgattaaa gaattcatga aggagtgtaa
    15961 agaatgaaaa cagcttatcc aagggttccc ttccccctca ttgtaaaggc gacagatggc
    16021 gacgtggaag caattaatca gattgttaaa cattatcgag ggtatacctc aaaacgttca
    16081 ttacgtcgca tgacagacga atatggaaat tctcatatgg ttattgatga aaccctacgt
    16141 ggcagaatgg aaacgaaact catctctaag attttatcgt tcgaaattaa atagtcccat
    16201 tttttcgtgg aagcgtgtcg aatcgtatcc acgcttcccg atttgggagg tttgttattc
    16261 catcaaagca tattgtgtgt tcagtaggtt ttgataggct aacaagccaa acgttctttg
    16321 acaaccaaat aaaggcaatc gaatacgttt cggtaagaaa agagctgatg aactaacacg
    16381 ccatgaccta caaaattagt gagcgattca tgttagtaat tcgaaagtaa tctttagcag
    16441 gattgctgcc atgactttct tatcgtgata atgatactcc cgtatagcca tagtccgagc
    16501 gttagaagcg tcacaggcaa tgggtacggc tacatgagaa ccatgcagaa gtgaaattcc
    16561 tgtgagcttt gctagagctg ttcgattgct aagatattct tctcttgtca gtgtattatt
    16621 ataaaactat ttgatataat tataggtgaa tattggagtt ttcctagcct ataattggga
    16681 aaggagtaat aatgataacg gtcacaaaaa aggatttagt tgagctagga tatggaactt
    16741 ctttttcagc agacatcata aaaaaagcaa aagaattgat gattacaaaa gggcatatct
    16801 actatcaatc tcgaaagcta gatagagtcc ctcgtgaagc tgttgaagaa attctaggaa
    16861 ttcggttttc aaatgaaaac aggtctgagt agtttttgca cagtaaggag taaaaactat
    16921 ggttaaagac ccaataaaaa aagcaaagaa tggaacctat tattttcgtg caaatctagg
    16981 ttatgattct aaagggaaaa aaattcaaaa atatcgtagt ggattcacta ctaaaaaaga
    17041 agcaagagaa gaatattcga aactgctatt gatgaaacca gaagaattaa gtgagaataa
    17101 ggataaaatg acgtttaaac attatatctt tgaaattttc cttccatggt ataagacaca
    17161 ggtaaagctc agaacatatg aaaatcgctt accaactatt aagaagcatt tctcttattt
    17221 tgataaaatg gctgtttccg atattgagcc tattgatgtg caaaattggc aactaaaatt
    17281 atcaaagaag tgtaagtctt cttatgtaag agctgttcaa ggactatttt cagttgctat
    17341 ggatcgagca attgtccttg ggataaccac cacaaatcca tctaagatta ttggcaatgt
    17401 gaaaaagcaa aagtctaaaa ttgaattttg gacaaaagaa gaatttgaaa aagtaatctc
    17461 tctaatttac aaagaagatt actatcaaca ctttttattt atctctctgt ggtttttatt
    17521 tatgactgga atgcgaattg gtgaagctac tgccattcaa tgggaagata ttgattttga
    17581 ctcaggagta ctaaccatca acaaaacact ctattacaag aatcaatcga attatagatt
    17641 tgttgaacca aaaacaaagg cgagcgttcg tcatattgta ttagataaat gcacattaac
    17701 ttatttatcc gaatggaagg aagtacaaca aaatctaatt cagacagact ttgtaatgag
    17761 ctataacggt attcctaccc aaaaacatac actatcatat gctattgaac gctatgctaa
    17821 gctggctggt atccatcgaa tcagaatcca tgggttacgg cattctcatg cttccttgtt
    17881 aatcagtatg ggagaaaatc cacttatcat taaagatcgt ctaggacacg aagatattga
    17941 gacgacttta ggaacgtatg gacatcttta tcctaacagt aatttcgagg tagctaacaa
    18001 attaaatgga gccatttcct ttaaagaagc aaaacaaaat tatgatactt caccaaagaa
    18061 tcaatttact gtagactact tgcgaaacaa agaaatgaaa aaagtgcaat aaaagtgcag
    18121 taaaaagata agaagagcca gaaacccttg gtataa
//
LOCUS       DARBFD010000054        16573 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000054 DARBFD010000000
VERSION     DARBFD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.054"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(29..727)
                     /locus_tag="SCK08_001764"
     CDS             complement(29..727)
                     /locus_tag="SCK08_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEG1201639.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="SCK08_001765"
     CDS             complement(720..2072)
                     /locus_tag="SCK08_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEG1201640.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="SCK08_001766"
     CDS             complement(2295..3695)
                     /locus_tag="SCK08_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEG1201641.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="SCK08_001767"
     CDS             3856..4593
                     /locus_tag="SCK08_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEG1201642.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="SCK08_001768"
     CDS             4718..5197
                     /locus_tag="SCK08_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEG1201643.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="SCK08_001769"
     CDS             5194..5961
                     /locus_tag="SCK08_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201644.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="SCK08_001770"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="SCK08_001770"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1201645.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="SCK08_001771"
     CDS             complement(6864..7466)
                     /locus_tag="SCK08_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1201646.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..8041
                     /locus_tag="SCK08_001772"
                     /pseudo
     CDS             7572..8041
                     /locus_tag="SCK08_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8147..8731
                     /locus_tag="SCK08_001773"
     CDS             8147..8731
                     /locus_tag="SCK08_001773"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG1201647.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="SCK08_001774"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="SCK08_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEG1201648.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="SCK08_001775"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="SCK08_001775"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEG1201649.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="SCK08_001776"
     CDS             10632..10811
                     /locus_tag="SCK08_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201650.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="SCK08_001777"
     CDS             10833..11858
                     /locus_tag="SCK08_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEG1201651.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /locus_tag="SCK08_001778"
     CDS             12033..13277
                     /locus_tag="SCK08_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEG1201652.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="SCK08_001779"
     CDS             13430..14377
                     /locus_tag="SCK08_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1201653.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="SCK08_001780"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="SCK08_001780"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEG1201654.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="SCK08_001781"
     CDS             complement(15279..15521)
                     /locus_tag="SCK08_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201655.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="SCK08_001782"
     tRNA            15642..15714
                     /locus_tag="SCK08_001782"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="SCK08_001783"
     CDS             complement(15833..16471)
                     /locus_tag="SCK08_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1201656.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5650 a         2951 c         3331 g         4641 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa ttt
//
LOCUS       DARBFD010000055        16423 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000055 DARBFD010000000
VERSION     DARBFD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.055"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="SCK08_001784"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="SCK08_001784"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEG1201657.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="SCK08_001785"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="SCK08_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEG1201658.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="SCK08_001786"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="SCK08_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEG1201659.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="SCK08_001787"
     CDS             2857..3732
                     /locus_tag="SCK08_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG1201660.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SCK08_001788"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SCK08_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEG1201661.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="SCK08_001789"
     CDS             4629..5063
                     /locus_tag="SCK08_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEG1201662.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="SCK08_001790"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="SCK08_001790"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEG1201663.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="SCK08_001791"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="SCK08_001791"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEG1201664.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="SCK08_001792"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="SCK08_001792"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEG1201665.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="SCK08_001793"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="SCK08_001793"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEG1201666.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="SCK08_001794"
     CDS             13334..14260
                     /locus_tag="SCK08_001794"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEG1201667.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="SCK08_001795"
     CDS             14442..15335
                     /locus_tag="SCK08_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEG1201668.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="SCK08_001796"
     CDS             15469..15594
                     /locus_tag="SCK08_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1201669.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="SCK08_001797"
     CDS             complement(15728..16360)
                     /locus_tag="SCK08_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201670.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5838 a         2793 c         3500 g         4292 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcg
//
LOCUS       DARBFD010000056        16382 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000056 DARBFD010000000
VERSION     DARBFD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.056"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            135..2606
                     /locus_tag="SCK08_001798"
     CDS             135..2606
                     /locus_tag="SCK08_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1201671.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="SCK08_001799"
     CDS             complement(2709..4445)
                     /locus_tag="SCK08_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEG1201672.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="SCK08_001800"
     CDS             complement(4442..5146)
                     /locus_tag="SCK08_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1201673.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="SCK08_001801"
     CDS             5277..5780
                     /locus_tag="SCK08_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEG1201674.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="SCK08_001802"
     CDS             5740..6078
                     /locus_tag="SCK08_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEG1201675.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="SCK08_001803"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="SCK08_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEG1201676.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="SCK08_001804"
     CDS             7629..8207
                     /locus_tag="SCK08_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEG1201677.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="SCK08_001805"
     CDS             8211..8732
                     /locus_tag="SCK08_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1201678.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="SCK08_001806"
     CDS             complement(8811..9365)
                     /locus_tag="SCK08_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1201679.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="SCK08_001807"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="SCK08_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEG1201680.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="SCK08_001808"
     CDS             9905..10156
                     /locus_tag="SCK08_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEG1201681.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="SCK08_001809"
     CDS             10281..11072
                     /locus_tag="SCK08_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201682.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="SCK08_001810"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="SCK08_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEG1201683.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="SCK08_001811"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="SCK08_001811"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEG1201684.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..12997
                     /gene="rplS"
                     /locus_tag="SCK08_001812"
     CDS             12650..12997
                     /gene="rplS"
                     /locus_tag="SCK08_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEG1201685.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13129..14265)
                     /locus_tag="SCK08_001813"
     CDS             complement(13129..14265)
                     /locus_tag="SCK08_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1201686.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14381..15295)
                     /locus_tag="SCK08_001814"
     CDS             complement(14381..15295)
                     /locus_tag="SCK08_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG1201687.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15386..15814)
                     /locus_tag="SCK08_001815"
     CDS             complement(15386..15814)
                     /locus_tag="SCK08_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1201688.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15819..16193)
                     /locus_tag="SCK08_001816"
     CDS             complement(15819..16193)
                     /locus_tag="SCK08_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1201689.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         5281 a         2952 c         3220 g         4929 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    12961 cggaaaagct gctcgtatca aagaaatccg tcgttaatca tcgttatcag actgagaaag
    13021 aacccttggt attcaagggt tctttttttg ttctctgttt ttttatatta acgatttcta
    13081 tttgggacat ttttgattaa taaaaagggg caaaaaaggg gcaacaagtt acagtttttc
    13141 tatatcttct ttcaattttt cttttactcc ctttgtcaca tgcaaataaa tcttttcagt
    13201 gatatcacta ttttcgtgcc caacgcgatc ctgaatagca tacagaggag ttcctagttc
    13261 cgctaatttt gaaatatggg tatgtctaaa aatatgtgaa ctaagttttt tatcaatctt
    13321 catgtcagct ttatgatttc tcaaataggt gttaattgct gttagttgga aaggagttcc
    13381 tttcgttgtt tgaaatagaa attgaccatt cggatttaat tctagaagct cattgtaaat
    13441 agctatcgct ttttttggta aatcaatttc tcgcattcca gcagcagttt ttgtagaatc
    13501 agattttttc atatcagcta ttgaacgttc tctatacatc atcgtcccat ttataactac
    13561 tgatgcatta ttattggtaa tgtgtacatc gtctttactt aatgcgattg cttctccagg
    13621 cctcattcca gtcaaatata gccactggaa aaggagagaa tatcttttat tgtgtgaagt
    13681 tgtgaaattt actagtcttt tatattcatc atcttctaaa aacttatctt taatttttat
    13741 cgtttttgac tctcgtttat aatcaataac tacttcgttg ataggatttt tttctacata
    13801 gccttttttc atagcgtatg aaaaaagaag attcaatttg gatttaatta cactgacgta
    13861 cttatttgac aagtcatctt tatatatcat atcttcaaaa atattattta aatctactgt
    13921 attaatacca gaaacgatgt aagtctcagg tatctttttc tttatagtgt ttaagatatt
    13981 attcgtagga taatatgtag actccttaac ttgtcttttg taaataacta accattcttc
    14041 gacaagctca tgaaatgtta gatcaggctt ctgaagtgtt ttttcgttta attttatatc
    14101 aattttttta tttaactcta gcaatgccac tttctgagtt tctcgagatt tatttttata
    14161 agtaatgctc actttttttc tttttctagt ttttggatca atatatcgct cattatattt
    14221 atatactttt tttccgtttt tatcagtttt ggtttcaatc cacattgttt tcatctccta
    14281 tttgttgcta taataggaat ggataagtaa gccaattata gcaggtttat ttttcatcac
    14341 gtccacaaac tttggcgagg gagggggcgt gtttttttgt ttagtagcta cctttccatg
    14401 aagtatctgc gaagaacact tcaaatgttg ttccatcatt tttcacttca aaatatgctg
    14461 ttccttgtgc ggatttaccc ggttggatgg tttccgaaaa gtaatctcta ctagctaaat
    14521 cagatttaat atctgatgaa tcataaaatt ctaaataact tgaacttgca tcaaaaggtg
    14581 tagtccctgt gttttctaca agaaaatcta cctttgcgta aaataggcct tctggtttat
    14641 agaaatcatc accgtaatct tttgatacag actggacagt tacgttaata gagttaccat
    14701 tttcagaagt aaaaggaact gcttctccta tactatgtgc aactgatgcc gtctggggtt
    14761 ctgaagacga agttgtagtt gtagttgtag tagagcttga atctttggat tttccatctt
    14821 tatctaagaa agttcgttga ttataatata caacctgttc agtttttgga ctaacccaaa
    14881 ttaacattgt ttcatcatca gaatcagttg aataagtata ttggagcatc tctaattttt
    14941 ttatatcttt tgcactattt ccatgttcaa ttaattcaga gtgctcttta taataatctc
    15001 cttgtgcatc ctttgaaata gatacaatta attcatcccg tgtgatagca ttgtctaatt
    15061 ctttgtaaac aaaatcattt gtccatttac gtaacggtga accaagtgtc tgataaactt
    15121 gagttaaatc atcaccaact tttattttat gaacgtctga tgaagttagt ttttttgttt
    15181 catattcaga tgttgatgaa acagtatttt ctttagtgct agcagaatta ggactagtgg
    15241 agcatccgct taataccaag cctaaaaaaa gcaacccaaa aacaattttt ttcattttta
    15301 attcctcact tctatgatat gatttttttg taggatctta gaaataaggt ttttagtccg
    15361 tgttgcagca cggacttttt ttacattata taagaaagcg ataagcgctt tctggaagtc
    15421 cgtaaagatt ctttaattcc tcaattcgtt taggatattg atcattgtct tctttgtata
    15481 gagaaacaat gagattagca gcaaagcaat tagcttcgct ttcagatttg cttctagatg
    15541 ttcttgttga tacatagtaa ctggataagc cacgatgaaa aatagcgtga cctaattcgt
    15601 gagcgcaaat gtagaatctt tcctcagagt ctctcagttc atcatttaag aagattattg
    15661 cacgacctct aatttcttga aactgtcctt tgggattctc gataaaagga acgtattgaa
    15721 ttttaatgcc catcttttca caaatataaa aaggattagc ggactggtat ttccgcttca
    15781 actcctcgac taaattaatc gtatccatct ccataagctc acatcttttt gcctttttct
    15841 ttgtcttctt tcacaatatc ccagaaggtt ccaataagga tatcttttac gcgttggacc
    15901 tgttcaggcg tcagtgtttc tccgccataa gacatattga catttgaatc tagtaattta
    15961 tcaagttcaa ctacttcatc ttttgttgcc cagtcaggaa cttggtttct accgagtaaa
    16021 taatcagtag ttacattaaa ataatctgct acaagttgta gtcttttagt actaggagtt
    16081 ctatttttcc attgataaat cgtgttttta ggtatattta attcttcttc caattgagtt
    16141 atacttatgc ctcttttatg agctaattcc tttattctat ctagtaaatt cattttcgca
    16201 cctcacaagc tacgaaaaca ctaataaaaa aattagttaa aggtgttgac aactaataaa
    16261 agtgttagta tactgttttc gtaagctaaa atattagcta aaaagacttg ataactaata
    16321 aacacttcac ggtcggcaaa ctctgaaatg taaattacta ggcatttctg tgtcttattt
    16381 ag
//
LOCUS       DARBFD010000057        16158 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000057 DARBFD010000000
VERSION     DARBFD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.057"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            226..894
                     /locus_tag="SCK08_001817"
     CDS             226..894
                     /locus_tag="SCK08_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEG1201690.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="SCK08_001818"
     CDS             complement(1094..1480)
                     /locus_tag="SCK08_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201691.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="SCK08_001819"
     CDS             complement(1452..2834)
                     /locus_tag="SCK08_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEG1201692.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="SCK08_001820"
     CDS             complement(2990..4177)
                     /locus_tag="SCK08_001820"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEG1201693.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="SCK08_001821"
     CDS             complement(4184..5215)
                     /locus_tag="SCK08_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1201694.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="SCK08_001822"
     CDS             complement(5231..6685)
                     /locus_tag="SCK08_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1201695.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="SCK08_001823"
     CDS             6860..7765
                     /locus_tag="SCK08_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1201696.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="SCK08_001824"
     CDS             7856..8533
                     /locus_tag="SCK08_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1201697.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="SCK08_001825"
     CDS             8530..8700
                     /locus_tag="SCK08_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201698.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="SCK08_001826"
     CDS             8814..9710
                     /locus_tag="SCK08_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1201699.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="SCK08_001827"
     CDS             complement(9765..11135)
                     /locus_tag="SCK08_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1201700.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="SCK08_001828"
     CDS             11373..12119
                     /locus_tag="SCK08_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1201701.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="SCK08_001829"
     CDS             12116..12955
                     /locus_tag="SCK08_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1201702.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="SCK08_001830"
     CDS             12956..13906
                     /locus_tag="SCK08_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1201703.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="SCK08_001831"
     CDS             14047..14799
                     /locus_tag="SCK08_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201704.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="SCK08_001832"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="SCK08_001832"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEG1201705.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5246 a         3016 c         3242 g         4654 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatggat tattgcagtg aatgttacag aatctgcgtt tttcattcaa
    16141 aaataaaaaa acatagaa
//
LOCUS       DARBFD010000058        15976 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000058 DARBFD010000000
VERSION     DARBFD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.058"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..1475
                     /locus_tag="SCK08_001833"
     CDS             138..1475
                     /locus_tag="SCK08_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEG1201706.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="SCK08_001834"
     CDS             1523..3652
                     /locus_tag="SCK08_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEG1201707.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="SCK08_001835"
     CDS             3627..4316
                     /locus_tag="SCK08_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201708.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="SCK08_001836"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="SCK08_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="SCK08_001837"
     CDS             complement(5546..5740)
                     /locus_tag="SCK08_001837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201709.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="SCK08_001838"
     CDS             complement(5790..6230)
                     /locus_tag="SCK08_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEG1201710.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="SCK08_001839"
     CDS             complement(6234..7079)
                     /locus_tag="SCK08_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEG1201711.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="SCK08_001840"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="SCK08_001840"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEG1201712.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="SCK08_001841"
     CDS             complement(8703..9650)
                     /locus_tag="SCK08_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1201713.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="SCK08_001842"
     CDS             complement(9873..10223)
                     /locus_tag="SCK08_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1201714.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="SCK08_001843"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="SCK08_001843"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEG1201715.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="SCK08_001844"
     CDS             11646..11966
                     /locus_tag="SCK08_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1201716.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="SCK08_001845"
     CDS             11988..12452
                     /locus_tag="SCK08_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1201717.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="SCK08_001846"
     CDS             12657..13262
                     /locus_tag="SCK08_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEG1201718.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="SCK08_001847"
     CDS             complement(13301..14590)
                     /locus_tag="SCK08_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEG1201719.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="SCK08_001848"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="SCK08_001848"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEG1201720.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..15976
                     /locus_tag="SCK08_001849"
                     /pseudo
     CDS             15700..15976
                     /locus_tag="SCK08_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5035 a         2942 c         3256 g         4743 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagcta
//
LOCUS       DARBFD010000059        15951 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000059 DARBFD010000000
VERSION     DARBFD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.059"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(387..809)
                     /locus_tag="SCK08_001850"
     CDS             complement(387..809)
                     /locus_tag="SCK08_001850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEG1201721.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(823..2229)
                     /gene="atpD"
                     /locus_tag="SCK08_001851"
     CDS             complement(823..2229)
                     /gene="atpD"
                     /locus_tag="SCK08_001851"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEG1201722.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2252..3154)
                     /locus_tag="SCK08_001852"
     CDS             complement(2252..3154)
                     /locus_tag="SCK08_001852"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEG1201723.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3168..4724)
                     /locus_tag="SCK08_001853"
     CDS             complement(3168..4724)
                     /locus_tag="SCK08_001853"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEG1201724.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4747..5289)
                     /locus_tag="SCK08_001854"
     CDS             complement(4747..5289)
                     /locus_tag="SCK08_001854"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEG1201725.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="SCK08_001855"
     CDS             complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="SCK08_001855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEG1201726.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="SCK08_001856"
     CDS             complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="SCK08_001856"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEG1201727.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="SCK08_001857"
     CDS             complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="SCK08_001857"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEG1201728.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="SCK08_001858"
                     /pseudo
     CDS             complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="SCK08_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10026..10781)
                     /locus_tag="SCK08_001859"
     CDS             complement(10026..10781)
                     /locus_tag="SCK08_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1201729.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10884..11120)
                     /gene="secG"
                     /locus_tag="SCK08_001860"
     CDS             complement(10884..11120)
                     /gene="secG"
                     /locus_tag="SCK08_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEG1201730.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11238..11516)
                     /locus_tag="SCK08_001861"
     CDS             complement(11238..11516)
                     /locus_tag="SCK08_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1201731.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11629..12597)
                     /locus_tag="SCK08_001862"
     CDS             complement(11629..12597)
                     /locus_tag="SCK08_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1201732.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12812..12856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12880..13854
                     /locus_tag="SCK08_001863"
     CDS             12880..13854
                     /locus_tag="SCK08_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEG1201733.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13859..14434
                     /locus_tag="SCK08_001864"
     CDS             13859..14434
                     /locus_tag="SCK08_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1201734.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14501..15640)
                     /locus_tag="SCK08_001865"
     CDS             complement(14501..15640)
                     /locus_tag="SCK08_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEG1201735.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4357 a         3443 c         2856 g         5295 t
ORIGIN      
        1 ctgagccaga atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt
       61 attgacaaag agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat
      121 catctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg
      181 tttctctaat actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga
      241 agtagcttct tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa
      301 attccttctt atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa
      361 acacggataa acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt
      421 gctcggtgca acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct
      481 tcttcgattt ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca
      541 cgttcacgtt ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt
      601 ccgccattaa cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact
      661 tcatcaatcg tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca
      721 gctgtcgtac gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt
      781 accacattta ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt
      841 cgctttttct acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc
      901 atcgtatttg ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata
      961 tgaacctgga agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg
     1021 gactctgcgg gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa
     1081 gatagcaatg atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc
     1141 tactttataa tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa
     1201 tggatctacc gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc
     1261 atccaaatgg gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata
     1321 gattgcttgg atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg
     1381 acccatttct gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa
     1441 ggctgatact tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac
     1501 atcttgacct tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg
     1561 catacgtgca cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac
     1621 tcctgaatct ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc
     1681 ggtaaataca gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag
     1741 tacggtttta ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg
     1801 ggcaagcaaa tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc
     1861 gtcaaaggct ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt
     1921 ttctaaatcg atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac
     1981 aggaacagag attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt
     2041 tgattccatc gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac
     2101 aacttttgat ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa
     2161 ggattggtct aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc
     2221 tgaactcatc ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa
     2281 tttcggtaat ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct
     2341 catcaatgat attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat
     2401 gttctgctgt tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca
     2461 ataactgaga caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag
     2521 ttgcttcttc tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag
     2581 ttagcgaatt gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt
     2641 acatagttgt tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata
     2701 gattgcgcag ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc
     2761 cgccaattgc gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca
     2821 tcgtttgttt caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga
     2881 cgatataacc cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc
     2941 ctctaggatt ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt
     3001 ctcttacttt tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact
     3061 tagaagcaga caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga
     3121 tacgtgtctt gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga
     3181 atggatttca atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct
     3241 ttatattcct gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg
     3301 attgtttcaa agatatctga atgattggta tccagatatt cgaataattc atgttcgaag
     3361 tctaaaatac tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc
     3421 actacttgtt tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta
     3481 cggcgtccac gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta
     3541 aatgcttcta attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc
     3601 attgctttga tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct
     3661 ggacgagtac ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa
     3721 ataacgtttg ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag
     3781 gcagtcattg aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt
     3841 gagtgtaagt agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc
     3901 aaggaaagtt cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca
     3961 tgtttcccgt tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa
     4021 agcaacggcg ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca
     4081 tattttttca atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca
     4141 tagatacaga tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt
     4201 ttccctgttt tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga
     4261 gcgtcgattg cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg
     4321 cctggtgcca ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca
     4381 attggctgac ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc
     4441 atgatctttc ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg
     4501 ataatgatac ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa
     4561 aattcaagca actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca
     4621 cctacataag ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt
     4681 tgttctttaa tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc
     4741 tttgtactat ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc
     4801 gattactcga tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg
     4861 ttttaattcg actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg
     4921 ttgttcgtct gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc
     4981 atcatatcga cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc
     5041 ataaacaact tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat
     5101 gatttctcgt ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg
     5161 tacttgttca tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt
     5221 tgaatcgatc gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc
     5281 tagtttcatt tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt
     5341 ctggagataa ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat
     5401 cgtcttttac agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac
     5461 tttttagacg agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc
     5521 cactttcttt cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt
     5581 gttcgcgttc ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat
     5641 cattggcgat cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat
     5701 gcttcaagag agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca
     5761 tcgcattgcc aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt
     5821 gaagtacttc tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat
     5881 actgattcaa ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa
     5941 cagcttcgac tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag
     6001 gttggcgagc cattgattcg attgttttag agataacttg accgttacca taaccggctc
     6061 cgattgctgc tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc
     6121 taaattagat aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta
     6181 tggctcatat aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac
     6241 aaggagaatg cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct
     6301 aagcttatta ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca
     6361 taaagacgta aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac
     6421 ataaaactat atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc
     6481 accccaaata agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg
     6541 tctgctgtcg ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa
     6601 ccgatgttgt ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg
     6661 ctcaattctt ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat
     6721 tctatcgcat tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct
     6781 aagaagaaaa caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa
     6841 attggtccaa tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg
     6901 aaattcacat aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac
     6961 acggtatcat accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt
     7021 ttttatttca gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga
     7081 aaaaacctat gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta
     7141 aaagcaaaag caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa
     7201 tgttcgatca atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga
     7261 ttttttccct ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt
     7321 taaaggaaca acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt
     7381 tttgtgtaat aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc
     7441 ataaggactg atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc
     7501 atcttttaga ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat
     7561 ccctgcttcc atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat
     7621 taattttccc tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt
     7681 cttcttttta acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt
     7741 actatcgcta tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt
     7801 tccttcgcgt tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc
     7861 ttctgcggaa acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt
     7921 tactcgcact ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa
     7981 atgattttca acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc
     8041 tactgtattc ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc
     8101 gaattcttcc cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg
     8161 ttctgtttct actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc
     8221 cgtagcccac gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg
     8281 atggacgatt agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc
     8341 cgctaagcca taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag
     8401 caacatagta ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg
     8461 taaatctttt ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc
     8521 aaaatcaaag aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa
     8581 cggcagattt aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga
     8641 ttcgatcaag cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc
     8701 agcatcaaca aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg
     8761 catcgtttca aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc
     8821 atagcgttgc atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt
     8881 catccgttct gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt
     8941 tggatcaatc tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca
     9001 gataccattc gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact
     9061 tgtccctctt tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac
     9121 atctgcaatg tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc
     9181 atcatctaga tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc
     9241 tctacgtcca gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa
     9301 tacatcatca ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat
     9361 gccagggtca ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata
     9421 ccctttttca gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac
     9481 tccctctgct gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac
     9541 gccgtaaaga tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa
     9601 ctgagtagtc gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc
     9661 tgtatttgct cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt
     9721 cgcttctttt gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc
     9781 tcgttcattt gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt
     9841 tcctttttta ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag
     9901 taatttaaaa tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa
     9961 actgcgtttg cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt
    10021 catatttatt cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat
    10081 tcgcgtcttg ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc
    10141 actcgctgac ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca
    10201 tctttcccag cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg
    10261 attgctgcct gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact
    10321 gctttttcat tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat
    10381 ttttcaaagt tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg
    10441 ccgccaatca ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat
    10501 cctaatacgg caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg
    10561 tctgcccacc agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt
    10621 cctttaaaaa gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt
    10681 cgaacatcgt ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt
    10741 tttgcataaa ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac
    10801 catagtttct ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc
    10861 cgttttgcga gaggcttatt caactacttc gaagataaat atgctaggac aaataataag
    10921 atcatccaga tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga
    10981 gctttttgtt tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt
    11041 tgcttgcttg gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt
    11101 cccaaaatta aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt
    11161 tatcatagat aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa
    11221 actttcagct gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca
    11281 aactcttttt ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat
    11341 tctactccag tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca
    11401 aatgtatggt caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc
    11461 tcttgttgtt catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc
    11521 cgcactcctc aaataactga tattttttta ttcctacctt attttagcag tctcctgatc
    11581 tataaacaat tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt
    11641 tattctttgt tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga
    11701 tgcttccaag tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt
    11761 ttgaatcgtg tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa
    11821 taaaggcaga tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc
    11881 ggcgtatttc tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt
    11941 gattttactt aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat
    12001 aaatcctttg accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc
    12061 cattgatccg ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat
    12121 ccaggcaatt aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc
    12181 tccgtgcgca cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc
    12241 tatatgagac atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt
    12301 attctgctta tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt
    12361 gattgttttg ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg
    12421 ttttttttct actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata
    12481 ccagtcatat cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag
    12541 aaaatggtaa ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt
    12601 acacccgccg ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg
    12661 attctttatt ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag
    12721 ttctcctcaa tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac
    12781 taatttcgtg cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat
    12841 aaaaaactaa gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat
    12901 tgcgatccag ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc
    12961 gaagtagttg ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat
    13021 gtgctgaaat gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa
    13081 aaacctttga agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg
    13141 gaaacaaatt tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt
    13201 ttggctgatt atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc
    13261 aatatcgctt ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc
    13321 tcgatgggtg gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac
    13381 tggtgtgatc ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg
    13441 gttgggttag atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt
    13501 caaatgaatc ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc
    13561 cactggaaac aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat
    13621 tttatcgagg gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga
    13681 ataagtcgtg gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt
    13741 aaaattatga caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta
    13801 aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat
    13861 gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa
    13921 ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg
    13981 aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag
    14041 cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat
    14101 tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag
    14161 cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat
    14221 cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct
    14281 attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga
    14341 ctaccttgat cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca
    14401 acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata
    14461 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
    14521 taattccttg atatttttca atcatatcca aataattatc gatttcaaca tactcatcta
    14581 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
    14641 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
    14701 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
    14761 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
    14821 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
    14881 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
    14941 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
    15001 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
    15061 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
    15121 gcgtatacat caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta
    15181 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
    15241 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
    15301 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
    15361 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
    15421 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
    15481 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
    15541 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
    15601 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
    15661 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
    15721 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
    15781 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
    15841 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
    15901 acatcatata cagaagctag ttgcaaaaaa aactctctac ctgctgatat t
//
LOCUS       DARBFD010000060        15759 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000060 DARBFD010000000
VERSION     DARBFD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.060"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            119..1546
                     /locus_tag="SCK08_001866"
     CDS             119..1546
                     /locus_tag="SCK08_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201736.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1638..2003)
                     /locus_tag="SCK08_001867"
     CDS             complement(1638..2003)
                     /locus_tag="SCK08_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1201737.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(2000..2365)
                     /locus_tag="SCK08_001868"
     CDS             complement(2000..2365)
                     /locus_tag="SCK08_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1201738.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2557..2805)
                     /locus_tag="SCK08_001869"
     CDS             complement(2557..2805)
                     /locus_tag="SCK08_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201739.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2804..2932
                     /locus_tag="SCK08_001870"
     CDS             2804..2932
                     /locus_tag="SCK08_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEG1201740.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2989..4683
                     /locus_tag="SCK08_001871"
     CDS             2989..4683
                     /locus_tag="SCK08_001871"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEG1201741.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4687..4806
                     /locus_tag="SCK08_001872"
                     /pseudo
     CDS             4687..4806
                     /locus_tag="SCK08_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4783..4956
                     /locus_tag="SCK08_001873"
     CDS             4783..4956
                     /locus_tag="SCK08_001873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201742.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5334..6467
                     /locus_tag="SCK08_001874"
     CDS             5334..6467
                     /locus_tag="SCK08_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEG1201743.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6498..6818
                     /locus_tag="SCK08_001875"
     CDS             6498..6818
                     /locus_tag="SCK08_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEG1201744.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6847..7311)
                     /locus_tag="SCK08_001876"
     CDS             complement(6847..7311)
                     /locus_tag="SCK08_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1201745.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7443..8696)
                     /locus_tag="SCK08_001877"
     CDS             complement(7443..8696)
                     /locus_tag="SCK08_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1201746.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8725..9042)
                     /locus_tag="SCK08_001878"
     CDS             complement(8725..9042)
                     /locus_tag="SCK08_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1201747.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9068..9388)
                     /locus_tag="SCK08_001879"
     CDS             complement(9068..9388)
                     /locus_tag="SCK08_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1201748.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9852..11267)
                     /locus_tag="SCK08_001880"
     CDS             complement(9852..11267)
                     /locus_tag="SCK08_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1201749.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11284..12600)
                     /locus_tag="SCK08_001881"
     CDS             complement(11284..12600)
                     /locus_tag="SCK08_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1201750.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12750..13448)
                     /locus_tag="SCK08_001882"
     CDS             complement(12750..13448)
                     /locus_tag="SCK08_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEG1201751.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13737..14693)
                     /locus_tag="SCK08_001883"
     CDS             complement(13737..14693)
                     /locus_tag="SCK08_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1201752.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14806..15639)
                     /gene="msrA"
                     /locus_tag="SCK08_001884"
     CDS             complement(14806..15639)
                     /gene="msrA"
                     /locus_tag="SCK08_001884"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1201753.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4905 a         3191 c         2696 g         4967 t
ORIGIN      
        1 aaaacctttt ttaacggcat ttatatgctt tttcagaaat taactgatga tttcaaaaag
       61 aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag gctctactat
      121 gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg ggcaattcta
      181 tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac
      241 tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg cttatacaca
      301 tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat gcaagatcat
      361 gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt tggaagaaga
      421 tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca aagcttattc
      481 attaaaaaaa caagtattag cttactttaa tgactgcggg atcgaaatcg accgctactc
      541 ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg
      601 cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc gtttcattga
      661 atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa tcttatcgat
      721 tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag attcaaaatt
      781 tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat gggaaaacac
      841 tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac tattcaatct
      901 ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac agctacacca
      961 agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg aatctcactt
     1021 caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc
     1081 ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa atgaggaaca
     1141 gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc agttacctta
     1201 tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga gcggaattct
     1261 ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg ttcattatct
     1321 gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg caccaacaat
     1381 ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc gggtattatg
     1441 tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc ctatttcgat
     1501 caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa
     1561 aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat ctcttgatac
     1621 acgtcaggct cacaacctca cttattttgg acatctaggg gtccgatctc tttatttaaa
     1681 aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat atcctgcaag
     1741 gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa acttagaagt
     1801 atatagcctc ccaacatctt tttaatagca ttgttctcgc ttactcggtc gctttgcact
     1861 tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac accccaaacc
     1921 aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata tggaagaaga
     1981 aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct ttgtcttcta
     2041 tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt gcaagcttca
     2101 aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg actagtactt
     2161 ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta cgagtattac
     2221 gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga acaagaacga
     2281 tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc cattctagaa
     2341 attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt tgttcctatt
     2401 cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct aatgataacg
     2461 gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca taaatcgtgg
     2521 aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca gcttttcaaa
     2581 gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac catacacctc
     2641 ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag acacttcttc
     2701 ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta ataaaaacga
     2761 actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat
     2821 caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag gaaaaaagac
     2881 aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct agcatcctat
     2941 ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat gagaaaaagt
     3001 aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta tttgattcaa
     3061 gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca cggttcaaat
     3121 gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca tccattagaa
     3181 aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac ctatgtagat
     3241 ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac tgctgaagaa
     3301 gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt gatggaaggt
     3361 ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg tattcttctg
     3421 actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat cactatcgaa
     3481 gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat gtgggaaaca
     3541 acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat gcatcaaatg
     3601 atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa
     3661 tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg caaaatcatt
     3721 ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga tgaaatcact
     3781 gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt tgtggtcgat
     3841 gaagatacag cagttatttt cactaatggg tccattacag attcagctac tttaggagat
     3901 tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct ctggaaaaaa
     3961 gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga tcgagatgca
     4021 agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc
     4081 cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac cacaccgatc
     4141 acgccgttag gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca accgcatttt
     4201 acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc aagacgacgt
     4261 ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct agaagaactg
     4321 attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa
     4381 attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc cgcgttgttc
     4441 aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac caatctggcc
     4501 tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca aagtgctgta
     4561 cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa
     4621 acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa
     4681 taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac caggctaaaa
     4741 cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc ttttgaacac
     4801 cgtttaccaa gaggttatga aaaagctgcc ctgcatcaag taataagagc agccaagcta
     4861 aaacagcttc ttatgttttt gacttggtta gacttattac cgcagatgca agcttttgaa
     4921 caccgtttac caagaggtta tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg
     4981 tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa taccgagaaa ctgatccctt
     5041 ctgtcataac ctctttttat ttatgtctgt ttctttttga aaacaactat tttttcaacg
     5101 tttactctct tgccaatcca acatcatcta acggatacaa ctatttcaat gattaccatt
     5161 acactaaaaa atcggaaaca cacctctccg aacacaaata cataagacta tagaatgttt
     5221 catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa aatgaaatta
     5281 tctgtagtac taataatgca gacaatttta tgaatctgaa aggagcatct ccaatgaaag
     5341 cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat
     5401 tagaaccaac agatgcaatc atccgtatca cagcaacagc catttgcggc tccgatctgc
     5461 atctttacca tcatggtgaa gcagttttgg aaaaagggta tgttgtagga cacgaaccaa
     5521 tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg
     5581 ttatcccttt taatattagt tgtggtcatt gtcatttctg cctgaatcac atggaaagcc
     5641 aatgtgatga atcaaatcca gatcagttat atggcgggct tttcggattt ggcaagttga
     5701 acggtaatca ttggggtggt caagcggaat atctaagagt tccttatgca gactctactt
     5761 catttgttgt accagataat gatcttcctg atgaaaaagt tttatttcta tcagatattt
     5821 tgcccacagc ttattggagc gttgaaaacg caggagtaaa aaaaggcgat acagttgtga
     5881 ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg gctggagcag
     5941 agcgcgtaat cgcagtagat ccagtacagc accgtctaga taaagcggct aattacaatg
     6001 gggttgaaac ttatttctta gaagacacgg cacaagctgg aacagaattg tatgaactgg
     6061 ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat ggacggatta gagcctgtca
     6121 aagaaaaagc gaaaaattta gtaagcttac aaagcggcac aatctctcca ttacagatgg
     6181 catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat atgactccag
     6241 cttcttctta cccgctgcaa gaatttttta tgcgggatat cgctgtcaag catggacagg
     6301 caccaattat ccatctaatg ccaaaaattt acgcaatgat tgcagaagga ctattcgatc
     6361 ctacacaaat cattactcat tcgctgccac tatcagaagc agcacaagca tatgagatct
     6421 ttgacaaaaa agaagataaa aatataaaag ttgttttgaa accttgaatt cagtaaaaaa
     6481 tacaggtgat tttctttatg cccaaaatca aacgaaatac ccatgagatc gaaataggaa
     6541 aagaagaaga tatcgaaata cgaggacgtc gcttccgctt ctatttccaa aatcgttaca
     6601 cattgatctc actggctgtc gatttattaa ccgggatttt ctatattttt ggaagccttg
     6661 cttccttaac taaaatcccc gatcgatatg gcacctattt ttatttggct ggtgctattt
     6721 tcttgacgat tcgtcctatt ctcagaatcg taaggaacat ttttatttac gacgaaaaaa
     6781 aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct
     6841 cttttttcac cctatcgtat cactttgata cagacgagcg gtcgtcccac tttcttcctt
     6901 gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat ctgtaattaa
     6961 gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg atttccgcta atatccgctt
     7021 ggctatcccc tgcttacgct tttccggttc aacaaacagg gcacaaagat ttggtgtcag
     7081 atcagggcgg ttatggaagt cgttttcaat gacgcccgct cctccgacga tggtttcttt
     7141 ttcatctaag accacatacc attgaggaat actttcctta ttagcgattc cagtacgaat
     7201 actttcctca tacacaacag caggaactcc ccattttgat gaaaaccaat tcgcgctttg
     7261 ttttactaat tctgggtgtt ctcttaattt gatccagtta tatgtaacca atgtttaaac
     7321 ctccgactaa aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga
     7381 caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac ttgttctccg ttttttgatt
     7441 aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt gacgaacggt
     7501 agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc tgcaatactt
     7561 tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat aacggtagct
     7621 gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac cattggtgtc
     7681 aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa catcgttggt
     7741 tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat atatggtttg
     7801 tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc tgttcctcct
     7861 aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct atgtccagct
     7921 gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat caaaggatcg
     7981 accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa tgcaagcagt
     8041 aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac tgtatagaaa
     8101 gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa agcaaacgca
     8161 gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca
     8221 ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa gatttctgtt
     8281 gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc taatgctccg
     8341 cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc ttttcggtag
     8401 ccgttagcta aatgataagc aagcaagaaa gcaatcaaga cagaaagaat ggcaaatgta
     8461 ccgttccaag cagcagcccc gaatcctttc catgattcac caccaaaaat tgagttcatc
     8521 aattcttgga acccgggaat cggtaagtta ttgatcatta ctgctaatgc tcctaagatc
     8581 attaacggca tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg ttgtgcacca
     8641 atttttgatg ctacaggtat aaaatgtttc tccataaatg caacaaattt atccataaat
     8701 cttccctctt ttctttactg tagattacgc gttcaatcga cgatacagat taatgatctc
     8761 ttttgctaaa tctgtaaaag ctatagatgt catcagatga tcttgactgt ggaccgtcaa
     8821 aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg tttgagagtg
     8881 atgtgcttgg actaaagatt cctctgcatc tttgattttt tcatctgcta aagtgaaatc
     8941 accttttttc gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc cgccaaacat
     9001 gatcagaccc atgatggctt ctagattttt ttgatcttcc atttattgta tcctccgata
     9061 atttttatta tttgtttatt agtttttctg cttggtcgag tactttttca ccattcatca
     9121 tgccatagtc agccatattg attacatcta aaggaatgcc tttaggtgct agcttttcag
     9181 caaattgatt ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct acatttttag
     9241 attctagttg attatccgca tctgaagcag atacagcaaa gatgtctgct tccattcctt
     9301 ttgcttctgc tgccttttgc atttttgtga ctaggagact tgtactcatt ccagctgaac
     9361 atacaagcat gattgttttc ttttccattt taaacacgtt cctttctata cttacacttg
     9421 tatataagca agtagcgtgc caactttaaa agcgctttaa aaagttgata tcatcacatt
     9481 taaggtctat accaagttgc atacacacta aaaaagaaag cgcacactaa acacacaaat
     9541 taatttttat tacctttttt cttttaacgt gcacaaaaaa agttgtgaca caaaacttgt
     9601 cacaactttt ttcaaataaa cggtattcaa aagcttgcat ctgcggtaat aagtccaacc
     9661 aagttaaaaa cataagatgt tgtttttact ttgctattct tattacttga tgcaggacag
     9721 ctttttcaca acctctcaat aaacggtgtt caatcagctg accctgggta ttagctcaaa
     9781 acgctggatt cacaacctct tttatttcaa ataaccagta aatacaatga acatctacaa
     9841 aaaacttcta tttatttata gatttcgtca ggcaaatcca attctctctt atcaatcaaa
     9901 gtctgatacc acgaagcaga ttttttcaat gttctgcttc gttcatcgtt caattcgatt
     9961 cgaactaatc catatcgatt cttaaatgca ttcattggtg acacacagtc agtgaatgcc
    10021 cataacatat agccttcgca atttgcgcct tcttctgttg ctttgatcaa gtaatgcaaa
    10081 tgctgtccga taaattcgat acggtaatca tcttgcacga ttcctttctc atccatatac
    10141 tgttcttcat attctcttcc cataccgttt tctgttatca gccaagggat attatgataa
    10201 ttttctttta gatacatggc tatatcatac atgattttcg gatagatttc ccatcccctg
    10261 gaattattca ttttcttccc aggtaaatca aagacttcat aatatcgttc aggatggaaa
    10321 ggtgtctctt cattccattg ataacgaggg gcgcgaacgc gtttaggata atattgattg
    10381 attccaagga aatctactgt atgatgctga atcgtttcta aatcctctgt tgcaggatcg
    10441 aaataaatcg attgtttttg acacaactcc aggaactctt ctgtgtagta tccttttacc
    10501 atcggatcaa aaaatactcg attataaaac aaatcataca tataagcagc tttttggtct
    10561 tctggagaat tggatcgagc atagaccatt tcaggattta aaatacagcc aatccttcca
    10621 ccataatttc cgctgcggta aatctctact gctttagccg ttgctaaaac tttatgataa
    10681 ttccacatca tccacttttt tgtgttttgt tcatgcggcc agcgaacagc atccaaatag
    10741 caacgcgttt ggggaacgat tggttcgttg aatgtaaacc agttttttac cctatcaccg
    10801 taacgatcaa aggcaatttg agcatactta gcataaaaat caactacttt tcttgaagac
    10861 cagccatcat atttttccaa aagaaaagct gggacttcat aatgttccaa acaaatcatc
    10921 ggttctacgt ctgcttcgat caaagagtcg atcatttcac ttatatgctg agcatattct
    10981 tcatctacta tacagttctc ataatcagta aaaaaacgag cccaattgat agaagtccga
    11041 taatgtgtca gatggatgga tttcatcaga tccacatcct gctggtattg ttccataaaa
    11101 ttagttgcta cggctgggcc atacccatta tgccaaacaa aatgttcatt tttataccag
    11161 ctatctataa acgaatcctg tccttctttt tttcctttcc aaccttccgt ttgccaagca
    11221 gaagatgcag caccaagaat aaaatttttt ggaatagtaa ttttcatgat ttcctcctgt
    11281 tttttagatc ccatcatttt ccacacgatt tgctactttt acaaatggca gatagatcaa
    11341 aatagaaaca ataatacaaa caagttgcgt aataactgca ccgatatttc ctgctgttgc
    11401 cagataagca ttgacgatcg gcggcattgg ccaagggacc attacaactg ctttcgccgc
    11461 aaatcctaaa gcggttgcaa aataaccaat cgttccagtg ataagaggcg ttaagataaa
    11521 cggaatagct aaaattggat tcagcatgat cggcatacca aaaataactg gttcattgat
    11581 gttgaagatc ccaggagcaa gggataactt ggtgatttgt ttcatatctt cccgttttcc
    11641 aactaacaat actgcaatca gtaaaccgat cgtgactcca gacccgccca tactcatata
    11701 catatcccaa aatggcatag taataatgtt cggtacttct tttccagctt caaaagcttc
    11761 cgtatttaca gcaattgccg ctaaaagcaa cggttcccgt atcggcttca ccatttgatt
    11821 tccatggata ccaatcaccc aaaagacttg cgcaataaac atcaatagca aaatacccgg
    11881 taacccttgt acgacacctt ctaatgggcg ttgtacgaca ttgtaaatga tatcataaag
    11941 gtacattcct gtaagttctt tgatgataaa tcccgcagcc gcgatacccg atacagtcag
    12001 aatcgtcggg aataaagcag aaaaactagc agaaacattc gttggcacgg tatcaggcat
    12061 tctcagctgt agtttcttct gtttcctcaa ccagcaataa agttcaatag aaaggatgga
    12121 gaccaacatt cccaaaaata accctttcgt atctgtatat tgtttagcca atacgttttc
    12181 tactataaca gtctgatttt cattcactaa ttcataaaca gttggattaa cagataaata
    12241 agacatcact gcgatgatac cagggaatac accatccact ttattcagct tggctaattc
    12301 gatccctatc agaaaaacag catagatggt caagaaactt agtgtgacat agctgatggc
    12361 agatgcgata ggctttaatt cttctaaaaa acgcaagaaa cttatctgtg ctaagccatt
    12421 ttccgagtca aagaccattg ctgaaaacaa tgtgccaaaa gcacccgtaa taattaccgg
    12481 aatcaatgca gcaaatgcgt tttttatcac catgatatac cttaaattat tgattctcgt
    12541 tgccacgttc cctaaaacat tggctaattt atccataaaa ctcattccag atcccctcat
    12601 tcctgcttct tctccttgac gtcatctctt tttgtctatt agtcagcgct tacatttcga
    12661 tgaacagaaa ctatttactc ctctttttta ccaaatataa aacaaaaatt catctgatgt
    12721 atttatcata aaatacatca gatgaactgt caattagttt ctttttctcc tattattttc
    12781 tgtaattctc tccgatttat tgccatatgg acagtcattg catcaaacgc agcaagtgca
    12841 ttccgccttt cgatggcctg aacgatctct gtatgaatat cgatcgtctc gattttcaga
    12901 tgctggatat cataattttt attaaacaaa gcaattgatt cattaatgat tgggataata
    12961 tgcagcaagg ctacgtttcc gctagcttct gcaatcatcg tatgaaaatc gatgtctaat
    13021 tgaacatgtt cttgtgtgcc ttctcgaaaa gaagtttcaa tcttttgcgt taattttttc
    13081 atttggacga tttgattctt cgttgcatat tccgctgcca aacctgccat cttaggctcc
    13141 aataaatatc tcagttcaaa taagtctcgt atcatccgat cctgatcttc aatcaaagaa
    13201 aaaccgagtg ggtcattggc aatccctcgc ttatcactga caaatgtccc cgctccttgc
    13261 cgaacttcaa ggatattcct ggaagctaga gcccgcacag cttctcgcac cgtgcttcgc
    13321 cccacctcta aataatcgga caattcatat tcattcggca gtttatctcc tacttgcaac
    13381 tgcatttcct ttaaatatct aaggatcttg tcagtagtta cttctactag ggttttattt
    13441 tcttccaaac cacatacctc cttttcatct gatgtatttt ttcgtaatta ttataacacg
    13501 tttgaatcat ctgatgattt tttgtataca aaattgatta atccgttcat agacatacaa
    13561 acgaagaaga tatttaccat aaaaaagact aggataaaat tcactcctag ccttcgatca
    13621 tttttatttt gaacgacaaa aaatatgatg ttcaataaca gacgatcttc attcgattct
    13681 tgatgtcatc ttttgttttt tttaatatat ggataaaaag tttgattaca tcgtttctat
    13741 tctagaattt ggtcgattct caattcttct tctggtgaga aatctaaatt atccatcatt
    13801 cgaacacttt cttgtaactg acttaaccga cttgctccga ccagtacact tgtaacagat
    13861 ttttgtcgca aattccatgc aagggccatt tgcgctaatg attgtcctcg tgatacggcg
    13921 attgtttgca gtgctttgat tttttccaga gttgatccaa cttgttcttc tgaaaggaat
    13981 ggtatttcct tacgatgtgc tctagaatct tcaggaatac catgtagata acgatttgtc
    14041 agcaaccctt gagcaagcgg actgaacgtg attgccccta gcccttcttc ttctaatact
    14101 ggagatagat cgtcttctag tagtgcacga gaaaacatat tgtagcgcat ctgatgaata
    14161 atgaacggtg cttcctttct ttttaaaatt tcagtcattt tttttgtatc ttctccatta
    14221 tagttggata taccaatata taatgctttt ccttgtctta caagttgcat caaagcttct
    14281 gctgtttctt ccaaaggtgt atcagggtct gggcgatgat gatagaagat gtcaacataa
    14341 tctagcccca ttctttggag actttggtca caacttgaaa tgatggattt acgagagccc
    14401 cattccccat atggcccttc ccacatatga taacctgctt tgctagaaat aatcagttca
    14461 tcacggtagg cttgaaaatc ttctctaaaa atacgcccaa agtttttctc tgcagctcca
    14521 gcagggcctc cataattatt cgcaaggtcg aaatgggtaa ttcccatgtc gaatgcgcct
    14581 cttaaaatct ctctttgatt atggatagga tcataatcac caaaattttg ccataatccg
    14641 agagataaaa tcggtaattt caaacctgaa tgaccggatt tacgatattc cattttttca
    14701 taacgtgcac tatctgcaat atacaatttt tctcctcctc atcaaatctt tttaaaacgc
    14761 tttctttttt ctatttttag tttactacat tttaaagaaa aggagttaat caaaatgatt
    14821 ttttcgtata tttttttgcc atttgttttt tactcgttga tagatcagaa attgattacg
    14881 tgcttgctgg atttttttat aacgtttcgg ttgttttttg taaaattgct gatgatagtt
    14941 ttctgctggc caaaacgtac cggctggttc gatttttgtg acgattggtt ggtggaaagt
    15001 tcctgaactg tctagtttct gttttgattg ttccgctaat tctttttgtc gttcgacttg
    15061 ataaaaaatc actggacgat attgcttccc tctatcttga aattgtccaa atgtatctgt
    15121 gggatcagta atttgccaat agattgtcag caactcgcta taacttattt ctcttgtgtc
    15181 ataaatgatc tccacagctt ccacatgacc tgtgtaacca ccacttactt gatcataatt
    15241 tggtttttca acatgtccgc ccgtgtatcc tgacaaaaca gataggatcc cttttttcga
    15301 ttcaaacggc tcgaccatac accagaaaca tccccctgca aatactgcac gttcttgata
    15361 gtcagcatct tgcatggcat gcttggaaaa gtcatatttt tgctccttat caaatccttc
    15421 cgtaatcaat aaataaaagt ccattacatc aggcgtcaga ttcattcgga tagctaatgg
    15481 acgtagtgtt gcttctagtt ttgataactg ttctttaacg gatatatttt ctcttatgtc
    15541 attctttgtc tgaatcaata cttttcgttc ccaatctcgt gtattcggat tcaaaatcag
    15601 attataaagt gtttgaatga atatttgttc ctcatccata gattctcctc ctttccgaag
    15661 aagacaagct agtgacttta gctgtaattt atcataaagt gaggaaataa gcgattactt
    15721 catcccccta cagatttttc gtataaaaaa gagagaatc
//
LOCUS       DARBFD010000061        15399 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000061 DARBFD010000000
VERSION     DARBFD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.061"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            88..438
                     /locus_tag="SCK08_001885"
     CDS             88..438
                     /locus_tag="SCK08_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEG1201754.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            537..1067
                     /locus_tag="SCK08_001886"
     CDS             537..1067
                     /locus_tag="SCK08_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1201755.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1221..2372
                     /locus_tag="SCK08_001887"
     CDS             1221..2372
                     /locus_tag="SCK08_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG1201756.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2481..4520)
                     /locus_tag="SCK08_001888"
     CDS             complement(2481..4520)
                     /locus_tag="SCK08_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEG1201757.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4578..5135)
                     /locus_tag="SCK08_001889"
                     /pseudo
     CDS             complement(4578..5135)
                     /locus_tag="SCK08_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(5674..5880)
                     /locus_tag="SCK08_001890"
     CDS             complement(5674..5880)
                     /locus_tag="SCK08_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201758.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5896..6510)
                     /locus_tag="SCK08_001891"
     CDS             complement(5896..6510)
                     /locus_tag="SCK08_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEG1201759.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6657..7340)
                     /locus_tag="SCK08_001892"
     CDS             complement(6657..7340)
                     /locus_tag="SCK08_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1201760.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7449..7907)
                     /locus_tag="SCK08_001893"
     CDS             complement(7449..7907)
                     /locus_tag="SCK08_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEG1201761.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(7991..9754)
                     /locus_tag="SCK08_001894"
     CDS             complement(7991..9754)
                     /locus_tag="SCK08_001894"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEG1201762.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9975..11435)
                     /locus_tag="SCK08_001895"
     CDS             complement(9975..11435)
                     /locus_tag="SCK08_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201763.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11643..12569)
                     /gene="glsA"
                     /locus_tag="SCK08_001896"
     CDS             complement(11643..12569)
                     /gene="glsA"
                     /locus_tag="SCK08_001896"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEG1201764.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12592..14019)
                     /locus_tag="SCK08_001897"
     CDS             complement(12592..14019)
                     /locus_tag="SCK08_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1201765.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14050..15264)
                     /locus_tag="SCK08_001898"
     CDS             complement(14050..15264)
                     /locus_tag="SCK08_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEG1201766.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4396 a         3082 c         2654 g         5267 t
ORIGIN      
        1 ttgttttttt gaataatcgc tttttcttct ctttttctta tatactataa gtagtaaata
       61 atttgaaaaa aagaaaggtg actgattatg gcgcaaaatc gtatttttac gacaagtttt
      121 gcaagcgttt atcctatgta tgtagcaaaa gcggagagaa aagatcgtat aaaagaggaa
      181 gtagatgaaa tcatttgttg gctgacgggt tattcccaaa cagaactaga atatcaacta
      241 caaaaacaag tgactttcga acaatttttt gcggaagccc ctgtccccaa tccttcacgt
      301 tccttgatta caggtgttgt ttgtggtgta cgagtagaag acatcgagga taacttgatg
      361 aaagaaattc gttatttaga taaaatgatc gatgagctag caaaaggcaa aaaaatggaa
      421 aagatcctca gaagttgatt tttccagaga tattcttctc cttcgctcat tttgtaacat
      481 ttaatttggt aaaatgacta gttcacgtta aaataagtca aaggaggttt tcttatatgt
      541 ttgagacgat taaacaaaat attcaaaaat acgccttatt aagagctgct gtttatatta
      601 tttctgggat tgcgattgtg ctaaaccctt ctgctgtttt tcattttatc ggttatctga
      661 tcaccgccta tcttgttttg cttgggttgt tgaatctttt tgaggcttac cgttttaaaa
      721 ggaaaacagg ttcttggagt atcggtctgt tcagtggtct ttttttcctg atcttagctc
      781 tgatcgtcta tgcttttgca cctgcaatcg tttctatcct gccgattctt cttggattag
      841 ctgttatcat caacagcttg ttccagttgt tcttcagcat gaatacaaaa gctaaaagtt
      901 ggagtgttta tagtagtatt ctgttgttga ttggcggatt cgtcttattg ttcaatccat
      961 tcaaaagttt gatgcttcta ttccaaatat tcggctttat tttgattttc atgggcatcc
     1021 aggagattat caatttcatc agaataagaa aaatgactat acgttaatca tttttagtta
     1081 ttaaaataga ccgtataaaa accaacaatc caaaaaaatc ttttggattg ttggtttttt
     1141 tgtgagtata ttttcctatt ttttcaataa tgtatgttat gctaagttcg ggctatattt
     1201 tatacaaagg aatgatgaga atggaattta ttggtatttt atgcctgata ttgatttcaa
     1261 caacgattgg ctcacatatc tcgcggagat taggtatccc agcagtgatt ggacaattac
     1321 ttgttggtgt tttgctaggc caagctggat tgaattgggt caaacctgat attctagtcc
     1381 atgacttttc ggagattggt gtgatcttat taatgttttt agctggtctg gaaagtgatt
     1441 tatctctttt gaaaaaatat ttccgacctg gtatgttcgt tgctttgctt gggatcatct
     1501 tcccggttgt ttttggctgg ttgacaggca aagcattcca agtttcggga aatgaagcga
     1561 ttttctttgg gattattctt gcggcaactt ctgtcagtat ttcagtagaa gtattaaaag
     1621 aactaaacgt agtgaataca aaagaaggat caacaatttt gggtgcttct gtggtagatg
     1681 acattttagt cgttttagtt ctaagtttca gcctatcttt tctaacgggg gaagcttcta
     1741 gcaatgcttc tttaccgctt gttttgattg aacagctaat ttattttgtt tttattttct
     1801 tattagtcaa atggattgcg cctttcctac tttcatttgc agaaaaagtg tacgcaaatt
     1861 cttctattat catcgtatcg ctagttattt gtttaggaat gtcttacgta gcggatcttg
     1921 ttgggcttag ctctgtcatt ggtgcttttt ttgctgggat agctgtcagt cagacaaagg
     1981 ttaaagaaga agtataccac aatgtagagg ctttaggata tgcagtattt ataccagtat
     2041 tttttgtcag tgtaggatta gaagttgatt tttctgactt tagtgaacaa ctactcttta
     2101 tttctatatt gacgcttgta gcgattttga ccaaattgct tggaggttat tttggtgctc
     2161 agattgccgg tttttctcaa catagttcat tgatggtcgg tgctggtatg atttcgcgtg
     2221 gagaaatggc tttgatcatt ttacaaattg gacagcaaag tcaattgatc gaaccacagt
     2281 actattcacc tttagttatc gtgattttat taagtacact tatttctcca cttgttctca
     2341 aatattttac aaaaaaagtt tatgaagtgt gagtaaatca aaaagaagtt gtgaaagaga
     2401 catcgtgaaa accgtttatt tgaaaaaagg actgtgacga taacgccaca gtccttttac
     2461 tgtacttaaa aagcaatttt ttattgataa ttttggttga ggtattgcaa tagttccgat
     2521 gctcgtttgg ttgctgaatc atcatcttct ttattttcca acatgctgac catcatataa
     2581 ccttggttat caagattgaa agcaaacaag aaactgttct cttgcccctt tacatcctgt
     2641 ttttctttga tttccgctgt accagttttc gctgccaacg gaatccctaa agcagaaaga
     2701 gaatgtgctg taccatttac atcttgcaca acttctctca gatctggcac gatcgtttgt
     2761 actgctgttt cgctgatgac attcttctta tcttttgtct ctttatctgc aatcaattta
     2821 ggatagacaa gtgtgccatt gttggtaaaa acagaataca ttgctgcttg ctggatagga
     2881 gtaattagaa gttcgccctg tccatatcca gtatcagcta gcaagatatc tgagttaaag
     2941 ctatcttcat tagaaatttg tgctggattc atactaattg gcaaatcaag gtcttcacca
     3001 aaaatgaatt tatctaagcc tgtacgaaat tttttctcac ccattttcaa cgtttcttgt
     3061 gccgcatata tattatcgga atagatcaaa gcagttttta agtctacttg tgatacatca
     3121 ctactgacac gcgttgtttt aaaagatccc caagaactgt ctttttgcca tttaagcccg
     3181 ttgatcgtca acacttcatt tggatcgata gtgccgttgt cgagaccgat tgctgctgta
     3241 atcattttaa acgtagatcc aggagcataa cctgtcgcaa atcggctgat gaatggatgt
     3301 tcaggatttt cttcataagc tttgtaatct tcttgtgaga tcccgtttgt cattttgttt
     3361 ggatcatagc ttggagagct agcaagcgca agaagatcac cggtttttgg cgttgtcgca
     3421 acagttgatg cagcttttcc tcctagactg tcaaaagcta ttttttgtgc cttcgcatcg
     3481 attgtcaatt tgatatcttt gccattttga acttcatgtt cgatcagaac ttttttctct
     3541 acaccgtctg catctgtaat gcttaatttc ccacctgttg tcccacgaag atccttatca
     3601 aaagccatct ccaaacctga gcgaccgatt tttccattac tgcttaattc tggattctta
     3661 tcaatatctt ctgctgtgat atctcccacg taaccaatca gctgagcagc tgcttcaccc
     3721 aaaggataat atctgccgtc tacttcttga atggtagctc cagctggaag ttctggcgtt
     3781 gctccatcaa tgattttcaa tgggacaaag taatcgggtt gtacccagct ttgtgaaatc
     3841 gcctgattga tagcatcttc tgttaagtcg aaggaggaag caatcgcttt gatattggct
     3901 gttttttcgc ctccatctcc aagtttactt ggaacgactc ctaattgttt tagtttgcct
     3961 gttgttgcta atggttcacc atttcgatct ataatattcc ctcttgctgc ttcttgtgtg
     4021 gtcaggctta ctttgtcatt tccttccatt tctggaaaaa ccaagttggg ttgccagttg
     4081 atcgtggttt gaccatcatt tctgtctaat gttcctttat aagaaagatc tttcaattca
     4141 cctaatgagg tattcatctt tgctttatat gaaaaactgt aagtagaatc gtctttttta
     4201 tctactttta gattcgatat ctgaagtcct ttgacatcag cagcaccgta tatattctgg
     4261 tatttatcta agatctcttt ttcagataat gcacttttat ttgccgcctt ttttgatgtc
     4321 atttctgcag ctttgttata atctcccttg ttcaaagctt ggacaaattg ctcaactgtc
     4381 ttttctccag cttctactgc ttgggtttct tgatagtgct ggtagtaaaa ataaccgcca
     4441 cccgcagctg ctattaagat cactgcgccg gcaatataag cgcctgttcg atttttgccg
     4501 tgcttgtcac ttcttttcat tttttcctcc ttctcttgct tgtcattcct ttatttcact
     4561 atttatacct gtttattcta acatatgtct ttgaggaaat ccataccaca atccagaagc
     4621 acactttctg gaaggttcgt ttggatacta ccaagtaatt tcccaaccgc tttgggtagc
     4681 ttgaatattg aagttacgtc tttcagctgc tgagagatag ctttgatcac ttgctgctgc
     4741 cttctgatcc tcccgaagtc tccttctttg tcttcccgaa atcgagcata ctggagtaaa
     4801 gtattaccat ccatcacttg ctctcctttg tttatatata cgccatctaa atctagagat
     4861 ttttctgcat cgatcttgac tccaccagga aacagttcat taacgcaatc gataaaatct
     4921 tgaaatgtga tacttgcata atagggagct tcaaatttga aattttcctt taatgtttga
     4981 tttaataaat caactccccc ataagagtaa gctgcattta tcttattcat tccgtaacct
     5041 ggaatatcga catatgaatc tctcatgata gagatcagtt ttgcttgttg tgttttctgg
     5101 ttgtattgtg caaccagaag tgaatccgag cgacctccac caatcgatct tttttcaccg
     5161 catctcctcc tcacacagca atccacttgt caggtgagga gcgagtttgt ctcatcaaat
     5221 aagtcagtca caaaaatata aagaaaaggt cagaaacctt ttcgaacaag aattctgacc
     5281 tagcatcatc tcatcgtccg agttttagca ctatacaacg taaagaaaac attcttagct
     5341 attggaaaaa gccttaatcc aacagtttct gttttaccca ttggcgactt tcgacgtttc
     5401 tgggcaacag cctatgtttg tataggagcc tcacctaaca aggttattac tatattctat
     5461 ttacgagttc atgatagact tttcctaatg attaggcaat ggaatttgaa atgaaatagt
     5521 gaaatttaac aatctttaat attttttaga aaaaaataac aaataagttg aaagaatccc
     5581 gttgcaaatt ctttcagttg caccaggatt ctttgataat tttttagttt tatgtgtttt
     5641 tttatatgtt ttttcacttt tcggaataaa tatttatttg ttaaagtaat taaacaatcc
     5701 gctaatcgtt tgatctggca tttctatttc cttaccatct aaagtaacag tagtaacgac
     5761 aaaatggtct tgcaatggcg gaacttctaa gtactgccct tgcagaaaat cattcactgt
     5821 ttcatattct tttttcattt cttttgaatc tgtcgatggt ttatagctta tttttaacat
     5881 aactagtgct cctttttatt cgggacgtgc aatcgtgaaa atttctccta ttgctgcata
     5941 atcatttcct gccaaacggc ttacagcatt caattttctt ggatcgatcc ttcctgtctt
     6001 atcatcatag actgtttcat ttaaatgata tttctgtact tctagcaaaa ataaatcagc
     6061 tgttacttta tttttattcc gtatctcgac atgttcatat aaggatgttt caaaacgaac
     6121 ttttgcttct gtcaatgctg gtactgaaat gattactgaa tcagttaaag tcaagtcagt
     6181 caggtacagt tcgctttcct cattaggcag attggctgct gttttattta cttcttctac
     6241 gttgtcctca tcaaggatat ggataacagc ttctcttgtc tcaagaatat tctttgctgt
     6301 gtcttttact gcatcttgtt ttctctggat cgccaaggat aagatgggtc gattcgaact
     6361 gacgatattg aagtaactga agggtgcgat attcactata tcttttgctg atttagtcgt
     6421 tactactgct actggtctag ggatgactga accaatcagc aacttgtaat tttcacgttc
     6481 tgtcaattct gaaggactcc gttcaaacat ggtttcgcct tctttctata aaattcgtta
     6541 aaaagcagat cttgaagaca aaatgaaaag taatgctttt ttcatcttct tagaatctgc
     6601 tttaaactat ctatcaattt ttcttttttg tcaagaatct gtgccgtgcg tgttttttag
     6661 ttatttgctt tacttgcggc ttcgacatat cttttagtga tcaattgcgg acgggtgata
     6721 atagatccaa ctacgactga aaaagcacct aattcatatg ctttctctac ttgttcagga
     6781 gagtcagtat gtccttccat aattactggg atatcgactt gttctatgat ttgattcaat
     6841 aaatcaaaat caggtgtctg cgtctttcgt gaatattctg tatagcctgc taaagtcgta
     6901 gacaaaagat caaaaccatc ttcttgcgct tgaagcgctt cctcaaaagt cgaaatatct
     6961 gccatagcta accgaccgtg ttcgtgaatc atttggacaa gttcagcagt tgttttttct
     7021 gtcggacgtt tacgagaagt tgcatccagt gcaatgattt ctgcttgcgt ctccaaaagt
     7081 tcttccactt ctttttctgt cgcagtaata aacacttctg acccatcgta atctcttttg
     7141 ataatcccta ttaccggtaa atcaacgatt cgcttgattt cattgatatc ttctgtcgta
     7201 tttgctcgaa taccaactgc tccgccttct tttgcagcca aagccattct ccccataata
     7261 aaagaactgt gtagcggctc gttttccaaa gcttgacaag aaacgacgac tccgcctttg
     7321 atttgttcca gtgtatccac aatatctcct ccagtaatgt tatataaaaa tacagcttca
     7381 tataaaaatg taaccctttt ctattcagga gacaataatg agctatttct ttcagcagac
     7441 ctttcttact aaaagaaaat tacttcaaat tcttcgtata cgaccagctt ctcttctgta
     7501 aattctatgt tcaacgcttc tttataagca tgtttaaaaa actcagcatg gactttccgc
     7561 caataagcta atgtccgatc cccttctcct tctttccaag catgctcttt cgtgacttca
     7621 ctaaaaggca agatggtcac atttatattt ttgatgatac aaactggttc attttttcca
     7681 ttcaggacca cgtctaattg accaactttt ggtaaaggct cattctcttg tttatacaga
     7741 tcgtaaccac ttgatgtacc agtctttatc ttttttacaa ctagttcgct caactcgtct
     7801 gccatttttt ctgtatcccc aaatgaaaag aaacctaatt tttctgattg ataactagga
     7861 tatttttctt ggaacttttt gacatattct tctacttgct gatacataac atccacccct
     7921 ttgcttttta taatgaaaaa accatccaga tactagtata aacaattatc tggatggtga
     7981 gcataatgaa ttattgaata ttcaagacac gcagactatc ttcaacgatt tgttttggtg
     8041 ttaaatgata acgttcataa agctgcccta caggaacgct atccgtgaat tctttctctg
     8101 caccaaaatt taatacgttc attgaagaat caccataata acgactgata ttttcgccaa
     8161 atcctcctga caaactacca tcttctaatg tgatgactaa ttcgtgtgtt tcttttaatt
     8221 tatctaatgt ttctacgtca ttttctgtga tcgttcctgg attgatcaac gttgcttcga
     8281 ttcctttttt tctaaaagct tttactgttt cttttcctaa ttcaagaaaa ccgcctaaag
     8341 ctaaaattgc tacttttcct ccattattta ctgtttgata ggctggaata ctataatcat
     8401 ttgcgatagc tgtaccatgt tctacaccat gtgtaggaat tcggattgct acaggctgcg
     8461 ctgtttgttc gaacgcccaa tctagcatag agatcatatc ctcttttgtt gctggagcaa
     8521 gatatttgat gttcgggata cttgataagt aaggaatgtc aaatgttcct tggtgtgttc
     8581 ggtcactagc tgagatcgtt ccgccaccga ctaaaataat cactggattc tcgttgatcg
     8641 ctaaatcatg tgataattga tcatatgccc gttgcaagaa agtacttgat tggaagacga
     8701 ctggacgtac tccctgagaa gccaaagcag aagcgaaagt gatagagtgc tgttcagcga
     8761 tccccacgtc aaaataatga tctggatgtt tttcttggaa tgttttcagt ccaaaggcgc
     8821 ctggtatcgc tgcattgact gcggcaatag gagctgtact tgtttgcatt ttcttatcca
     8881 aataatccat gatgatcata ttaaatgttt ctccagaagt tacttgcttt gcctccccgg
     8941 tggacagatc aaatggtaca tgccaatgat atttcatttt attatcgatt gccggctgat
     9001 aaccgcgacc tttttccgta tggacgtgaa gtacaatcgg atgatcaata tcttttactt
     9061 cttcaaataa acggatcacc gtttccagat cgtttccatt atccacgtaa cgataatcaa
     9121 atcccattgc tttaaatagg ttattgctgc ttttaccgtt ggtttgacga agctcagcta
     9181 agtttttata taaactgcca tgattctcat caatagacat ctcattatcg tttactaata
     9241 cgattagatt ggattgtaaa acaccaccat tgttcaatcc ttcaaaagct aagccaccac
     9301 ttagggagcc gtcaccgata aatgctacca catttcctga ttctttcttc agatctctcg
     9361 ctttagctaa acctgttgct aagctgatag atgtagatgt gtgccccact gtaaaatagt
     9421 catgtttact ttcatgttgg ttggaatatc cgctcacttc acggtaatgt cctttttcaa
     9481 aaccattttt tcgaccagtt aaaagcttat gaccataaga ctgatgggaa acatcccaaa
     9541 cgattttatc ctttggtgaa tcaaatacat aatgaaacgc aatcgttaat tctactacac
     9601 ctagattcgg accaacgtgc ccacctacat cgctgtcctt ttgcaagatt ttttggcgca
     9661 tctcttctgc caactgtttc atttcctcaa cagataattt tttgacatct gcggggctat
     9721 tgattgcatc caatacatgt aatactgtac tcataataca ttctctcctc cgtactatct
     9781 tcttttaatg aaaatagatt tgttacactt tatctattct actacttaga gttgactcta
     9841 agtcaatagc attttaaaaa aatttcagag aaagcttgtt atataagtag atcttcgcac
     9901 gaatcaggac tcaaataaaa accttctaca gacaattaaa aagctttgta gaaggtctcc
     9961 taattaaggt ctcatcaaga aatatgtttt tttaataggt gaagttcctg agcaatatga
    10021 tacaaatgga tgtttgcacc ttttaatgag attggataga tctttgtctt ttcagtttct
    10081 aatccatttt gatagctagc agcatcgcac aaaaccactc tttttttcgc acatagacat
    10141 tcatcaggca attcttctaa tgaacgaaat aaaataggat ttatttcagc gatgccttct
    10201 tccgcataca aatgaaattg ggttcgataa aaaaattgtt tgactgaact ttctgaaaca
    10261 acaaaaattt ctatttcctg tctccccgct tgttcactgt tgatcaaaga aagacttttg
    10321 acgaattttc gaatgaaatt ttcattccat cttagttgga tttgattttt ctttttccac
    10381 tcttccaata tttctacaat ttttccgtac agaagttgtt ctttttctga tagaagatag
    10441 atcctttccg gtaaaaatac ttgtccatct gcccacaacg tacggacaaa ttgtaaaaag
    10501 gattgcttga aaataaaatt ttctgcatcg tcgatttcag cgatactaat aatctgtaaa
    10561 atcaattcat tcagtgatag gtcattgaga acaaaatttt tataaacaac ttcgaaatct
    10621 tttcttaaca gctccatgtt ctcattggta taatttctac tgttgaacaa agaaaaaata
    10681 taatagacat cttcttcttg taagtctaga attccttttt gctcttccaa tccttgacag
    10741 actaattgaa ataacggctt cgtttctgcg cttttcttat cttcttcttt cagtccaagg
    10801 ggaaatgcct ttcttccgat tgcgatctct attcctcgta tgataaagga cttttcatct
    10861 tccgaatatc ttcttttaaa aaaatgagtc tctacataat caatcaactc gttacaagct
    10921 tcttctacat acggattttt attttcatag ccttcccaat tgacataacg tactaaagct
    10981 agcaagatgt tccgcgagtc aaattctggg ataatgattt ctttattttt caaataatca
    11041 ttttctttaa aaaaatccaa tacgattttt ttgattgtat atactttgct caaagaaata
    11101 aattcaagat cagccagttc tgtactcgtg aatataccat taaaataata acttaaaaac
    11161 tttaaaaaat aagattgacc gtacagaaac tgtgtgagct cagaaagagc aataccatgt
    11221 ttgaatacga gtttatactt atgcttctct cttacttgtt cttttataca atattctaga
    11281 cactcggcta gactttctag atcgtgaagg atcgttggtg tagtcaccct tagagaaata
    11341 gctgtttgtt cgatgtctat ttcttggaat tcaaataatt gttcacacag atacactttt
    11401 ctaaatatat ttcgctcgat gtaattttcc agcattcctg atacacgcct ccagtttttc
    11461 ttatgatttt atttaatcat atctgtaaat cgtatttttg tttccttttt atttagaaaa
    11521 gccctttcaa aaaccaataa aaaaagaaac tgggacaaaa gtcaaacaag ctcacaattt
    11581 cagcctcatt gtaggtagct ttgagccgtt tggactttta ccccagcctc tttcaagaat
    11641 ggttattcaa agatgtcagc gtctagttct ttgactacat ctttcaacaa ttggatacca
    11701 gctgcgctgt taccaaatgg atctagtgcc gggctgaaga cacctatgcc gtaacgattt
    11761 ggcacagctg ccatcaaccc gccgcctaca ccgctttttg caggtacacc aacatggacg
    11821 gaaaactctc ctgattcatc atacaaacca gcagttgtca taattgattt gacgattgta
    11881 gcactttctt cagtaatgat ttgtttgcca ttccaaggag caattccttt atttgccagc
    11941 acagcagcta attttgctat gcctgtagcg gtgacattga gggagcattg tctgaaataa
    12001 gcatcgagta tctccggtac gtctccttcg atcatttgat tacctttcat atagtaagca
    12061 agagagcgat tgatatcacc tgttcttgat tcagaatgat agatttcttc attcaatgtg
    12121 atattaggat catcacagat ctctttcata aatgacaaga ttcgctcaaa tttttcatct
    12181 gcatctttac cggcaatcaa cgaggttgtt gcaatcgctc ctgcattaac aaaaggattc
    12241 attggatgtt tgcgatgatt gatttccata ttcatgatag aattgaaggc aaaaccggtt
    12301 ggttccgtgt tgattgtttg aaagactttt tctattccgt tgtctttgat tgctaataac
    12361 aatacgggaa cttttgacat gctttcgatg gcaaagcgca catcggcgtc tcctccctca
    12421 atcagttgtt ttgtatccaa atcataaatc gccataccta attgtttggg atccacttca
    12481 gctaatgctg gaatgtaatc cgccacttgt cctgtttgat aatagtggaa attttttcca
    12541 atcaattgtt ctaattcttt tacttccatt tgaataacca gctttcattt tttatttttc
    12601 tgcatgctgg aatttatgac ggcatgcgta gatcgtaaat ggaataacta agacgataag
    12661 ataagaaaca atcagaatcg tttcataaat cccatgctgt ccagctgcga tactagcagg
    12721 tggaacaaat gagatacaaa aagcaaaaag tgaagtcaaa agacctaaac aagcaacgat
    12781 tacttttaca acttttccgc ctggaacctg ataggctctt tcaagactgt catctttaaa
    12841 aaccagcttc aaatagcccg cgaaaaacat aaagtacaac atcaggtaga ttacaacagt
    12901 caatgacatc gctgcaagaa atgaaagatt attccccccg ccgcttaatg taaagacggc
    12961 tgcccagatt gttgcaacga tcccatttgc gatcatcaaa tgagcaggaa caccgtgtgt
    13021 gttttctttt gcaaaatatt caggaagtaa tccttcttct gctgctacgt gaagtgcttt
    13081 tgaaggagag gttacccaag aagcaatttc tccgatgaca cctaaacaaa caagaatcgc
    13141 aagaattttc gcaatccatg caagatttgg atcaatgtgt cttaatagga attctaacga
    13201 ttgaatcacc cctgtattaa gagacaaacc atgttgaggt actgtagtag cgacggtcaa
    13261 tccgccaaaa gtatctaaaa tgattgccag aataaccaat acaaacattg ctaatggata
    13321 atccttttta gggttttcca tttcatttgc atgactggca gatgtttcta cgcccatata
    13381 tgctaaaata aaggaaacaa aaacaaccag cgtaggcaat ttcgaaaaat caggaatcaa
    13441 tgttttcata gacagtatcg tttgtagtgg agcgcctgaa gcgacataat ggatccctaa
    13501 gccaaacaag actaatgctg gcaacaagat accagctaca aaaccgatcc gagcaatcaa
    13561 tgaagtgtat ttggttcctt ttaactgtga gagtaaaata gcccagaaaa taatcaatag
    13621 gattgccaat ttcagatatt tattgttttg tatagcacca atacccgttg cataggataa
    13681 tgcaccagtt aagaaataca gcatggtcaa gtagccaact gttatctcaa accattggaa
    13741 aaagattgcg gcaaaaccga atcgttttcc caatgtgcga ctgacccaag tatagacacc
    13801 gcctttttcc catcctttca ccgttgccat ctctgctgcg cacaatgcca cagggataaa
    13861 ccatagcaaa ccgcctaata acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt
    13921 gggatattca tataatgaaa cgaccattga gatggtcata gccaaaaagc caaagaacgt
    13981 taattttttt gcaggaattt tcgttttgct ttgttgcatg aatcttcctc ttttctattt
    14041 gaatttgttt caattgagtt tgttgccaaa gaagaaaaag tccaatcctt cttcttgttc
    14101 ttcttttgta gtagtgatcg ttgtaaagtg catcgccgat tttaaaatca ctgttgtcag
    14161 cacataggca tacacggcag taatgatgac accaatcact tgaatcccta agaagtacca
    14221 gctgcttgga ttcgttagta aatggctgtt agatgcaaag atccctatca gaatcgtacc
    14281 tgtgaatccg cccatgccat gaacacccca cacatcaagt gcatcatccc attgtttctt
    14341 tttacgaaaa tcaacagcag catgacagac gatacccgca atgatcccaa tcagcatggc
    14401 actttttgct tctacataac cggcacaagg cgtaatggct gccaatccag caacggatcc
    14461 tgttagtacg tcgacaaaat caaagccatt tccttttact tttgcaatga tcagccaagt
    14521 aatcatcgca aatgcaagag cgatgaatgt tgaaccaaat gcattgactg cttgttcatc
    14581 tgctcgaagt gcaccaccgg aattaaaacc gaaccagcca aaccaaagca gtcctgtacc
    14641 gatagcagct gccataagac tgctatgctg acttctttct cctaaaggaa tctttctttg
    14701 accaagtgtg aggatacatg ctaaagagcc gaatccagct gtcgtgtgga tgactgctcc
    14761 accagcgaaa tctctaaatc ccatctgatc caaaaatccg ccaccccata tccagtggca
    14821 aacagggaaa taaatcagca gattccaaaa aataacaagt ataatatagc cttttaaatt
    14881 caatctccct gcaaaagctc ctgtcatcaa aggaacggta atcacgataa acatcagttg
    14941 gtaaagaaag aacattaaaa atggaatcgt tgcaccatga aacatgttgg ggaagcttgt
    15001 aacatgtctc atcaaaaaga aatctgccgg gtttccaata acaccgccga tatcccgtcc
    15061 aaaggccaat ccgaatccac caaataccca cataatcgtt gttatcccaa cggagagaaa
    15121 agattgagac atgatcgtta gtatattatt cttccgtgct aatccgccgt aaaaaaaagc
    15181 tagaccagga gtcatcaggc aaaccattgc tgtacataaa atcatgaatg ctgtatctcc
    15241 tgtattgata ggtaatgtat tcattttttt cgcctccttt ctacgattac gagtataaaa
    15301 gaaggtgtag ttgttttttt cttctgtttt tttgaattaa tacttgcatt tataaaagac
    15361 agacaattaa taaaagcttg atgtgttgtg ctctttttc
//
LOCUS       DARBFD010000062        15033 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000062 DARBFD010000000
VERSION     DARBFD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.062"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..1029
                     /locus_tag="SCK08_001899"
     CDS             1..1029
                     /locus_tag="SCK08_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEG1201767.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1085..1303
                     /locus_tag="SCK08_001900"
     CDS             1085..1303
                     /locus_tag="SCK08_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201768.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1374..2189
                     /locus_tag="SCK08_001901"
     CDS             1374..2189
                     /locus_tag="SCK08_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201769.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            2253..2438
                     /locus_tag="SCK08_001902"
                     /pseudo
     CDS             2253..2438
                     /locus_tag="SCK08_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
     gene            3012..3275
                     /locus_tag="SCK08_001903"
     CDS             3012..3275
                     /locus_tag="SCK08_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201770.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            3609..3980
                     /locus_tag="SCK08_001904"
     CDS             3609..3980
                     /locus_tag="SCK08_001904"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1201771.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            4160..4582
                     /locus_tag="SCK08_001905"
     CDS             4160..4582
                     /locus_tag="SCK08_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201772.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            4727..4972
                     /locus_tag="SCK08_001906"
                     /pseudo
     CDS             4727..4972
                     /locus_tag="SCK08_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5081..6037
                     /locus_tag="SCK08_001907"
     CDS             5081..6037
                     /locus_tag="SCK08_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201773.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(6873..7544)
                     /locus_tag="SCK08_001908"
     CDS             complement(6873..7544)
                     /locus_tag="SCK08_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1201774.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            7861..9492
                     /locus_tag="SCK08_001909"
     CDS             7861..9492
                     /locus_tag="SCK08_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG1201775.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            9495..10574
                     /gene="uxuA"
                     /locus_tag="SCK08_001910"
     CDS             9495..10574
                     /gene="uxuA"
                     /locus_tag="SCK08_001910"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1201776.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            10648..12075
                     /gene="uxaC"
                     /locus_tag="SCK08_001911"
     CDS             10648..12075
                     /gene="uxaC"
                     /locus_tag="SCK08_001911"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG1201777.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            12159..12806
                     /locus_tag="SCK08_001912"
     CDS             12159..12806
                     /locus_tag="SCK08_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG1201778.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            12935..14311
                     /locus_tag="SCK08_001913"
     CDS             12935..14311
                     /locus_tag="SCK08_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1201779.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            14322..>15033
                     /locus_tag="SCK08_001914"
     CDS             14322..>15033
                     /locus_tag="SCK08_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1201780.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDI"
BASE COUNT         5232 a         2034 c         2955 g         4812 t
ORIGIN      
        1 atgaatagca aaaaacttca gatgggtgaa attgttagag gtcctagtca aatatctatt
       61 gatgttacat ataaatgtaa ttttagatgt aaacattgtt acaatgcgag tggtggagac
      121 aattgtttta tggataatga gttgactgat gatgaactgt tagaacttac aaaagatata
      181 tgtaatttaa agccatataa tatttgtttc tgtggaggag aaactttact gagaaaaaaa
      241 atagttttat cttgtgccac tttattaaaa gaggcaggta ttcctaatat tgctattgtt
      301 actaatggat cattagtaac tcaggatgtt gcaaaagaat tattcaaagc aggtattaat
      361 cgatttcaag taagtttaga tggaaaaaat gctagtagcc atgaagcact tagattatat
      421 ccaaacagtt ttgaaaaggc aattaatgct atcaaattat ttagagaatt gtccgaagaa
      481 gtggatgttg ctttttgtcc tactagtttt aattacaatc aattacgaga agtatatcaa
      541 ctagtaaata ctcttaaagt gaagtcttta agaattcaac catttatgct tagtggtagg
      601 ggaatgcaaa atagggaaat agtgccaact gtttctcaat atagaaaact agtttctgat
      661 attaactata ttaaaagaaa tgaagattat accaagatag attggggaga tccaattgat
      721 catatcatcc gacacagaac tttattgaaa gatttttcga attttgtttc tattaggtca
      781 gacggaggat taatggcatc accttacttg ccaattcaag ttggaaatat taaatgtcac
      841 agtctttcta aatattggcg agcaggactt tataaagttt tttctttaga tttagttcaa
      901 gatatagcta ctcaagtttc ttcaatacaa gatatgagta aagaattaaa aaaaggacat
      961 attaaatggc ttaatgaaga tatagaaatt gatattctcg atgaatacaa acatgatatt
     1021 ttaatttagg tttatcattg gaaagtttag ataaacaaag tacttttaga aaggagggaa
     1081 aaaaatgatt tctgaacctc aaatgaataa tatgtatgaa agcccaacta taatgggagc
     1141 tggtgaagct gcgccaagag cttgttcagc aattatggtg tgtgtgttat tagcatctgc
     1201 aggagtaggt atccaagcag gagcggtgta tacttatgct gtagttgcta cagtagctgt
     1261 agctgcagcc gcagtttgga aaacagtagg attagttggt taggaattaa taggagacta
     1321 actgtctcct attgattcca atgattaagc taaggaggaa ttttaagaaa ataatgaaat
     1381 acaataattt aaaaccgaaa ttacttaatg agaacataga atatataaga gaagaaaatt
     1441 atggatattt aaccatagtt cccaaaaatg gaactgaatt gaaagataac gttttcaatc
     1501 caacagtaag atatatattg gctaactgta ctggaaaaaa cagtgtaaaa aaaatagcga
     1561 atggtttaaa aaagaagttt cctagtttag aatatgaaaa attctatcaa gatgttgtag
     1621 gaactattaa atttttcaaa cactataata ttattgatat aggagattat agtatggaag
     1681 aaaagaaaat atataaaatt gaacaaaatt tagaattaga attactaaat gaatcacatt
     1741 atgaagaaat ttataatttt atcacagatc atcctgaaag tatgtatttt catgaagata
     1801 tttatcatca aggaaatagt aaagaattag aagttgtcct tagatataat ttattttcgt
     1861 ttacgagtga attttacttt ttaagacata aagagagaat tgtaggaatg gtttctataa
     1921 ttgttaatcc atttcaaaac ttatcctaca gtatagtaaa ttacctcata tgtgagcctg
     1981 catatactac gactattttg acaacagtat tagaaaatat taacgaagtt tcaattttag
     2041 aagtaaagaa gctagtgatt catttgctaa attcaacaga agatgaatca ctaatcccct
     2101 atttaaaaga ttcacatttt tttaaagaag cggtactatc taaagaatat agagagcatg
     2161 atgttttgag atacgcttac atttattaaa taaaatttct gatagtattt taataattcg
     2221 gatcatatca taagatatag gagggaaaag aagtgatttt attgattttg tctctcgtat
     2281 tactagttgt atctaatcta atgaaaaata atcctcctac aaaaattaat tctttttatg
     2341 gctttagaac taaaaagtca atgaaaaatg aaaaaagttg gcatttagca caaaaattgt
     2401 tgggggattt ttcaaaaaaa aaagttatcc cagtattttt gatgggtatt gtttttttca
     2461 gtatagagat ttatagttta gctattttaa aaaatgaaaa ttttttcgtt agtgtattaa
     2521 tgggtgaaag tttaatccta atactgattt tgtgtttaat tgttcatgaa gtgaataaac
     2581 aattgtgaat taaaaaaata aaaacctagt ataatcactt taataaattg ttggtagaag
     2641 gtgatcaaaa tgggagtaaa cgaaatgatg aaagtcagat tgagttttta aatgatacct
     2701 tcctcagaac ttcaaaacga attcaaagat tcgttgacaa atcttgagca agatcattta
     2761 aaaaaatatt ttctcttcat ttgacgaaaa atgtttagaa atcttatata agtgaagata
     2821 aagtaattgg tgtaattaat tgtagagaat agcctttgag caattgaagc gatacaatta
     2881 tttagaaaat gaacttcctc cagcatacta catgattcct tacgggacga tgttaaagaa
     2941 gaaagcaaga aattttagaa gtattagtag ataaactaat gggcttttct caatttaagg
     3001 gagtagccca aatgtttatc aaatatggtg cagatacagt agcaaccatg tttacagaag
     3061 aatatcaagt agactattat gaagtaatag ataaatgtgt acattttgtt tatgaaaaag
     3121 tgtggaattt tatgaatttt gcgataagtg aagaaatctt acgagtacaa gaaaacagaa
     3181 caggaatatt taccagagac agaaagctta atttgattgc gcattatata cggaatcgct
     3241 ggtcatcaat accaaatttt aattataaaa aataattatg aacaatcttt tatacaatat
     3301 acacaataaa tcaagtagta tttatgaaaa aagacttcaa aaacagaatg attttctatc
     3361 agtcttagaa attatagagc taaaaatttt ggtcacgcgt agaaacgcgt tttttttgtt
     3421 tttttgatag taaagaacgt acaaaacaat gagaatttaa aagattgtac taccagtttt
     3481 tttacctttt catttgagtt tagagtaagt aaaatattac tataaaaaca catattcttg
     3541 aagaaatctt ttttttgctg tattcattta gattatgata gagaaaatac gtgagagggg
     3601 gacctgggat gtggctatct gatgaaaaaa ttcagtggcg tagctatcag aagcgtatat
     3661 tgactaactt tgctacttat ttaaataaca attcagtcca tctagtggct ccaccagggg
     3721 ctggcaagac ctttttagga attgagatga tccgacgttt ggattgtaaa accttaattt
     3781 tggttccttc cctaatattg aagaaacagt ggttaacaac aatcgaggaa tatttcttag
     3841 taaatccagc agatagttgc tatctttctg gggcgattga acatcctagt catattacag
     3901 tggaaacgta tcagaccatt tacaaccgac tgaaggagga gccaacttat ggtgatcagc
     3961 tggagctgtt agttttttga tgaagctcat catttaaaaa aaagctgggg tgaattgttg
     4021 attgacttga agaaaaaaca accgaatctg ttgacgattt ccttgacagc cacgcctccg
     4081 attgatagct ctcagacgga gtggaagcaa tatattgagt taaaatggcc tgattgacga
     4141 agaaattagc attcccgagt tggtaaagga ggaggttcta tgtcctcatc aggattttct
     4201 atatttgatt ccggctaatc aggaaactac tcagcattat tttacctttt tagagaaaca
     4261 aaatgagctg gtggaagcgt taatggaaag ccaagagacg gtggattatt tgctttcttt
     4321 agaatttgtt cagcatccgc ttgagcattc gaaggaaatt tatgaaaatt ttgagctgtt
     4381 cactagtgca ttaatttact tgaatcatca taactatgaa ttgatacccg aacattggca
     4441 gctattaggc agttctaagc agcaaggtaa aaaagcttca gttccatctt taggtaaaaa
     4501 agagctgatc actatttatc agcagatgtt tgaagatcaa ccggaattgc ctatttttta
     4561 tcaattgaaa aaagctggat gattgtttga tgagtcgctg catttgtttc cagagtatcc
     4621 agacactgtt ggtagtcagg cgattcctga gaaaaagcaa gcagtttgtc aaattttgtt
     4681 aaaagaagaa gttcagctga agcaagaact gacagcggtc attttgatgg atcgtatttg
     4741 caaggaggta ttagcaggca gtgatggtac cttagcttat ggcgtggttc ctttgtttga
     4801 tgaagttgtt cgtctattag gtaacactca gcaaatcggt gccatctgtg gagagttctt
     4861 actgttaccg aaaatgctag taactgttga gctggcagaa ctagcgacgg gtatgaatga
     4921 acatccttat atgtcgggtt atttttattt gacggtggat gacaaaaacc gccaagcatt
     4981 gatgcaagca gttactcgcc tattaaccga aaagaagttg aatattttgg taggaaccgt
     5041 tagtttgcta ggagaaggct gggattgtcc tgcaattaat ctgttgatct tggctaatca
     5101 agcttccact tttgtgcaaa cacaacaact acgagggcgg ggcttacgcc taggtgcaca
     5161 aaataaagta acgaacattt ggcatttagc gattggatta ccgggagttc ctctgtctga
     5221 acagcctgat ttgaaaagaa tgctacggcg tttgggcaat atttcaggtc tgacctttga
     5281 atcttcacca aaaattgaga gtggtggcga tcgctttgct ttaccactgg aatatacgac
     5341 caccacgatg gaaacttata ctcagtcgat gttctatttt tcttgtgaac gaagtcggtt
     5401 gaaagaacaa tggagtactg ctttagctga tggctggcac ctatctatgc caatcgtcgt
     5461 taggaaagtc gatcaggtaa agcaaaaaca agaaacttca ttgccgatag tagtaaaaaa
     5521 tatagagcct ctaagcttct ggtcagctat atttaaaggt aacttgctgg aatatttcca
     5581 aattcgtagg cgacaaaagc tatgggaaaa ggaatgtgcc cgcaaaaaaa gcttggccat
     5641 atgcgttcaa acgacgttgc agcgccttgg tgaagttagt gatagaaaag taatcgtagc
     5701 ggctgatcag caggaattta gatgctatct tgagaatgct ggctatcgag aaggacagct
     5761 ttttactgaa gctatgaggg agcttttagc accgctggag aatccgcggt atttaatcga
     5821 agggaaaaag atagttctga cggtaccaga acgctttggc cgaaataagc aaatcgctaa
     5881 cctttttcta aacgaattaa atcaatcgtc gctgcccaaa tgtcagttgg tgtatactag
     5941 aaatcctgaa ggacgccaaa agctagtgac ttatcgaatg aggcaagtct tccagacccg
     6001 cgatgaagta attattcaaa aaaacatttg gacgtaagtt gatgaaaata aattttgcta
     6061 gagtagaaca agtccgagaa ttaagttgct acttaactga taatgccaag gacaggagaa
     6121 tcttttcttg acgactgaaa gaaactattt gacttatata acggtgtgtg atgaatattt
     6181 gcaaggcatt ctcttaaaaa gtactcgtaa tagttttttt gaaaacaaaa cagtacttta
     6241 ttaataggac tgacagagcg atataagaac atctgcagac agaggtgaat gcttttctca
     6301 tgatcgatga aacaatggta cttcggatat tcttgctttt agtttgaacc gttgtgattt
     6361 tctttgattc ctaaaagtag ttttatagaa tgaaaatcaa gcatacggaa tgctcctttg
     6421 attgtgttgt ggtgattcaa tctaatggag cttctatatg tttttttatt tgcaaaaaga
     6481 accgatagaa tatttttatc agtcttagaa attatagagc caagaaattt caagcacaca
     6541 taaaaacgcg tgttttatgc attttatgag caaaaataac taaagaccat tacgtaaaac
     6601 ataggtaaat cagcattcaa tctcaaatta tgtgatcaaa tgttgatatt atttgattat
     6661 aagttttctt ttgctattat cagtctgcaa ataaacagat tatagagtat gaatgtagat
     6721 agttcaatct ttgtttacga cctcacatgt ttttttactc aagagaaagt aatataaagt
     6781 gactatattt gacagaactt tacataaaaa aagtagttag ttttctgtag taatcagaaa
     6841 tgtaaataaa ttaaaaaaaa gaaatttagc agttatatat ttttaaaata ttttgggtga
     6901 gtagtcaata caacattttg ttcatcaagc attaaatgaa gatgcaagga tgttaagaag
     6961 ttagcttctt cagggtcttg catggtcaag gctttaaata gttgatgatg ttgttccata
     7021 attttttccc attttaatcc ttccgttttt actcgtaacc aacgaaatcg ttccaagtgc
     7081 gtattattat cttccaacca ttgccatact tcttcccgtt ctgcaatttc aaaacaagct
     7141 ttatgaaata aattatcatt atgaaaaaaa ctacgagcat tcttttgact aacagctttt
     7201 tcttgttctg caattatagt ttctaaaact tgtttctttt gattattcat cttggcacaa
     7261 cactctagca taatttgtcg ttctaaatgt tctctgacaa aacgagcatt ttttgctaaa
     7321 tctaagtcga ttaatgagat ataatttcca ctttgaggaa ttgtatcaac aagtccttga
     7381 tgacggagtt gaatcaatgc ttcacgtatt ggagtacgac cgatagaaag cgtttcttcc
     7441 aatttttttt ctgatacttt ttttccaggt tctaagtcag cgtagataat ttgctgacga
     7501 atagattgat aagcttgatt ttggagacta gttttaattg tcatatttaa tccctctcaa
     7561 aatttcattt ttgaatttga tattttagtt ctatgctcgt tatataatat caaatttcaa
     7621 agtaacatac tagctattaa aaaaacaaaa aatactcaaa taagatcaaa gagaaattaa
     7681 gctttaattt aagttagttc taaaaaatat atattggtgt gcttttacta aaaaaagaca
     7741 caataattct gacatggtag aaaaaagaat atttttagtt ttttcttatt gacagcgatt
     7801 acattcgatt ttatactaca attgtaacgg aaactaacat atcagtatgg aggggtaaat
     7861 atggttttga ttaaagactg gcaagattct atcgaaggat tgaacaaaat gcaagttcaa
     7921 ctaccgtatt ttaatgtcga agcggtcaaa caagaaggtc agaaacatcc agtgtggctt
     7981 cattttggtg gaggaaatct ttatcgagga tttcatgcag aaattgctca atgcttagca
     8041 gatcaaaatg aattagaatc cggagtagtt gtgtgtgaga catttgatga agaagtagta
     8101 gaaaaagctt atcatgacta caacaatgat attttggaga ttgttatggg tgaagatggt
     8161 gaactcaaaa aaagactttt agcagtaaca gctgatagtt tatattgcaa tccaaagaat
     8221 ataacggact atcaaagagt ggttaaatat tttgaagatc ctacattaca gtttacaacg
     8281 tttactatta ctgaaaaagg atacagttta aaagattcta aaggacaatt tttttcggta
     8341 gtggaagcag atataaaatc tggacctacc aatcctcaac atacgatgag tattgtgaca
     8401 tccttgttgt atagcaggtt taatgctgga gagttaccga ttgcgatggt ttctactgat
     8461 aatttctcac agaatggtca acgttttcaa gatagcattt ttatgattgc agaagcttgg
     8521 caaaaaaaag gatacgtttc tgaaaaattt attgcttatt taaaggataa agaaaaagtg
     8581 acttttcctt ggagtatgat tgatagaatt acgccaaatc cttcggaaag tgtcaaaatg
     8641 gatttattga aggcgggttt cgaagactta gatataattc atactgcgaa aaataccaat
     8701 atcgctgctt ttaccaatac tgaaaaagtc cattatctag tcattgaaga tagttttcca
     8761 aatggtcgac ctaaactgga agcggctggt gtgattatga cagatagaga aacagttgat
     8821 aaagcagata tcatgaaggt tacgacatgt ttaaatccgc tccatacggc attagcaata
     8881 acaggttgtt tgttggggta taaatcaatt caggcagaaa tgcaagatga tgatttggtt
     8941 ggcttaatta aagggatagg ttatgtagaa ggcttaccag tagttgaaag tccaggcgtt
     9001 attgatccaa aacaatttat tgatgaagta atcaataaac gtttgcctaa tccctatatt
     9061 ccagatacac cgcaacgaat tgcaacagat acttctcaaa aagtagcgat tcgctatggt
     9121 gaaacaatta aaaagtatgt cgaaaaagac ggaaatgcag aaaatctaac atttattcct
     9181 atcgctattg ctgcttggtt acgttattta ttggcagtcg atgatcaagg aaaaaatttt
     9241 gtgccaagtc ctgatccttt attggtagag ttgcaggaaa aattctcagt attgaaatta
     9301 ggtgaacaag atcctgcaaa gattcattgt gcggtacagg aaatcttaga aaataaggaa
     9361 atatttggtt tagatttata tcaagttgag ataggaaaga aaattgaaaa aatcttttgc
     9421 gaaatgctag taggtcctca agcagttcgt aaaacaatca ataaatatat aacagaagtt
     9481 ggaggaaagt aactatggaa atgactttta gatggtatgg ggcaaatgaa gaaaaaatct
     9541 cactacaaga aattcgtcag atcccagcaa tgaaaggaat tgttggaaca ttaatggata
     9601 taccagccgg agaggtctgg ccgagagaac gaattaaagc tttaaaagat gaagtcgaag
     9661 ccaatggcct taccttgaaa gttattgaga gtgttaatgt tcatgatgat atcaaaattg
     9721 gtttgccaac acgagatcaa tacatcgaga attataaaca gacgattcgc aatttggcag
     9781 aatttgggat tgaggttatc tgttataatt ttatgccagt ctttgattgg gtaaaatcag
     9841 atttggatta tcatttagat gatgggtctt ctacgttagc ctatatttca aaaaatattc
     9901 ctactgatcc agaggaaatt attagaaaag tagaaaattc tactaataat tttgaattgc
     9961 cgggttggga acctcatcgg ttatcacaaa tcaagcaatt attagaagcc tataaagaag
    10021 ttgatgaagc gaaattgcga gagaactttg catattttct caaagcaatt atcccgacat
    10081 gtgaagaagt tgggattaaa atggcagttc atccagatga tccaccatat tctattttcg
    10141 gattacctcg tattgttaaa aatcgtgacg atttagaatg gttgtgtaat gtagtggatt
    10201 ctccttctaa tggtattacg ttatgtactg gaagtattgc agaacgacca gataatgatg
    10261 tctatgaaat tctatctgaa ttttgtcaac gggatcggat tccttttgca catgttcgta
    10321 atatcaaata tttaagtgat aatggactcg atttttatga agcgccgcat aaatcagaat
    10381 atggttctct agatatgtat aagatattaa aaacaatgta cgataatgga tttaatggct
    10441 atattcgtcc agatcatggt cggatgattt ggggagaaac cggtagacct ggttatgggt
    10501 tatatgaccg agctctaggt gcagtttatt taaatggtat ttgggaagcg cttgaaaagg
    10561 gggcgagtaa ataattatat aatggagtct atttagaaga aaaattttct cttctaaaaa
    10621 ctaagggaag gaaaggagtg cttaaatatg tttttagata agaacttttt attagagaat
    10681 gaaatagcta aaagactttt tcataaattt gcagaacacc aaccaatcat cgattatcat
    10741 tgtcatttag acccagcatg tttgctagaa aataagcaat acaagaattt gactcaggta
    10801 tggttaaatg ataaaggagc tggagatcat tataaatggc gattgatgcg agctaatggg
    10861 atagaagaag agttgatcag tggtgcaggt aatgattatg aaaaattttt agcttttgta
    10921 aaaacagtgg agaaggcgcc aggtaatcct atttttgaat ggtctcattt ggaattacaa
    10981 cgttactttg atattgattt gacaatttct gagaagaatg caccagaaat ttggcgacgt
    11041 gccaacgaaa aaatccaaac ggattcctat cgcccaaaag aattgctgaa gatgatggat
    11101 gtgaaagcca tttgtacaac ggatgatcct acagatgact tgcgatatca tactgagctt
    11161 tctaaacagg ataattcttt caaggtatta cctactttcc gtccagataa ggtctggagt
    11221 atcaaatcat caggattttc gaactatatt ttaaaactta gtcaagcagc gggtatacca
    11281 atcaaaaact ttaatgggtt gaagcaggca ttgagccaac gagtggatta ttttcatcaa
    11341 attggctgtc gattagcaga tcacggtttg aatacttatt tttatgaaat agcagatgaa
    11401 aaaatactcg acaatctgtt aataaaagct ttagcaggaa aaacggattt ccatttatca
    11461 gaaattgcac aatttgttac aggtattcaa ttacatttga tgtctgagta tacaagatat
    11521 ggttggacga tgcaaatgca tttaaatgct ttaagaaata attcaagtat attaaaggaa
    11581 caaattggag tagatgtcgg tggtgattcg atgggggatc aagctagttt gacacaaaat
    11641 gttgtagcat tgttcgacac tgctgaaaag agtgggtgct taccaaaaac aattttatat
    11701 tcattaaatc caaccgattg gcttccacta gcaacagcaa tgcaaagctt ccaagggggt
    11761 atgattcaaa aaatccaatt gggatgtgct tggtggttca atgatacagc tgaaggaatg
    11821 cgtcaacagt tgactacgat ggcgcaacaa agtttgttgt cgaattttac aggtatgttg
    11881 actgattctc gaagttttct aagctatcct cgccatgagt atttccgtcg agtattatgt
    11941 caattactaa gcgagtgggt taaagcaggt cgaattccag atgatgaaat gtatcttagt
    12001 cagatagtgg agaatatttg ttatcgtaat gcgtataatt attttaattt ttttgaaatc
    12061 gaaagtagaa aataatacaa aaaataaaag cttaaattta tttattgtaa gttagtacaa
    12121 ctagctagag tatgtatcca taaaagaagg agatagatat gaaaaaagta gaaattttaa
    12181 gtaagttaga agcggctgga gtcattgcgg ttgtacgtgg aaaaaataaa gatgaggcca
    12241 ttaaagcaat aaatgcaatt actgcgggtg gaattaaagg gattgaacta acttttacag
    12301 ttccgaatgc tgaagaaata atcaaagaaa ttgctaaaaa atatgctgat caaaaagatg
    12361 taataattgg agccggtaca gttttagatg cgtttactgc tcgtattgca attatggctg
    12421 gagcagaata tatcgtaagt ccatcttttg atcgacaaac agcagagatt tgtaatttat
    12481 atcaaattcc ttatttacca ggttgtatga caattactga gataaaggag gcacttaccg
    12541 ctggagtaga aatcattaaa ctatttccag gttctgcttt tggtccatct gttgtaaaaa
    12601 cgtatcaagc tccattacca tatgtaaata taatgcctac tggcggagta agtctagata
    12661 acttagaaga gtggtttgaa gcaggcgttg ttgcagttgg tgtaggtggg aatttgttgg
    12721 ctcctgttga aaaaggtgat tttgaagaag taactcgaat agctagtaaa tatatagata
    12781 agttttatga gataaagaat tattaggaga aaaaataaat aatactataa aatgtaaaaa
    12841 ataaatacta actctttata cttcagagta tagtacgtaa gttggcagtt caacagaatg
    12901 aaaacgctta acaataggaa ggagcgcaaa ttatatggaa agaaaatttg gtctaagaga
    12961 caaaatagga tatatgtttg gagattttgg gaatgatttc actttcattt ttgctagttc
    13021 ttttttaatg attttttata caaaagtatt ggggataagc ggagctaccg taggaacatt
    13081 atttttagta gcaagattta ttgatgcagt tacagacata acaatggggc gtattgtgga
    13141 tggatcaaaa ccatctaaaa ctggcaaatt taaaccgtgg attttacgga tgagtggtcc
    13201 agtcgcttta gcaagttttt taatgtatca aacatcaatg gcatcagctt ctatgagtat
    13261 taaagttata tatatgtatg taacatatat actatgggga agtatttttt atacatctat
    13321 taatattcct tacggttcaa tggcatcagc tatcacttct gttgcagaag aaaggacagc
    13381 gttatcaaca tttcgtagta taggagctac cttagcaggt atgttcattg gcgtgttaac
    13441 accaattttt atttatacaa gagatagttc tggagctcaa attgtacgtg gtggatcaac
    13501 ttttacaatt gtggcaggag tattttcagt gtgtgcagtt atttgttacg taatatgtta
    13561 taaattaact gtagaacggg ttaaaagtga tgaatcgcag ttaaaagttt cattgttgaa
    13621 atctttaaaa ggattagcta aaaatagagc gttgctttct ataatagctg cagctatttt
    13681 tttgttgctt gctcagcttt taacaatgag tatgaacaat tatgtatttc cagattatta
    13741 taataatgca cagggaatag ttttaatgaa ctttatcaat cctattttga tgttggtact
    13801 tgtttcacct gtaagtttaa ttttcagtaa aaaatacggt aaaaaagaat tggcgtcagt
    13861 agggatgttc ttctctgcta ttgtatattt acttttattt ttgataaagc ctagtaatat
    13921 gtatgtattc ttagttctta cttctgttgg atatatgggg ttaggtattt ttaatacagt
    13981 catttgggcg aatattactg atgtaatcga tgatcaagaa gtgaaatctg gccaaagaga
    14041 agatggaact atttatgcag tatattcttt tgcccgaaaa attggacaag ctttagctgg
    14101 cggtgtcgga ggttggactc tttctataat agggtatgat tcgttagcaa aagtacaaag
    14161 tggagaagtt ttacaaaagc tgtataactc ttcaacgttg attccagcag tttgtttctt
    14221 tgttgtagga ttgattttat ttttcgtata tccattatct aaagaaaaag tattagaaaa
    14281 ttataatttg ctaaatcaaa aacataacta ggagaaaaaa catgaacagt tggatatttt
    14341 gtattgattc agatggttgt gtcatggata caatgactta taaacacaat ctctttttcg
    14401 ggccaattgc tgcagagatt tttgatgttc atcataggga aaaattccta caggattgga
    14461 ataatattaa tttatattca gagactcgtg gaataaacag atttttaggt ttagttaaaa
    14521 cattagaaaa aaatgatgta ccatcgatta atcgattaaa gcactgggta tctgtaacaa
    14581 aagaattgtc taatccatct ttgaagaggg aaatagaata ctctcagtca gaggatttat
    14641 taaaagcttt aaaatggtcg gaagaagtca atcgacagat tagtcaagct aaaggattag
    14701 ataaaccctt tttaagtgct agagatacta taaaagctct gaaaaaatat gggaagataa
    14761 ttattgtaag ctcagctaat aaggaagcta ttcaagaaga atgggaaaga catgaattat
    14821 tatccttagt tgatgaactg tgttgtcaag ataaaggaaa aaaagaagac attattcgaa
    14881 gtgtaattga aaatggttgt gatttggata agatattaat gattggtgat tctcctggtg
    14941 atctagaagc tgcaaataag aataaagttt tcttttatcc tattttggtt aataaagaaa
    15001 aagaatcatg ggaaaactta agaactgata ttt
//
LOCUS       DARBFD010000063        14815 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000063 DARBFD010000000
VERSION     DARBFD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.063"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(296..1024)
                     /locus_tag="SCK08_001915"
     CDS             complement(296..1024)
                     /locus_tag="SCK08_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEG1201781.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="SCK08_001916"
     CDS             complement(1002..2576)
                     /locus_tag="SCK08_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1201782.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="SCK08_001917"
     CDS             2805..3743
                     /locus_tag="SCK08_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1201783.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="SCK08_001918"
     CDS             3755..4666
                     /locus_tag="SCK08_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1201784.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="SCK08_001919"
     CDS             4690..6162
                     /locus_tag="SCK08_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1201785.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="SCK08_001920"
     CDS             6213..6824
                     /locus_tag="SCK08_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1201786.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="SCK08_001921"
     CDS             6854..8767
                     /locus_tag="SCK08_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEG1201787.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="SCK08_001922"
     CDS             8895..11876
                     /locus_tag="SCK08_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEG1201788.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="SCK08_001923"
     CDS             11873..13534
                     /locus_tag="SCK08_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEG1201789.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="SCK08_001924"
     CDS             13574..14506
                     /locus_tag="SCK08_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEG1201790.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DARBFD010000064        14574 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000064 DARBFD010000000
VERSION     DARBFD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.064"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            40..1806
                     /locus_tag="SCK08_001925"
     CDS             40..1806
                     /locus_tag="SCK08_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEG1201791.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            1834..3642
                     /gene="uidA"
                     /locus_tag="SCK08_001926"
     CDS             1834..3642
                     /gene="uidA"
                     /locus_tag="SCK08_001926"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEG1201792.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            3814..4215
                     /locus_tag="SCK08_001927"
     CDS             3814..4215
                     /locus_tag="SCK08_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1201793.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            4209..4454
                     /locus_tag="SCK08_001928"
     CDS             4209..4454
                     /locus_tag="SCK08_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201794.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(4778..4999)
                     /locus_tag="SCK08_001929"
     CDS             complement(4778..4999)
                     /locus_tag="SCK08_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201795.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(5161..5233)
                     /locus_tag="SCK08_001930"
     tRNA            complement(5161..5233)
                     /locus_tag="SCK08_001930"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5198..5200),aa:Lys,seq:ctt)
     gene            5309..5539
                     /locus_tag="SCK08_001931"
     CDS             5309..5539
                     /locus_tag="SCK08_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201796.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            5583..6752
                     /locus_tag="SCK08_001932"
     CDS             5583..6752
                     /locus_tag="SCK08_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1201797.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            7326..7895
                     /locus_tag="SCK08_001933"
     CDS             7326..7895
                     /locus_tag="SCK08_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEG1201798.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            7882..8742
                     /locus_tag="SCK08_001934"
     CDS             7882..8742
                     /locus_tag="SCK08_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1201799.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8932..9636
                     /locus_tag="SCK08_001935"
     CDS             8932..9636
                     /locus_tag="SCK08_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1201800.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            9641..11476
                     /gene="walK"
                     /locus_tag="SCK08_001936"
     CDS             9641..11476
                     /gene="walK"
                     /locus_tag="SCK08_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEG1201801.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            11473..12789
                     /locus_tag="SCK08_001937"
     CDS             11473..12789
                     /locus_tag="SCK08_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201802.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            12790..13659
                     /locus_tag="SCK08_001938"
     CDS             12790..13659
                     /locus_tag="SCK08_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEG1201803.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            13714..14523
                     /locus_tag="SCK08_001939"
     CDS             13714..14523
                     /locus_tag="SCK08_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1201804.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
BASE COUNT         5213 a         2185 c         2950 g         4226 t
ORIGIN      
        1 ttttggaaaa atcttactaa taagtagagg agaaagaaaa tgactataaa agtagactta
       61 cgaaaaaaac catatcattt agatgaagat gctatttctt ggattgaaga gacgattgat
      121 gctatgactt tagaagaaaa aataggtcaa ttatttgtta atatgggatc tagtcgtgat
      181 aaaaattact taagtagtat ggtcaatcag taccatattg gagctgtacg atataatcca
      241 ggaactgctg atgaggtata tgatcaaaac tatatcctac aaacagaaag caaaattcct
      301 ttattaattg ctgctaatac agaagcaggt ggtaacggag cttgtacaga tggtaccgaa
      361 gttggtgtag aagtaaaaat tggtgcaaca aatgatgcga aatatgctta tgaattaggg
      421 agagtttctg gaattgaagc atcggctatt ggatgtaact ggagttttgc tcctattgta
      481 gatattaata gaaattggag aaatcctatc atttctagtc gatcttttgg acaaagccct
      541 gataaagttt tagaacttag tttagcttat atgaaaggta ttcaagagag tggaattttg
      601 cctgcagcta aacatttccc tggtgacggt attgacgaac gagatcaaca tctatctttt
      661 agtattaata gtttaagtac agaagaatgg gataattctt ttggaaaagt atactcaggc
      721 ttaatagaag ctggattacc ttcaattatg gcaggtcata tccatatgcc agcttattca
      781 aaacatttta atcttgatct gaaagatgaa gatttacttc ctgcaacttt atcaaaagaa
      841 ttaatcactg acttacttag aggaaaactc gggtttaatg gattagtagt tactgatgct
      901 agtcatatgt taggaatgac tggggcaatg aaacggtcag aattattgcc cacttctatt
      961 gcggctggat gtgatctatt cctatttttt aatgatccag atgaagactt taattatatg
     1021 atggaaggtt attacaaagg aattatttca gaagagcgtt tacgtgatgc attaagaaga
     1081 attttaggct taaaggctaa actaggtttg cataaaaaag aaaagaataa aattttgtta
     1141 ccgaaacaag aagctatgga aaaaattggt ttaattgaaa ataaatcaat ttttaaagag
     1201 gtagctgatc aagcaattac actagttaaa gataaacaag atatttggcc tattgtacca
     1261 gaaaaatata aaaggatttt actggtagat gtacatgggg taaaaggtgg gtttggtgaa
     1321 gtagttgctg gaaatagacc tcaggccatt gatttattga aacagatttt gtcagcaaaa
     1381 ggatttgaag ttgaaaaata catttcacct gaagaagaat tgaaaggatt atcgcaagaa
     1441 gaaaaaatta aaaatatagc tggaatatat gctgctaaga gaccaatcaa agatttaact
     1501 gaaaaatatg acttgattat taatatagct aatgttaacc caggtacagt tcaaagaatt
     1561 gtttggccag caagtaaagg tactccggat attccatttt atattcatga aattccaaca
     1621 atttttgtat ctgtacagtg tccgtttcat ttagctgatg tgcctcaagt aaaaacttat
     1681 atcaatactt atgatggaaa acaagaaact ttagaaatat tagtggacaa actaatgggc
     1741 ctttctgaat ttaagggaat aagtccaata gatgcttatt gtgggttaaa ggatacattt
     1801 atttaatatt taaattatat aggagtgatt tacatgttat atcccattat gacagagacg
     1861 aggcaagtaa ttgatttaag cggtctttgg agatttaagt tagatacaaa tgatgactta
     1921 caagaagaac ttgctttaga accaatggtt gaaaaagaga gttatccaat ttcagttcct
     1981 tcttcttata atgatttatt tgaaacagag aacattagaa atcatgttgg atgggtgtgg
     2041 tatgaaaaag aagtgattct tcctaaaaaa ttattagaag aaagattagt tcttcgtttt
     2101 ggatctgcaa cacatgaagc aaaagtattt ttgaatggaa aattgattaa tcaacacaaa
     2161 ggagggttta cgccatttga atcagaaatt aatcagttta taaaagatgg aagtaacaga
     2221 attacagtgg ctgtaaacaa tgttattgac gatagcactt tacctgtagg gattttgaaa
     2281 gaaacgatac aggaagatgg agacataaaa aaagaaaatt tggttaattt tgactttttc
     2341 aattatgcag gcattaatag acccgttaaa ttgtatacaa ctccgaaaga ctatataaag
     2401 gatgtcacta ttaatacaaa aataattgat tcagatgcaa atgtagaatt taatatcgat
     2461 acagaatgga ccggagaaat tctaatagca atctatgacg aagaagctca acttatagca
     2521 gaagctaagg ggaaagaagg agtgattcaa cttaagaacc caacactttg ggagcctcta
     2581 aatgcttact tatatagtat gaaagttagt ttaattaacg aaaataaagt gattgatacg
     2641 tacacagaag aatttggcat tcgtagtatt caggtttcga atgggaagtt tttgattaat
     2701 aataaacctt tttactttaa gggatttggg aagcatgagg acacgtatat taatggtcgt
     2761 ggtttgaatg aagcagtaaa tatcaaagat tttaatttaa tgaaatggat cggtgctaat
     2821 tcatttagga cttcacatta tccttattca gaagaaatta tgcgcttagc agatcgagaa
     2881 ggtattgtag ttattgatga agtacctgca gtaggattac atttgaattt catggctact
     2941 ttattagatg acaacgtaga aaaacatgat acttggaaag aaatacggac agaaaatgct
     3001 cacaaaatgg cgattaagga attgatagaa cgcgacaaga accatccgag tgtcgtaatg
     3061 tggtctattg caaatgaacc agattcggat tctgaaggcg ctaaagaata ttttgaacct
     3121 ttagttgaat tgactaaaga acttgaccct cagaagagac ctgtaacaat cgttacttac
     3181 ttaaagtcaa caccagatat ttgtaaagta ggagatattg tcgatgtttt atgtttaaat
     3241 cgctattatg gttggtatgt ttcaggtgga gacctaaaac atgcaaaata tctgttgaaa
     3301 aaagagctag atggatggat ggaaagatgt cccgatacgc caataatgtt tacagaatat
     3361 ggagcagata cagtagctgg acttcatgat acagtaccaa caatgtttac agaagagtat
     3421 caagtgggtt attacgaagc aaatcacgaa gtaatagata aatgtgacaa ttttgttggt
     3481 gaacaagtgt ggaattttgc agattttgaa acaagtcaag ggattttaag agtgcaagga
     3541 aataagaaag gaatctttac tagagataga aagcctaaaa tgattgctca ctatctaaag
     3601 aaacgatggt catcaatacc aaatttcagt tataaaaaat aatttacctt ttataaggaa
     3661 tataaaaaag gagtggctgc ttaattgcca actccttttt tttataaaaa gattttctgt
     3721 ttaaagaagg atattttttt ctagtttttt cgaaaaaaag gcgctttctg attgttgtat
     3781 tagtggaaag gggaaaaaag gagcatattt atcatgcaac aaaattacaa tgagaaatct
     3841 atacaacatc aatttgatgc ctactgtaaa aaagtattac gaaacgaaac taagtctatt
     3901 cgaaaacgaa acattaacat aacagataat gaggaaccat tgacgaattc aaatgaaggt
     3961 aagtactccc aaactcattt tgatgaacaa gatgtctatt tcttatttgg aatggaaata
     4021 ttaatttcag atcaaaagtt atcggcagcc attgatcatt tatcggaaat aagaagaaag
     4081 gttatcctac tttattattt tgcaggtttt aatgatactg aaatcggtaa aatcctcaat
     4141 atgagtacaa gtggtatctg gtatcaacga aagaaggcag ttgaacaatt aaaaatgggg
     4201 tatgacttat ggtagacaac cccaaaaaac ggcagtatcc attagttcct ctatccatta
     4261 tccaatctgc tctagaagaa gatccaattt caatcaagat gatactcaaa cattatcata
     4321 gttatatagc taagttatgt ttgacaaatg gatttaatga ggcggggcaa tttattacct
     4381 atgtagatga gtatatgcta agacagctag aaattaaatt gatagaagcg atcttaaaat
     4441 ttaaaattaa ctaactaaaa agtccccttt ccacttattc tgtttaaaag aatcttctaa
     4501 tagttaaagg attagtagat tcttttaaac agaaaaccta ttgatcattg aaatttaaat
     4561 acattctatc agtacgttaa cacttcaagg agcatatttt agttgcgcca tgatttctta
     4621 ctgtaaagaa ggagcgataa acattctaaa ataattttta tagtcaataa aaagcgatga
     4681 tttgaagttg ggataatgat acttccgtaa ggctcgccgt aaacttggag agaggtgaaa
     4741 ttcctatgtg cttttgacaa aagcagctga ttaagaatta tttccatatg ttgatttcaa
     4801 cattttttct attattttta agaccaaatt cagtgaggca aatagcatca tgtaaattta
     4861 gtttttctat tgaacgttct ccacttttta gtttttttat agtactcaaa tttaatcctg
     4921 tatgtttaga gatctcattt gcagtatggt tttgtaaagt ttctcggatt attgttatat
     4981 tagcaatttc attacgcata attatgttcc tttcttatca aaaatatcaa tgactacagt
     5041 gggcttttag tgaaaaaaag gggtactaca gtctcttttg aaaacaaaaa aatacagtat
     5101 atacgttttc taaatgctaa gcaccaacgt ttacactgta tttaagggtt tggcacccaa
     5161 tgggctgtga ggggctcgaa cccgcgaccc gatgattaag agtcatctgc tctaccaact
     5221 gagctaacag cccaaattaa tactttgtta ttctaacacc taaaataaaa aagtgcaata
     5281 cttattatca gaattgagag tgaattttat ggattttcaa gattatgaaa aaatatttga
     5341 aagttatcca tcgatattga aaattactga tttaactaaa atgttaaata ttggaaaaaa
     5401 taaagcttat aatttgctga aagatagatc aatagaaaat ataaaaattg gagcaaggta
     5461 caaaatacct aaaaaagctg tcattgagta tatattgaaa atacaagatg aaggtaaaaa
     5521 tcatgctata ataaaatagt caaaattttt gccgatattt cagtttaaga ggagagattg
     5581 aattgaaatt aagagcaaac ataattgaaa aaagaggaac attacaagta gttgtatctt
     5641 ataaagactc taacggaaaa attagacaaa agtggaaaga tacaaaatta aaaaagaaag
     5701 gaaataaaaa acgagcagaa aaaattagag atgaattttt actagaattg gaaagtcaat
     5761 tagctatgga atgtgaatgt gctaatccaa atttattgtt ttatgactat ttgcttaatt
     5821 atttggaagt atcaaaaaaa caagtagcat ttaacacata tgttgggtat aagcattacg
     5881 tccataatcg aatttacaag tttttttatc ctaaaaaaat taaactaaat aggttaaagc
     5941 cttatcattt acaagagttt tatcaatata tgttaaatga taactgtaca gcaaatacgg
     6001 ttattcatta tcacgcattt atacgcaaag ctcttcaaga ggctgttatt acagaattga
     6061 taagtacaaa tgtagcagat aaagtaagaa agccaaaaaa gaaacaattt gtttctcaag
     6121 tttataatca acaagagatc ttgaaattac ttgacatttt gtcagaagaa aaactctatt
     6181 tggtagttct tttaacagca ttttatggat tgcgaagaag tgaagtttta ggattaaaat
     6241 ggtctgctat tgatttttta aacaaaaaaa tcatgataaa ccatgtaatc atagaaaatc
     6301 cagagaatat gtcacagtta ataaagaaag atcaaacaaa gaactcttcc agctatcgta
     6361 cattaccttt ggttgaaacg attgaacgag cattagtaaa tcaagctaaa tggcaacaag
     6421 ataatcgtat tcttttagga gatgattata ttttagaaga taatgagtac gtatttacta
     6481 tggaagatgg acggttgatg aagcctcaat atgtaacgca tcgtttgagt aagttgatta
     6541 agaaaaatgg attgaaaaaa attcgctttc atgacttgcg tcattctaat gcttcaataa
     6601 tgttagatag tggccagaat atgaaatcca ttcaagaatg gctgggacat gcatcgtatt
     6661 cgacaactgc taatttatac acgcatttaa ccgcaggagt aaaagatcgt gcagcagaag
     6721 cattagaaaa cgcatttggg tttaaagaat aaaaaaacaa aaaacaatat ctttggggta
     6781 tcgcttcttg tttttagaat ttttttgttg gcaatttgtt ggcagtatgt tggcaatgaa
     6841 aaaattcaca aactaaaaac ctatttataa agctttataa aacttttttc acaaatagag
     6901 catttggtta agagtcagct gctctaccaa ctgagctaac ggcccgtaca aaaagtactt
     6961 aataatagta gcactaagaa ttttaaaatg caagactaca tttaaagaaa atcagaaaaa
     7021 tctttacttc cttataggaa atcattcata gattattcac atttgctcgc tattatataa
     7081 gcataccaac aaacaaagtt cttgcaagag aacaaaccct aacacttttt tcatttttta
     7141 ctcctttccc gccgtctccc cggcgggtct cctgttctta acgataaaag acaagaaggc
     7201 ttagccttct tgttttttgt tttcatggaa tcccgctaat caaatcacct agttaaagca
     7261 actagattga atgtttatca taagtctgat atcatgttta tgaaatgaaa aaaggagcgg
     7321 ttatgatgga aaaaatgcgt cagcaattaa tggagtgggg gaaggaacta gaaacattcc
     7381 gtctgcctca ctgggaagaa ctccctgact tagaacttta tatggaccaa gtcagaacgt
     7441 tagttgatcg ttatctttca cctgtgatac aaggagaaaa gcatccattg cttacctctt
     7501 ctatggtaaa caattacgtt aaactaggtt tgataccagc accagttaaa aaaagatata
     7561 acaaagaaca cgtagcattt ttacttgcaa tcactacgtt gaaacaagta ttgactattc
     7621 ctgaaatcaa agaagggata ttgttccaag gaaaaacagt aggaattcgt gaagcctata
     7681 atctattttg cgatgaacaa gaagcagctg tgtggatggt cagccaatta gcacaaggta
     7741 aatctcatcc gcaaaaattc gaccaggcaa cccctgtaga atatattgct gtaagagcgg
     7801 caactttatc gtttgcaatg aaacttcttg cggaaaaaac gattgttcta gaaaccgaat
     7861 atttaaaaga ggagaaaaca aatgaaaaac aataaaatcg caattttagt agattcaggt
     7921 acggatgttc cagaagaatt gataaaaaaa tacgagatgt atatgatccc attgaaaatt
     7981 atttataaag atcaggtata tacagataaa attgacatta cccctgaaga gatctatcaa
     8041 cgtttgccag aggaaatccc tggtacatct ttgccggatg gtgagtcgat cacagaaatc
     8101 tttgagcaaa tcaaaaaaga cggttatgaa aaggtacttg cggtaacaat ttccagtgga
     8161 ttgagcggga catataatgt ggttcgttta attgcacaac agcaagaaga attggaagca
     8221 tatgtactag atacgaaaaa tattggtatt ggtgcaggtt tttctgcgat ccaagcagca
     8281 aaatggctgg aagaaggagt agaatgggat caactgatca gtaatctgaa tgagttagtc
     8341 gaaagaacaa aagttttctt taatgttgcg acattggaat atcttcaaaa aggtggacgg
     8401 atcggtttag ttgcttctat tgtaggaacc gctttaaagc ttaacccaat catctcatgt
     8461 aatgaagaag ggatttatta cacagtagga aaagctaggg gacgtaaaaa aagtttggat
     8521 aaaacggttg cttacgtcaa agaaaaaatc ggtaatgcaa aggtcttcaa cttagctgtt
     8581 gcccatgggg atgcgaaaga agaagcaatt gaaatggaag cgagacttaa aagagagttc
     8641 ccacaagcaa accaaatcta ctttggccag atttctccgg cattagtagt ccacactggt
     8701 ccgggattat tgggtgtcgg tgtacaagtt ttagaaggat aaatcaaaaa aaggaaacac
     8761 tgaggtgaaa atcagtgttt tcctttttta ttgaagttag aacaaaaaat aataaagtat
     8821 tttgcacttt cattttaatt ttctcttttg ttttataggc aaagatgaaa caaaagcaga
     8881 aatacggtat aatgagaagg tatgacaata atatataaga ggtggctctt tatgaaaaaa
     8941 gttttagttg tcgatgatga gaagccgatt tcggatatcg tcaaattcaa tttagccaaa
     9001 gaaggctatg acgtttatac tgcatatgat ggggaagaag ccttggaaaa agttgctgag
     9061 gtagaacctg atttgatttt acttgatttg atgctaccta aaatggacgg cttagaagtt
     9121 gctcgtgagg tacgcaaaac ttatgatatg ccgatcatta tggtaacagc caaagattct
     9181 gaaattgata aagtcctagg cttagagtta ggcgccgatg attacgtgac caagccattt
     9241 tccaaccgag aattagttgc acgtgtcaaa gcaaatcttc gtcgcggtgc cacggctgcg
     9301 aaggaaccag aagaggcagc acctgcggaa ttgacgattg gtgatttgac gatccatcca
     9361 gaagcttaca tggtgacgaa acgaggagaa acaatcgagt taacacatcg tgaattcgaa
     9421 ctactctttt acttggcgaa gcaccttgga caagtgatga caagagaaca tttattacaa
     9481 acggtttggg gttatgatta ttttggagat gtacgaacag tggatgtaac tgtacgtcgt
     9541 ttgcgtgaaa agatcgaaga taatccaagc catccaaatt atttagtgac acgtcgtggt
     9601 gtcggttact atttgcgtaa cccagaacag gagtaattgg atgaaaagaa aagtccgctt
     9661 ttttcgatca gtcaatttta agatcgctat tacctttatt ctgattttat tgatctcgat
     9721 cgaaattatt ggtgcctact ttatacgtgg tttagaacgt tccacgatca atacatttat
     9781 taaagatatg aatcaaacgg tggaatcatt agcaacaaca atcagcccgg aattgaatcg
     9841 aaaagataat gcagatgatg aagaggtaaa tgcgaatatc aaaagattta ttgaaaacag
     9901 tgcgactagc gatatcatcg aaatacgggt cgttgacgaa aaagggatca tccgaggaac
     9961 aaccgatgtc aatgaacaaa gcgctgtggg aaagaaaaat gattatgttg atatcaatga
    10021 tttcacgact aaacgttatg ttgctttaga taacgacgat aaacgggtga atatcaatgt
    10081 tcaaccgatc ctgtccccga ccggcgatgc cgttatcggg gctctttacg tcaaaagtaa
    10141 tatcgaacaa aaatattcgg aaatcaataa cacagcagtc attttcttta ctgcctcact
    10201 aattgcggca tttatttcaa tgatcgtatc tgttttagtt gctcgttcga ttacacagcc
    10261 gatcggtgaa atgagagaac aagccattcg aatcgctaga ggagactaca gtcggaaagt
    10321 caaagtgcat ggacaagatg aacttggaca gctggctgat accttcaatc agttagctga
    10381 acgtattgaa gaagctcaag atacaatgga agctgaacgc aatcgtttgg atagcgtgct
    10441 atcccatatg acagacggcg tcattgcgac agatcgcagg ggtaaagtca ttacgatcaa
    10501 tgatatggct gtttccttgc tagatatcaa aaacgaggaa gcaatcggac aatcgatttt
    10561 gacactttta gatatcgaag aagaatatac gttgcggaaa ttattagaag atccaaacga
    10621 aatggtactt gaccgttcaa ccagtacatt ggaaagtgat cagatgatct tgcgagtaga
    10681 cttttctatg atccgtcgcg aatcaggttt tatcagcgga ttagtagctg tcttgcatga
    10741 tgtgacagaa caagaaaaaa cagaacggga acgtcgtgaa ttcgtctcta atgtttccca
    10801 tgaacttcgt acaccgttga caagtatgcg tagttatatc gaagcattga gcgaaggtgc
    10861 gtggaaagac gaggaagtag caccaaattt tttgaaggta acgctagatg aaacagaccg
    10921 aatgattcga atgatcaatg acctcttaaa tctatctcgc atggatacag gtaataccca
    10981 gcttcaatta gaatatgtca atttcaacga gttagtgaat tttgttcttg accggtttga
    11041 tatgatggtg ggaaatcagg aaaaaaatta taagatccgt cgtgaattta cgcaaagaga
    11101 tctttgggtc gaagtagata cagataaaat catccaagta gtcgataata tcatgaataa
    11161 cgcgatcaag tattcaccag atgggggaac gattacttgt cgcttattag aaacgcataa
    11221 taacgtgatt ttaagtatca ccgatcaagg attggggatt cctaaaaaag atctgaaccg
    11281 agtgttcgag cggttctatc gagtagataa agcacgtgca agagcacaag gtgggacagg
    11341 acttggatta gctatttcta gagaagtgat taaggcgcat cgaggcgcta tttgggcaga
    11401 gagcaaggaa ggaaaagggt cgacttttta tatctcgctg ccatatgaac cttatgagga
    11461 ggaatggtgg gaatgagaat ctctgaaaaa gtcgttcgta taggactcat tttgatggtt
    11521 gcgttaagca tatacttctc ttatgcaatc tggctgagcc cagcaggaaa aagttcaatc
    11581 aacgtagatg agacaaacac tcaaatgatc gagtctcaaa gcaatcgaaa aacttccgaa
    11641 gtctttcttc cgcttcatgt tacatggcac cattcgggaa tgatccaaga aaccaacagt
    11701 gaaaatctcg tgtaccgatt gcaattgatc atcgataaag caagatttgg aaagctttct
    11761 gaagtagttt caggagacaa atcacagttt gaacaggtga aaaatctgaa ctctggtttt
    11821 gagttggcct ataatgcgcc ttttttgcta agagaataca aggaagcatt tgatttagat
    11881 ttagatgtgt cgaacttggc aaaagaaagc gaaaatatgt attttactag aatacaattc
    11941 gatcaaggaa ataaaaaaat ccgatttttg aattattcga accatctcgt atatgaagca
    12001 aatatttcgt tgaattggac agatatcaat aaagaattaa cagcaaaaga cacaaaatgg
    12061 acagaaatga caacagaacc atcgacgatc gacacgcaat atgctacgaa acattcgatc
    12121 aaactgaaga agtatagtta tatcctgtca cagcaaccct atacactttt tagaaatgct
    12181 tttttccaga aaccagatag cgtgaaaaat aatgatgaaa gcaatgaatt gattttttac
    12241 ggtgggaatg aaacaatgtc tatccattcg gaaacgcaag tagttgattt tcgtggagag
    12301 ctgccagaga aaaaagaaaa tgctagtgta ttttctgaaa gtttccccta tattagcaaa
    12361 ttaggcagtt ctttagggaa cctcagatat tttgaccgca gtgacaatac gatcgattat
    12421 cggatattcg tggaaggctt cccggttttt gggacagaca gcagaggaca gattggtgtg
    12481 gaagttggcg gtgaaacgaa tggttctgtg caagtgaata tccaaaccag tttgaatacg
    12541 attcaagtgc cgataccttc agacgaagaa gtagaacttg caagtacgaa tgaaatcatc
    12601 cagcagttaa aagctaaggg agcagatagc aacaagattg gttcagtgat cgttggctat
    12661 acatggcaaa atgtcaaaga aacgaatcgt gtcgttgatc tcttacctga atggtatgtg
    12721 aaatataaag ataactggta ttcagctaac gaccttctgg aaaaattacc tggattggag
    12781 gcgaattaat tggatttcaa aaaaatcgaa tggatatttt tcgtcgcttt tttagggttg
    12841 aatgtgtttt tattcagcag ttatcatgac gcggtatacc aagaaaacaa tgtcattcgc
    12901 tctaatcaga ctgaatcaat ccaacaacgt ctggctagcg acgatatcac ttacacgggg
    12961 aagatttcgt ctgagaacaa gcaaggctat tatcttagcg cggagcagac caatcttagc
    13021 acttcattag cgaattatcg caaggctggt aaaagcggct tgctggcaag tggtgtgttt
    13081 ttagacggtg atgttttgac gaaatcttta tccagttcag atgctgaaaa gaacgtgatc
    13141 gatccagata aaattagtag ttccttggaa gattttttat ctaacaaaga caatatttta
    13201 ttcggtaatg attatgtgta cacgaaaaac ttttctcatt tagaagaaga accactagaa
    13261 atccaagcaa gccaagagta tgaaggcatc ccattcaatg atgatacagc aaaaatcgtt
    13321 ctttctttag aaaaaaacga taatgattat agcatcacga aatacatgca gacgcattta
    13381 tctgatatcg agcagctgcg cgaaaagacg gaactgtata cggaagagga agcaatcaat
    13441 acattgtaca tcaataacaa aataccaaga ggatcaaaaa ttttatggcg tcagctagct
    13501 tattcctgta ttctgaaagt ccgggaaaaa aatgtgtatg ttcctgtttg gcatgtagcg
    13561 atcgaaacaa gcgataaagt ggtccaaatt gagagcgtca acgctttcag caatacgatc
    13621 gtgacaaaca atactgtacc aaaagtggaa gatcaataaa aaagcgagta taatgaaaaa
    13681 agtttacaaa gtaagaaggg aagtaggagt caaatggctg aagaaaaacc agcttttaaa
    13741 atcagtatcc tagcaagtgg aagttcgggt aattcacttt atatcgaaag tgaaaaaaaa
    13801 cggcttttgg tcgatgcagg acttagcggt aaaaaaatca cttccttgat ggctcaaatt
    13861 gatcggacac cggaccaatt agatggaata cttgtgacgc atgaacatcg agatcatata
    13921 catggcgtgg gtgtcttggc tcgtaagtat catttggatg tttttgccaa tcagcagact
    13981 tgggaagcga tggaaccact gattggaaat gtaccagtgg aacaaaaaca tctatttgag
    14041 atggggaaag tcagaacatt cggcgatatc gatattgaga gtttcggcgt ctcccatgac
    14101 gcagcggctc cgcaatttta tcgtttccat aaagaaaatc ggtcattcgt catgttgacg
    14161 gacaccggat attgcagtga ttatatgcga ggtgtcattg aaaatgccga tggttatttg
    14221 atggaaagta atcatgatct tgagatgtta cgcatgggac cttatccgtg gaatttaaaa
    14281 cagcgtattt taggagatag aggacatctt tccaacgaag acggtgcact tgtcatgact
    14341 gatgtgatcg gtaatcggac caaacgaatc tatcttggcc atcttagcaa ggaaaacaat
    14401 atgaaagaac ttgctcattt aacaatggaa aatgttttga aagaaaaaga ttttggcgtt
    14461 ggacatgatt ttgaaatcta tgatacagat ccagatgaag caactgagct attttcgatt
    14521 taaacagttc aatcaataaa aatataaaaa gcagaagata aaatcaatca gggt
//
LOCUS       DARBFD010000065        14504 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000065 DARBFD010000000
VERSION     DARBFD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.065"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(308..670)
                     /locus_tag="SCK08_001940"
     CDS             complement(308..670)
                     /locus_tag="SCK08_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201805.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(744..1124)
                     /locus_tag="SCK08_001941"
     CDS             complement(744..1124)
                     /locus_tag="SCK08_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEG1201806.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1188..2381
                     /locus_tag="SCK08_001942"
     CDS             1188..2381
                     /locus_tag="SCK08_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1201807.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2458..3042)
                     /locus_tag="SCK08_001943"
     CDS             complement(2458..3042)
                     /locus_tag="SCK08_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1201808.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3243..4241
                     /locus_tag="SCK08_001944"
     CDS             3243..4241
                     /locus_tag="SCK08_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1201809.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4353..4862
                     /locus_tag="SCK08_001945"
     CDS             4353..4862
                     /locus_tag="SCK08_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEG1201810.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4892..5551)
                     /locus_tag="SCK08_001946"
     CDS             complement(4892..5551)
                     /locus_tag="SCK08_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEG1201811.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5553..6317)
                     /locus_tag="SCK08_001947"
     CDS             complement(5553..6317)
                     /locus_tag="SCK08_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEG1201812.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6325..7002)
                     /locus_tag="SCK08_001948"
     CDS             complement(6325..7002)
                     /locus_tag="SCK08_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEG1201813.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7039..8424)
                     /locus_tag="SCK08_001949"
     CDS             complement(7039..8424)
                     /locus_tag="SCK08_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1201814.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8634..8718)
                     /gene="ffs"
                     /locus_tag="SCK08_001950"
     ncRNA           complement(8634..8718)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="SCK08_001950"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="SCK08_001951"
     CDS             complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="SCK08_001951"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEG1201815.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9363..10220
                     /locus_tag="SCK08_001952"
     CDS             9363..10220
                     /locus_tag="SCK08_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201816.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10301..10636)
                     /locus_tag="SCK08_001953"
     CDS             complement(10301..10636)
                     /locus_tag="SCK08_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201817.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10703..12073)
                     /locus_tag="SCK08_001954"
     CDS             complement(10703..12073)
                     /locus_tag="SCK08_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEG1201818.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12151..12687)
                     /locus_tag="SCK08_001955"
     CDS             complement(12151..12687)
                     /locus_tag="SCK08_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1201819.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12996..14477
                     /locus_tag="SCK08_001956"
     CDS             12996..14477
                     /locus_tag="SCK08_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201820.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4470 a         2887 c         2355 g         4792 t
ORIGIN      
        1 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
       61 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      121 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      181 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      241 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      301 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      361 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      421 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      481 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      541 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      601 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      661 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      721 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
      781 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
      841 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
      901 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
      961 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1021 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1081 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1141 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1201 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg
     1261 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1321 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1381 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1441 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1501 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1561 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1621 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1681 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1741 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     1801 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     1861 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     1921 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     1981 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2041 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2101 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2161 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2221 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2281 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2341 gacttaatcg aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa
     2401 aggacgagac cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta
     2461 ttctgaaatt tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg
     2521 ttgtacattt gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt
     2581 atgtcggaaa tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg
     2641 gaatccagca ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat
     2701 aaagaattgg cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag
     2761 gttgaacagt tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc
     2821 cattcctgtt ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa
     2881 aatcacgcca tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg
     2941 tgctagttca gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt
     3001 tggtcctttt tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat
     3061 ttgcatactt cataataata ataacataat acctaacctc tcgtaaaaat actagatttg
     3121 ttttctttta tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc
     3181 aaacattttt gagaagcatt ctcaattaaa ttctgctata gtgtgaaaag ggaggaattt
     3241 ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt
     3301 tttacgcagg aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg
     3361 gtcaattagc aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa
     3421 taaaagcaag tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat
     3481 tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta
     3541 caaataaagg catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc
     3601 agccaagaaa attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt
     3661 tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt
     3721 ctgctatcga ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc
     3781 gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca
     3841 acttgcttac tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata
     3901 tccggttaaa aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg
     3961 tcaattatgg attctcttct aatttagaac atctttcttc ttggggactt gatagtacca
     4021 gaaatgccat tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg
     4081 gtgacatctg tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac
     4141 ctactgcagt gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc
     4201 acagcacaag cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca
     4261 tttcaagtta aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat
     4321 agagaaagaa aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag
     4381 aaaaggcaag agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg
     4441 tcatgttttt acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg
     4501 tcaacagcgt gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag
     4561 atatattagc agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg
     4621 aaggccttta cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga
     4681 tcggctttac gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa
     4741 acagccacga tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg
     4801 ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat
     4861 aattcaacaa aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt
     4921 tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa
     4981 ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa
     5041 cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc
     5101 gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa
     5161 acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac
     5221 tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat
     5281 aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga
     5341 agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta
     5401 aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt
     5461 tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat
     5521 attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt
     5581 ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat
     5641 aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta
     5701 tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata
     5761 atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa
     5821 gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg
     5881 ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca
     5941 taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta
     6001 aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc
     6061 ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct
     6121 ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta
     6181 gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat
     6241 cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa
     6301 tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt
     6361 tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga
     6421 ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt
     6481 ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc
     6541 ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta
     6601 aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg
     6661 gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg
     6721 ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc
     6781 ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac
     6841 tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt
     6901 cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt
     6961 tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt
     7021 ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac
     7081 acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa
     7141 aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat
     7201 tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac
     7261 catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa
     7321 gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac
     7381 atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc
     7441 ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg
     7501 ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact
     7561 cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag
     7621 ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc
     7681 tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac
     7741 atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct
     7801 gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat
     7861 ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg
     7921 taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg
     7981 attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt
     8041 cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa
     8101 taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt
     8161 ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat
     8221 cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa
     8281 atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg
     8341 ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt
     8401 atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat
     8461 aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca
     8521 aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt
     8581 ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat
     8641 cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag
     8701 cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc
     8761 agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca
     8821 tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt
     8881 cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat
     8941 aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg
     9001 taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga
     9061 ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc
     9121 ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt
     9181 ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa
     9241 gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata
     9301 tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca
     9361 agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag
     9421 catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag
     9481 attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc
     9541 aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt caagaaaaaa
     9601 gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc
     9661 aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag
     9721 atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta
     9781 tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc
     9841 attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag
     9901 agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt
     9961 actatacaaa agatactgta aaagatagta agttgatcaa aagttacata gaactgatca
    10021 aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct
    10081 ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc
    10141 aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat
    10201 tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac
    10261 cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa
    10321 aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag
    10381 agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac
    10441 tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga
    10501 cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg
    10561 atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt
    10621 ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa
    10681 aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta
    10741 ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc
    10801 atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt
    10861 gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc
    10921 taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta
    10981 aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa
    11041 gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct
    11101 cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt
    11161 gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata
    11221 tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg
    11281 attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga
    11341 tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt
    11401 ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag
    11461 attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac
    11521 aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg
    11581 gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa
    11641 tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag
    11701 cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg
    11761 gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc
    11821 cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg
    11881 gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta
    11941 gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg
    12001 atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg
    12061 cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa
    12121 ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc
    12181 caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc
    12241 ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga
    12301 cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag
    12361 cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa
    12421 aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta
    12481 gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt
    12541 gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt
    12601 aattgacttc attccatatt cttccacgta taccatactt ttgaacagca aatattcttt
    12661 actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc tacccataaa
    12721 taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata tttagttata
    12781 tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa ataccctagg
    12841 aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata taaaaaagaa
    12901 ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag tttttgtcta
    12961 actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca agaaaaaaag
    13021 agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa gaattatcca
    13081 atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata aatgtatcac
    13141 tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga gagatccaaa
    13201 ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa ttatgctacc
    13261 taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca caaactgctg
    13321 taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc gaaaaactga
    13381 caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct gtaaaaaatt
    13441 gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt tttctttcag
    13501 atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca ctaaaaatga
    13561 acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc tacttgctgt
    13621 taactttatt catattacga aacaaacaac aggcatattt tccaaaaact tcttctgaaa
    13681 tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac tttacttctt
    13741 tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt aattattttt
    13801 tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt ggttttttat
    13861 tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact tacttaactc
    13921 aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat tttcttgtgt
    13981 tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct acactttgct
    14041 ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa ttaagagaaa
    14101 aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa taccttagtc
    14161 aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc catttgcaga
    14221 taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag ttatttcgca
    14281 aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt acagatcagc
    14341 atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac aatacagtgt
    14401 tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt tatgagaaca
    14461 aaacaaagag gatgtgacaa aattcttgtc acatcctctt attc
//
LOCUS       DARBFD010000066        14342 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000066 DARBFD010000000
VERSION     DARBFD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.066"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            108..489
                     /locus_tag="SCK08_001957"
                     /pseudo
     CDS             108..489
                     /locus_tag="SCK08_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            620..1570
                     /locus_tag="SCK08_001958"
     CDS             620..1570
                     /locus_tag="SCK08_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1201821.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2088..2543
                     /locus_tag="SCK08_001959"
     CDS             2088..2543
                     /locus_tag="SCK08_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1201822.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2884..3486
                     /locus_tag="SCK08_001960"
     CDS             2884..3486
                     /locus_tag="SCK08_001960"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201823.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3580..3912
                     /locus_tag="SCK08_001961"
     CDS             3580..3912
                     /locus_tag="SCK08_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HEG1201824.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(4063..5042)
                     /locus_tag="SCK08_001962"
                     /pseudo
     CDS             complement(4063..5042)
                     /locus_tag="SCK08_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5047..5814)
                     /locus_tag="SCK08_001963"
     CDS             complement(5047..5814)
                     /locus_tag="SCK08_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1201825.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6304..6729
                     /gene="lacA"
                     /locus_tag="SCK08_001964"
     CDS             6304..6729
                     /gene="lacA"
                     /locus_tag="SCK08_001964"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEG1201826.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6746..7261
                     /gene="lacB"
                     /locus_tag="SCK08_001965"
     CDS             6746..7261
                     /gene="lacB"
                     /locus_tag="SCK08_001965"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEG1201827.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7272..8204
                     /gene="lacC"
                     /locus_tag="SCK08_001966"
     CDS             7272..8204
                     /gene="lacC"
                     /locus_tag="SCK08_001966"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEG1201828.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8207..9187
                     /gene="lacD"
                     /locus_tag="SCK08_001967"
     CDS             8207..9187
                     /gene="lacD"
                     /locus_tag="SCK08_001967"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1201829.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9215..9532
                     /locus_tag="SCK08_001968"
     CDS             9215..9532
                     /locus_tag="SCK08_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1201830.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9532..11238
                     /locus_tag="SCK08_001969"
     CDS             9532..11238
                     /locus_tag="SCK08_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1201831.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11380..12786
                     /gene="lacG"
                     /locus_tag="SCK08_001970"
     CDS             11380..12786
                     /gene="lacG"
                     /locus_tag="SCK08_001970"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG1201832.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12809..13081
                     /locus_tag="SCK08_001971"
     CDS             12809..13081
                     /locus_tag="SCK08_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEG1201833.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13102..14001
                     /locus_tag="SCK08_001972"
     CDS             13102..14001
                     /locus_tag="SCK08_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG1201834.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4685 a         2363 c         2765 g         4529 t
ORIGIN      
        1 gacactatcg ttcttcatca acgtaagtta gatttgttga aagagcagaa aaaaggattt
       61 ctacaaaaaa tgtttgtttg atattttaaa tagaaaagag agataaagtg aagtttttaa
      121 attttttgaa ctataaatat ttaaactggg tattatatgg tattacatta ttttttatta
      181 tttattactt ttttaccgat gatgatattt ttttaatttt agcaatactt tttcttgttt
      241 catctaattt tagtgataac ttaattgaag ttcttacaga accagaaatt cggtctatga
      301 cggatgaaga acaacagatt attaaagcta tgatttcgga aaataagaaa gacgtagtag
      361 ttatcaaaaa gattagagag tatacaaatt taggtgtttt acaagccaaa aaagtatatg
      421 aaaaaaaaaa aaagggtcta taattacaaa ttagataata cattagaagc tggagacaag
      481 attgtctaga taatgtgtga gataaaattt ttggcaggtt atcatacata agacaaaatg
      541 tttttaaaac tgataacgta tttattccac aacaattacg catttatttc tgagaaaaat
      601 ataaagcagg aggattacaa tgctgaaaac ttattcctta acaaaagata cattattagt
      661 ttcgtcagaa tcaaatatca aaaattcttc tgttgtttat gtttatggtg acgaacgaga
      721 atatatcgaa tcttctgaac ataaatatga ctttaaaata ggcaatatac taacgtttga
      781 tgatcgagtt gcatatgaca cgatgataga taagaacgat gaaaaaagtt tgctcgtaag
      841 ttttttgttt cccgaggtac atgaaggtgg gcatctagat accctgacga catctgttgt
      901 tttcttggta tttaaaaata agttgataat ttttacaaaa caaaaattaa aattcattcc
      961 cgaactctta tccaatatgg ttttacgtga tgtcaatcat ttcgtggagg aagtactttt
     1021 aaagatcatg caaaaagatt tctatatgat gcttaacgac cttcgtggag tcaaggaaaa
     1081 aattgatgat ttagactcag aaatatcaac gtccggttca ctcaaaccaa cgtttggtga
     1141 tttattgact cttcaaaaat acatgatagc tttgtcaaca acttataaag ctaaccataa
     1201 agcgcttgat ttcattaaaa aaaatttcac aactattgac gatattgatt ttagtactaa
     1261 aaaaataatt gatgatttat acgatacaag tgatacaatg gatagaatca ttttaagtta
     1321 cagccaatat ttggacaaat tggaaaatat gataaacaat atgatttcgt atcagctaaa
     1381 catgattatg aaaaccctaa ccgagatatc aattgttttg actattccag caattatttt
     1441 tggcttttgg ggaatcaatg tccatgtccc gtttgaaaag tcttcttatg gtgttttagc
     1501 cgtattgatt atctctgcta tactaagtct aatttgctgg cttttgatgc gacgcaaaac
     1561 atatttgtaa agtatttgtg ataccctaat atgatttaaa gcagaatttc tttgttgact
     1621 ttttaaaagg ctgattcctt tcaataattt aatagcttca gcggctgctc agaagtcaat
     1681 aaattggact atttttattc agttacttag gcaacatttg agatacgatt gcgattataa
     1741 aaattgatat accaaccaat ggctgcttgg aagactgaca tattagcctc gatatcatcg
     1801 aataactaaa tacagaaaaa tacaataaat attatcaaac tagggactat actagaaaac
     1861 atcagtacat tccttaactc agggtctata attagaaaat cgcccctttg aaaactgatt
     1921 aaaacagccc ccattatcta gtgactagat aatgggggct gtttttttgt taagtgatat
     1981 aataaaccat aaaagaagtt tagaaatacg gtcctggtca tcaataaata aacaatttaa
     2041 ttaattattt atttaattat ttaatttcag aaaggtatac atgaatcatg gtgcaacaaa
     2101 ttgtcctacc catcaaagac tccagcgtct taaagatggt acaagataca cttctcgata
     2161 gtttccgtgc tggtcgccga aattacaccg tttttcaagt tggtaaggcc acgctactac
     2221 gtgtgagtga tgtcatgaca ttaaaaaaat cagatgtcta taatccagac ggctctgtca
     2281 aaaatgcaac ctttattcat gataaaaaga ccggtaaagc aaacacgtta tatttaaagc
     2341 ctgttcagca agacttgttg caatatcatg attggctggc ccaagaaaat atcaattctg
     2401 agtggttatt tccctcgaca gcccattatg atcttcatat cactgagaag cagtttcata
     2461 aggtgatggc gcatgttggt gatctcttag gaatcaactt ggaagacaaa gagaaaaaat
     2521 tgcgagtcag aaaaccgttt tgagatatga acagcatttg ccttcacgcc ttagcgattt
     2581 taaataatag ccttgaaagt gattatggaa aagccctcta cgctcaatgc aaaattgacg
     2641 tagaaactat ttttgatcga ttgaaaggtg tatttggtat gtgaagaacc catgtcagag
     2701 gcaagaacgt tgcacctact gacattggaa tgatgcttat gagcatgaat ctaaccaaat
     2761 tatcccttga ggctagaaga aaagcggaag cttttcaaca caaagcagcc aaaaataaaa
     2821 aacgcgacga agcaatcaca tttatgatta ttcatcgcgt tttttatatt tagagctagt
     2881 tatttgctag acccactatt tacagatgaa acgtttatca cctttggttt tgggcattta
     2941 gtggaattag cagaaccagg tcattatgac gaaaagtggc aaaattggaa acttgaatct
     3001 ttgccgattt ttcctgatcg atacgatttt gaagttgcaa aagataaggg aaagcagttt
     3061 aaaattgttg cagaacttct caaaaaggca aatacaatta ttgttgcaac agatagcgac
     3121 agagaaggtg aaaatatcgc ctggtcgatt atccataatg caaatgcctt ttcaaaagat
     3181 aaaacattta aaagactatg gatcaatagc ttagaaaaag atgtgatccg aagcggtttt
     3241 caaaatttgc aaccaggaat gaattactat cccttttatc aagaagcgca aacacgccaa
     3301 attgccgatt ggttgatcgg catgaacgca agccctttgt atacgttaaa tttacaacag
     3361 aagggcgtac aaggtacatt ttcactagga cgtgttcaaa cgcccacctt ataccttatt
     3421 tttcagcgcc aggaagccat agagaatttt aaaaaagaac cttttttcga ggtggaagct
     3481 aagtaaaaca agggtcattt aagggcgttc taagccccac acagcgtttt aaaacccaag
     3541 aggagctttt tagcttttgt ttcttctaaa caagctaaaa taggcaatca agaggggata
     3601 attgctgatg ttcaaaccaa agagaagaaa acgaatagtc cgagttcgtt ttctttaagt
     3661 agtttgcaat caaaagtcaa tcagctttat aaagcgacag cgagccaaac tttaaaagct
     3721 atgcaaggac tgtatgaagc aaaattattg agttatccaa gaacagatac accatttatt
     3781 acagagaacg aatttgctta tttaaaagcg aattttggca aatatagcgg ttttttagga
     3841 cttgatcttg aaatgttgtt atcattgata atgatacgtt caaacaacag cacccaaatt
     3901 ttgacgaatt agtgaaactc tatgaaaaag acgtagtaaa acatgcaaca tcctattcta
     3961 atcagctggt aaagcttaat taggttatat aagcttgaaa ataaagggtt tgtccaatat
     4021 ggacaagcct ttttcttatg ttttgccaca gatttgccac acttattgtg taagagtatc
     4081 aaggataggt ataatttgat actttgtttt tttagtgatg tgcaaataaa tttctgttac
     4141 acgactacct ctagaatgcc ctacacggtc ttgaattgct tctaggggta ttccttactc
     4201 tgctaagaaa ctaatatgcg tatgacggaa tatatgggtc gtaatatgtt tattaaaact
     4261 aagtttatta tcaatatcaa ttcctgctcg ttttggatta ccttttcgat ttaattttcc
     4321 attttcaggt gagttgagga aattgttaag gacactataa tgaagaggtg ttccattata
     4381 gtcgggttta tgacgacttt tggaagtaaa aatccatcca ttatctttat aatcaggatt
     4441 gaatctcata tgttggtgat ttaactcaat ttcctcttta agaatttcta tagccctttt
     4501 agataattgg acatttcggt aactttgtaa gttttttaca ttaccttcat agctattttc
     4561 tgcagctcct ccgtgagtgg actgactaat gatgatattt tgcttttcaa aatattgata
     4621 ggcaccaata ttttggagtt tcgactctgt aagtcaacag ggccattagt tagaatacct
     4681 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
     4741 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
     4801 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
     4861 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaatatct gaaacgtttg
     4921 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
     4981 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
     5041 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
     5101 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
     5161 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
     5221 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
     5281 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     5341 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     5401 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     5461 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc caggtccaat
     5521 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     5581 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     5641 caattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     5701 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     5761 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     5821 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     5881 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     5941 ataagaacag aggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     6001 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     6061 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     6121 gttatctatt ttttaaaaaa acaaataaaa aaaacaaaaa ataaacaaaa aaagtgttgc
     6181 tttttgtttg aatgcttgat attatataaa caaagaaatg atgaaaacgt tttttcgaac
     6241 attttgcaaa atgttttcta cttctacgta gcatttcttt ctaaaattta ggaggtggtc
     6301 aaaatggcta ttgttgttgg tgcagatctc aaaggtacga gattaaaaga tgttgtgaaa
     6361 aattttcttg tagaagaagg ttttgaggta attgatgtga ctaaggacgg acaagatttt
     6421 gttgatgtta ccttggcagt tgcctctgaa gtaaataaag atgagcaaaa cttaggtatt
     6481 gtaattgatg cttatggagc tggtccattc atggtagcaa ctaaaattaa aggtatggtt
     6541 gcagcagaag tttcagatga acgctcagct tatatgacac gtggacataa caattcacga
     6601 atgattacgg ttggcgctga aatcgtcggt gatgagcttg ctaaaaacat cgctaaaggt
     6661 ttcgtcaatg gtaaatatga tggcggacgt catcaaatcc gagtagatat gcttaacaaa
     6721 atgtgttaag aaaaggagtt aaggaatgag aatcgcaatt ggatgtgacc acattgtcac
     6781 agatgaaaaa atggcagtat cagaattctt gaaatcaaaa ggacatgagg tccttgattt
     6841 cgggacatat gaccacgcac gtactcacta ccctatctat ggtaaaaaag tcggtgaagc
     6901 agtagtaagt ggtcaagcag acttgggagt atgtatctgt ggtacaggtg tcggcattaa
     6961 caatgcagtc aataaagttc caggtgttcg ctcagcgttg gttcgtgata tgacatcagc
     7021 tatatatgca aaagaagagt taaatgcgaa tgttatcggt ttcggtggaa agattactgg
     7081 tggtcttctt atgaatgaca tcattgaagc tttcattgaa gctgagtaca aaccaacaga
     7141 agaaaataaa aaattgattg caaaaatcga acacgttgaa acacataatg cacatcaagc
     7201 agatgaagat ttctttacag aattccttga aagatgggac cgtggtgact accacgatta
     7261 aggtataaca aatgattctg acagtcacac taaatccttc agtagacatc tcttatcctc
     7321 tagaaacatt gaaaattgat acagtgaata gggtgaaaga tgttagtaag acagctggtg
     7381 gaaaaggctt aaatgttact agagtcttgt atgaatctgg tgataaagtt actgctacag
     7441 gtttcttagg tggtaaaatt ggtgaattta ttgaaagcga attagaacaa tctcctgtta
     7501 gtccagcttt ttataaaatc tcaggaaaca ctagaaactg tattgctatc ttacacgagg
     7561 gtaaccaaac agaaattttg gaacaaggac caacaatttc tcatgaagaa gcagaaggct
     7621 ttcttgacca ctatagcaat cttattaaac aatcagaagt tgtgacaatt tcaggaagtt
     7681 taccttcagg acttcccaac gattattatg aaaaacttat ccaacttgct tcggatgaag
     7741 gagtagcagt cgttttggat tgttcaggtg cacctcttga aacagttcta aaatcgtcgg
     7801 ctaaacctac tgccatcaaa cctaataatg aggaactttc acagttgctt ggaaaagaag
     7861 taacaaaaga tattgaagaa ctcaaagacg ttcttaaaga gtcacttttt agtggtatcg
     7921 aatggatagt ggtctcatta ggtcgaaacg gtgcctttgc aaaacatggt gatgttttct
     7981 ataaggtaga tattcctgat atcccagttg ttaatccagt cggatcaggg gactcaacgg
     8041 tagctggtat tgcatcagct ttaaatagta aaaaaagtga cgctgactta ttaaaacatg
     8101 cgatgacatt gggtatgtta aatgctcaag aaacaatgac agggcatgtg aatatgacta
     8161 attacgaaac actaaactca caaattggag taaaagaggt ataaaaatgg tacttacaga
     8221 acagaaacgc aaatctttgg aaaaaatttc agataaaaac ggtgttatct cagctttggc
     8281 atttgaccaa cgtggtgctt tgaaacgttt gatggcacag tatcaagata cagaaccaac
     8341 tgtagctcaa atggaagaac taaaggtttt ggttgctgat gaacttacaa aatacgcttc
     8401 ttcaatgttg cttgaccctg aatatggact tccagcaaca aaagcattgg ataaagaagc
     8461 aggtcttctt cttgcctatg aaaaaacagg ttatgataca tcaagtacaa aacgtttgcc
     8521 tgactgcttg gatgtttggt ctgcaaaacg tatcaaagag caaggcgctg atgccgttaa
     8581 gttcttgctt tactatgatg tagacagctc agatgaactt aaccaacaaa aacaagctta
     8641 tattgaacgt gttggttctg aatgtgtcgc tgaagatatt cctttcttct tggaaatcct
     8701 tgcttatgat gaagaaatct cagatgcagg ttctgttgaa tatgcaaaag tgaaacctcg
     8761 caaagtcatt gaagcgatga aagttttctc tgatccacgc tttaacattg atgttcttaa
     8821 agtagaagta ccagttaacg ttaaatatgt cgaaggtttt gctgatggtg aagtagttta
     8881 cagtaaagct gaagctgctg acttctttaa agcacaagaa gaagcgacta accttccata
     8941 catctaccta agtgcaggtg tatctgctaa attgttccaa gaaacattgc aatttgctca
     9001 cgactcaggt gccaagttta atggtgtgct ttgtggacgt gctacttggg caggatctgt
     9061 agaaccttac atcaaagaag gtgaaaaagc agcacgtgaa tggttgcgta ctactggatt
     9121 cgaaaatatt gatgaactta acaaagttct tgttaaaaca gctagtccat ggactgataa
     9181 agtatagtaa aaacataaaa cggaggatat tgttatgaac agagaagaga tgactctctt
     9241 agggtttgaa attgttgctt atgctggaga tgctcgctct aaacttttag aagcgcttaa
     9301 agcggctgaa aatggtgatt tcgctaaggc agatagtctt gtagtagaag caggaagctg
     9361 tattgcagag gctcacaatt ctcagacagc tatgttggct cgagaagctt ctggggagga
     9421 acttccatac agcgttacca tgatgcatgg tcaggaccac ttaatgacta ctgtcttatt
     9481 aaaagatgtg attcatcacc tcatcgaact ttataaaaga ggagcaaaat aatgcataaa
     9541 ctcattgaac ttattgagaa agggaaaccg ttctttgaga aaatctctcg aaacatctat
     9601 cttcgtgcta ttcgtgatgg atttatcgct ggtatgccag tcatcctttt ctcatctatc
     9661 tttatcctta ttgcctatgt cccaaatgct tggggattcc actggtcaaa agacattgag
     9721 accttcttga tgactcctta tagctattcg atgggtatcc tagcattctt tgttggtggt
     9781 actaccgcta aagctttgac ggactcaatg aaccgtgacc tacctgcgac caatcagatt
     9841 aacttcttat ctacgatgct agcgtctatg gtagggttcc ttttgatggc agctgagcct
     9901 gcaaaagaag gaggcttctt gacagccttc acgggaacaa aaggtctttt gacagcattt
     9961 attgcagcct ttgtgacagt taatgtttac aaggtctgtg tgaaaaataa tgttactatt
    10021 cgtatgcctg aagaggttcc accaaatatc tctcaagtat ttaaagactt gattccattc
    10081 actgtagcgg ttgttctcct ttatggattt gaactcattg ttaagggaac tcttggtgta
    10141 accgttgcag aatcaatcgg tacccttctt gctcctctct tctcagctgc agatggttac
    10201 cttggaatta cacttatctt tggtgcttat gccttcttct ggtttgttgg tatccacggt
    10261 ccttcaattg tcgaaccagc aatcgctgct atcacttatg ccaatatcga tgccaacttg
    10321 catcttatcc aagctggaca acatgcagac aaagttatca cttctggtac tcaaatgttt
    10381 attgttacca tgggtggaac aggagctaca ttgattgttc cattcttgtt catgtggatt
    10441 tgtaaatcag aacgtaaccg tgccatcgga cgtgcctcag ttgttccaac attctttggg
    10501 gttaatgagc caatcttatt tggtgcacca atcgtattga atccgatttt ctttgtaccg
    10561 ttcattttcg ctccgattgt caacgtttgg atctttaaat tctttgttga cacattgaac
    10621 atgaactcat tctctgccaa ccttccatgg gttactcctg gtccgttggg aattgtactc
    10681 ggtactaact tccaagttct atcatttatc ttagccggac tcttggtagt tgttgatact
    10741 atcatttatt acccatttgt taaggtatac gatgaacaaa ttcttgaaga agaacgttct
    10801 ggtaaaacaa acgatgctct taaagaaaaa gtagctgcaa acttcaatac tgctaaagca
    10861 gatgccgttc ttggaaaagc aggcgttgct aaagaagatg tagcagcaaa caataatatc
    10921 acaaaagaaa ctaatgtcct tgttctttgt gcaggtggag gtacaagtgg cttgcttgcc
    10981 aatgccttga acaaagcagc agcagaatac aatgttccag tcaaagcggc agcgggtagt
    11041 tacggtgctc accgtgaaat gttgccagag tttgacttgg ttatcttggc tccacaggtc
    11101 gcttcaaact tcgatgatat gaaggctgaa acggataaat tgggcattaa acttgcaaaa
    11161 actgaaggag ctcaatatat caaattgaca cgtgatggac aaggtgctct tgcatttgtt
    11221 caacaacagt ttgattaata aatttgtaaa agttatttga gacgagatta ttctttcaat
    11281 tccttataat attgttaacc atatcattgc atttcatttc cttggtttcc ggaactcaga
    11341 atatcgtctc aaatgctttt tttgaaagga tttatagtta tgactaaaac acttcctaaa
    11401 gattttattt ttggtggtgc tacagctgct tatcaagcag aaggtgcgac tcacacggat
    11461 ggtaaaggcc cagttgcatg ggataagtat cttgaagaca attattggta cacagcagag
    11521 cctgcaagtg atttttacca taaatatcct gttgatttag aattagctga aaaatatggc
    11581 gttaatggta ttcgtatttc aattgcctgg tctcgtattt tcccaactgg ctatggcgaa
    11641 gtaaatgaaa aaggtgttga attctaccat aatctttttg ccgagtgcca caaacgtcat
    11701 gttgagccat tcgtgacttt gcaccacttt gacacaccag aagttcttca ctcagatgga
    11761 gatttcttaa accgcgaaaa tatagaacac tttgtagatt atgccgcttt ctgttttgaa
    11821 gagttcccag aagtaaacta ctggacaact ttcaatgaaa ttggcccaat tggagatggt
    11881 caatacttgg ttggtaaatt ccctccaggc attcaatatg atcttgccaa agtcttccaa
    11941 tcacaccaca atatgatggt ttctcatgca cgtgctgtga aattgtataa agataaaggt
    12001 tataaaggcg aaattggtgt agtacatgcg ttgccaacca aataccctta tgatccagaa
    12061 aatccagcag atgttcgtgc cgctgagtta gaagatatca tccataacaa atttatcttg
    12121 gatgcaacct accttggaca ctattcagat aaaacgatgg aaggggtcaa ccacatccta
    12181 gctgaaaatg gtggagaact tgatcttcgt gatgaagact tccaagcact tgaagcagct
    12241 aaagatttga acgatttcct tggtatcaac tactacatga gtgattggat gcaagccttt
    12301 gatggtgaga ctgaaatcat tcacaatggt aagggtgaaa aaggaagttc taagtaccag
    12361 attaaaggtg ttggtcgccg aatcgcacca gattatgtgc ctagaacgga ttgggactgg
    12421 attatttatc ctgaaggctt gtatgaccaa atcatgcgag tgaaaaatga ttatccaaac
    12481 tacaagaaga tttacatcac tgaaaacggt cttggataca aagatgagtt tgtagataat
    12541 accgtttatg atgatggtcg tattgattac gtgaagaaac acttggaagt tttatcagac
    12601 gcaattgcgg atggtgcaaa tgttaaaggt tacttcatct ggtcacttat ggatgtcttc
    12661 tcatggtcaa acggatacga aaaacgttat ggactattct atgtagactt tgacacgcaa
    12721 gaacgctatc ctaagaaatc agctcattgg tataagaaag tagcagaaac tcaagtgata
    12781 gagtaatatt acttgaaaat gtaaaaaatt gttatccgat actttagaaa aagtacaaca
    12841 cttatttaga ccaggctttg aaaaggtata tattgttagt ttcgaagatt gtcctatgat
    12901 tcctgagctt gaagctaccc cgttgctaaa atgtggtaaa tggtatgttt caactggaaa
    12961 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
    13021 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
    13081 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
    13141 tttaaaacac ttggtggtca actgacttcg attaaagaca aggatgggat agagtatctc
    13201 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
    13261 agtttacgaa atgattgggc tatttataga cctcaagaaa gacccttttt tacaggtctt
    13321 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
    13381 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
    13441 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
    13501 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
    13561 cctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
    13621 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
    13681 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
    13741 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
    13801 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
    13861 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
    13921 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
    13981 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
    14041 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
    14101 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
    14161 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
    14221 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
    14281 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    14341 gt
//
LOCUS       DARBFD010000067        14101 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000067 DARBFD010000000
VERSION     DARBFD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.067"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            15..2180
                     /gene="acm"
                     /locus_tag="SCK08_001973"
     CDS             15..2180
                     /gene="acm"
                     /locus_tag="SCK08_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEG1201835.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2452..2937)
                     /locus_tag="SCK08_001974"
     CDS             complement(2452..2937)
                     /locus_tag="SCK08_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEG1201836.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3027..3372
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3417..4058
                     /locus_tag="SCK08_001975"
     CDS             3417..4058
                     /locus_tag="SCK08_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1201837.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4077..4712
                     /locus_tag="SCK08_001976"
     CDS             4077..4712
                     /locus_tag="SCK08_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1201838.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4730..5545
                     /locus_tag="SCK08_001977"
     CDS             4730..5545
                     /locus_tag="SCK08_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1201839.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5864..7300
                     /locus_tag="SCK08_001978"
     CDS             5864..7300
                     /locus_tag="SCK08_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1201840.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7489..9936
                     /locus_tag="SCK08_001979"
     CDS             7489..9936
                     /locus_tag="SCK08_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG1201841.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10166..11233
                     /gene="rlmN"
                     /locus_tag="SCK08_001980"
     CDS             10166..11233
                     /gene="rlmN"
                     /locus_tag="SCK08_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG1201842.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11426..12529)
                     /locus_tag="SCK08_001981"
     CDS             complement(11426..12529)
                     /locus_tag="SCK08_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1201843.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13082..13630
                     /gene="aac(6')"
                     /locus_tag="SCK08_001982"
     CDS             13082..13630
                     /gene="aac(6')"
                     /locus_tag="SCK08_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1201844.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4829 a         2342 c         2969 g         3961 t
ORIGIN      
        1 gggagataga aaaaatgaaa aagtgtagaa atattatttt atcaatgtta tttattatta
       61 ctaatataac atcactgatt cctgttcatg tctatgcgga tgcaggcaga gatatcagca
      121 gtaatgtcac ttcgttgacc gtggacccaa cgaatattac agatggagga aacataaaag
      181 taaaattctc gtttgacgaa aaaaaacaaa atatccaacc aggtgattat ctttggatca
      241 attggccaag tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga
      301 ttgaaaataa aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta
      361 atgaaaatat taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca
      421 gaaacgtaac cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata
      481 aaacagctac tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt
      541 ataaaacagg agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta
      601 acaataacgg cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac
      661 aaagattaga tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt
      721 atagaggaga agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt
      781 tttcagtaac tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga
      841 aaataatggt gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc
      901 attcggaagc ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc
      961 atacagttaa aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga
     1021 agattttcaa atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa
     1081 gacgagcaga tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac
     1141 agggagaagc tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag
     1201 cacccaattg gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg
     1261 aaaatgatac agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca
     1321 cagcaactaa aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat
     1381 tcagagcaag tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca
     1441 atggaataac ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat
     1501 atacgtttaa agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc
     1561 aaaaaattga aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag
     1621 gacaaaaaac atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag
     1681 taaatctatt agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt
     1741 ggcgctatcg tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg
     1801 taacggaaga caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca
     1861 gttatacacc ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa
     1921 attttccaga gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa
     1981 tatttgctca tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg
     2041 tacctaaaca gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat
     2101 ctagttttgc tgtaatcatt ggtttattga tagtaactat ctgtgctggg atattcttac
     2161 aaaaatataa aaaagtttag ttagaatata atattttttg ttaaatatga ctgaaatcaa
     2221 aagaataaaa ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt
     2281 cgtagaatca ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag
     2341 cgttcatact agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc
     2401 taaataaaga aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt
     2461 ttcataaact ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat
     2521 aatcaacgta gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc
     2581 agcatcgatc acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac
     2641 tgtacctgct ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg
     2701 cttttctttt aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat
     2761 gacaagatag atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc
     2821 aggtagggaa aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa
     2881 agagataccc agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc
     2941 gcatcaccct ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat
     3001 catgtaaatt acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt
     3061 gtttttagag agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg
     3121 gagtcgattt tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg
     3181 ttgctagatg accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac
     3241 cacgtgatta acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc
     3301 aacgactgtg tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa
     3361 tacgtcctct tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg
     3421 agtatttgat tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat
     3481 ttttatttac cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt
     3541 tgcattttaa gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac
     3601 cgctgttgtt gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg
     3661 atcgtttcga agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga
     3721 tctttcgtgg agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc
     3781 aattaagtcc ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt
     3841 taccttctgt agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat
     3901 taggtttagg tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac
     3961 tgcctttgct aggcgtggca atcatctatc ttacattgac tggtattcta acaatcataa
     4021 tgaaacaagt tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga
     4081 ttgaattgaa gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc
     4141 taatcattcc agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca
     4201 cgcttttacg tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg
     4261 gtgtaccgtt caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc
     4321 aagacttcca gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa
     4381 tggtattgaa acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat
     4441 tagggattag tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc
     4501 gggtagcgat tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa
     4561 caagtgcttt agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag
     4621 aagatggggt aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg
     4681 atcaattact aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac
     4741 cagttttcta ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa
     4801 aacttcagca gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga
     4861 cgatacattt gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat
     4921 tgatttagcc aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga
     4981 ctggtcaatg aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta
     5041 ttccaaaacg ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga
     5101 tcaagtgctg atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa
     5161 gagattaggt gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt
     5221 attaaaagag atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt
     5281 gatcgatctg aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta
     5341 ttatttgaaa caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag
     5401 tgaggatttt gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga
     5461 agccctagat caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg
     5521 cgaagatgtt acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt
     5581 ccctttttcc gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc
     5641 aggctcacaa cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat
     5701 ttatgcaaaa atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg
     5761 actttttcac aacctccttt acataatttt attttgttgt ggaaggggtt tcgtgttatt
     5821 ctaaaagagg aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact
     5881 tacctcattt ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg
     5941 agcttggcaa gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg
     6001 aaagacattt aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga
     6061 agatattgct ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac
     6121 acggattttt cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag
     6181 attgatcgac gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga
     6241 tcttccacaa gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga
     6301 tttcactcat tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat
     6361 tagtttgaat gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc
     6421 aggtgtgaca gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc
     6481 atctgtcatc aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc
     6541 gtatacgcca aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc
     6601 agaagacctg atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc
     6661 tgccatgagg ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga
     6721 attgctagaa tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa
     6781 tgcattcaat ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg
     6841 ttcatcagaa gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga
     6901 acgaacaaat tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat
     6961 cacaagccgt taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa
     7021 gttgacagaa gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt
     7081 caaagccatc aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc
     7141 ttatacagat ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt
     7201 tgatcaagac gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg
     7261 gtaccaaaaa ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga
     7321 aggtacggct ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa
     7381 ctgtaaaata agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc
     7441 ccttactttt ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag
     7501 aaacggccta ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag
     7561 gaacaaatgc tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt
     7621 ttaaaattag gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat
     7681 acttaccaaa tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat
     7741 acagagtttt caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt
     7801 atcttattat tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc
     7861 atggggacga catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac
     7921 gttggtggtg ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag
     7981 cctggaagtt atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat
     8041 ctggaaggct atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta
     8101 ttggaaggtg atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa
     8161 cagcgcgcag aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa
     8221 gctgcagaat atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa
     8281 tggcaggaac cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt
     8341 caagaaaata tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc
     8401 aatacagcag aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt
     8461 gagattgaag cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat
     8521 tctataccaa ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta
     8581 ttagaacaaa ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga
     8641 ccatccgtca caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg
     8701 aacttaacag atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg
     8761 attccaggta aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc
     8821 tttagagaaa tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta
     8881 ggacgtgatg tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc
     8941 gtggctggtt cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata
     9001 ttaatgcgtg ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa
     9061 ttgaatatgt acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa
     9121 gcagcgcaag ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg
     9181 gcaaccggag taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa
     9241 aacggaacga aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat
     9301 ttgatgatgg tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct
     9361 cgggcagcgg ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca
     9421 ggaatcatca aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat
     9481 tctcggacga tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta
     9541 ttcttgccaa tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac
     9601 gaagtagaac gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa
     9661 atgatgccga cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg
     9721 tttgaagaag caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa
     9781 agaagatttc ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat
     9841 ggagttgttg gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa
     9901 acatcagaaa cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa
     9961 aacgaataac caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc
    10021 catcttttct aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta
    10081 ttcgatttat ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa
    10141 ctttgagaaa aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga
    10201 agaattaatc aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg
    10261 ggaatggttg tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca
    10321 attgatcgaa aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca
    10381 agaagcttcg gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga
    10441 gactgtactg atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg
    10501 taatatcggc tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc
    10561 tggagaaatt gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg
    10621 cgaacgcgtt tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa
    10681 tgtaatggac tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca
    10741 cattacagtt tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt
    10801 gcaagtcaat ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat
    10861 gcggatcaac cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga
    10921 gaaaacaaac cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc
    10981 agagcatgcg caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa
    11041 cttgattcca tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga
    11101 tgtattgaga ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga
    11161 acatggtaca gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac
    11221 agttgccaaa taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct
    11281 taattaagga actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc
    11341 tttttttgac aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct
    11401 gcttgatggg ggttttagca attatttaac tgattttctt atatccttgc tggctttctt
    11461 gtgtagcatg ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt
    11521 cataaggcac atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat
    11581 cggtcgtgac attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa
    11641 acacatagat tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac
    11701 cataactgac agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt
    11761 aatagaatga gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc
    11821 cggtagtaac aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg
    11881 cactatcttt caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga
    11941 tttggattcc taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt
    12001 cgacttcttt ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg
    12061 caataattgc tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt
    12121 ccattacgcc ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac
    12181 caatagtggg aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat
    12241 cgcaagcacg gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct
    12301 ggatcaactg ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa
    12361 cctctatttc ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct
    12421 tccctccaat cagcactact tttgtcccat attctttgac gattccattt gctttttcat
    12481 acgcatctgt tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct
    12541 tctttcttcg ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag
    12601 atggagtaga ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg
    12661 aaatcatcaa taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat
    12721 gaatttttgt tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa
    12781 aattctaaaa attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt
    12841 tttttttaaa gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat
    12901 tgatggcttt cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt
    12961 tataagtaag ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag
    13021 cgagaacatt tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg
    13081 tatgataatc agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt
    13141 actgagactg acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat
    13201 gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat
    13261 tggtgcaatc cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag
    13321 ctcccgacga aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc
    13381 ttccagagga ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt
    13441 aagtcaaacg gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg
    13501 tgaacatccg tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa
    13561 tgcaaatggc tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa
    13621 ttctcaataa taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat
    13681 ttacgaaaat tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt
    13741 ttggaacacc gtgtaccaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa
    13801 ttttgtgaaa atggctttct acatttttgc ataaatttgg cttaataccg agaaactggc
    13861 ttttgaacac cgtttattca gataagggac tgtgacaaga cttttgtcac agtttctttt
    13921 tgtttattct tttttatctt tgtatggcaa taatagacaa tataacgaaa attatttaat
    13981 ggcttttgga agaccatgga aaaccttgta caaagctatt ttttctgaaa aattccttga
    14041 aacctcacga aaaaatcatt attttatgaa aaagacaaaa aaaagtgttg attttttaat
    14101 a
//
LOCUS       DARBFD010000068        13917 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000068 DARBFD010000000
VERSION     DARBFD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.068"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            131..505
                     /locus_tag="SCK08_001983"
     CDS             131..505
                     /locus_tag="SCK08_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1201845.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="SCK08_001984"
     CDS             complement(619..1248)
                     /locus_tag="SCK08_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1201846.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="SCK08_001985"
     tRNA            complement(1382..1454)
                     /locus_tag="SCK08_001985"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="SCK08_001986"
     tRNA            1668..1741
                     /locus_tag="SCK08_001986"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="SCK08_001987"
     CDS             complement(1879..2043)
                     /locus_tag="SCK08_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201847.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="SCK08_001988"
     CDS             2338..3666
                     /locus_tag="SCK08_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEG1201848.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="SCK08_001989"
     CDS             3868..4701
                     /locus_tag="SCK08_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEG1201849.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="SCK08_001990"
     CDS             4717..5454
                     /locus_tag="SCK08_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEG1201850.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="SCK08_001991"
     CDS             complement(5609..6577)
                     /locus_tag="SCK08_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEG1201851.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="SCK08_001992"
     CDS             6809..7642
                     /locus_tag="SCK08_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEG1201852.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="SCK08_001993"
     CDS             7676..8515
                     /locus_tag="SCK08_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1201853.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="SCK08_001994"
     CDS             8541..9101
                     /locus_tag="SCK08_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1201854.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="SCK08_001995"
     CDS             9300..11021
                     /locus_tag="SCK08_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEG1201855.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="SCK08_001996"
     CDS             11186..12328
                     /locus_tag="SCK08_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1201856.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="SCK08_001997"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="SCK08_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEG1201857.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DARBFD010000069        13812 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000069 DARBFD010000000
VERSION     DARBFD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.069"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..234)
                     /locus_tag="SCK08_001998"
     CDS             complement(<1..234)
                     /locus_tag="SCK08_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1201858.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSD"
     gene            complement(713..1432)
                     /locus_tag="SCK08_001999"
     CDS             complement(713..1432)
                     /locus_tag="SCK08_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1201859.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1481..3127)
                     /locus_tag="SCK08_002000"
     CDS             complement(1481..3127)
                     /locus_tag="SCK08_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1201860.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3306..3896
                     /locus_tag="SCK08_002001"
     CDS             3306..3896
                     /locus_tag="SCK08_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1201861.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3988..5493
                     /locus_tag="SCK08_002002"
     CDS             3988..5493
                     /locus_tag="SCK08_002002"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEG1201862.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5577..6929)
                     /gene="celB"
                     /locus_tag="SCK08_002003"
     CDS             complement(5577..6929)
                     /gene="celB"
                     /locus_tag="SCK08_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1201863.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6929..7447)
                     /locus_tag="SCK08_002004"
     CDS             complement(6929..7447)
                     /locus_tag="SCK08_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201864.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7463..7789)
                     /locus_tag="SCK08_002005"
     CDS             complement(7463..7789)
                     /locus_tag="SCK08_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEG1201865.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7802..9781)
                     /locus_tag="SCK08_002006"
     CDS             complement(7802..9781)
                     /locus_tag="SCK08_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1201866.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9802..10116)
                     /locus_tag="SCK08_002007"
     CDS             complement(9802..10116)
                     /locus_tag="SCK08_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEG1201867.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10284..10577
                     /locus_tag="SCK08_002008"
     CDS             10284..10577
                     /locus_tag="SCK08_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEG1201868.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10655..11767)
                     /locus_tag="SCK08_002009"
     CDS             complement(10655..11767)
                     /locus_tag="SCK08_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1201869.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            12154..12333
                     /locus_tag="SCK08_002010"
     CDS             12154..12333
                     /locus_tag="SCK08_002010"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201870.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12527..12697
                     /locus_tag="SCK08_002011"
     CDS             12527..12697
                     /locus_tag="SCK08_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1201871.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13121..>13812)
                     /locus_tag="SCK08_002012"
     CDS             complement(13121..>13812)
                     /locus_tag="SCK08_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1201872.1"
                     /translation="DDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGV
                     YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQ
                     GAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKI
                     EKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVF
                     ISIKFRKKRNI"
BASE COUNT         4332 a         2812 c         2359 g         4309 t
ORIGIN      
        1 atccgaagaa aataaaaatt tgaaattttc ccaacccacc caaggacttt ccctcaagct
       61 agccaagaac ccatctgctg aaatatgaaa atttttgaat gctacgacat tcgccattac
      121 aggtatgtaa aagaaaaaaa tcatccaaag catacccggt acagccatca ggacaagtgc
      181 tttataacgc catagattca taaaaaaatg attgaacccc tttcgttttt tcatctaaaa
      241 acccctttcg attcattcgc aacgtaatta tagcgctttc aataatgaga ataaataaac
      301 aaagtatagg gaaaatgaaa caaagtatag gagaggttgt gaaatcagcg ttttggcctg
      361 agtattagag aaaaaaacgg gaaaatagtt cctcatattt tgccgttttt tgagctaata
      421 ccgagggtca gctgattgaa caccgtttat cagaggttgt gaattcagcg ttttggcctg
      481 agtattaggg agatcgtgtc cttactatct aactctgagt cacaaggaac aatggggact
      541 gtaacaaaag tcttgtcaca gtcccttatt ccgaacaaac ggtgttccag aagtagcttc
      601 tacggaaata agccgaaatt tcacaaaaaa gccatccgaa agtcagacgg acaagtctgc
      661 ttttcagatg gcgctacaac tactctgctt attcttttag ctatccattc aattattttt
      721 ctctcacttg ttctagctct tctttggtca tgggtcgata ttgcccttta ttcaaatctt
      781 taggcagcca aaattttccc atacggattc tttttaaata taagacttct tttcctactg
      841 ctttgaccat tcgcttaact tggtgaaact ttccttcatg caaaatcaga cggataattg
      901 aagtttctgt ctcttgatct acttgatcaa tttggagttt tgccggcaat gtctgctcat
      961 cgccatcaat aatcagtcct tctgcaaata aacggatatc ttcttctgtc attataccgc
     1021 taacctcagc tagatattct ttctccacat gttttttagg tgatagtaac tgatgggcaa
     1081 acaccccgtc attcgttaaa atcaagaggc cctctgtgtc tttatctaat cggccgactg
     1141 gaaaaagatc ttcccgataa tcctggtctt ccaataaatc gattacggtt tgatcatgat
     1201 tatcctgagt agcagaaatg actcctgcag gtttatgcaa aatgtagtaa aaatcttttt
     1261 gatacgtaat gatttctcct aaataagtaa ccgcatcttc gaattcattt acttgcgctt
     1321 tatcgctttt caccacttgt ccattgactt cgatcgttcc ttttttgatt aatcctttga
     1381 cttctttgcg gctgccaagt cccatatctg ctaaaaactt atctaaacgc atgcgtttcc
     1441 cccatttcaa aagagcatca atctagctct tctgattcac ttattttatt tttaatttat
     1501 gtcgtaaacg cgctattcct ggtcctagta gtctttctgc caagcggatt ttcaaagcta
     1561 aataaccata cactaaagcc cctatacctg ctactacaag aatcaacaat agagactgga
     1621 atttgctatc ttcgtttaaa aaagcaccaa aaatcatctt tgtgatccac gaagccaaaa
     1681 gcataatcaa ggtaagaata aagatcagca atgtcctgcg ccagacaaaa cttggtttaa
     1741 agcgggctac ttcatggatt ctctttaaga tcaaggtaca agacacagca aatccgatca
     1801 ttgtcgccaa taaagggccg tatacttcaa ataaacgaat ggctgggtat tgaagaagga
     1861 gtttcactag aaacccaata accaaataat taattgccgc cttgttttca aacattcctt
     1921 gcagcatgtt ggaaactagc atatatagtc ctaggaagag tcctacaaaa cttgcttgga
     1981 tcagtacatt acttcctaga gaatccggtg tataaaataa tgtatttagc ggataggcaa
     2041 gtaacataac accaaatgtc gccggaaaca taaaaaatga aaacaactgt aaatttccgc
     2101 ttgtcaattt tgccagacct actcgatctt tcaacgtaac agcttctgta atcaacggta
     2161 acccagtaga cgcgatcgat gttgccaatg caataactac catcgtcaac ttatctggat
     2221 tggcactaaa aatcgaaaaa agatccagta actgtgcatt ggaatactcg gtggatgcag
     2281 acatgatctt cataaaagtg aactgatcga ctaatttgaa gatcgttacc cctgaaccca
     2341 caataataaa cggaatcgct tctttaacgg tatctaacaa caattccttc gttgcaatat
     2401 ttattttgtt ttcgctgtag tgaataaacg ccgatgtata cgcttgatgt ttcttcaaaa
     2461 aatacaaaag gacaagaata cttgctagca taccgataaa tgccgcgaaa gttgattgcg
     2521 tgaccgccgt cacatagtca ccatccatga ctttcatgat gatgaatgta gccaatagca
     2581 tataaaaaat acgtgccacc tgttctacaa tctgcgacag cgcataaggc atcatttctt
     2641 gattgccttg caaataccct cgtatcacac tcatacaagg aaaaaccaat acagcgatac
     2701 tcaaagaacg catcgttgga atcagttctt ctccgccacc agaagcatga gctagccaag
     2761 gagaggcgat gtacatgaac aaagcaaaca acaggcctaa cccgcccatc atctgtaatg
     2821 cacggataaa taatcgtcgt gaagtgccat attcattcaa tgaattatag cgtgcagttt
     2881 gttttgcaat cgcagcaggt attcctgctg tagagatcag tagaaacaat gcatatatat
     2941 tgtatcccat attgaataat ccattggcag ccttagcatt ttcgcccatc caagcatacc
     3001 agggaatgat atagacagcg cctagcaatc ttgaaatgat attactggcg gtcatccaag
     3061 cagagcctcg tgccattttt tcttgcggtg ttaattgatc taatgctggt tttctttgat
     3121 tatccatcgg tcagattcca actttctaca aaacattact atcattttaa ttggtaaaac
     3181 agaaactggc aatcgatttt atataacttt tataagaaca attgtttttt tgaattcggt
     3241 ttcgtttttt tggtaaagta aagaaatata cagcctttta tggctaacat ttataggaga
     3301 tatcaatgac acaagaaata aaaaaactac ccgttatatt tttaggtctt ttctttatca
     3361 cagttggatt gaattggttc ttgctccctc acgatatagc ttccgccgga gtgggcgcca
     3421 tcggacattt agttgaaacg gccttttcta tccaacgctc agttactgtt tggagcatca
     3481 atcttatgat gctgcttcta gccgctgtcc tattaggaaa atcagtattt tttaagactg
     3541 tagcgggaag tctgcttttt cctgttgttt tagagattgt tccaaaagtg gaaatgatct
     3601 cctcacattt cttttcactg atttcaggaa gtcttttgtt cagtcttggg gtttacacat
     3661 tgtacacagt cggggcctca aatggcggta taacgatacc gccgattatt ttccacaaat
     3721 tttttcggct tccaatgccg caaggacttt tacttaccaa tagtcttatc gtctttttga
     3781 attattatgt ttttggattg ttggagacac tattcgttct cctatctatt acagtcagtt
     3841 cattgtttat gaaaatactc atccgcttgt cccctgtgtc aagaaaattt gaataaagct
     3901 ggattttgtc atagcggaaa caaacgatat attaaaaaga gtgtgctata ataaaccaag
     3961 aaattgaata aagcgaggga tcaatcaatg agcttctctt tagaagacat ccgtcattta
     4021 ctattaaaag aaaatctatt aaaagaattt gtttcaacaa gaggctggca tcttgatgta
     4081 ccagaaaaag ctgaatttct tcagttaagt tatgattcaa gaaaagctga tgcttctact
     4141 ttgtttttct gtaaaggaat gaatttcaaa gaagaatatt tggattcagc aatcgaacag
     4201 ggtattcaat attatgtatc agaacaacca tacgaaaatt cagcagtagg aatcatcgta
     4261 acggatatcc gtaaagcaat ggcgcttctt gctatggctt tttacgacta tccgcaaaat
     4321 aaattgaaag tgatcggatt cactggaaca aaaggtaaaa caactgctgc gtactttaca
     4381 aaagcaatct tggatcacac aacaaataaa aaaacagcat tgctttctac tatgaataca
     4441 acgttagatg gcaaaacata tttcaaatcc catttgacaa cgcctgagtc attagattta
     4501 tatcgtatga tgtcagaagc agttgaaaat ggtatgactc atctagtgat ggaagtttct
     4561 tcacaagctt acaaaacaca acgcgtatac ggcttgactt tagatgtcgg aatcttcttg
     4621 aatatctcgc ctgaccatat cagtccgatc gaacatccga catttgatga ctatttctat
     4681 tgcaagcgac aattgatctt gaattcaaaa acagttgttt taaatcatga aagtgactat
     4741 tttgatttac ttagggaaac agccgatcta ttccagattc caacgatcac ttatggtcgt
     4801 tcggaagaaa gcaattatca agtgatccgt tcagataaag gtactcacgg ttttacttta
     4861 tcttctcatg aagatcgctt agctatctgc gatactgctt atgatatttt gctggctgga
     4921 ggattcaacc aagaaaatgc agccagtgcg atcattgccg cagcactaac aggtgcctcc
     4981 agtcaagatg cacaagacgg attaaaagaa gctcgtgtac caggccgaat ggatcaattg
     5041 atccaatcca atggagcgca tgtctatgtg gactatgcac ataattatct aagcttgaaa
     5101 acattgctga aatttgccaa aaatgaacat ccagatggcc gtgtaattgt tgtattaggc
     5161 agcccaggaa acaaagcaat ctcccgtcga catgattttg gaaaagtgct atcagaaaca
     5221 gctgacatcg cctttttgac ggcagatgac ccagcatttg aagatccgaa aaaaatcgcg
     5281 gaagaaatca atgaagcaat tactaatcca gatttgatcg ttcattatga aatggatcgt
     5341 cctgaagcga ttcgacgcgc tttagcagaa agcgctccgc aagattcagt cgttattgcg
     5401 ggaaaaggcg tggatcccta tcaaaaaatc aatggtgtag atgaacctta tgaaggggat
     5461 tatgcaatcg tgaagcggtt gattgaagaa taatggtatt tatatactag tggtttccca
     5521 tttctaagaa aaaaagggac tgtgattttt tgtcacagtt ccttttttct tagaaatcat
     5581 tttaactttt cactagctaa ttctgctgcc ttttctcttt cttcatttaa taatttatta
     5641 tcatagaatt tgataaatgg gaaccagata atgaatgcga tgattgcaca aatgactgct
     5701 aacacagcag ctttccaatc accaccacta ccaacaaatg ctccgatccc gactggtgaa
     5761 ggccacggca tctgtgcaag catcggtttg acgatttcca gccgaatggc aaaataagcg
     5821 agtgtagcag aagccatcgg agctaagaaa aacggaaccg ctaaataggg attgtagacg
     5881 attggcatcc cgaaaatgat tggttcattg atattgaata atcctggtac aagtgaggct
     5941 tttcctaaca ctttcagctg atcagatttc gcgaggaatg caataaagat gatcaatcct
     6001 aaagtcgcac ctgatccacc aacggttaca taagaattgt tgaattctcc tgccaacgga
     6061 taatttgcgc cttgtgcgtt agctgccata ttagctaata cgatcggggt caagaaagaa
     6121 gtgatgatat tcgctccatg gatccctacg atccacaatg catggatcaa gaaataaatc
     6181 accatgacac caagccatgt attcgtcaac tgcgtaacaa acccaaatgg gatagcaatc
     6241 attttgaaga tatccgttcc tagtgcaact aataatccat tgatgatcat caccacaaac
     6301 gcaatgacaa atgttgggat cagagcagtg aaagaacgag aaactcctgg cggaacaaca
     6361 tctggcattt ttatgaccca atttctcttg acacacatac aatacaattc gaccgcaata
     6421 atcgacataa taatagctgt aaagattcct gaagttccta aacgttcgac aaaagagccc
     6481 atcctaatac catttactac agtttctgtc tcattgatag aagcaatcaa gctgatttta
     6541 ccatcagtca tgatcaattc agggatacac ataaagaatg caaaaacaga tagtaatgcc
     6601 cccgtgagcg gattaacatt taatttttgt tcatctcgtt caattgacgt taattcataa
     6661 gcaaacacaa tggcaaagta aatagataaa atccccatcg tcattgtatt gacgatcatg
     6721 tacaagtcac taactttgaa aaaagttgca ttaaaaaatc cttctaaaaa tgggaacggt
     6781 aatggcaaaa tatttaaaac caaaaacatc gagcctacaa tcgtgaaagg aatagaagcc
     6841 atccccgctg ccatgacagc ccgaacgatt ttaagttgcg caattttccc cataggtccc
     6901 atcaggtatt tctgcaaaaa atcaaacatt agttattctc ctttcctaaa tataacgctt
     6961 tttcatattg tttaatacgg atatactgtt ggtcttggtt gagtgaaatt ttctttaaac
     7021 gatactgtat aaatgcactg gcaaaaatac cgcttacaac aagaatgaaa acaaataact
     7081 gtcctccatt gttatttgcc ataaaaggat acaagaatgt aaatcctgag gtaaacaaca
     7141 ctggaatcac taataaatta aagacactct ggaacaaaaa ataccacttt gtccatggaa
     7201 cgttcgtttg gtgttttcga taaagcttta cttgttctcc tactgcaggc aaaatagcta
     7261 aaatcagaat catcggtaaa ctacttccta aacttttagc tgatagaagc agaatcatcc
     7321 attgcatgtt catgaaaaag aaaaaggcag tcaggtaacg tacgattaga taacgattga
     7381 aatacatgtt tttcaaacta agttgctttt tttctaattc gattttcctt tttactgtat
     7441 cctgcacaat gatccttcct ttctatttat ttttttgata caaataattc atttcctgtg
     7501 caacttccaa taatgtcatc gtcgtcatca gatgatcttg tgcatggacc atgataattt
     7561 ccatttctat ttttgtcccg ctggcatatt ctttcagaag ttgagtttgt gactgatgcg
     7621 catgaagaat ctcttcattt gcttgtcgca acttctcttc tacttcttca aacatctctt
     7681 tacgcatcga gcgaaatgct tcatgtatca gtgtccgtgc atttccgcta tggaaaatga
     7741 tatcaaaaga agtcacttgt agtgtttcac tgtttatttt ttcagccatc tgttttcctc
     7801 cttattcgat cagtttgata aatttactgc tgaaattctc aaaaataggt tcagctaaga
     7861 tttcctgctg tacttttagc tggtcaacta acctaacgat tgctttagtc acgaccgtaa
     7921 ttccttcatt ttcgacataa gaaggcgaaa ctaaaaaaac aaaatgaatt tcttgatatt
     7981 gatcccactg catcccagaa ggaatcaatg caacggctac ctttgtttcg attccaactg
     8041 gaacagcggg atgtgggact gccactgtat cactgaatat gatttgtccc atattttcac
     8101 gttgctggat ctgctttcgc atttctctta tatatccttc ttcttcattt atgctcagct
     8161 tttgcagcaa atcatgaaga atttcttcct ttttcggttc gtacgtataa gtcttgaaaa
     8221 atttttcaga caactgttcg gtgtaaatct gctttttttg ctgtagagaa aatgctggta
     8281 tcggtctatc tgaggaatga tagggtatag attctccaac tacctttcgt attttacgaa
     8341 catcatcttc attcaaaaaa atactgacat gtaaaactgg aattttgaaa aacattgtcg
     8401 aaagatcaat agaagaaata atcaggtcga ttccttttaa agttgtttca ttgatctcat
     8461 aatagccttt tacacttacc actgtaatat ttttaccgaa ttcactgact acgcgattct
     8521 ttaatagttg ggcactgccg taacccgttg cacagataat caatgcatgt agtttatgcg
     8581 cagctctttc tttttccaat gccgccatga gatgcaaagc caaataggcc cattcatctt
     8641 cattgatggt atagtttttc aaagccggca tgttgctgaa atgccttttt actgcgtcaa
     8701 agactttggg attttctttc tttatctcct cagtcaatgg gttctcaatg cgaatacctc
     8761 ggctcaatcg aatcatcatc ggtgacaaat gatcgagtag tccattgaaa agctggagat
     8821 cctctactaa tgaaaaatga aattcctgag acaatctttt cagaactttc gctaattcct
     8881 ttgatagttc ctgatcattt tgattttctt tgtgattcga cttagccatg agatgtaatg
     8941 tcaaataagc aatttcttct tcgggaaacg atacacctgc gattacctca atacgttgaa
     9001 cgatccgctg cgccacttga tactctactc gcttagaaac ttcttgttcg atgcctaatt
     9061 ccttgatttc aaacccttct cggagccgtt tgatccccaa agccaagtgt aaagtcagat
     9121 tctgtatgat aaaatccgaa acttttaact tagcttctct tgtttcatcg agaatgataa
     9181 tgactagctc ttcaaaactg atgtctggga aaaaacggct ttgccctaaa tattctttca
     9241 atgaatttcc ataatggttt ccaaaaaaag tatccatgat aaaatgccgt cgatttcttt
     9301 cttccccttc gatccatgtc ccttgaccat gcttagaaac gatttttagt gaatatggaa
     9361 tcaatcgctc cttgatttct tgcatgtcct ttgtcaaact ggaacgactg atataaagct
     9421 cttctgccaa atcatccata aaaagaatgg catcttccaa aagcaattgg ttcaatatat
     9481 acttttgtcg atcttcggac tcataaaatc cttgcggttc tctagcatat ccaggtacta
     9541 tttcttgccg actcaaaaac aagtcaaatg tcagtctttg taaaatatgc agttggtagc
     9601 cataacctgg tttagcaatg atcttacctc cgtttttttc tactaactct ttgatccgct
     9661 gcaagtaatt acggattgtc cgatcagata ataacaattc agaagctaaa cgttgactgg
     9721 tgacatactc cttcttgtga tcaatcaaat atctcaacag caatttttcc ttctgtttca
     9781 ttatcatcca ctcctattcc atcatatgtt ctcttcgatc acctctgcta acttctggat
     9841 gcctgtaggg attgggacat acgcttggaa agggatacta acaacaggct ttccaacaga
     9901 ttctcctaat tttttgaatt tatccaaaaa catcgtcgtt tgcgggctga tcagaaataa
     9961 atcaaaatca ctattcttaa tcatattatc gccttctgta gctgaaactg catctacgag
    10021 tacctcttct cttttttgct taaagaaatc cgttgtcttt gaggccatca tcgaggatga
    10081 cattcctgcc gcacaaataa tcaatgcttt tttcataaat caatcactcc ttttattttt
    10141 ataaaccaag catatcttgt aagcgttttc tattcaaaat atatatttcc gtagcattgc
    10201 ggaaaaaatg tgtatcatat cttgaataat aatttctggc accgtgatat actagtgata
    10261 gaattttttc aggaggggat ttagtgagta tcacagtgaa tattttgtat acaagtacaa
    10321 atggacaagc cagaaaattt gcagaagaaa tggtcgaaag aaacattgtc aaagcgattc
    10381 gtgcagaaga aggaaacgag aaatatgact attactttcc actagaagat ccagaatctc
    10441 ttttactgat cgaccgttgg aaagatgaag aagcaatcga aaagcatcac aaatccgaaa
    10501 tgatggctca aataaacgaa ctccgtaaaa agtataaatt aaaaatgaaa gtcgaacgtt
    10561 tcactgattt accgtaaaaa gatacacatg gaatgatagc aatttatcat ccatgtgtat
    10621 cttcactttt tccttatttt ttattcgatc tcatctaatg attcaaaccg tgtttttctc
    10681 ttatttttcg gataagtatt tcagtcaccc caatatacat gattcctaaa cataccccaa
    10741 ggatattctc aaaaatgcgg atcgttactg ctcctggaac cccgaataaa ctggcagtga
    10801 ttgccaaagc accaaaagaa ttaaaaactg ttttatatct ataggtagaa caaacaccta
    10861 aagctagacc acctaatata ccgacccagt taaaaggaat aaattggctg ataccaataa
    10921 ataaaacaca tccaatcaat gagccaatga ttcggtctac tgctcgttct ttgaaaccaa
    10981 tcgacatcaa tccatagcta gatacaatag aagaaaatgc aaaccctgcc cacatcaatc
    11041 gctgaaaagg gacatactct cctgcaacga tcaaaagact aatgccaaat gcatagatga
    11101 gcatccaaat tttttcctgc gaatacatgc ctttcaaaaa aatctcttta aacaagcttt
    11161 tgtctttgtt cttttctcga tgttttctgt aaaggagaac tgaaaaccaa caaaaaaaca
    11221 aaaataataa gctacttgtt tgtgtaaaga aattcttgtt caacgaatct ttaggtaacg
    11281 aataaacaat aaagagatat gaaaagccgt acaaagaagc attccccatt ttgggatcac
    11341 tagcggttgc cattaatatg ctgcttagta aaataaaatg aatcaacatg cccataactg
    11401 accatttgat cagctggaca tacggtgcta ctaatagtga gaacatgacg attgctaaac
    11461 ctatgatcga atgggaaatc ttataaccaa agtcaacaaa acgaatactc atcaacatac
    11521 agaataaaac cacagcgtat ggtttatgct tcgattcaaa taatgttgta attataatga
    11581 taaaacaaat ggcaaacaat acgatgaata atgatcgtaa aaacaatgaa caaatataca
    11641 tgcacttttc ttttttagaa gctgcttctc gtattttttg ttttaaaata aacggatcta
    11701 gttgtagaga ctgataaaaa tccacctgtt gtcgtccttt ctttttactt ttatttttta
    11761 cagtcatcta tgacaggttg taaatactat ggaacatcat atggcgttgt cattttcttg
    11821 tcaactattt ttcctattca tgtttttata aaaaaacata tcgttttctt gtatgattaa
    11881 atcaccacaa aataaacaac tgagagaacg atatgcttta tgaatggttc tatcaaaaaa
    11941 attctgagat caatagacaa aaatttgatg attacagagg tctcttacga aaccttacag
    12001 aagaaaaaaa cattgatcgt tgctgcagtt ctcttccctg ctccatgtgt ctgtagaaat
    12061 tgtggttctg ctgtggtaga tggaaatagg aaattaatgg tggtgaaaaa caagaagaag
    12121 gaaaccattg tccactttga ataatataat catatgccaa tggttatacg cctaaaaaag
    12181 cagcgctata cctgtaaaaa ctgtcgaagc cattggaacg ctcaaagtta ttttatccgt
    12241 ccaaggcact cgatttcaaa tcatgtcaga cataaaatca cttctttact gactgaaaaa
    12301 gtgtcgttat tttttatttc gaaaagttgt taggtatctt tgacaaccgt tattcgcaca
    12361 ttgaaagagt tgagaagcta tttaccaaag tcttccaaaa gaattctgtc ttgagtgttg
    12421 agggttgtga atttcgttct cacgtttccg ttgaggataa actgagtttt atttccgcgg
    12481 atggcgagac agaaaaatta atcgagaaaa tttacctaca caaaaaattc ctcgattaac
    12541 aaactatttc cttagctgca ctaatcctaa agaaatagaa ttcttagtta cagacatgaa
    12601 cgccacctac tttcagctca ccaaacatgt tctaccaaat gcgaaattgg tgattgatcg
    12661 attacttggt ttttccacgc ctttcaagga ggcgtaacct ttattcacga gttggttgaa
    12721 gctttccgag acaaaagacc cgacttgttt ttctccttat tgacaaagag ccattaaaac
    12781 tagagaaaaa attacaactg atcgctgatt atttgtagaa agaaaatcaa agtattaccc
    12841 aataaacttg agtcattagg caaaaaacac tgcgaaactc aagaatgatt ttcgcagtgt
    12901 ttttgtatat tttttattaa ctagtattac tacaaaggtt tatttttcac ttctcaacgc
    12961 agcacaaaat tgtctttact tatttactgg ttgggttcaa ccttctctac tccgatacta
    13021 atatttgttt gtatacccct tctaaaatcg gtagtttcca ttcaattgat gggtactagt
    13081 tgattttaaa aactatgctt attcaatttt ttattttagc tcatatattt cgtttctttc
    13141 taaattttat actgatgaaa acaagtagca cacccaatat tgacaatata aactggctaa
    13201 tgatggtttc tccagttttt ggaagatgct tatttccact gcttgttgga ttgtttcctc
    13261 ttatatctga gctatcagca acttttctat tctcttttgt taaaaagatc gccacttctt
    13321 gttctttttc gatcttgaaa gttactggtg tcgcatccaa ttcatacccc gttggcgctt
    13381 gagtttctac cagctgatat gctcctggtg ctaagccatc tatcgccagt tttccatctg
    13441 ctcctgtggt tagacctgac tgcagaacgg ttccattggc atcttgtaac tcaaaaacag
    13501 ctccttgcag gacttctccg ctttggtcat cgatctttgt caacacgaca ccgcctggtg
    13561 taagtcgatt ttctttcgtc aattctacca ccgctgtctg acttctttcg atctcaaact
    13621 cgattggtgt cgcatccaat tcataccccg ttggcgcttg ggtttctacc agctgataca
    13681 ctcctggtgc taagccgtca atcgccagtt ttccatctgc tcctgtggtt agacctgact
    13741 gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg acttccccgc
    13801 tttggtcatc ga
//
LOCUS       DARBFD010000070        13810 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000070 DARBFD010000000
VERSION     DARBFD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.070"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(221..1423)
                     /locus_tag="SCK08_002013"
     CDS             complement(221..1423)
                     /locus_tag="SCK08_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1201873.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="SCK08_002014"
     CDS             complement(1426..1620)
                     /locus_tag="SCK08_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1201874.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="SCK08_002015"
     CDS             complement(1667..2515)
                     /locus_tag="SCK08_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEG1201875.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="SCK08_002016"
     CDS             complement(2720..3070)
                     /locus_tag="SCK08_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201876.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="SCK08_002017"
     CDS             3665..4111
                     /locus_tag="SCK08_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1201877.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="SCK08_002018"
     CDS             4735..5727
                     /locus_tag="SCK08_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1201878.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="SCK08_002019"
     CDS             5743..6627
                     /locus_tag="SCK08_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1201879.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="SCK08_002020"
     CDS             6676..8283
                     /locus_tag="SCK08_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1201880.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="SCK08_002021"
     CDS             8284..9768
                     /locus_tag="SCK08_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEG1201881.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="SCK08_002022"
     CDS             9945..10937
                     /locus_tag="SCK08_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1201882.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="SCK08_002023"
     CDS             10989..11858
                     /locus_tag="SCK08_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1201883.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SCK08_002024"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SCK08_002024"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1201884.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DARBFD010000071        13690 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000071 DARBFD010000000
VERSION     DARBFD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.071"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            139..477
                     /locus_tag="SCK08_002025"
     CDS             139..477
                     /locus_tag="SCK08_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201885.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="SCK08_002026"
     CDS             complement(682..1257)
                     /locus_tag="SCK08_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEG1201886.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="SCK08_002027"
     CDS             complement(1989..2570)
                     /locus_tag="SCK08_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1201887.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="SCK08_002028"
     CDS             complement(2753..3379)
                     /locus_tag="SCK08_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1201888.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="SCK08_002029"
     CDS             complement(3401..4732)
                     /locus_tag="SCK08_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEG1201889.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="SCK08_002030"
     CDS             complement(4887..5288)
                     /locus_tag="SCK08_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1201890.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="SCK08_002031"
     CDS             complement(5374..5580)
                     /locus_tag="SCK08_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201891.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="SCK08_002032"
     CDS             complement(5675..7177)
                     /locus_tag="SCK08_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEG1201892.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="SCK08_002033"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="SCK08_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1201893.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="SCK08_002034"
     CDS             complement(7748..8365)
                     /locus_tag="SCK08_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEG1201894.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="SCK08_002035"
     CDS             8586..9959
                     /locus_tag="SCK08_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1201895.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="SCK08_002036"
     CDS             10034..11146
                     /locus_tag="SCK08_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEG1201896.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="SCK08_002037"
     CDS             complement(11218..12855)
                     /locus_tag="SCK08_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEG1201897.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..13631)
                     /locus_tag="SCK08_002038"
     CDS             complement(13023..13631)
                     /locus_tag="SCK08_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1201898.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3690 a         2885 c         2452 g         4663 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    13621 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    13681 ttatcattta
//
LOCUS       DARBFD010000072        13429 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000072 DARBFD010000000
VERSION     DARBFD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.072"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..914)
                     /locus_tag="SCK08_002039"
     CDS             complement(<1..914)
                     /locus_tag="SCK08_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEG1201899.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="SCK08_002040"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="SCK08_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEG1201900.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="SCK08_002041"
     CDS             2006..2176
                     /locus_tag="SCK08_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201901.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="SCK08_002042"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="SCK08_002042"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEG1201902.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="SCK08_002043"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="SCK08_002043"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEG1201903.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="SCK08_002044"
     CDS             4440..5321
                     /locus_tag="SCK08_002044"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEG1201904.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="SCK08_002045"
     CDS             5318..6034
                     /locus_tag="SCK08_002045"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEG1201905.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="SCK08_002046"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="SCK08_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEG1201906.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="SCK08_002047"
     CDS             6751..7752
                     /locus_tag="SCK08_002047"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEG1201907.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="SCK08_002048"
     CDS             7749..9110
                     /locus_tag="SCK08_002048"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEG1201908.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="SCK08_002049"
     CDS             9070..10221
                     /locus_tag="SCK08_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEG1201909.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="SCK08_002050"
     CDS             10196..11365
                     /locus_tag="SCK08_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1201910.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="SCK08_002051"
     CDS             11540..12919
                     /locus_tag="SCK08_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1201911.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="SCK08_002052"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="SCK08_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DARBFD010000073        13408 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000073 DARBFD010000000
VERSION     DARBFD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.073"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            297..2036
                     /gene="yfcC"
                     /locus_tag="SCK08_002053"
     CDS             297..2036
                     /gene="yfcC"
                     /locus_tag="SCK08_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1201912.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2193..3377
                     /gene="iadA"
                     /locus_tag="SCK08_002054"
     CDS             2193..3377
                     /gene="iadA"
                     /locus_tag="SCK08_002054"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEG1201913.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3488..3691
                     /locus_tag="SCK08_002055"
     CDS             3488..3691
                     /locus_tag="SCK08_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1201914.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3792..4454)
                     /locus_tag="SCK08_002056"
     CDS             complement(3792..4454)
                     /locus_tag="SCK08_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1201915.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4467..5969)
                     /locus_tag="SCK08_002057"
     CDS             complement(4467..5969)
                     /locus_tag="SCK08_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1201916.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6517..8196
                     /locus_tag="SCK08_002058"
     CDS             6517..8196
                     /locus_tag="SCK08_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1201917.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8323..8802
                     /locus_tag="SCK08_002059"
     CDS             8323..8802
                     /locus_tag="SCK08_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEG1201918.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9021..9701
                     /locus_tag="SCK08_002060"
     CDS             9021..9701
                     /locus_tag="SCK08_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEG1201919.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9709..11040
                     /locus_tag="SCK08_002061"
     CDS             9709..11040
                     /locus_tag="SCK08_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1201920.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11055..12017
                     /locus_tag="SCK08_002062"
     CDS             11055..12017
                     /locus_tag="SCK08_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEG1201921.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12128..12466
                     /locus_tag="SCK08_002063"
     CDS             12128..12466
                     /locus_tag="SCK08_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEG1201922.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12545..13252
                     /locus_tag="SCK08_002064"
     CDS             12545..13252
                     /locus_tag="SCK08_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEG1201923.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4299 a         2348 c         2925 g         3836 t
ORIGIN      
        1 tatttttttt gcaatgaaat tcaatttttc aatgattgta aaatgaaaaa aacaggacga
       61 gattttctct aatctaaaaa taaaaaaaga attttaagga aaaacagaga aataaaatac
      121 ttaatataaa tggaaagaga aaaaaataat catacatagg agaaaaaaaa caaaatagaa
      181 aaaagtgatt ttctaataaa ttattcatgt ggaaatgtca tttttactgt ataattcatt
      241 tgattatcca ttttttattc atcaaaatta ctgaaaaaag aaaggaagaa ataaatttga
      301 agaaaacaaa ttcttcagga aaaccgaaac gcgacttcac taaattatcg acaccccata
      361 cgtatgtcat tatttttggt gtagtcatct ttgcatggat actgactttt gtcgttccag
      421 caggaaagtt cagcacgcaa gatattgaat acaaagatgc aaatggcgaa acaagtactc
      481 gtactgtttt acgtcaagat tccttccgtt atgcttacga gctggataag tcgtatgtgt
      541 ttgatcaatt agaagaactg caagatcatc cagcagaacg tgaaaaactg gatgttccgg
      601 aaaaaggatt ggaaaaagtc attgctgacg gagaaaaaaa tcttacgcag gaaaaactag
      661 atgaaatctc cttgacagat gatgtgcttt acgatcagta cggcgaaaat atctatgata
      721 cttctaaaaa attgcataaa acagccaaaa tatggggaac agatgatttt ggcggctttg
      781 gtttcttaaa ttttgttttt gaaggactag tatctggaga taagtatgga tctgctgtag
      841 ggatcgctgc attgatcctt gttgttggag gtgcttttgg tattattatg cgtaccggag
      901 cgatcgacgc aggtatctat gcgtttatca gtaaaactaa gggactggag cgtttagctt
      961 taccgctatt attctttgct ttttcattcg gtggcgctac attcgggatg gcagaagagg
     1021 taattccatt ctcaatggtg atggttccat ttgtcattgc gttaggatat gactcgatcg
     1081 tagcagtaac cgtgacttat gtcgcatctc aagtcggaaa tgcgacctct tggatgagcc
     1141 cgtttagcgt agcggtggct caagggattg ctggaatccc cgtgttgtct ggtgcgacat
     1201 tccgattgat tatgtgggta gttgttactg cattagccgc tggttatatg atgatatatg
     1261 cagaaaaaat ccgtaaaaaa ccagaaagtt cgttgactta caaatcagat gattatttcc
     1321 gttctcatat caaaaagaca tctgatgaaa ataaaaaatt catgcttggg cataaattga
     1381 tcttattaga gatgttagtt gtccttgtct ggatcgtttg gggcgtgact caaaaaggct
     1441 attacattcc agaaatcgct tctcaattct ttgtgatggg attagttgct ggaatcattg
     1501 ctgttctttt caaattagat ggaatgaaag tcaatgacat cgcttcttct ttccaatctg
     1561 gtgcagctga tttagctggt acagcaatcg tagtaggtat ggccaaaggg attcttcttg
     1621 tcttgggagg atctgatgca tctgttcctt cagctttgaa tacgattttg catgggatcg
     1681 ggacggcgtt gaccggtgtt ccagctgtca ttggtgcttg ggcgatgtac atcttccaaa
     1741 gtttgttcaa cttggttgtt acatcaaatt caggacaagc agctttgact atgccaatca
     1801 tggctccact ggcagattta gtaggtgtat ccagacaagt agcagttctt gcttatcagc
     1861 ttggttcagg ttttatggac gcttttacac ccgtatcagc cagcttgatt ggtgtgctag
     1921 gtgttgcacg gatcgaatgg gcaaaatggg caagattcca aatcaagatg caaggattcc
     1981 tgtttgtttt aggaactatt ttcatcatga ttgccattgc aataggtctg cagtagtact
     2041 ggagacctgt aaataaaaaa gtaatccaaa cagtagataa ggactccaag aaccgtcatt
     2101 ttacggtaaa atcaaaggag taaagatcct atcgtttgga ttatttttta agatcctatc
     2161 atcagttata cttaaagaaa gaggaaaaat gtatgaaaat cattcgacaa attgaagtgt
     2221 ttgcaccaga ttacttagga aaaatggatg tactgattgc cggtgggaag attttggctg
     2281 tagaagaaca gctagaaggc ggctacgaag gagtggaagt agaagaactt tcaggtgagg
     2341 gaaaagtact aaccccgggt ttcattgact gtcatttcca tattttaggt ggaggaggag
     2401 aaggcggtta tcaaaatcga acgccagaag tcacattgag ccaattgact acagctggtg
     2461 taacgacagt tgtcggttgt ttgggaacag acggagaagg tagagatatg actgcgttga
     2521 tcagcaaagc gaaaggtctg gaagcagaag gaatctccac ttatgtatac gaaggttcgt
     2581 accgattgcc ggtaaaaaca gtaacagatt ctatcatcaa agactttttg acaattgata
     2641 aaattatcgg aatcggagaa atcgcagtat ccgatcaccg ttcttctcag ccaagttttg
     2701 aagagttcgc tcgtgcagta gcagatgctc gtgtaggagg catgttatcc ggaaaagctg
     2761 ggatcatcaa tgtccactta ggcggaggaa aaagaaagct agagctattg acacgaatcg
     2821 tagaagaaac agaaattccg atcacacaat ttttgcctac tcatgcaaat cgcacaccag
     2881 aattatttga agcttgcgtg gcatttgcca aacgtggtgg cacgattgac ttcactgcta
     2941 gcgaagatcc tgatttttgg gagaagacag atggtgaagt tcgcttcagc aaagcactga
     3001 aacgattgat cgaggaagat gtttctttag ataatttcac gatgacctca gatggacaag
     3061 gaagcttgcc ttattttgat gaaaacaatc atttcctagg gttaggagta ggaagtgcca
     3121 aggcattgct agtagggatc aaagaagctg tgcaaaaaga aagtatccct ttggaaatcg
     3181 ccttacgagc tataaccagt aatccagcac gtatattgaa attggataaa aaaggaaaaa
     3241 tcgaaattgg tgctgatgca gatctgtgta tcttggataa agaaacgctt gatattgata
     3301 cagttatcgc taaaggagaa ataatggtcc aagaaaaaga agtcaaagta tgggggactt
     3361 tcgaaaaaag tttttaatca acagctctaa ctatcttttt tagaaaacga gcgttctgtt
     3421 tgtctgtatc tgttgttgat gttaccctga aggtagatca ataaacaaac taagaggagg
     3481 aagcacaatg gcagatttaa aaggacgttc taaagatatg aaagataaag tagtcggtga
     3541 aggaaaagaa gcatacggca aagcaacaga cgacaagagc aaagaaacag aagggaaagc
     3601 acaatcacta ggtgctgaca tcaaagaaaa agctcgaaat ttaggcgacg aagtcaaaga
     3661 caagttttct cgaaaagacg aaaaaaaata aaaaccactt attactaatt gaatcaagca
     3721 aataaaaaat ccgaaccata tctgcgagac agatatggtt cggatttttt atttgatcat
     3781 tatcctgttt tttaaacttc gattttttga aattttttat tttttagttc aaagacttca
     3841 tcacagcttt ttgctacttc aatctcatga gtcacgatga taatacagcg attttgttca
     3901 tgggcgattt cttggaaaag tttgacgata tcttgtgaag ttgtttcatc tagatttcca
     3961 gtaggctcat ccgctacgat tagttgatgt tcacagcaga tagcacgaac gatagcgact
     4021 cgctgctgct gacctcccga aagtttggtc acctgttttt ttgctaatat ttcatcaatt
     4081 cctacacgag ccagtcgatc taaagcaact tgtcgataat tctcttgctt ggtcttagtg
     4141 atttccattg ctgctatgac gttgtctaga gccgataaat aaggaagcaa gttgtaagct
     4201 tggaaaataa tcgagacatc atttttacga taacttctta acccgatttt gttcagtgaa
     4261 cgatcttgat agtatacctt tccttctttt ggtgcatcta gtccagaaat aagcgaaaga
     4321 aaagtcgttt ttccagaacc acttgctcct aaaatagcgt acatttttcc agcttcgaaa
     4381 ttcagttcta catctttata aagcgcatct tgcggatctt gataccaaaa tcccaattgt
     4441 tctgtccgta acatcaaatt cctcctttag tttaatagga tttttcttgg gtttaatctt
     4501 aatatcccta tagaagctaa aaagacagaa aacagactga ttccaaatgc tactccggct
     4561 aatagtccca attgtgcagg tttgacattg atttccaatt cgctgacttg atcagaaaca
     4621 gcaaatggat tattcgtggt tccttgttga ccatcgcgtt gaccgccttg ttcttgtctg
     4681 ttacctggca tttgtccacc gccaggagct tgctggtttt gtgtcgtggt ttctgtctgt
     4741 tggtcaagta gctgctgtcc cacggcattc gcgacgacgt ttccgctaag cgaagcaact
     4801 cctaatgcaa gaatcataca aatagccact tcggtaaaca attggagaat gattttactt
     4861 ctggattctc ctaaagatag caagacgcct aactcatgcc gacgctcccg aatggtaatc
     4921 ataatgatca gagtaagtat gataataccg gctacagcca ctagtaaaac aacattttta
     4981 gaaaaacttg ccacgttgtt taaaggttcc aacattgact gatacatgga atcatttgat
     5041 tgtaagctaa atgtatctgt gtcgatcagc ttctcagctt ctttgagaaa ctcatccatt
     5101 ttatctggat cagatagagt gtaaatagca gaatctatcg tgttatcgtc agatgtccca
     5161 tttagttcat ttgcaaaagt gtaagaagtg taaagtgtat tcgacggatt catgaagttg
     5221 aatttcatac ccatactgcc gcttgtttcg ctggattcat aaattccttt gatcgtcatt
     5281 tcataagtgg tatcttcatc ttcagtactc gtgattgtga aagtatctcc gactgccagg
     5341 tcatttgttt ctgctagagt agaatcaatg actacattgt ttgtgccttt gtcgctgtca
     5401 tctattcctt caccatccgt gatttttgct gttccatcag aaaaattact attttgtgca
     5461 gttgctgaca caccactgat ttgaaagtct gcttgagtca tttgcggatt cccgcccatt
     5521 cctccagcag gttcaccgcc tgcatcagta gtttctgaac tgtcttcact tgaatcagaa
     5581 ctagtgatgg cagttattcc atcttttgcc atagctgagg cggaactttc aaaggaataa
     5641 ctctttacat tatctaattc agctattttt tccgcgtccg ttaaggaaac tggtgtaaga
     5701 gaaaaactgc ctggatctgg acgttcttca gtggtagaac cgcttgaaga agtttgttcc
     5761 tgttttttaa atgaagcttc tcgattggca cttaaagtca ctgttgctcc aacttctttt
     5821 tgtgcttgat tcgctgcttc ttctgcagcg cttcgaatcg tgaggccagc tagcacaaaa
     5881 ataagaatgg ctgtgaacac ggcaaacaat aacaatgaac gtccccattt tacttttgta
     5941 cttttccatg cacgtttcat aaaattcata ttttgcatcc ctcctagtag atgcatttga
     6001 taatagacaa cctatcttaa agctagctta aatcttatgc tgtttgcaca ccatcaacat
     6061 gaggttctgt atatataaaa agtcactata gttattgcaa aaaatgtggt aaaatgaaaa
     6121 aagtatgttt caaaccgacg aagaagaaat gaaagaggaa tagcaaaaga aatcctagat
     6181 gctacagagg gatttttacg tgcatagttg ttctaacaat tgctgatact agcgatttag
     6241 tttatttgtt gtataataat ggggaacagc tgaatactta tataagtatt ctggctatag
     6301 cgtttcgcaa aagatccatt ttgttaatga agtatatcca aaataaaatg cccatccaac
     6361 tgtcagaacg cggatgtgct ggtaattttc ctgctgattc tccataagtt gagaaatagt
     6421 gattttgcat atgcttcatt ggaatggctc atatatttag ataaagaaga aaatcgacat
     6481 ctttaaactt ggatgtcata agaagaggtg aagtttgtga gtaccattaa aattatccca
     6541 ttaggcggcg ttcgtgaaaa tggaaaaaac atgtatgtcg cagaagtggg cgaggatatt
     6601 tttattttag attgtgggct aaaatatcct gaaaatgagt tgttggggat cgacgtcgta
     6661 atccctgatt ttacgtattt agaagaaaac attgatcgtg tagctggtgt ctttttaaca
     6721 catggacatg cagatgcgat cggggcactt ccttacttgt tgtcaaaaat ttctgtacca
     6781 gtattcggga cagagttgac gatcgagtta gcaaaattga atgtcagtcg taatgaagca
     6841 gccaaaggat ttaaggattt ccatgtggta gatgagcata cagaaataga ctttggtgaa
     6901 acagtagtga gctttttccg taccactcat acgataccag attcagtggg gatcaatctg
     6961 aaaacaaaag aaggtagtat cgtttatacg ggagatttta aatttgacca aacggctatc
     7021 ccaatgtatc aaacagatta tgcacgattg gcggaaatcg gcaaagaagg agtcttagcc
     7081 ttactaagtg aatcggcaaa tgcagaaaat cctcagcctg tagcttctga agcccaaata
     7141 gcagatgaag tatttgatac gatcaaatat tgggaagggc gtatcatcgt cgcttgtgta
     7201 gcaagtaatt tgcaacgagt gcaacaagta ttggatgcag cagaaaaagc acaacgaaaa
     7261 gttgtcttga ctggtcaaga tttcgaacga attattcgca cagcgatgcg tttagaaaaa
     7321 cttcaattac caagtgaaga tctgcttgtt acgttgaaag acatgaaaaa ctatgatcca
     7381 gaagaactga tcattttaga aactggccga atgggtgaac ccatcaagtc tctgcaaaaa
     7441 atggcaaacg gtacacaccg taatgtcaag attgaagaag gagatttggt ttatatcact
     7501 actacgccaa gcatcgcgat ggaaaccatc gtggcgaaaa cggaagatat catttatcga
     7561 gctggtggaa cagtgaagtt gatctctgag aatatgcgcg tttctggcca tgccaatcct
     7621 aatgacttgc aattaatgat caatttgatg aaaccaacat attttgtgcc agttcaagga
     7681 gagtatcgtg aattagctgc tcatgcagat ttggctcatg cagtagggat gccatacaaa
     7741 aatatctata ttactggacg cggagacgtg ctggaatata agaacaagcg tatgagtgtt
     7801 gcaggaacta caagtgcaga aaatgtaatg attgatggat taggcatagg tgatatcgga
     7861 aacattgttt tacgtgatcg aaaaatcttg tcagaagacg ggattttcgt agcggttgtc
     7921 actatcagtc ggcgagaaaa gaaaatcatt tcaaaacctc aaattacttc acggggattc
     7981 gtttacgtaa aagctagccg tgatctgatg aaagaaagca gcaatatgat tgaaacaatc
     8041 gttgagaaac atttagaaag caatgatttt gagtggagca aattaaagca agacatccga
     8101 gatcaattag gccgttactt atttgaacaa acaaaacgcc gcccagtgat cttgccagtc
     8161 attatggaag ctactcaacg tcgaaataaa aaataaacaa aaagagtaag atgaacctct
     8221 ctatagagtg ctcgtcttgc tctttttttg cgaaaaacaa ataatttttg aatacagcat
     8281 ccctcgcttt ataataaacg atagaataaa gggagtgaat caatgatgag tcaaacaaaa
     8341 gtcgaacgtt atttagaaga acaaaagatc agctatcaac cacttgattt tagtcatgtg
     8401 gtagaagaat ctttcacaga agaactacaa aaaagaggga ttgatccagc tttgatatgt
     8461 aaaactctcg tagtgaaagg aaacaaaact ggggtgatca tcgcattgat tcctttgaac
     8521 gatcacttag attacaaaaa aacgagacaa gtgacgaaag atcgtaaagt tggttttcct
     8581 ggaatggatt ttgtattaat gcatacagga tatccccacg gagcaaacac ccctattgga
     8641 ataaaactcg cacatcctga ttattgtttt ttagcagaca gttctttaaa agaaaaagaa
     8701 gaagtaattg tgtcaagcgg tgaaatcggg agaagccttc ggataaaagt agccgattta
     8761 gaaaagatcg tccatcctat ttacgctgat ttaattagtt aagggacgag aagagaagct
     8821 aagcagagca gagacctcaa acttgagatt atatctgctt ggtacttggt gcatcctgta
     8881 ttatctgtta caattgcttt gtacatactg tactatcaat ccaatgaaaa tatatttagc
     8941 agctatattt tttcattgat gtgcaagtga tgaaatcgtt tttaataaaa aagatacaga
     9001 gaaaacgcga ggtgtaaaat atgaaaatta cgtatcatgg acattcctgt ttaacaattg
     9061 aaatggaaaa tggccaacgc gtgatgattg acccgtttat tacaggaaat ccattgacag
     9121 acttaactgc agaagaagta aagacagatt ggttgctgat tactcatggt catagtgacc
     9181 acataggaga tatgattcct attgcaaaag aaaatcaagc cacaattatc agtattgttg
     9241 agattgctga ttatgcgaac agcagaggag tagattcatt cggtatgaat atcggtggaa
     9301 aacatgcgtt tccattcggc gtggtaaaat tcgtacatgc acagcatagt tcaagctatg
     9361 aagtagacgg tatcgttcag tatatgggcg agcctagcgg aattattatt caagcagaag
     9421 gaaaaacgat ttatcatgca ggggatactg cttatttcag tgatctagga ttgcttgcag
     9481 aagaattcga tattgatgtc gctttcttgc cgattggcga taactatacg atggggccag
     9541 aagatgcagc aaaggctgcg caaacaattc gtgccaagaa agtagttcca atccattaca
     9601 atactttccc tgtcattgaa caagatcccg aatcttttgt agagttactg gaagatagtg
     9661 aaggaaaaat aatgagcgtc tcggaaacgc tggaagtgta ggcagaaaat ggcgacaaaa
     9721 catgatttga ttttagaata tattgaaagt ttacctgttg gcaatcgaat ttctgtaaga
     9781 agcatcgcaa aagaattgaa tgtcagtgaa ggaacagcgt accgtgcaat caaagatgca
     9841 gaaaatgtcg gactagtttc aacaattcaa cgagtaggga ccatccgtat cgaacgcaaa
     9901 ttaaaaaaac atattgaacg tttgacattt ggcgaggtcg tcaggatcat tgaaggggac
     9961 gttttaggcg gggcggcagg attagacaag gtcttgaata aattcgtcat cggcgcgatg
    10021 actaaacatg ccatggaacg ctatattacg ccaggatcac taatgatcgt cggcaaccgg
    10081 gcggaagtcc aaaaattagc attggaagat ggcgccgcag tattgatcac aggaggattc
    10141 aatacgactg ctgagattgc aaagctagct gatgatcttg aaatgccgat cttacgcacc
    10201 tcttatgata catttacagt cgcaaccatg atcaaccgag cgttaagcga ccaattgatc
    10261 aaaaaagata tcatgcttgt cagtgatatc tatatgccag ctgaaaagac tcattatttg
    10321 cagcagacag atacgatttc tgattatcag cgtttgtcag aagaaacgca gcattcgcgt
    10381 tttccagtcg tcaaccgcca tcatcgtttg atggggattg ttacggcaaa agatgtttta
    10441 gggaaatcgc caaaccagat catcgatcgg gtgatgacga aagaaccaat cagtgtaaag
    10501 aaaacaatga gtattgcctc tgttagccat caaatgatct gggatgggct agaagtcatg
    10561 ccggtcgttt ccgatgactt gacattggaa ggcttgatta cccggcaaga tgtaatgaaa
    10621 gcgatgcagc ttgtccagcg acaaccgcag attgcagata cgatttcgga tcagatatct
    10681 ggaaatatcc atacgattga tactgaccgt gaaggaaatc gtttggaaaa ccctaagttc
    10741 aaattcgtag tagcaccaca aatggtcaat ggcgttggaa ccatttcttt tggcgtactt
    10801 agcgaaatca tttctgatgt cgcacaaaaa acaatggtga tgaaccaaaa acgaaatatc
    10861 ttgattgaac aagtgaacct acattatttg cggctgatcc aattggaaag cgaattagac
    10921 attcgtccac gtgtattgga aatcggcaga cgttccgcta aactggatat cgaagtgttt
    10981 attgaaaaca ccatcgtttc caaagcaatc gttgtttgtc aagtaatgga acgttcttag
    11041 aaagcaggag tgaaatgaca gtacagcaag aaatagtaga tacaataaaa aaatacgata
    11101 aaatcattat ccacaggcat ttgcgccctg atcctgatgc attaggatca caagtaggat
    11161 tagccgaact attacgggaa agtttcccag aaaaagaaat acgtcaagta ggtgaaacga
    11221 ttgaagggct tcgcttttta gcagaaatgc aagaagttga agatgtcttc taccaaggag
    11281 cgctagtcat cgtgactgat acagctaatt cacccagaat cagtgatcaa agatatcagt
    11341 tggcagaaaa actgatcaaa atcgaccatc atcccaatga tgacccttat ggagatcttg
    11401 tgtgggtaaa tacaaaagca agtagctgta gtgagatgat tgcagaattt gctttgatgt
    11461 ttcctgaatc tttaaagatg aacgttaagg ctgcacggtt actttatgca ggaatcgtcg
    11521 gagatacagg gcgctttttg tatccctcca cgacgtcccg tacattagag attgcttcga
    11581 tattgagaaa ttatccattt gatgcaagtg ctttgaacag agagattgaa caaatgccga
    11641 tgaaagtcgc taaactttcc ggttatcttt atcaaaatat ccaggtggat gaaaatggtg
    11701 caggaagaat aatccttccg caagaaattc ttgataaata tggaatcgat gattcagaga
    11761 cagcttcgat cgtttcactt cctggagtga ttgacgaagt gctggcttgg gggatttttg
    11821 ttcagcagcc ttcagggtat taccgcgtac gtttacgttc taaggggccg atcatcaatg
    11881 aattggctaa acgccatcat ggaggaggcc atccattagc aagtggtgcg aatgcaaaag
    11941 atttagcaga agtagaggaa atctataaag agatccaaga tatatgccaa tcgtataaga
    12001 gcagaaaaac agaatgatac ttcgttcgaa aacgtgcctt ttgtagtaaa aatcacaaat
    12061 gtatcttttc aagatcttct ttttccgtta caatagtgct cactaaagat tttaaaggag
    12121 aaagaagatg acattaccaa attgcccaga atgtggctca gagtacacat atgaagatcg
    12181 aggcttgttg atttgcccag aatgcgggaa cgaatggaac aaagaagaac aagaaactgt
    12241 catagaagac ggattagtgg tattagatgc aaatggaaac cgtctgttag agggcgatag
    12301 cgtgacagtg attaaagact tgaaagtaaa aggtgcgtca ggtgccatta aacaaggaac
    12361 aaaagtcaag aacatccgtt tagtagaagg cgatcataat atcgattgca aggtagaagg
    12421 atttggaccg atgaaactga aatcggaatt tgtgaaaaag aattaaagaa tattcaaggt
    12481 cttttcacat tcttttccta ctactaatga taaaataaag taaacgaaaa ggagtgaaac
    12541 gaaaatgtat ggttatggaa tgtatggttt tttagatccg acatttcttt tggtcattct
    12601 tggattagtt atttccggaa ttgcgtcagc gtacgtcaat agtactttcc gaaaatacga
    12661 tcaagtgaga agcaaaaaca atgtgactgg tacacaagca gcacaatata tattacaaag
    12721 tcaacagata aataatgtcg gtgttcaaca aattgccgga gatctcacag ataactacaa
    12781 ttcaggtaat aagatgttga gtttgtctca agcaactgca cagtctactt cagttgctgc
    12841 gataggtgta gcagctcatg aatgcggaca tgcggtacaa gatgcttcgg gttatgtccc
    12901 attgaagatt cgtgcagcac ttgttccagc agctaacttt ggttcgatga tttcgttccc
    12961 cttgattatg gtaggtgtat tgcttagctg gaactctacg ttaatcaata tcgggatttt
    13021 cgctttttct ttagcattac ttttccaatt ggtgacatta ccagtcgaat ttaacgcgtc
    13081 tcggcgagca atccaaattt tgagtgaagg cggactactg acggaagaag aagtaccgat
    13141 ggcaagacac gtattatttg cagcagcact gacttatgtt gcagcagcgc tttcgacatt
    13201 cctgcagctg ttgcgcttga tcttattgtt cggcggcaac cgaagagatt aaaataaaga
    13261 agttgtgaaa tcagcgcttt gatctgagct aataccgaga gttagctgat tgaataccat
    13321 ttatttggaa taagggctct ttgtcaataa ggactgatgg gttgtacaaa atcaaatctg
    13381 ggtcagaatg aacctcattc tggctcag
//
LOCUS       DARBFD010000074        13404 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000074 DARBFD010000000
VERSION     DARBFD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.074"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            79..1125
                     /locus_tag="SCK08_002065"
     CDS             79..1125
                     /locus_tag="SCK08_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG1201924.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="SCK08_002066"
     CDS             1474..2106
                     /locus_tag="SCK08_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEG1201925.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="SCK08_002067"
     CDS             2694..4133
                     /locus_tag="SCK08_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1201926.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="SCK08_002068"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="SCK08_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="SCK08_002069"
     CDS             4895..5110
                     /locus_tag="SCK08_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201927.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="SCK08_002070"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="SCK08_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5770..6681)
                     /locus_tag="SCK08_002071"
     CDS             complement(5770..6681)
                     /locus_tag="SCK08_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1201928.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="SCK08_002072"
     CDS             complement(6700..7704)
                     /locus_tag="SCK08_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1201929.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="SCK08_002073"
     CDS             complement(7701..9824)
                     /locus_tag="SCK08_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1201930.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="SCK08_002074"
     CDS             complement(9829..12276)
                     /locus_tag="SCK08_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1201931.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="SCK08_002075"
     CDS             complement(12263..12652)
                     /locus_tag="SCK08_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1201932.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="SCK08_002076"
     CDS             complement(12712..13215)
                     /locus_tag="SCK08_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1201933.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13389)
                     /locus_tag="SCK08_002077"
     CDS             complement(13228..13389)
                     /locus_tag="SCK08_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201934.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4099 a         2642 c         2454 g         4209 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attg
//
LOCUS       DARBFD010000075        13395 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000075 DARBFD010000000
VERSION     DARBFD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.075"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(14..706)
                     /locus_tag="SCK08_002078"
     CDS             complement(14..706)
                     /locus_tag="SCK08_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1201935.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="SCK08_002079"
     CDS             complement(1143..1712)
                     /locus_tag="SCK08_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1201936.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="SCK08_002080"
     CDS             complement(1786..2109)
                     /locus_tag="SCK08_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201937.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="SCK08_002081"
     CDS             complement(2263..3075)
                     /locus_tag="SCK08_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201938.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="SCK08_002082"
     CDS             3429..4277
                     /locus_tag="SCK08_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEG1201939.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="SCK08_002083"
     CDS             4422..5363
                     /locus_tag="SCK08_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEG1201940.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="SCK08_002084"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="SCK08_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="SCK08_002085"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="SCK08_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEG1201941.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="SCK08_002086"
     CDS             complement(9827..12013)
                     /locus_tag="SCK08_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEG1201942.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="SCK08_002087"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="SCK08_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEG1201943.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="SCK08_002088"
     CDS             complement(12235..12675)
                     /locus_tag="SCK08_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEG1201944.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="SCK08_002089"
     CDS             complement(12749..13288)
                     /locus_tag="SCK08_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1201945.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3763 a         2807 c         2380 g         4445 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaa
//
LOCUS       DARBFD010000076        13270 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000076 DARBFD010000000
VERSION     DARBFD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.076"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            166..813
                     /locus_tag="SCK08_002090"
     CDS             166..813
                     /locus_tag="SCK08_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1201946.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    942..1158
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1206..3851
                     /locus_tag="SCK08_002091"
     CDS             1206..3851
                     /locus_tag="SCK08_002091"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEG1201947.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4161..5474
                     /locus_tag="SCK08_002092"
     CDS             4161..5474
                     /locus_tag="SCK08_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEG1201948.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5464..6117
                     /locus_tag="SCK08_002093"
     CDS             5464..6117
                     /locus_tag="SCK08_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1201949.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6172..6723
                     /gene="radC"
                     /locus_tag="SCK08_002094"
                     /pseudo
     CDS             6172..6723
                     /gene="radC"
                     /locus_tag="SCK08_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6854..7939
                     /locus_tag="SCK08_002095"
     CDS             6854..7939
                     /locus_tag="SCK08_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1201950.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7936..9828
                     /locus_tag="SCK08_002096"
     CDS             7936..9828
                     /locus_tag="SCK08_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1201951.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9835..10212
                     /locus_tag="SCK08_002097"
     CDS             9835..10212
                     /locus_tag="SCK08_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1201952.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10366..11148
                     /locus_tag="SCK08_002098"
     CDS             10366..11148
                     /locus_tag="SCK08_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG1201953.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11274..12005)
                     /gene="erm(A)"
                     /locus_tag="SCK08_002099"
     CDS             complement(11274..12005)
                     /gene="erm(A)"
                     /locus_tag="SCK08_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG1201954.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(12063..12122)
                     /locus_tag="SCK08_002100"
     CDS             complement(12063..12122)
                     /locus_tag="SCK08_002100"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG1201955.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12514..13176
                     /locus_tag="SCK08_002101"
     CDS             12514..13176
                     /locus_tag="SCK08_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1201956.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         4557 a         2037 c         2729 g         3947 t
ORIGIN      
        1 atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag cttttgaaca
       61 ccgttttatt tgtctcacac ccttttcctt cctacttgta accttgctcc cgtgttagaa
      121 acatgttaca ctaaatatgt gaaattatta acaaggagcg tcgttatgcg agaaaaaaca
      181 gatacactgc caaaagcaac tgcgaaaaga ttacctttat atttacgcta cttgaagatg
      241 ctggatgact caggtataag tagaatcaaa tcaaatgagt tcagcgaaat cacccaaatt
      301 ccttctgcaa cgatccgcag agacttctcg caattaggcg agctaggcag aagcggatat
      361 ggctatgatg tcccttttct tatcgatgta ttcaataata tccttaatac taaagaagaa
      421 aaacgaatcg cattagtagg atatggaaat cttggcaaag cattgaaaca caacaatttt
      481 agaagaaatg aaaatctaaa tatcgtgtgc gtctttgata atgatccagc actgatcaat
      541 cgagtaattg atggagaaat gatctatccg attgatcgtt tcgcggagat agccaaagca
      601 aaaaatgtga cagtagcaat ctctacggta ccaagtaaat attcacaatc tgctattgat
      661 gaaattgtca aaggaaatgt aaccgctata cttaattttg cgcctgacag agtaactgtc
      721 ccagcttatg tcaatgttca atacattgat ttgacaaccg agttgcagac gcttatttac
      781 tttgatgaga attactcaga agttttttct taggaaaatt tgagtaggct tattgatctt
      841 gcgagaactc gtcgtaattc tttcaaaaat tttttagtac taaatgaagt ggttttttca
      901 cgaccacttg ttgactaaaa aaaaaaacca ttgtagaatg aagcataacg ctgaacaaga
      961 ggagtaactg gcacattcat tcttagagag agaactatct gctgaaaggt tctcaatgac
     1021 aaccagtgaa ggtagcttgg gagtttctgt cttgaatgga gtagagatag acgagtcgtg
     1081 atcgttaact cacttgagag gcaataatga ttgattattg caatttaggt ggtaccgcgt
     1141 aaatcatacg tcctagaata cgaaagtatt ttaggacgtt ttttagttta aaggaggaaa
     1201 acagcatgac ggaagaaaat aacctatcaa caaaatataa tccgcaagaa gttgaagctg
     1261 gacgttatca gcattggtta gaacaagatt tgtttaaacc gagcggagat aaaaaagcaa
     1321 aaccttattc aatcgtaatc ccgcccccaa atgttactgg aaaacttcat ttagggcatg
     1381 cttgggatac gaccttgcaa gatatgctga ttcgtcaaaa gagaatgcaa ggatatgata
     1441 ctttatggct gcctggtatg gaccatgcag gaatcgcgac acaagcaaaa gtagaagcga
     1501 agcttagaga gcaagggatt tctcgttatg atctaggtcg tgaaaagttc atcgatcaag
     1561 tgtgggaatg gaaagaagag tatgcgagcc acatccgcga acagtgggct aaaatgggct
     1621 tatctttaga ttacagtagg gaacgtttta cattagatga tggattatct gaagcagtac
     1681 gtaaagtttt tgttacctta tacgaaaaag gtttgattta tcgtggtgaa tatatcatca
     1741 actgggatcc tcaagcacga acagctctat ctgatatcga agttattcat aaagatatcg
     1801 aaggcgcctt ttatcatatg agctatgaac tagcagatgg atcaggcgta gtagaaattg
     1861 cgacaacccg tccagaaacg atgttgggag atacggcgat tgctgttcat ccagaagacg
     1921 agcgttatca agcgctcatc ggcaaaaaag tgattctacc tttagtcaat aaacaaatcc
     1981 caatcattgc agatgaatat gtagatatgg aattcgggac aggtgtagta aaaatcactc
     2041 cagcacatga tccaaacgac tttgaggtag gtaatcgtca tgatcttcca cgcgtaaatg
     2101 ttatggatga tgatggtacg atgaatgaac tagcaggaaa atatgctgga atggaacgtt
     2161 ttgctgctcg taaagcaatc gtgcaagatc tgaaagaaat cggtcgattg attaaaatcg
     2221 atacgatgat ccacagtgtg ggtcattccg agcggacagg tgtcgttgtt gaacctcggc
     2281 tgtctactca atggtttgta aaaatggcgc cgttagcgaa aaaagccatt gataaccaag
     2341 aaacagatca agcagtggaa ttcttccctc caagattcaa tcaaacgttt ttacgttgga
     2401 tggaaaatgt ccatgattgg gtaatctcac gacaattatg gtggggacat caaattcctg
     2461 catggtatca caaagaaaca ggtgaaatgt atgtagggat ggaagcacca gcggatagtg
     2521 aaaactgggt acaagatcct gatgttttag acacttggtt tagttcagct ttgtggcctt
     2581 tctcaacaat gggctggcca gatacggaaa gcgaagatta taagcgatac ttccctacta
     2641 gcactttagt aaccggttat gacatcattt tcttctgggt aagcagaatg attttccaaa
     2701 gcttggaatt tactggcgaa cgtccatttg agaatgtctt gattcatgga ttgattcgtg
     2761 acgaacaagg gcggaagatg agtaaatcat taggtaatgg aatcgatccg atggaagtaa
     2821 tcgaaaagta tggagcagat gctctccgct ggttcttatc gaatggttct gcaccaggac
     2881 aagatgtccg cttcagctat gaaaaaatgg atgcttcttg gaactttatc aacaaaatct
     2941 ggaatgcttc acgtttcgtt atcatgaata tcgaaggaat gaccgtagaa gagatcgatt
     3001 tatctggaga aaaatcagtg gctgaccggt ggatccttac tcgtttgaat gaaacgatcg
     3061 aacgagtaac tgagctattt gatcgttttg aatttggtga agcaggtcga caactgtata
     3121 actttatctg ggatgatttt tgtgactggt acatcgaaat gagtaaagaa gtactttatg
     3181 gagatgatgc tgtttcaaaa caaaccaccc gtagcatctt agtccataca cttgatcaga
     3241 tcctacgttt attgcatcca atcatgccat tcgttacaga agaaatatgg gaacatattc
     3301 ctcatcaggg aaattctcta gtagtggcag aatatcctgt tgtacgtcca gagtttgatg
     3361 atgaaacagc ttctaaaggg atggaagtat tgaaagaatt gattcgagcc gttcgaaata
     3421 ttcgttcaga agtgaataca ccattgtcta aaccaattac gttgctaatt aaaacaaacg
     3481 atgctaaaat cgatcaattt ttagttgaaa atacaagcta tatcgaacgc ttctgtaatc
     3541 ccgaagagtt gacgatatca agtgagatca ctgcgccaga tctagcgatg tcagctgttc
     3601 tgacaggtgc agaaatcttc ttgccgttag caggattgat caatattgaa gaagaaatca
     3661 aacgattaga aaaagaactg gaaaagtgga caagcgaagt aaaacgtgtc caaggaaaac
     3721 tttctaatga acgttttgtt tctaacgcgc cggaagaagt agtagaggca gagcgtgcaa
     3781 aagaaaaaga ttatttagag aagcaaacgg ctgtcaaaga acgaatcgaa agtttgcgta
     3841 ctattcaata agaatagaaa aagagagtgt gaaatcagct agctattact aaaagctagc
     3901 tgattgaaca tcgttacagg aaagagtttg agcctcactc ttagcttcga ttcataagga
     3961 gatgtgacat aaattttgtc gcatctcttt tttagtctac tcttattatg taggagaagg
     4021 agggaggtgg atcatgtatc aagagtcaat aaagtagttg ataaaatcgt tcctctctct
     4081 gtttcttttc tctttgtcat acttttattt ttcttatgtg ttctatttgt tataataaaa
     4141 gagaaatggg ggaattgttg atgacgattg aagaagcaat agaatggatc catagtcgat
     4201 taccatttgg ttcacgtcct ggccttgacc gagtcgaagc tttattagaa gtggtagggc
     4261 atccagagag aaaagtaaaa acgatccata tcgctggaac aaacggaaaa ggttctactg
     4321 tagcttattt gagaagccta ttagaagagg ctgggctgac agtaggtaca tttacttcac
     4381 cttatatcga aacgttcaat gaacggatcg caatcaatgg caaaagcatt ccagacaatg
     4441 atttgatcca gcttgtaaaa aaatatcagc cactgatcaa acaacttgat caatttgaag
     4501 cagtagctgg tattactgaa ttcgagacat taacagcaat ggcatttgat tattttttga
     4561 tgaagaaagt ggacgtagcg attatcgaag ttggcttagg cggcttattg gattctacca
     4621 atgtagctgt accaatgctg acgggaataa caacgattgg acttgatcat accgaaatac
     4681 ttggagatac aattgaagaa atcgctgcac aaaaagctgg aatcatcaaa caaggcgttc
     4741 cagtagtaac aggaaatatt gccccagaag cttttactgt catccaacaa acagctaaag
     4801 caaaaaatgc accgctttat gtatacgggg aaaattataa aataaactat ctgcatcctg
     4861 atgagatgtg gggagaagta tttgaatttt ttaatgaaat aggaaagatc cctcaattaa
     4921 ccactccgct tttagggcaa catcaagctg aaaatgcagg agtagccatc gaattgtatt
     4981 ttgtttactg tcaattggaa aagctgcctt tccgagaaaa agatgttttg aagggattga
     5041 agaaatctcg atggccagga agaatggagc gtttgagtaa cgagccatta gttgttttag
     5101 atggtgccca taatacgcat gcaatgaacc ggcttgttga gaatttgaaa aaagaattca
     5161 aaggatatca aatcaacatt ttgttttccg ccttacgaac taaaaatgtt agagaaatgc
     5221 tagaacgatt gcagcaagta ccaaatgtcc atatttattt gacgaccttt gaacatccaa
     5281 aagccatcga attaagtgaa gtatcagatt tagcaagtga gagagtatca atcgtttcat
     5341 tgtggcaatt cggattagca gatatattgg aaaagatgac aaatgaagac gtattagttg
     5401 tgaccggttc actttatttc atttcagaag ttcgccattt attaatacaa ttaggaggag
     5461 atcatgaagt ataatgcagt gatttttgac atggatggtt tgctgttcga cacagagtta
     5521 gtgtattacg aagccacaca agtagtagca gacaagatgg gttttcctta tgataaagac
     5581 ctgtatttaa aattcttagg tgtttcagat gaagaagtat gggcgaatta tcatatgatt
     5641 ttctcggaat ttggcaaaac aagtgtacaa agattcatag atgattcata ccaggaaacg
     5701 cttgaacgtt tttcgcaagg agcggtacaa atgaagcccg gtgtgttgga actcctctct
     5761 tttttagaag aacatcagat tcccaaagtt gtcgcttcca gtaaccaacg agcagtcatt
     5821 gaacttttat tgaagaagaa caatctgttt gaacgatttg agacgattgt ttctgcagaa
     5881 aatgttcaac gagcaaaacc agacccagaa attttcttgt tagctaatca gaagctaggt
     5941 acagcgaaga aagaaacatt gattttagaa gattcacaaa atggtatttt agcagcttct
     6001 gcggctgaga tcccggtagt catggtacca gatctgcttc caccatccca agaattgatc
     6061 aaaaaaacag ctggggttgt ttcttcttta catgaaatcc ctgcattgtt aaaataaaaa
     6121 tccatctctt ttcgtatctt attttagata agatagaaag gagatgtttt tttgggaaaa
     6181 cgattgatca aagaagtacc tactagctct ttaccaaggg aacgtatgga aatttatggc
     6241 gcggaagctt tatcggatca ggagcttttg gcaatcttac tgcgtaccgg tcaacaccct
     6301 tatagtgtga tgtcgattgc tggaaatctg ttaaaaacgt ttggaggact ggcctcttta
     6361 cgacaggcta ctttgcatga gttagaagag attcgaggga taggacgcgt gaaagcaatc
     6421 gaaatcaaag ccttgatcga attaggtcga cgaattcaga cggatcatga aagagcggct
     6481 ccaccggttc gtaccagcta cgaattggcc cagcaattga ttctggaaat gaaagatcac
     6541 aaacaagaac atttggtttg tatttatttg gacacgaaga atcaagtgat cttaaaaaaa
     6601 acagtgttca tcggttcgct gaatcagtca gtcgctcatc ctagagaaat tttccattat
     6661 gctgtacgtt attgtgcagc aaggatcgtt ttagcgcata atcatccttc tggtaatgtt
     6721 ttaaccatag tataataaat aatagtagtt aaagtagttt aagaggagat attaaaggga
     6781 tattttactt taaataaaaa ttaacaaggt ttataatttg tagtagttaa agtatttaaa
     6841 gaggtgggaa catgtgaagg ttcaaaggat agaagtggag aataagccgt atccattgta
     6901 tttattacta gataaagaat accagctaat agaaccagta atgaaattta ttaaatactt
     6961 agataatact ggtaagtctc ctaataccat taaagcatac tgctatcatt taaagttgct
     7021 gtacgagttc atggaacaga gaggtgttat tcttaatgat attaactttg agttgttagc
     7081 agacttcgta ggttggttga gatatccttc agcatcaaat gtaattgatc ttcagtcaaa
     7141 aaaagccata agagaagaaa cgacagtgaa tacaatttta aatgtagtta tgagttttct
     7201 tgattattta agtagattag gagaatttaa atcaattgat gtatttaaac aagcaaaggg
     7261 aagaaatttc aaaggatttt tacatcatgt taataagggt agataccaaa agaatgtctt
     7321 aaagttaagg gttaaaaaga aacagataag aacattgaga tcagaggaag ttaagcaaat
     7381 tattgatgct tgtcatacga aaagagataa attaatttta atgcttatgt atgagggtgg
     7441 tttaagaatc ggtgaagtgt tatcgcttag gcttgaagat attgtcactt gggacaatca
     7501 aatccattta acacctagag atgttaatgt taatgaagct tatattaaat taaggaagga
     7561 aagaacaata catgtgagta aagaacttat gtcactttat acagattact tgatatatga
     7621 gtatagtgag gaattggagc atgattatgt ttttatttcc ttaaaagaag gctattttgg
     7681 gaaaccacta aagtaccaaa gtgttcttga tctagttaga agaatagtta aaaggactgg
     7741 aatagaattt acatcacata tgcttcgcca cactcacgca acgcagctaa ttagggaagg
     7801 atgggatgtt gcgttcgttc aaaagagatt aggtcacgca catgttcaga caacgttaaa
     7861 tacctatgtt catctttcag atcaggatat gaaaaatgag tttaataaat acctcgagag
     7921 aaaggagcat aagaaatgaa tgcttctagt aaaaggaaaa ttattagtca gagtgagatt
     7981 agcaaaaaaa tagctgtaat gaatgaagaa atgcaggggt tttgggctaa taatagttgg
     8041 gatataagaa aatgtccaca tccttctgcc atagaattaa gtaagaatcc tgctttaagg
     8101 aatcgttggg ttcgttttga acgtgttaaa aatctgtggt taagaacaga attgaaatat
     8161 ttttattttt accatttaaa caatggaata tggaatgcaa aaactgtctg gattagaaaa
     8221 ggaacagtaa ttaataaaat gttagatttc ttagatttaa agtatcctag cattacttca
     8281 attactgaag ttcctattga aaaagcaatg acggagtata gaacttattt gacaaaacgg
     8341 ggtgttagaa ttaccactac taattataag attactgcta atcaagaaaa aacacctgta
     8401 aaagctaatt cctactatgt tactaatcta aaacaattta tggagtttta tgagaacttt
     8461 tattttgatg gagaggagtg ggataaagac gtttgggata gacgtaactt acctttgcca
     8521 gatgataagg ttaacccaac acaatatgaa tatacaatta actttaaagg gtttcggaat
     8581 acatatttta aacaacttgt aaaaagatat tgtaagttga gattgaacat ggatagcttt
     8641 tcctatgtaa gtgatattgc ccaaagactt aaagagttct ttaattttct ggacatgaaa
     8701 tttaaacaag ttcagagagt acaccaatta acgagagtgg aaattgaagc atatttaagt
     8761 gaactaaaca tgatgggaat aaaacctagt acaataactg ggaggatctc tatattggaa
     8821 ggactattta gtacccttct taggctagaa tgggatgatg ttccttccaa aatattaatt
     8881 tattctgagg actatccgaa aataccaaga gcaaaaccac gctttataga tgaattcgtc
     8941 ctagagcaat tgaacagtca tcttgataaa ttacccgaat atatagctac gatgactatg
     9001 attgttcaag aatgtggaat gaggataagt gaattgtgca ccttgaaaaa aggctgtcta
     9061 ttagaggaca aagatggaga tttcttttta aagtattatc aatggaaaat gaaaaaggag
     9121 catatagttc caatatctaa agaggtagct ttacttatta aagttcggga agataaagtt
     9181 tcagaggaat ttccagatag tgaatacctc tttccaagaa aagatggatc gccattaaaa
     9241 caagaaacat ttagaggtga gttaaataaa ttagcttatg agcaaaatat agtggataaa
     9301 tcaggtgaga tttatagatt ccatgcccat gcctttcgcc atacagtagg aacaagaatg
     9361 attaacaacg ggatgcccca gcatattgtg cagaaatttt tggggcatga aagcccagaa
     9421 atgacaagca gatacgctca tatctttgat gaaactctaa aaaatgaatt tactaaattt
     9481 caggaaaaac tggttaccaa taatggagat gtgcttgatc tagatgaaga taatgaagtc
     9541 gatgatgtag agcttcaatg gttcaagaaa aatataaatg cacaagtgct tccaaatggt
     9601 tattgtagat tgccagtagt agcaggtggt tgtccacatg cgaatgcatg cttagattgc
     9661 actcacttct gtaccagtaa gcaattctta ccacagcacg aagaacagtt agagcgtaca
     9721 gaagagttat tagccatagc taaggataaa caatggcaaa gacaagtaga gactaatagc
     9781 cgtgttaaag agcgtttaga acaaatcatt ggaagtttga cggggtaatt atcaatggat
     9841 aaacaagtta gaaatacaac agaaattgta cgtttggcga agcagaaatc aaaaaagaca
     9901 agggaaaaag tagacaaagc gatttctaaa ttttcgattg aaggtaaagt tattaatttt
     9961 aattcaatag caaaggaagc taatgtttct aaatcatggc tttataagga acacgatatt
    10021 aggcaaagaa tcgaatccct tcgtgagcgt caaataacag caaatgtagt ctcaaaaccc
    10081 aagaaaagtt ctcgttcgga ggaaatcctt attaaaacct taaaaagaag agtaatggaa
    10141 ttagaaaaag aaaataaaaa attacagaac caaattcaaa aattatatgg agatctgtat
    10201 aataaagaat aattattaat ctgtagacaa attgtgaaag gatgtactta aacgctaacg
    10261 gtcagcttta ttgaacagtc atttaggtat atgtccaatc tagggtaagt aaattgagta
    10321 tcaatataaa ctttatatga acataatcag ttacgaggtg aaatcatgag caatttgatt
    10381 aacggaaaaa taccaaatca agcgattcaa acattaaaaa tcgtaaaaga tttatttgga
    10441 agttcaatag ttggagtata tctatttggt tcagcagtaa atggtggttt acgcattaac
    10501 agcgatgtag atgttctagt cgtcgtgaat catagtttac ctcaattaac tcgaaaaaaa
    10561 ctaacagaaa gactaatgac tatatcagga aagattggaa atacggattc tgttagacca
    10621 cttgaagtta cggttataaa taggagtgaa gttgtccctt ggcaatatcc tccaaaaaga
    10681 gaatttatat acggtgagtg gctcaggggt gaatttgaga atggacaaat tcaggaacca
    10741 agctatgatc ctgatttggc tattgtttta gcacaagcaa gaaagaatag tatttctcta
    10801 tttggtcctg attcttcaag tatacttgtc tccgtacctt tgacagatat tcgaagagca
    10861 attaaggatt ctttgccaga actaattgag gggataaaag gtgatgagcg taatgtaatt
    10921 ttaaccctag ctcgaatgtg gcaaacagtg actactggtg aaattacctc gaaagatgtc
    10981 gctgcagaat gggctatacc tcttttacct aaagagcatg taactttact ggatatagct
    11041 agaaaaggct atcggggaga gtgtgatgat aagtgggaag gactatattc aaaggtgaaa
    11101 gcactcgtta agtatatgaa aaattctata gaaacttctc tcaattaggc taattttatt
    11161 gcaataacag gtgcttactt ttaaaactac tgatttattg ataaatattg aacaattttt
    11221 gggaagaata aagcgtcctc ttgtgaaatt agagaacgct ttattacttt aatttagtga
    11281 aacaatttgt aactattgaa aatagaaaga aattgttcct tcgatagttt attaatatta
    11341 gtgacatttg catgcttcaa agcctgtcgg aattggtttt tagtgaaaag aacacgatat
    11401 tcacggttta cccacttata aacaaaagat cgatactttt tgtagtcctt ctttgaaatc
    11461 aatggttgat gtcgttcaag aacaatcaat acagagtcta cacttggctt aggatgaaaa
    11521 tatagtggtg gtactttttt gagcattttt atatccatct ccaccattaa tagtaaaccc
    11581 aaagctcgtt gcagattttg caatcttttc gcaaatccct tctcaacgat aagatagcta
    11641 tatttagcct gactttcaaa ggtaattctt ttgacaatat ccgtactgat gttataagga
    11701 atattaccat atatcttata gtttatatgt tttgggaagg aaaattttag aatatccgtt
    11761 tgaatcactt ttatattctc agaggggttt accgcttctt tagtcacttg acataagcct
    11821 ccatcaattt ctatagcagt aactgatcga ctcattttga ctagctcttt ggtaaaatgt
    11881 ccttttcctg atccgatttc tattacgttg tcttgtttac tgatattcgt gtgattcaat
    11941 atttctttta catgcttttt agaagtaata aaattttgcg tgtctttagg gtttttctgg
    12001 ttcattataa ccttctcctt actggttata atgaactgac attaagcgtt cattataacc
    12061 aattaatttt gatttggttg ataacgaact ttattaataa caaatataga aaaagtaccc
    12121 atacttttat ctcctttcca tttttttcat tatgaatgag ataaagtaat ttctactact
    12181 gcgatactgg tgcacataat tgaaacctcc ttatgtaact gaatatgatt gtatcatctt
    12241 ttcattttag aataaacctt tatatgtaat tttgcacttg acacttcaga tggacgtttt
    12301 cttcaaaata atatgttttg aaacttgata agagaagcca ctgtctttac taaaaagtaa
    12361 aggctgcgat aaaggacagt gactttaata aagttgcact gttgtattgt ctttggataa
    12421 aattgtctaa agggaagttt tatccccaac gaaaaaacat ctaaagtggt aagtaatgaa
    12481 agaatgtcga taaagtagga atgggggatc tttttgaata caatagaatc acagtttgat
    12541 aaggttgcag aagattacga tttcgtgaat gagcttttga atgattattc attctttgtg
    12601 tctaatatgt ctccaaagaa aggcagagca ttagatatcg gatgtggctc gggtttgtta
    12661 gtggagaaat tagcaagtta ttatgatgaa gtggtaggga ttgatatttc taatcaaatg
    12721 ctcgatcttg ccaaatctaa acgtcaacta acaaatacgg tctatctgaa tatgaatgca
    12781 gaacaactta attttaatga gaagtttgat tttattgtaa gccgaacaac ctttcatcat
    12841 ttggatgata tagccagcgt aatacagcaa atgaaggaac tgttgaatga ggaaggaaga
    12901 atagttattc ttgataatgt atctgaagtc gagacaccgc ctacctatgt ttataaatta
    12961 ggggcaatcc aagagttttt accgcattgt ttcaaatttg gaataaaaaa tgcgattaga
    13021 atttataatc acaacacatc taaatcatgg cttgagcatt tagcttcaga taagtaccta
    13081 tctgaacaaa attattacga tttatatgaa aagttgttgc ctggatgcca attccataaa
    13141 atgggttggg caatgggggt tgtctggaca aaataactta aagctgtggc tttgaaaagt
    13201 tgaatttgtg attgttttca gagctatcgg gtgctttagt ataaataaca atgaataaaa
    13261 aatcagcctg
//
LOCUS       DARBFD010000077        12943 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000077 DARBFD010000000
VERSION     DARBFD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.077"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SCK08_002102"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SCK08_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEG1201957.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /locus_tag="SCK08_002103"
     CDS             complement(1195..3036)
                     /locus_tag="SCK08_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEG1201958.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="SCK08_002104"
     CDS             complement(3218..5533)
                     /locus_tag="SCK08_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1201959.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="SCK08_002105"
     CDS             complement(5718..6578)
                     /locus_tag="SCK08_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEG1201960.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7158)
                     /locus_tag="SCK08_002106"
     CDS             complement(6637..7158)
                     /locus_tag="SCK08_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEG1201961.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7436..8197
                     /locus_tag="SCK08_002107"
     CDS             7436..8197
                     /locus_tag="SCK08_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1201962.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="SCK08_002108"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="SCK08_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1201963.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SCK08_002109"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SCK08_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1201964.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="SCK08_002110"
     CDS             12557..12913
                     /locus_tag="SCK08_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1201965.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2708 c         2300 g         4222 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaa
//
LOCUS       DARBFD010000078        12773 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000078 DARBFD010000000
VERSION     DARBFD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.078"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..290)
                     /locus_tag="SCK08_002111"
     CDS             complement(<1..290)
                     /locus_tag="SCK08_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1201966.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="SCK08_002112"
     CDS             complement(283..624)
                     /locus_tag="SCK08_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201967.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="SCK08_002113"
     CDS             complement(798..1523)
                     /locus_tag="SCK08_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG1201968.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="SCK08_002114"
     CDS             1576..2052
                     /locus_tag="SCK08_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201969.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="SCK08_002115"
     CDS             complement(2058..2240)
                     /locus_tag="SCK08_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201970.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="SCK08_002116"
     CDS             complement(2240..2539)
                     /locus_tag="SCK08_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201971.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="SCK08_002117"
     CDS             complement(2644..2847)
                     /locus_tag="SCK08_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1201972.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..3608)
                     /locus_tag="SCK08_002118"
     CDS             complement(2862..3608)
                     /locus_tag="SCK08_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEG1201973.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3686..3994
                     /locus_tag="SCK08_002119"
     CDS             3686..3994
                     /locus_tag="SCK08_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201974.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3965..4222)
                     /locus_tag="SCK08_002120"
     CDS             complement(3965..4222)
                     /locus_tag="SCK08_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEG1201975.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4520..4660)
                     /locus_tag="SCK08_002121"
     CDS             complement(4520..4660)
                     /locus_tag="SCK08_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1201976.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            4960..5280
                     /locus_tag="SCK08_002122"
     CDS             4960..5280
                     /locus_tag="SCK08_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1201977.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            5298..5729
                     /locus_tag="SCK08_002123"
     CDS             5298..5729
                     /locus_tag="SCK08_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1201978.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            5856..6440
                     /locus_tag="SCK08_002124"
     CDS             5856..6440
                     /locus_tag="SCK08_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG1201979.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            6542..7690
                     /locus_tag="SCK08_002125"
     CDS             6542..7690
                     /locus_tag="SCK08_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1201980.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(7779..9404)
                     /gene="groL"
                     /locus_tag="SCK08_002126"
     CDS             complement(7779..9404)
                     /gene="groL"
                     /locus_tag="SCK08_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEG1201981.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(9455..9739)
                     /gene="groES"
                     /locus_tag="SCK08_002127"
     CDS             complement(9455..9739)
                     /gene="groES"
                     /locus_tag="SCK08_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEG1201982.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            9964..10626
                     /locus_tag="SCK08_002128"
     CDS             9964..10626
                     /locus_tag="SCK08_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1201983.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(10702..11994)
                     /locus_tag="SCK08_002129"
     CDS             complement(10702..11994)
                     /locus_tag="SCK08_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1201984.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            12164..>12773
                     /locus_tag="SCK08_002130"
     CDS             12164..>12773
                     /locus_tag="SCK08_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEG1201985.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQH"
BASE COUNT         3888 a         2466 c         2164 g         4255 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcactc gttacccaac gtttaaattt tttggcagag
     3361 ggaagttttg attttaagat taaactgtaa aggcctgact cgttaatcaa aacaactgct
     3421 gttccattga cggtgaacga atcgttctgc gtcttatcct ccaagtccac atgatctcga
     3481 atagcttttt gcggattcga atatcctaga acgtctgcta catcttttcc tacaaaatat
     3541 ggttcatcat ttactaaaat agttcgaact tcgttttgct cgaaattaaa aatttgtggt
     3601 gtattcatat ttctcattcc tttctttggt ataatttcct tatcagtgtg acaggctgaa
     3661 ataattgtta aggaggtgaa aatttatgaa accagaattt gtaattacta tgcttgatgg
     3721 agaaaaaatc aaaatatttg ccgatacttt ggtcgttgga tacgatgatg cgtcaaatat
     3781 cgaagaaaaa gatggaatgt tctattcttc tcaaatttac gtgaatacct tgcaaggtga
     3841 tattggggta tctactcttg caacatcaag accgcttgta ggtcttatcg gctttttgtc
     3901 aaaagcaaca ttcttttctc tcggcgatga tatagattcg gatgaaactg tcatctataa
     3961 aacatcagct gttaaaagta tcggtttcaa atagtaattc ttgctcgttg atagcggaag
     4021 ccaacttttc ttccaacaca tcggcgagct ttctttttgt tggattcatt tcaaagttga
     4081 tagtcttgag aagcattttt gcatccttaa ctttttgtcc ttgcaattcc ttgatgattt
     4141 tatcggcttg gctatcaatt ccatgtgtta gatcatctat cattttttgt accttgattg
     4201 tttggctcat ttccttgttc attgtttagc ctcctttaat aaatgatttt ttgtattttt
     4261 taatttccat aaattcctca aattgctctt tggtattatt accacgacca tacatattgt
     4321 gaaattcttt atggcaactg acacaaagtg taacgccgtt tgaaatcgct gcggatttag
     4381 ttgttgcatg gctaaatcac gaatcacaag tatctactaa aggcgaatca atttcgccaa
     4441 aagaaattgt tcaagcttat ttagatattc attatgctgt tatctatggt cagcttccag
     4501 aagaccgtaa aaacgatgac taaacgtgag ctaaaatctc tgctatggct gcaaccatgg
     4561 cagagtcttc tttatctacc gcgtgttcca tcgccacttt tgcttgtttc agtatgtcta
     4621 atttcaaatc ttcgattttt gccgagactg ttttttccat attcttctct cctttctttt
     4681 atattcgtaa acaaatttaa caacttttta aaaaactgcg ttgactttat tagattaata
     4741 atctatacta aatgcatagt taaataagac atataaactt tgatttaaaa agctttcttg
     4801 gcggttggca tttattaatc aatagtattt tttgttgtct ttttagttgt taaacttgtt
     4861 tacaaaacat agtatagatt aataatgtat ataagtcaac tatttttaca ttatttatct
     4921 aaactttttt tgtaagcatt cagaaaggtt gatttatcaa tgaatactta cgaaataata
     4981 aaggagttag caaaagaaaa aggattatcc attagacagc tcgaaatgaa tttcgggtat
     5041 tcgaatggat atttaggaag ttggaaaaga caaactccta attctattga gttagctcgg
     5101 ttagctgatt actttggagt ctctgtagac taccttctag gtagagaaga aagagaaacc
     5161 cctaaacatg tggatttatc agaaaacgat actgtttttt cttttgatgg aaaagaaata
     5221 tctaaggaga caatgcgtaa agcgattgca attgctaaag ctttagagga aaatgaatag
     5281 ttggagtgat gggttgtatg tatttgaaat tgaaagaaat gttgagcgaa tacaatttga
     5341 aagtagttta catggaaatg aaagagcctg gcttctatta tccgaagcct agaatcattt
     5401 ttttgaatga aaatctatat ggcgaaactg cagaagcctt tcatttatct catgaacttg
     5461 cgcatttcac cgcttctcat tttgaatatt cagttttgta cgatacttct acaacttttc
     5521 actcaaaatt tgaaacagaa gcagataaaa tagctattct aattttatta aatatctata
     5581 ttgagaacga actgactgat gaatctcagt ttaatctcga aaaatttatg gagtattatt
     5641 ctattcagaa taagctcaga tacacttgtt atgctgtctg ccaatgctat tttaagaaaa
     5701 aatattctta cgcaaggcaa tatgtatgaa tacatcaaga attattaaaa aaaccgattt
     5761 cagcaaaaca ttaaaaaagc ccgtgctggc acacggactc atacctcatt tctgagatcg
     5821 caaatatatt ataacaagaa gtgaggaata tttaaatggc aaaaaaagtt atgggtcaag
     5881 acggaaaaat gtataaagtt agtaaacctt tttacaaaaa agtatggttt tgggtattag
     5941 cagtaatttt aattataatt attggttctg ctttaaatgg tggatcagat agtaataaag
     6001 caagtgataa tggtggcgaa aaagtaacta aatcttcaac ctctgcttca tcttctaaag
     6061 aagaaaaaag cgataccttc tataagattg gtgacactgt aaaagttggt gatgctgaat
     6121 atacactaaa tagtgtagaa ctaactgatg aaagaaatca atttgaagaa aaccaacctg
     6181 cacaagtagt aaaaattact tataccgtaa aaaatgatgg cgattcagat atccctgtag
     6241 gtacagatgt ggaagtatat ggaccagatg acaaaaaatc agagacatat gccaacgaaa
     6301 atacaatggg atctgttgct ccaggaaaac aaatggatgt aactgctcat ttcacgttaa
     6361 accaaaaggg agaaatagaa atccacttct ctcctttagt atcatttgaa aaagcagcta
     6421 tttttaaagc aactgtataa taaataaaaa acacgcccca ccgtccagag taagagcgtg
     6481 cttttagaaa acaaacctat ataggtctat ttgttatgcc tattatagca aatgatagga
     6541 gatgttaaaa gtgtgggttg aacagaccaa agatggccaa ttcaagttta ttgaaagata
     6601 tgtagatcct tatacagaaa aaacacgaaa aaaatctaca acacttacaa gtaattcacc
     6661 acaagcgtgg aaaaaagctc agaaaattct agataaaaaa attaaagaag cactcgaaga
     6721 ttacaataaa tcagatatca cttttggtga gctgtataaa gaatggtatg aatattataa
     6781 gcagcatgtt aaacgtacta gctatttgaa ggttccaatg atgatgaaac atgtttctaa
     6841 gcacataagc gatgatacga tcgttagaaa cattgatgag acactcatta ataagataat
     6901 tgaagatatg tatacgtttg gtgacctctc actgaactac acaaaacaaa caaaaacaac
     6961 tctatctgtt atgctaaact atgcaatcga tagaaaatac attcaaagaa accctgcgct
     7021 agcagttaaa atccatccta aaaaagtgga agaagaaaaa agaaggcttt ctatgaataa
     7081 aaaatatctg gagaaagaag aaattgatca aatactgaaa cagctatact ccaatcctcg
     7141 cagaaaacta cacggcataa tagctgagtt tctatattta actggtttga gatatgggga
     7201 attgctagct ttgcaaatga aggactatga ggatgggaaa atttccatta atgggacctt
     7261 agattacaca tctgtgaaaa tggataatgc tataaaaaca actccaaaaa atacttattc
     7321 gcaacgtgaa gtgcaattac ccaatcgtgc aaaagaattg attgaaagtg tgatagctga
     7381 caatattctt gcaggtagac ccacagatcc cgatcaatat atatttatat ctacaagtgg
     7441 cactccgctt gcgctgcact catttaacgc tatactccat aaagtagagg aagagttgga
     7501 attagaaaaa agtctatcct cacatatatt cagacatagc cacgtttcac tattatctga
     7561 attaggcgta cctcttaaag ccattatgga gcgtgtaggg cattctgatg caaacacaac
     7621 tctgtctatt tataaccatg taacaaaaag agccaaacaa caagtaattg ataaactaaa
     7681 tagcctttga tatttttgcc ccttctttgc cccttttgct taaaataaaa caaaaaaaga
     7741 agaaagaacc ctgttaaaat agggttcttt cttaattact acatcatacc gcccatcatt
     7801 gatggatcca ttgcaggagc tgctggcgct gctggttctg gtttatctgc aacgactgct
     7861 tctgtcgtta ataacaaggc agaaacggaa gcggcatttt gcaaggcaga acgtgttact
     7921 ttagttgggt ctacgatacc tgcttcaacc atgtttaccc attctccagt agcagcattg
     7981 aaaccagtgc ctagttccac gttcttcaat ttatcaacaa tcactgatcc ttcataacca
     8041 gcgttttcag cgatttgacg aattggttct tctaaagcac gaacaacgat cttgatacct
     8101 gttgccacgt ctccttctgc ttctacagca gaaactttgc tgataacgtt cactagagct
     8161 gtaccaccac ctgagaccat tccttcttct actgcggcac gtgtagcatt caatgcatct
     8221 tcaatgcgta attttaattc tttcaattct gtttcagtag ctgcaccaac tttaacaaca
     8281 gctacaccac ctgctaattt agctaagcgt tcttgtaatt tttcgcggtc aaaatcagaa
     8341 gttgtttcag caatttggtt tttgatcaat tgcacacgag cttcgattgc ttctttttct
     8401 ccagaacctt ctacgatcgt tgtattgtct ttatctacaa caactttaga agcatttcct
     8461 aagttttcga ttgttgcatc tttcaattct aaacctagat cgtcagtaat tactgtacca
     8521 cctgtcaaaa tagcgatatc ttcaagcatt gctttacgac gatcgccgaa accaggagct
     8581 tttacagcta ctacgttgaa tgttccacgg attttgttca atacaagagt tggcaatgct
     8641 tcaccatcta catcgtcagc aataatcaac aatggacgtg attgttgcaa gatttgttct
     8701 aacaatggca gaatatcttg gatgttagag atttttttgt ctgtgatcaa gatatatggg
     8761 ttttctagaa cagcttccat tttgtcgtta tctgttacca tatattgtga taagtaacca
     8821 cgatcaaact gcataccttc tacaacatct agttctgttt cgatcccttt tgattcttcg
     8881 attgtgatga caccgtcgtt tccaactttt tccattgcat cagcaatcaa atgtccaact
     8941 ttatcagaac ccgaagaaac agctgctact tgtgcaatgg cttcttttga atcaacaaca
     9001 gttgagatat tatgcaattc ttcgactgct gcttttgttg ccaattcgat cccgcgacga
     9061 atgcctagag gattagcacc tgcagttacg ttttttaaac cttcgcgaac gattgcttgt
     9121 gtcagaacag ttgctgtcgt tgtaccgtct cctgcaatat catttgtttt tgatgctact
     9181 tctgatacaa gttttgcacc catgttttca aaatgatctt ctaattcgat ttcttttgcg
     9241 atcgtcacac catcatttgt gatcaatgga gagccgtaag atttttctag tacgacattt
     9301 cggcctttag gtcctaatgt tactttcact gtatctgcta atttatctac cccgcgtaac
     9361 atagctgcgc gtgcgtcttc tgcaaatttc aattcttttg ccatgattga ttcacctcat
     9421 tgattatttt tgattatttt tttatcttcc aaatttactc aacaatcgcc atgatatctt
     9481 ttcctgcgac gatcagatat tcttttcctt cgtattttac ttctgtaccg gcgtattttt
     9541 cgaacataac ttggtcgcct gcttttacag cggcaggaac tttttcaccg ttttctaaca
     9601 aacggccttc accgactgca acaactgtcc cagtttgggg tttctcttta gcagcagaag
     9661 ctaaaacgat tcctccgaca gttttttctt cttcttttgc gacttcgata atgacgcgat
     9721 cacctaatgg ttttaacacg ataaatccct ccgataactc tttttttaat ctgttagcac
     9781 tcttaacctt cgagtgctaa cctacatttt taataatact cattctccct cttttttgca
     9841 agcttttaaa tcaaagaaaa gaaattttat tctgacagct gcttattgaa attatgatat
     9901 aattgaaatc aatcgatata agcttgcctt gcatgcttgt tatcccttaa aaggaggaat
     9961 tacatgtctt taaaaaaata tagcttcctc agcatttttt gctatggtct aatttttatc
    10021 tcacccttga tcctagcttc gataggactt gtaaaatcta cgtctgaatt gatcacagca
    10081 actgcagtca tgtatatact aggcgccgtt gtcctagcta ttttttattt taagcaacgt
    10141 gaacccttag cgatcgaatc agcggtaaaa aaagcttctg ctccaaagat tgttatttat
    10201 ggattggttg gaatctttgt cgcattgatt ctgcaaagca tcgctgtcat gttagaatct
    10261 gtattgtttg gcaaagcaac cccttccgag aatacacaaa atatcatcca gatgatcatg
    10321 gaagcacccg cttttatttt ggcgacaact gttgctggac ccatcatgga agagttcgta
    10381 ttccgccgca gtatactggg gatcatcagt cgatattcaa atttttgggt cggagcagtg
    10441 atcagctctt tgctgtttgc gtttgctcat aatgatggtc atctgctaat ctactttttc
    10501 ctaggtttct tcttcagcct agagtacaaa gcaactggtc ggatctggac atcgatgatc
    10561 acacacgtag gaatgaacac attagttgtt cttgttcaat tagctgtcca aaaagggcta
    10621 gtataaacat gactataaat actcctgtca tcctagattg gtgattttcc caatctaaag
    10681 cgacaggagt atttttgttt tttagtcgtt catcttttct gctgctccat cattttttgt
    10741 ctgtctttcc tttttgcagt ttgcagaatc tatttttttc aaaaatggga aataaatgaa
    10801 aaaactaatt gacaacacga taccttgtaa aatcgctgca ggcactccgc ctactaagaa
    10861 acccgagagg atcggcggtg ttgtccaagg gacttggact gctgtaaata aaggaacgaa
    10921 tccaaaatat aaagcggaat aggtaatcaa tcctgaaacg accggtgcga aaataaaagg
    10981 aacagccatc atcgggttca tcacgatcgg tgtggaaaaa ataattgatt cattgatgtt
    11041 gaaaaaggct ggtaaaagag caagatttcc taattctctg tatttcttcg atttcgcaaa
    11101 aaccgtcata aaaaatacag cccctaacgt caatccagca cccgtaaccg tcatgaactg
    11161 atcgagaaat tgctgagtaa cgatatgtcc gccatttgcc accgtcaact cttgtcctga
    11221 ggctaagatt tctgcatttg ctaatgaatt cgcttgcaag acgggactaa ccaacccatt
    11281 gaccatcgca gatccatgaa gaccaaataa ccataaaaac ggaacaaaaa aacctaataa
    11341 aagcacacca aacaacgtgt ctatgaacga ttgaatggga agctggacat atgtccatac
    11401 tccttgaaca acagtcattc tgaacaaatg ttgaaaaacc gctgaaccga taagcgtaaa
    11461 agtgactata acaatgattg gaatcaatga agtaaaagac tgccaaacat tttcaggtat
    11521 cttctctctt tgcttccacc ctttcgcaag tcttactaaa cgtgcatagc cccatccgat
    11581 gatgagccca gttgtaactg ctcctagtat cccttcagca cctgtccaga cagtatctat
    11641 ctgagtattt agtggctgaa taagcagtag ataagcagac aaggctgtta tgccgactgg
    11701 taagcgatcg atgtcttctt cttttgcata agcgtatgca atggcaaaaa cgatccaaaa
    11761 ggctatcaca tcaaatgtag cctggtatac ttgtgtaaaa tacatagaaa atcctgtttt
    11821 gtctatccac tgagcgatgt aatccacagg aaaatgagca aataagaaaa acagcgagcc
    11881 aacaataatt attgggatcg tgtaagtcat tccattacgc aaagcagtta agggtctaag
    11941 attcacaaca gatgttatca ttggtaaaaa ggcacgatct aaccactctt tcatttctta
    12001 tctccttcaa cttctatttt tatcaatcag ttttcttaat ttaactatac ccaatgatca
    12061 cgaaccatga aaggaatgcg cactgagctt ttcaaaaatc ggattctttg acgaatattc
    12121 agcaactgct tacaatggac aggaataaaa aaggaggttt tttatgaaaa ttgcactcat
    12181 tggagctact ggccatgcag gttcatttat tttagacgaa gcattgtcca gagatttaga
    12241 cgtgacggca atcgtgcgtc atccagaaaa attgccatca gatgttccgt ttatacaaaa
    12301 ggatttattt gagttgacaa ctactgatct tgcatctttt gatgttgtcg ttgatgcttt
    12361 caacgctccc aaaggaaaag aagaaatgca tcaaacttct ttgtcccatt tgattgagat
    12421 gcttgaagga accgatacac gcttaattgt cgttgggggt gcctcttcgt tattcttaga
    12481 tacagaaaag acgactcgta tgattgacca agtacctaaa aatgcaccct tctatccaac
    12541 agctttcaat atgtacgaag gattgttaaa attgaaacaa tctaaaaatc tacattggac
    12601 ttatatcagt ccagcttctt tatttgatcc acatggcaaa cgtacaggta cttatttgtt
    12661 aagtgatgat tatttacaaa gaaacgctgc tagcgacagt acgatcagca tggcagatta
    12721 cgcgattgca ttagtagatg agatactgga tgagaaacat gaaaagcagc aca
//
LOCUS       DARBFD010000079        12734 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000079 DARBFD010000000
VERSION     DARBFD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.079"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCK08_002131"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCK08_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1201986.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="SCK08_002132"
     CDS             complement(322..891)
                     /locus_tag="SCK08_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEG1201987.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="SCK08_002133"
     CDS             1077..1979
                     /locus_tag="SCK08_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1201988.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCK08_002134"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCK08_002134"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEG1201989.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCK08_002135"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCK08_002135"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEG1201990.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCK08_002136"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCK08_002136"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEG1201991.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="SCK08_002137"
     CDS             8547..8732
                     /locus_tag="SCK08_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEG1201992.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="SCK08_002138"
     CDS             complement(8794..9990)
                     /locus_tag="SCK08_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1201993.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="SCK08_002139"
     CDS             complement(10160..11596)
                     /locus_tag="SCK08_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1201994.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="SCK08_002140"
     CDS             11889..12698
                     /locus_tag="SCK08_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1201995.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DARBFD010000080        12611 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000080 DARBFD010000000
VERSION     DARBFD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.080"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SCK08_002141"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SCK08_002141"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEG1201996.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SCK08_002142"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SCK08_002142"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEG1201997.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="SCK08_002143"
     CDS             complement(2303..2965)
                     /locus_tag="SCK08_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1201998.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="SCK08_002144"
     CDS             3149..4795
                     /locus_tag="SCK08_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG1201999.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="SCK08_002145"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="SCK08_002145"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1202000.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="SCK08_002146"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="SCK08_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="SCK08_002147"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="SCK08_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="SCK08_002148"
     CDS             complement(8944..10023)
                     /locus_tag="SCK08_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1202001.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="SCK08_002149"
     CDS             complement(10126..10566)
                     /locus_tag="SCK08_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1202002.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="SCK08_002150"
     CDS             complement(10623..11033)
                     /locus_tag="SCK08_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1202003.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="SCK08_002151"
     CDS             complement(11213..11647)
                     /locus_tag="SCK08_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1202004.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="SCK08_002152"
     CDS             complement(11747..12595)
                     /locus_tag="SCK08_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1202005.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         4217 a         2347 c         2242 g         3805 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta a
//
LOCUS       DARBFD010000081        12209 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000081 DARBFD010000000
VERSION     DARBFD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.081"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(82..639)
                     /gene="frr"
                     /locus_tag="SCK08_002153"
     CDS             complement(82..639)
                     /gene="frr"
                     /locus_tag="SCK08_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEG1202006.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(642..1364)
                     /locus_tag="SCK08_002154"
     CDS             complement(642..1364)
                     /locus_tag="SCK08_002154"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEG1202007.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1500..2381)
                     /locus_tag="SCK08_002155"
     CDS             complement(1500..2381)
                     /locus_tag="SCK08_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEG1202008.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="SCK08_002156"
     CDS             complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="SCK08_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEG1202009.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3621..4100
                     /locus_tag="SCK08_002157"
     CDS             3621..4100
                     /locus_tag="SCK08_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEG1202010.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4317..5408
                     /gene="phnW"
                     /locus_tag="SCK08_002158"
     CDS             4317..5408
                     /gene="phnW"
                     /locus_tag="SCK08_002158"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEG1202011.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5401..5529
                     /locus_tag="SCK08_002159"
     CDS             5401..5529
                     /locus_tag="SCK08_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1202012.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5533..6078
                     /locus_tag="SCK08_002160"
     CDS             5533..6078
                     /locus_tag="SCK08_002160"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1202013.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6532..8223)
                     /locus_tag="SCK08_002161"
     CDS             complement(6532..8223)
                     /locus_tag="SCK08_002161"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEG1202014.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="SCK08_002162"
     CDS             complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="SCK08_002162"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEG1202015.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9705..10724)
                     /gene="argF"
                     /locus_tag="SCK08_002163"
     CDS             complement(9705..10724)
                     /gene="argF"
                     /locus_tag="SCK08_002163"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEG1202016.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="SCK08_002164"
     CDS             complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="SCK08_002164"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEG1202017.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3343 a         2528 c         2203 g         4135 t
ORIGIN      
        1 tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc
       61 ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat
      121 cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg
      181 caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc
      241 acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttaa catctttagc
      301 taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc
      361 attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat
      421 gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat
      481 ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc
      541 gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt
      601 cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt
      661 gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag
      721 acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt
      781 aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct
      841 actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct
      901 gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg
      961 gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga
     1021 atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc
     1081 acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg
     1141 taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt
     1201 ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg
     1261 atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc taaagcttct
     1321 ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata
     1381 ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt
     1441 gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat
     1501 tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct
     1561 tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt
     1621 tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg
     1681 aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc
     1741 aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat
     1801 tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt
     1861 tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat
     1921 ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt
     1981 tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg
     2041 attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct
     2101 tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca
     2161 acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg
     2221 tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc
     2281 atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt
     2341 tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg
     2401 tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa
     2461 acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga
     2521 tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt
     2581 caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac
     2641 gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat
     2701 caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt
     2761 tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta
     2821 agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa
     2881 cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt
     2941 ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc
     3001 ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata
     3061 atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt
     3121 taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct
     3181 tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt
     3241 gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct
     3301 tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg
     3361 gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag
     3421 agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa
     3481 gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg
     3541 tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc
     3601 tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca
     3661 ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg
     3721 ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac
     3781 ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta
     3841 ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac
     3901 gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg
     3961 ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca
     4021 tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa
     4081 ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta
     4141 ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata catcattttt
     4201 tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta
     4261 acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt
     4321 ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga
     4381 tcgatcattg tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat
     4441 tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagca
     4501 gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga
     4561 tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga
     4621 actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc
     4681 tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag actacttccg
     4741 gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat cttttgttta
     4801 tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa ctagggattg
     4861 attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac
     4921 ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc
     4981 tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta
     5041 cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg
     5101 aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa atgacagaga
     5161 tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc
     5221 ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa aatggatttg
     5281 tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga
     5341 tctatccgga agatattttg gaactgacag caattatcaa agactataag gagaacgtta
     5401 atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct
     5461 cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa
     5521 atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct
     5581 tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag
     5641 gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg
     5701 aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc
     5761 acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg
     5821 tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg accgtttcct
     5881 tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa
     5941 gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt
     6001 gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg
     6061 gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc ggcgctgata
     6121 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac atgaatccag
     6181 aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa
     6241 cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6301 aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac
     6361 agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc
     6421 attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct
     6481 tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt
     6541 atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata
     6601 aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata
     6661 gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta
     6721 tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca
     6781 gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc
     6841 caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa
     6901 tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg
     6961 gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg
     7021 gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa
     7081 taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa
     7141 tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa
     7201 ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta
     7261 agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc
     7321 tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc
     7381 tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc
     7441 attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt
     7501 attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag cctctttatc
     7561 gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc
     7621 agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt ctgctttaac
     7681 agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca
     7741 gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat
     7801 cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg
     7861 agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc cataatgttc
     7921 tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa
     7981 gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc
     8041 taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg
     8101 aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct cgacttgttc
     8161 cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt
     8221 catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc tctagcgacg
     8281 gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct
     8341 aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc
     8401 caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca
     8461 agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt
     8521 atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaacaatcca gatggcctaa
     8581 aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa agacttaaca
     8641 aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc gatattttct
     8701 aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat agctgcttct
     8761 actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg tttcatttct
     8821 gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac atacacattg
     8881 tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa tttttctgaa
     8941 gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt ttctacaaca
     9001 ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag cgtttcgatg
     9061 acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct ccatccacgt
     9121 ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt ggcttcttct
     9181 tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc attttcatcc
     9241 acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc cttcagtact
     9301 tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc aacacatgta
     9361 tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat caataggtta
     9421 ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc gataaatttc
     9481 actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact tgcatccgta
     9541 gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat ttcattcgtc
     9601 ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt tgtgattttc
     9661 cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac gcgagggata
     9721 aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg attttctgct
     9781 tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc catttcagtg
     9841 atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc
     9901 aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat ttccatattg
     9961 atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc acccattgat
    10021 acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac atcgtcggta
    10081 atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac gatttcttct
    10141 tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg
    10201 actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac agtgatacct
    10261 tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg tgttggatgc
    10321 cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag ttcctctacc
    10381 attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag tactttagct
    10441 gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag atattcagga
    10501 tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt agatgttttt
    10561 tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc acgttttttc
    10621 aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt tgtaaagtct
    10681 ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa cattctcctt
    10741 tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca gtaagactgc
    10801 cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat gcagcgtgga
    10861 ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat gccatagcta
    10921 cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc tggagcgatt
    10981 gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc atctccatta
    11041 ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag
    11101 ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa gacatccatc
    11161 ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc taccattgta
    11221 aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc caacactttt
    11281 tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc tttcgcatta
    11341 gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc gtctccgcct
    11401 tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt tgcaaatctt
    11461 ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc
    11521 atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag
    11581 tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc atatagactg
    11641 ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc taccatttct
    11701 tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt cgatgcaata
    11761 tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc gtcaatcgct
    11821 tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca
    11881 aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa caacagacga
    11941 tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag taaaactgtc
    12001 tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt
    12061 ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa ttttgtctca
    12121 ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa cgtttttcta
    12181 tttttaagcg taatttattc attttttat
//
LOCUS       DARBFD010000082        11794 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000082 DARBFD010000000
VERSION     DARBFD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.082"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(162..341)
                     /locus_tag="SCK08_002165"
     CDS             complement(162..341)
                     /locus_tag="SCK08_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202018.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(722..1429)
                     /locus_tag="SCK08_002166"
     CDS             complement(722..1429)
                     /locus_tag="SCK08_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202019.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1422..1859)
                     /locus_tag="SCK08_002167"
     CDS             complement(1422..1859)
                     /locus_tag="SCK08_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202020.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1874..2125)
                     /locus_tag="SCK08_002168"
     CDS             complement(1874..2125)
                     /locus_tag="SCK08_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202021.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2125..2331)
                     /locus_tag="SCK08_002169"
     CDS             complement(2125..2331)
                     /locus_tag="SCK08_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEG1202022.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2768..3028)
                     /locus_tag="SCK08_002170"
     CDS             complement(2768..3028)
                     /locus_tag="SCK08_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202023.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3103..3306
                     /locus_tag="SCK08_002171"
     CDS             3103..3306
                     /locus_tag="SCK08_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEG1202024.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3447..3932)
                     /gene="ssb"
                     /locus_tag="SCK08_002172"
     CDS             complement(3447..3932)
                     /gene="ssb"
                     /locus_tag="SCK08_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1202025.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3935..4834)
                     /locus_tag="SCK08_002173"
     CDS             complement(3935..4834)
                     /locus_tag="SCK08_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEG1202026.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4847..5440)
                     /locus_tag="SCK08_002174"
     CDS             complement(4847..5440)
                     /locus_tag="SCK08_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEG1202027.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5516..5665)
                     /locus_tag="SCK08_002175"
     CDS             complement(5516..5665)
                     /locus_tag="SCK08_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202028.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5681..7885)
                     /locus_tag="SCK08_002176"
     CDS             complement(5681..7885)
                     /locus_tag="SCK08_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEG1202029.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7896..9356)
                     /locus_tag="SCK08_002177"
     CDS             complement(7896..9356)
                     /locus_tag="SCK08_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEG1202030.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9417..11789)
                     /locus_tag="SCK08_002178"
     CDS             complement(9417..11789)
                     /locus_tag="SCK08_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202031.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2931 a         2465 c         1982 g         4416 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaagtgcta tcacttttag tttttaggat aaaaagtgac gtcacttttt gagtgctggt
      181 acctcaaatc ctgctttttc ttgggcaatc aataaatcta acgcaataca cataatcctg
      241 gattcacttt tgccgtactt ctgctttagt tgctgcaatt ctttttgtct cttttcatcc
      301 aacgtcacgg gtaaccgttt atttcctatt gcaattggca ttagtactct ctcctttcat
      361 cgaaataacc ctattaaaaa tatggctgca atcaacttga atattagact cacaagggtt
      421 aacacaaagc gccaaaagat caaacaaaat accagtattc ctaagaatac tacgaagttt
      481 aaatgtagag ctgcaccatg acgcataagt atctcgccaa caaacagtac gaacaataga
      541 ggaatgattt tattcatatg gatctccttc tttctcttag gttatcgggc tttgcccgct
      601 tatttcgcac cacctaagcg gcgggggcgg ttggatcgat tggtgcgaaa taagaaccat
      661 tcccttaatt tcaaggtttt ttgttcttat ttcacacatc ctcacttttc ggtataaatg
      721 gttattgacc ttttttcctt tcaaatcgtt gatatggcat tcgtttacgg tcaagttcat
      781 tttggatttc ttctgtatcg tcgtaagtgt aggtgtctaa aaaatcagtt aaagtcgtgt
      841 accagtcgtc gcctgggtta tctacttctt ctacatattc cctcaaaaat gcttcgggta
      901 cagtgagttc ataatccatt cccattaaca cgatagattc cgtcccacac gtcaaaaatc
      961 tagtaaatac aaggtaataa tcgtctaccg tcgcatcttt gctgtttagc tcttccattt
     1021 cttcttccat taattccatg ctctcaaaaa ttgaataata ttctgattct gagtaatagt
     1081 gtttcctgca aatcgtgtag ctgacaaagt tttcgaccag ctcactcctt tctaggtaaa
     1141 cattcatgat atatttttct gtttctagtc gtagaagctc cgctcctttt tttaatgtgt
     1201 caggacgacg tttatacacg tctaaacgat tatggctgac tgtaaccttc gtttgactcg
     1261 tttcactttc catatacaac tgttcttcca ctctttgaat aaagtacttc atttcttcca
     1321 tacaactaaa gtctttttga atccgtctga ttacttcatt caagtgttct ttgtttgtta
     1381 aaaattgcga aacatcttct gttgttagct gataaaccac cttattcatt atctttctcc
     1441 tccgattcaa tcgtataaaa tgcaccattt ttttcgatat tttcaaaagt atctagccag
     1501 tcgctagcca ttgctttagc tttcttaatc tgtgcgaatt ttgcataagt cagttctaag
     1561 gcttgatatt cggaaggttt gatagtgctt ggtttgacac ctacttccaa gcatagtaag
     1621 gcttggaaaa accattttcc attttgtttt ttcagttctt ccagtgttcg atagtacgtc
     1681 gcaatgagat tcatttcgac acctgattca atcatttctt ccactgcgac tgacaatttt
     1741 tccttgtaat gttttttggg tacacgtgtt tcatggtata ggaacgtcat atcctccatt
     1801 ttttgtgcca ttttatccaa aggtaatttg acaaattcgt catagtaatt ttgagccata
     1861 ttttttctcc tttttagttc tctaatttgg ttaaccaata ttcgatttca taagtttcca
     1921 tgttttcttg ataatgcagt tcatatcctg caatcttcac taggtcttca aatagcatag
     1981 tcggtgcatc ctctactaaa ggggtttcta tcgtccagta ccgcggaata agtaaagctt
     2041 ctgctacttt ttcagaaatg acttctttgt ccaccaaaat ttgaacgact tcttttacag
     2101 tcaataattt gtgttgtgtt ttcatcaccg tttctttact ttctcatcaa tcaaaacata
     2161 atcgccataa gacaaggtat attttttccc gcatgttgga cattgataca aacgatagct
     2221 tttgatacat ttcgctcttt ctccacaatc gcattcgaca gtatgtggtg catgatagtt
     2281 tgggaataat tcgctgaagg gaacacccca ataatcaaat ttctttcgca tgattgattc
     2341 tgcctcctct taggaaaggt tttatttcct ttcgttagtt ctttctttat tgatttgttg
     2401 gccagaatga atcaacgaaa agcgacaagg acaagggcgc gcagcgtgcc atttaccctt
     2461 gtccttgtcg ctttttggtg ataaaaggca acttgttgcc ggtctggcaa atcaataaag
     2521 agatagcgaa caggaacgaa ccttttggtg agaggaagtt ttttctattt tgtcttgctt
     2581 tgcaagacac catatcttag tgaacgtgca acgttcactt cctttcttct cgaacggcgg
     2641 tggcggttgg agtgagagaa aggtttttca attgagaagg cacttgtgtc ttcctaaaaa
     2701 ggtcttttcc tgtttctatg taaaacaaaa agagctgcaa tcctcattta ggactgcagc
     2761 tcttctttta tatgcccatc gatcgcgtaa aatgatcggg gtttctaatt tgcatcgatt
     2821 caatgactaa ttgattccgt ttattgaaat gacctgtgac ggcaatattg tattttccat
     2881 catccataat taacaattta tcgataattt caatatctgc aactacacaa ttaatcgatt
     2941 catgaattgt ttgtaaagta aagcgaacca ataatggttg tgtttttatt actcggattt
     3001 tatcaagcaa accaacatat ttgttcatca cgatcacctc gctaaaatta tagcaaaaac
     3061 agctaaaatt gagaattttt attcgatttt agatatgcta taatgatcaa gacagtaaag
     3121 tttgtgacgg tggctatccg attgaaagtg aggtggtgcg tatgtgcgta cttccaaaat
     3181 cttacagaaa ggagaagcct tttgcctgtt gaaacggcgc taggattaat gattagtttc
     3241 ggttctttga tcgcaacaat catttttgga atcttagcag ttgtaaacaa caatcaaaaa
     3301 aaataaccgt cagctttagc cggtcgacga ttatttttca aaaataatat taagctggtc
     3361 accgtcttaa acggtgctgt taggggagtt gttagcgcaa ctcctctttt cgtatccatt
     3421 ataacaactt tattgatctt ttacaatcag aatggtaaat catcatcact aatatcgatt
     3481 gattgtccgc caaatggatt ttgtgaagcg ttcaatccat tgttgttagg cgtagctaca
     3541 tcccgttgtt caaaggtggt agggcttgga ctgctggacg attgatcagc gtgcgcacgg
     3601 ctttctgtcg ctgctttact ttctaatagt tggaaattat cggccacaac ttctgtcaca
     3661 tacacacgtt gtccttgttg gttatcgtag gttcgggttt gaatccgtcc aacaacgccg
     3721 atcaaactgc cctttttagc taatgtcgcc atcgtttctg ctggtttgcg ccaaatcaca
     3781 cagttaataa aatcagcttc tcgattgccg ttttgattcg tgaaatttcg gttgacagct
     3841 agggtaaaag tcgctactgc agtaccactc gcggtataac gtaaatctgg atctttggtt
     3901 aagcgtccta ctaaagttac attattgatc atctttatct ctccatttct tgattcattt
     3961 gttgtgatct tttaaactgc cgttttcttt cttgattttc aattttgatc acacttttca
     4021 ttaggtgatc aatttcgttg actgtatctg taaatgaaat ggaattcgca aacgtgttaa
     4081 ctgagcgata tttttcccac tgcttttcca aatagtcatt tcctctgatc ttatccattt
     4141 ctctttcata ataaaaggac aacggttttg tatccccacg ttttgtaaat gtatttcgaa
     4201 cacttttgta taactctttc gtatcgatat tttctagttt cggaatggta aataagtcgt
     4261 agtaatcttt tgctctcgaa ttatctgtgc caaagctgaa cgtagcactt agcttttctg
     4321 caataatttg ttctgttgga taagctggaa tctccacttt tttatcttca aacattaatt
     4381 ggtgatagtg tgtatctact tcaggataaa tagaatctcc tgttgtcacg tccattttaa
     4441 acggtacacg tactttctct aaattagcca ccaatttaat ttgaaatcca ggataaaaat
     4501 cggcttctct tgtttccttc attccttgaa tagagaaaac aatgccttct tttgtgggtt
     4561 ttctagttaa atcattaaaa atgctcttca atttttcttc agttaatttg ctatttctaa
     4621 atgtcgtatc gatatcaatt gttgtccttc tatcgattcc atattttgat cctaataaaa
     4681 agccgccttt caaaatgaag tcatttttgt actttgattc agaaatttgt tcaattagac
     4741 gatccatcat aaaatgccgt tgcactaact gtgcatttaa tcctgtactc tttgctaaat
     4801 ttcttgtttt tgctgcaaat tgtgtcggtg tcatcttttt cctccatcaa tataagcttt
     4861 gcatgtggta tttcatttct ttttctacat gtagttttgt catgtaatct cttaatttac
     4921 ttggatctct caatgggtct tccatgtact cttttaatgc ttctaatttc atttcatatt
     4981 caaattcata tctcggattc caaatatcac aaagtgtacg ctctttatca tacatagtaa
     5041 taggatttcc ttgaaaggaa gttccctcta cttttcctag ttcataaact tctttcttca
     5101 cgaaatgaaa ttgtaactgc tgctcttttg taattgaacg atcaacgtta tagccatggg
     5161 gaaaagttaa gtgaatgaat ttcggcatat tcgtagaaag ctggtaaata tctaaagtcg
     5221 tttcatgaga gaagattccc ctggaaaatc gttgctgaag ccaaaataaa tcatctattc
     5281 catgaccatc ctctacataa taaccacgat caactttgat caattttcct tgattcacca
     5341 atctttgtac agtggttgct gcgattccaa atttcctggc ttccttcata gataaatttc
     5401 catcgcgaat agccaacaat tctttcagtt tatcttgcat ttacttcccc tcccttcgtt
     5461 acaaatacac tacatcaaat atatcatagt agtttatttg taacaagatt aacgtctatc
     5521 gatctaattc gatttctttt tgaatgcgct gctggttctt taattgttgc agtcttcttt
     5581 tatttgcacg taatttctct tgttctaaac gaatttttgc tttttgattt tcgatttctt
     5641 gttgtttgtg tgttgtattt cgcataggat aaccccttca ttattttgta gtgcggcgtg
     5701 ttcttttctt atagttcgtt tttttctttt cagaaaattc aaagcctgtt gtaaagtctt
     5761 cttttaaaac gatcgttgct tcaaacgaac ctttctgacc ttttagtcct ctgactgatt
     5821 ggcttgttcg gccgttttga atgatctctt tcagttgtgt ggctgtgagt ttctttttgg
     5881 caatcgttgt ccataattta aaatcacagg cttcagacgt acatgccgcc acattttgat
     5941 aaagcaagac ttcttgttcc cgacattttg gacaaacacc taaatgacgc gttgcttcct
     6001 gtttttcact ggctgctttc acctcttgta acgttgatcc aaaatctgtt gactggattt
     6061 gtcccggtac gacttccaac aagtgagaga caaatttttg gatattagct aagaaagtag
     6121 cttgtgttcc tttctgctca ccaatcttta ataagtaact ttcccatttg gcggtcattt
     6181 cggcacttgt tagcagcggc tctttttcca cggcttggca aagtaaaatc cctttatttg
     6241 tcaccacaag cttatttttt tctaccttga tatattcctt ttgtttcaaa gtttcaataa
     6301 tatttgctcg tgttgcttct gtaccgattc cttctacttc tttcaatatg gattgtgctt
     6361 cttcgctatc cacggttttt ccagctgttt tcatcgctgt gatcaacgta ccttcggtat
     6421 aaggttgtgg tggcttcgtt tctttttcaa ggacttgtag atctaccgtc accgcttctc
     6481 cttttgtcac tttaggaagt agcggttctt cttccttttc ttttctgtcg ctctctttgc
     6541 caaacagctt tttccaacct agatctactg gtacttttcc gactgtcttt agctgaagct
     6601 gctgaacttg cgtcaagatc gttgtttctt cataggtata cttttccgca aacatggcca
     6661 ccgttgtttt taacacttgt tcataaatag cctgctgcag tggacttaat tgttcaaaag
     6721 ccgctgccgt catgaccgtc tttgtaggaa tgatcgcgta atgttcctgg actttcttat
     6781 tattcacata tcgactattc ggtgtatgaa taggagtagg aatggcttcg gcctttaaaa
     6841 agtgtttgta ttcgtctaag tgatccaaca gatacgcata ctccccttct gtaatataag
     6901 gggtatctgt tcgtgggtag cttagatatt tcccttcata caacccttgc atggcttcca
     6961 aagtatcttt ggcactagct ttcatgagct gattcatttt agattgaagg ctggatagag
     7021 aaaaaagacg tggacttgcc gcactttttt tctcggtttc aacttggtgg atcgtacctg
     7081 gctgttttcc caattgaacc cccttttctt ttaaataatc ttcaaatgct tcttgcgtcg
     7141 cttgggtttc attcggatca agcttcgcat caaatgcgcc attttgagcg attacttggg
     7201 cttttccttc aaaatatggt tctttttgga agtttctaat cttttcctgt aattgataaa
     7261 tcatgtaaag cgttggggtt tggactctgc ctaaggaaaa acttcctggt atgccttttt
     7321 gctgcaagag taaactatac agcgggctac cgttcatgcc gatcaaccaa tcggcgatct
     7381 ggcgtgcttg tgcttcttca aattttgggt aggtctcttc tgcatctcgt aactgctgga
     7441 atccctgata aatggcttct ttttctaagg agttgatcca aagtctttta aatatctttc
     7501 catgatcggc gcctgcttgt tgaatgatcg tccaagctat cagttcccct tcacgatcgc
     7561 tatctgtcgc aataataatg gtgtcggccg attgaagttg ctgtttcacc accttgaatt
     7621 gctttttctt atctttagga acatgatagt gataatgtgt agggaaaata ggcaaatgct
     7681 ctaatgacca ttgtttccaa ttttcatcgt acatatcggg cgaatctaac tcgactaaat
     7741 gaccaaaacc atacgtgatc acagtttctc ctgaaaacaa tcgatcttgg atctcaaaat
     7801 acccatcttt tcgggtagct ttagaaaagc tatcggcata agccttcgct tgtgacggtt
     7861 tttctgctaa aataacggtt ttcatcatga cgatttcact ccctttcttg ctgtctacct
     7921 tgttgaaaca acatggcacg tttttgcttt ctgatctctt gttggtagat ttcatgcttc
     7981 aattgatcat tccaatcctt ttttacttca ggtacaaatt catgttcatg atttttatag
     8041 cctttgtagt attgttctgc tttataagag aaaagaatct gagcattaat atcccctttt
     8101 tcttgataaa aacgatcaaa atcgtacgta ccatccacgc gttcacaagc ttttaattct
     8161 tttgcgactt ctctacaaat atctttcaaa ttaatagctt ctattgaatc gttaacagaa
     8221 agctgctgac caaaagacgc atttactttg tgagcggaaa ccaattggtt cgttaattgc
     8281 agattgtttt ctgtcttgtg tatggccata atagcttctg gtgtgatctt ttcttctttt
     8341 gcgacttctt cataaatgac tttgtattct tttggcactt gccaatcatt agcgattcct
     8401 cgttcacaac gtactttctg attcgtaaat ggatcaactg cgcccattaa ttgttctttt
     8461 tcgtagaaaa tatgtcctgc acggtcatta tccacacaaa tctcgatgga ttgaggaaag
     8521 gcttgtttct ttctcaattc agatactgcc tcaccaaaat aatgactgat cacatggtgt
     8581 tttaaccctt ccatcgaaac taaccacgta tcgtttaact gatcacgatt tagtgcagca
     8641 tagctaagta aatcaatact actctcaaaa aattttaact gcttagggtc accgatcttt
     8701 aggttaaagc catgattcgc tgtcgaattt ttatctatat gcttatatgt gcctctctca
     8761 ccaaaacgtt cataatcaat gaacgttcct tgataactcg cccctactgt ctcatttgtc
     8821 tgtggatctt tccataaaaa ggcgaccgca tgatctaaac gatcgtctcg aactaagaaa
     8881 ttttgccgtt catgttttgg ctttagttcg gcaatcaacc cttctttttc tgcataatca
     8941 atcagccact ctggtagttt tcgttcttcc ttcagataat tccttccttt ttctgtgagt
     9001 ggttgataat caacggggtg atctttgtac ttttctgtct caaaatgaag attttccact
     9061 cgttctgttt tgtcttcatt ctttagaata aaatcagtgg ctgctttaaa ttgttgctta
     9121 gggtcttgga tctctttgtc tatgaaaaaa agtttagcta attcaataat gtcgccacta
     9181 atattttgac tgttccaata aaaacgttgt tttcttcgat caaaaacaaa tgaatcatgg
     9241 tcttctaagc gatagtctcg tcctttattg actacagcca ttccattgtt tcgagcaaaa
     9301 tccactatat cgatctgttt acatcgatca ataagactca tttttttctc ggtcatttta
     9361 aaacctccca aaagaaaaaa gactatccgt ttttcagata gtctttttta cttgttttat
     9421 acacgttctt tttgtgacaa ttgttgttca taatagattt cattttttcg ttttgccact
     9481 tctgacactg tctcaagtga aaatcggaat gaatttccta gcgcaaattc ttctgaaggt
     9541 aacgccgttt ggatctcttt tccataactt tttcctttta aaattacttg attatgctgt
     9601 ggatcttcaa taaacgccgc atccttcaaa tgtaacgttc gacccgtatc ttcttccatt
     9661 actttcccgt ctttcttgtc gatccaaagt gaaccaacag gaacaatatt cttttgttct
     9721 gccgtttcta cagtattttt atgctcctgc ttcacttctg ctggttggat ctctggttct
     9781 ctttctctgt attgattgat ttcattagaa atggtttgaa tgaattcatt tgcgacacgg
     9841 ctgacatcat tcatgatttt cccacgttcc gcagcttcct tatcatccaa cgtctgacca
     9901 ttcttcgtcc atgctgcaat ataaggaata gctttctccg aagtgtccat tccaaaatgt
     9961 ttgcacacaa tataagaagt catttctgcc tgcaattctt tagttgcggt atctaattct
    10021 gaaaatttgt gcattcttgc atgggctaat tcatgaatac ttgtagctaa atgctgcgta
    10081 ggagtatttc gagtattcat gacgatttct tcttcgccag taaattcgtt acgaacatat
    10141 gccccccgtg cttgtcctaa ttctctttga tagacttcac tttctgtcat atctcgaatt
    10201 tgtattccaa tattgttggc taccgcttga atcccagctt gaagttctga ctgtcctttt
    10261 ttatccaatt gaaaatcaaa gacacggttc ggaaagattt tcggatagtc ttcaggtttt
    10321 aactgtgttt gtgtgatatc aaacactttt tcaatcgtgt agtaaggttt tttgatcact
    10381 tcgatttgac cactttcgat tcgtttttta tcttctggtg tagcttctgt ttctcgaaca
    10441 aagccacgtc ctttctcata aaatcctttg accgtttttc tgacaaagat cttgttccct
    10501 tgttcccctt ttttgatata gattccttct tttttcaacg cagcacgacc taaacaagcc
    10561 gttgcacctg gaaattgttc ctcaatcaat aattgatttc taaaggatcg ttctggaaaa
    10621 cgagacataa aattcaaaag ttctttcacg tcttcaggag aatgggtatg ctttttcacc
    10681 gcatccagtg cctgttgcga aagggatttg acttcttgct taatctcttc tgccgttcgt
    10741 ttttgatagc ccttttgttt tgtttttgtt tctttcgact gcatttgttc tttcgaaaca
    10801 ggttcatttt tgaccgtttg tttttgttcc ttttcaggta cttgttcgca caattttgct
    10861 aactctaaat agatgggttg attactgttg acgccatccc ctagatccat tcggatctga
    10921 tcgactactt ctccgttctt cacttcttca aaatagaatt tataataacc tgcttctgcg
    10981 gcgtattggt tatccaacgt tttgagttga tcgatcaatt ctaatgttac gatctcccca
    11041 ccataacttt tcaatccttt ttctggatca ttttcgttga attctactcg atagaaatga
    11101 tcgttttctt tctccttcaa agaatcagtt ggcgcagcct cttcaagtaa ttcacgaaca
    11161 gctgcttttt cctcttccga cgtggcttgg tatttttttg attcctcaaa tcccccgttt
    11221 tcattcagtt ctaaagcaac ttttcgatag tctttcaaaa aataaatggt tggatttttt
    11281 ttccattgcg tcactcgtcc gctgtcaata attgcttgta cttcaggaag aagattttct
    11341 tttccttttt ctgatctttc tttcagctgt tctaatgttt ccaatttccg ttggtatttt
    11401 cggatcgtag cggctgaaaa agtcgattca cctttttcaa agcgttcgat ttcttctttg
    11461 attcttggaa tattttcgat tgtcatatcc aggccgccgt acttgttata gcccatttcc
    11521 tttagctctt cttgtctttc aagtttttct acctgttttt cttgttgctt gatctcttct
    11581 ctcagactat gcactttgtt ttctaaacgt tcttgttgcc taaaccaaga atgcccattt
    11641 ctcttatcgt tcattggctg accgttggtc tttctctggt gttcaaacat cttctcaatt
    11701 tgttcgttta attcattttg caatcgtact aattttcttt tggattttcc caagcgacta
    11761 ttgtctagtg gttctttttc ttctgccatg tccg
//
LOCUS       DARBFD010000083        11568 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000083 DARBFD010000000
VERSION     DARBFD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.084"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(105..629)
                     /locus_tag="SCK08_002179"
     CDS             complement(105..629)
                     /locus_tag="SCK08_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1202032.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SCK08_002180"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SCK08_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEG1202033.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SCK08_002181"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SCK08_002181"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEG1202034.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="SCK08_002182"
     CDS             complement(2351..3031)
                     /locus_tag="SCK08_002182"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1202035.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="SCK08_002183"
     CDS             3157..4026
                     /locus_tag="SCK08_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1202036.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="SCK08_002184"
     CDS             4052..4567
                     /locus_tag="SCK08_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202037.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="SCK08_002185"
     CDS             complement(4614..5207)
                     /locus_tag="SCK08_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1202038.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="SCK08_002186"
     CDS             complement(5232..5411)
                     /locus_tag="SCK08_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202039.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="SCK08_002187"
     CDS             complement(5488..5997)
                     /locus_tag="SCK08_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202040.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="SCK08_002188"
     CDS             complement(6093..6473)
                     /locus_tag="SCK08_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1202041.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="SCK08_002189"
     CDS             6803..7552
                     /locus_tag="SCK08_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEG1202042.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="SCK08_002190"
     CDS             complement(7736..9013)
                     /locus_tag="SCK08_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202043.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SCK08_002191"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SCK08_002191"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEG1202044.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="SCK08_002192"
     CDS             complement(9935..10879)
                     /locus_tag="SCK08_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEG1202045.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="SCK08_002193"
     CDS             complement(10881..11567)
                     /locus_tag="SCK08_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEG1202046.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3291 a         2456 c         2043 g         3778 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccata
//
LOCUS       DARBFD010000084        11560 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000084 DARBFD010000000
VERSION     DARBFD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.085"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            368..862
                     /locus_tag="SCK08_002194"
     CDS             368..862
                     /locus_tag="SCK08_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202047.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(1024..2196)
                     /locus_tag="SCK08_002195"
     CDS             complement(1024..2196)
                     /locus_tag="SCK08_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1202048.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2402..3217)
                     /locus_tag="SCK08_002196"
     CDS             complement(2402..3217)
                     /locus_tag="SCK08_002196"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1202049.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3367..3813)
                     /locus_tag="SCK08_002197"
     CDS             complement(3367..3813)
                     /locus_tag="SCK08_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1202050.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3810..4814)
                     /locus_tag="SCK08_002198"
     CDS             complement(3810..4814)
                     /locus_tag="SCK08_002198"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEG1202051.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(4854..5183)
                     /locus_tag="SCK08_002199"
     CDS             complement(4854..5183)
                     /locus_tag="SCK08_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1202052.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(5309..7636)
                     /locus_tag="SCK08_002200"
     CDS             complement(5309..7636)
                     /locus_tag="SCK08_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1202053.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(8479..8772)
                     /locus_tag="SCK08_002201"
     CDS             complement(8479..8772)
                     /locus_tag="SCK08_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEG1202054.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(9175..10353)
                     /locus_tag="SCK08_002202"
     CDS             complement(9175..10353)
                     /locus_tag="SCK08_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1202055.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(10457..10771)
                     /locus_tag="SCK08_002203"
     CDS             complement(10457..10771)
                     /locus_tag="SCK08_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1202056.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            10878..11096
                     /locus_tag="SCK08_002204"
     CDS             10878..11096
                     /locus_tag="SCK08_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202057.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(11091..11486)
                     /locus_tag="SCK08_002205"
                     /pseudo
     CDS             complement(11091..11486)
                     /locus_tag="SCK08_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         3347 a         2262 c         2018 g         3933 t
ORIGIN      
        1 aacagaacta tagcaaaaaa tcccgtgtat caacacggga ttagattaag tttcctcaat
       61 cactattcat aacacaatta ttataacaag taagtaaaca attgaaaagg acatttattg
      121 aaaacggttg ttttttactt aaggaggaaa tttagtgaaa aaattagttg cgttgttttt
      181 ggtcgcagga agtttgttga gtgggtgtgg tgctactacg tctactactc aaagcacaag
      241 ttcagctcag gcttccagca gcagtaccac gatctccgaa agtacggcag attcatcgaa
      301 agaacaaatt gaaaagaaaa aagaagaaga gaaacaaaaa gcggaaaaag ctgcactaga
      361 aaaaaaagtg acagaagcgg aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga
      421 aaactatgca actgcttcag cagctatcca agcgctacct gaaaacaaac aagagttgac
      481 aagccgtctt gctgcaattg attccacaat caaagcaaaa gaagcagaag aagaacggaa
      541 aaagcaagaa gaaattgcac gacaacaagc agaacaagcg caacaagaag caatcgcaca
      601 gcaacaagca gaacaggcga ggcaagaagc aatcgcacag caacaagcag aacaggcgcg
      661 acagcaagca gcagctgaac aggcttctca gcaggcacaa gccgcagctc ctgagcaaaa
      721 taatgaacaa actgtctatg tcacaccgac cggttcaaaa taccatacac ataaatgtgg
      781 aaatggtacg tatacccctg ccactttaag cgaagctcaa gctcgtggtt taaccccttg
      841 ctccaaatgt tatggaaact aattcaaaag ttatactata ataataaaat tcttctgaaa
      901 cataaaaaaa tagaagctgg cgttccagat agtaaaccaa aaatagcagt ttacacgtcc
      961 atttcgtcag cttctctctt ttttcattta gtctgttccc tctgcttttt tggacatctt
     1021 cattcattct ttctcaatca aatagtacac ctctttttct tttcgcagtt tatatcttcc
     1081 ttttttcaat ttcattgaaa actgtttcga atgtttatgt tttacatgga ttttaagcag
     1141 gagctcctca tgttgcaagc gatagttcct gccttttaaa tcagaaaatc tcactagtcc
     1201 gtattcatca gaagctttca caaatggaac tttttctcta gtaatatagt gtttgccgtt
     1261 gagcgttttt acagaaaaga ccattccttc gattggtcgt ccgtttgtgt tctttacata
     1321 aaaagataaa ggatagtttc gcttagcgat ggccaaagaa tctaaataag cgatgagtag
     1381 ccaaactaag ccgattgatc caagtagtat accgataaac caaataaaaa atttccattt
     1441 tttccattga tcaattcctt ggatcgcttt ttttgcctct tttgcttgat aaggaatacg
     1501 atgccccgtc acaagcagtc gatgtgtatt gaccatataa ggagtgcagg taagcaaagt
     1561 cacgagatct ctgccttttt ggatacccaa agaatctact tcgtcaggta agaccacttg
     1621 gattttttct acttgatacg ccaatgtctt tccattgatc tggatgaaaa actggtctcc
     1681 ttttttcaac ttaggcaaat ctgtaaataa ttttgcttct gggagtccac gatgcccaga
     1741 aagaacggca tgggtgtttt tcccgccagt cggataagaa cttccttcca ataatgacgt
     1801 tcctttttgc aaaaatattt ccgttgtctg atcgaatata ggtaagctga cattgatttt
     1861 tgggatttcg atgactccaa gcatatgttc cataaaaaag gcattactag gtaaatcttt
     1921 taatgctttt gcatcaactg cttggttgaa actgcctatc cctggtgata cgttttgctc
     1981 tgccaactgt tggtttttct ttgtcatttt ttcctgctgt ttttttattt cctctgaatt
     2041 cttattcgat gcttgctttt gataatggat gatcatttgc tgggcaagaa aatcattcag
     2101 tgaatcttct acaaaaggat aagaaaagat cccgatccca acgactaaaa tgattgccat
     2161 gaagatgtcc agtattctta ttctgctttt attcatcatg tccacctatt ttctaatatg
     2221 tatatgaaga ggttgtgaaa tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa
     2281 atagttcctc ctattttgcc gtttgttgag cgaataccaa gagaccgacc acttgtcaca
     2341 acctttctcc ccatttgaat cactatcgtt tatttatcgt atgatttttg tttccaatag
     2401 tttaagcttc tgtttcttga tggcgttttt tgaaatagat cactgctcct ccaactaaag
     2461 cagtacctac caagatgaat acgatgatcc ccataccacc agttgaaggc aagattcctt
     2521 taggttggtt atcgatcgtt gtttcatcag aagttgttgg atcatcatct actgtaaatt
     2581 cagtgtacgc agcttcattc ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa
     2641 ggcagtatgc cccttgtttc agcccttcga ttgaaaattt cccttctgaa tcagaaatca
     2701 atttcacttc ttcccagtca gaacctgtga cttcatctga ccaagcatat gggtattctc
     2761 ctgaggcaag gtttgctatt tttttcatta atttaacagt tggatctttt gctcttagaa
     2821 tagaaaattc tgcatcggct aatttttctt gcgtgttcac atcttgtttg atgaagtgga
     2881 aatcaccgta gttttggaca ttttctacag taatcgtgtt atccgtagtt gtattttccc
     2941 gattgactac accgtttgga ttcccaagat tggcttcatc tgcgccatgg atgataccgg
     3001 ttgtttgttg caattttccg ttttttacta taaaagtagt ttctttttgt ggattggtta
     3061 attgataacc atctggtgct tttgtttcaa ctaaagtata attgccgtct attaatccat
     3121 tgacactgat ggatccttca gtatcagttg tgaatgtcaa cgctgactca gcaggatcat
     3181 agagagcgac accagtgttt tcatcaaaac cggtcacgaa ttgattctct gaatttttga
     3241 tctggaattg cgcccctgct aacttatcta cccctgaaaa gttcccagct ttctggattt
     3301 tgatattgct ttctttgatg acatttttag catataggtg aatcgtgtct aaaggtttgt
     3361 cagggtctag ggtaagctta acacgatatt tggcgacgtt tcgacaccgg gctttggtgt
     3421 ttctacaaag acgtatacag catctttccc atcagattta cctggaagag tcaggttagc
     3481 taatccttca ccagaagtcg ttacacttgc gaagggtgta gcgtctgctg gaactgctgt
     3541 gtctttactt ggatagtacg tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa
     3601 cggttcgcta ttcggaaatg tcaattccgt tccgttgttt atttgatttt ctggtgctga
     3661 agtgaatttc aatttatgca aattaaccgt aacattttct ggtgtaggtg atacggctgc
     3721 agaaacctct gcagttccta ggccgagaac gaaaggaagc agcatgatag cagttcctaa
     3781 aaacgtccat atttttgttt tgatgttttt cattttattt ccctcctatt gtttcctttt
     3841 tgttgttttt tataaaacca agctgtccct gttgaaagga ctagcaataa ccctaaaatt
     3901 gagaaacctc caagcatctc ttcgcttgtt tttggaaagc ttcctccatt tttttcattt
     3961 gatggaccat ttgtctgcat ctgaactggc gtttttccat tattcgtcgg atttgctttt
     4021 ggttcgttga tgatttccaa tggtatagga tgaagcggat cataactgcc tttttcaact
     4081 tcaaagagaa tggttgattg actcaatata tatcctttag gcggtttaat ctccactagt
     4141 tcgtaacttc caaaagacaa tccatggaca gaaaatgttc catctttact ggaagtaaaa
     4201 atcgttgctc ttgaatcatc tttggctacc caatgattta tcccgttttc ttgcgctaag
     4261 tattggtcat tttttttgac tagaaattga gcatctgcta gtttttgttt cgtttgccga
     4321 tccactttga caaattgttt gcctcctgtt tggatggcaa gatgtgtttc tactgtttga
     4381 ttgtcagtcg tcaaacgtac atcgttatca aatgtcgttt tttcactgtt ttctttcaat
     4441 gtcatttggt aacggatcgt taattttttt ccagcaaacg aactaagatc ttttccagaa
     4501 tctgataaga caaaaccatg ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca
     4561 ccaatcaaaa aatcgatcgt ttctggttct agccataact gaggatcagc tgtatctttg
     4621 atcgtatagg ttcctatctc gttgatgttc tcaggaattg ttacggtgat gagataaggg
     4681 actttatccc catctgcaaa atctgtctgc ttattctcta ctgtttttgt taatgtgagt
     4741 gcagcaggta ttttttgttc gctctttgga tacaagtgga tggtcgtcaa tttatgatta
     4801 gatgaatctg tcacaggaag cacaacgaca aatggtgctg atttttcttt tacttaatct
     4861 gaagtcttta tttccgtaaa tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat
     4921 gcactaaaag aagcaatgcc ctcttcctcg tctactgttt ttgtcacatt ttctgccagt
     4981 attttttcac tatcactttt ttgtgctagt tttctttgtg cgtcttccac actacttcct
     5041 tctgaccgca gcttgtaaaa ttcatccgag acatcatata tagcgaaagt aacgccgttc
     5101 aaccctttta ctccttgaaa gttatccggg atatttattc cgtcgttttg actgatactt
     5161 ggcatcttat cgctttcgaa cattcgatta tgcaaagtaa atgtaatagg tgattctcta
     5221 gctgacacag tcggagaacc tataaaaaag accagtagga taaaaaagag aacagcaaat
     5281 atatccacac gtacattttt tgtttttttc atcgctgttc acctgctttt ttcttacgat
     5341 ggataaaata aaagaagcca gaaaaaataa agatcagtcc taaaaagaca aagggcagta
     5401 gacctatccc acctgtcgaa ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat
     5461 ctgttgcgat aatcgtataa ttattgttta cacttccttc gcctaaacta atcgttacta
     5521 tttgtgatcc aatatctgtc cggctgttaa ctgttagggt ataatccaat gattcgccta
     5581 agacaacacc ggtaacgtgg agatttccaa ccgaaatcga gtgactttgg gcatcataat
     5641 taaaggcagc gtcatcgaca gtaatcactg cgttagcttt atagatggta taagttcctg
     5701 atgtgttggc tatatcagta aatgacaact tgggatttgc tgctttgact ttgtctattc
     5761 cgctattcgt tagtttaatt gtatagctgc cagcattaac catatttttt gctgattgtg
     5821 catctgctgt gtaatccata ctgctaagtg ccacttgtac tggagtaagc gaactagtga
     5881 caccctctgc tagcttgaca tggattgacg ggacacctgc ttttccatca tatgtcttac
     5941 tgccactgag ctctaccgca ccgctgattt gcttaatcgt atagacatca gatgtctgca
     6001 cgatagtaaa taaatagttt gttctggcag ctagtttagc tagtccgctt tttgtcaatt
     6061 gccaagacgt ataggttcca atttcagtag gcgcagttac ttcaactccc ttttcattca
     6121 tcgctttgaa atcagttgga ttccatgtga tttgatcaat actgagatca ttgtttaaaa
     6181 gggatgttga ttctgtttta tcattaaagg catatttaac gatcggtaca acagaaagac
     6241 catcatagta cttactgcca tttgcattga ttgatactgt tgcatttgtc ttataatata
     6301 ggatgacttc agaaatctgt tttccatttg cgtcctttac gattacattt ccatttgtat
     6361 ctaaactagc taaaacactc attgctgatt ggttctttgt ttctatcgct gaaacagctg
     6421 taaaggtata tccgttgata gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt
     6481 aagtaagatt cattatttta gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa
     6541 tagtgattgg tgtttgtttg cctgtatagg tcaccgtatc gacgttactt tctagggctg
     6601 tgaaagaacc gtttgtttgt gcaaaagaat agcccgttac tgctgttgga gtgataacca
     6661 ctttgtcacc cacatggatt ccagataatg tagtcccgta cgttccgatt tttgctgctc
     6721 caccattgac tgtataagaa aaagttcctg tccgattcgc attgacctga accgttgctg
     6781 cttgataggt tggaaaattt gaaacatttc ccttaccatc aacgatctgc ccatttttga
     6841 tcgagtaccc cgtgaatgga atatcatttg ctaactgtat accgattgaa aaagtactca
     6901 tacctcctcc ggcaggaagc cctccccatg ttgagggtgt aaaagaataa cttttatccg
     6961 cattgggtgt aactgaatcg atcgtatttc cagcggtagc cacccccata tagatacttt
     7021 ttgggaatgt gatagtagta ccatttactg tggagccaat aaacagatta tggatatctg
     7081 gacggttatc aataaaagcg attgctttgg ttacgccttg taacgcagtt ttatccgcac
     7141 tcatcgatcc gttaaccgca acaatgactc catctttggc atatatcact gtatatccat
     7201 ctggtatggt atgtccgttg aaattcgcag tggccgcaac agtactaaat gtctggcttc
     7261 tacctgtttc tgaataagct ttctcccctg ctgcttttgc tgctgtgatt tctttttctg
     7321 tcggatcaat cagttgagtc gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat
     7381 tggtgctttc tatggactgt atttcagaat agggtggact atttgtctcc tctgtcgttt
     7441 ggttagcaaa atttgacgtt tctgtataaa cgtttacata aatacttgat aagctcccta
     7501 agaaagtaaa aatcaagatg atcgaaatca taatgggatg taagtatttt cttttagata
     7561 cagctttgag tctagaaaca aaatgttttt gacatatacg ttggatttta ttttgagttt
     7621 tcttaatcat tttcatccct cctctcaata aaaaatcatc aaataatcct ttacacgtta
     7681 ttctaaattt aatttttatt attttattga tgtatagaaa gtttggggct ttttttctct
     7741 ttttattcta aggttatttt agtcttgtcc tttaatacaa agattttatt tttaaataaa
     7801 aaatactctt tttttacgat tattgaaaaa aaaagataaa acttgattaa ttgaagtatt
     7861 gcttaaattt ggaagctatg agggaagaat aaaagagtta ggacgggatt atctactatt
     7921 tttctttgtt ttaaatattt gttatctttg aacataaaag agaaatgtgt cgactgcagt
     7981 gttgtgtccc tcatcacgaa taaacggtgt tcaattagct gatcttgtat tcacttaaaa
     8041 aatggtaaag tacgagttgc tattccccat tttcaatttt ctaacgctca ggacaaaata
     8101 ctgttttcac aacctcattg gaataaaagc atatatattg tttcaagaac gaaatcttgt
     8161 tatcttttta aagggcatct gtccaaaatc taattttagg aggtacgttt tggtatttat
     8221 tcgtacaaat aaaagagaat tatctttatt catgtaacgc atccaattga ttttttcatg
     8281 tacgctcctg gaacgatttt actgacgatt gcttatttaa ctgcgaatcg ctcattagct
     8341 tttatcatag ctatccatat tttgaataac gttcttggct ttgtattata gatacagatg
     8401 acaaaaaaag ttcaagtatg cggtgtagaa ccaatatcgc atacttgaac ttttttcaat
     8461 gagtataaat taaaacttct aattttcaat tcccatcatc tgatatgccc gaccgtctga
     8521 acttcttttg aaaaagccaa atgtgattag ataacgtctt tgttcgacat aatcgccact
     8581 agaaaatacg ttcagcagta tcgaattaac ctctgtttct gtataagtca ctccatggtc
     8641 aaagcgtttc aagacttctt tcaataggat gtatttcttt tttgttgaac ttggtacttg
     8701 ttttactttt ccatcattca tgaatgtgtc taaaactttc atcttttctt gctccgtata
     8761 agtataaacc atcttctttc tccttttgat tctaagaata ctccatttat gttagaatat
     8821 tctggatttt ttattaaagc ggttactctt aagaaagata ttatcatagt gctctttttt
     8881 ttactactac ttataggtag atttattcta atccattatt ctcccctacc aataattaaa
     8941 gaaaaaactg gatcagggtg ttcaaattac atgctaagaa aaaagaaccc ttgaatacca
     9001 agggttcttt ctcagtctga taacaatgat taacgacgga tttctttgat acttgtgaaa
     9061 agtcgttaaa gtattgatat aaaaggattt ttattacctc tgactacata ttgactaatt
     9121 tgttaaaaaa tcgtttttca agaatcctgt aataccagtg ctttcaccgt ttttttaact
     9181 ctctaaagaa ctaaaagcaa tcagaggttt taccttttca tttgacgtta agtggcgata
     9241 aattttcgaa gttgtttggt cagtatggcc taagcgatct ttcaagactt cccagctaca
     9301 gtgttccgct actaagcttg ccattgtatg acgagctaaa tgagtatgaa atggcttatt
     9361 aattcctgag cgattcacgc accgtttgac gtgatcattt acatactctc gacgataagg
     9421 aacaccacgt ttactagaat caagatacac aaacaaatat gtttcttcta taataaacct
     9481 gtaatgttgc ctcatctcac ccaaagcctt aatcaactgt tgtagctttt ccataactag
     9541 aggagttact agaactcgtc tagcagaact aactgtctta gtagtttcaa ttcgaaaatc
     9601 cttactctta cctttaactg ccgtttgctt atagaacaga atttcatttc ttttgaaatc
     9661 tatatctcca ggctgtagag caagcgcctc gctgactcgg cagccagtgt aaaatagaac
     9721 atggaaaagt acagacatcg gtaattcctc atgaacaatg ccataattca aaaaagttct
     9781 caattcatcg acagtaagaa acttgtcctc aatattattt atggcttctt ttaaatcagt
     9841 taccgttaac tgatattttg gcattctcac agacttcatt ggattttcac ttatcaaatt
     9901 ctttctttga caatatttaa aaatcatatt tagaacttga ataagtgatt gtaaactatt
     9961 tttggcatag ccaacatttg ttccattaac gccacattcc tttaaggtaa aaagcaattc
    10021 ttgaaggcac tctggtgtca aatcagacaa ctttttatca ccaatccatc gaacaataat
    10081 ttttaattgt tcctctcgct tatcataggt tatttccttt tgttcattta acgaacggta
    10141 atattggaac cattgctcac ctacaacact aaacttgtca ttgcttgcaa caattttttt
    10201 cttaagcctt tgttgtttct ccatttccag ataagcgagt tgcgcctcac gttttgaatt
    10261 aaagccacgt cttgttgttc gatatgcttg aaagtaccat gtgatcctac cagtagaact
    10321 gatttgcttc ttgaatcgat ctgttttttc catttattga ttcctcctat tttgaaggaa
    10381 cccaacaagc agaaaccctt tggaaatctt tctatctatg tcatcattgt accgcatctt
    10441 agaaagaaaa tgtaacttat aaagatcgaa gtagtcgcaa tttttctcga tagtcattcg
    10501 aatcactctt tctcatccat tctaaaacat catcacgaaa atagtgtcct cgaatcccgt
    10561 accaacaggg aggaaactca ccaagtttta ctaaacggtt cagtgtacta tcacttatct
    10621 gaaatagtcg ctttaaatca gtatttgtta ataccagagg taaatgactg actgcaagca
    10681 atttttccgt gtaatcttcc agtttttttt gtaaaattga atagaactct atttccagag
    10741 aattaacatt tgttttaact tccaactcca tcacccttct ctctcaaaat caaaacgacc
    10801 accacggtac cccttaaaat tgtaaaaaaa cactccagta ctccatcaaa acggactaga
    10861 aaagaggaat ttttaatatg aaacaaggaa ttgcagacat aaaaataatt aaagaaattc
    10921 ttgaaaaatc tacggctaat gcaatagcat tcggaacagg aatcaacttg agtacagtaa
    10981 aaaaacttaa aagtggagag agagctgaag aaaaattaaa tcttgccgat gctattaaaa
    11041 tcacagaatt tggcatgaaa aatatgccaa ccaaaatcga aatatggaaa tcctagctct
    11101 tccaaagcca tttgtcgttg gtaccaaaca ctgccgagac ttttcccaat aatatcgcca
    11161 atttcacgat cattaagacc gatgaagaag gacataaaaa tgatttcttg tctttgacta
    11221 ggtaatgctg caatcgcatt tgccaactcc tcattggaaa taaagatttt cataccaagg
    11281 acaataaaag taccactact attcaaattt ggttccaaat aatagaactc tgtacctaac
    11341 cctttttgtt ccaatatttc cattgatatt tgccgttggc gaaatctagc gtacttcttt
    11401 tgaatattta acgcctcatt gcagactact ttcttacaaa aactgtcaaa cgtatgttga
    11461 atgtgggttt cctctgagtc gcccataata ttctccccct caaaaaattc attcaactcg
    11521 ttttacttct atccctttcc attattacta caatctagga
//
LOCUS       DARBFD010000085        11529 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000085 DARBFD010000000
VERSION     DARBFD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.086"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(131..676)
                     /locus_tag="SCK08_002206"
     CDS             complement(131..676)
                     /locus_tag="SCK08_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1202058.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(688..1380)
                     /locus_tag="SCK08_002207"
     CDS             complement(688..1380)
                     /locus_tag="SCK08_002207"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEG1202059.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1399..1683)
                     /locus_tag="SCK08_002208"
     CDS             complement(1399..1683)
                     /locus_tag="SCK08_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202060.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1696..2259)
                     /locus_tag="SCK08_002209"
     CDS             complement(1696..2259)
                     /locus_tag="SCK08_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1202061.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2487..3149
                     /locus_tag="SCK08_002210"
     CDS             2487..3149
                     /locus_tag="SCK08_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEG1202062.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3273..4469
                     /locus_tag="SCK08_002211"
     CDS             3273..4469
                     /locus_tag="SCK08_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEG1202063.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4570..5946)
                     /locus_tag="SCK08_002212"
     CDS             complement(4570..5946)
                     /locus_tag="SCK08_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1202064.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5983..6849)
                     /locus_tag="SCK08_002213"
     CDS             complement(5983..6849)
                     /locus_tag="SCK08_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEG1202065.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6938..7963)
                     /locus_tag="SCK08_002214"
     CDS             complement(6938..7963)
                     /locus_tag="SCK08_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1202066.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7981..8655)
                     /locus_tag="SCK08_002215"
     CDS             complement(7981..8655)
                     /locus_tag="SCK08_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1202067.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8839..9732)
                     /locus_tag="SCK08_002216"
     CDS             complement(8839..9732)
                     /locus_tag="SCK08_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1202068.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9806..11503)
                     /locus_tag="SCK08_002217"
     CDS             complement(9806..11503)
                     /locus_tag="SCK08_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1202069.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3423 a         2523 c         1976 g         3607 t
ORIGIN      
        1 gatttattaa cgtaaaaaga ataagttttt ttaaaataac gtataaaaaa gagcataaaa
       61 caaagtcgtt tgttttatgc tcttctttta ttattttgtg ctttttattt tatcaaatca
      121 ctttgttttt ttataaaatt tctgcgccta gtgtggactc aaaatgtctc aatgcccatt
      181 catgtccaac gggatcgaaa cttgccacgg catctttgaa aatatggagt ttgtatccta
      241 gattatacgc atcgactgct gtatgcaaca cacagatatc tgtacatacg ccggtcaaat
      301 aaatatctgt gatctggcgt tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg
      361 aataatgccg tttgtctatc caataaacat tttcctcttg ctcatagtct tggtataaac
      421 tttttaaaga gccgtacaat tcccgtccac ccgttcctat cacattatgc ggaggaaata
      481 agcggttctc cggatgaaag gaatcttttg gatcatgcgc atcaatggca aaaacgacaa
      541 aatccttttg atcaataaac ttctttgtat gagcagtcag ctttttttca attacttgtc
      601 ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg
      661 agatcaatgc gttcatctta gtcctcctta tacgagatgt ttgacaaatt cgatcaccat
      721 ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg
      781 atctgctgtg tcgctcattg cacgtacgat caaaaatggg atattgaact gacgagctgt
      841 ttgtccaaca gcggctcctt ccatttcgca agctaatgct tcagggaaat tcgttaagat
      901 ttctttgatt ttatctggcg agtcgacaaa tgtatcacct gtaacgatca atccttcttt
      961 tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc
     1021 ttcataatat agaggcattc ctggtaattg tcctggtttg tagccaaatc ctgttgcatc
     1081 tgcatcaaaa taagctactt tgtctgatat aacgatgtca cctacttgta agccttcacc
     1141 gattccgcca gctgaacctg tattgatcac catgttcacg ccgtattgtt ggatcaacag
     1201 acttgtggta atagatgcta agacttttcc tataccggaa cgtacgacga tcacttcgtg
     1261 gttacctaat gaacctgaaa taaataaagc gcctgcgcgt tcccatgata atggttcgct
     1321 taggttctct ctcaaaattt ttacttcttc ttccattgcg ccgataatac cgattttcat
     1381 ctttttaatt ccttctttct atataaatgc gattgctaaa aaaaccacac ataacattag
     1441 gataacaata ataagagatt tcatcaaaaa agagttccat ttggtcgtct tttcccgttc
     1501 gctgatcctt gttttcgtga ttggtttatt ctttttgctt tctttacgat aaaagctggc
     1561 aatctttttt tcttcttgct gataagctgt ttctgctttt cgctgttttt ttaatgactc
     1621 gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc
     1681 cataagccga tccctctact ttcctttggt caatagttcc cagtattgaa tcgcataaat
     1741 cgtctttgca tcacaaatca actgatcttt catcgcttgt tgcgcctctt caagagttag
     1801 atgatacaac tccagtactt cgtcttcatc ttgtgctaaa ggattctcga ccttctctac
     1861 cccttgtgca tgataaatat gaaggacttc atttgcaaaa cctggtgaaa gatacatcga
     1921 agtaagatgg ctcagcgact tcgcacgata gccagtttct tcttctaatt ctctagctgc
     1981 tgtcatttcg ggattttgtc cttctcctgg atcgattttt ccagctggaa tctccaaaat
     2041 cactttttct aaaggttttc gaaattgttt gacaagaagc agacgatctt gttcgtcaaa
     2101 agcaatgatc ccgacaccac caggatgaaa taccagttct ctcttcgaag ttcctccatc
     2161 tggcaaacga acttcgtcta cagcaacatc aataattttt ccgctataga tttctttgcg
     2221 actgattgtt ttttcttcaa attgctcatt gttcagcata tcttctcctg ctttctttgt
     2281 aatcataact gctttttttt catttttcaa tttttctcaa aaactcctta ttagtttacc
     2341 atagataaac cttagatttt ctctaaatat agaaaatcct tctttagtcc gatactcttt
     2401 ctagtagtcc gtatgctaaa ataagtatgt aatcgaataa cgaaaagaat tcaatactca
     2461 acgataaatg acatggaggg tttattatga taaaacgaaa ttggccgtgg cttctagcat
     2521 tggtcttgct atttctcttt gtccgaaaca atagtttttc ttttgttcta ggacttttat
     2581 tgataggtgt agttttagtt ttgtggggac tctttggttc gcgcaggaaa aagggaaaac
     2641 aagaagaaat tcctgcactg actgacgaac ttgaaactca ctatgaaaaa agcggtatga
     2701 cagcgaatga aatttcattt tttcgacaaa caatgaatca aacaaaaaat gagatcaatc
     2761 aattgcaaat gaatatgcag cagacagcca aacttaaagc aatcgatctc cgccatgatc
     2821 cagtcaaagc agcgaaagct ttattcaaag aattggtgaa agaaccaaac cgactgcatg
     2881 aagcaagtca gtttctttat acgcatctgc cgaatctagt tgatttgaca aataagtata
     2941 atgagatcaa tgaccatgag atcaaaaaca aacagaccta tgaaaaacta gaagaaagcg
     3001 cgcagatcat cgaccagctg tcagctttaa tcgcacaaga ttatcaaaaa ttcgttgctg
     3061 aggatctaga tgatatcgac gtagagatat ctgttgcgaa acagagctta aagcgtgata
     3121 ataaatatga accagaacaa aaggattaac tcttttgttc ataaaagaag actcggaaca
     3181 ttcagatgat gtctggctgt ttcgagtact ttttctaaag tagagacatt catgtaagaa
     3241 tcgagaacag attcaacaag gaggaatttt ttatggatca taaaccgact gaaacaaaag
     3301 atgttacacc agttaacgat acacttgaag atttattgaa caacccgttt tcaactccaa
     3361 cagatacgct gaccacttca caacaagcgg agattgatca gctccaagag caacagacag
     3421 ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga acaagctaaa cagctagcag
     3481 caaaaatcga tgctaatgat gcacagtctg tcatcagcta cggatcggct gcccaagcaa
     3541 aattaagtga attctcacaa tcgatgttga atcatgtgca agcacaagat attggtcctg
     3601 taggagattc cttaactgaa ctgatgtatc gtctccaaga agccaatcct gacgaactgc
     3661 gtgccggaga agggaacatc ttccagcgtg tcttcgggaa ggtcaaacaa tcgatctatg
     3721 aagtgacagc aaaatatcaa aagattggtg cacaaattga caagatttca gtgaaattag
     3781 ataaagaaaa agacggtctg ctaaaagata atctgatgtt agaacagctt taccagaaaa
     3841 acaaagacta ctttgatgca ctgaatattt atatcgctgc tggagaattg aaaatggaag
     3901 aacttcaaac aacgatcatt ccagaagcga tgaaaagagc agaagaaact ggcgatcaga
     3961 tggatgttca gattgccaat gattacacgc aattcctaga tcgtctggat aaacgtacgc
     4021 atgatcttcg cttagctaga cagatcacga tccagcaagc accacaaatc cgtttgatcc
     4081 aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc tatcgctaca gccattccgc
     4141 tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg tcaaaaagat gcagtaactg
     4201 ctcaacgcca agtctctgaa acaacaaacg acttattgaa gaaaaattca gaaatgctga
     4261 aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg aatcgtggat atcgagaccc
     4321 ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga gacattgcgt atccaaaaag
     4381 aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg acacatggaa gaagatctga
     4441 aacaaaaact gttggattta acccaataga tcacctttcg cttgttacta aaacaaacgc
     4501 ccaacaacag tttattacaa ctgttgatgg gcgtttttct tatctttctt ctaacttctc
     4561 cattggagtc tatttctcat tcatcttgct gtgtccatag ccatagccaa aataaatgat
     4621 cagtccaata accattacga taccgaacac gatccatgtc acagtttgca gacgaagcat
     4681 caatacaata caagtcaaga tggagacgat tggcaatagc ggaacgaatg gcactttgaa
     4741 ttctccagct tttggtttgc caaacatttt tctcaacttc agtaagccta gactaacaag
     4801 catcaaatag gcgagcgcca tgatattcgt tagttccgct aatacattca atgggaaaaa
     4861 tccggcaaag aatacagctg ccacaccagc ggcataagtc gcttttttcg gtgtacgatg
     4921 cttttgatcg atttctgtca taaacttagg aagtaagcca tccttgctga cagcaaacag
     4981 taaacgagca agtgaataca tcatcgagat cgttaccgtc agtagcgtta aaattgcccc
     5041 tacagcgatc accgcgccca ctgttccatg tcctacatac ctcattgcaa aagccactgg
     5101 gtcattgact cctagttcag tatacggaac gattcctgtc aagatcagcg tcacgatgat
     5161 gtaaaggaca gtagcaatca gtatcgaacc aatgattcct cttggcacat ctttttgcgg
     5221 gtttttgact tcctcggctg ccattgcaac ggcatcaaat cccaagaaag caaaaaacac
     5281 aagagctgca ccatctagca caccggaaaa accaaaaggc atgaatggct gccagttatc
     5341 aggtttaacg aaaaatattc ctacaatgat aaacaacgcg atgataccaa atttgacata
     5401 taccatggca ttattcaaac gtaatgcttt cttggcttca agcgacacga tgtaagtgac
     5461 gaatatcact acgagcaccg caatcaagtc aatgtatgta ccattttccg gattgtaccc
     5521 gccagttaat gcttgtggaa gttcggcccc taaacttttc aaaaaacctt gcatatagcc
     5581 agaccatccg gaagcaacag aggaaaccgc tagtaaaaat tccccgatca acaaccaccc
     5641 tgtcagccag gcagcaaatt caccaaatac aacatacaga taggcatatg gcccaccaat
     5701 cactggcact cgcgaagcaa attctgcgaa acttagtcca gacaaaatga caacgattgc
     5761 agctaagaca aatgaaagtg acagtgctgg accagcattt tgattggccg caacccctgt
     5821 caccacaaat attcccgtac cgatgatcgc accaattcca agcatgatca aatcagacgt
     5881 ttttagttct tttttcatct cactcgtatg attgagattg atctctttct tacgaaacaa
     5941 atccattgaa gttcctcccc attttaggtt ggaaaacagc gattaaactg ttttcatata
     6001 ccctaaaagg ataccaccta aaatgactaa aaggcaacca acaataacat aaatgagctc
     6061 ttttttcgtt tttctttcgc caaggatgaa aatcccacct aaagtagaga taatgatccc
     6121 catttgtgaa agtgagaagc caacagctaa accaagtgat ttgactgtta gaagcatgca
     6181 gatgtttcct aagccccata ataatccaca gatcatgttt ttccacacaa actgatcgac
     6241 tttaacttta cggaaagcaa agaagcttgc gcctaagatc attccgatac tttgaggtaa
     6301 gataattgac atcgcatcca aattcgccca gttgacgatg atggtatata caccataacc
     6361 aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa tcaagcattt tattatctgt
     6421 atctattttt ccttcgtcat cttgtcgggc agtcaaatac gcaccgctta caagaagagc
     6481 taaagcaata ataccgtata cataatcttt cgtttgtgtc cattcatgga agaaaacggc
     6541 tcccgcaatc gtattcaaaa ttaattgaaa accagtagac aatggtagcc ctacagaaac
     6601 acccatgtac ttcattccat ggaactgtcc attttgtcca acactccaag ctaagccgga
     6661 taaaaatccg atcaagaaaa tccaaggggt gattacagga ctaacaacga aaaagacaac
     6721 taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt tgttgattgg catcgccacc
     6781 aattttacca cttactaaac caatactacc ccatgccaac atagggacta atgctattaa
     6841 aatttccata accaatctcc ttttatttca catgataaga aacttatcag cgaaaacgtt
     6901 aacaacacaa gcactttttt tcgtttctaa cgattttcta acgtttttca ttataaaaat
     6961 ttaagattgg gaaaaataaa gtcattgttg tcccttttcc ttcagttgat tcagccgtca
     7021 aatggatacc taatttttcg gccagatttt ttgctaaata aagtcctaat cctgttgaat
     7081 gtgttttgct aagtcgacca tttctcccag taaatccctt atcaaagatc cgtttcaagt
     7141 cttccgctgg gataccgatc cctgaatctt taactgaaag gctgactcct tgcgcttcct
     7201 tttctatgct cacaatgatg tttccgtgat ccggcgtata tttgatggca ttactaagca
     7261 attggttgaa gataaaagcg atccatttag catcggtcaa aacagtatga tcctcaccaa
     7321 cgatctcgta gcgaatattt ttttggataa aataatttgc ctgactgcgt atcacaggct
     7381 gaatgattcc ttttagtcca tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc
     7441 ttgcataata caaaacttgt tctacgtatt catcgatttt tgataattca ttttctagta
     7501 atgtatactt ttgatcgtca atatcgaact cgatcgaacg aactagcaat ttagaagcgg
     7561 aaagtggcac tttgatttca tggacccatg agtcaatata atctttttgg tcttgttgat
     7621 tactgattgc tgattgcatt acttcttggt gttcacggat cagctgattg atatattctt
     7681 ctaccagttt ttcttcttct gtcctcccac cactcaaata attttgcaaa ggggaagttt
     7741 tttgtatgtc ggctaatttt ccccaccatc ttctcttgct caagtaacca cccagcagat
     7801 aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc aattgtgctc aaatcaactt
     7861 tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc aataaaaaac agccatccca
     7921 agatcaatgt ccactgatct ttcaaatatt taaaaaaatt catcttaacc gctcatcctt
     7981 tcagggaacg atatatccct gtcctacttt tgtttcgata tatccttgga tacctgcctg
     8041 ttcgattttt cggcgtaacc gattgatgtt gactgtcaat gtattgtcat ctacaaatcg
     8101 ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt aagatctttc cattgtgctt
     8161 catcaaaata aataatagac gatattcatt tttgctcaaa tcaatgatct ctccgttgat
     8221 ttccatactt ccgttgtcaa cattcaacgt aatgccattg tggctcatca tttcactaga
     8281 taattctgtg tagttgtaag aacggcgaag taatgcattg atttttgcaa tcagtacatc
     8341 gatttggaaa tgttttgtca caaaatcatc tgcccccatg ttcatagcca tgatcatatc
     8401 catattcgta ttcctgctgg agataaaaat gatcggtact ttcgatactt cacgtatttt
     8461 ttgacaccaa taatagccat ccaaaacggg aagattaata tctagtaata caagctgtgg
     8521 ttcttctctt tcaaagtctg agaacacttg attgaaatcg gtcgttccaa aggtttcaaa
     8581 ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac
     8641 catgattttt gccataaaaa atccctccat ctttcttctt gctactacaa tatcaaagaa
     8701 gcccttgtga aacaagttgg cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa
     8761 ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc agcttaaatt tattatgtta
     8821 ttcttaataa ttggtctatt atctgttatt tttctttcgt tgtttttgtc ttttaaattt
     8881 tcgataacac caataaccga aaagcgttag cactagcaat atacctagaa ggagtataag
     8941 taatcgataa agatggtatt tttttgtctg ttctatcttt gaagagactt cttctgtaaa
     9001 gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt
     9061 gataagtgtg actagatcct gcttttctct aattgctaat gtatttgttt ttgttggaag
     9121 gacaacaatt ttctcaacga cttcataagc gagcatccga ccatacactt caagatagaa
     9181 tcggtcatct tgctccattt cttcaagatc cgtaaagagt tttttctcag caataccgct
     9241 atgggcggta atcacggaat gtgtactctc tcctccagtt ggaaaagagg tgccttgcag
     9301 gactgtcgct cctctatcta acaaaagatc attcgtttca tcaaataaag gcaagctaac
     9361 attgattttc ggaatataga tggctccgat catatgttgt tcgtaatatt cttttcctgg
     9421 attttggaca tttctaaccg cttgctcaaa aggatctttg acttgtccca ttcctgggat
     9481 tactgatgta tgagcaagcg tctgattttt cttttcatgc actgctaatt gttgctcatg
     9541 tttttcttct ttatcgcgtt caagctgctt ttgataattc tcgatccttc tttgatccaa
     9601 ataattgctg agagcatctg ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa
     9661 catcactgcc ataaataatt gtaataaaag actgatttgg gtttgaggtc tattacctat
     9721 tttctttttc attttcttca ccttcttatt taaaaaaact gcagcaacct ttataaaacc
     9781 gattgctgca gcttattttt gttgcttata cttcagactt cattttagat ttacggtacc
     9841 agctgtacgc tccaatcatc aaacttattc cgattagtaa gaatgccaag agcccgttcc
     9901 caccagtagc aggaagtgac ccgcgtttca tattcggtac tctttctggc cctggatttt
     9961 ctactcctac gatctgaatt tcttcattat agcttccata agagatgata aattcagtaa
    10021 aggctgcctc acctgttgga agtacataac cttctggcgc catggtctct ttcatttgat
    10081 aggttccata atctaaccct tgaacctcaa agtatccatt tccatctgcg acaaattctg
    10141 ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc tgattcataa acagctaaag
    10201 gcgactcccc tttataaaat attgcgtatt cagaacggtc ttcattccac aattcgaatc
    10261 ttgcatcttt aagcaattca ctgcttgatg catcatattt ttcaaacttt cttccacctg
    10321 ttgtgacttt ctctggcggc tctactcctt cttcataatc aaattcatca tttcgtccga
    10381 tttcaataac cgctgtgttg ttatggaaat catccgcttc tagttctgtg gaaaccttca
    10441 aattatacgt gatcgttaaa tctcctccac gtgcatctgc taatctacct aaagtatcta
    10501 ctttggctac tccattttct tctgtcaaaa tcgtcacgac aaacccgttg cttactactt
    10561 ctactatata atcttgtcct tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct
    10621 gatcataata agttagacca actgtaggcg tatctgtgat tttgtaattt ttgataacag
    10681 cgcttccgtt ttcaaggaca gaatcgatga aataaggaat aggtgctgtg atcgtgtaag
    10741 aagccgtttt tcctctttct agatcaatat aagaaatcgt ttcttgttct ccagcttcat
    10801 taataatcgt tacttgtcta aggtcgcttt cctctacatt catctgttta tctgcttctc
    10861 tgatttcgtt tttcggatac aaatgcacat ctgtattgta tgtgtctttc catgtagaac
    10921 catcccacac gccatccacc atatcaggca tcatcactgg catcataaca accattggag
    10981 acgcgattct ggaaatatga gctggcgaat aagtttctaa gaaaagatag actttatctc
    11041 tagaagaagg tgcttcttcc gtcacttgga cggtagagaa taccgcttct ccagcttcat
    11101 tcgttgtttg tttgtcaata cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc
    11161 gtttttgaat ccattcaatc gcttcggcag aattaagacc gtcttctcgt tccggtgtta
    11221 aaggattatc tcgaatcaaa tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga
    11281 ctcctggaat attttcttca ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt
    11341 ccggaacaga tgagaaacct tttttgtgta atgtaatcgt cacttcactt ggtttttgtg
    11401 cgacttcttc ggctttggct agttttccgt tcccgaaaat ccctattccc gcagtaacga
    11461 gtaacagtac actcattccc gtgacaatca tttttttatt catttttttc tccccgttct
    11521 ttttttatt
//
LOCUS       DARBFD010000086        10801 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000086 DARBFD010000000
VERSION     DARBFD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.087"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            33..656
                     /locus_tag="SCK08_002218"
     CDS             33..656
                     /locus_tag="SCK08_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202070.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="SCK08_002219"
     CDS             1106..2155
                     /locus_tag="SCK08_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1202071.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="SCK08_002220"
     CDS             2152..3033
                     /locus_tag="SCK08_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1202072.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="SCK08_002221"
     CDS             3036..3806
                     /locus_tag="SCK08_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1202073.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="SCK08_002222"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="SCK08_002222"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEG1202074.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="SCK08_002223"
     CDS             5117..6280
                     /locus_tag="SCK08_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1202075.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="SCK08_002224"
     CDS             6397..6807
                     /locus_tag="SCK08_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1202076.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="SCK08_002225"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="SCK08_002225"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1202077.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="SCK08_002226"
     CDS             9380..9664
                     /locus_tag="SCK08_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202078.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="SCK08_002227"
     CDS             9824..10060
                     /locus_tag="SCK08_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1202079.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="SCK08_002228"
     CDS             10057..10455
                     /locus_tag="SCK08_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1202080.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3778 a         1792 c         2266 g         2965 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatgtata
    10681 ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc
    10741 gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt
    10801 a
//
LOCUS       DARBFD010000087        10737 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000087 DARBFD010000000
VERSION     DARBFD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.088"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..162)
                     /locus_tag="SCK08_002229"
     CDS             complement(25..162)
                     /locus_tag="SCK08_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1202081.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="SCK08_002230"
     CDS             complement(159..1343)
                     /locus_tag="SCK08_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEG1202082.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="SCK08_002231"
     CDS             complement(1599..1853)
                     /locus_tag="SCK08_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202083.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="SCK08_002232"
     CDS             complement(1878..3020)
                     /locus_tag="SCK08_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEG1202084.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="SCK08_002233"
     CDS             complement(3080..4432)
                     /locus_tag="SCK08_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1202085.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="SCK08_002234"
     CDS             complement(4503..4847)
                     /locus_tag="SCK08_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1202086.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="SCK08_002235"
     CDS             complement(4857..5231)
                     /locus_tag="SCK08_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1202087.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="SCK08_002236"
     CDS             complement(5244..5558)
                     /locus_tag="SCK08_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1202088.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SCK08_002237"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SCK08_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="SCK08_002238"
     CDS             complement(8976..9638)
                     /locus_tag="SCK08_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202089.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="SCK08_002239"
     CDS             10255..10485
                     /locus_tag="SCK08_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202090.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..>10737
                     /locus_tag="SCK08_002240"
     CDS             10491..>10737
                     /locus_tag="SCK08_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202091.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHR"
BASE COUNT         2962 a         2376 c         1824 g         3575 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggc
//
LOCUS       DARBFD010000088        10513 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000088 DARBFD010000000
VERSION     DARBFD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.090"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            14..1132
                     /locus_tag="SCK08_002241"
     CDS             14..1132
                     /locus_tag="SCK08_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1202092.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1260..2636
                     /locus_tag="SCK08_002242"
     CDS             1260..2636
                     /locus_tag="SCK08_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1202093.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3037..4617
                     /locus_tag="SCK08_002243"
     CDS             3037..4617
                     /locus_tag="SCK08_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEG1202094.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4815..5038
                     /locus_tag="SCK08_002244"
                     /pseudo
     CDS             4815..5038
                     /locus_tag="SCK08_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5243..5563)
                     /locus_tag="SCK08_002245"
     CDS             complement(5243..5563)
                     /locus_tag="SCK08_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEG1202095.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5711..7945)
                     /locus_tag="SCK08_002246"
     CDS             complement(5711..7945)
                     /locus_tag="SCK08_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1202096.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8424..8690
                     /locus_tag="SCK08_002247"
     CDS             8424..8690
                     /locus_tag="SCK08_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEG1202097.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            8690..10417
                     /gene="ptsP"
                     /locus_tag="SCK08_002248"
     CDS             8690..10417
                     /gene="ptsP"
                     /locus_tag="SCK08_002248"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEG1202098.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3344 a         1836 c         2151 g         3182 t
ORIGIN      
        1 aggaggaaat ctattgtttg aaaaacttag acagtctaaa ctaatgtttt ggtcgcttga
       61 attattgatc atagctgcat tgatcatgat ttcatcagaa ataaacttta ttttcgaacc
      121 gatcgggaca tttttttcca cgttatttgc acctgtactg attgcaggct ttctttacta
      181 tttattgaat cctattgtca agctgttgat gaaagcaaaa atcaaaagaa tatgggctgt
      241 cgcaattgtg ctgctccttt tagttgcagc aattatttgg attttactta gtgtcatccc
      301 aagtctagta cagcaaattt cctcattggc atctaatatg cctgatttca tcaaacaggt
      361 agaaagctgg ttaaaagagg tagccgaatt gcctttgttc aaggaagtag atatcaataa
      421 atattttgag caattagaca tttcttacgg gacaatcatt cagcaattcc ttagtggagt
      481 atccaatagt ttaggttcga tcgttggaac gatcgcttcg gcaaccgtag taatcattac
      541 agcaccattc atcttgttct atatgttgaa agacggagaa aaactagtgc caaacatcca
      601 acagtttttc ccagaaaaaa gaagaaacca aattgtggag ttattggggc aactgaataa
      661 aacattggct aattacatta gcggacaagc aatcgaatgt ttattcgttg gtaccttcac
      721 atttttagga tatatggtaa ttggtgtgga ttatgctttc ttatttgggg tcattgcggg
      781 attgacaaac ctaattccat atttaggacc ttatctagga ctagcacctg catttctagt
      841 aacagtcttc aatgatccgg taaaagcatt actttgttgt gtggttgtgc taatcgtcca
      901 acagttggat gggaatatca tttatcctaa tgtaattggg aaatcactga aaatccatcc
      961 attaacgatc attatcgttc ttctagtagc tggaaatatt gccggattgc ttggtatctt
     1021 cttaggtgtt cctttttacg caatctgcaa gacgatcatt tcctatataa tcaaaatcgt
     1081 caaagaagac aagcaaaatg aaaacaagaa gaaaattgct acgacctcgt aggtagtttt
     1141 agtacacttg tttttattgc aaagaaaatt gctttgtgat accattttaa cgtggctata
     1201 gtgccacgtt tttttctatc gtaaactaaa aattaatttt ataaggagag aatgcgatta
     1261 tgaatgctga ccctgagagt cagtcgctgt tagcgcagat tttattattg atcgttttga
     1321 cattgatcaa tgctttttta gcagcttccg aaattgctgt tgtttcgatc aataagaatc
     1381 gaatcgagca aaaagctgat gaaggagatg tgaaatctca aaagctttta aaaatattgc
     1441 agaatccaaa taatttccta tcgacaatcc aagtaggaat cactttggtc aacattttgt
     1501 ctggtgcttc actagctaat actttatcgg aacggttagc tccggtatta ggtggcggca
     1561 cagcggcaaa aaatattgca aatatcattg tgctggctat tttgacctat gtatcgattg
     1621 tttttggaga attgtaccct aaacgtattg ctttaaataa atcagaagaa gtcgcaaagt
     1681 tcacttcagg gctgatacgc ttgattggtg tagtagctaa gcccttcgtc tggctgcttt
     1741 ctgcttccac aagtttattg gcgcgtatca cgccgatgac ttttgatgat gaagattcaa
     1801 aaatgaccag agatgaaatg cgttatatgc ttgaaaacga aggcgtattg aataatgaag
     1861 aattagaaat gctccaaggg gttttttctc ttgatacgaa agttgcccga gaagtcatgg
     1921 tcccacgaac ggatgcattt atgattgata tcaacgacac tgtggaagaa aatgtgaatg
     1981 aagttttatc cgaaaactat tcaagaatcc ctgtttataa cgaagacaaa gataaagtag
     2041 tcggaatcct tcatacaaag aatttactaa aagctgccca taaattcggc tttgataatt
     2101 tagatatcaa gaaaatcatg caggagcctt tattcgttcc tgaaacggtc tttattgatg
     2161 atctgttata tgaaatgaaa aagacgcaga atcagatggc tattttatta gatgaatatg
     2221 gcggcgtcgt tgggttagca acactagaag accttttaga agaaatcgtc ggagaaatcg
     2281 atgatgaatc agatgaagtt gagaatcttt atgaaaaaat cgatgaacac gaatatatca
     2341 tccaaggacg tatgctgatt gatgagttca atgaagcttt tgacagtgat ctgcatatga
     2401 gtgatgtaga tacgatggct ggttatttga ttacagcatt aggcatgatt ccggatgaag
     2461 gcgaaaagtt atcgtttgat gtcgataata ttacgctggt ttctgaagaa atggaaggtt
     2521 ctcgaatcct taaaatccgt gtcatttttc atgatccgga agaaacagaa gcggaacctg
     2581 aagaagaacg aagatacttc agaaaagaat tcgaagatga cgagccaaga agataattgg
     2641 aaacttggtt atgaaagacg tgttgcttga ataaacaagc aacacgtctt ttcttttagt
     2701 cagctgacaa aaaaatattt gactcttatg gtttggtggc tttttcattc gctgaatcca
     2761 tgctatactg attcagttgt agtagttgac aaaggttgtg aaaaagctat cctgcattaa
     2821 gtaataagag cagccaagca aaaacagctt ttcgtatttt tggcttgggt gggcttattg
     2881 ctgataggct gtctctagaa cgccgtttac tcggaaaaag agactgctac acggcttttg
     2941 tcacagccct atatgaaatt gtctttcttt aagagatcgg acaatctgtg cctagagaaa
     3001 atataaataa ttgaatggaa gaaatggagt taggttatga ataatccaaa attaaagcaa
     3061 caagtggaca atcgcagaac ttttgcgatc atttcccatc cggatgctgg gaaaacaacg
     3121 attacagaac aattactatt gtttggtggg gctatccgtc aagccgggac cgtaaaaggc
     3181 aaaaaaacag gtaattttgc caaatcagac tggatggaaa tcgagaaaca acgtgggatt
     3241 tctgttacta gttctgttat gcagtttgat tatcaaggga aacgagtaaa tatcttggat
     3301 acaccaggac atgaagattt ttctgaagat acttatcgta ctttgatggc cgtggatagt
     3361 gcggtcatgg taatcgatag tgcaaaaggg atcgaagcac agacaaagaa attattccaa
     3421 gttgtaaaaa aacgtggcat cccgatattt acttttatca acaaactaga tcgtgatggt
     3481 cgtgaaccat tggaattgtt agaagaatta gaagagctat tagatatcga atcttatccg
     3541 atgaattggc caatcggtat gggtaaaggg ctagaaggtc tttatgatat ctaccataat
     3601 cgtgtagagt tttatcgtcc ggaaaattat caggatgaac gattagtaga attggatgaa
     3661 gatggactgc ttcccgaaaa tcatcctcta actgaaaatt ccttatatga acaagtctta
     3721 gaagaagtag aactaatcaa agaggctgga gatgcattta atcaagaaaa aattgctcgt
     3781 ggcgatcaga caccagtatt ttttggttct gcattgacta acttcggtgt acagactttt
     3841 ttagagactt tcgttgaatt ggcaccagct ccttatggac acaaaacaca agatgatcag
     3901 atggtaagtc cttatgagga agaattttca ggtttcgtat tcaagatcca agcaaatatg
     3961 aatccagccc atcgtgaccg aatcgctttt gtccgaatct gttctggaac attcgaacga
     4021 ggaatggatg tctggttgga acgtacaaat aaaaaactga aattaagcaa tgtcacacaa
     4081 tttatggcag attcgagaga aaacgtcgaa aaagcagtag caggcgatat catcggtgta
     4141 tatgatacag gcaattatca gattggggat acattgttcg aaggaaaact aaaagttgct
     4201 tatgaagaac tcccttcatt tactccagag ctgtttatga aagtgactgc aaaaaatgtc
     4261 atgaaacaaa aatccttcca caaaggaatc tatcaattag ttcaagaagg agcgattcag
     4321 ctttataaaa catatctaac ggaagaatat atcattggag ctgttggtca gctacaattc
     4381 gaagtattcc aatatcgtat gttaaatgag tataacgccg aagtaatcat gtctccaatg
     4441 ggacacaaaa tcgctcgctg gatcgatcca gaagatttgg atgagaagat gagttcaagt
     4501 cgcaatatct tggctcgtga ccgtttcgac caaccgttgt tcttattcga gaaccaattt
     4561 gcagaacgct ggtttgcgga taaatatccc aatgtaaaat taaaaagttt gatgtaaaga
     4621 agtcttgaca caagctgccc tgcatcaagt aataagaata gccaaacaaa aacagcctct
     4681 catattttgt ttagagccgt gacggatttt ccgtgacggc tctttttact atattaaaat
     4741 catagaaatg tctaatcaaa aaaatataaa aattattttg cgtacatatg attaaagaaa
     4801 ggagagtgtg acacatggaa acagaatggc aaagacgtga aagaaaaaaa cagaaaaaga
     4861 agagatgtta cgattatgtc gattattaga tgtaaaagac tgggacaaaa acgagcaaat
     4921 tgcacaaaaa gtacgatcaa tcatccataa agaggaaaaa gttaaaaata aagacacgcg
     4981 tagaactgaa aatgaaaaga gaaaagtaca aaagaaaaga gaaaaatcaa aaagctagaa
     5041 agaaaaagag gctgagacag aagcgtttaa ctccaagaaa taagctggaa ttcacaaaaa
     5101 tttgaaaagc aattttcgtg aatttcagct tatttccgaa ggagttgctt ctgctttcgc
     5161 cgtttatacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
     5221 aattattatg ttcaaaattt gtttacacgg ctaatgtagc gcctaattca tgttttggaa
     5281 tcgagatttc aacaaggcct gccaattcga ttactttgat ttcatcctcg tggtaaacgg
     5341 cgtctttgat tttatctaag aaataatttt tagcatcttc gatttctttc ggctggattt
     5401 tacgtttgtc attgatcagc agaatttcat tatgttcagg ggcttcagtg tagacaatag
     5461 tggtatttcc ggctgtgtag accttgacta gatgggcatc agtgttttca agctggttta
     5521 atacaagacg ggaatggctg tttgttacat ttaccaattt catggcgctc acctctttta
     5581 tgtattttta attgcttgtt tctcttatta taatttcttt tttataaaat gaacagtatt
     5641 ttgacttttc tgaaaaaaga atgagacggc atttctcaaa gaaataccgt ctcacgctat
     5701 aaaactggat ttattcagcc gtttcttcgg ctgattcttt agccaaacgt tctggtttag
     5761 aagtaacgat gatttttcca tctttatcca gttttgcttt caactcatgg atagaagggt
     5821 gatcaagata aaattcagcg ataccatctt cgatttgttc ttggatcgtg cgtcgaagag
     5881 gacgagcacc catagatgga tcatagccta agtcaactaa tttttctttg acattcgtag
     5941 gaacttcgat gtgaagttgc tgtgctgcca gcatttggtt gacatcgtct agcattagtg
     6001 tgacgattgt catcaagttt tcttttgata atgcactgaa ttcgatgatc ccgtcaaaac
     6061 ggttcagaaa ttctggtgta aagtagttgt ttaattggtt caatacagaa cgagtaacac
     6121 cttcgcgtgc cgcaccaaaa ccaacgtttg cttctacttt accagttccg gcattacttg
     6181 tcataatgat gatcgtatct ttaaagctga ccgttcttcc ttgtgcatct gtcaatcgac
     6241 catcatctaa gatttgcaag aacatatgca aaacatctgg atgagctttt tctacttcat
     6301 ctaacaagac aagactgtaa ggatttctcc gtactttttc tgttaattga ccagcttctt
     6361 cgtatccgac ataacctgga ggagaaccaa tcaatttaga gacactatgt ttttccatat
     6421 attcagacat atcgaaacga atcattgaat cttgtgaacc gaatagttcg taggcaagtt
     6481 gtttcgcaag ctctgtttta cctacaccag ttggtccaac aaataagaag gagccgattg
     6541 gacgattctg tttgtttaat ccaacacggt ttcggcgaat cgctttcgct actcgatcta
     6601 cagcgttatc ttgtccgatg acatgagctt tcaagtcatc tgctaagttt ttcagttgtg
     6661 tctgttcttt ttcttttaat tcacccactg gaatacctgt ccgttgttca acgatttttt
     6721 ccatatcttt ttcggtaatc acaggtgttt cctcttcagt gagctgacgt tctttcattt
     6781 tttgaagttt attgatctga tcgcgataat aagctgcttt ttcgaaatct tcttctcgag
     6841 aagctaaaac tttttgttct tctgcttctt gcagtttttt ctcgattgtt ttaggatcaa
     6901 cgatttggat cgtcaagttt ttcttagaac ctgtttcatc tagcaaatcg atggctttat
     6961 ccggcaagaa gcgatcttgg acgtaacgat tggataaagt tgcggctgct tcgatggcct
     7021 catcagtata ttttacatga tgatagtctt cataacgttt ttgcaaacct ttcaaaatac
     7081 tgatcgtttc ttcgacagtt ggttcatcta cccgtacagg ttgcatacga cgttctaaag
     7141 cagcgtcttt ttcgatgatg cggtactcat ttaaggtagt agcaccaacc atttgcaatt
     7201 ctcctcgagc taaagctggt tttaaaatat taccagcatc catatttcca tcaccagcag
     7261 aaccagcacc gacgatttcg tgaacttcat ctatgaatag gatcacattt tctgcttgac
     7321 ggatctcttc aatcaatttt tgcatacgtt cttcaaattg tcctcgtata ccagtacctt
     7381 ggactaaaga tacgacatct aaacgaatga cttctttgtc cattaatttt tgcggtacat
     7441 caccatctac gattttttgt gctaaacctt caacaactgc tgttttacca acacctggtt
     7501 ctccaatcaa gacaggattg ttcttggttc ggcgatttaa aatttcaatg actcgtttga
     7561 tttcgtcatc gcgtccgatc acaggatcga tatcaccttg tcgtgcttgt tctgtgatat
     7621 tgattccgta ttctcccaat aaaccttctg tattgccttg aggtggctga ccaccattga
     7681 agttgttgtt tccgccaaat tgtgttggtg gtgtttgctc ataatttcct tgctgttgca
     7741 tttggcgaga catggaacgg aaaagatcgt ccaaactgcc aaatccaaat ggatcatttt
     7801 gggccatatt agtcagacct ccattttgtt gatttttgat tttttgataa cagctctgac
     7861 agtaatcgag ttgtgtccgc tgaccattga cagtggcata caagtgaatc gttgcctcat
     7921 tcttgccgca gttttgacaa agcatgaatc attcacgtcc tttcacaaaa atgggtacat
     7981 ccatatttta catctcgttt ttcatgaggt aaaagaataa cccctagttt tttaagagat
     8041 ttatgaggta gaaaaggtga aatctctatt gaccattcct gacctttgac tcttattata
     8101 ctaaaaataa aaagggaagc aagtaattcg tttcatatga aaacaaagag ggtcaaagga
     8161 aagaatgata aacgtcagga gaagagaaag cgacaagaaa aaggaaataa acacgtttgt
     8221 tataaaatgt gaagaaaagg atgaaaaaga atccggaata atttgaatac ttttatctga
     8281 aattgaatga aaactgttgt atctaacgaa aaaaaatgtt aatctttaca gatgaaaggg
     8341 tggcatttgt taggattctc tgaattacta aaaaagaaaa ccctcacata aaaacaacac
     8401 tcacttaaag gagaccgatt aatatggaaa agaaagaatt tcacgtagta gcagaaactg
     8461 ggatccacgc tcgtccagct acactattag tacaaacagc aagcaaattt aactctgatg
     8521 taaacttaga gtataaaggt aaatcagtaa acttgaaatc aatcatgggc gttatgtctt
     8581 taggtgtagg tcaaggttct gatgttacta ttactgcaga aggtgctgac gaagctgatg
     8641 caatggcagc aatcgttgaa acaatgaaga aagaaggctt atctgaataa tggttgaaat
     8701 gctaaaaggg attgccgcta gtgacggtgt agccgttgca aaagcttacc tgctagttca
     8761 accggattta acattcagta aggcaacagt agaagatact gcggctgaag aagctcgttt
     8821 ggatgctgca ttagctaaat ctactgaaga attgcagcaa attcgtgaaa aagcagcgca
     8881 aagcttaggt gaagcagaag cgcaagtatt tgatgcccat ttgatggttc tttcagatcc
     8941 agaaatggta ggtcaaatca agcaaaatat taaagacaac agcgtaaatg ctgaatcagc
     9001 gctaaaagaa gtaacggata tgtatatcgg tatgttcgaa gcaatggaag ataatgctta
     9061 tatgcaagag cgtgctgcag atattcgtga cgttgctaaa cgtattttag cgcacttatt
     9121 aggcgttact ttaccaaatc catcaatgat caacgaagaa gtggtcgttg ttgcacatga
     9181 cttgactcca agtgatacag cacaattaga tcgcaatttt gtaaaagcgt ttgtaacaga
     9241 cattggtgga cgtacttcac attctgctat catggcacgt tctttagaaa ttccagcaat
     9301 cgttgggaca aaagaaatta ctgctaaagt caaagaaggc gatattttgg ctgtcaacgg
     9361 gatcgaaggc gatgtaatca tcgatcctac agatgaacaa aaagctgaat ttgaaaaagc
     9421 aggtgcagat tacgcagcac aaaaagcaga atgggaaaaa ttaaaaaatg cagaaactgt
     9481 cacagctgac ggcaagcatt ttgaattagc tgcaaacatc ggaacaccaa aagatctagt
     9541 tggtgtccat aataatggtg gagaagcagt tggtctgtac cgtactgaat tcctgtatat
     9601 ggattctcca gacttcccaa cagaagatga ccaatatgaa gcttacaaag ctgtattaga
     9661 aggtatggaa ggaaaacctg tagttgttcg tacaatggac atcggtggag ataaagaact
     9721 accatatctg caattacctc atgaaatgaa tccattctta ggatatcgtg cattgcgtat
     9781 cagtttatct gaacaaggcg acgaaatgtt ccgtacacaa atgcgtgcat tgcttcgtgc
     9841 atctgttcat ggtaatttac gtatcatgtt ccctatggta gctacactca aagaattccg
     9901 cgcagcaaaa gcgatctttg aagaagaaaa acaaaaattg ataagtgaag gcaaggaagt
     9961 atctgatacg atccaagtcg gcatcatgat cgagattcca gcagcagcag tcttagcaga
    10021 caagtttgca aaagaagtag atttcttctc tgtaggtacg aacgatttga ttcaatacac
    10081 aatggcagca gaccgtatga acgaacgcgt ttcttacttg taccaaccat ataacccatc
    10141 aattttacgt ttgatcaaaa atgtaattga tgcagcacac gctgaaggaa aatgggcagg
    10201 tatgtgtgga gaaatggctg gagatcaaac agctgttcca ttgctagtag gtatgggtct
    10261 agatgagttc tcaatgagtg cgacatctat tcttaaaacg cgtagtttaa tgaaacgctt
    10321 ggacacagcg aaaatggctg aacttgctga tcgtgcattg aaagaatgcg atatgatgga
    10381 agaagtcgtt gaacttgttc atgaatatgt aaaataaaca acgtatcaaa agctggtgtg
    10441 gcttgtaaaa gagctacgcc agtttttttg ttctttttta agaaaacgtt tttgtcagta
    10501 agaatagaca aaa
//
LOCUS       DARBFD010000089        10367 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000089 DARBFD010000000
VERSION     DARBFD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.091"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..305
                     /locus_tag="SCK08_002249"
     CDS             6..305
                     /locus_tag="SCK08_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1202099.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="SCK08_002250"
     CDS             378..1739
                     /locus_tag="SCK08_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEG1202100.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="SCK08_002251"
     CDS             1761..2021
                     /locus_tag="SCK08_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202101.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="SCK08_002252"
     CDS             2035..2787
                     /locus_tag="SCK08_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEG1202102.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="SCK08_002253"
     CDS             complement(2881..3486)
                     /locus_tag="SCK08_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1202103.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="SCK08_002254"
     CDS             complement(3539..4513)
                     /locus_tag="SCK08_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEG1202104.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="SCK08_002255"
     CDS             complement(4891..5610)
                     /locus_tag="SCK08_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1202105.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCK08_002256"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCK08_002256"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1202106.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="SCK08_002257"
     CDS             7161..8558
                     /locus_tag="SCK08_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1202107.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="SCK08_002258"
     CDS             8639..9079
                     /locus_tag="SCK08_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1202108.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="SCK08_002259"
     CDS             9227..9553
                     /locus_tag="SCK08_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1202109.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="SCK08_002260"
     CDS             9554..10303
                     /locus_tag="SCK08_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202110.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3288 a         1828 c         2149 g         3102 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgctt
//
LOCUS       DARBFD010000090        10194 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000090 DARBFD010000000
VERSION     DARBFD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.092"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            121..627
                     /locus_tag="SCK08_002261"
     CDS             121..627
                     /locus_tag="SCK08_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEG1202111.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            644..1543
                     /locus_tag="SCK08_002262"
     CDS             644..1543
                     /locus_tag="SCK08_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEG1202112.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1630..2514
                     /locus_tag="SCK08_002263"
     CDS             1630..2514
                     /locus_tag="SCK08_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1202113.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2706..2777)
                     /locus_tag="SCK08_002264"
     tRNA            complement(2706..2777)
                     /locus_tag="SCK08_002264"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2742..2744),aa:Arg,seq:ccg)
     gene            2837..4171
                     /gene="rpoN"
                     /locus_tag="SCK08_002265"
     CDS             2837..4171
                     /gene="rpoN"
                     /locus_tag="SCK08_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEG1202114.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4320..5411
                     /locus_tag="SCK08_002266"
     CDS             4320..5411
                     /locus_tag="SCK08_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEG1202115.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5452..6453
                     /gene="gap"
                     /locus_tag="SCK08_002267"
     CDS             5452..6453
                     /gene="gap"
                     /locus_tag="SCK08_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1202116.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6563..7753
                     /locus_tag="SCK08_002268"
     CDS             6563..7753
                     /locus_tag="SCK08_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEG1202117.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7857..8612
                     /locus_tag="SCK08_002269"
     CDS             7857..8612
                     /locus_tag="SCK08_002269"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEG1202118.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8728..10026
                     /gene="eno"
                     /locus_tag="SCK08_002270"
     CDS             8728..10026
                     /gene="eno"
                     /locus_tag="SCK08_002270"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEG1202119.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2631 t
ORIGIN      
        1 tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa
       61 aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga
      121 atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat
      181 ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat
      241 aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa
      301 aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca
      361 ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc
      421 ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata
      481 gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa
      541 attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg
      601 aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga
      661 aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga
      721 ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa
      781 gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct
      841 tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat
      901 tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc
      961 gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata
     1021 ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac
     1081 ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt
     1141 gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac
     1201 aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta
     1261 tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc
     1321 atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact
     1381 tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc
     1441 tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt
     1501 gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga
     1561 ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga
     1621 agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg
     1681 tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct
     1741 tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca
     1801 actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag
     1861 tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc
     1921 gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta
     1981 aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct
     2041 gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat
     2101 cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact
     2161 gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc
     2221 acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa
     2281 gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat
     2341 ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg
     2401 atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat
     2461 ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga
     2521 caagtatctt taataaataa cacattttaa taagtagttg aacacataac taccttactt
     2581 gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg
     2641 cggacagttt tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag
     2701 tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc
     2761 cactacacta aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag
     2821 gggattggac aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca
     2881 gaaactagcc atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga
     2941 ggagctgttg gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga
     3001 gccggaatgg cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac
     3061 aaattaccta aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca
     3121 agtccatttg aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata
     3181 catcgatttg aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc
     3241 tacgcctatc caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt
     3301 aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc
     3361 ggaattagct tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa
     3421 agaaatcgca caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt
     3481 acaaacgttg agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat
     3541 tcctgatgtc cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa
     3601 aaatcagcca aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga
     3661 taaagaaact gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac
     3721 gatcctacaa cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa
     3781 agctttcttt atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac
     3841 tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac
     3901 agacttcggt gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac
     3961 agaacagaca gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga
     4021 tcaagaagac aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga
     4081 tgagatagcg atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag
     4141 ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca
     4201 ctttttctag tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag
     4261 gttgagattt ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata
     4321 tgggtcgttt taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg
     4381 atgaaataaa aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct
     4441 ataaaatttt acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa
     4501 gtatggggat cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt
     4561 tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa
     4621 gccttgaaaa cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag
     4681 aatattttgg taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag
     4741 tggctgatgc attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg
     4801 agaatatcat cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta
     4861 atctggaaaa taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag
     4921 cgataactgt acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc
     4981 accgtgcact ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg
     5041 agccatctat ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta
     5101 ttgggcgtgc attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc
     5161 tgaaacaagc aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag
     5221 tcgtttataa ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca
     5281 tcatggcaat cgcgggcgga aaatcaaaag ccaaagcaat tcgagcttat atgaaaaatg
     5341 caccaaaaca aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg
     5401 taacccttta aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt
     5461 aaagtaggta ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat
     5521 gtagatggaa tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac
     5581 ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt
     5641 tcattcaacg taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta
     5701 ccatggggcg aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa
     5761 tcagcagctg aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc
     5821 ggaaacgatg ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa
     5881 acagttatct caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg
     5941 aacgacaaat ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac
     6001 caaatgactc tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct
     6061 gcaaacatcg ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa
     6121 ttgaacggta aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca
     6181 gaattagtaa cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa
     6241 gaagcttcaa acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt
     6301 ggtatgactt acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac
     6361 aaacaattag ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta
     6421 gtacgtactt tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta
     6481 tagacatgta catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata
     6541 agtttattca ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga
     6601 caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga
     6661 cgacactcgt atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa
     6721 agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc
     6781 tttagctcca gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc
     6841 tgaaactcgc ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt
     6901 cgtattcgaa aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac
     6961 agaactaggt aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac
     7021 agctcaccgt gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc
     7081 tggattcttg atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg
     7141 accaatgatt gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa
     7201 cttgattcct aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga
     7261 agcaaaagga atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa
     7321 agaattaatc gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc
     7381 agaattttca aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat
     7441 ggctttagac atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa
     7501 aacagttgtt tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac
     7561 aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg
     7621 agactcagcc gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac
     7681 aggtggcggc gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat
     7741 caacgataaa taaacgacca agtttgctca attctgacga gtaatcgatc aaaaggtgaa
     7801 gggagttttc atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc
     7861 gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat
     7921 ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat
     7981 ctcctgcatt attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct
     8041 cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag
     8101 ctcttgctga tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt
     8161 tccatgaaac agacgaagac atcaataaaa aagcgaaagc tatctttgca aatggtatga
     8221 caccaatcct atgctgtggc gaatctttag aaacctatga agcaggcaaa actgctgaat
     8281 ggatcgaagg acaaattaca gctggtttag ctgatctttc agctgaacaa gtgtcaaaca
     8341 tggttattgc ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa
     8401 tcgctgacga aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag
     8461 tatcagaagc agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat
     8521 acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt
     8581 cattcttgtc attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga
     8641 aatgtttgca ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa
     8701 aaaacaaact caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag
     8761 atcttagact cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct
     8821 tttggccgcg gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta
     8881 cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta
     8941 aacaacatca ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac
     9001 aaagctatga tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt
     9061 ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac
     9121 cactacctag gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac
     9181 ggcggatcac atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc
     9241 gctccaacat tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca
     9301 atcttgaaag ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac
     9361 cttggttcaa acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat
     9421 gtacctggta aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa
     9481 gaaaaaggtg tttacgtact agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg
     9541 atcaaattct acgaagaatt agtttctaaa tatccaatca tctcaatcga agacggatta
     9601 gacgaaaacg actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa
     9661 ttagttggtg acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa
     9721 ggaattgcta actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc
     9781 gaagctatcg aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt
     9841 gaaacagaag attcaacaat ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa
     9901 actggttctc tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa
     9961 gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac
    10021 aaataattct tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc
    10081 acttagttaa gtaatcgaac tctctacttg ctgaaatatc ggctctttgt caataaggac
    10141 tgatgagttg tgcaaaatca aatctgagtc agaatgaacc acattctggc tcag
//
LOCUS       DARBFD010000091        10191 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000091 DARBFD010000000
VERSION     DARBFD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.093"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            214..498
                     /locus_tag="SCK08_002271"
     CDS             214..498
                     /locus_tag="SCK08_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEG1202120.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="SCK08_002272"
     CDS             complement(779..1198)
                     /locus_tag="SCK08_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEG1202121.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(1200..1784)
                     /locus_tag="SCK08_002273"
     CDS             complement(1200..1784)
                     /locus_tag="SCK08_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEG1202122.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="SCK08_002274"
     CDS             complement(1831..3306)
                     /locus_tag="SCK08_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEG1202123.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="SCK08_002275"
     CDS             3487..3798
                     /locus_tag="SCK08_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202124.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="SCK08_002276"
     CDS             complement(3913..6249)
                     /locus_tag="SCK08_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1202125.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="SCK08_002277"
     CDS             6391..7059
                     /locus_tag="SCK08_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1202126.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="SCK08_002278"
     CDS             7062..8105
                     /locus_tag="SCK08_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1202127.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="SCK08_002279"
     CDS             complement(8342..9223)
                     /locus_tag="SCK08_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1202128.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="SCK08_002280"
     CDS             9382..10110
                     /locus_tag="SCK08_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEG1202129.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
BASE COUNT         3021 a         1848 c         2060 g         3262 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cgacaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tatgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatgtttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattc caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcactcatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgaa ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg atccgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atctgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gtttttttgc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattggac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaattt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 cttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tctgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg a
//
LOCUS       DARBFD010000092        10086 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000092 DARBFD010000000
VERSION     DARBFD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.094"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(247..759)
                     /locus_tag="SCK08_002281"
     CDS             complement(247..759)
                     /locus_tag="SCK08_002281"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEG1202130.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SCK08_002282"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SCK08_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEG1202131.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="SCK08_002283"
     CDS             complement(3212..3655)
                     /locus_tag="SCK08_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEG1202132.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="SCK08_002284"
     CDS             complement(3656..4444)
                     /locus_tag="SCK08_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1202133.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="SCK08_002285"
     CDS             complement(4441..5382)
                     /locus_tag="SCK08_002285"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEG1202134.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="SCK08_002286"
     CDS             complement(5559..6146)
                     /locus_tag="SCK08_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEG1202135.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SCK08_002287"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SCK08_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEG1202136.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="SCK08_002288"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="SCK08_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1202137.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="SCK08_002289"
     CDS             complement(9063..9998)
                     /locus_tag="SCK08_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1202138.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DARBFD010000093        10084 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000093 DARBFD010000000
VERSION     DARBFD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.095"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            162..392
                     /locus_tag="SCK08_002290"
     CDS             162..392
                     /locus_tag="SCK08_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEG1202139.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="SCK08_002291"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="SCK08_002291"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1202140.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="SCK08_002292"
     CDS             1803..1979
                     /locus_tag="SCK08_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1202141.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="SCK08_002293"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="SCK08_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEG1202142.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="SCK08_002294"
     CDS             2474..2941
                     /locus_tag="SCK08_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1202143.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="SCK08_002295"
     CDS             3026..3910
                     /locus_tag="SCK08_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1202144.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="SCK08_002296"
     CDS             4024..4260
                     /locus_tag="SCK08_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEG1202145.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="SCK08_002297"
     tRNA            4348..4428
                     /locus_tag="SCK08_002297"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="SCK08_002298"
     tRNA            4435..4506
                     /locus_tag="SCK08_002298"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="SCK08_002299"
     CDS             4631..4801
                     /locus_tag="SCK08_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202146.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="SCK08_002300"
     CDS             5021..5728
                     /locus_tag="SCK08_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1202147.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="SCK08_002301"
     CDS             complement(5788..6432)
                     /locus_tag="SCK08_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEG1202148.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="SCK08_002302"
     CDS             6559..6846
                     /locus_tag="SCK08_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202149.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="SCK08_002303"
     CDS             6954..7274
                     /locus_tag="SCK08_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1202150.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="SCK08_002304"
     CDS             7355..8668
                     /locus_tag="SCK08_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1202151.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="SCK08_002305"
     CDS             8665..9384
                     /locus_tag="SCK08_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEG1202152.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="SCK08_002306"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="SCK08_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEG1202153.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DARBFD010000094        10041 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000094 DARBFD010000000
VERSION     DARBFD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.096"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            27..536
                     /locus_tag="SCK08_002307"
     CDS             27..536
                     /locus_tag="SCK08_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1202154.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            629..1791
                     /locus_tag="SCK08_002308"
     CDS             join(629..905,905..1791)
                     /locus_tag="SCK08_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1202155.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2071..3051
                     /locus_tag="SCK08_002309"
     CDS             2071..3051
                     /locus_tag="SCK08_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1202156.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3173..4591
                     /locus_tag="SCK08_002310"
     CDS             3173..4591
                     /locus_tag="SCK08_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEG1202157.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4662..6131
                     /locus_tag="SCK08_002311"
     CDS             4662..6131
                     /locus_tag="SCK08_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1202158.1"
                     /translation="MDYKKNAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6141..6608
                     /locus_tag="SCK08_002312"
     CDS             6141..6608
                     /locus_tag="SCK08_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1202159.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6644..7516
                     /locus_tag="SCK08_002313"
     CDS             6644..7516
                     /locus_tag="SCK08_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1202160.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7597..>10041
                     /locus_tag="SCK08_002314"
     CDS             7597..>10041
                     /locus_tag="SCK08_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1202161.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDF"
BASE COUNT         3495 a         1451 c         2008 g         3087 t
ORIGIN      
        1 gttttattgt ttagggctat actatattgg tacaaggaat agaaagaggt gtttatgtgg
       61 ttgataaaat tcagtttaat caagttatag aagaattcat caaagtaaat aatttaatga
      121 cccatattca aaaacgacca atcagaataa ctaatgatct aaaactcagt acgagtatga
      181 ttcatttgat tgatgtgatt ggaaattatc ctgaaactag tattacggaa ttagctgatc
      241 gattaggcgt taccaaagga gctgtatccc aacagattcc cactttagag aaaatgaatc
      301 ttataagcat ttctcaaaaa aaaggaaata agaaaactaa acttgtatcg ttaacaaaat
      361 atggtcgaaa ggtgtacgaa tcacataatt atttacatga agagctttat agctcaataa
      421 atgaagcgtt agaatcattc tcaccagacc aaatttcaac cattaaacag attctagaaa
      481 aagtatctta tacaatcaat gaatatcaag taagtctgac aagaaaggaa gattgacatg
      541 aaaaaaatga tttttgaatc ggctacacta gactagacag aaaaaataag gtgtgtaaaa
      601 taaatgaaaa cacactggag gaatttttat gtcaagacgt caacgtagaa cctattcaaa
      661 agaattcaaa caacaagtcg tcgatcttta tctcgcagga aagccccgag ctgaaatcat
      721 tcgagattat gagctgacta cctcggcctt tgataagtgg gtcaagcaag cgcaaactac
      781 tggctcgttt caggaaaaag ataacttgat ccctgaacaa gtagaactaa ttgcattacg
      841 aaagaaaaat aaacagcttg aaatggagaa tgacatttta aagcaagcgg cgctgatatt
      901 gggacgaaaa gacaagtaat tgataccaat aagcacaaat actcaatatc agcgatgtgt
      961 aaagtgttaa acatctcttg ccaaacctat tattatcaag ccaaaccagt tgaagatgaa
     1021 tcagaactgg aagaactcgt tcaagaagaa tttattcgca atcgaaaagc ttatggtacc
     1081 aggaagctga agcagtgctt ggccaatcta ggtcttcaag tgagtcaacg cagaatcggc
     1141 cgtatgatga aacagcgtgg cttgcgttca acatacacat tggctcattt caaattccat
     1201 tcttctactt gtaacgaagc caagaccgcc aatattctag acggaacctt cctacaggaa
     1261 aagtcattag aagcgattgt aaccgacctt acctatgttc gtgttgggaa gaaatggcag
     1321 tatatctgcc tgattcttga tttgtttaat cgagaaatca tcggctattc atgcggggag
     1381 aagaaagatg cgacgttagt caaggaagct tttgcaaaga ttccgtactc gttgaataat
     1441 gtacaacttt ttcacacgga tcgtggaaaa gaattcgata accagacgat tgacgaaatt
     1501 ttatgggcct ttaatatcac gcgttcctta agcaaaaaag gctgccccta tgataacgcc
     1561 gttgcagaat ccacctataa atctgtcaaa gtagaattcg ttcatcaata ccgctttgag
     1621 acattggcta atttgcgatt agagctattt gattacgtcc attggtggaa taccatccgg
     1681 ctgcacggca cgttaggcta tgagacaccg atcagttttc gacagcagag attggcgcag
     1741 cgaatccttg ataatgagct cggatgtgat accggtggcg aggcacctta attatttgaa
     1801 tgagcttctg ccttagagaa tcagattcga ggaggctcgt tgtcaagggc agtcggaaca
     1861 ccagcagcaa gcttacgcaa taccttataa tttttgtcaa aaaaagtgtt gccattccac
     1921 atatatcttt aaacagctat tgccagataa aaataaatga tattatatat ataaatactt
     1981 atccgaaaag gggaatgaga gagtcaaggt tcttggaatg tcgaatagtc gagtaggtag
     2041 attttctatc tacaaccttg ttgtttaaaa atgaaaaaaa ttggaattag agaagttgcg
     2101 aaagaggcaa atgtttctcc tacgactgtg tcgagggtat tgaatgatcg tggatatttg
     2161 agtgaggaaa caaaaagaaa agtttttgat gccatgaaaa aattagaata ctatcctaat
     2221 gaactggcga gagcactttt taaaaacgaa acatataccg taggtttgat ttttcctaca
     2281 attactaatc catttcacgc tgagttaatt caagatatcg agttcgagct ttccagcagg
     2341 ggatataaag tgttgctgtg caatagctta aacaatccaa caaaagaaaa agagtacttg
     2401 acgatgttga gaaagaatca ggttgatgga attatagtag gaacgcacaa taaagatatt
     2461 gcggattatg atattccagg gctacctatt gtagcgattg atagaagtct tggtgaaaat
     2521 acagttacag tatcttgtga taattacagg ggtggggaat tagctactca actacttgta
     2581 aatcatggct gtcagaatat actgtgtatt cggggagata gtaagataaa gctacccgcg
     2641 aatagtagga ctttagcata taaagatgtt ttgaataaat tcatgctgga accgttagtc
     2701 ctagaagtac catttgtaaa agaaatcaat gaaaaaaatg aaataattaa gacatatctg
     2761 gaaactcatc caaatattga cggggttttt gcaggagatg atttattggc ttctttggcg
     2821 attaacaact taggaagttc gggcaaaaga gttccaaaag aggttaaagt gattggattt
     2881 gatggagctc aacaaacatt gatttataat ccagaattat caactattca acagcctata
     2941 gatcaaatat ctaaagtagc agtaaaaaaa ttattgaata tgattaaggg cgaaaaggag
     3001 acggatgaac tttcacttcc agtaaagctc ataaaaaaaa atacagcata agttaaatcg
     3061 gcgaaaaatt ccttcgtcga tttaaaaata tttttatgaa acgggttgac atatgaaacg
     3121 ggttgacata tattagttgt gaagataaaa accttttcac tggaggaaga aaatggagaa
     3181 attattaatt aaaagcaata atcatcaact ttactaccaa cctaaggatg tttgggtcgg
     3241 agatgttatg ccttacggga aagaaggaaa attctttatt tatcatcaac gtgatactag
     3301 aaatccagtt ccttttggag aaccgtttgg ctgggcttta gcaactacga aagattttgt
     3361 tcattttgaa gactttgggg aatctctttt aagaggggat gacaatgaga gagaccagtt
     3421 tatctacgct ggttctgtat ttgaggcaaa tggaaaggtt cacgcatttt atacagggta
     3481 taatcgtgaa ttcttagcgc agggaaagac atctcaagtg ttgcttcatg ctgagagtga
     3541 tgattttgtt cattgggaaa aatcagaaac agcattggag ttagaaccac aagaagggta
     3601 tgatattcga aactggagag atccatttgt cgtttgggat gatgaaaaag aagaatattt
     3661 gctaatttta ggggcgcgaa agggtcagga taaacatcta caaaccggac gtttagtgga
     3721 atttacatca aaagatttaa ctaattggga attcaaaggt gatttttggg ctccgggatt
     3781 gtataccatg tttgaaatgc cagaactttt caagattggt gactggtggt atcttgtcta
     3841 ttcagaatac tcagatggaa ataaaatact ctatcgaatg agtcaaagtt tggaaggtcc
     3901 ttggattaag cctaaagatg aagcttttga tggacgggct tattatgcag gtagaacagc
     3961 ttatgatgga aaacgtcgag tcctatttgg atgggtacca acaaaagatc aagaaaatga
     4021 taaaaacaat tatttatggg gaggtacttt ggttcctcat gaagtctatc aaaaagagga
     4081 tttttcactt ggggtaaaac ccatagacga agtgtggaat gcattcataa atttacatga
     4141 tattgccgat gtagagatag aaacagcaga cacaagagaa gaagtaacgc ttgtcaaaaa
     4201 tagtggaact ttgtttaatt tagaaacaac aatttcattt gatgagaatc ttagtgactt
     4261 ttcactgaga ttatataaaa accttgagac agatgaatct tacgaatatc gcttcaattt
     4321 agatgagggg cgtcttacat ttgataaaaa cccttgttac ccttggtttc aatatatgga
     4381 taaaggactt attagaccta tatctttaaa gcctaaccaa tcttataaac taaaagtgat
     4441 tgttgatgac aatataatga cactttatat tgatggaatc gctcttaacg ctcgtgtaca
     4501 tggttgtttt ggtcaagcaa ttactattac agcaacaaat ggatcaatga ctgcaataaa
     4561 taccaagttt tcaaatgaaa taaagaaata ataaacgtca atcatgtggc aggtctgaaa
     4621 atcaaacatg acctgtcaca tttaaaaggg aggataaatg aatggactac aaaaaaaatg
     4681 ccgaagatgt attggaaaac gttggaggaa aagagaatat ctcaagcgct acacactgca
     4741 ttactcgttt acgtttaatt cttaaagaca aaagcaaagc gaatatggaa atgcttgagg
     4801 aaatagaagg tgcaaaaggt gttcttttca atagtggaca gttacaagta attttcggaa
     4861 caggtataat tgaaaaggtt tacaaagagt tcgttgaatt gacaggagct aaggaagtat
     4921 ccgtttctga aatgaaagac gaaggagtca ataattcagg aaatagactt caacgagctt
     4981 ttaaagtttt ctcggatatc tttattccga tcattccagc ttttattggg gcagcaatgg
     5041 ttttaggaat tcgttcgttg attaccactc ctggaatgtt tggaatggat ggctcattcg
     5101 ctgatcacaa tgtattccta aatgatttat ccaaatttct gggtattatt gcaaccacat
     5161 ttgattattt accagtttta gttatgtatt ctgcaacaaa acgttttggc ggaaacccta
     5221 ttttaggatt acttgtagga tttgttatga ttcatccagg actagccaat cggaatgatt
     5281 tcgtcatggg aactataaag cctgattatt ggcatttatt tggattaagc attccagcag
     5341 ttgcgtttca agggggtgtt tttccagcaa ttttgacttc ttggttttta gcaaaagttg
     5401 agaaaattac aaataaatat tctcctcaag tacttgctta cattttagtc ccaactgtga
     5461 caattgttct tgccaacttt gcactatttt tagtgtttgg tccattgggg aattttatcg
     5521 gtaatgtttt aggtggaatc attgatttcc tatatatgaa gatgggtgcc tttggtgctt
     5581 ttgtttttgc aggcgcgtta caaccattag ttgtaacagg aacacaacat gctattcaag
     5641 gtattgaggc aaacttagtc gcaacaactg gattcaatta tatccaacct atttggtcag
     5701 tttcaattat tgcccaaggt ggcggttgta ttggtatgta tttactcttt aagaaaaaat
     5761 ctaaagatag agatattgcg atttcaagct ttattccaac actggtaggt attactgaac
     5821 cggctatctt tgctgttaat ttgaaatatt caatcgttcc ttttgtttgc tcgtttattg
     5881 gtgctggttt tggtggagca tttatgaaaa tttttgatgt aaaagccatt ggacaaggat
     5941 tgactgtatt gccaggtctg actattgcta atccagttct tccttatata tttggtaatc
     6001 tcatagcatt cattttacca atcattttta ttctcgctta tgataaatat aaagtgttta
     6061 tccctgaagc taaacaccgt aaatttgcag aaaaaactga ctcaaaaaaa ttgccagttc
     6121 aagtaaagtg agatgagagc atgttaaatt tttttagaaa aaatgaaata agcgttgtag
     6181 cacctgcaac cggtgttttt gaaaaactgg agaaattaag tgatccagtc ttttcaaaag
     6241 gaatgatggg acaaggattt gcaattgatg caaaaaacga aacgattgta tctccaatcg
     6301 atggttttgt ttcaagtatt tttcctacaa aacatgcctt aggaataaaa acaaaatcag
     6361 gaattgagat acttgttcat gtaggaatag atacagttga gctaaatggt gagggatttg
     6421 atattaaagt tcaagaagga caaaaagtta aggctgggga tattcttatg accattaatt
     6481 ttgtagtttt tgaaaagaat cacaagacta aagatgtcat tgttgttttt ccagactacc
     6541 aagacgaaat tttcattccg aaacttgaag aagtaaaaca aggacaagaa ttgtttgttc
     6601 ttggctaatg ataagtaatt aataatagaa tcgaggaaaa tgtatggttt ttggggctat
     6661 tgaagctggt ggaacaaaat ttgtttgtgc cattggagat aaagatttga acatatttga
     6721 aaaagtcagc tttcccacga ctacacccaa agaaacaatg aatttagttt tagatttttt
     6781 taatcatcat aaagatgatt tagaaggtat cggagtggga tcgttcggtc ctattgatat
     6841 tcattgtgaa tctaaaacgt atggcttcat tacatcaacg ccaaaacttg cttggcagga
     6901 ttttgatttt attggaagtt taaaaaagca acttaatatt ccaactgctt ggacgactga
     6961 tgtaaatgcc tcttgctatg gtgaatatgt ttctggaaat ggcagaggcc tttcaagtgt
     7021 tgtttactat accgtaggaa cgggcatagg aggtggcgct atccaaaacg gaaacttcat
     7081 tgaaggtttt agtcatccag aaatgggaca tatgcttgta aaacgacaca ataaagacaa
     7141 cttttctggg acatgtccat tccacggaga ctgtttagag ggcttagctg ctggtccggc
     7201 aatcgaaaga agatcaggga aaaagggaca agaagtgact gaaaatgacg ttacttggga
     7261 aatagaagct tattacattg ctcagtgtgc atataataca acattgatgt tatcacctga
     7321 tgtcattata tttggtggcg gagtaatgaa acaaaaacac ttaaaaaaga aagtacaaga
     7381 taagtttatt gagttgatca acggatatgt cgctattccc gatgttgaca attatattat
     7441 gacaccaaaa ttgttagata attctggtat tgtaggttgt ttagctttag caagagacgt
     7501 taaagaaaaa tcttaatttg gaaaaaagat cgtctcgact atttgtttgg ttgatgcgat
     7561 ctttttttca attagtaatt gaagggacgc ctaaaagtga aaaaaaaaat gaaagatgaa
     7621 gtattagagt atatcaaaaa taatttaaag tattacgatt tttctgctca agatattgca
     7681 atgaaatttt gtattaaaag aaatgtcgct tcccactatt tgaatcagtt attttcagat
     7741 ggaaaattac taaagaatga ttctgttaga ccagtaatgt tcaagtataa tcaacaaaag
     7801 ccaaaagact gcttttctaa gtttattgga gcagacatat ctttgaaatc cactatagat
     7861 aaatgcaaag ctactgtaat gtatccccct aatggcctcc cattaataat aaaaggaaac
     7921 agtggcgttg gaaaaagttt tttagcaagt ttgatctatc agtatgctct tgatagaaaa
     7981 gtaattcata atgacgcaaa atttgttgtt gtgaattgtg cagattatgc aaataatcca
     8041 gagctattgt cagcagtttt atttggatat aaaaaaggag cctttactgg ggctgaaaaa
     8101 gatactgcag ggatgctatc ttctgctaat ggaggatacc tttttttaga tgaggttcac
     8161 aatctaagtg cagaaaacca agagaaattg tttcttctta tggattctca aaagtatcgt
     8221 aaacttggtg aaagtgtgca atgggagtat gcaaatgttc gccttatctt agccacaaca
     8281 gaagataaaa atacctcttt gttagcaact tttagaagac gcatcccagc tgaaatcact
     8341 ctgccagatt acaataatag aagtacatct gaaaaaattc aattattatt tgaatttctt
     8401 aaagatgaag caattacgat tgattcaaaa atattttgtt cgattgaact ttttactgat
     8461 cttttatcga aaacatttga aggaaatgtt ggagaattga ggaatgaaat caagcttctt
     8521 tgtgcagaag gttatttagg aaataagaga aagtcttcaa tttatttagg ggacaaacta
     8581 gacagcggtt tttggataga tccagaaatt agtatagatt taaaaaaaat gtttcttaaa
     8641 tcactttctg aaattgattt gattgaactt tttcaaaatc acatttcaat tgaagaaagc
     8701 atgagtcaat taagagatag aatacttaaa gagtgtgcag gttcttctga gtataattat
     8761 ttagaaagtg acgaatttat agcattaaga aactatgttg ttaataaaat atcaccaatt
     8821 attgattcga caggtctatg tttattagat gaattcttgg cggatatcag tctatttata
     8881 atgtttattg atgtaattga agaaacatca agaaattttt tgctgtctaa gttcagaaaa
     8941 tttcgtatga aaaatgataa acaaaaatta ttggcaaatg agatttggag tagtttagaa
     9001 gtcgaagaaa gtaaacaaga acttttgttg aaatggatac tttttcttgt aaagaagttc
     9061 tatgtgaaaa taccagaaac ccattgtctg atagttatgc acggtaaaat aacagcttct
     9121 gcgatcgcaa gggaaacaaa taagttgctg aatacatatg tttatgaggc tttcgatatg
     9181 ccaattgaag gtgagactag tgacttaatt aacctaatca acaatttttg taaaagtata
     9241 gatacgacga atggactcat tctgctagtt gatatgggat cattggaaca aatgtatgaa
     9301 aaaattgaat ccaatgtaat aggtgatttg gtaattttaa acaatgtttc tacagcttta
     9361 gcgattgaat gtgggattca agtttgccaa aaaaagccaa ttagtcattt ttaccagatg
     9421 gattttgatt cgtttcaagt taaagttcag tattataaag gattatcaca aaagaaaaat
     9481 gtaatagtat cttgtctctc tggtgagggg atttctgaaa aagttaagga tattttaaaa
     9541 cggtatctcg ataatcaagt ggagattttg atttttgatt ttaacgcatt aaagaaaata
     9601 gcaaaggaaa aagatactat gatttttaag aatacaattt gtgttctttc aaccactgaa
     9661 ttttacattc aaggaatcga ttgtttaaat ttagaaaatc taattaatgg gaatcaaacc
     9721 ttagaaaaat taaataagta tatggactcg gatagctgtg agttctgctt aaatgaatta
     9781 gttaaactct ttactattga aggcgctagt actaagcttc gctttttaga ttccaaaaaa
     9841 gtattcaatg agatagaaaa agtactatat ttgtatgaaa aatattacga tgtcaaaatc
     9901 ccaagttttc tgagaattaa tctatttcta catctttctg gaatgattga acgaattatg
     9961 attggtgatg gaataacaaa tgaacatttg aaagagggag cgcaactatt tgatactttt
    10021 ttaaatgtat caaaagattt t
//
LOCUS       DARBFD010000095         9886 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000095 DARBFD010000000
VERSION     DARBFD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.097"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            69..3296
                     /gene="fss3"
                     /locus_tag="SCK08_002315"
     CDS             69..3296
                     /gene="fss3"
                     /locus_tag="SCK08_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEG1202162.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3409..3723
                     /locus_tag="SCK08_002316"
     CDS             3409..3723
                     /locus_tag="SCK08_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1202163.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3736..4110
                     /locus_tag="SCK08_002317"
     CDS             3736..4110
                     /locus_tag="SCK08_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1202164.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4120..4455
                     /locus_tag="SCK08_002318"
     CDS             4120..4455
                     /locus_tag="SCK08_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEG1202165.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4538..5878
                     /locus_tag="SCK08_002319"
     CDS             4538..5878
                     /locus_tag="SCK08_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1202166.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5956..6630
                     /locus_tag="SCK08_002320"
     CDS             5956..6630
                     /locus_tag="SCK08_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEG1202167.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6623..6787
                     /locus_tag="SCK08_002321"
     CDS             6623..6787
                     /locus_tag="SCK08_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202168.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6863..8005
                     /gene="dcm"
                     /locus_tag="SCK08_002322"
                     /pseudo
     CDS             6863..8005
                     /gene="dcm"
                     /locus_tag="SCK08_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            7995..8285
                     /locus_tag="SCK08_002323"
     CDS             7995..8285
                     /locus_tag="SCK08_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202169.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8544..9728
                     /locus_tag="SCK08_002324"
     CDS             8544..9728
                     /locus_tag="SCK08_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1202170.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9725..9862
                     /locus_tag="SCK08_002325"
     CDS             9725..9862
                     /locus_tag="SCK08_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1202171.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3269 a         1669 c         2243 g         2705 t
ORIGIN      
        1 ctttataaac aaagatgttc aagcgagcat cttatttttt tgtgcttaat ttgaaaggag
       61 aacatttcat gtataaaaaa caaaaaaaga aattatgtgt attaactatt ttgagtttgg
      121 ctgttcaagc aattgttgga gcaattgctc ctacgattac ttttgctgat gaaattaccc
      181 atccacaaac ggtaaccgta gaactagatt tagcacatca atatgctgtt gaaggaactt
      241 ttagtgatgg tcgtccaatg tcagaagtca ctgttccgca ttatgcagtg tacaacggct
      301 ttaaacaaga tattttctgt attgaaccag gagtcccgat ttacaatgag ttcactcctg
      361 gctatgagaa aaacccgtta ccagatatgt ccgataaagc caaattagtt tccgttttat
      421 ggaaaaaagt aggtacagat gcagatactc atattgtggc acagaagatg atctggcaag
      481 aagttaatgg gtataccctt cactcaataa aacgatcaga tggtagtgcc gtaaatatcg
      541 cagccattga ggcaaaaata aatcaagcca ttgacgatta tcagaagaaa ccaaccttcc
      601 ataatagtac ggctaaaaca gtattagggc aatcgactac tgtgacggat acgaataatc
      661 tgaatttgtc agagttcgat gaggttgtgg aaaatgccgc aaacattgat tatcgtgtga
      721 atggtaatca gttgataatc accccaaatg ccaattcaaa agaaagtggt gtgctgacgc
      781 ttaagaaatc tgctggtacc ggaacacctg tcgcctataa aaaggctggc caacagactt
      841 taatggccgg ggcgattgat aaacctaata cctacacaat caaaattgat gtggaaacgg
      901 agggttcttt aaagatcaaa aaagtcgata aagaatcggg tgatgttgta ccaggaacgg
      961 ttttccattt agactttgga aaaactttac ctgcaaaaga cgtgactact gacaaagaag
     1021 gcattgctac attggatgag attcctcatg gtacaaaagt aaccattacc gaaaaatcag
     1081 taccggcgcc ttatacgatt gataccaccc ccatgactac caccattaaa gcaggcgaaa
     1141 ccatttacgt gacttcaaaa aacgcacggg aaaaagggca aatcattctg gataaaagcg
     1201 gagttgaaac agggagtgat ctttggaatg acaattattc cttagctggt aatacgtttg
     1261 ccattcgcaa agacagtcca accggtgaaa tcgttcaaga aatgaccacg gatgaaaacg
     1321 gtcatgcgga aacaccaaaa gagattgcta atgcgttgga attgggaacc tattatgtga
     1381 ccgaaactaa agctagtcat gggttcgtga atacgttcaa acctgtaaaa gtcgaattga
     1441 agtatgccaa tcaaaccgta gcccctgtta ccagtaacgt aaaagggcaa aatcaagaag
     1501 tcactggcga aaccactttg acgaaagaag acaaagatac tggggataaa gcacaaggta
     1561 aggcagtctt tgagggtgct gaatacacct tgtttactgc taaggatgga aaagctgtta
     1621 aatggagtga ggcttttaaa ccagagttag cgaagggaac gaaaggttct gatgaaacgg
     1681 tcactttgac tttagacgaa aagaaccaag ttgccgttaa acatttagct atcaacgagt
     1741 attattggca agaaacaaaa gcgcccgaag gatatagcct tgatgaaaca aagtatcctg
     1801 tatccatcaa aaaggtggat gatagcgaaa aaaatgccgt gattacccga aatgttacgg
     1861 caaaagaaca agtgattcgc tttggctttg atttctttaa atttgctgga tcagctgcgg
     1921 gcactaccga aactggcttc aatgatctga cctttaaagt gtcgccattg gaagggacca
     1981 atgaaatcac aggtgccgaa gatgaagcga ccacagctta taacgagcaa ttagggtttg
     2041 atggttatgg caagtttgaa aatcttcctt atggggatta tttacttgaa gaagtagaag
     2101 ccccagaagg ttttcaaaag attacgccat tagaaatccg ttctactttt aaggaaaaca
     2161 aagaagactt tgtgaagagt gagtatgtct ttaccattac cgaacaagac caaaaacaac
     2221 caattaaaac ggtgaccgtt ccttacgaga agctgacgaa caaagcattt tctgttagtt
     2281 tgaaccgttt aatgctctat gatttgcctg aggaagaaga tagtttaact tctcttgcga
     2341 cttggaaaga cggaaacaaa gaattaactt ctctggattc taccgagcta gttgataaat
     2401 tgagttacaa tctacatgaa atcaaagaag actggtatgt ggtagcgcaa gccattgatg
     2461 tggatgcgac aaaagctgcc caagaaaaag atgaaaaagc caaaccagtg gtgattgcag
     2521 aaacaagcgc aacgttggcg aacaaagaga aaacaggaac ttggaaaatt cagcataaat
     2581 taaccgccga acaagtttta aataaaacta ttgtcttatt caactatgtg tatgaaaata
     2641 aggaagcttt tgaagcaggc gataagccag tagcgaagga tgttagcttg aataaccaag
     2701 cccaaactgt cagttgtaca gtggaacacc atgtttctat ccaaacaaag gcccacctag
     2761 aaaacggttc tcagaccttt actcatggtg atgtggtgga tatgtttgat gatgtgtcga
     2821 ttactcatga tgtactggat ggctcaaaag aagctttcga aacaattctg tatgctttat
     2881 taccggatgg tacaaacaaa gaaatttgga aatctggcaa gattgattat gaagtaaatg
     2941 ataaagaatt caccaaaact gtgcttgcga aaaaagtaga taccggaaaa tatccagaag
     3001 gaaccaagtt tacttttgcg gaaatcaatt acgataaaga tgggaatatc aatggaaaac
     3061 acaatgaaga tttgaaagaa aaatctcaaa ccttaacacc aaaagaagtt ccaaccatac
     3121 taagtacgcc gaaacaaccg gaaacaccaa ctgttccaag tggctctcaa gaatctagtc
     3181 ccacagtgaa gaccttccct caaaccgggg agaaaaattc caatgtttta ctgttcattg
     3241 gttttacttt gatttttgcg acggcgggct attatttctg gaatcgccgg aactaaggtg
     3301 atgagatgat acaagtaaat cctaagaacc aacgtgcgcg accgcctcct aaaaaagtag
     3361 tcgcgctatt tttgtatcca aaactaacaa tgaaaagagg aaaagaagat ggaattgaaa
     3421 tttgtcgtgc cagatatggc tgaaaccttt ggaaagttaa gttatgccgg tgaaggagaa
     3481 attataacag aaggatatgg acggaatact acggtgattg gtcgcagtta tcatttgtat
     3541 tccagtaagc aacgggccga tgatattgag gtggtagtag ctgcggaagc cggtgaaaag
     3601 gattttgatc aagaccaacc gctaaaagcc gtgaatcccc atttggttgc caaaggctat
     3661 gagattgaga atcgtgggtt tactgattac gtgttgtatg ttgatgattt agtgaaagca
     3721 taggaggaaa agaagatgag attaacagaa ggtattgtcg tagattcagg tttaacattt
     3781 gggaagttgc gtttttccgc attacgccga gaagtacgga agcaaaatga ggatggaaca
     3841 attggtaatg aggtaaaaga acgaacctat aatttaaaat cctctgcaca agggcgaatg
     3901 attcaagtta gtatcccagc taatgtaccc ttacgcgagt ttgcttatga tgcagaggta
     3961 gagttggtca atccaatcgt ggataccgtt gccaattatg tttttcgaga aggaactacc
     4021 gttaattggt tcattaaggc ggatgattta gtcttgaagc ggcagccgaa tcaaggaaat
     4081 cctacgaacc aaaatgaaag aaagaaatag gtgaagatta tggaaattaa tgttgaacat
     4141 attttagatt gtctcgatca atatggaaaa ggcgagctga ctgaggaaca actcgttaaa
     4201 gcattaacct ctgatgaaaa aatgtttctg attatacatc aaggtttact tggagaaaat
     4261 agcgacagtg aagaagactc tgatgtacta gactggttag gagaagaaga gtctttcttt
     4321 atagttgtcg aagtaaatga aaacttatgt cggcaggcag aatccatttt ggaagaaatt
     4381 ggtgtggaga tgccggatgc tattgaaggc tttttaaatc aattggttga aacgaaacaa
     4441 ctcccctcct cgtagcagta aatgactagc ataacttttt gaaattgcct aatgaaatcc
     4501 tattgggctt tttcgattgg aggtggaaac catcaaaatg tacaaaggac atcgcatacg
     4561 cgcaggagat cagcacttgg tttatcactt tgttcttggc tggctactcg ctctgttcat
     4621 cggttggatg agcgtctttt attttcaaga gtttagacaa tttgatattt ctaagctatc
     4681 actttctact attgaaatcg tttggtctat aaaagattta gtatgtctgc ttggaagtct
     4741 cgctttttca ggagctatga tattgcttta tattcacttt tttcttgatc attggcgaag
     4801 cttgtggcat cgacaaaagc tagctcggat gattctagag aaccattggt atgaagtgaa
     4861 acaaactcaa agtgaaggct ttttcaaaga tttgaatagt agccgaacca gagagactat
     4921 cagttacttc cccaaaatct gttatcgaat gaaagatgga ttactgtcta ttcgcgttca
     4981 aatttcactt gggaaatacc aagatcagct tttaaaattg gaaaagaagt tagaaagcgg
     5041 actgtattgt gagttagtag aaaaggaact caaagactcc tatgtggagt atactttact
     5101 gtatgatatg atcgccaatc gaattgggat agacgaagta gtggcagaaa atgggactct
     5161 gcgattgatg aaaaatcaag tatgggctta tgattcttta ccccatatgt taatcgctgg
     5221 tggcacgggt ggagggaaga cctatttcct cctcaccatt atcgaagcac tattgaagtc
     5281 agatgcagaa ttgtttatcc tcgatcccaa aaatgcagat ttagctgatt taggtacggt
     5341 gatgcctcat gtttattcac aaaaggaaga aatttctgct tgtgtggaag atttctatga
     5401 acgtatgatg gctcgtagca aggcaatgaa agaaatgtct aactacaaaa caggagagaa
     5461 ttatgcctat cttggacttc cacccaactt tttaatcttt gatgaatacg tggcttatat
     5521 ggagatgtta acgacgaaag aaagtgcggt gattttgaat aagctcaaac aaatcgtgat
     5581 gttgggtcgt caatctggtt tctttctgat tttggcttgc caaaggccgg atgcgaaata
     5641 tttaggagac ggaatccgcg atcaattcaa ctttcgcgtg gctttgggtc gtatgagtga
     5701 actcggatat tccatgatgt ttggggaggt cgaaaaaaac ttctttatga agcacatcaa
     5761 aggtcgaggt tatgtggata caggtggcag tgtgattagt gagttttata cgccacttgt
     5821 accaaaaggg tatgatttct tagattcaat tagtaaatta gaaaagatga atgattgagt
     5881 gatcttcaat actgacttta tacttcaagt aacgtataat acttttatat agtaggaaga
     5941 aaggggataa ttaatatgga aagtaatgat tcttatttag agtatgcaaa agcaaataag
     6001 gataagttta tttcttatat tatcaaaggg aaatcccccg aagttggacg agacgcagtt
     6061 tttatggcag ggagtcctgg ggctggaaaa tcagaggttg ccattggatt agctgagaat
     6121 tatgataacc atgtgattat tgatgcggat tactttcgca cccagtttcc tgattataat
     6181 ggaaaaaact ctagtctctt tcaaaaagca tcttcttggt tggtagagca ggcatttaga
     6241 tatgtcttag atcatggcta ttcttttatt ctggatgcaa cttttgcaat attaagtgct
     6301 gaaaaaaata tctcgcgggc gttgaaaaaa cattttcgag tgacgatttt ctatgtttat
     6361 caagacccta aaatcgcttg gaaatttaca caagaacgtg aaattgcaga gggacgtcat
     6421 gtgccgaaaa aggcatttat taacgctttt ttcaaatcaa gagagaacat tgcaaaagta
     6481 aagagtaaac atccagatgt attgcttcat atcataataa aagattatac gaataatatt
     6541 tccgaagttc attatgctac ggataatcta gaattatttt taccactagg gtatagtagg
     6601 aaagatttgg aggttcaatt acatgactga gaaagaacaa gtaacgaaaa ttgtaaagaa
     6661 atacaataag agcattgcgg atttatcgga aaatgctact gcaaaagagt tcaaaacggt
     6721 gattaaatat gtagccgatc aggccaatga aaaacaacgg aaattagttg gattaaataa
     6781 aaaatagtag gacaaattaa taatgtatga gccaagagca aaatgttctt ggcttttttc
     6841 tttgcaatga atgaggtaat gtatgagatt tttagattta ttcgccggta ttggtggctt
     6901 tcgtttaggt atggaacagg cgagccatca ttgtatcggt ttttgtgaaa ttgataaatt
     6961 tgctagacgt agttataaag caattcatga tacaagtaag gaggtggaaa tgcatgacat
     7021 cacaagtgta tcagatgagt ttattcaatc cctcggacca gtggacatcc tttgcggcgg
     7081 atttccgtgc caagcttttt caattgcggg aaagcggcaa agattttccg atactcgagg
     7141 tactttattc tttgaaatcg ctcggttcac cactcttctc cgacctaagc ttttattcct
     7201 tgagaacgtc cgaggattgc tcaatcacga aggaggggct acgttcgaga cgatcctccg
     7261 aacgctggat ggattggggt atgacgtgga gtggtaggtg cttaactcaa aggcctacat
     7321 tccccaaagc cgggagcgga tattccttat cggacattct cgagacgcat gtaccgaaca
     7381 agtatttcct atcattggac cgtcttcaac atctgatcaa aacatcagaa atttggtaaa
     7441 tatcaatcct tccaatcgag gaatgggtgg acaagtctat ggttcagatg gcgtagcgcc
     7501 aaccttaaca ggtgatgaag gaatcaaaat cgcactgcca gcaagggatg gaatctctgt
     7561 agcaggacta ttaccgggaa agtttgaaca aggtaatcga gtttatgaag ttactggaac
     7621 cgcccccacc ttgtcaacga aacaaggtgg tacaaaaatc atgattcgaa aaaatgaaac
     7681 agaatatcaa gaggtgaagc cagaagacag tgtgaatctg gcttttccta attccacaag
     7741 tcgccgaggt cgtctgggaa aacagagtgt tcatacctta ctaacaggag accaacaagc
     7801 gatcgtcacg gatcagtatc aaattcggaa gctaacacct agagagtgtt ggcgacttca
     7861 gggctttccc gattgggctt ttgatcgaga ttctcaagtc aattcagata gtcagttgta
     7921 taagcaagcc ggaaactctg taacagttcc ggtcattttt gatattgcca gaagattaaa
     7981 agagttggat gtacatggtt tatgatgagt tgattggaga aatttattgg gtgattggaa
     8041 agattcagtc agacccagaa ttagaagaac aattgcgacg gttaaaattt gatattcgca
     8101 agaatggtgt caaagtcgct ggcgatccat atttaatgaa tgaggagacg gatgctcgaa
     8161 ttgaaataaa tcaagtaatt gcagaatttg agcggattgc ggaccaagca aaagaaccag
     8221 atattcgtca atacttgtta gaaatgaaag cagagttgga agtaaatggt attacagatg
     8281 agtaacctaa accaacacaa ctgactgtta ggcgctgacc gcggaggatt cgcgaagcag
     8341 aaatcctccg tggtgctgtg cctgttcatg gacggcgtta gccgaccatg atgtctcacc
     8401 cccgtatcta acaggggggt acaaaaacac aaaaaataag taagcaacca ttaagaatgc
     8461 ttgttgtatc aaagatagta agtaatttgt ataggtgtta actcatttga aaaagttaac
     8521 accttgaatt tggaggtgag aacttggcac aacggaattt agattatcgg ctattaaaag
     8581 accgacgcaa cgaatacggc gtttcacaaa ataaattggc tacggcttgt ggacttagtc
     8641 ggccgtactt aaatcagata gaaaatggtg gcgtaaccgc atctataaaa accatgagga
     8701 aaatttttga ccaactggaa agctttaatc ccgatttgcc tttgacgtta ctgtttgact
     8761 atgtaaggat tcgctttccc acaacggatg cacgaaaaat tattcaagaa attctccatt
     8821 tgaaatttga gtatatgctc catgaagatt atgcctttta ctcctatcaa gaacagtatg
     8881 tcatgggtga tattgtggtg atgttgtctc atgaagaaga taaaggtgtg cttttagaat
     8941 taaaggggcg aggttgccga caatttgaaa cttttttact cgctcaaaag cgtagctggt
     9001 acgacttttt cgaggattgt ctaaaggccg gtggcgtgat gaaacgcttg gacttggcaa
     9061 tcaatgatcg agtggggcta ttggatatac ctgagctaac gaaaaaatgt cagaaggaag
     9121 aatgtatctc cttgtttcgt acctttaaaa gttatcgttc cggggaactt ttaaaggctg
     9181 atgaaaagga cggcatgggc aataccttat atatcggcag tctcaagagt gaggtttatt
     9241 tctgtttgta tgaaaaagac tatgaacaat atatcaagtt agggattcca ttagaccaaa
     9301 ctgagacgaa aaatcgtttt gagattcgac tgaaaaatga ccgtgctttt catgcgattc
     9361 aagatttgtt aaaaggtcgt agcattgaga gcaccacgtt ttccattatc aatcgttact
     9421 tgcgatttgc ggataaagta gaaggtaaaa ggcgaaccaa ctggcccttg aatgaacaat
     9481 gggggcgttt tattggacgg aatcggaaag agattcaatt aacttccgag ccaaaaccgt
     9541 ataccattga acgaaccttg aattggctag gccgacaagt cgcccctacg tggaaaatgg
     9601 ctaaggagtt ggatcgattg aatcggacaa cctatatcca ggatatggta cagaatgcac
     9661 gcttatcaga ccgacataaa aagattttgg agcaacaaag tatggcaatt gaaaatttga
     9721 ttatatgaaa agaggaatcg tcatgggaat tgtaaaagat atttttttgg tatttggtgg
     9781 cagtatgctt ggtgttactg tgatgtgctt gatgaatgca agtgcagcag cggatcgagc
     9841 aatggaaaag aaaggaaagt gacaagatga atttcggaca aaattt
//
LOCUS       DARBFD010000096         9879 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000096 DARBFD010000000
VERSION     DARBFD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.098"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(343..567)
                     /locus_tag="SCK08_002326"
     CDS             complement(343..567)
                     /locus_tag="SCK08_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202172.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            758..3535
                     /locus_tag="SCK08_002327"
     CDS             758..3535
                     /locus_tag="SCK08_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1202173.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3616..4035
                     /locus_tag="SCK08_002328"
     CDS             3616..4035
                     /locus_tag="SCK08_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEG1202174.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4047..4517
                     /locus_tag="SCK08_002329"
     CDS             4047..4517
                     /locus_tag="SCK08_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1202175.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4534..5313
                     /locus_tag="SCK08_002330"
     CDS             4534..5313
                     /locus_tag="SCK08_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1202176.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5300..6121
                     /locus_tag="SCK08_002331"
     CDS             5300..6121
                     /locus_tag="SCK08_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1202177.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6138..7187
                     /locus_tag="SCK08_002332"
     CDS             6138..7187
                     /locus_tag="SCK08_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1202178.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7200..8204
                     /locus_tag="SCK08_002333"
     CDS             7200..8204
                     /locus_tag="SCK08_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1202179.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8837..9856
                     /locus_tag="SCK08_002334"
     CDS             8837..9856
                     /locus_tag="SCK08_002334"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1202180.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3312 a         1641 c         2066 g         2860 t
ORIGIN      
        1 taatagtgga gaaaactatg gtttggcgca gattctatca ggtttgatgg aacgaagaca
       61 acctcccgac atcaagtcaa aagctacttg agattttgat ctgaaaataa aaaaagaaaa
      121 caaggtagga acatcaatgt ttctaccttg ttttcgtaat caatgggaaa aaactaagag
      181 tggtgtacgt aatcaataaa aaggatggtg acaacagtac ctctttactc tctgaaagca
      241 tcataactcg tttttcctca ttatgccaat aaattgttgt tataccacat ataaggattt
      301 gtaatagcta aaaacagaca ggaaagttga aatctgtgaa tcttattttg tatggaagga
      361 tttatagaac gcaaaaatca agataaagat gaataaaaat cctgtttcgg ctatatattg
      421 cgtatctacg ccaataaaac gagtttgttc tgtcatagcc atataaagca aaggcaaaaa
      481 tacaactaca gcgagataag atccgatgat ttctcgaaaa gttttccagg aagtatgttt
      541 cctgttttta tatttatccg atttcattga atagtcatag cctccttatt ctttcatatg
      601 ttgaaattcc agtataaaaa ctggaaggaa ctgtacgttt tttagaataa aaattataag
      661 ggataagtag gctgctgtct ataataagat atatttttca attctttatt aaagatttta
      721 ttgacaaaat gaatccgttt tctatgatgg aggaggggtg aataagttgt tgaagaagga
      781 aattttatgt tacttagaga accagacagc ctttttcgat ttagaacata tgaacgaggt
      841 ctttacagca caaaggttag ctgatcattt tggggtaaaa agaaacacag tcagtcatta
      901 tttgaaccag ttaaatgatg agcaactact agtcaaaatc aattctcgac cagtgattta
      961 tttccacaaa gaagcttttg aaaaacaatt ttttaagtta cgcacgaatt tttatttttc
     1021 tatcgatgat ctcaaagagg aacagccttt tttcgcgcaa ccaaaagact tattctcttt
     1081 tatgatcgga tacgacgcca gtttgaaaga aagtatcgaa caaatcaaaa cagctcttta
     1141 ctatccagat ggcggattac cacttttgat tacaggcgaa agtggcacag gtaaaagtta
     1201 tttagttaat ttggtctatc agtattgtct gcttcatgat ctgctagaag attctgcacc
     1261 attcatcact tttaattgtg cacaatatgc agataatcct gaacttttga ctagcaattt
     1321 gtttggacat gttaagggag cgtacactgg tgcagaagaa aataaaaaag gggcttttga
     1381 agcagcagat ggtgggatct tgtttttaga cgaggtacat cgcttaacac cagaaggtca
     1441 ggaaaaactg ttcacctatc ttgatcaagg tgtgatttat cgtatgggag aacctaattt
     1501 gtcacgccgg atcaaaacac gtctcttttt tgctacgact gaagagatta ctagtcattt
     1561 cttaacaact ttcattcgaa gaatacctat ccgaatcgaa ctacctgcac ttaatcagcg
     1621 tagtcgtaat gaacggctag aactgattta ctcttttttt ctcgaagaac aaagaaaaat
     1681 ggggagccct ttgtcagtca gtggaccagc tttgtcactt ttaacaagtc agaaattatc
     1741 cggaaacata ggagagctga aaaatatcgt caaagtttca gcagcaaaag catatgctga
     1801 acagcgtgaa cagcctgaaa tccatgtcac gatccatcat ttgcaaaaag aactacttgc
     1861 gcagcctatt actaaaaata cagcacagca aggaatctat attacgcaag accagacctt
     1921 agagcaatta atggagaagc aacagcccga acagcagcga atcgttaaaa gttttgagca
     1981 aatcctaatt gatttcaaga aaaataactg tctgctatat tcttgcgaag gaaaattaaa
     2041 gcaagaagtg attcaattat ttgatttttt gctgtttgag accagtagac aagaaaaaca
     2101 cgagctgctg gtttattgga cacaatctat ccgagagacc tttcgacaaa tggaaactgc
     2161 ctatcaaatc aaattcgatg gaaacagtgt ctatgccttg agttactatc tctatcagcg
     2221 aagcactatc aaatggttac cggaagaaca tgatattatc caactaatca aagaattaga
     2281 acaacaagtt tctcagtctt atccatctag ttatcactat gtccaacgaa ttctggaatt
     2341 aagtaagatc aaattagata ttgaagacac aagtatggat cggattttac tgacgatcta
     2401 tctaaaaaaa gcgaaatggt caaaagaaaa acgactgcca aaagcgatga ttgctgctca
     2461 cggttatgca actgctagca gtatggcaaa tgtagtaaat cgtttattac aagaggatct
     2521 atttgaatca tttgatatgc cactagatgt cacaccacag cagattgctt cagaaatcct
     2581 tgattattgc gaaaacagcg atgtttcaaa tggattgatc atattagtag acatgggatc
     2641 tctgaaagaa atccatcagt tttttaaaaa acagttatcc gtaccgcttt tgattttgaa
     2701 taatgtgacg acacctttag caatcacagt aggggaatgc ctgcaaaaaa atgtatcatt
     2761 agaagagata gcagaagaga ctgttcgaca gatccagcca gaatggcgac tgctctatcc
     2821 agaggaaaac aaacccaaag cactcattac tacttgcttc actggaatcg gtacggcggt
     2881 ccacttaagt gaactgctag aaaaaagctt acctacggct tgtcagttga aaatcatccc
     2941 atatgaatat cagcaattaa aagataagaa aaacagtgac ccattatttt ccatatacga
     3001 agtcgtagga atgattggca ccacagatcc tgatatcacc aatatcgact atctttcatt
     3061 agaagacctc atctcggggg aaaagatgtc tgtactagct gaatggttgg acactactat
     3121 gaatgcttca gagaaggaaa tattcaatca gcgcatcatt cgtaattttt cattggaaaa
     3181 agtactggat tccgtgacta ttctagatac agagaaggtg atgggagaaa tcgatcattt
     3241 tatgagagaa ttagaggtgc agttgaatcg aagcatcagc aatcccaaaa aattagcgtt
     3301 gtatgtccat gtgagctgtc tcattgaacg attgatccga caaatgccaa tcgaaaccta
     3361 tcaaggatta gagaaatgga agcagtgtca aaaagaaggg ttatctgcta ttaaatctgc
     3421 gtttagtgtc attgaagagc attatagtgt catgatcccc aattctgaga ttgcctatat
     3481 ctatgatatt ttgagtgaag atacagagtt tctctcgata gaagaagaat tttgaacata
     3541 agcaagttgt tgccactaat tttggcatgc aacttgcttt tttagtatgt gtaagtgaaa
     3601 agaggagatg aaaaagtgga tagagaaatt atcttagctt cccatggaaa atttgcttca
     3661 gggatattag attcattaga gctgatctat gggaaaaatc attctattac tgtattcgat
     3721 tgctatactg atgagaattt tgatttagcg gaaacagtaa ggcagctttt tatgaggtac
     3781 aaagataaag aaatcattgt tctgacggat ctttttggcg gaagtgtcaa caacgaattc
     3841 ttacaatata tcaatcggga tcatgtctat ctcgttgccg gattgaattt gccactattg
     3901 attgagctag tatctcggtt ggacgctgaa gtagagacaa ctgttttgat ccagcaggtg
     3961 ctaactgatt caaaaaaaat gatccagttt tgtaacgagc ggctcaatca gaaaatagaa
     4021 gaagacgaat tttaggagga agaaaaatga ttgtattgac aagagtcgac catcggttat
     4081 tacacggaca agtagctttt tcatggacac aaacaatagg cgcagactgt attttgattg
     4141 ctaatgatga tgtacctacg aatgagatac gaaagacaac gatcaaatta gccaagccac
     4201 aaggagtcaa actagtgatc aaatcaattg atgactcgat tgccgcgttg aaaagcgggg
     4261 tgacagataa atataagtta ttcatcgtag tggaatcgat cgaggatgca tataagctgg
     4321 cagaagcgta tccagaaatc aaaaccatca acttaggagg gatcaaggca aaacctggta
     4381 ctcgcagcat cggaaaagcg gtaaatattt taccagaaga ggaggaacgc ttgaaacgat
     4441 taattgacaa acaaatagaa gtagaaatcc gtcaagtacc agcagataaa aaagtggatg
     4501 taagagaagt tttgtaagaa aggaagaacc tcaatgattg tacaagcagt cttattagga
     4561 attgttgcat ttgtcgcaca atcagaatat gcactaggga catcgttgct ttctcgtccg
     4621 attgtgacgg ggctgtttac aggaatcgtg ttaggtgatg taaaaactgg gatcattatg
     4681 ggcgcaactt tagagttagc atttatcgga tcgttctcag taggtgcttc gattccacct
     4741 gatgttgtga ccggtgggat tttagggacg gcttttgcta ttacagctgg agccggtaca
     4801 gaaaccgcgc ttttactagg tttgccgatt gctacgttga cattgatatt gaaaaatatt
     4861 tacttagggc ttttgattcc aattatgaac cataaagcag acaattatgc agaagaagga
     4921 aattataaag gagtcgagca tatgcacttg cttgccggta tcggtctttc cttgatgctt
     4981 ggtgttgtgg taatggtttc ttatatggtg ggaagcaata cgatcggaaa tctattaaat
     5041 atgattcctg acttcgttca aaaggggttg tcagtagcta cagggttgat tccagctctt
     5101 ggttttgcga tgcttgctcg tttgctgatc aacaaacaag ttgcaccata ttttttctta
     5161 ggttttgcta tagcttctta cttagagatt cctgtgaccg gagtagctat ttttggtgcg
     5221 attttagcag ttgtggtagt caatattatg aatgtccgtc aactaccact tcaaacaact
     5281 gcaggggagg tagaagaaga tgaagaattc tgagatcaaa aacagcaaaa taacgaaaaa
     5341 agagctaaat cacgtatttt ggcgttcttt tcaaatggag ttttcttgga attatgaacg
     5401 tcagatgaat atggcttatg tttatgcaat gattccgatt ttgaaaaagt tatatcagtc
     5461 gaaagaagaa atggcagcag ctttgaaacg gcatctagag ttttttaaca cgaccccaca
     5521 tatcgttaca ctgattttag gaatcacggc tgcaatggag gaagaaaata aggatgatcc
     5581 tgaatttgat gtgactgcga tcgatagtat caaaacatct ttgatgggac ctcttgcagg
     5641 gttaggagat tctttctttt ggggaacttt gcgattgatt gccacaggtg tgggtacctc
     5701 tttagccatg caaggaaata ttttaggccc tatcttgttc attttgattt tcaatattcc
     5761 gcacattctg tttcgctatc tagcaacagg ttggggatat cgcttaggaa cgggtttttt
     5821 gaaaaagatc caggcaaacg gtatgatgga aagtttgaca ttaggcgcgt ctattattgg
     5881 actgatggtc gttggagcaa tgactgccac gatgatcacc atcaatattc ctttaaaaat
     5941 cggttcagga gaaaatgctg tgactgttca aagtattttt gatgatatcg ttccaaatat
     6001 cttatgtctt ggagcatttg gcatagtgtt ttatctattg aagaaagaag tgaaaccatt
     6061 aacgatcttg atcggactag ctttctttgg aatttttggt tcgtggatag ggatttttta
     6121 aaaaaggagc agagaaaatg tttacgatgc aagattatat ctatgaagaa aaggaagtac
     6181 taagtacgat cttgaaaaag aatgattttt cgacaagaga acatatgaaa aaaacagtga
     6241 atttactgat cttagcaacg ggttcatctt ataacgcttg tttagcagcg aagccagcat
     6301 tggaatctta tggtgatctt actgtcgata ttcaagaacc tttttatttt ttcaattatg
     6361 gaaaactttc tccttcaatc gatactgtga ttgctgtgtc acaaagcggg aaaagtgctt
     6421 ctactgttga agcagtaaaa atgatacaaa aacaagggtt acctgttgtt gctatcacaa
     6481 atgacgtgca aagtccactt gctttagaag ctgcacaaat tattgattta ggtgccgggg
     6541 tagaatcagt aggcttcgtt acaaaaggct attctgcgac tgttttacag ttactactct
     6601 tagggatagg aattggaata agtaaaaata aaatcagcaa aaaaatagaa caagactaca
     6661 tgcaacagtt gcgcaaaata atcaaccatt taccagcgat tattcaaaaa acggaagaat
     6721 tttttgatgg atatcaaagt ttatttcgtt tagcacagcg tttcgtcacc attggatatg
     6781 gtccaaactg gggaacagct aaagaagcag agacgaaatt gactgaaaca atccgcgttc
     6841 cgtcacaagg atttgagtta gaagcttata tgcacggccc atacctggag gcagatgctt
     6901 cgcatttgct attctttatt gaaggggaaa gtgtaaataa ggaacgttcg caaaagttgc
     6961 aaagatatat gagtcggtac gttggagaga ctctcaccat taccacaaaa aaagcaagaa
     7021 acgagaaaac acttggatta gcaatagaat gtgatgaata tctgtctgta ttagcgcttg
     7081 tcgttccgtt tcaactattc gcttataaaa ccgcagtagc taaaggtatt gacttgaaca
     7141 aaaagatttt tgaagacttt gatacagtct tgaaaagtaa attataagag gtggaaatca
     7201 tgctgaaatt caatgaagaa gaacaaatca aagcaataaa aggcgcacta gctttacgtc
     7261 cgcaagtaga agaaatcatc gataaacttt atgcggaaaa atttgacgct gtctattatc
     7321 taggaatcgg tggaacgtat gcttcctcga tgcaagctgt cacttatatg aatgggaaaa
     7381 gcaacttgcc tgtctttgta cagcatgctg cagaatatta cactactgga aacaaaagat
     7441 taacaaaaga ttctatcgtt attttgtcct ctgttaccgg tacaactcaa gaagtagtga
     7501 aagcagtaga acaaatcaag aaagtaggag cgacactgat tggatttatc gataaagcga
     7561 acagtaagtt gtctcagcta tgcgattttg tggtcactta tccagctcct ggaacagaac
     7621 aaatcaaatt ttttatggca gcagatcgat tgatgtattt gaatggagaa ttcgaagctt
     7681 attcagaata ctatgaacag ctcgaacgtt atttaccaac agggttagtg gaagcagaga
     7741 aaaaagcaga tgcatttggc ttatcatttg cagaaaaaca tcgacgtgat agtatgcatt
     7801 attttattgg agcagggaat caatggggag ctgtttattc atatgccatg tgttactggg
     7861 aagaacaaag ctggcttcca tcgaaatcta tccatgctgc cgagtttctt cacgggacgt
     7921 tagagatcgt agaagaaaca accccagtca cattattttt aggcgaagat gaacagagag
     7981 tgttatctga acgtgtggct aaacttttgc caaaaatttg cagcaattat acattgatag
     8041 acacaaaaga ctatccagta gaaggtatca gtgaaaaata tcgcggacgt gttttgtctt
     8101 tcttattgat gcatgtagtg acacaacgca tcgatgctca tgtggagcag ttgaactgcc
     8161 atccattaga gatacgccgc tactatcgtc agtttgatta ttaggataga ctggataaaa
     8221 agaatataaa aaataaagag agcagcgata actgctccct ttattttttt aaaatattgg
     8281 catcaattac gttataaaac acattttttg cattatctac agttcaactt ataatctgtt
     8341 taatgggaca tgtgggtgcc aaccattttt tgttatgcaa tagtactatt tagttgttgc
     8401 atggatccgt agtaaaagtc caagtacaac gagttgcgcc agtttacata gcgttttgta
     8461 tccagaatgt tgactaaaac aaaagttcct atgtcaaccg tcaacagaga caatctgtcc
     8521 gttgcaggac ctgacagatt tagaaatgtt caatctgtct cttatgtatt ttaaatcaat
     8581 actcactagg agaagtttcc taacctccta atagtaatgc tggtagtaac attgtcaaaa
     8641 ctgcttcctc ttaggaacaa gcaaagcata acgagtgtaa aaataaaatg tagactattg
     8701 taacatatca ttgattattt caagtaaaaa cgataaaaga agtaaacaaa cacttaaaaa
     8761 caatggaaat tagtcccaac tttaagaaat gtatactata atggaaatta tcaattattt
     8821 atttggagga atatccatga aacaaaaaat cgcagttctt ggtcctggat cttggggcac
     8881 tgctttagca caaacattgg cagaaaacgg gcatgatgtc cgtatttggg gaaatgtacc
     8941 tgaacagatt gatgaaatca ataagtatca tacaaatcag cattttttac ctgatttgac
     9001 gatcccagaa tcgattatcg gatataaaga attggcagaa gcagtggata acgcagatgc
     9061 tattttattt gttgttccaa caaaagcgat tcgttcagtc gcacaggaac ttattggtaa
     9121 aatgaatact aagccaatca ttatacatgc aagtaaagga ctagaacaag atacgcataa
     9181 acgaatctca gaagttttgg ctgaagaaat tccagaagaa aaaagacagg caattgttgt
     9241 attatctggt cctagccatg ctgaggaagt ggcagtacat gatatcacaa caattacagc
     9301 tgcaagtatt gatcaaaaag cagcagccta tgtacaaaaa ctgttcatga atgattattt
     9361 caggatttat acaaataatg atgtgattgg cgtagaaact ggtgcagcac tgaaaaatat
     9421 cattgcaatt ggtgccggcg ctatccatgg attaggattc ggagatgatg caaaagcagc
     9481 aatcatgact cgaggattgg cagaaatcag ccgtcttggt gtcgcgatgg gtgccaatcc
     9541 tctgacattt atcggtttga gtggtgtggg ggacttgatc gttacttgta caagtgttca
     9601 ttctcgcaat tggcgtgccg gtaacttgtt aggacaggga cataagctgg aagaagttct
     9661 agaaaacatg ggaatggtcg ttgagggtgt ggcaacgaca aaagcagcag tagagctagc
     9721 tcaacagcta ggtgttgaga tgccaattac tcaaacaatc tataatgttt tgtataatgg
     9781 agaagatatc aaaaaagcag caaaagaaat catgttacgt gacggaaaaa tggagaatga
     9841 attttctctt cgttaaatag atgatcaata gttaatcgt
//
LOCUS       DARBFD010000097         9466 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000097 DARBFD010000000
VERSION     DARBFD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.099"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            81..154
                     /locus_tag="SCK08_002335"
     tRNA            81..154
                     /locus_tag="SCK08_002335"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:115..117,aa:Ile,seq:cat)
     gene            complement(539..1036)
                     /locus_tag="SCK08_002336"
     CDS             complement(539..1036)
                     /locus_tag="SCK08_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1202181.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1076..1885)
                     /locus_tag="SCK08_002337"
     CDS             complement(1076..1885)
                     /locus_tag="SCK08_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1202182.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2219..3076
                     /locus_tag="SCK08_002338"
     CDS             2219..3076
                     /locus_tag="SCK08_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1202183.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            3069..3971
                     /locus_tag="SCK08_002339"
     CDS             3069..3971
                     /locus_tag="SCK08_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1202184.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            4004..4354
                     /locus_tag="SCK08_002340"
     CDS             4004..4354
                     /locus_tag="SCK08_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1202185.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4403..5326
                     /locus_tag="SCK08_002341"
     CDS             4403..5326
                     /locus_tag="SCK08_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1202186.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5404..6297
                     /locus_tag="SCK08_002342"
     CDS             5404..6297
                     /locus_tag="SCK08_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1202187.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6335..6898
                     /gene="amaP"
                     /locus_tag="SCK08_002343"
     CDS             6335..6898
                     /gene="amaP"
                     /locus_tag="SCK08_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1202188.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6912..7100
                     /locus_tag="SCK08_002344"
     CDS             6912..7100
                     /locus_tag="SCK08_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1202189.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7113..8015
                     /locus_tag="SCK08_002345"
     CDS             7113..8015
                     /locus_tag="SCK08_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1202190.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            8047..8289
                     /locus_tag="SCK08_002346"
     CDS             8047..8289
                     /locus_tag="SCK08_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1202191.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8545..8916)
                     /locus_tag="SCK08_002347"
     CDS             complement(8545..8916)
                     /locus_tag="SCK08_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202192.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3320 a         1619 c         1825 g         2702 t
ORIGIN      
        1 ttacttatat tttgaaactt gtgagagttt gtcattcgtg ctatagttga aaagtaacaa
       61 aaataaattt tatttatgat gtacctttag ctcagttggt tagagcagac ggctcataac
      121 cgtccggtcg taggttcgag tcctacaagg tacataagag gttgtgaaaa agctgccctg
      181 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttact tggctgttct
      241 tattactgca gatgcaagct tttgaatacc gtttaaggaa gaggttgtga aatcagcgtt
      301 ttgacctgag tattagctta acaaacggaa aaatagttcc tcatattttg ccgtttgtta
      361 agctaatacc gagggtcagc tgattgaaca ccatttaaga aagaggtcgt gagcctgccg
      421 tttcactctg tgtcataaaa aagccaatct actttgatag aaaaagcacg taggactaaa
      481 acagttctgc gtgctttttt tattgtgtgt taaatatcgt aaatgaagat agatattttt
      541 atgattcgac gatagagacg ttgaaatctt tcaacagctc taaagcggat tctccgtgtt
      601 ttctactgga gactaacatt ggatcaacaa aaaagtctgc ttcaggaaaa gcactctgca
      661 ggcctatcag attagagagt acacacaagt ttgtttcaat cccgactact tcgatctctt
      721 tttgttcttc tttgctttca aataaagttt cctggatttc agctaaagtt tcaggtggaa
      781 tcgtaaagta atacttttta atcactcgtt cattttcttg tggagaaaga agagggatca
      841 attgcaaatc ttctcgttct tcttctttgt ccttcagttc aactggtata tcccgtgtga
      901 agagaatata ttcattattt tggcgtgctt tagctaatct ttgagcaata cgttctgcca
      961 attgatctga ctctggaata taaaattcgc tgtttggatc aagaatatga ttttgcatat
     1021 caataataac taacattttc tttcctccta cattcatatc gtcattttat tctttttaat
     1081 agaagttttt taaaatataa gcaacaccag cttcttcatt cgtcagtgtt acttggtttg
     1141 caatttcttt tacttgtcgc tgtgcattcc ccatcgcaat actcaacccg ctgtaattca
     1201 acatagcaat gtcgttttct tgatcgccaa aagccatgat gtcactcgat tcgattccaa
     1261 ggtaatccgc cagaaaaaca agtgaatgag ctttagtgat acctgtaggc gtgatttcta
     1321 agaaatgcgg atcagagaat actgattgga taccatctgc ttgcagatct ctttgtagct
     1381 tctgtagttt ttcttcactc ttatctgtta atgctaattt aagatagcta agagaaggat
     1441 cttccaacat atttaaaatt tgatcaaaag aatggttctg taaattgaaa tgttcataat
     1501 aagggtcatc tttgactaga tttgattcat cgaatgtcag ctgatcatct tgggtttctc
     1561 ggctagcaat gatggatatt ttcgcttctt gagcatagaa gaaggctttt ctcagtgtat
     1621 cgctatctat ttttgaacgt ctgagaactt tcgccatttt tccatccttc atttcagcaa
     1681 tcagtgaacc attggaagaa atcaaatagc caccgattcc taattcggaa aaaatatctt
     1741 tagcagaaat gctgttcctt ccggtagcga cggttacgat tccgccattt tcatcaaatt
     1801 tccgaagtgc ttggatattt tcttctggta tctttccatt tcgatcaagc aaggtaccgt
     1861 ctaaatcaat cgctgctagt ttcattttat cttcctactt tctttttaca tatctatttt
     1921 cctgttacct taagattaaa tgagaattta aaaaaatgaa actaacttgc tttgtcatta
     1981 tgaatttttt caaaaaacat tcttaagaaa aaggtggcta tatatagtag tttttggtca
     2041 atacctttga ataaaaagcc atgaaaaacg aatagttagc gcttaccgat gcgttttgtg
     2101 ataaaaaaat tttaaaatat tcaatgaaaa caaagcatat attttgcttt aaaaccaaat
     2161 gaggaatatt ctagatgtag aacaggttag aaaaagatta gaaaaaggag tgattttgat
     2221 ggagaagaga tacggcgtag ttcctgtggt aactgcttca gacgaaaatt atgctcctta
     2281 cttgagcgtg atgatcgcaa ctgcattaga aaattgcaat aaaacaagac gcataaaatt
     2341 ttatgtgatt gacgatggtc tatcagaata tagtaaagaa ggattggaag aaacggtaaa
     2401 taaatattca agtaatgcaa gtatccaatt cctgacagtg gaaaaagata tctacgaaga
     2461 cttcctagtc agtgaccata tcacaactac cgcttactta agaatttctt taccaaactt
     2521 attagctaaa gaggattata agaaagtcct ttacttagat tcagatgtgt tagtactaga
     2581 cgatatcgta aaactatatg atgagccgtt aaatggaaaa acaatcggcg ctattatcga
     2641 tccaggccaa gtgaaagctt tggaaagatt aggaatcgat tccgatgact tgtacttcaa
     2701 ttcaggagtg atggtgattg acattgatca atggaataaa aaagaaatta cagaaaaaac
     2761 tatccattac ctcagtgaaa atggcgatcg aatcatctat cacgaccaag atgctttgaa
     2821 tgctgtactt tatgaagatt gggaacaact tcatccaaaa tggaacatgc agacttcgct
     2881 gatttttgaa cgtcatccag caccaaatga aaaatatgaa cgtcagtata aagaaggaaa
     2941 tgaaaaacct tcgatcgttc actttacagg acatgataaa ccttggaata cgttgaaaga
     3001 tcatccgtat accaatttat acttaaaaaa attagcacac agtactctaa cgaaagtagg
     3061 cgagatcaat gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca aaattcgtac
     3121 cacatttagc tgcattattt gtctcagtat tagataattg caacccgtct aaatttgttc
     3181 ggttctatgt gattgacgat gatatcgatt ttgaaagtaa acagctttta cgcttctcag
     3241 tgaaaaacgc acgaatgaac tcagatgtgg aattcttgaa aatcaataaa gaattcttta
     3301 caaatgtggt aatcagcgat cgtattccag aaacagctta ttatcgtatt gcgattccgg
     3361 aattgttccg cggcacagaa gttgaacgta tcttatatat ggattgcgac atgatcgctt
     3421 tacaagatat ctctaaatta tggcgtcttg acttcggtga ttcaatcgta gcagctgttg
     3481 aagacgctgg gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa tcaatgcgtt
     3541 atttcaactc aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa aatattacgc
     3601 aaaaagtatt agatttcatc gaacataatc cagaaaaatt acgattccat gaccaagatg
     3661 cattaaatgc tatcttacat gaccgttggt taccacttca tccgcgttgg aatgcacaag
     3721 gctatatcat ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt gaatacgaag
     3781 aaactcgcaa caatccttac attatccatt tttcaggaca tgtgaaaccg tggagcaagg
     3841 attttgaagg gccaacgaag aaatattacg aaaaatatgc agggatgacg gctttccgtt
     3901 gtgtagctaa attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa acagatgtag
     3961 ttgttaaata atagaagaca acaactgaag agggggagta ggaatggaaa ttacaatgtc
     4021 taaaaatgca gtagagacgt taattgaaaa ggttggggaa aatacaaaaa tcgcactcgc
     4081 gcttatcaat gattccgatc catttctaag ggacaaaggt gcttttgcaa aaggcagctt
     4141 ttttcaaatc attccttttg tttcggaatt tggagaatat gcgactaaaa tcgagcatcc
     4201 attattggac atctatacat ctaaattaga gcaaaattat ttcggtaaac gattgaacat
     4261 ggacttcaac aaacagcttg acagcttttc attagaaaat gaaatcgcag tactcgatta
     4321 taatatcaaa ttaaagaact gcttcttcag ctaatagttt cgagaaagtc caaaaaggag
     4381 gtatacattg agatactact ttgtggatga agaaaagaat gtaatagaaa cagatgaaaa
     4441 aaaatataat tggctcgtac ttgatagtgc aaagcccgaa gagattgagc aagcaatcga
     4501 agaattcgag cttcctgatg atattttcgt aggaaccgaa tatccggaag aagtttctcg
     4561 gatggagcgc ctttatgaga cgaaactaga gcatccattt tctttggtag tgattaatct
     4621 agatcagtca gataatcgta tcgaaaaaaa attaacgcca atatcttttg tactttccga
     4681 taatctcttg attacttgta tcgatagaaa aaccgaattt gtggatcggc tgctgacaaa
     4741 gcatggaaat catttaaact cgtttgaaaa gatcatcacg tatacattat tagatattta
     4801 cacccattac gtgaaagaac taagagagat gaaaaagcga attgacgctt tggataaagc
     4861 agctcgtaaa acaacggaaa atgaagaact gtataaacag gcagatcttg aaagagacat
     4921 cgtatatatc gatcatacct taagagatca acgggaaaca atggatcaat tatggaattc
     4981 tgaaactttc atcaaacgtt tagacgatga acagctactc tatgatgtcc atttgagaca
     5041 gcgacaaaca gaaaaaatga tcgagatcta tcgagattta ttagagagta ttggtgatct
     5101 attcaacggg atgatggaca ataatctaaa tcatatcatg aaatatctag attcagctgc
     5161 attaatcatt tccgttccgg cgttgttcgc agggatttgg ggaatgaata caggaggatt
     5221 accgggtaaa agttctgctc taggctttat actcgtagtc tttggctctt tgatagcagc
     5281 agtagtttta ggttaccatt tataccggaa agattataca aattaaagaa tctatttaaa
     5341 acaggtatca aacaaagaaa tttgttttgg caatacaatt taaaaactag gaggaataca
     5401 gcaatgacag aaccaaaatt aatcgacccg cgaaacttgt accatacgga gaaatttcct
     5461 aaacaagatc aggatacacc agctttacag cagaatatga ttcctgttcc tgattgtgga
     5521 gaagaatcct atgaaggcaa taatcaacta gaaaaccgcc gtgtactaat cactggtgga
     5581 gattcaggga tcggtcgtgc agcagcgatt gcatttgcta gagaaggggc agatatcgct
     5641 cttcaattct tcccaggaga agaaaaagat gcagaggaag tagcaaaata cgtgaaagat
     5701 gcaggacgta agattgtctt actacctttt gatcttcgtg acgaacaagc acctaaagaa
     5761 atcatagata aagccgtgag tgaacttggc gggctagata cgatggtact aaacgcagga
     5821 cagcaaattt cttgtcagtc tattgctgag ttaccacttg agcaagtcaa agacacattt
     5881 atgataaaca tcatcgctat gtttgcttta gtgaaagaag ctgttccgca tatcccagca
     5941 ggaggagcaa tcgtaacaac aacttctgtt caagcattca atccaagcga acatttatta
     6001 gattacgcag caacaaaagc ttcaatcgct aactttacga tcggtttagc aaaacagctt
     6061 gcacctaaag gaatccgtgt aaatggtgta gcacctggtc caatctggac accattacaa
     6121 ctagacgaag gacaaccttc tgattcgatc cctgaatttg gtcagcaaag tttactagaa
     6181 cgtgcaggtc agccaggaga attagcacca gtttatgttt tcttagcatc gaataaagca
     6241 agttatgtta cagcccaaat ttacggagta actggtggag aagcgatcaa cttataagaa
     6301 gttgatcgtt tcacccctat gaggaggtgc gaaaatgaat aaaggtgtaa aaacgatcgg
     6361 agtgatcgga acattacttt tgctatctgt gcttttgttt gcgatactat taagtgcaac
     6421 ctatccattg ccttttcagc tagagaagtt tcgattcttt tccatcacta attcatacgt
     6481 gcaacaatat gtattttggc ttgccgttgc atttgctatt ttgacaatta tcacgttact
     6541 tgttttcctg ttttatccaa aaacaatcgg aaactttcta ctgaaacatg gtgatgggga
     6601 gttgacgttg gataaaaaag caattgaagg aatggttcgt tctcacttgc atgaagatga
     6661 attcatccat tcaccaaaag taaatgtcaa agcaacgaaa aacaagatcg ccgtcaatgt
     6721 aaaaggggag ttgaaacgaa cttcagcact cattggtaaa acaggaacat tgatgaaaga
     6781 aatcgaaaat gaagtacaac acatattggg aacaaaagag ccagtgaaag tatcggtgaa
     6841 atacacaggc tttgaagaac cagctaaacg aagcaatgac cgcaaacgag tagaataggg
     6901 ggggaccaaa aatgaaagaa ctattttcga catacaagct ccctatcatc tttggggtac
     6961 ttggattgat attagccgtt ttatggatca ccgtgggctt tttcaagacc ttattgatct
     7021 tgatcatggc agcgatcggc gtcgctatcg gtttttatct gaaacaaacg aaaatcttag
     7081 atcactattt taaatcttag gaggaaacat ccatgaataa caataacaaa aataataaca
     7141 ataaaatgaa caaccaaaaa ccagtgtctg cacatccaga cccaacgaca ccaaaccaat
     7201 caaatggtgt gaacgatgaa aagaacaaaa cagcaccagt atctgcacat ccagatccag
     7261 tagtccctaa cgttcaacaa ggagtagaag agaagaaaca tgatgcagaa aagcatgcac
     7321 ctcatcatcc aggaactcca gaatctaaag ttccagaaca acatgatgca atcgatcctt
     7381 ctaaacctgt ggaagagcaa aaacatcctt ctgaccatgc gaaagcacaa gaaaaactta
     7441 gttctgacca cacaaacgga caaggaaaac ttggttctga tcatacaaat aaccaaggaa
     7501 agcatgattc tgaacaggaa gattcaatgc gcggtgagct gaattatgaa gataaagttg
     7561 tgcaaaaaat catcggaatc gcaattgaac aagtagacgg tttattatct gcaaacggtg
     7621 gtttcttctc taatgtggca ggaaaacttg tgaatacaga taatgtaacg gctgggatcg
     7681 aaacagaagt cggtaaaaaa caagtagcag ttgatatgga tgtcattgtg gaatatggca
     7741 aagatatcga aaaaatcttt gaagaaatgc aagaagtaat cggcaaagaa gtaaaaaata
     7801 tgacacattt agaagtgatc gaagtcaacg ctaacgtagt ggacatcaaa acaaaagaag
     7861 aatttgaaaa agatcaagaa acggtgcaag acaaagtaac tgacgcagcg aaaaaaaccg
     7921 gtgaatttgc ttcgaatcaa acagataaag taaaatcagc agctggaagc ggcgcacaaa
     7981 aagtaaaaga aaatacagaa ccacgggttc agtaataagg aagtaaaaca ttaggaggaa
     8041 cgaaacatgg gatttatttg gtcacttatc gtcggaggaa ttatcggagc aattgcagga
     8101 gcaatcacga ataaaggttc ttcgatgggg attattgcaa atgtcgtagc aggtttagtt
     8161 ggatcagcac ttggtcaagc attattaggt tcttggggac caagtattgc aggaatggca
     8221 atcgtgccat cattgattgg tgccatcatt ttagtactaa tcgtatcatt ctttgctcga
     8281 aaactgtaaa aagactttta aaaagaggtc gtgaaaaagc tgttcttcat caagtaataa
     8341 gaacagccaa acaaaaatag cttctcatat ttttcatttg gttgggcata ttatcgaaga
     8401 tgcaagcttt tgaacactgt ttatacggaa taagaggctg tgacaagact tttgtcacag
     8461 cctcttattt atgcgggcaa attcccaatt agatttttaa aagtttttcc ttaaatgtgt
     8521 ctttattgat acagagaagc ttctttaata aaacaatgcc tccctaaatt ttcttgagat
     8581 aggaaaggtg aacatacaaa ataatacgac cactttgaaa acccagatga tccacaaatg
     8641 gatcgtatga tactgatgaa gtaaaaagca atggatcaga aactcaaaga gattgaaaaa
     8701 gacaaggaag aaataatatc tcttttttac tcttgtctgt tttagccaat aatagctcgt
     8761 aaagtaatct agacaaacaa atagaagtaa acaaagtttc gtaagagcag caatcgtatc
     8821 atgttgctct gctagacgat tgacgagcag gaaaaatgtt ggtaatggaa tatagatcac
     8881 gagtaatgaa agaaaaaagt aaacaataaa tggcatacta ttcctcctaa ttgtgtgagt
     8941 tcgccctcat tttaaaataa cgatatttgt ttgtgaagct ttttcgtgtc gtaacaaatg
     9001 ttatctaatg tatttttaaa taaataatca ttattatcaa aataatgtta aaaataaaaa
     9061 agaattgtcc atcactgtta ttcaaatcat tttatcaaaa ctacatagta aatgttaaaa
     9121 agcaacatga cataatatat agaaggcttt acagtatata tgggagctta ctaaaaaaga
     9181 atggagtaag agttaacaca atgggccatt agctgaagtt ttaagcactt caaggtatga
     9241 aacagaatgg cctgtaatac tacatgatta tttagactgc tcaaactgct atcagtctgg
     9301 tggaatgaat aaagtattat actaaaagca gatccaacta caacgtaaca ggaaaccagt
     9361 tgtatcagga aaagtgatag tacatggaga agaaaaggcc ccctttgtat gagtcagtac
     9421 atatcttatt tatagataaa acggtattca atcgaaataa aaaatc
//
LOCUS       DARBFD010000098         9391 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000098 DARBFD010000000
VERSION     DARBFD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.100"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            94..1248
                     /locus_tag="SCK08_002348"
     CDS             94..1248
                     /locus_tag="SCK08_002348"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEG1202193.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="SCK08_002349"
     CDS             complement(1270..2022)
                     /locus_tag="SCK08_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1202194.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="SCK08_002350"
     CDS             complement(2386..5052)
                     /locus_tag="SCK08_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1202195.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="SCK08_002351"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="SCK08_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1202196.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="SCK08_002352"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="SCK08_002352"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEG1202197.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            8282..8557
                     /locus_tag="SCK08_002353"
     CDS             8282..8557
                     /locus_tag="SCK08_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202198.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            8984..>9391
                     /locus_tag="SCK08_002354"
     CDS             8984..>9391
                     /locus_tag="SCK08_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1202199.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNL"
BASE COUNT         2830 a         1907 c         1635 g         3019 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8161 tcgctattct aataataaat aaccactatt tgatttatat atatttcagt atccttcttg
     8221 ttttcatata accaaaggcg ctatgatttc catatgaaaa aaataaagtc aggtgaaaaa
     8281 aatgaaactt gaggataaaa aagtcatttt gaagaaaatc aaacgactac cagaccaaat
     8341 agatggctat acgatctgtt ctttcaaaaa agacaggagt ataaaagctt atacgcaaaa
     8401 cacagaaaaa ggaataatct atttcctaca tgaagaaggt tacgataccc aaacctttat
     8461 gtttactgaa aaaaaagaat ttcataaaca gatgaaaaaa ctgattgaaa aagaatttcc
     8521 aagaagtcat aaactctatg tgaatcagca attttaactt ctaactgttc tttattaatt
     8581 aaaaatgttc tgtaataaaa taaaacttta taacaactat aacaaaaccg aatgcaaaac
     8641 agaagttcta taagttataa tcatagttgt taggtgattc atctagtaaa aacaccagat
     8701 ttcaaatttc acacaattta aactagatca atcatcaacc ttcttcaaat tgttagaacc
     8761 ctttcccgag gagctgcagc ataagcaaaa aaacaaacag gaagccaaat ggctaagaca
     8821 ttttttctat tagtctagtt gtttctcgtc gcaagtttaa gactttagaa agttatttct
     8881 aatgatgttt gggttgtttt gatcaatgct tatgcacagc tccttaccta ttatttatct
     8941 caacagatct aacaaattaa tggactattt aaggagtttt tttatgctta cgatctattc
     9001 ttctcgcaca catagttacc atctgatggc aaactggctg aaagaccatc aaatgcctta
     9061 cagagaacga attttcacac ctaaacagcc tttaacaagg aaagagatat tttatttatt
     9121 gtcattaaca gaaaacggat ttgacgatct attagctaaa cgctcgattg attataaaaa
     9181 tttgaaagac caaatcgacc aatgtacgac ctcagagctt gtcgatctga tcgtctcgca
     9241 ttttactttg ttacaacgtc ccatcattac tgacggaaag aaattattag tcggattcaa
     9301 tgaagatagc atgcgtcttt ttatccctgt acaatggcgc agaagtcaac tgaaatctgt
     9361 ctataacgat tttacatggg taagcaatct a
//
LOCUS       DARBFD010000099         9387 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000099 DARBFD010000000
VERSION     DARBFD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.101"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..423)
                     /locus_tag="SCK08_002355"
     CDS             complement(1..423)
                     /locus_tag="SCK08_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1202200.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(413..2587)
                     /locus_tag="SCK08_002356"
     CDS             complement(413..2587)
                     /locus_tag="SCK08_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEG1202201.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2603..4945)
                     /locus_tag="SCK08_002357"
     CDS             complement(2603..4945)
                     /locus_tag="SCK08_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202202.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4967..7510)
                     /locus_tag="SCK08_002358"
     CDS             complement(4967..7510)
                     /locus_tag="SCK08_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1202203.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7520..7918)
                     /locus_tag="SCK08_002359"
     CDS             complement(7520..7918)
                     /locus_tag="SCK08_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1202204.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7999..8283)
                     /locus_tag="SCK08_002360"
     CDS             complement(7999..8283)
                     /locus_tag="SCK08_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202205.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8307..9305)
                     /locus_tag="SCK08_002361"
     CDS             complement(8307..9305)
                     /locus_tag="SCK08_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1202206.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2675 a         2006 c         1468 g         3238 t
ORIGIN      
        1 ctaaaaatta ggaagaaatt gattcaatgt cttttcaaaa aagaatgttt ttctctcaat
       61 ttttttgacg tttctatcat gaatcacgag aaaacttggg acacttttaa catggtaacg
      121 ttgtaaaaaa gtttgaagtt cttcttgtct tggttctttt cttgtttcaa ttcgatatac
      181 attcctagag aattttttgt cgtatctttc caagaaagat tcaacttctt ggcatttttt
      241 acaatcattt cttgctagat acacaaaaaa tgattgattc gttttttgaa gttctagtac
      301 ttcttctaaa ttactattga ttagttttcc atttgattct tcgttagttt ttatcagttt
      361 aaaacaacca aataagagga aactaacaat aagtatccta acaataactt tcttatttcg
      421 catattgttt ctccaaattt ggtttccgaa tatagacgac tcgcggtggt tcctggacgt
      481 cttcagctgt tcgatacacc acaaattcgc ctgttctcaa tgctcgaatg cgattcggat
      541 tgaatttaaa gcggtctacg tctcgtttcg tgcctctttc tcctgtccaa tcaacctttc
      601 ccatatcaga ataacctgct tcttgtgtcg tcatttccgt taattcaatg tcttcttctg
      661 tccccattaa atctgcccaa taatcaattt ctgtcgggtc atttgtcttc ccaataaaga
      721 acgtattcac attcccaatc aactgttttc gaaggacagg atcaattcta tcaatgtccg
      781 caagcgtttg aggggcatac gccgcatgca ccccagcgcc tcgtccctta ttgactaagt
      841 ctataaggtt atcatttaga taagaagctg gttcgtcaaa tattactagg actttcgagc
      901 gattggctgt ttctcctgct tgtttataca cttctgacgc aaagaaatta atatctccca
      961 caagcatttg agccaatgtc cgaataaact cactataaat caatccgttt aacgagatat
     1021 aaacaatctt tccttcttta acaacttctt gaatatctaa ttctcttttt cctccagaag
     1081 tatcaaagag ttcaccaagc tcactatcca ataacatatt gatattggtc tgtaaattct
     1141 cagaagtcgc atacaaataa aacgggttct tcttatgttc gtaacgaaca aacaaccgtt
     1201 taaacaacgt ttgctcgatt tctgtcatga gagaaatgtt ttcttttaat aatagataat
     1261 acgtttctaa gtcaacggtt ttacgatcat gttcaacgat tcgctcttca cttaactgtt
     1321 ccacatcggc aaaatcacgt tctgttttgt ccatcgcttc attctttttc gtttttttct
     1381 tcgtggtttt cttcttttct acgaccactt tttctttaaa taaatctgga tttttcaact
     1441 gaatacgatc cgcaaacaaa tgtaagacgt ttcgtggcaa taaataatgt tgtaaccaaa
     1501 ctagatttct ttcaatttta tggctctttt ggaactcgtc taaaagctga atcgttacct
     1561 gtaataacgc tttggctgct gtcgaataat acacgctttc tgtttctgct agattggtta
     1621 atttatctcg aatggcaata tcattgccat tttttacagg attataacgc aaatcgccac
     1681 cgtccacaaa gacttcaatc gggcgattgt atgcctctgc cacttctttt aacgcttgta
     1741 aagtctctcc tgctcctttc ccatcgacta agataacggg gatacgaaac ttacacgcat
     1801 gttctgctag taattggatc accgtcgttt ttcctgaacc tgtagtcgct ggaataatca
     1861 catgttggtt caattcacta aagttcatca cataagggag cccatactca tctgttccta
     1921 atagaatcac tttggctaat gaagccagtt gtttattccg caaagcaacg tcttttatga
     1981 caattgctac ctggtacgct tgctgatact ctgctgtata tttttgggac tcctgaaatt
     2041 tattcttccg acgttccttg tattctttac tatttagcaa agctgtttgc cgttctttct
     2101 ttgaaacaac ttttcttttt tcaataaaag acgcatacgc aatcaaacaa cttcctgcac
     2161 ctgtcccacc taaaaggagg gcaaaaatcg tttgtccatt aaactgcaac gcatgagaaa
     2221 acaagtttaa tggaaataat aagtaacacg acaaataccc tctaggaaga acaaaaataa
     2281 aacaaaatag atttaatagc gtaaaaccta acgcaaaaat aaaaaaaaga gagcttcttc
     2341 gttttaagaa gctctcatac tcaaagtcat aaatatgtgc acgtgtctct tttttatcta
     2401 tcataaagac aatcgctaaa ggaatactgc ctacaataaa aaatggatat agacaaacac
     2461 ctaataaagt gacgataaaa taaagttgtt gaaaccgact ttttggcgtt tctttttctt
     2521 gttttctttt gcgatcgaca gaaaacattt gctcaaaatg tgtcggtcgt tttggtgcat
     2581 tcatcatact atcgatttcc tcctatcgtt ttcgttgttt catttctctt cgataaatcg
     2641 gttgtttttc tcgttttcgt ttcatttttg cttgagttgc tcgaaactca ctccgtgtct
     2701 tcggtgcttg ttttggtgat tttgtttgtt tcatggttga gtatgcttta gatacggttg
     2761 aaggcgcttt ggtttgtgct gttactcgat tgactgctga catatgttcc cgaaccccct
     2821 ctggacgtcg aacagatttc ggtgtggtag aacctgttgc tcgtggtaca ttcccttttc
     2881 ttagtggttt ttgtaccgca tgtttttgtc ctgcaactgt ccgggttttc atcgaacttg
     2941 tttttgttac gggagtaagt gcctccttcc gacgacgact tgtttgtccc tgacgacgtt
     3001 ttcgatcgtt caattctgtt ctaggcgtat gcttctggcc ataagacttg tgtttttgct
     3061 ttggattcgc taatgacacc accattgcag aaccctgatt gcgatacgga ctacgagacg
     3121 caccttttct acttctttct cttagactag gattgccttg caattctcgt tgtaataaat
     3181 cggctgtttt tcttgcctgt ttctccattg ctgttcgttg catacgttct tgactttgct
     3241 cttctgcttg tttcgcttga tcatgcaagt ctcttgcttt ttgtgccata cgttccatac
     3301 gatttggtgc tttatgacga ggttgttgtg cggattcacg ccgaaaagca cttcgtgtgg
     3361 cttgtttcgc cgtttgtggc atacgaacgt ctgagccacc cccacgtttt cgttgacgtt
     3421 cttgtctctt ccgtgcgttt tctttttctt tttgtttctg tgcttttctc gcttctttgc
     3481 gttctttcaa ccgttgtttg gcttcccttc tcaattttgc ttctgcccca aatggatttc
     3541 ccgcaaaagc taacgctcct cgaacaccca ttcttccagt aaatagactg cctaaaaagc
     3601 ctcggaacaa ataaattccc aacggcgcaa acaaataaac aatgatcaag agaatttttt
     3661 cccatggatc gtcttgtgta ctacttaatt ggaacccgta actaagaaaa gaagaaaaaa
     3721 ggatacatgc aaaacctgtc attcctttta aaaacgtcaa aataccaaaa actttggcat
     3781 agttaccaaa cacattcatt gtgacaagaa acaatcctaa aaaaagtaag aacggcaaca
     3841 tcggtactaa caatagttgc atagttccaa taatcaatcg aaacatactt aagacaaaat
     3901 aaacaacggt ttgaatcaga ttcacgacaa aataaaacaa ggcaaatgct gtatttttcc
     3961 aactattgta atattgtatg ttcttgtttt tcagtgtatc tgcttcatac tctgtgactt
     4021 tgtcatgaat tttctcccct atttttgtac ctaaatcatt gtccaataaa agattgattc
     4081 gatcatattc ttttccagca tatttgactt tcttctcacg aattgtgtct acattggttg
     4141 tgccataatt taacaacaga tatggttcat aaacattact ataaaagacc cgagccgcca
     4201 tcaatttgcc tgccgtttcc acttttttat ccgctgttac ctcttctgtt tcttttgttt
     4261 cataatttgt ttcttcttca ggtgttaaca ctggattgac cttcacgaac gccgtttcca
     4321 ctgtagaatc taaagaaaaa agaacatcaa aaagattctt atttgtccca gaatctttta
     4381 atacgcttaa cccagtaaaa ataaggatcg tcataagaaa gacacggagt gcttgtttca
     4441 ttcgagcctc tccagcatat ttgattgcca tcataattgc cgaaaaactg attccaatag
     4501 ttcctgcaat acttaacata ttgtccgcaa ttttttgtgt taagttttga atgggttctc
     4561 gaatggcgga aataatatct aattcaaaga gaaattcaac cattgaagca ttgaattctc
     4621 ctaacatttt tacgccactc caaacaaaat ttttcatcac tcctgcaaca ttgtacgata
     4681 gctttttttt aaggttcatt gactcatagt cattggtcat caattcaaac gaattctttt
     4741 tgtaattttt atacaattga tccacatcag tcgttacttc ttttacttct actttatcgt
     4801 tggcattctc tccttcagaa cgatcggttt cagtgacacc cgcaccatca ttcatttgat
     4861 cggcagaaac agtagcccct tgtagacaga aaaaaagacc ccctaataaa aggagcgtcc
     4921 accatttcag agattttttt cgcaaaaaaa ccacctcttt tttgttttat cgttgttttt
     4981 cattttctaa cattgcttga attttttcat cttgattttc agtagaagaa acagccaata
     5041 accaactatc aaacaacaca tcaatggcaa tcggttgatt tcttcccaaa tgatcctgga
     5101 ataaacaggt tcctgtttgt aattgttcca tcatttctaa attggctgaa ttcacttcca
     5161 tatcaaagaa ggacaatagt ttctctcgtg cttctttttg tttcggacga aacgcaaatt
     5221 tgtaagaaat taactctttt ttatcttctt gatcgtaatc tttaaacgct tgcacaacaa
     5281 ggtaaatatc cgtattattc gcacgtcctt ttcgcaagct atcatcaatc aaatagcttc
     5341 cttgtgcggt atcttcatac ccttttgcct cgtcataaat aatcgcagcg tcttcttctt
     5401 tgtttgtaga aaagatatac gtaaacttca taatgacttc cataattgcc attcctgcaa
     5461 gttgttcgct atttaatcga cccgaatcaa tctctttttg tgtcggaatg cgtattcctt
     5521 gtgtccctaa cacgttgatt tgtgaatcaa aacgtatcgg cgtactgtgc tcattcccaa
     5581 tgagaatctt tcctaaacca acgctatgcc ctaaaatcaa tttagctagt tctttgtcac
     5641 tttttcgaat ttcttcgata acttgactta acgaatgctt acctggcatg gtgtaaacta
     5701 catcgatggc gtcttggatc accgttttct tgtggctcgc tgtttctcga ttggtatcta
     5761 cttctcctaa cattcctaac acatttttag cggtttgaat tgcctcttct tttggtaaaa
     5821 acaatagagg atcaagcatc cctcgacagc tttcatctga agataacgaa ataaaattaa
     5881 ttcgttcata catagccaca aactctggta tgtgtccgaa tttttcaagt gcctttaaaa
     5941 agaacttttg ggtttcattt ttcggatcaa tataaagaat tttttgacct aaaaacatca
     6001 gccataaaaa aatatatttt acaaggaccg attttccttt acctggtgga ccaataatca
     6061 acgtatttcc attggtatgt tccgcgcctt caatactttt ctttgttaaa ttgggataat
     6121 accatactag tttactagat agcgctaacg cttctttcgc agatttaaac ttttttgcaa
     6181 taacaatccg tcctaaagga aatcctttct tatttcctac ttctttcttt aagtccaagc
     6241 cataatctgc cacaaaacct gtcgtagcga cctgctcgta actacgaaaa cgttgcgtac
     6301 ttccaattaa gcattgatta aacaacgtta gctgatccgc taccgatcga taaaatttga
     6361 aatctgtaga ctcaatcgca aattcaatat ctttgactcg ttcttccaat tcttcttttg
     6421 aagaagcaga tacaaccagc gtgacggtag aataacacaa tcgtttttct ttgcgcttca
     6481 gcttatcttt tacatccatt aaccgttctg taccaaaatt taatacatcg tctgaatcta
     6541 aaatgccgtc aacggtctca tattcttttt cttgttcata aatccgtcca gccattttat
     6601 taatttttcg ttcatcagat tttgaatgct caaatcgaag tcgaatatgc gtatcaattg
     6661 gaaaagataa gctatcctga agattttgaa taaagctact cccatacatg gctaaaggag
     6721 cgtccgtttg acttaaaaac gtcgaataat gcgtttgatt caactgttca atcgcaatgt
     6781 atcctgcttc attcgaaatg acgccctcgc aaatttccat tggcgtaagc tgtctttcag
     6841 gcaatttttc ttctgatcga tggaaaaaat aataataaat ttgtccaatc tcttgggcag
     6901 ataagcgctc tatcggctta taattggaca attccgtata gagctccttt tctaatgttt
     6961 catacgttgg taacacacta cttataggaa ctcgttgtcc cgtaatacga tagataaact
     7021 ttttaaaaac atgcttaagc aattgaaaat attcgatcgg atcagacact ttcatttctg
     7081 tagtgaaacg cacaaacaga tacgttcgat actcgtttaa ttgcacttcg tcttttaaaa
     7141 tctttcctgc ctgttcaaag tacactttcc caagatctgc gcattctccc tttaccaaat
     7201 ctcgaatggt ttcttcaatg tgcgtatcta ggttaaattg acgtggaata ttgataaaat
     7261 gatagtcata ttcatcatgt ccaagtatgc cttcgccatc ttgaagatag ttcttgaaaa
     7321 aatcaatgtc atttaaagga aatacttggg aagaaagttg atagcctacc cacacatcat
     7381 catttttggt caaaacaaaa tttcggataa tggcgtctat cggaaattct aatcgaggaa
     7441 gcttcgtttt tttctttttg aactgccaaa tcccgataat aaagacgacc aaaagaaaca
     7501 aaaacgccat ttactcgact cactccttcc atttgtgtag ggaatacgtc actttttcat
     7561 ggaaaaacgg cacatacatt tcatgttcaa agcgttgttt ccgtttctga taataggacg
     7621 ttcgcccaat taaatactcg aaaaagtgtt tattgtccca tttcattgaa aacaatgtcc
     7681 aaacgagaac accaatccca aaaatcataa tcaaccagga ctttcgaaac agtgtctgca
     7741 agaaactaat ttgattcaca tacgcaatca aaaataggat caaaagaatg aagagaaata
     7801 ccccacctat gactaattgt tccatgcgaa tgccattaga aatccaggga attttttgtc
     7861 ctttaatccc gtaaattttg tgagggtgct taaactcctt tgtatagttg tattccatgt
     7921 tcactctacc tccttttaca aaaaaagaag cgaccagaag ttctaatctt ctgatcgctt
     7981 ttgcagtttg tctttttctt atgataattg tatccagggc acaatcattt gcgttacccc
     8041 cacgttctct tcactatcct taataaagat aatgttcttt agccattcac caatatcaac
     8101 ggcattcgat aacaataaga cgagcattat caaaataaag acgccaataa tggcaaacgc
     8161 tgcccgcgca aagccttggg tcgcataagc accaaccatt agaacaatcg caacgattgc
     8221 caatagccaa atgacttgtc cagatacatc atcaaataac gatttaagat caaaaagttt
     8281 catttccatt ctcctctaag ttcattttat tcatttaacg tagtaacaaa ataactatcg
     8341 ccacttgttt ttacttccat tgaaaagttt gattggaact cgtttccttt ctcaaaatag
     8401 aaacgatact tcccttgcaa aaagtagttt ccttctttat tttttactgc atgatccagt
     8461 tgtacattga tcaattcgcc gccttctaac cctttttcgt tagaaatcaa tgccaatttt
     8521 tcatcatttt tgacatataa ctcaaaaaac cgtttcacaa atgcttccag cttgatttct
     8581 tcgctttcag tgaccgcttg cccagaagaa taaaatcgat tttcctgata gttcacagaa
     8641 ttggtattct tactctcaga aagattcaaa aattgaacgc catcaagtac ttttaaaaca
     8701 ttgttttgat aactgactgg aaaagaaagt tgactgcttt tccaatgttc tccttctgta
     8761 aaagtaacct cataaaatag gcgatacatt gcttgtgtca aatcgatttc ttctagttta
     8821 ataaagcgaa catctttcgc tactcgattg gtagccgtcg ttacaaagcc tagatcagac
     8881 gcttccaatc cttgtgctaa ataatgattg acggaagcct cccattcgtt tcgtttttct
     8941 ggcgaataag taaataacct ttcaataaaa tgttgtccct cgtacttcaa ttgttccgta
     9001 gaagacaagg tttggttgac ttcagttaac aatgtttctg gattgacttg ttttttcact
     9061 gctaaacgcg ttaatgtatc cactcgtcca aagtttagga ggagcacgac acccattaac
     9121 agaaacaaac aaaaaaaagc gcctgaaaat acactcatgg cacttttttg cgaaaattgg
     9181 atcattcgat ctgacggttc tgttttcttt ttttggaaca aaaaacgttt cttttgtttc
     9241 ttttccttct gttgtttcgg tggcttagtt tggaacgctt gtggattaaa ttttgcatta
     9301 ctcatgagaa atgccatacc gttctaacaa taatttcggt attttgtcca caatcactac
     9361 ttcgtggaca accacacttg ccccctc
//
LOCUS       DARBFD010000100         9358 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000100 DARBFD010000000
VERSION     DARBFD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.102"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCK08_002362"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCK08_002362"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEG1202207.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCK08_002363"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCK08_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEG1202208.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCK08_002364"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCK08_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEG1202209.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCK08_002365"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCK08_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEG1202210.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCK08_002366"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCK08_002366"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEG1202211.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCK08_002367"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCK08_002367"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEG1202212.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="SCK08_002368"
     CDS             complement(8053..8523)
                     /locus_tag="SCK08_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEG1202213.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="SCK08_002369"
     CDS             complement(8569..8994)
                     /locus_tag="SCK08_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEG1202214.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="SCK08_002370"
     CDS             complement(9061..9330)
                     /locus_tag="SCK08_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEG1202215.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
BASE COUNT         2355 a         2095 c         1621 g         3287 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta attttttt
//
LOCUS       DARBFD010000101         9278 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000101 DARBFD010000000
VERSION     DARBFD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.103"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..435
                     /locus_tag="SCK08_002371"
     CDS             <1..435
                     /locus_tag="SCK08_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG1202216.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="SCK08_002372"
     tRNA            complement(759..842)
                     /locus_tag="SCK08_002372"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="SCK08_002373"
     CDS             1370..1870
                     /locus_tag="SCK08_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1202217.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="SCK08_002374"
     CDS             complement(2060..2404)
                     /locus_tag="SCK08_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEG1202218.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="SCK08_002375"
     CDS             complement(2558..3478)
                     /locus_tag="SCK08_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1202219.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="SCK08_002376"
     CDS             3665..4156
                     /locus_tag="SCK08_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEG1202220.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="SCK08_002377"
     CDS             complement(4180..6555)
                     /locus_tag="SCK08_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEG1202221.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="SCK08_002378"
     CDS             6881..7507
                     /locus_tag="SCK08_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1202222.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="SCK08_002379"
     CDS             7530..8234
                     /locus_tag="SCK08_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1202223.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="SCK08_002380"
     CDS             8426..9259
                     /locus_tag="SCK08_002380"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEG1202224.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1790 c         1651 g         3009 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttct
//
LOCUS       DARBFD010000102         9266 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000102 DARBFD010000000
VERSION     DARBFD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.104"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            135..1349
                     /locus_tag="SCK08_002381"
                     /pseudo
     CDS             135..1349
                     /locus_tag="SCK08_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1528..1857)
                     /locus_tag="SCK08_002382"
     CDS             complement(1528..1857)
                     /locus_tag="SCK08_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1202225.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1847..2119)
                     /locus_tag="SCK08_002383"
     CDS             complement(1847..2119)
                     /locus_tag="SCK08_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1202226.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2570..3373)
                     /locus_tag="SCK08_002384"
     CDS             complement(2570..3373)
                     /locus_tag="SCK08_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1202227.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3351..4475)
                     /locus_tag="SCK08_002385"
     CDS             complement(3351..4475)
                     /locus_tag="SCK08_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1202228.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4491..5306)
                     /locus_tag="SCK08_002386"
     CDS             complement(4491..5306)
                     /locus_tag="SCK08_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1202229.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5296..6168)
                     /locus_tag="SCK08_002387"
     CDS             complement(5296..6168)
                     /locus_tag="SCK08_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1202230.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6253..7542)
                     /locus_tag="SCK08_002388"
     CDS             complement(6253..7542)
                     /locus_tag="SCK08_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1202231.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7770..8414)
                     /locus_tag="SCK08_002389"
                     /pseudo
     CDS             complement(7770..8414)
                     /locus_tag="SCK08_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(8891..>9266)
                     /locus_tag="SCK08_002390"
     CDS             complement(8891..>9266)
                     /locus_tag="SCK08_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1202232.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2860 a         1977 c         1479 g         2950 t
ORIGIN      
        1 gagttgaaac gaagaaaaag tgttagtaga aaatcgcctt cgattttcca ccaacacaaa
       61 atattataga agcaaaatca attctacctt agatgttctt atatcactct atcagtccta
      121 tttgacaaag agccaagaaa aagacgttgg tcgtcgatgc tgttctctcg cctactcctc
      181 gtgcttgtag aagttgtggt tctactgtgg tagatggaaa cgggaaagca attatagtga
      241 aaaatgggaa gaaggaaacg attgtccgtt ttgaacaata caatcatatg cctttggtta
      301 cgcgcctaaa aaagcaacgc tatacctgta aaaactgtcg tacccattgg acggctcaaa
      361 gttattttgt ccgactcagc cattcaatcg cagatcatgt tcgatataaa attgcttctt
      421 tactgactga aaaagtatct ttgtctttta ttgcgaaaag ttgtcaggta tctttgacta
      481 ccgttactcg tacattgaaa gagtttaaaa gctatttacc aaagccttct aagaagattc
      541 tccccagagt attgatggtt gatgaatttc gttcgcacgc ttccatagaa gataaaatga
      601 gctttatttg cgcagatggt gaaacaggaa acttaattgc tgtcttgcct acgcgtaaat
      661 tacctcgatt aacaagctat ttcttaggct gcaccaatcc agaagaagta gaattcttag
      721 tcacagacat gaacgccgct tacttccagc ttaccaaacg tgttctgcca aatgcgaaag
      781 tagtgattga tcggtttcat attgtcaaac acatgaatca ggcactcaat gagttgcgta
      841 tccgtgaaat gaatgagctt cgtaaagcag gacagaaaag tcaggcagaa aaactgaaaa
      901 agaactggcg ctttctgcta aaaaatcgtg caagcatcaa ccattatgag tacaaaacat
      961 ggaaaagttt ccgagcacca aaatacccat ttctcactga agcaatgatg attgatcgat
     1021 tgcttgagtt ttctgcgcct ctaaaggagg cgtatccctt ttttcatgaa ttagtcgaag
     1081 cctttcgaga caaagaccct gacttatttt tctccttatt ggcagaactt cccgaaacgt
     1141 tagatgacgg ctttcgggag aagcttcaaa accttctgac ctatgaagaa ggtatcacca
     1201 gcgcaatgat ctatccttat tccaatggaa aaattgaagc gaagaatacc cacataaaga
     1261 caatgaaacg agtatcctac ggatttaaat cattcgagaa catgagaatt agaatctttt
     1321 tgatcaatca attaatcaat gtaagataac aaaaaatctg aaccagaatg tggttcattc
     1381 tgacccggat ttgattttgc acaactcatc agtccttatt gacaaagagc caaaaatctg
     1441 agccagaatg aggttcattc tgacccagat ttgattttgt acaacccatc agtccttatt
     1501 gacaaagagc ccttcttttt agtattatta taattcttcc atgcgttctt ccagtcgctg
     1561 taaatcgctt ggacttaatt ccccaatttt tttccatttg acctttttgt tgtttttggg
     1621 taattcgttt agagttccta cgtcaatcca agattctttt tttagtcctg cctgtttcca
     1681 atcccttatt tcaacgtatt tcagctttat tttatcggat ttagtatgat attttgaagt
     1741 aattgacaga aacgaaattg tgtccagtcc attttttaga atatagacag gccggcgttt
     1801 accgccctct gcagctgtga aagcaacgta gacggttgct atatcattag tattcatctt
     1861 cattgaacca ttcttcaatt ttttcgtcag tgtctagttt ttggacaggc aatttttccg
     1921 ctaatctttg aatattatgg attcgtttac ttttttccga caattcaata gaaaatggga
     1981 tttttccttg ctcaacaatt tgcttataaa aaacggtgac ggctgttttg gggctcatgc
     2041 ctagttcatc aataatgctt tctgcttcat ttttcaattt ttcgtccata gaaatttgta
     2101 acttagccat tttctccacc tctttctgac aaacacatta tatcacggaa agagttcaaa
     2161 tggaattaat acaactactt ttgaactcct tgaaatgcag tgtatttatc taaatataag
     2221 gaaaggatat agtgctaaat ggccttgata gatagaaaag tttcaaaatc cgccacagcg
     2281 ataattgaag cgaacactca acgacacaca gaacaggaaa agactcctaa aagaaccaaa
     2341 ttaactagga cgtgtctgaa aactccacaa agatgtggta tgctctggta aaaaacgaat
     2401 ggcacaacca cgaagaccga tatggttctt ggaaaacttt ctccaccctt tttgtaattg
     2461 gagggatagt ggtttattga tagcgatttt ccaagttctt caagtggaac cagacattga
     2521 gcaccacggc taaaaagtga atgaagtctt tatttttaat caataatttt taatgattac
     2581 cagtaattgg ttttttcatg cgccctattt ctatttgatg tggagttaaa taatgctgat
     2641 aagtaaaaat ttttccgtca attaaactgc cactagtgaa tcctgttcta ttggtaaaaa
     2701 gagaatagtc tgggtattca tctacaccaa atgataaaga ttcagccata aactgttttt
     2761 taccagcaaa ttcagcactg ctaccttgat ggatatgtcc tacaaaaata cctaaaatat
     2821 cactttcggc aatcaattca gcaaaacctt ccggaacttc agttacaata gatggatctt
     2881 cccacgacaa tggatgatga accaataaaa tcgttccctt ttcagcaggt gtacccaacc
     2941 actcttgtaa ttgaatcatc tgtcctatcg atatttttcc ttcgtgctgg gtatcttgag
     3001 cagaatccaa ggaaataatt cgtaacccat tttgattcgt acaatggata tactctcccg
     3061 tttcattgct ttcaacactc attccttcaa agaaaacact gcgacgatca tgattcccac
     3121 gacaaaaata gtaaggtgtg gttgggaaat attgatccaa cattttccgt aagacttgat
     3181 aatcagaaat ccccccttca tgtactaaat cacctgttaa tagaataaaa tcgtacactg
     3241 ttaacgagag ttcttgtaac aatttttcca atgccaactg tggattttta attgttggaa
     3301 atagttcagc agtaaacaag ttagtttgat agttttctct aaaatggata tcagagagat
     3361 gcaaaaagcg catggctatt ccctctttct tcaataaaat aatcaggata tcctaaattt
     3421 tcttgcgtct gataatcaaa aaaatgcatt ctttcaggtt ctatgcggag attgactgca
     3481 tcaccaatct gccaagtagg tttttcatta aggacaatca tttctatacc atcgacttta
     3541 acggtgtacg cataactttg acctaatagc tcacgatact tcacttgacc gtgtaaatcg
     3601 cttgtttctt ttgaaaaggt tacgtgttct ggtcttatgc ctaaatacaa tgagccacta
     3661 acctgaagaa gttgtttttg ccaagctgga ttcaatgtta aaaattgact ggctaccttt
     3721 aatgttcctt gatcataagt aactggcata atattcatgc ctggcgaacc aataaaattt
     3781 gcagtaaaag tatttttagg tttcgtataa acatttttcg gcgtatcaat catctgaatt
     3841 ctaccttcat tcaataaaac aatccggtca gctaacgtca tcgcttcaat ttgatcatga
     3901 gtgacgtaga caaaagtttg tttataacgt tgatgaattt ttaccaattc ttttcgtgca
     3961 tccaatcgta aacgcacatc caaatttgat aacggttcat ctaataaaaa ataattactc
     4021 cgtttaacca ccgcacgagc caatgctaca cgctgccgtt gccctcctga caaatcttta
     4081 ggaagtcgat cttggtatgg cgttaaacct aacatcttca aagcttcttc caaacggtgc
     4141 tgaatgtcag ctgtcttaat tttattcgcc cttaatgcaa aaataatatt ctcctttaca
     4201 gtcatgtgag gatataaagc ataattttga aaaaccattg cgatctctcg ttctccacaa
     4261 ggtagcttat ttgctagttg tgtacctaaa aaaagctctc cactcgtaat tttttccaaa
     4321 ccagcaatca ttctcagcaa agtagatttt cctgaaccag atggtcccag taacacaatt
     4381 cgttctccag cggccacagc taaatcaatt tcttttaaaa tggtatgttc tccatatttt
     4441 tttccgatat ttcttaattc aagcgtttct cccatgattt gtcctccaat ttatttaata
     4501 cctgcagaaa caaaagtccc gataatatat ttttgaaaaa ttgcatacaa gactaacggt
     4561 acaaacattg taattaaact aatcgccatt gctacggtaa aatttgtccc accttcagaa
     4621 ctaatatagg tctgcatcgc caacggcaaa gtataattat ctactgtccg taaaattaat
     4681 accggccaaa catactcatt ccaagaggta ataaaaaacc aaataccggt agaagtaatc
     4741 gcatgtctag taagaggaaa aacaatttta ctcatgacgg ttgtttgttt gatgttatcc
     4801 aatgccgcca catctagaag gctcactgga atttcccgca ttgtttgcgt caataaaaaa
     4861 atgcccatcg catcagcaaa ttgtggtatt atcacacccc aagtagtatc aattaaatta
     4921 agttttgcaa tcaacaaata attaggaatc atcgcaacag taaaaggaat gaacatcgta
     4981 ccaatgatga taaagtacca gattttcttc cctttgattc ggcaataagt aaaactatat
     5041 gccgcaaaaa aagctacgcc taacttaaca accgttataa ttgtagcaat aaaaaaagta
     5101 tttctcgtaa ttttgataat tggaagattt tcgaacaaat agcaaaaatt ttccaaggta
     5161 aacggctgtg gtatcaaact agtaatcgtc tgataggcat ctttcaatgg cttaaaagaa
     5221 ttggccataa taaacaataa tggatagata ataatgatta tgatgaacat aaataagaga
     5281 tgtggcaacc agcgtttaat tttcatagta aacccccttt tccacaaatt taaatattac
     5341 ccctaagaaa acagcaaata ttaataaagt taaaactgaa attgctgaag attctccgac
     5401 tttatacatt tcaaaagcct tttgataagc ataatagatc acgtttgcac taccattatt
     5461 cggtccacca cgagtgatga tcttaatcgg agtaaacaca tactgcagac cttgaacaat
     5521 ggtcatcacc aataaatata gtcctgtcga actactcata ggcaacacaa ttgtaaaaaa
     5581 aatgcgccag atgggtacac gatccaactt agctgcttcg atcatctctt gatcaattcc
     5641 cacaattgct gcaaaaatga caatgaaatt ataaccaaaa actttccaag cagtaatacc
     5701 tgcaatcaca gcaattaccc aaccttgtaa attcatccac ataggcagtc ttaaaccaaa
     5761 atttcccaac aagacagcaa ttggtcccga taccggattt aatatccatg taaaaaccat
     5821 ccccgccact accaaagaaa tcacagctgg aataaaaaat aatggtttat acaaattctg
     5881 ccctttttct ataactattc ctaataaaaa ggatgtaata tatgggaaca cacagctcag
     5941 caagacaaat agaacggtat aggacaaagt attcttcaac actttatgaa agactggatc
     6001 agtcagcacc tttacatagt tggcaaagcc aacaaattct ttagtggggg ccaccatatt
     6061 ccagtcaaaa aaactcaaat agatcgtata aatcaatgga taaaaaacaa acacgcccaa
     6121 caaaataatt gctggcgcca gatacaaata acctacatag cgcttcattt ccgacagcct
     6181 cctcatcctt atgtcaaaag cagacgacct tgtttttcct ttcttacagg cgctgtcgtc
     6241 tgcttgcttt gcttatttga gtaactcatt aatttccttt tgagtagctt tcatcgtttc
     6301 attgacggaa ttacctgata aaattttatc ccgcatatcc aacaattttt gttcagcttc
     6361 taatccagca tctccaggaa atgaactcca actgaccatc ttatccatct gatcaattgc
     6421 tggtatcatc atctgatttt cagctaagaa agcttttaat ccattttggg cgtctgcaac
     6481 atctttacgt ggtggtaaat aaccagtacc ttctgtccat ttagctaaag attcaacact
     6541 ataaagatat tttaagaaat cccaagttgc ttcctttttc ttatccgttt gtgatgtaac
     6601 agctaacatt gcgccaccag caggtagttt aacttttttc ccttcccaac ttggtgactc
     6661 aactgcttta gcagtaaaag tactattttt ttgaatgttg tccctttgag caatagtggt
     6721 atagaccatc gcgacgttac catcaataaa gctttgcaca ccttgttccc acgtagtgtg
     6781 taatgccgtc tggtctttta ccatcctttg atataattca taagcagcga taccatcttt
     6841 agaagcaaag gtagctttgc catcttttat tacacttgcc ccattacttt ctaaaattgc
     6901 ttgttgtgcc caattatcag caggttcttg aatatacacg ccatactttc cagttttttc
     6961 ttttattatc ttagaaaaag tttgaacttc ttcccaagtt tttggaccat tttcatccaa
     7021 gcccgctgct tttaatatat cttgatttaa atataaaacc ggattactaa tggagtaagg
     7081 aatacccact tgtttaccat ctttttcaga cagtgccatg atattgtcta aaaaatgatc
     7141 tttaaaataa tcagcattat ccggatccac ttcttctact aaagattgag gattagtata
     7201 tttgaaattt tctgcaaaat aatctttaaa agcccatccc acttgtacta cgtccggtat
     7261 ttctttggag gcaacagaag cttgtaaatt ttgcatcaag cctttataca tatcaggatt
     7321 atattttgct acaaccttca ctttgtcttg agattgattg tatttatcta ctaattcatt
     7381 cactgttttt ccacctaggg tatccgcatt aacatgccaa tactctatcg ttacacgacc
     7441 ctccgatttt tttgttgtct tggtattccc actaccacaa gctgccaaag agatgacact
     7501 taaaacagac acaaccccta taacaagatt tttctttaac ataacattaa cttcctccta
     7561 atttctttat ggccattttt ctaatcaaga aatattatga actaccaatg taaatttcta
     7621 gttaattttc tgtaaataat tcgaaatttg tgcatttcgg ggtacagggt cactggacat
     7681 atccatcaag ctaagaataa agcccccata tggtcatgta tcccatctcc aaatctctta
     7741 actcctctgt tttgattata aaatagttta ttctgtgctc ctttcttcca taaataggcc
     7801 catgcacaac gtgaggttat attataaaca ccacagcgcg caaggtactc aatcgtttct
     7861 agttcattga ctgtccatac agaaaatgtc aatcctagct gctgccccac ttcacgggcc
     7921 aacttagtta accatccttc atgaatattg acgatttgaa cgctttgata ggctaatttc
     7981 tgtaaggtat cgatcaaatc tgcttcggta atatccccaa gttttttccc ttcgaactga
     8041 ttttcaatat tataaaatat atgctttccc caaatcgcag cccaagataa ttgtacatag
     8101 ccactaagaa caatttgtgg ccagatttga taggtttgtg ccagcgaaaa aacggccatt
     8161 tccaatccct cttcttttaa atcacagtta atggtcatgg ttgccttctc attggccact
     8221 gcttgaaaca cctgctcaag ggttaccgaa tctgggacaa cttcttacct gtcatgggct
     8281 agtattagcg taccatctgt ttgctgacgc acatctactt caaaacaaag ctggcgatcc
     8341 aaacaaaagc gaagaaatgc ccagctattc tcctccgtat tgtcactacc agagtgtgca
     8401 gtgatcatgg tcattttgac ccctccttta attccgtgat tttctcccac acgtccacta
     8461 taccgatgaa gcgttaattt cttgttaatt tcttgtaaat tccctgcgtt ttttcagtaa
     8521 actagttgta aatcttgcat gctccttcct acgaactcat gaatcgtcga ttttgccctt
     8581 ctagctagtt tcaccaatta agtagttcta cattccccgc ttttaaccta ctacaccttt
     8641 ttttgttttc agctattcct tcttcttgct gaaagtcctt gaggttcttt cttacaggat
     8701 accatcaagg tgttcaatgg cttattccca ttgaatgacc gagacttact atcagatttc
     8761 tgctcgttga accaagtctt tgatacagga tttcttccgt catcttctca caaataccac
     8821 cttgcgtttc tctagcgatt ggtcgatgtg caccctccca gtggactgtc actacccctt
     8881 tttgctatgc ctagcccaca aaaaaagctg tcctcacaga cagccagact gtagacaaaa
     8941 gctgttccaa actcgtaact cgagcttgga acagcttttt atctacttcg tttcgaattt
     9001 ttgaacaaaa aaataaaggc tccttgcctt tccatagcca ggtggctaat ttctttaaat
     9061 tcattgcagc aaacactagc atcgtatgcg tcgcgacttt ttccaagcct cgatacttgg
     9121 tccaacgcat cccatgcttt tcttttgcat ccgcaaatat tcgttcaatc gtttgtttcc
     9181 gttttttgta aattgattta ttcagatctg ttaggcgaag atgttccact tcatccatca
     9241 gatctcccca aacatgtctc gtgatg
//
LOCUS       DARBFD010000103         9048 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000103 DARBFD010000000
VERSION     DARBFD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.105"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="SCK08_002391"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="SCK08_002391"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEG1202233.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="SCK08_002392"
     CDS             2714..2938
                     /locus_tag="SCK08_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202234.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3451
                     /locus_tag="SCK08_002393"
     CDS             2951..3451
                     /locus_tag="SCK08_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1202235.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            3548..5395
                     /locus_tag="SCK08_002394"
     CDS             3548..5395
                     /locus_tag="SCK08_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEG1202236.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            5398..6294
                     /locus_tag="SCK08_002395"
     CDS             5398..6294
                     /locus_tag="SCK08_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1202237.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            6344..6733
                     /locus_tag="SCK08_002396"
     CDS             6344..6733
                     /locus_tag="SCK08_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1202238.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            6720..>9048
                     /locus_tag="SCK08_002397"
     CDS             6720..>9048
                     /locus_tag="SCK08_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1202239.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         3196 a         1362 c         1909 g         2581 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacgcga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcttactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tacgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc cgtcgatttt gatgaagtga aagaacggat
     3061 tggattaaat gacgaatatg aagagtacgc aattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acaccaattg aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa
     3181 aggaacgcca attggtgagg tcgcttcaga gattcaacat gccttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatatcat ctattatccc gattgtaatg acatgagtga
     3301 tgtagcgtat tacttgattg tagaagctgg tgatttaggc gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtcg agacttggag attggcggga attatttggt
     3421 tacgagtcga ggggtttttg aactaatgta aatcatttgt tagaattgac cagaaatttc
     3481 tatcgtactt ttacttttct atttgatata ctgaaataga aaatatttgt tgataggggt
     3541 gttactaatg gttggcaata agtggtacaa atgtgatctt catattcatt caactgcgag
     3601 tcagtgcttt caggataaaa aggttactgc taaggaatgg gtagaagaat gtttaaacaa
     3661 aggactcgat tgtgttgcat taactgatca caatacaggg gaaaatattg atgaatataa
     3721 gaaagtggct atcgaaaatg gcttagattt ttttcctgga gtggaattaa cgtgtggaga
     3781 aaatggaact catttactag tcctatttga taaaagtgca tgtacaaatg atatctctga
     3841 tttcttaacc aagatgggta taaaaagaga ggattgggcc agcgctgcag cttgctcaat
     3901 gaaatcagtt ttagaagtta ttgattttgc tgagcacgag ggcttgctcg ttattcctgc
     3961 acatatagat gagtttaatg gaatttcttc agcaaaattt ggaaatctag gaaagatatt
     4021 tgaatcagaa aatatatatg cagtacagac ggttcaaaaa gaattttttg aaaatcgctc
     4081 acaaatgatt ccctcatcta agcgtgacac tatctatgat agtgtgaatg ccagatatga
     4141 tggtagagtt ggaaaagata ctttggaatc ttggtataaa tcagttgttg aggcagaaaa
     4201 aaataatatg acatttctta gcttttctga taacccacac tcgaaaggaa attcaaaaca
     4261 tggactttgg ggaataggca caagatattc ctatataaaa atgaaggacg aacctgactt
     4321 gggatcactt cgcgatgctt taatgctagg cgaaactcga gttcattctg atttttctaa
     4381 tttctccatt aatgaaaatg aagttttatt ggaaaaatta agcttttctg ggaccactct
     4441 tagcacaaaa gaagttgtag tcgaattcag cgataattta acttcaatta ttggaggaag
     4501 aggaacaggc aaatcttgta ttacaagatt cttggtctat gtccttggaa aggaagcaga
     4561 acttgatcaa ttttctgaaa tacaaagtga ttatcagaac tttgcacaaa ttgaacataa
     4621 tggctctgga atatttttga aggatacaat tgtgaaactg aatattttct ataaggggac
     4681 aaaatatcaa ataataagaa cacaggatag acattctatt tatgaattta cacaagagaa
     4741 tggtctggtt gaaaaagaaa cagaacggtt gagaatgatt tccgataagg tgagtatata
     4801 ctctcaaaaa caaatttatg aaatttcaaa aaatcaaact agtatactag aattactaga
     4861 tggatataac tctgatttga tttcagagta taaaaatgag attgaaacat gtgtaaacga
     4921 aataagaaaa ttaaattggg atattgtttc tgtgaaaaaa gaaatacaag ataaggcgaa
     4981 ggtagagctt gaaattgaag atttaaaaaa acaagtagaa aagctatctc ataagagtta
     5041 taaagatgtt tatgatgagt attcaaaaga gacagatatt tatagggaac tgaaacgtga
     5101 tgtggaggca cttaaggaaa ttcccaaaaa tctaactgat agtgttgaca aaattgactt
     5161 tggcaatggt ataaaagtta cagacgaaat tgatgaacac cgcggagaat tgattaagaa
     5221 tttagtgacc aatcgggcta agattcaaac aataattaat gaaatgtcag atgaaattga
     5281 tagttataga gcagagctga ataaatcaga ttggaaagtt aactataatg aggtttcaaa
     5341 gaggtatcaa agaggtatca aaaacttagt gaagagttat ctcaagatga attaaaaatg
     5401 ttacaaaacc ttgattcttt atatcaatct ttatcaaaaa ctgaagagcg attggaagaa
     5461 attattcaac aagaaataaa gcttaaagat taccaagttg aggttgactc agcaatgtca
     5521 agaatgaata aatcatatca aaatctctat tctgagagaa gtagttcctc gcgcgaaatt
     5581 ttttctgata ttacaggaat agaggctact attcaaccaa atagggattt caataattat
     5641 gttaaagagc tcagagcttg tattaataag gaggacactt ttgacagtga gtttggatct
     5701 cttattgata agctgacaaa aaattccatt actaatcaag atatatataa tgacatactt
     5761 gatataagag ttagtgggga aagtagtatt ttttcagata aaaaattaat caattctata
     5821 agaaaattga atgatgcaca agttcttgaa ttacaaaccc ttaagccaaa tgatttagta
     5881 gttatccgac ttgaactaaa tggaaaaaaa gttagtttat cgaatgctag tgctggtcaa
     5941 aagacatctg caattctttc tatgattttg gcgtatggaa attcaccact catattggat
     6001 cagcctgaag atgatttgga tagtcaatta attaataact taatcgtaag aagtatcatt
     6061 acaaaaaagg aaaacagaca aattttaatt gtaactcata atgcaaatat cccagttaat
     6121 ggtgattcag aatggttgat tagcatggga gattcaaaag aaatatcggc tgatcagtgc
     6181 gggtcggtag atgaacccaa tatcaagcca agaatatgta ctgtgatgga aggtggcgag
     6241 gatgctttta caaatagagc gaaacgctat ggttttaaac aaattgtaga ataacactag
     6301 agggtgagac atattattat gtccgccctt tttaggagga aaaatgaaaa aaataaaaag
     6361 ctataccagt atctgggcag ttgaaaaagt catttacgca atcaatgatt ttcagttgcc
     6421 ttttcctgtg acttttaacc aaatggcttg gttcgtttta tctttactgt ttgtcatcgt
     6481 atttgcccat gtgcctccac tatctatgat agaggaggtc tttttaaaat acttaggcat
     6541 accggtggct gtcacttggt ttatgtcgca aaagacgttt gatggcaaga aaccactggg
     6601 atttttacga tcctttatca gctatcactt gcgatttaag gtgacttttg caggaaagaa
     6661 agtcaaagaa cagaagaaac gatgggatga acccagtact cttgtaagga gtgtgaacta
     6721 tgtatccgat taagtatatt gaaaataatc tcgtctttaa ccaagaaggc gagtgttttg
     6781 cctactatga attagtgcct tacaattact cctttttgtc acccgaacag aaatttcaag
     6841 tgcatgataa ctttcgccaa ctaattgcgc aaaatcgtga aggcaagatc catgcgttac
     6901 aaattgccac agaaagtagt attcgcgcca cacaagaacg atcaaaaaaa gaaattaccg
     6961 gtagactcaa agaagtagcg aaacaacgaa ttgatataca gacagatgca ttagtatcca
     7021 tgattggcga tagtcagatt gattatcgct tttttattgg ctttaaacta attgcgacag
     7081 atgaagaagt caatctaaag agtctcaaga aatctttctt ttctgggctt caagaatttg
     7141 tctatggagt gaatcatcat ttgatgggcg attttgtttc cttatccaat gaagaaattc
     7201 gctgctattc taagcttgaa aaattaatgg aaagcaaatt ggctcgaaga tttaaagtca
     7261 ggcgagtcac accgagtgac ctgacgtatt tgattgaaca tatttatggc gaaaaaggga
     7321 taccgtttga agaatacgaa tttcagctac caaagaaaaa gctaaaatca gaaacactgg
     7381 tgaaacgtta tgacttatta cgtccaaatc gttgtttaat tgaagaaaaa ccaaggtgct
     7441 tacggatgga acatgagaat catgagtcgt acgtagcgta tttaacaatt aatacaattg
     7501 tgggggagat ggaatttcct tcctcagaac ttttctatta ccagcaacaa caatttacat
     7561 tcccaatcga cacaagtatg aatgtagaga ttgtgacgaa taaaaaggct ttagctaccg
     7621 ttcgtaataa gaaaaaagaa ctgaaagatt tagacaatca tgcttaccaa tctgacaatg
     7681 aaacgaattc caatgtctta gatgctttgg attccgtgga tgaattggaa accaccttgg
     7741 atcagtcaaa agaatctatg tacaaactga gttatgtggt tcgagtgagt gccgaatcag
     7801 tggatgaact aaagcgacgc tgtgatgaag tgctcgattt ttatgatgat accaatgtaa
     7861 aactagtccg gccctttggc gatatgatgg ggctacatga agaattctta ccctcaagta
     7921 aacgatatat gaacgactat attcagtatg ttacgtctga ttttttggcg gggcttggtt
     7981 ttggggctac tcagatgcta ggagaattag aagggattta ctttggctac aatgtggata
     8041 caggtcgtaa tgtttacctg aaaccggcat tggcttcaca aggagtcaag ggttcagtaa
     8101 ccaatgcgct ggccgccgct ttcctgggtt ctcttggtgg aggtaaatcc tttagtaaca
     8161 atctcttagt ctactatgct gttttgttcg gtggtcaagc tgtgattgtc gatccaaaag
     8221 gggagcgagg agggtggaaa gaaaccttgc cagaaattgc ggatgaaatc agtatcctca
     8281 acttgacgag taagtcagaa aatcaaggtc tgcttgatcc ttatgtgatt atgaagaaaa
     8341 agaaagactc ggaaagttta gcaattgata ttctgacttt tttaacagga atttcaagtc
     8401 gagacgggga gaagtttcct gtgttacgta aagccattcg aacggtgact caaagtgatc
     8461 aacgggggtt attacaagtt attaacgaat tgcgaaaaga cgggtcacca atcgcggaaa
     8521 atatcgccga tcatatcgag tcctttaccg attatgattt tgctcatttg cttttctcag
     8581 acggctcgat tacccaatcg attagtttag ataaacagct aaatatcatt caagtggcgg
     8641 atttggtttt accggatgca gaaacgactt ttgaagaata taccaccatg gaattgctta
     8701 gtgtttcgat gctaatcgtc atttccactt ttgccttgga ctttatccat agtaaccgag
     8761 aaatcttcaa aattgtcgat ttggatgagg cttggagttt tcttcaagtg gctcaaggaa
     8821 aaacgttatc gaataagctt gttcgtgctg gtcgggccat gaatgctggg gtctatttcg
     8881 tcactcaaaa tagcgacgat ttattagatg aacgtttaaa aaataatatc gggttgaagt
     8941 ttgcttttcg cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggtttgg
     9001 atcaggagga tcaaagcaat caaaaacgcc tgcgagattt ggaaaacg
//
LOCUS       DARBFD010000104         8933 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000104 DARBFD010000000
VERSION     DARBFD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.106"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(93..761)
                     /locus_tag="SCK08_002398"
     CDS             complement(93..761)
                     /locus_tag="SCK08_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1202240.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="SCK08_002399"
     CDS             895..1182
                     /locus_tag="SCK08_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202241.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="SCK08_002400"
     CDS             1598..3136
                     /locus_tag="SCK08_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEG1202242.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="SCK08_002401"
     CDS             3133..4098
                     /locus_tag="SCK08_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1202243.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="SCK08_002402"
     CDS             4085..5407
                     /locus_tag="SCK08_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1202244.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="SCK08_002403"
     CDS             5648..6391
                     /locus_tag="SCK08_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1202245.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="SCK08_002404"
     CDS             6406..7398
                     /locus_tag="SCK08_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1202246.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="SCK08_002405"
     CDS             7410..8147
                     /locus_tag="SCK08_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1202247.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="SCK08_002406"
     CDS             8137..8859
                     /locus_tag="SCK08_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1202248.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2980 a         1518 c         1868 g         2567 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aat
//
LOCUS       DARBFD010000105         8889 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000105 DARBFD010000000
VERSION     DARBFD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.107"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            45..1106
                     /locus_tag="SCK08_002407"
     CDS             45..1106
                     /locus_tag="SCK08_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1202249.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1109..2005
                     /gene="murQ"
                     /locus_tag="SCK08_002408"
     CDS             1109..2005
                     /gene="murQ"
                     /locus_tag="SCK08_002408"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG1202250.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2019..4001
                     /locus_tag="SCK08_002409"
     CDS             2019..4001
                     /locus_tag="SCK08_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1202251.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4066..4512)
                     /gene="nrdI"
                     /locus_tag="SCK08_002410"
     CDS             complement(4066..4512)
                     /gene="nrdI"
                     /locus_tag="SCK08_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG1202252.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4723..6147
                     /locus_tag="SCK08_002411"
     CDS             4723..6147
                     /locus_tag="SCK08_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1202253.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6328..7101)
                     /locus_tag="SCK08_002412"
     CDS             complement(6328..7101)
                     /locus_tag="SCK08_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1202254.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7098..8003)
                     /locus_tag="SCK08_002413"
     CDS             complement(7098..8003)
                     /locus_tag="SCK08_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1202255.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8157..8477)
                     /locus_tag="SCK08_002414"
     CDS             complement(8157..8477)
                     /locus_tag="SCK08_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEG1202256.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2809 a         1590 c         1885 g         2605 t
ORIGIN      
        1 tctagtcata gaaatgaaga cggatacaga aaaagaggga taatatgttt ggcttttcaa
       61 tatttatgaa cgattctttg tcagaagaga agaaacagta tatccgccaa atggcatcca
      121 atggctttgt cggtattttc acttccatgc atataccaga agatgatgtt tcggcatatc
      181 aaaagcgatt gatcgaacta ggaagcgaag cgaaaaagaa taaactagaa ttgatggtcg
      241 atatttcagg agaggcattg gaacgagcgg gtttttcatt agaagatcta acgcctttga
      301 aagcaattgg tgtaacaggt ttaagaatgg attatcatat ttccaatcag cagattgccg
      361 actggtcaca tcaattgaaa atcagtttga atgctagcac gatcacgcct aaagatatcg
      421 atgaattgaa agaagcagaa gctgatttct cacagattga agcttggcat aattattatc
      481 cacgtcccga aacagggctg gacaaagaat ggtatcagaa aaaaaatcaa tggctcaaaa
      541 ttcaaggtct ccttgtccaa ggttttgtac caggagatac agagttgaga ggcccactat
      601 atcaaggatt gccaactttg gaagaacatc gagggatcca tcctcttgca ggcgctctag
      661 atctattagc aagtaacacg gatattgttt acatcggaga tgcaggtctg tcagaaaacg
      721 ttcaggagca atttgcttct ttccaaaaag aacagacagt cttgctccat acggaacctg
      781 tagatgaaga attttacgaa tatatattag ggaagcatac gaatcggcaa gacgatgcaa
      841 gagatgtcat acgaagcgta gatgcacgtt ttcgagagat tccgcctatc ccagcaagaa
      901 atacagctac gcgtatgaaa gggtctatta ctttagataa cgaaaagtat ttgcgctata
      961 tgggtgagat ccaattaacc aaatatgatt tgccagcgga tgaaaaagtg aatgtagtag
     1021 ctaaagttat caaagaagag ctaccattga tcaaccaaat caaagctggt atgaactatc
     1081 aatttataag aaaagaagga agataaaaat gagcaaaatc aatttagaga acctaacaac
     1141 ggaacgcaga aaccaagaaa cttttggatt agatgagatg agtatttctc aagccgttca
     1201 actaatgaac aaagaagatc agaaagtggc cgctgcggtt gaaaaagaac tgacaagtat
     1261 cgaaccagtg attgctaaca cgattgagtc ctttaaaaaa ggtggacgtc tgatttatat
     1321 gggagccgga acaagtggaa gactaggtgt gttagatgct gctgaatgcg taccgacatt
     1381 cggtgtagaa ccggaaatgg tcataggtct aattgctggt ggagaagaag caatgactgt
     1441 agctgtcgaa ggggccgaag attcttatga gctagggcaa gaagatctaa tcaagctgaa
     1501 tttgacaaaa aatgatatag ttattggtat cgcagctagc ggtcgtacac catatgtgat
     1561 cggtggatta gattacgcaa aatcgatcgg tgctgtcaca ggaacgattt cttgcaataa
     1621 acaagccgaa atcagtaaac acgcggattt cccaatcgaa gtcgatgcag gtccagaatt
     1681 tttgacaggc tctactcgat tgaagtcagg aacagcacaa aaacttatct tgaatatgat
     1741 ttctactatc agtatgattg gtataggtaa agtattcaat aacttgatgg tcgatgtcaa
     1801 accaacaaat gaaaaactaa tcgaacgctc aaaacgaatc atcatgcaag cgacagaagc
     1861 tgattacgaa acagctgaaa agtactttga agaagcagat caaaatgtta agctggctat
     1921 cgtcatgatt ttgacaaata gtgataaaga ggaagctcaa gcaaaattag ctgaagcaaa
     1981 cggatttgta aaagggacct tatgaggggg aaaagaccat ggcaaaaaac gaactgactt
     2041 tggaagaact ggcaaaacaa atttataaag gggcaggcgg catgggcaat gtagaaagca
     2101 ttgtccactg catgactcgt gtgcgtatga agatcatcaa tgatgatctt gtaaatgtct
     2161 ctgaattaaa agcgattcca ggagtgcttg gtgtagtaga agatgaacag cttcaagtca
     2221 tcatcggacc tgggaaagtg aacaaagtag cacagcaaat ggttgatttg gcaggcgtga
     2281 aattaggaga aaaactttct tctgtaagta cagctacagc aaattcatca gcatctggaa
     2341 aagaccgtgt aaacgaacgg gcacaagaaa tgaaagcggc acaaaaatcg aaacaaaaac
     2401 catctaaaat caaatcggta ttaaaagaca tttctaatat ctttgtgcca atgattcctg
     2461 catttgtcgg tactgggatc gtggcaggga ttgccgcagt tttagcaaac ttagtcacag
     2521 ccggaacact tgatgcagca acatggcaac aatatatcga tgtgatgaac atcttgaaaa
     2581 acgggatgtt tagctaccta gttatctata caggtatcaa tgctgcacaa gtttttggtg
     2641 caacaccaac tcttggtgga gttatcgggg ccgttgtgat gctgacaggg atgaatccgg
     2701 aagcaccact gaaaaactta tttactggtg aagctcttgc agctggacaa ggtgggatcc
     2761 taggtgtcat cttcgctgtt tggctattgt caatcgttga gaaaaaactg cataaagtag
     2821 tacctgatgc ggtagatatc atcgttacac caactctttc attgattgcc atcggtctga
     2881 ttgaaatttt cttcatcatg cctttagcag gtttcatttc tgatggcatg gtcggtggga
     2941 tcaactgggt acttggcgtt ggtggagcat ttgccggctt cgttctaggc acattattct
     3001 tgccgatggt tatgttcgga ctgcatcaaa ttttgactcc aatccatgtg caaatgattg
     3061 atgaaactgg tcgtacgcta ttgcttccaa ttcttgcgat ggctggtgca ggacaagtgg
     3121 gtgcagcatt ggcattatgg attcgctgca aaaaagataa agaattaaca gaaatgatca
     3181 aaggagcttt accagtcggg atcctaggaa tcggcgaacc attgatttac ggagtaacat
     3241 tacctctagg acgcccattt attactgctt gtatcggtgg aggtatcggc ggggcagtga
     3301 tcggaatgat cggaaatgtc ggggctatcg ctattggacc ttcaggagcc gctttgatcc
     3361 cattgatctc tgacggtaaa tggttaggct acatccttgg attattggca gcttatgctg
     3421 gtggcttcgt tgctacattc ttcttcggta ttcctaaaga ccgtttagaa aaagaagaat
     3481 tagcgactga aacagccaca gtaaatgaag tgcctgaaaa agtagtacct caaacttcag
     3541 ctgaaagtgt gactatcctc tcatcagttg ctgacggaac agttgtttct ttagataaag
     3601 caagtgatcc agtttttgca caaaagatga tgggtgaagg ctactttgta gaaccagaaa
     3661 acggacatat ctattctcca gttactgggg tagtcagctc tgtcttccca acgaaacacg
     3721 ctattgggat cacaacgcca aatggtttag aaatcttgct tcatatggga atcaacacag
     3781 ttgacttagg cggtacgcca tttgatgtga aagtaacaga agggcagcaa gtgacacctg
     3841 atacgcttgt agcagaagct gacttagaag cgatcaaagc agcaggcaaa gaaacatcca
     3901 tgatcgtctt ggtaaccaat atggatcgtg taaaaaactt tgtcttggaa aaaacaggta
     3961 aagcaaaagc aaaagcacct gtgatggatg tcaaagctta atatgataga aacaatagaa
     4021 acgaaagaag aaactgaaat ctatttttca gcttcttctt ttttattatt tattgttttc
     4081 tgctaaaatc tgataaattc gtgtcacatc agatggtgtt ccccgtaatt cataatctgc
     4141 caagaaagga aagccaaatg tttgtgcata ttgtttggct gttagacaat actgattatt
     4201 gaagttttta tttccgctac cgactacacc aagacaaagt ttatgattgt cttcatgttc
     4261 cagatattca cgcatcgttt cggtcaagat ttctgtatca ccgttatcta aaccatttcc
     4321 gccgtctaaa taagtaggaa cgaaagtaaa aaatggttct gtttctttcg taaaatcact
     4381 attttcatgg atttctttta aagtaacttc aggattgaca ggatcttctg catgcatttt
     4441 ttcagcatat tctgctagat gtttggcaaa ggcacgcgta tttcctgaaa tcgatatata
     4501 taaaatgttc atggatcatc ctccaattct ttccttttag tatagcatct tttttaagtt
     4561 ttttggtggt gtaatttgga aagggactag gcgctttgct tgtataatga gaaaaaagcc
     4621 ggtagaatta tatttctatt aaagttttta ttaattaagt aaaaataaaa aaggtttgtt
     4681 atactttttg ataaaagcgg ttttattttg gaggatgctt acatggatac agagcaacag
     4741 aaaaaaggaa ttggtttaat tccacttgct gccttagtaa ttggttcagc gattggaggt
     4801 ggcgtatttg gtattatgac ggacatctcc ggatcggctg gcggcccagc tattttcagt
     4861 tgggtactcg taggaacagc aatgttgatg ctctccttgt cgatcaataa tatcaataaa
     4921 aaacgtccag atttaaaagg cgggatcttt agttacgcag aagaaggttt tggccgtttc
     4981 gctggtttta tcagcggttg gggttactgg ctcacatgct ggttaggcaa cgtagctttt
     5041 gcaacattgt tgatgagcgc attaggatat ttcttcccag tatttgaagg tgggcaaaat
     5101 ctggcatcga tcgtgatgag tacagtcttt ttatggggat atgcatggct agtgaatcgg
     5161 ggagtcgaaa atgctagttt cgtcaatgct atcgtcacat tctgtaaatt agtgccatta
     5221 tttttattta ttgtagtagc tatcagcacg ttttcgatcc aacaattcat tgataacttt
     5281 tctagcgaca ttatattgaa tgcgaatgga cagccgatcc cgttttccag tcaagtattg
     5341 agttgcatca tggtgatgct ttgggtattt gttggtattg aaggagcctc tgtcgtaggt
     5401 tcacgagcaa aaaaacaatc agatgttggg aaagcaacga ttctagggat attcggcttg
     5461 attattattt acgtcttggt ttccatgctt ccatatggga caatgacgat gaatcagctg
     5521 caaacgatca aggcgcagcc tgctatgggc tatgtgtttg aaatgatggt tggtaaatgg
     5581 ggagcagtcg tgatcaacgg cggtttgatc atttctttag ttggtgtttg gctctcatgg
     5641 acgatcttac cgattgaaac aatgggaaac atggctgaag acggactttt gcctaaagca
     5701 tggggaaaac tcaacaaaaa tggggcacca acttatgcta tcgtattgac gacgttatgt
     5761 acgaatgtct ttttactttc attattattc actgacagtg cttataactt tgcttacaca
     5821 cttggtacag cagctatttt cttcacgtgg cttttcttag gattgtacca aatgaaactt
     5881 tcttatcagc ggaaagaatg ggtccaattt ttagtagggc ttttagcaag tgcctttcaa
     5941 gtatgggcta tgctagtcgt agcctttaat gaagtaatgc ttgttcttgt tcttttcttg
     6001 ccaggcattt atttttacct caaagcacgt aaggagcaaa acgagcctgt acaacatatc
     6061 cacttagata agattttact tataggtgtg acgatggtcg gagtgattgc aatggtttta
     6121 tttgccactg ggatgcttca cgtatagaag tataaaagaa gtataaagag aggttgtgag
     6181 cctgtcgttt attcaaaaac atactaggaa aggagctgtg acacaaatgt tgtcacagct
     6241 cctttttcag aataaacggt gtttaagaga cagccaatcg gcaataaggc tgaacgtctc
     6301 tttcacagcc tcttttttga tgagtcatca tacatttttt cgcttaaata cgagatatcc
     6361 taatgctaag aacaaaacga tgtaagctaa attaccgaat agcatttgat tagtagaaag
     6421 atgagtcaat tgcttcatga tctcttcgtt tccaatttgg ttttgcagat tcaacatact
     6481 gatcggattc catttgatcc actcccattt ttggatgagt gcaataagga taccagatat
     6541 gattgacgaa gcaaaataaa agacgattcc tgctgagatg gatgcgccgg aactattgat
     6601 aaagcaagca atcatcaaca ctaaacttaa aatcagccat aaaccgatat aactacccaa
     6661 agtataagtg aacaagcttt ggatcagtgt ttgaccagta gaaaccttct ccgtgaagga
     6721 aatcgaaggg aaaagaatca gtttcatcaa cacagtgaca ataatagcta aaaggtataa
     6781 ataaacagaa taaataacta atgtgatcca tttgcttact aatatttctc ctcgagaata
     6841 ctttcgataa aggagatttt tcaatgtacc atattgtgct tccatcgaaa tgatcgtgct
     6901 tgcagctgca atcatgataa agacaatcca agatgtggca ctaaaaagct gagcagtcat
     6961 ttcaactgga tcaatgatcg tcgtgtattt tttggctaga agtgctgttc ctatgagcaa
     7021 aacaactaat agaacagata aaacagcagt tgactttttc ttgatcaatt taaataattc
     7081 ttgttttaca agcaatgtca tttttcttct tcccccttta ctgaatccag aatttccaat
     7141 agagaagatt ccaaatcatt cgtcagatgc tggacgtctg tcacatggat attttgtgca
     7201 gacaatagct ggatcacggt tcctaataat tcttcgtttt cttttaaaag cttgatttta
     7261 tcaggatctt ccaaagtata tccagcttct gtcaagatcg ttcgagcagc tagatcattt
     7321 gttgtagaga taacgatgaa atcttgcgct gtttggttca attcagccat tgtacattgt
     7381 ttgatgatct ttccttgatt gatcaggatc agctcatcga ccattttttc aagctcgctg
     7441 agtaagtgac tggagatcaa gaaagaggtg ccctgagtag ccaagtcggt aatgattttt
     7501 cgcacatctc ttgttgcttg tggatccaaa ccgttcatcg gctcatctaa aataacgata
     7561 gacggatggt tcagtaaggc aagagcaatt cccaattttt gtttcattcc caacgagtag
     7621 gatttcgctt ttttgtgaat gtacttttcc atcttgagct gattcacgat agtgtcgata
     7681 gcagatacat cgttggtttc agagaataac ttcagatgat catagcctgt taagaatgga
     7741 tagatacccg ggtattcgat caatgctcct acttgttcca accctttatg actagaggga
     7801 gaaatctcat tttcaccaat acagattgtt ccatctgagt aatggatcag tcctaaaata
     7861 gctttcatga tcgttgtttt tccagcgcca ttaggaccta ctaatccaac gattttgcca
     7921 ggggataagc taaaactgac atcgctgagg gcttgtttgt gaccaaagtt cttggataat
     7981 tcttggaccg aaagactttt caaaaatatt cactcctttt ctatagatag tacaagcata
     8041 cacctgatga gtggagaagt cgctaggtca ttggacttat ttcttatttt ttcataaaaa
     8101 aattgaatat taaataaaaa ggcttatttt ttctgatcag tacttattcc aagtgtctag
     8161 tggccgctag taatcttgat gccaatgata ctgatgagta gcatccccac aaagaaccaa
     8221 gtggaaagat tcagcttatc atgaaacaag accacaccaa ttatgaccga accaactgcg
     8281 ccaatccctg tccagatagg ataagagaga ctcatcggca aacttttgat tgcctttgac
     8341 aaaaagtaaa aactagcaat catgccaatc actgtgtaaa aagaataatt gattttagaa
     8401 aaaccatcac tccatttcat catcgttgac cagaatactt ccaaaattcc tgctatgatt
     8461 aattccatcc atgccatact tacattcctc cttaactata aaaaaataca caaaaagagg
     8521 aaaatcatcc cttcaaagac ctaatggaat gatcatcctc tttatcctac ccggtggcag
     8581 attgtatatc tttttctgta gtatagcaca cctatccaga tatcggcaat ctcatacttt
     8641 ttagtaaagt gtaccaagca ctgacggata tatcgttagc ggaaaagaag atgaatgctt
     8701 tgcaatttca tagaaagaag ttgttattta ttaatcattt aaaaaaaaca gtaactagac
     8761 cttatttcta tatattatcg ggcaataaat taaaaaaagc tttaccttac aataagtaag
     8821 ataatttttg tgataaaaaa ataacgaaaa atatttaaaa atagtgtcta tttcgtgaga
     8881 aaacaacta
//
LOCUS       DARBFD010000106         8572 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000106 DARBFD010000000
VERSION     DARBFD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.108"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="SCK08_002415"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="SCK08_002415"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG1202257.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1590..2936)
                     /gene="gor"
                     /locus_tag="SCK08_002416"
     CDS             complement(1590..2936)
                     /gene="gor"
                     /locus_tag="SCK08_002416"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEG1202258.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3070..4323)
                     /locus_tag="SCK08_002417"
     CDS             complement(3070..4323)
                     /locus_tag="SCK08_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1202259.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4329..5252)
                     /locus_tag="SCK08_002418"
     CDS             complement(4329..5252)
                     /locus_tag="SCK08_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1202260.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5473..7965)
                     /locus_tag="SCK08_002419"
     CDS             complement(5473..7965)
                     /locus_tag="SCK08_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG1202261.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7972..8439)
                     /locus_tag="SCK08_002420"
     CDS             complement(7972..8439)
                     /locus_tag="SCK08_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEG1202262.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2354 a         1814 c         1422 g         2982 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
     1081 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
     1141 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
     1201 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
     1261 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
     1321 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
     1381 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
     1441 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
     1501 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
     1561 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
     1621 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
     1681 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
     1741 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
     1801 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
     1861 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
     1921 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
     1981 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
     2041 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
     2101 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
     2161 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
     2221 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
     2281 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
     2341 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
     2401 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
     2461 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
     2521 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
     2581 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
     2641 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
     2701 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
     2761 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
     2821 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
     2881 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
     2941 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
     3001 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
     3061 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
     3121 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
     3181 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
     3241 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
     3301 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
     3361 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
     3421 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
     3481 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
     3541 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
     3601 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
     3661 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
     3721 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
     3781 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
     3841 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
     3901 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
     3961 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
     4021 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
     4081 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
     4141 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
     4201 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
     4261 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
     4321 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
     4381 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
     4441 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
     4501 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
     4561 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
     4621 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
     4681 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
     4741 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
     4801 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
     4861 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
     4921 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
     4981 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
     5041 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
     5101 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
     5161 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
     5221 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
     5281 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
     5341 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
     5401 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
     5461 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
     5521 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
     5581 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
     5641 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
     5701 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
     5761 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
     5821 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
     5881 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
     5941 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
     6001 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
     6061 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
     6121 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
     6181 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
     6241 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
     6301 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
     6361 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
     6421 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
     6481 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
     6541 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
     6601 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
     6661 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
     6721 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
     6781 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
     6841 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
     6901 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
     6961 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
     7021 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
     7081 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
     7141 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
     7201 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
     7261 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
     7321 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
     7381 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
     7441 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
     7501 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
     7561 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
     7621 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
     7681 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
     7741 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
     7801 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
     7861 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
     7921 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
     7981 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
     8041 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
     8101 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
     8161 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
     8221 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
     8281 ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag
     8341 gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat
     8401 atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta
     8461 atattgacct taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa
     8521 ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DARBFD010000107         8494 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000107 DARBFD010000000
VERSION     DARBFD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.109"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..173)
                     /locus_tag="SCK08_002421"
     CDS             complement(<1..173)
                     /locus_tag="SCK08_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1202263.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="SCK08_002422"
     CDS             complement(593..1714)
                     /locus_tag="SCK08_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEG1202264.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="SCK08_002423"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="SCK08_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEG1202265.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="SCK08_002424"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="SCK08_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEG1202266.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="SCK08_002425"
     CDS             complement(4304..5215)
                     /locus_tag="SCK08_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1202267.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="SCK08_002426"
     CDS             complement(5232..6239)
                     /locus_tag="SCK08_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1202268.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="SCK08_002427"
     CDS             complement(6236..8365)
                     /locus_tag="SCK08_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1202269.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="SCK08_002428"
     CDS             complement(8370..>8494)
                     /locus_tag="SCK08_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1202270.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DARBFD010000108         7933 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000108 DARBFD010000000
VERSION     DARBFD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.111"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1283)
                     /locus_tag="SCK08_002429"
     CDS             complement(<1..1283)
                     /locus_tag="SCK08_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1202271.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
     gene            1712..2245
                     /locus_tag="SCK08_002430"
     CDS             1712..2245
                     /locus_tag="SCK08_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202272.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(2371..3048)
                     /gene="nth"
                     /locus_tag="SCK08_002431"
     CDS             complement(2371..3048)
                     /gene="nth"
                     /locus_tag="SCK08_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEG1202273.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3055..3753)
                     /locus_tag="SCK08_002432"
     CDS             complement(3055..3753)
                     /locus_tag="SCK08_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1202274.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            4021..5205
                     /locus_tag="SCK08_002433"
     CDS             4021..5205
                     /locus_tag="SCK08_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEG1202275.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(5211..5654)
                     /locus_tag="SCK08_002434"
     CDS             complement(5211..5654)
                     /locus_tag="SCK08_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1202276.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5880..6167)
                     /locus_tag="SCK08_002435"
     CDS             complement(5880..6167)
                     /locus_tag="SCK08_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202277.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(6338..7690)
                     /locus_tag="SCK08_002436"
     CDS             complement(6338..7690)
                     /locus_tag="SCK08_002436"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEG1202278.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT         2171 a         1539 c         1360 g         2863 t
ORIGIN      
        1 ttcttagttt tcttgaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga
       61 aagtttctaa agccataggc aattcgtttt agtactttga tatggttgtt caaacattct
      121 atagggccgt tggaataggg aactcgcaac gctcgtttga tttctgttcg atatttcttg
      181 aaggtcgtga acactggttg atagtgctct ggcaactcct tgtagtcttc ttttaggagc
      241 gcgacgaatt caggaacatc ttgtcttctg atagcggaaa gaaaaacctg gtagacttcg
      301 tagcctcgct ttaaggagtc atcataatat aatagccgat cgactatctc cgcttctgtc
      361 agatgatctc gaaatgacgg acgccagtag cgatgctgac cattcaattc ccacgcattc
      421 ttttgaagta gtctccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag
      481 gtcttcatta cttgaattcg gtggttggta aaggcccgac cgatgtgttg gctaatatga
      541 aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt
      601 gtatagaaat ctgaaaccac tagacgtact tgttcacgga cttttctagg gtagcgcaag
      661 aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc
      721 ttgccgtcca tcatgacaaa gctcatggct cccgagacgt ttttcaccga tttgaattca
      781 tcgaaacaca ccacttctgg taacggctgg ttcaaagaaa cggttttagg ccgaaggctg
      841 cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcaat caaagacata
      901 gaaataggct cacttaatag ctcaagaatg gcttgtttga ctctgcgggc aatggagcag
      961 cgacgatcgc taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag
     1021 cgctgtttgt taatccgcac aatggtcttg tagttactga catcattcaa taagatggat
     1081 acttttttcc agccccattt aatgatcgtt tgcttatttt gatagtggca atggaagcat
     1141 tcctctggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attaatgcgc
     1201 tctttgcgaa aggcatcttc atgaaaggta agatttaggt ctagaatatc tagtaactct
     1261 ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta
     1321 ttctaaagga tttcaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa
     1381 cagaaggcgt caccaacact agatattata gatcctaaaa aagaaaccac gtctacaagc
     1441 gtggtttctt ttttgtgtct atgtaaaaaa cagctacaaa taaaaatcat gtaccaagct
     1501 gtattacatg attttttata agactatcca gcatatacat gtgtgatcta acatttagct
     1561 taaaaattag aaaatccatt tttcttttat cattacatat actttatcca tttacttgtt
     1621 tataattaaa tcggctaaat acacactagt cttttattta gtgccacttc ttaataatcc
     1681 cattgttatt atcaaataaa aaatacaata tatggttttg aataattttt tgaaagaagg
     1741 cttcattttg agttatctga tagtatgctt aattatcata atttgtctgc accatctttt
     1801 tttatcgaaa acaatacctc gttattccaa gaataaaaaa gctgaaaaat tttgtttatt
     1861 attaaataaa tttactttaa ttgctcctat ccttgcattt attatttttt ctgtactttt
     1921 aagcactact cttaaaggaa aatttatgga aagaagctct catgcaatga ttctcacttt
     1981 tttatggttg ttgttcacca gaatctatat ttttctgatg agcctgaaac cgcccaaatc
     2041 cattagttta tgtttagtta taaatggtat ttttttatta tcacttatca tttttattac
     2101 acctttagat agatacgtca cttatttata taatcctttg gaatactgga cttattttat
     2161 aggtatattg gaaggcatta ttttctacat cgggtatttt cccaataaaa ataacaactt
     2221 ttatttttat cgcaataaat tgtaataagt taccatccta gctactgagt atgtacattt
     2281 actttttcat gcaaaaaaga taaacgaaac agcttttctc cataataaca agataattca
     2341 tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt ggtttctttt ttagcgcctt
     2401 ttcttttaga cgcatccgat ttttcccatc ttgacatatt gatagcaaag gacatacctc
     2461 gcacttggga tttcttgccg tgcaatgata tctaccaaaa aaaatcagtg tatgatgtgt
     2521 ctttacccat aattcttgtg gtactttccg catcagtgtt tcttctactt ccatgaccgt
     2581 agcatctagt ttgcagatcc tcaggcgttt tgagacacgt tccacgtggg tatctactgc
     2641 gattgctggt atcccgaatg catcccccaa tacgacattt gctgtctttc tgcctacacc
     2701 aggaagagac atcaattctt ctcttgacgt tggcacttgc ccatcgaaac gttcaatcag
     2761 ctgctgtgca cacgctttga tgttttttgc tttatttcga tacaaaccaa tcgtttttat
     2821 tttaagaatg atttcatcga ttgaagcttc tgctaacgca tcgggtgtcg gaaaagaggc
     2881 aaacaaatct ggcgttgctt tattcactga tacatctgtc gcctgggcac ttaagatcac
     2941 tgcaatcaat aactcaaatg gattattgtg cttgagttct ccatgtgctt cgggaaacat
     3001 tccatacatc gtttctagag cttccattgt tttttgtttg cttaacatat tccgctactc
     3061 ctccaaccaa tttttcagtg agactttcgg caattcttct tgttgagctg actgttctac
     3121 ttctctttga agaagctgct gttttcttcg tttttggtct tcttccactt gttgttttgt
     3181 acgtagattt tttctttccc acgatagcaa aatgcggtca acgtaattaa aactataagc
     3241 ctgatttaat acggcttccc gcagtgctag cttcaaaatt tccggctgat aatggtcttc
     3301 ttctagccat tgtccgatcc gctggaactc gatcgaagaa agcgggcgtc caaattcttg
     3361 ttcaaataat tgatacactg actggatttc tttttcttgt gttttttctt cgtatttttt
     3421 ttcttgtgtt tttaaatact cacctaatag atcatagatg gggtaaagat tatatcgatc
     3481 tgctttttta ccgttgacaa tcgtcgtttc tatcttgata aatccatttg ttacgagacg
     3541 atccagtatt tgaaaaatct gatcttgttt gattcccata tccatagcaa tcatctgcaa
     3601 atcagggaaa gtatctcctt caagctgtgc catatgcaac tgtagaacaa ataaaaattc
     3661 ttcagattgt agaccgatcc tatgataatt tttaagtaaa agattagata tgactgtttg
     3721 tccagcgctt aagtaatttt ctaaattcaa catgatttct cctccgctca atcacagtat
     3781 acgtaaaaaa aacatgggaa gcaagaacag gtttcttttc tatttacttc actgatcggt
     3841 cagatttaca atgtcgtcta cttgattttt gtttgttcat gtttttgatg cgtatcttca
     3901 cttttcggaa taaataaatt aagtagattt cgctgttcct tagaatgttg acaaggaaac
     3961 aaaattacta tacactattt ttgtttccaa gttaacatta ttgcataaag gagtttttac
     4021 atgtacagtt tcacaaataa acattctaaa gtttttggat acgtctctgt cttattcact
     4081 ggattaggat ttactatcgt cagtcctgta cttccgtttt taacgctgcc ttattctcac
     4141 tccatacatc agcaagcttt ctatatcact ttgctcatgt ctgtttatgc tctagctgcc
     4201 ttcttatctg cccccattct cggtagctta agtgatcatt ttgggcgtca tccgatcctt
     4261 attatcagtt tattaggatc aagtatcgga tatttgcttt ttggtttagg aaattcgata
     4321 tggatgttgt tccttggtcg tatcattgaa ggattgacgg caggagaaat ctccacctta
     4381 tatgcttact ttgccgacat cacggaacct aatgaaagaa ccaaagtttt tggatggatg
     4441 ggtgctttag tcggtatagg aacaacgctg ggtcctatta tagggggtct attagctgag
     4501 ttggggaatt ctgttcctat atttattgga gcactcttta ctttcttaaa tgccgtttat
     4561 ggctacacat ttatgctaga aagccttcca ataaaaaaac gttcagtaga tttaagtttc
     4621 tcccatgtaa gaccttttca ccaattacaa caattattca agatttcatc cgttattcct
     4681 ttattgacag caggttttgc tgtttggttg gcagctggct ctcttcaatc tatcttttct
     4741 caattttcga ttgacgcctt tcagtggaag gctgggttgg ttggtttaag cttctctttg
     4801 attggaatct tggatactgt ctcgcaattg ttcattatgc ctcgattgct caaaaaattt
     4861 tcagaacagc agatcacaag aatagggatg ttcagtgaaa tcctcgccta tctattcatc
     4921 acattatctg gaattcttct actgccgatt ttgtttttat ttgggattat ctgttatggt
     4981 ttcggtgatt ctatttttac tcctgtattc aatggccaac tatccaattc tgtctcagat
     5041 aaccagcaag gtcttgtaat gggcggtacc caaagtatac aatctctatc gcgcgtcatc
     5101 ggcccgctaa tcgcaggaca gctttatgct gcagcaccat ttcttccgac ggtattcgga
     5161 tttttgctta tgatttttgc ctattatcac tacaataaaa aatagtagct tcatttgcta
     5221 cttcgtataa attctctgta tcgaccgata aaacgaaata ctgtgtcaaa ttcttctttt
     5281 gacatatttt caaatacagt agcatctcgc tgtacccatt tttgatggag ttgctcgtgt
     5341 ctattcctta ttttttgtcc gattggcgtc aattgaaaat agatttcttt tttattctct
     5401 tcgctttgat aagtgtttat gatccctcta gctatcagtt tttttgttag cttactgatg
     5461 gctcctctag tcatgtaact agcattcgct aattttgtca cgttaggatc agggattttc
     5521 ccgatatgtt caatcacttc tagttcattt agagtaaatc ctgcaaattc tttttccatt
     5581 tctggtttat tacgatgaat aatctcatta gctaaatcca gatattcttc tattagcagt
     5641 tgtcttttat ccatttctac accttctttt tctttcatta tattgttttt ttgtttccta
     5701 ttcaacaaag ataaaaatat tttgtaggaa aacccaatct gaaaccaaga aaaaaagggg
     5761 ttgacaaagc ttgtcataac ccctttttct gattaaaaga tagtaaagag acagtcatcg
     5821 gtaattgtga tgattttttg tcatcactta aggatgagcg gccttttcaa aaactgtctt
     5881 taaagatcat catcagtttc tagcacatat ggctgacctt catatgtgta ctctgtattc
     5941 aatgtgccgt cattttcatc tgagtagatg cgtctactga aaggaaaacg atgagaaaca
     6001 ttatctttgg cgtcatttgc tggcatcgtg taatattcct cttgcgtttc attcaactga
     6061 gagagtactt gatctgccaa ctcatcaata ttgatcgtta atttcttcga agcactttta
     6121 taatcttcta aaaatgcatc ttgttggaca aaatattctt tattcatctt ctttctcacc
     6181 tttcttttca aaatcaaata aagctgcttg ttcttttatg taacagcttt atctttgctg
     6241 cttattgtga caaaaaattc gaagaaagac aactaattgt acttttcaca aaaaaagaca
     6301 gagggtcgtg acaaaattgt gtcacaaccc tttttcttta ttgtgcagtt tcgatttcgc
     6361 ctagcgctgc ttgtgcaagt aggttcaagt aattccatgg gcggtcaaaa tgtggttgga
     6421 agaagaaatc tgaaagtgcg agatcttcta cagtcatttt attttgaaca gctaaagaca
     6481 tggtatttgc tgattgtgta atgtcgtatt tggacatgaa ctgagcacct acgatacgat
     6541 tcgttccttt ttcatagact agctccatca ttactttttc agtagttggc atgaactctg
     6601 gacgataatt atcttcaaat acagtcgctt ggacttctag atcgttcaaa gttgcgcttt
     6661 ctttggtcac acctgtcgaa ccgatcgtcc agccaaatag gtaaagtcct gaagttcctt
     6721 gagtaccacg atacgccatt ttatgatctg tcaagttatt tcccacaagc aaaccttgac
     6781 gaacggcatt tgttgccaaa ggaatatagt tttgcgtgcc acttggattg tagttgacta
     6841 ctgcagagtc tccagctgca aaaatgtctg gattacttgt ctgcatgtat tcattcactt
     6901 tgattgcccc gtttggcagt gtatctactt ttccttcgat cagttttgta tttggacgga
     6961 agcccacaca taaaatgacc atatctgtat caaatgtatc tgttggtgta gctacttgtt
     7021 tcacttttcc ttcttcatct gtgataaatt cagtaacatt ttcgcctagc gcaagagtaa
     7081 caccgcggtc tgtcaattct ttttctaaga tatctgtgaa tggtttatct agatatttat
     7141 tcaagatgcg atctaatcca tcaatcaatg tcacttgttt tccagattcg acaaaagctt
     7201 ctactaattc aataccgata tatccaccac caacaattac tacacgtttt gcttctttcg
     7261 cttgtgcaat aatttcattt gcttgattgt agtttttgca taggagtaca tttttcgcat
     7321 cgattccact aatcggtggg ataataggcc atgaaccagt tgtcatcact aatttatcat
     7381 aagaaaccgt ttctgtttct cctgttttca gatcttttgc cgttactgtt ttattttctg
     7441 tatcgacgtc tgttacatcg tgttccattt taactgttgc tcctaatgaa gataattctt
     7501 caggatttga gtagaacaag cctgctgggt cctttactac tccgccgaca taaagtgcga
     7561 taccgcaaga taagaaagaa atattgtcat ttctttcata tactgtcact tctgcttctg
     7621 gatgattttt taaaatactt tttacagctg cagtacctgc atgagtacag ccaattacta
     7681 ctactttcat ttatggtccc tccgattaac attttgtcct tcgacaaaac tactataaca
     7741 gaaataaaac ggatacaaca gtttaatgct tgtgaattca caaaggtttg tttgtagttt
     7801 cattcctttt caagcataag aacccaatat ctacaagatt cttattttaa taatattcac
     7861 ttttatatct aagattgtga ataaaagaca aaaaaataaa aaagctagcg ttttttttac
     7921 acagctagct ttt
//
LOCUS       DARBFD010000109         7862 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000109 DARBFD010000000
VERSION     DARBFD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.112"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(21..995)
                     /locus_tag="SCK08_002437"
     CDS             complement(21..995)
                     /locus_tag="SCK08_002437"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1202279.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="SCK08_002438"
     CDS             complement(1075..1950)
                     /locus_tag="SCK08_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEG1202280.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="SCK08_002439"
     CDS             2080..3987
                     /locus_tag="SCK08_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEG1202281.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="SCK08_002440"
     CDS             complement(4528..7806)
                     /locus_tag="SCK08_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEG1202282.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2217 a         1662 c         1462 g         2521 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 ta
//
LOCUS       DARBFD010000110         7859 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000110 DARBFD010000000
VERSION     DARBFD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.113"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            350..823
                     /locus_tag="SCK08_002441"
     CDS             350..823
                     /locus_tag="SCK08_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEG1202283.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            820..2952
                     /gene="feoB"
                     /locus_tag="SCK08_002442"
     CDS             820..2952
                     /gene="feoB"
                     /locus_tag="SCK08_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEG1202284.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2954..3088
                     /locus_tag="SCK08_002443"
     CDS             2954..3088
                     /locus_tag="SCK08_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEG1202285.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3182..3826
                     /locus_tag="SCK08_002444"
     CDS             3182..3826
                     /locus_tag="SCK08_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1202286.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            4013..5206
                     /locus_tag="SCK08_002445"
     CDS             4013..5206
                     /locus_tag="SCK08_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1202287.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5199..6926
                     /locus_tag="SCK08_002446"
     CDS             5199..6926
                     /locus_tag="SCK08_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEG1202288.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7320..7517
                     /locus_tag="SCK08_002447"
     CDS             7320..7517
                     /locus_tag="SCK08_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEG1202289.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7519..7830
                     /locus_tag="SCK08_002448"
     CDS             7519..7830
                     /locus_tag="SCK08_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1202290.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
BASE COUNT         2599 a         1316 c         1711 g         2233 t
ORIGIN      
        1 actcttagta aaaaagtcaa acaacaaaca ctacatattg tgttgtggaa actgtggaat
       61 actaagtttt gtgtttttga aaaaagagct gaaaacaagt atcgtgtggg aaaatagcaa
      121 atttaataga gaaaaaattg tggaaaacta tgaagaaata tttttttctc atttttatct
      181 atcaatacta aagattcgct cataatatca caagtataat tgacattctc aattaccgag
      241 agtattctat gtgagaatga ttctcattta tttgcgtaac acgtaagaaa attagaacat
      301 ataatgaatg attttaatac atgatttatt tcacaagaga ggaatcaata tgcagacctt
      361 agcgcaggca aagcagaatt tcgtttatca agtcgaacga atcgaaacag aagaaaaaac
      421 aaaacgccat ttgcaaaata tcgggatcgt tgccggaagt aaaatcgtcg tcgtcaattt
      481 atcaggcaac aatgccattt tactggtaaa aagtcatcga gtggcagtaa gtcgggaaat
      541 actaggtcaa atctttgtca ataaaaagac tgcttcctct gaaagctgga cttctcttga
      601 tcaattaaaa gcaggagaga ctgggatcgt cgtagcaatc cacggacaag gcgcagtgaa
      661 gcgacggtta atggatatgg gattgacgcg aaatgtaaaa atagttatcc gaaaactagc
      721 ccctctggga gatccaatcg aactgacagt gcgagggtat gaactgacgt tacgaaagtc
      781 agaggccgaa ctaatcttga tacaaaggga ggaaaaagaa tgaatatcgc gttagtcgga
      841 aatccaaaca gcgggaaaac tagcttgttc aatctcttga ctggttcgaa tcaatatgta
      901 gggaattggc cgggcgtaac agtagaaaaa aaagaaggat cgttgaaaaa aaatccgaac
      961 attcatattc aggacctacc cggcatctat tctttgtctc cttatacacc agaagaagtg
     1021 attgcaagag attatttggt tcaggaacag cctgaactga tcatcaacat tattgatgct
     1081 accaatttag aacgaaatct ttatttaact acgcagttat tggatttaga cattcctatg
     1141 atcattggtc tgaatatgat ggatgtcatc aaaaaagaag gaaaaaaaat caatctagaa
     1201 aagctttcct atggtcttgg tgtaccagtt ttaccaatca gcgtattgaa aaagcaagga
     1261 atcgaaaaac taatcgaaaa gattcaggag tctgcaaaaa agcggccttc tattcctgtt
     1321 ccctgtacat atgatccacg tttagaagca gctttacatg aaattgaaga tgtactagga
     1381 gaacaaagcg gcaagaaacg ctggtatgca atgaaatatt ttgaacgaga tcagaaggta
     1441 agagaggatt acgagatagc agtttctcag caaaaagaga tcgagcaact gatccagtta
     1501 acagagaaac tattggacga cgatagtgag acgattctgg tcaatgaacg ttatgaattt
     1561 attacccaac tatgtacact ctgtgttgtg tcaaatgatt ctttccaatt atcgatgagt
     1621 gacaaaatcg atcagattgc aacgaatcgt tggttagcct tgccgatctt cgcatttgtg
     1681 atgtggctga tctattatct ggctattcaa acagtaggaa caatggggac agattggatc
     1741 aatgacacat tgtttggtac atggttacca gaacatgttg gcagattgtt agctgaatgg
     1801 caagtagcgg gatggatgca ggagctgatc ttgaacggca tcatcgcagg cgtgggagca
     1861 gtgttaggat ttctaccgca attgattata ttgtttttat gcttgagctt tctggaagac
     1921 tgcggatata tggcgaggat cgcttttgtc atggatcgac ttttcaggaa attcggtctt
     1981 tcaggaaaat ccttcattcc tatgctgatt gctacaggat gtggagtgcc aggcgtcatg
     2041 gccagtcgca cgattgaaaa cgagcaagat cgtcgtttga cgatcatggt cacaaccttc
     2101 atgccttgtt ctgcaaagtt gccgattatt gccttagtag caggggcctt ttttcctcat
     2161 caatcttggg tagctccttc tgcctatttt cttggtatga ctgcaattat tttctctgga
     2221 atcagcttga aaaaaacacg tttgtttgct ggagatacag ctccttttat catggaattg
     2281 cccgcatatc attttcctca atggtcagcg gtcctaagac aaacctatga ccgcagcaag
     2341 tcttttgtta aaaaagcagg aacgattatc tttgtttcta gtatcgtgat ctggttcagc
     2401 tcttcttata atttttatgc tcaacctgtt tctgaagacc aaagtatctt agcctttttc
     2461 gggcgaatcc tggcagttgt ttttcagccg cttggttggg gaaactggca aggaacagtc
     2521 gcagcgatta caggattggt cgctaaagaa aatattattg gcacatttgg tattttgttt
     2581 ggtcatgcgg aagtatccga aaatggcaga gaaatctggc aagtgctaca acacacatat
     2641 actcccgcag cagcttattc attgttagcc ttcaatttac tttgtgcgcc ttgttttgcg
     2701 gcaattgggg ctatccatcg ggaaatgaat gccaagaaat ggacttggat cgccataggc
     2761 tatcaatgcg gtttagctta tttagttagt tttgtgattt atcagttggg acacttattg
     2821 gagggtggac aggtagcgtt agggacgtat cttgctattt tactgatgat aggactcgtt
     2881 tatgttttgt tccgtcagcc aaaatccaag aaccagtttt atactatatt gtcattagaa
     2941 ggagaggaat aggatggcga cattcttatt aagtgtgtta atctttggaa gtgcaggcgc
     3001 aattgtctat actcgtttaa aatccggtaa gaattgtgat gactgtcaaa cagcttgccc
     3061 agtaaaaaag gaacagatca agcgatgatc agtcgttgca agaggctatt ctcccctttt
     3121 ttgcttattt tgctagagca tagtaagatg agggaaagta aaaaaagaaa gaggaaagaa
     3181 aatgtctgga aagaaaaaaa tcgtccgccc gcgctaccag cagattgctg ttgatttagc
     3241 cgaacgtatc gttgaaaacc gatataaagt gggtgagaag atccatgccc gctcaacact
     3301 tgccagtacg tacaatgctt ctcctgagac tacccgcaag gctatcaatg tgctggtaga
     3361 tcttggaatc atggaagtac ggcatggaag tggggcattt gttgcttcga aggaaaaagc
     3421 aaaagatttc ctattgcaat atcaagatgt acaatccatc caagatacaa aagcagaaat
     3481 catggcaagt gtggagaagc aacaagatga gctgaatcat ttttctcaat tattggatca
     3541 gctggtcaat caaacgaagc gaatacataa gatgaatcct ttacttccgt ttgaattatc
     3601 tttatcagaa aaagcaatgc atctggaccg ttcaattagc gagttgaata tctggcaggc
     3661 gactggcgcc acagttatcg caatttctca taatgaagaa ctgcttgttt cacctggccc
     3721 ctatgctaaa tttacggcag gggacatcgt tcattttatt ggaaatgaat atacactaca
     3781 gcgtatgaca aacttttttt acccggagca tcagcttgca gaatgattgt gtgaaatatt
     3841 aaaaaaatat taacttggct aaacctttta tttacaaaga tttagccaag tttttttcag
     3901 tttggacaag caaatttctt tcatttttga caaagtgact acatgatgtt aacatagtgt
     3961 ggtcactttt ttcagaaaga aaaatgaatt tacataaaaa aggagaattt gtttggtaaa
     4021 agtagaagta aaacatttaa caaagatatt tggtaaaaag acacaagccg ctttagatat
     4081 gatgaatgat caccagccga aaacggagat tttaaagaaa accggtgcaa cggttggtgt
     4141 gtatgacgtg aattttgacg taaaagaagg agaaatcttc gtgatcatgg ggctttccgg
     4201 gagtgggaaa tccacattga tccgtctatt gaatcgtctg attgagccaa cttcgggcag
     4261 tatctatatt gatggagaag atgtatcgaa gttaagcaaa gaagaattgc gagaagtccg
     4321 tcgtcataaa atcaatatgg ttttccaaaa cttcggtctg tttccacatc gaacgatctt
     4381 ggaaaataca gaatacgggt tagaagtccg tggtgtacct aaagaagaac gacaagaaaa
     4441 agcagaaaaa gcattagaaa attctagttt gctttcattt aaagaccaat atcctagtca
     4501 gttatctggt gggatgcaac aacgggttgg cctagccaga gctttagcaa atgatcctga
     4561 gatcttgctg atggacgaag cgttctcggc tcttgatccg ctgatccggc gagaaatgca
     4621 agacgaatta ctggacctgc aagccaatgt tcaaaaaacg attattttca ttacccatga
     4681 tttgaatgaa gctttacgga ttggggacag aatcgccttg atgaaagacg gcgaaatcat
     4741 gcagatcgga acaggtgaag aaatattgac caatccagca aatgattatg tacgtgaatt
     4801 tgtggaagaa gtcgatcgtt ccaaagtgct tacggctcaa aacatcatgg tacctgcatt
     4861 gacgacgaat atcgaatctg atggaccaaa cgttgcattg acaaggatgc gtaatgaaga
     4921 agtcagcatg ctgatggctg ttgacagaaa gagacacctg aaagggatca tcactgcaga
     4981 tcaagcgtta gaagctagga agcaaaaacg tccattgatc gacttcctag atgaaaatgt
     5041 gacggtgatt gggaaagaca tggtggtaag tgatattttc aatattattt atgactcacc
     5101 gactccactt gcagttgtag aggatggaaa attaaaaggt gtcgtcatcc gaggtagtgt
     5161 gattgaagca ttagcagaaa caagcgaggt gagtgaacat gaataatcta ttgaactatc
     5221 aattaccagt ggcaaactgg gtagaaaata tcacagaatg gtttacgaca actttttctg
     5281 gcctattttc atttttacaa acgattggac aggcagtgat gtcggggatt acaaatttac
     5341 tgttagtgat tccagctcct ttgtttatct tgttattgac ggtagcagct ttctttattt
     5401 ctaagaaacg tccaggactt acgttgttca cattaatcgg tctatggttt atttataacc
     5461 aaggtctatg gaatgatttg atgaatactg tcacacttgt tcttttatct agtgtgatct
     5521 cgatcatcat tggggtacca ttagggatct tgatggcaaa aagcagtaaa gcccaaagta
     5581 ttatcaaacc gatccttgac tttatgcaga caatgccagg attcgtttat ttgattccag
     5641 cagttgcatt tttcggtatc gggatggttc caggggtatt tgcctcagtc atctttgctc
     5701 tgccaccaac cgttcgtttt acgaatttag ggattcgaca agttcctaaa gaattagtag
     5761 aagcttctga ttcattcggg agcacaagtc gtcaaaaact attcaaacta gaattgcctt
     5821 tagctaaaag tacgatcatg gcagggatca atcaaacaac aatgctgtca ctttcaatgg
     5881 tcgttattgc ctctatgatc ggtgcacccg gcttaggacg cggcgtatta tcagcattgc
     5941 aaagagcaca agtcgggaat ggatttgtca acggggttgc attagttatc ttagcaatca
     6001 tcattgaccg ttttacacag catttgaacc agccaaacaa taaaaaagct gcaggagcaa
     6061 caacacagaa aagtaaaaaa cgtcaaggac tgatcattgg tgctgtagta gtagtgatct
     6121 taggtgcgat tggtataggg agtttttcat ctgccaaaga aacaaaacgt atcaacttat
     6181 cttatgttga atgggataca gaagttgctt caacaaatgt agtcggtgaa gtattgaaac
     6241 aaatgggcta tgatgtaacg atgacgccat tagataattc aatcatgtgg aaatctgttt
     6301 caaatggcga gtcagatgcc atggtttcag cttggctacc taaaactcat ggttcacaat
     6361 atgcacaata taaagatcaa gtcgaagacc taggtgccaa tttgacagga gcaaaagttg
     6421 gtttagcagt gccagcctac atggatgtaa attcgatcga tgagttaacc gatcaggcag
     6481 gtaagaaaat cataggaatc gagccaggag ctggtgtagt tacggctgct gaaaacacga
     6541 tccaaaaata tgacaacttg aaagactgga aagtagagac atcttcttca ggagcaatga
     6601 ccgttgcact aggtcaagcg atcaaaaaac acgaaccaat tgtagttaca ggatggacac
     6661 ctcattggat gtttgccaaa tatgatctga aatacttaga agatcctgaa aacggaatgg
     6721 gaagcgaaga gcagatccat acgatggttc gaaaaggatt gaaagaagac cagccagaag
     6781 cttacaaagt attagacaat ttccattggt cagaaaaaga tatggaaaaa gttatgttgg
     6841 aaatcaacaa tggaaaagat ccacagcaag cagcaaaaga ctggatcaaa gagaaccaag
     6901 aattagtcga aagctggaaa aaataaaaag acagacacga aataaatgga gcttccaaaa
     6961 ttggatcaaa tatccaaatt tggaagctcc atgttcgtct ctatcttttt tgtctataaa
     7021 aaaagagaat gcgtagctca tcttcgaaac gtccaaagac gccgacaaac tattatgaat
     7081 ttatgggtgc gagtcaaaga gatcgatcgt aggtatgaaa gactaatgtg attaaagttt
     7141 attatgtacg aagtgcattc tcaaaaaata ttaaagaata tacatagttt atatacttaa
     7201 aagtgattaa attcttattc aggaattatt ttgctacgtt tcaagaagga attgtttttt
     7261 gaaatttaat gaatgttaga ataatagtct aaaaacatta atttaggggt gattagatta
     7321 tgaaacattt aaaaattttg tctattaaag agacacaact tatctatggg ggtaccactc
     7381 atagtggaaa atattatgga aatggagtgt attgcactaa aaataaatgt acggtcgatt
     7441 gggccaaggc aactacttgt attgcaggaa tgtctatagg tggtttttta ggtggagcaa
     7501 ttccagggaa gtgctaaaat gaaaaaaaat gctaagcaaa ttgttcatga attatataat
     7561 gatatatcta taagtaaaga tcctaaatat tctgatattc ttgaggtttt acaaaaggta
     7621 tatttaaaat tagaaaaaca aaaatatgaa ttagatcccg gtcctttaat aaatagattg
     7681 gtgaattatc tatattttac tgcttatact aataaaataa gattcactga atatcaagag
     7741 gaattaataa gaaatttgag tgaaattgga agaactgctg gaataaatgg tttatatcga
     7801 gcagattatg gagataaatc tcaattttaa tgatacttat aatattaaat atgtctcaa
//
LOCUS       DARBFD010000111         7859 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000111 DARBFD010000000
VERSION     DARBFD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.114"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..199)
                     /locus_tag="SCK08_002449"
     CDS             complement(<1..199)
                     /locus_tag="SCK08_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202291.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="SCK08_002450"
     CDS             complement(362..1996)
                     /locus_tag="SCK08_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1202292.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="SCK08_002451"
     CDS             complement(2000..3373)
                     /locus_tag="SCK08_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1202293.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="SCK08_002452"
     CDS             complement(3389..3628)
                     /locus_tag="SCK08_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202294.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="SCK08_002453"
     CDS             complement(3810..6485)
                     /locus_tag="SCK08_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEG1202295.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="SCK08_002454"
     CDS             complement(6668..7591)
                     /locus_tag="SCK08_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202296.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2294 a         1555 c         1195 g         2815 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ttagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgc
//
LOCUS       DARBFD010000112         7644 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000112 DARBFD010000000
VERSION     DARBFD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.115"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(60..788)
                     /locus_tag="SCK08_002455"
     CDS             complement(60..788)
                     /locus_tag="SCK08_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1202297.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(801..2081)
                     /locus_tag="SCK08_002456"
     CDS             complement(801..2081)
                     /locus_tag="SCK08_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1202298.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2222..2824)
                     /locus_tag="SCK08_002457"
     CDS             complement(2222..2824)
                     /locus_tag="SCK08_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1202299.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3069..3275
                     /locus_tag="SCK08_002458"
     CDS             3069..3275
                     /locus_tag="SCK08_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202300.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3587..4306
                     /locus_tag="SCK08_002459"
     CDS             3587..4306
                     /locus_tag="SCK08_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1202301.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4387..5070)
                     /locus_tag="SCK08_002460"
     CDS             complement(4387..5070)
                     /locus_tag="SCK08_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEG1202302.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5288..7474
                     /locus_tag="SCK08_002461"
     CDS             5288..7474
                     /locus_tag="SCK08_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1202303.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2735 a         1291 c         1439 g         2179 t
ORIGIN      
        1 aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt
       61 tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac
      121 gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat
      181 ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat
      241 tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa
      301 aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag
      361 atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg
      421 acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg
      481 ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa
      541 ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt
      601 tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt
      661 tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta
      721 tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg
      781 ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg
      841 ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata
      901 aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac
      961 tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc
     1021 ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca
     1081 ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg
     1141 attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt
     1201 aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc
     1261 ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat
     1321 ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca
     1381 tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc
     1441 atcctgactt gttgctctaa taactgatca gctaatttct tattagagat tttccaataa
     1501 taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct
     1561 aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat
     1621 aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct
     1681 aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga
     1741 aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgtcg
     1801 atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc
     1861 aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt
     1921 agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga
     1981 atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt
     2041 gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga
     2101 tttttaccaa attatagcac aattagcgta ttattttttt aatttttcag ggattttgaa
     2161 ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg
     2221 tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag
     2281 gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag
     2341 ctgagaaaaa taaagtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata
     2401 atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa
     2461 gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt
     2521 gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa
     2581 cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa
     2641 aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta
     2701 gttttccaag attattcaat aaaacttcta aggcaaattt tacttggaca tacaccatat
     2761 cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt
     2821 tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg
     2881 tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt
     2941 tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag
     3001 tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag
     3061 ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg
     3121 atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga
     3181 tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt
     3241 tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata
     3301 aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct
     3361 tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt
     3421 tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac
     3481 ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc
     3541 tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt
     3601 gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca
     3661 agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt
     3721 ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt
     3781 tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga
     3841 agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa
     3901 gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa
     3961 gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa
     4021 aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa
     4081 agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt
     4141 gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca
     4201 agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta
     4261 cactacaaat aacccgaaag caaagggaat ctttacaaat aaataatata aaaactaagc
     4321 aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca
     4381 ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt
     4441 gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg
     4501 atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg
     4561 gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc
     4621 tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc
     4681 aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg
     4741 ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa
     4801 caagcgccag aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag
     4861 aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc
     4921 gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc
     4981 atattggata taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat
     5041 tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg
     5101 tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc
     5161 atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac
     5221 gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat
     5281 caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa
     5341 caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga
     5401 agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga
     5461 aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga
     5521 acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg
     5581 gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt
     5641 agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa
     5701 acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat
     5761 cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat
     5821 acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat
     5881 acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt
     5941 agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa
     6001 atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct
     6061 cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca
     6121 aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga
     6181 ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg
     6241 gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta
     6301 tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa
     6361 aactcttttc ttattgacta attatcgttc tcgggcagct attgtccaag gagcaaatag
     6421 attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga
     6481 agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat
     6541 caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata
     6601 ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt
     6661 tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt
     6721 ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta
     6781 ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca
     6841 atacttttct cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc
     6901 caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga
     6961 ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa
     7021 ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt
     7081 cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg
     7141 cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag
     7201 taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta
     7261 taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtgac
     7321 gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct
     7381 gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa
     7441 agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg
     7501 tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt
     7561 cagcttattt tcgaagaagc tatttctgga acacagttta ttcggaaaag ggaggtgtga
     7621 caagaatttt gtcacacctc cctt
//
LOCUS       DARBFD010000113         7606 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000113 DARBFD010000000
VERSION     DARBFD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.116"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..179)
                     /locus_tag="SCK08_002462"
     CDS             complement(<1..179)
                     /locus_tag="SCK08_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG1202304.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="SCK08_002463"
     CDS             complement(192..1295)
                     /locus_tag="SCK08_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG1202305.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2341)
                     /locus_tag="SCK08_002464"
     CDS             complement(1385..2341)
                     /locus_tag="SCK08_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEG1202306.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="SCK08_002465"
     CDS             2622..4556
                     /locus_tag="SCK08_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1202307.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="SCK08_002466"
     CDS             5013..5429
                     /locus_tag="SCK08_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1202308.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="SCK08_002467"
     CDS             5443..6009
                     /locus_tag="SCK08_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEG1202309.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="SCK08_002468"
     CDS             6032..7018
                     /locus_tag="SCK08_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1202310.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7606)
                     /gene="scm"
                     /locus_tag="SCK08_002469"
     CDS             complement(7173..>7606)
                     /gene="scm"
                     /locus_tag="SCK08_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG1202311.1"
                     /translation="TTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDST
                     DTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGN
                     KSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2185 a         1456 c         1566 g         2399 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtgg
//
LOCUS       DARBFD010000114         7601 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000114 DARBFD010000000
VERSION     DARBFD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.117"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(943..1356)
                     /locus_tag="SCK08_002470"
     CDS             complement(943..1356)
                     /locus_tag="SCK08_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEG1202312.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="SCK08_002471"
     CDS             complement(1812..2297)
                     /locus_tag="SCK08_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202313.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="SCK08_002472"
     CDS             complement(2436..2651)
                     /locus_tag="SCK08_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEG1202314.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="SCK08_002473"
     CDS             2875..3675
                     /locus_tag="SCK08_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1202315.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="SCK08_002474"
     CDS             3694..5562
                     /locus_tag="SCK08_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1202316.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="SCK08_002475"
     CDS             5555..6046
                     /locus_tag="SCK08_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEG1202317.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="SCK08_002476"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="SCK08_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEG1202318.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="SCK08_002477"
     CDS             6606..7601
                     /locus_tag="SCK08_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEG1202319.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2709 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DARBFD010000115         7389 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000115 DARBFD010000000
VERSION     DARBFD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.118"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            123..515
                     /locus_tag="SCK08_002478"
     CDS             123..515
                     /locus_tag="SCK08_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202320.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="SCK08_002479"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="SCK08_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            857..1054
                     /locus_tag="SCK08_002480"
     CDS             857..1054
                     /locus_tag="SCK08_002480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202321.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="SCK08_002481"
     CDS             complement(1097..1672)
                     /locus_tag="SCK08_002481"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG1202322.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2353)
                     /locus_tag="SCK08_002482"
                     /pseudo
     CDS             complement(1827..2353)
                     /locus_tag="SCK08_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2810..4321
                     /gene="gadC"
                     /locus_tag="SCK08_002483"
     CDS             2810..4321
                     /gene="gadC"
                     /locus_tag="SCK08_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HEG1202323.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4334..5734
                     /locus_tag="SCK08_002484"
     CDS             4334..5734
                     /locus_tag="SCK08_002484"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEG1202324.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5899..7339)
                     /locus_tag="SCK08_002485"
                     /pseudo
     CDS             complement(5899..7339)
                     /locus_tag="SCK08_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2331 a         1229 c         1313 g         2516 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac
     2401 agaagggact tccttttttc tatttacaca aaatatttta cactctcaac aaaagtatct
     2461 tcgattaata cttgctcaag ttatgtgcag ctacgaaaaa gtaggtaccg aggtactttc
     2521 tttttcgcag ctttttttgg acttatgaga agtacattcg ctggaagaat acgatataaa
     2581 acgagctttt tcactaaaga tattaataga gtgaaagaaa caagcttttg ttgtcagttt
     2641 tttcactttt ctaaaaatga aaataaaaaa ttctggaagt ggagaagaat tttgcttgct
     2701 atactttgtc ttgtagaaaa gaggtggaca aaataatgca agctcaaata agcgattttc
     2761 cataaccttg gtaggaatga caaattaaat aggaaaggga agtaataata tgaataaaaa
     2821 aaagttatct ctattcggtt tttttgccct gacagcttcc atggttttga ctgtttatga
     2881 gtatccgacc tttgcaacat cacaattaca tttggttttc tttcttcttt tgggaggtat
     2941 tttatggttt ctaccagttg ctttatgtgc tgcagaaatg gcaacagttg aaggatggaa
     3001 agatggtggg attttcagtt gggttagtca aactttagga gaacgttttg gttttgcagc
     3061 tatttttttc caatggtttc aaattacagt aggttttatt acaatgatct acttcatact
     3121 aggagcgctg tcgtatgtaa ttaattttcc agagcttaat acagatccat ttatcaagtt
     3181 tattggtgta ttagtcattt tctgggtact gactttttca cagttgggcg ggaccaatcg
     3241 taccgctaaa attgctaaag caggatttgt cattggaatt gttattcctt cgatcattct
     3301 ctttggttta gcagcagcct actttatcgg cggtaatccg attcaaatgg ctattaatgc
     3361 gaaagctttt attcctgact tttctcagct ttcaacttta gtagtatttg tatcttttat
     3421 attggcttat atgggcgtag aagcttcagc atcacatatt aatgagctgg aaaatcccca
     3481 aagaaactac ccattggcaa tgtttttact agtgattttg gccatcgcct tagatgcaat
     3541 cggcggtttc tcagtagcag cggttattcc acaacatgac ttgtctctca gtgctggtgt
     3601 aatccaaaca tttgaaacgc ttattttaca ttttaacagt catttacatt ggttggtaaa
     3661 agtgattgct ctcatgattg cctgtggcgt tatgggtgaa gttagttctt gggtagttgg
     3721 tccatcacga gggatgtttg cagctgctca gcgaggtctg ttaccagttt tcttccggaa
     3781 aactaataaa caagacgttc cagttccgtt gattatgata caaggaatta tcgttactat
     3841 ttgggcagca gtcttaactt ttggtggagg aggcaacaat ttatcattct tagtagcaat
     3901 ctccttaact gttgtcattt atctcgtagg atatttacta tttttcctag gttactttgt
     3961 attagttttt aagaaaaaag acttaaaacg aacttacaat gtaccaggaa aaactgttgg
     4021 caaaacgatt atcggtggaa ttggttttat tttatcagtg ttcgcattgc ttatctcttt
     4081 tgtaccacca gcttcaattg cagctagtga gcaccgaaca taccaaatca tactacttat
     4141 tagctttgta gtgactgttc ttttaccttt tatcatttat gaagtacatg ataaaagtgc
     4201 tcacacaact attgaggaac ctactcacgt gaaagctgaa gacgttaatc ccgctgttta
     4261 tccagctgct agaggagagc accacgttac aaaaaatgat gaacatattt taaaacatta
     4321 ggaggaattc attatgttat acggaaaaga taatcaagaa gaaaaaaact atttggaacc
     4381 aatttttggc tctgcaagtg aggatgttga cttgcctaaa tataagttaa acaaagaatc
     4441 cattgaacca cgaattgctt atcaattagt acaagacgag atgttggatg aaggaaatgc
     4501 gcgattaaac ttagctactt tttgtcaaac gtatatggaa cctgaagcag tgaaattgat
     4561 gacccaaacg ttagaaaaaa atgcaattga taaatcagaa tacccacgaa caacggaaat
     4621 tgaaaaccgc tgtgtaaata tgattgctga tttatggcat gctccaaata atgaaaaatt
     4681 catgggaact tcaacgatcg gctcttcaga agcctgcatg ctgggtggta tggccatgaa
     4741 atttgcttgg cgtaaacgtg ctgaaaaatt aggtcttgat attcaagcaa aaaaacctaa
     4801 cctggtgatc tcttctggtt accaagtttg ttgggaaaaa ttctgtgtat attgggatgt
     4861 ggaactgaga gaagtcccaa tggatgaaaa acatatgtca attaatctag atactgtcat
     4921 ggattatgtg gatgagtaca caattggtat tgtaggtatt atgggtatta cttacactgg
     4981 tcgttatgat gatatcaagg gtctgaatga tttagttgaa gctcacaata aacaaactga
     5041 ctataaagta tacattcatg ttgacgctgc atcgggtggc ttttatgcac catttactga
     5101 acctgatcta gtttgggatt ttcaattgaa aaatgttatc tcaattaatt cttcaggtca
     5161 caaatatggt ttggtatatc caggtgtggg ttgggtctta tggcgtgacc aacaatactt
     5221 accagaagaa ttagtattta aagttagtta cttaggtgga gaaatgccaa ctatggctat
     5281 caacttctct catagtgcag cacaactaat tgggcaatac tacaactttg tacgctatgg
     5341 ctttgatggt tatcgtgata ttcaccaaag aactcatgat gttgctgttt atttagccaa
     5401 agagatcgaa aaaactggta tttttgaaat cattaatgat ggatcagaat tgccagttgt
     5461 gtgctataag ctgaaagaag atcccaatcg cgaatggaca ctatatgatt tatctgatcg
     5521 tctgttaatg aagggatggc aagtcccagc ctacccatta cctaaagact tggatcaatt
     5581 aattattcaa cgcttagttg ttcgagcaga ctttggaatg aacatggctg gtgattatgt
     5641 acaagatatg aaccaagcaa ttgaagagtt gaataaagct catattgttt atcataaaaa
     5701 acaggatgtg aaaacgtacg gctttactca ctaaaattct tcagagaaat ataaaagaag
     5761 ttaattaact aataaaagga gttgtgatta ccacacagct ccttaaatct atttgaataa
     5821 atatatgata ttcatttgtt acactctcaa tcttagctgt tgttctattc atcattactc
     5881 ttattgtaag tagttcagtc aaatttgctt gttcaattga tcgataacag tatgaattgt
     5941 ttgaaaagta atcggatata taattgtttt ctcactacct aagccttctt ggtataaaga
     6001 actatcgcaa agtataatcc gctgagtaca aaagaaagtg tcgtctacta tttctctaag
     6061 ttgtttacaa atgatattac taatagaaaa gtgttctccc aaaatgtctt ctaactgttg
     6121 tcgatagtat aaatacctaa agtctgacgg agctactatg aacacttcaa ttggagattt
     6181 ccgtttatgc tcatttaaaa gacttaaact aatggttagt ttacgaatag aattctcatt
     6241 ccaacgcaga aataaattgt tttgttttcg ccattgttca agaatttcat gaacttctaa
     6301 gtacaaagcg cgttgctctt catttaacaa gtaagtctta tcaggttgaa atatctgaat
     6361 atcagcccaa agtgagcgga taaacgggag aagtgcttta tcaaaaagta tctgattttc
     6421 ttcagatata ttcagagata agataagttt ttgactgagc tcaatcacca aagggttatc
     6481 tgctacatgg ttttgataaa caacgttcaa gtctttttgt aacaattcaa agttatcgca
     6541 tagataattt ctagagttga atagaaagta gatataaagg cattcatctt cgtcatgaca
     6601 taaagagaaa gaaagttgct ctaatccttt ctgtattagc tgaaacaagg gagtatgtcg
     6661 ggcaactctt ttttcttcct gactaaaata tattgggtta gtcttagatc tctgcaaacc
     6721 gatagcgata cctaataaaa taaattgttt ttcaactgta gggtaacttc ttgaaaaaaa
     6781 atgtttttct acataatcaa tcaattgttg accgctttga ataattgatt tatttagctg
     6841 gatcaactct gtccgatttg tctcatagat tatcgttagt aacaaacttc gataatcctt
     6901 ttcaggaatt tcaaaatggc catcagcatt taagtactgc atttgtttaa aaaaattaat
     6961 caactcattt cgtaagttgt aaactttgct taacgaaatg aaggtagtat cagctatatt
     7021 actccaattg tgttcccctg ataaataatg tcctagtaca ttgaaaaagt cagagcgctg
     7081 atagatgagt tgagtgagtg ccaacctcga aaacgaagag tcgaatgcga ctgtaaagct
     7141 acttctagat ttctccactg aaataatttg ctgatggagt atgtcggtaa tgcattccaa
     7201 gtcgttagtg acagacagtg agtgggcaaa cgttcaattg atctgctatt tcgcgaatct
     7261 gaatggtccc tttttcgtgt aattgctcac aaatgtaaac ttgacgaaaa atacttttct
     7321 ccagataggt ttccaacata gtactcctcc tagtcatttt atacataact attataactc
     7381 taccaatcg
//
LOCUS       DARBFD010000116         7388 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000116 DARBFD010000000
VERSION     DARBFD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.119"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..635)
                     /locus_tag="SCK08_002486"
     CDS             complement(<1..635)
                     /locus_tag="SCK08_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1202325.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLI"
     gene            complement(635..1771)
                     /locus_tag="SCK08_002487"
     CDS             complement(635..1771)
                     /locus_tag="SCK08_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1202326.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1803..3254)
                     /locus_tag="SCK08_002488"
     CDS             complement(1803..3254)
                     /locus_tag="SCK08_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1202327.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3298..4062)
                     /locus_tag="SCK08_002489"
     CDS             complement(3298..4062)
                     /locus_tag="SCK08_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEG1202328.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4090..4788)
                     /locus_tag="SCK08_002490"
     CDS             complement(4090..4788)
                     /locus_tag="SCK08_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEG1202329.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4800..5579)
                     /locus_tag="SCK08_002491"
     CDS             complement(4800..5579)
                     /locus_tag="SCK08_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEG1202330.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5595..6539)
                     /locus_tag="SCK08_002492"
     CDS             complement(5595..6539)
                     /locus_tag="SCK08_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1202331.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6585..7364)
                     /locus_tag="SCK08_002493"
     CDS             complement(6585..7364)
                     /locus_tag="SCK08_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202332.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2037 a         1586 c         1151 g         2614 t
ORIGIN      
        1 tcaatcaaaa aacgatgatt tttagaatat gaaattcttc caatatgtcc cattacaaac
       61 gtctgacttg aaattcctaa tttcattcgt acatcatttc taatactttc atcataataa
      121 aattttttag catccactgc atttggaatc aaagtgaatt tatcatttcg aaacaaaaaa
      181 tcacctgcta cttttccaca tgaaaattca tgtgtcaaaa cagacgttaa cttacatcga
      241 taatattgct ttaaaagata tttaaaatct ctatctatcg atgtattatg gctatgtgct
      301 atccgtatcg gaatcccagc tttttttgca gcttttaaag gtaaataact catcgcatca
      361 atatgagaat gaacaatctc atattctgga tgatccttaa aaaattgttc catccattta
      421 aaatatttag gataattttg tggataaagt ctcggtgcat aatagacctt tcctccaaga
      481 cttattattt cctcatcata atcactttta tatggacgat gtgtcaaaaa atcaaactga
      541 attttagttc gatctatatt cctataatag ttcattagca tcgtctccaa ccctgcacga
      601 tgcatatcat tcacacactg taatattcga atcattatat atttccccta tacaaattta
      661 atgtttcttg tacgatttta tcccaattat atttactaca aataaactca gtagcttgat
      721 tcttcaaaac tttaaccatt tccgattgat tgcatgcctc ttctaatctt atctttaaat
      781 cacttacatc cgatttctta aaaatcatag ccttgtcttc tactacttcc gtacactctg
      841 agatatttga aactatacaa cagttcccat aactcattgc ttctaacaaa cttaatggca
      901 ttccttctaa atcacttgga agtgtataga tatacgcatt actatataat tcttctaatt
      961 cctgtccttg tacaaatcca gtaaatataa ttcgctcatc accttttgct aattccttta
     1021 gttcatttgc aaattcatct gtatcagagc ttcctccggc tattataagt tttttatctg
     1081 tctgaacatc tttaaatgcc tcaattagat accgaattcc tttttcggga accaatcgcc
     1141 ccaaaaacaa aatataagaa tcttttgtta atccatactt ttctgtaatc aatcctgctg
     1201 attttatttc aattttttta acgccatttg gtataaacct tgtttttctt ccatatatat
     1261 cttcaaaata attttggacg ctttcactaa gtacaattat ttcatctgca aattttacag
     1321 cacacttttc ccctaacatg atgtatgtac tagcaagctt gttccatttt tctctttgat
     1381 gatctagacc atgaattgtt gcaacacatc ttttcccaaa aagttttggc aaccacaaca
     1441 tagcacatgg tccttctgca tgaaaatgaa caacatcata ttttccgaat gctgctctaa
     1501 ttccaccaaa tactgaagaa ctcattgctg caattccttt tatatttaat gtaaaaatag
     1561 tctttaattt tacaccttta tattctttat ttttatgctg gtcaaattcc tttccgctaa
     1621 catgatgacc acttcgatta tagcaagtca cttcatgccc aagtgcaacc attcgcgttg
     1681 ccaattcttc taccactatc tcaattccac cttctcttga tggaattctt ttatgaccta
     1741 gcatacagat ttttaatttt tttcttgcca tcacgtttta ctccttctat agtatcttta
     1801 tcttacatgg aaccgtttct atgaagcaca gttttgactg tcctaaacaa aattctaaaa
     1861 tctaatccca tactccaatt cctgatatat tctgcatcaa gttttacaac ctcttcgaag
     1921 tctgtgatat cacttctacc actcacctgc cacattccag taatcccagg tttcatcgca
     1981 attctcgcac gatgatgaaa atcatacttc tcatattctg acttcaagca aggtctaaac
     2041 cccaccaggc tcatttttcc cattaggaca ttataaaatt gtggaaactc atccaatgat
     2101 gtagatcgaa taaattgtcc aattcctgtc ttctttgttc catcaggaag tattttattg
     2161 ccaatgattc ttgggtcaaa atccatctta aacatgagat tattactcat cttattttct
     2221 ttcatcaact ctgctttgcg ttcttctgca tccatataca tactacggaa cttatacatc
     2281 ttaaatgtct taccattttg accaatacgt tcttgtgcaa agaaaatcgg ccctggtgat
     2341 ttgatgtaaa taactggtgc tacgaagata aaaataacac ctgttgcaat acatccaata
     2401 attccagcaa gaatgtcaat cgttctcttt ataaatgctt gcctcatcgt cataacatta
     2461 atactagatg taataacagt ataatcacca actcggccaa taaattgttt tccacgtgta
     2521 ctatcagaca cttttgtaag attcatatgt acagcaagtc ccatttccga acatctggcg
     2581 attaattctt tgggatatgg aacactttcg tcaacattga tgaatacttc atctacccaa
     2641 ttctgtaaaa gatattccgc tgcattatca gcatttgcaa ctactgggac acctccgatg
     2701 gtagttccat acatatctct gtctactaca atcacaccac taagtttata catgccataa
     2761 ttcttctctt taagattacg aactacattt tttgcaatct tttgagatgt tacaataact
     2821 aacgatacat cttcaccgtt tttcatgcgg tgatgcaaca accctttcca aagtatacga
     2881 attatatatg ttaataatcc atacaccacc cccattataa ataaagaaac tctggaataa
     2941 tcatttccat cctgcattgt aataaggtat aaactgcaca caagtaacag cataaaagat
     3001 tgctttatag aagctacagc ctctttataa tatcctcttt ttaaaacatt tttatatgcc
     3061 tcaaataaaa atataatcgt aagattctca agttctataa aaattgccat gtggcgataa
     3121 ataggaatca catacggatt ccagtcaccc tgtcgcatta catatgatat ataaaaagca
     3181 agctggacac aaaataagtc taataaaata aaatcaatat gtttagacca accacttgag
     3241 ttttttctat acatctctca ctctctcctc atttcctttt tatctacgaa taaagaacta
     3301 aaataaacca aacttcttct tttttacctc ttcatagatt ggataattga tctcatcccc
     3361 gttgatcaga tcctttgcga cttgtttcat ctgtactact ttttccttac caaactcctt
     3421 ggcaatctgc tcatagcact cttttaagta aaaggtgcgc ttagagaccc cgtgtgcatc
     3481 agaagcagcc atctgcacga gattatgttt gaccattgtt ttggctgttt tctggatatc
     3541 tttgccaaat acaccaacat aacttggcgc agtaagttgg gccaaacatc ccatatctag
     3601 gaacggaagc aaatgatttg ggtcttctcg aaaatgcgcg ttgcgttctg gatggacgat
     3661 cactggtatc tttcctagtt ctaacaactc aaaaaaaagc cgttctgcat ataacggcac
     3721 ttccagtgtc ggaaattcga tcagaagata tgtatcatct aaatctgtaa ataaaatttc
     3781 atctcggtgg atgtcttcga tgagcgttcc ggtaatacgt acttcttgtc cttccaataa
     3841 cgtcaaaggc aactgtcttt tctctaattc ggcttgtaac gaagcgacta gagaaatgac
     3901 ttgtgatttt tcattgctgt attttccatt attgtggtgt ggtgtgcata aaatatgcgt
     3961 aatgccttgc tgaatcgctt tttcagccat tgcaatactt gcttctaagt tttctgcccc
     4021 gtcatcgatt cctggtagga tatggcaatg tagatcgatc atttctcttg tctcctctct
     4081 tcttattcct taatttccgt agtaataacc agcatctttg ctgtgttctg ctccgttgta
     4141 tacgacacct aagactcgtg cttgtaccat atctaacagt tcttttgctt tagtcaatga
     4201 ttcttttcgt gacgtatttt cccgtacgac caataaggtg ccatccgttt ttgaagacat
     4261 gatctgtgca tctgttacgg caacgactgg cggcatgtcg aagatcacta catcaaatag
     4321 ttgtcttgct tctgctaaaa tctgatccat tcttggcgaa cttagtagtt ctgatggatt
     4381 tggtggttta ggtccacttg gcagaatcga caaattgtcg actggtgttc gttggatctc
     4441 atctgctaca cttcctgatg aactaagaac agtacttaat ccgcttgcgt tgttcaactt
     4501 gaacgtttta tagatgacgg gcttacgtaa gtctgcatcg actagcagca cccgttgacc
     4561 agatttggca aaaacaacgg caatgtttgc tgcagtggtc gatttccctt ctccaggtcc
     4621 tgatgaagtc acaacaatcg ttttgatttg ctgaccagct gcggaggcaa actggatatt
     4681 cgtccgaatc gtacgatatt gttcagcgat tggtgaagag ggattggtga gtgtgaccaa
     4741 actgactgca tgagtttggg ctgctttttg tttctgtgtt cgtgccattt tctttcctcc
     4801 tagactcttg agcggcttct gcgtgatggc gctgttttat cgtttgtagc ttttggttgt
     4861 gtctgagctg gtcctttttt acggatacca gcattcaatt ctttcgctga catttctgga
     4921 actgttccta agattgggaa tcctaatgta tctgtcacat atttttcatc tttcactgtc
     4981 cgatcgagta attccaataa gaatgccaga cctacaccta ccatcattcc taacattaag
     5041 ccagtcatta aattcaattt attatttggc gaaacaggtg tcgtattggc aactgcacct
     5101 gaaatgatcg agattttatc aacattcatc acatcttttg cgttctcttg gaaaacttgt
     5161 gaagttgtgt tagcgatctg ttgagcagcc actgcattcg tacttattgc ctgaatcgag
     5221 aacatttgtg aattttctgc ctgattaact gagatggcat ctttgatttg ttcagcagtc
     5281 attttcacat tgtcttctgt ttctaaacga tctttgactt cattgagcac taaatcactg
     5341 acgatcatat ctttatacgt attgatcatt tgcaggttcg tattgacatc attgacattt
     5401 gcagtgtctg attgtggcag tgtcacgatc aattgtgctt gtgaactgta ttttggtgtc
     5461 aagatgaaaa atgttacgat tcctgagatg gccaagccag caaacattga gatgatgatt
     5521 attaccaagt gtttgcgtaa tgtttcaaaa atttctttca agctgattgt ctcttccatt
     5581 ttttctccac tttcctattt attctctaga ttcatttgat cttttaattc ttttttcaca
     5641 cgatcaagtt cttgatcact gactttttga taggagatat tatcttgcat gaagccttct
     5701 ccttgcagct gatcttgctt aatcgtacca aatgcctccc gataatctaa agcaattttt
     5761 ttcatttcat caaagttcat gtctgttttc atattcgact ctagagcatt caaaacggat
     5821 tgatagttgc tgacaccgtt tagactcaat acttttttcg ctacgccttc tacgatcttt
     5881 ctttggcgcc cttgtctacc gtaatcacca tttgggtctt cataacgcat acgagaataa
     5941 gataatgcct gatccccgtt caatgtaatt ttaccgattg gaaagtcata gccatcttgt
     6001 gagaaagtga ggtcattatt gacttcgata ccaccaactg catcaacgag ttcctttagc
     6061 cctttcatat tgatagagac atagtgatcg attgggatgt ctacatattt ttgtacggta
     6121 tccatcgcca tggcagctcc gccgaatgcg taagcgtggt tgatcttgtc ttttgtgtca
     6181 cgacctacga tgtcgacata cgtatcacga ggaatgctga cgatcgttgt ctgtttgtct
     6241 tcaggactga ctgtggcaac catcatcgta tctgatcttc cttgatcgac ccgacctaaa
     6301 tctccagtat caatccccat caatagaaca gagaaagatt cttgtttgtc cagatcaact
     6361 ggtttcgttc gttctccttg ggttcgttcc actgattcgt aagttttctc aatagaatgt
     6421 gagagatcca gatataattt tgctcctact cctacaattg ctaatactaa tacgataaga
     6481 actgctaaaa taatcagaaa tatttttttc cctcttgatt ttcttcttcg tcttgccata
     6541 gtttcctccg tttgctaacc ttgcttgaat caacgtttta actgtcaata gcttatattg
     6601 ctatagccca tttgctcagc aacagtttgt gcttttgttg cgtatgctgc gcttattttt
     6661 tcattccgga tctgctctat ttgactgagc accgtcaaat attgatcgta agtagcggtg
     6721 gcagtcactt gaccatcctt taacatcgta tcgaataact gatctagttg cgtgacttgc
     6781 gcgatctgaa tatcagcgaa tcctagatat tgcataacaa ccgttttcca aggactatct
     6841 ttaaaaaagc tcatattttc acgtacacga gcgacatctg cacttgtcac cttttctttg
     6901 atgatgacat cgtctacagc ttgctgccag tttgatacat ttttagtaaa taaatcgttg
     6961 acagctgtct ggatcttcag ttttgattct gcactatcta actgtgtcac gattgctttc
     7021 ttctcatctt ggattgcaac agcttcttta ggcaattctg attctttgat gtcgtaatca
     7081 tctgctgaaa gtttgacaga ggatagctta tttgacaaac ttgtgattgt ttcttctgtt
     7141 atttcagggt ttagaaaaat tttttctttt gtactataca gcttgtctac ttcttttgat
     7201 aaagaagtca aggtgtcttt ttgattggtg atggcagatt ctgcataatt gatccgttgc
     7261 tgatgttgcc gctgattatt gatatggtat ccaaacaaac agatcgcaat agcacctgca
     7321 cctaccgcca cagcagtcaa tgacttgacg aatggctttt ccatagattc actcctaagt
     7381 attgatga
//
LOCUS       DARBFD010000117         6935 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000117 DARBFD010000000
VERSION     DARBFD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.120"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..1151)
                     /locus_tag="SCK08_002494"
                     /pseudo
     CDS             complement(1..1151)
                     /locus_tag="SCK08_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1394..1849
                     /gene="tnpA"
                     /locus_tag="SCK08_002495"
     CDS             1394..1849
                     /gene="tnpA"
                     /locus_tag="SCK08_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEG1202333.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2114..2212
                     /locus_tag="SCK08_002496"
                     /pseudo
     CDS             2114..2212
                     /locus_tag="SCK08_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2895..3713)
                     /locus_tag="SCK08_002497"
     CDS             complement(2895..3713)
                     /locus_tag="SCK08_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1202334.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3874..4563
                     /locus_tag="SCK08_002498"
     CDS             3874..4563
                     /locus_tag="SCK08_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1202335.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4577..6079
                     /locus_tag="SCK08_002499"
     CDS             4577..6079
                     /locus_tag="SCK08_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1202336.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6092..6568
                     /locus_tag="SCK08_002500"
     CDS             6092..6568
                     /locus_tag="SCK08_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1202337.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6665..6775)
                     /locus_tag="SCK08_002501"
                     /pseudo
     CDS             complement(6665..6775)
                     /locus_tag="SCK08_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2252 a         1092 c         1265 g         2326 t
ORIGIN      
        1 ccaaccttga tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc
       61 ttcctccaaa ccttccatta ccgctaatgc attgggcttt tcagagatac gataggataa
      121 aatctcacta ttgaacatat ccataaaagg atctagatac aatttctttt gacggataat
      181 gcctttggca tccacttcat ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg
      241 gcagatactg gtgtagaaac gacgatggat ccgatttttg gctactgctc cgacttgtcc
      301 tcgataagaa ttgtagcgcc gtgatttacg tgtgtatgtc gttactttta ttcctagctt
      361 cttgatcagg cgctgaactt tctttttatt gatagagaaa ccgcggtttc ttagttcatt
      421 ggtcatccgt aaacagccat aatccttgtg cttctcccga actttaagca tttccgcttc
      481 aatctcttga tctggatttt tacggtccaa tcgtttttgc cagtacataa agattgcctt
      541 agggaacttt aaagtttcta gaagctcaac taatttaaac ggtcctcgga ggcttgcgat
      601 gattcgtgcg attgcttcat tcgtcgttgt tgggcttcct gttttcgcag cttcctcaat
      661 tcttttaaaa aggcattttc aatttgaagg gaacggactc gtttctccaa ctcctcgatg
      721 cgttcacgtt ctttagacgt tgctttagtt gtagattttt tcttattttt gggttcattt
      781 ttcttagaca agataggtgg acatcctttc gtttttgaga gtccatcgat cccaccctca
      841 cggaattgac gcatccaact agcgatcaaa ttagggttgt tcatgttaag tgtgttggca
      901 acttcacgat aggacagctc acttgttaga tataactcta tcgcatccag cttaaattga
      961 acagaatatt ttttcttttg tcgctttctt actagaccct cttcaccaaa ttcttgatag
     1021 gcattgatcc agcgcttcac ttgtgatgga gccttaatgg aatatttctt tgccagataa
     1081 tcacttccgc ctttaccatc caagtaatca tgaacgatct ttaatttaag ttcaaaagta
     1141 tacttagcca taaaaaatac cgacctccaa aagttagatt ttttggtcta acttttgggg
     1201 gtcggtacaa aacttaaaag ttttagaccc ttttattttc ttaatgactt cagtcagttt
     1261 tgtgcgtaaa gcgtgcgaaa caagaaaacc gcgcgtttct acgcgtggaa gaagtatctt
     1321 tagcgataaa aactcctttc gatatactag tgagtggcta ccaaccgctc atcagatcga
     1381 aaggagtttt ttcatgtcaa atgacgataa aagtttagca tatactcgtt ggaattgtaa
     1441 gtatcatatt atttttacac caaaatatca acgaaaagta atctatgggc aattacgaaa
     1501 agatcttgga aaaatactta ggaagttgtg cgaaatgaaa gatgtggaaa tcatagaagc
     1561 acatgcaatg ctagatcata ttcacatgct tgttcgagta ccaccaaaaa tatgtatatc
     1621 cagttttatg gggtatctga aaggtcgtag tgcggtacta atccacgagc agcacgcaaa
     1681 tctcaaatat aaatacggaa acaaatcctt ttggtcgaag agatactatg tgagtacagt
     1741 cggattgaat caaaagacaa atgaaaaata catccgtgaa caagaagctg atgatcgcat
     1801 tcgatatatt ataagtaaaa gagagtacgt agaaccattt aaaaaataaa gagaagtaag
     1861 tgagtgaaga tgagcagttg gcggtctttt aaaaatcccc ttttaagggg cagcgagtgt
     1921 cgaccccttc tagggggatt aaaaatacac ccgtcctcac gggtgtatgg gttactgata
     1981 gacttgattt taactggtgt cagaaactta tgtccagaaa gggtgatgaa attttggcaa
     2041 tttacggcta ttcaagaatt agcacaagca accaagatta caaaacacaa attcaaacat
     2101 tagaaaatgc tggcgcagaa ataatcttcc tagaaaatca ctggattatc gaacaccttt
     2161 ggaagtattt ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag
     2221 agtatattta cttcagatgg taaggcaaca aaataaaata caataggcag atgaactaat
     2281 aaataaaatc tgtattaaat acagataaat taatataaaa atcaaaagga tcataaactt
     2341 ttaacaagtg atgaaatggg tggaaattga ttcttataga cagtttgtct cataacattg
     2401 aagtattttg caattttagc attgaaacac ttttttaatc ttcaacgttg ttcttataaa
     2461 caagatgatt gggaatcttt tgaattaaca ctgtatagct tagaacaccc tttatagaaa
     2521 acattcgctt agcaatctta ataattcctt gtgcccgatg tcgtttagat tcagtaggtt
     2581 cctgctcatc gatcatggaa aaaatttgaa ggatcagatc cttaataaat ttatttaaga
     2641 gaggatgatc cattgcttca tccatactcg ataaactagt gataatgagc tacacgtttt
     2701 tctttcaagt tgatcaatag cttccacgat aaaaatatct ttagataaaa tcaagaaccg
     2761 taggaactac agggatattt ggtcaataag agccaccgct attagtaaaa aataagctat
     2821 caagtatgct ctcatcccaa gaattgccta cttcaatcgt taaaaaccgt taggtttagc
     2881 taagtggggg tagttcactc aatactttta tcaataatag agcgcaaaac tttttctctt
     2941 aattcaactg aatcaatatt attgttttgt tcataaccat gaactaatag atcacaaata
     3001 taaagttgtg atatttttcc agctaaagat ccaccattaa gaaattcttc aatggctgtt
     3061 tgtaaaacta agtctgcaga ctttcctata ggtgaatgaa tatagttagt tatagcaata
     3121 atttttgcgc catttttttt agctattttc aatgaatcat aagtatcttt cgttttccca
     3181 gataaagaaa aaatgattac taaatcatta actgttaata aagaagcgac ttgggcttga
     3241 aaatgagggt ctagtacagc ttttgcttga actcctacgc gaagaaacat attttctaaa
     3301 tcaagactag tatttccact agatcccact ccaaatatat atatgttttt tgattgagtg
     3361 atcattttaa tggcttcatt caatttttct tcgtctaaaa gcctaatagt ggaatagaga
     3421 gcttctgtta aactattagc aatttcatca tagtacttta cactagtcgg atgttctttt
     3481 ttttgactaa agtcttcctt tgcaatttca atttttaaat cagaaaaacc agaaaaacca
     3541 attttttgac aaaagcgaat aatagtagca tcgccaactt ttgtgttttt cttaatatca
     3601 ctcatcgtac tataaataat agttttccca gaatttaaaa taaaatcagc gacttttttt
     3661 tcagacttcg ttaaaaatgg ataattttct ttaattaaat agatataact catacaaact
     3721 cctctacaat ttcaactttt ctccattata ctccaaaata ataaaaaaaa ctcctaaata
     3781 ctccattttt atctttactt ttatgaaagc ggatgctata ttgtttttgg agttgttatc
     3841 caaaaataat aaaaaattgg agtgtttagg ataatggata aaaagaggtt tttagatctt
     3901 atttcaaaag gtttgattgt ttcctgtcaa gcattacctg gtgaaccatt ttacaaagag
     3961 gaagaaagtt taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata
     4021 cgtgcaaatt ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt
     4081 ggaattatta aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa
     4141 gtagatgaat tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat
     4201 cgacatgatg gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat
     4261 cagttattta tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga
     4321 atagattttg taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg
     4381 ccagatatag atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga
     4441 aggattcata ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt
     4501 gtgggtggtg ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt
     4561 tagggggaga aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc
     4621 aattgctata ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga
     4681 gagtgctata aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat
     4741 tatgagttat gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat
     4801 tgctgtagga cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta
     4861 tctagtatat actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac
     4921 actagataca ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa
     4981 tcgttataga aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat
     5041 tccgattgtt tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc
     5101 accaattcaa aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg
     5161 aactttttta tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat
     5221 ttaccctttg ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat
     5281 tgctggagcc caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt
     5341 tacttatggt actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc
     5401 agcatgttat gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct
     5461 ttattttagc ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat
     5521 gtttttattt gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt
     5581 ttattttgct gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga
     5641 ctttttatta ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc
     5701 ttttggtatt gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt
     5761 taaggtagct attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa
     5821 caaaaataca tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga
     5881 aaatattgag tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga
     5941 gattattcaa aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg
     6001 tggcatccaa gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat
     6061 attaggaaaa gataattagg aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca
     6121 agaaactttt tatgcacctt gttccggaaa aatcatttcc atcacagaag taaatgatcc
     6181 tgtcttttca caaaaattac taggtgatgg ttttgccgtc atacctgatg gttcaaaaat
     6241 ttatgctcca ttggctggtc gagtaacaaa tatatttcct actaaacatg ctattaatat
     6301 aaaaagcaac tcaggcattg attatttgat tcatattgga attgatacag ttgaattaca
     6361 aggagagcct ttttcgattt gtgttcaaga aaatgatgag gttactcctg aaacctcgtt
     6421 ggttgatgtg gattttgaca aaataataaa atctggtaaa gatacttctg ttatagttgt
     6481 ttttccggag aaaaatcagt tcaatgattt aataatagat aatggtcgaa ttgctcatgg
     6541 gaattcatgt ggaaaaatca ctttttaaaa aagtgtatat aaaaagctat aaaataagct
     6601 aaataggata tttgtcaaat gaagttggtg aacagaaaga actgttttct ttctgttcat
     6661 ttttttattt tattagtaat gattccccat gccaaatcat gatccgtaat ttaaagttat
     6721 aaaagcttcg aaatccattg gcatttcgtt ggaatacctt gatatgatta ttcaaatata
     6781 aaatatagag agaagagaga gagggaagat atttataaag ggcaaagtta tgtttactca
     6841 acttaggaaa tagaaataga tcaatttata ttctcttgtc aatcgaatat gaaccttagc
     6901 actatatacc aaatatagta aaatgctgaa agatg
//
LOCUS       DARBFD010000118         6892 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000118 DARBFD010000000
VERSION     DARBFD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.121"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            106..249
                     /locus_tag="SCK08_002502"
                     /pseudo
     CDS             106..249
                     /locus_tag="SCK08_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            399..599
                     /locus_tag="SCK08_002503"
     CDS             399..599
                     /locus_tag="SCK08_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1202338.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(853..2532)
                     /locus_tag="SCK08_002504"
     CDS             complement(853..2532)
                     /locus_tag="SCK08_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1202339.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(2534..2746)
                     /locus_tag="SCK08_002505"
     CDS             complement(2534..2746)
                     /locus_tag="SCK08_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEG1202340.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            3139..4869
                     /gene="efrA"
                     /locus_tag="SCK08_002506"
     CDS             3139..4869
                     /gene="efrA"
                     /locus_tag="SCK08_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEG1202341.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            4866..6626
                     /gene="efrB"
                     /locus_tag="SCK08_002507"
     CDS             4866..6626
                     /gene="efrB"
                     /locus_tag="SCK08_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEG1202342.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            6721..>6892
                     /locus_tag="SCK08_002508"
     CDS             6721..>6892
                     /locus_tag="SCK08_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202343.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKV"
BASE COUNT         2107 a         1212 c         1447 g         2126 t
ORIGIN      
        1 gttttactat ttttgaaata agtaccttcc acttcaaata catgtattca aagtgaaagt
       61 attgatttaa atgattcttt tgtaatttac tcttgataat atgaaattcc atcgcagcag
      121 gatatgaatt ttaccaaaag gatacaaaag tgtggagaaa tgatggggat cactgtactg
      181 gaccacttga tcatcggacg caaacgatat tttagtttga gagaagaagg aatgatggaa
      241 gagaagtaag ttgctttctt gcaaaaaaaa tgaagcaagt gtacaattat attgtaattt
      301 tgtttgggaa tagaagttga aacatctgtt gcacctctat cgtacacgag caatgttaca
      361 atttaaatta gtgcttttgg cacgaggagg atattattat ggaaaacgga acagtaaaat
      421 ggttcaacgc tgaaaaagga tttggattta tctcacgtga agatggaagc gacgtattcg
      481 ttcacttctc agctatccaa ggtgacggat tcaaaacatt agaagaaggt caagcagtga
      541 ctttcgacgt tgaagaatca gaccgcggcc cacaagctgc aaacgttgta aaagcttaat
      601 tttatctgta aatcaaattg atggtagaca tcaattttta cgagagaaca aagcgagtaa
      661 caaaaatacc tgccacaaga gacaatttct cttgtggcag gtattttttt gtatgaaaaa
      721 gtaaataaat ttaagaggct atgaaagagc caccttgtat caaggaataa gaacaaacaa
      781 aaatggcttc tcatgttttt catttgtttt ctcttatatt caggacaagg tgctgatttc
      841 ataacctctt atttattctt catctggtgt cattaccatt ggcaaaatca ttggatgtct
      901 ttctgtacgt tcgaataaga aaggctgaag cgcgccagtc atcgcttctt tcaatttata
      961 ttctgaacag tcaggtccat tcaaacattc tcttaaagcg tggaataaga ttcgctgagc
     1021 ttcattgatc atttctcctg attctcgcat gtagacaaat ccacgagaga gaatatctgg
     1081 accagccaag atttcttttt tcttgatatc cactgtcgcg acagctaata caagaccttc
     1141 ttctgaaagc aggtgacggt cacgtaaaac gacattacca atatcaccga tcccgctacc
     1201 atcgatatac acatctccag ccacaaaatg cccagcagga cgcgcagaat cgctagttaa
     1261 cgctaaaaca tcgccatttt ccatgatgaa gcagttttct gcaggaacac ctacatcttg
     1321 tgctagagaa gcatgtactt tcaacatgcg gaattctccg tggacaggaa taaaatattt
     1381 tggcttcatc aaacgaagca ttaatttttg ttcttcttgt ccaccgtgac cggaagtatg
     1441 gatgttattg atttttccgt gaatcacttc tgcaccagct tcagacagca aattgatcaa
     1501 atgattgaca cttgttgtat taccaggaat aggtgaactt gaaaagatca ctgtatcgtc
     1561 tggctggatt tggatttgac ggtgagtacc attcgcgatg cggctcaacg cggccatcgc
     1621 ttctccttga gaacctgtac ataggatcat cgtttcattg gcaggcaagc gattgatttc
     1681 gtgtgcatct acaaatgtgc cgtctggaac tttgatatat cctaaacgtt gaccgttgac
     1741 gatggcattt tccatgcttc gtccgaaaac agcgatttta cgaccagttg ccacagcggc
     1801 atctgctgct tgctgcaaac ggaagatgtt agaagcaaag ctggcaaaaa tgatacgtcc
     1861 ttcgattttt gaaaagatct tcaaaataga atgcccaatc gttttttctg atttagtaaa
     1921 tgttggaatc tcagcatttg tactatcaga taaaaggcac aagactcctt cttccccgat
     1981 acgagccatt ttatgcaagt ttgctggttc gccaacagga gtgaaatcga atttgaagtc
     2041 tccagtcgct acgatatttc cagaaggtgt tttcaccacg atccctaatg catctgggat
     2101 actatgggtc gttctgaaaa agctgacaga agtctttcta aaacggatga tcgtatcttc
     2161 attgatctca tggagctctg catctcgaag caagccatgc tcatctaatt tgtttgtgat
     2221 caacgctaat gcgagaggac cagcatagat aggaatattt gcttgtttca gcaagtaggg
     2281 tattccgccg atgtggtctt cgtgcccgtg agtaatcacc aaggctttta ctttgtgtaa
     2341 gttttgtatg atgtagctgt agtcgggaat gacatagtca attccaagta aatcgtcttc
     2401 cgggaatttg atcccggcat caatcaaaat gatttcatct tggaattgta ccccatacgt
     2461 atttttcccg atttcaccta atccaccaat agcaaagacg cctgtctcgt tatttttgat
     2521 ggaaagtttc atattaaaac tccactaatg agaaatcagc attttctttt tcgtactcta
     2581 gatgacttcc ttctagaagt tgaacgaatt caatattgta tggggtattt tcttccacac
     2641 gttggcgagc gtctacatcg ctttttgcct caatgtaaag agattttgtt tcttcccgtt
     2701 tagggttgcg tacttttgtt tcttgataat aaactttata gatcattgtt ttgtctcctt
     2761 tatctgcgta aagcagtgat ttagctagtt gattttttta gacaacacaa aacgagggga
     2821 caagccacct ggtttttcat atgctttgac tagaatactg gattcgagtc aaagagacaa
     2881 gcctcgtttt tgcttatctg ttcggatatt ttttcaaccg taccttctgt ataaaaaata
     2941 tacaacaatt aattgttatt ctagcataaa acagcaaata agtatagaaa tcaactagat
     3001 tgaaagaaaa aatcaatgtt tatcatcccg ctataatcat aatgactttt ttatgagaga
     3061 agtgttctat taataggctc gttgtgctat catttttagt aatgaaaatg taacaaaaga
     3121 ttaaaaggag atgagaaagt gaaactaatg tggcattata ccatgcgtta taagaaattc
     3181 ttgtttttca atttcatatg cgtatttggt tttattttga ttgaattagg acttcctacg
     3241 attttagcac gtatgatcga tgtaggaatc cgcaatgatg attttgatta tgtgaagcaa
     3301 caagggatat ttatgatcgt tattactgta atcggtatta ttttgaatat cttgttaggt
     3361 tattttggtt cgaggattac gactaatatc gtagcagata ttcgagatga tttgttcgaa
     3421 aagatccaaa gttattcgca tcaggaatac gaaaatctag gcgtatcttc attgatcact
     3481 cgtacaacaa acgatgctta tcagatcatg ttgttcctgc aaaatatttt acgtatcggt
     3541 tttatggcac caatgatgtt cattgtcagt ttgtacatgg tcatgcgtac aagtgtgaca
     3601 ctcagtttgt atgtagtagg tgcattacct ttactattat tggcagtcgt agcgattgct
     3661 aaggtgtctg aaccgttatc taaaaagcaa caaaaaaact tagataaaat caacagtatc
     3721 ttgcgggaaa atctttcagg attgcgtgtg attcgtgcat tcgtcaatga aaagtttgaa
     3781 gaaaaacgtt ttgctgtagt caatgagaat tatgcttcta gttcaaaaag cttattccgt
     3841 ttgatggctg ttgcacaacc gggtttttat ttcttattta atatcgtaat ggtattgatc
     3901 atctggagcg gggcagtgca aatcgatgca ggcgagcttc gtgtaggaga tttgattgcg
     3961 tttatcgagt atatattcca tgccttgttc tcattcatgt tatttgctag tgtgttcatg
     4021 atgtatccaa gagcagctgt ttcagctgga cgtatccaag aagccctaga tgccaaacct
     4081 tcgattgtag aagatccaaa tggtatatct gatacaaaaa ctaaagggta tatcgaattt
     4141 caaaatgtaa catttgctta tccaggccat tcgcaagagc cagtgatacg aaatgtcagt
     4201 tttacagcct ctccaggaga gaccgttgct tttattggaa gtaccggtag cggaaaatct
     4261 acgttgatcc aattgatccc tcgcttttat gatgtgagtg aaggaaagat cttgatcgat
     4321 ggagtagacg tgagagatta tcggctaagc aagttgcgag ataagattgg gtttattccg
     4381 caaaaagcgc tgctatttac tggaacgatt gcagataatt tgcgatacgg gaaagaagat
     4441 gctacgcaag aagaaatgga acgtgcagct gagattgctc aagcttcaga attcatttct
     4501 cgaaaaccag atggatttaa tgaacattta tcagaaggtg gttccaattt ttcaggtgga
     4561 caaaaacaac gattggcaat agccagagcc atcatacgcc gtccggaaat ctatattttt
     4621 gatgacagct tttctgcatt agattatcaa acggatgcga agttacgtgc tcgtctgaaa
     4681 aaagaaacga cagaatctac tgtattgatc gtagcacaac gtgtgggaac gatcatgcat
     4741 gcagataaga tcatcgtatt aaatgaagga gaagtcgtcg gtatggggac gcatcgggag
     4801 ttgttggaaa aatgcccgat ttattatgat attgccgctt ctcaattatc aaaggaggaa
     4861 ttagcatgaa aaaaaccttt tcttccttaa aacgtttagc tcgatatatc agaccgtatc
     4921 gtgagacttt tcttttagtg atcctattta cagttctgac agtggctttc aacgtggctt
     4981 tgccatatgt tacaggactt cctactacag aaatcagccg taatattgcc aatggcgaat
     5041 caatcaattt ttcttatata ttcaaatgtc tcatttggat cacagttgtt ggaatagggt
     5101 attgtatttc gcaattactt tctggtgttt tgatgacaaa tgtcgttcaa agtgccatgc
     5161 atgatttgag aagagacata gaagaaaaaa tcaatcgtct tccggtttca tatttcgata
     5221 aaaaccagca aggaaatatt ttgtctcgtg tgacgaatga tgtagatgca gtcagcggtg
     5281 ctttacaaca agcttttatt ggtatcgtaa atgcgatatt gggaattacg ttggctgcag
     5341 caatgatgtt ttatatccag ccgatgatgg cgctgatctc tatgatcatg attccaacat
     5401 cgatctggat ctcaaaacga gtgatcaatg cttcgcagaa atattttcaa agcatgcaga
     5461 attctcttgg tgagctaaac ggctacgtcc aagaaaatat gacaggtttt agtgtattga
     5521 aagtatatgg acgcgaaaaa gaaacattgg aaggattcaa aaaagttaat cacagcttga
     5581 aatactttgg gtttcgtgca gcattcattt ctggcttgat gatgcctcta gttcaattaa
     5641 ctgcctatgc tacttatatt ggtatggctg ttctaggaag cttttatgtt attactgggg
     5701 cgatcgttgt cggtcaacta caagcattca tccaatatat ttggcaaatc agtcagccaa
     5761 tggggaacgt gacacaattg tcttcagttt tgcaaagtgc atcagcggca acgaaacgtg
     5821 tattcgagat tttagatgag ccagaagaaa aagaaaacaa agtagatgct tcactgccag
     5881 aaacgatttt aggagatgtg acattcgatc atgtcgattt tgcatatgat ccgaagaaac
     5941 cattaatcca agatttgaat tttgaagtga aagctggtca aaccgtagct gtggtcggtc
     6001 cgaccggtgc cggcaagacg acattgatca atctattgat gcgtttttat gacgtagaca
     6061 aaggagcaat aaggattgat ggaatcgaca ccaaatcaat gagtcgcagt gatgtgcgtt
     6121 cgctatttgg tatggtcttg caagatgcat ggctttatca aggaacgatt gcagataata
     6181 tccgcttcgg aaagcttgat gcgacagatt atgaagtagt tgatgctgcg aaaacagcaa
     6241 acgtagatca ttttatccga acgatgccag atggatatga tatggccatc aattctgagg
     6301 gagacaatgt gtctttagga caaaaacagt tgctgacgat tgcccgagca gtggtctccg
     6361 atcctaaaat cttgatttta gatgaagcaa caagttcagt agacacccgt ttagaagcgt
     6421 tgattcaaaa agcaatggat cgcgtgatgg agggacggac aagtttcgtc atcgctcacc
     6481 gtttatcgac aatccgtgat gcagatctga ttctagtgat ggatcaaggt cagatcattg
     6541 aaaaaggaac gcatgagagt ttattggcac aaggtggatt ttatgaaaaa ctgtataaca
     6601 gccaatttgc tgaggaagca gaataagtca tataagaata aaacagttta aaacttattt
     6661 tttgttctct attcttgata atggtaaagt aaagttatca agaaaagtga ggacacggag
     6721 atggaacagt ttttaaatga aaaaattact gaagccaaaa gagaagttga tcgtttaacg
     6781 cacgtctgtc agacgaagcc ttttgattca gctgattttt ttgaaaatga gtttcaaatc
     6841 gaacgcttgg cagcacaaat agaagtgtac gaattaattt taaaaaaagt aa
//
LOCUS       DARBFD010000119         6564 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000119 DARBFD010000000
VERSION     DARBFD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.122"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(7..1164)
                     /locus_tag="SCK08_002509"
     CDS             complement(7..1164)
                     /locus_tag="SCK08_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1202344.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="SCK08_002510"
     CDS             complement(1167..1523)
                     /locus_tag="SCK08_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202345.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="SCK08_002511"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="SCK08_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEG1202346.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="SCK08_002512"
     CDS             complement(2991..3830)
                     /locus_tag="SCK08_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1202347.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="SCK08_002513"
     CDS             4015..4902
                     /locus_tag="SCK08_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1202348.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="SCK08_002514"
     CDS             complement(5486..6181)
                     /locus_tag="SCK08_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEG1202349.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="SCK08_002515"
     CDS             complement(6165..6563)
                     /locus_tag="SCK08_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1202350.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2065 a         1300 c          966 g         2233 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catc
//
LOCUS       DARBFD010000120         6491 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000120 DARBFD010000000
VERSION     DARBFD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.123"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(182..1144)
                     /locus_tag="SCK08_002516"
     CDS             complement(182..1144)
                     /locus_tag="SCK08_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1202351.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="SCK08_002517"
     CDS             complement(1146..2585)
                     /locus_tag="SCK08_002517"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEG1202352.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="SCK08_002518"
     CDS             2770..4716
                     /locus_tag="SCK08_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEG1202353.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4980..5852
                     /locus_tag="SCK08_002519"
     CDS             4980..5852
                     /locus_tag="SCK08_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1202354.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6148..>6491
                     /locus_tag="SCK08_002520"
     CDS             6148..>6491
                     /locus_tag="SCK08_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1202355.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTG"
BASE COUNT         2074 a         1129 c         1156 g         2132 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacattttat
     4801 ttaatctttt catatatcaa ttgtatgaaa aattattaga attttaaaaa atgataaaaa
     4861 ttcaaaaaga aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta
     4921 aatatgatat atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta
     4981 tgctatacgg tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     5041 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt
     5101 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     5161 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     5221 aattggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     5281 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     5341 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     5401 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     5461 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     5521 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     5581 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     5641 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     5701 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     5761 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     5821 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     5881 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     5941 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aaattcttgc tcgaaagtta
     6001 tggaaatttc gatagccgta ggccacttgt attgtgcaat agttttttac acgccgtttg
     6061 tataattaag cgagacaaat aaaaaagcca tttatgttac actcaaatcg taatacccgc
     6121 taaagcatac aaggagagtg aacataaatg acttataccc atcttacacc aaacgagctt
     6181 gtaatgatag aagcatattt tcatcaagaa actccggttg ctatcgttgc gaagcagctt
     6241 aaacgtggac gccaaacaat ttacaatgtc tataactttc tcaaatgtgg tggaacagca
     6301 cttgaatgct ttgaacaata caaagaaaat aagcgacgtt gtgggagaac cgaaatcatt
     6361 tttcctgctg aggaaaaaga atacattgca aaaagatcaa ctgaaggttg gaccccagac
     6421 gtcattattg gccgtgcaga gcgaaccttc tcttgttcag taagtaccct ctatcgccgg
     6481 tttaagacgg g
//
LOCUS       DARBFD010000121         6464 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000121 DARBFD010000000
VERSION     DARBFD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.124"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(163..1242)
                     /locus_tag="SCK08_002521"
     CDS             complement(163..1242)
                     /locus_tag="SCK08_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1202356.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(1592..2440)
                     /locus_tag="SCK08_002522"
     CDS             complement(1592..2440)
                     /locus_tag="SCK08_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1202357.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            2439..3506
                     /gene="gnd"
                     /locus_tag="SCK08_002523"
     CDS             2439..3506
                     /gene="gnd"
                     /locus_tag="SCK08_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEG1202358.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            3508..4509
                     /locus_tag="SCK08_002524"
     CDS             3508..4509
                     /locus_tag="SCK08_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEG1202359.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            4506..4790
                     /locus_tag="SCK08_002525"
     CDS             4506..4790
                     /locus_tag="SCK08_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEG1202360.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            4823..5620
                     /locus_tag="SCK08_002526"
     CDS             4823..5620
                     /locus_tag="SCK08_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1202361.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            5634..>6464
                     /locus_tag="SCK08_002527"
     CDS             5634..>6464
                     /locus_tag="SCK08_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1202362.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
BASE COUNT         1899 a         1208 c         1458 g         1899 t
ORIGIN      
        1 catggtgacc cggttgctcg ttaccaccaa ccgtctaatt tttctaatcg agtgggccct
       61 tgacaatgag ctgaggcatg gtagtctccc gactggctgg ccccggccag ctttccagac
      121 acaagccatg ccgagggagg ctcgttgtca agggcccacg tgtcaagccg tcaaccgttc
      181 ctgcctaaat gcctggacga acgtgtcatg cggcgttgca tagcccaaaa tctttcgcgg
      241 gtaatcgttc atccaacgct gaatccgcaa gatttggttt tcgctcacgt tgccgattgg
      301 gctgcccttg gggatgaaac ggcgaatcag cttgtgttgg ttttcgctcg tcccgcgctc
      361 ccaggaagca taggggtggc tgaagtagat gtttgccaca tcctgcaagg catcgtgcag
      421 tcccgcgaac tccgagccgt tgtccgaggt aaccgtcttg aacagctggc cgaagtacgc
      481 accagcccgt tctctcagct gtcggaccgc ttggttgacc gattcggcat ccttcccggc
      541 cagcttcaga atgacctcga agcgtgtctg ccgttccacc aacgtcagca gcacggcatc
      601 cgacttggtc ttgtttccta cgaccgtgtc gacttcccaa tggccgaagg ttttccgggt
      661 accaaccgcc tccgggcgct cttcaatgga tttccctagc acccgtttat tcgggcggtt
      721 gccatgacgg gacgatttcg gtttgcggga cagtttttct agcaggtcca tattcttcgt
      781 tttcatgatg ccacggtcga tccactgata gagggtggtg gtcccaggga taatggccgg
      841 gtcgaacaat tcgtgttttc tggcaaagcc caccaccacg tccggggacc atttttccaa
      901 cagcatcatg tcgtccgccc attcaatgaa ggcgtccgtg tcggcccata agggccgtct
      961 gccgcagttc aagcggttct tctcgtaggc cgcctgcccg gcatcggcgt cgtagacgtc
     1021 atgataatag tcgtagacct tcccgttctg attctggcgg ttaagctgag taacggtccc
     1081 gcgtttgatt tcgttgttta tggtctgcgg cacgcagccc aggacatccg caacctgacg
     1141 gttggaatag ttttcctttt tcaggatggc gatttggcaa cgttctgagt aggttaggtg
     1201 ttttcccttg cgtgaaactg tgttatcatg gttttgcgtc atgtgaattc atccttgtct
     1261 attgagagtt gtggtaactt caatataaca tggaattcac gtggcgtttt tttatttcca
     1321 aatggctaag ttaattataa aatccgccaa caaaaataat aaaaaaaaga aaagataaat
     1381 tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa
     1441 tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg
     1501 taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt
     1561 ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc
     1621 gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata
     1681 gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg
     1741 tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt
     1801 ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg
     1861 tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag
     1921 aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca
     1981 aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga
     2041 cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact
     2101 tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct
     2161 cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga
     2221 tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa
     2281 aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta
     2341 ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt
     2401 tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata
     2461 ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga gtaggaaaaa
     2521 atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg
     2581 gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat
     2641 tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa
     2701 gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat
     2761 ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa
     2821 ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag
     2881 gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa
     2941 tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct
     3001 taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag
     3061 caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga
     3121 tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac
     3181 aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg
     3241 tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa
     3301 gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc
     3361 tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac
     3421 aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac
     3481 atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc
     3541 agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt
     3601 catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga
     3661 ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa
     3721 gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca
     3781 gcaaaagaac accagacaat cgtatctatc atacctgctt tagttgaacg aaatgctgta
     3841 gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca
     3901 agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac
     3961 aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt
     4021 aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa
     4081 agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt
     4141 ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag
     4201 atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg
     4261 atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa
     4321 gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat
     4381 atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc
     4441 gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata
     4501 aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt
     4561 tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa
     4621 gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt
     4681 tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg
     4741 aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg
     4801 cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg
     4861 agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa
     4921 ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat
     4981 aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga
     5041 tacactagca aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga
     5101 atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca
     5161 ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct
     5221 ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag
     5281 aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg
     5341 ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg
     5401 ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct
     5461 agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat
     5521 tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca
     5581 gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa
     5641 cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct
     5701 ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg
     5761 tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga
     5821 tttatatggg gggtttttct ccaggaggat caatgccagc tgaaccatct attgcagcag
     5881 ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg
     5941 cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt
     6001 ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat
     6061 caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg
     6121 ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg
     6181 ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg
     6241 tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg
     6301 ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg attattatcg
     6361 ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca
     6421 attcagcatc ttttgatgac gaagatgact atgatgacgg attt
//
LOCUS       DARBFD010000122         6262 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000122 DARBFD010000000
VERSION     DARBFD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.125"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(28..1506)
                     /locus_tag="SCK08_002528"
     CDS             complement(28..1506)
                     /locus_tag="SCK08_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1202363.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1804..2739)
                     /gene="murB"
                     /locus_tag="SCK08_002529"
     CDS             complement(1804..2739)
                     /gene="murB"
                     /locus_tag="SCK08_002529"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEG1202364.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2812..3732)
                     /locus_tag="SCK08_002530"
     CDS             complement(2812..3732)
                     /locus_tag="SCK08_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1202365.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3845..4597
                     /gene="xth"
                     /locus_tag="SCK08_002531"
     CDS             3845..4597
                     /gene="xth"
                     /locus_tag="SCK08_002531"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEG1202366.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5461..5994
                     /locus_tag="SCK08_002532"
     CDS             5461..5994
                     /locus_tag="SCK08_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEG1202367.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1883 a         1198 c         1046 g         2135 t
ORIGIN      
        1 gtgagacaaa gctgccatcc ataaatacta gctgattttt tcagttaaat gtctattttt
       61 ttctctgatg atggtatttc caaaatattc ccagtcatct ggagtcggtg gatttttcca
      121 aattatctgt tctttattaa atttttcaag taaacaatca gataaataaa tattcgtacg
      181 agaagtgatt cgactctcaa tcacaatatt gtaattttta aatcctttca cttgagaaat
      241 aatgtaatcg gtataagcag atccttgcga aaaatcaata cagacatgaa tcggtgcttc
      301 aaaatatgat accggacaaa taccgaccaa taaaaagaga taatcataaa ataagcgaat
      361 catgaaacgt tcatcttttc ccaagaaata gcgtttattc tcttgtatga ccttttggat
      421 tgctgtactg aatattggat acgtctcgaa gaaaaactgt agctgctttt tagacgtaaa
      481 gctagtcatc tcaaagtcga atatccaatt ttttcgattg acatgcatca tacccatacg
      541 aagttctttt cgtatatata gtctttcttc ttcagagatt tcaatcgaaa atggtaattg
      601 atcaatcaat tccactgaca ttttttgact aatcatgtgg atatcatgaa aatgagcttc
      661 cgcaagtcca aatgcgccat ctaattcaaa ttcttcttcc gtgatcaaaa acaacatgat
      721 ataagaaatc tcatcaatga tctgctcttt agggatatga agaaaagaaa tatgtgataa
      781 atccttgatg atcttcattc cttctgagct cttaaagtat tcatctgaaa agaaacgttt
      841 tccgattggt tgattcccat aggccctcaa caaactaata gtggttagca aattgaattt
      901 gattcgtttt gttgcagaca gttttaaatt gaacagatat tcaaaatatt gcacgatact
      961 ctttgcttca ttcatgacgc tttctctaaa tggcatagaa aaaccattaa agaccccgaa
     1021 atatacccca aataatacgt tccgtatgtt tatttcttcc ccaactaata tgtttttctt
     1081 taactttatt tgatgctgtt caagaaaaga gactaattct cttcttttta cataggctct
     1141 tgatatgctt aaaaaatgat tctctgcaaa tttttcgatc gttacttttg aatccacgat
     1201 ctccgttaac agtcgagcaa tttttgatcg ttgaaagtag attcttctta aatttttgat
     1261 atccgtcaaa ttcagacgtt cgacatggat ctctgcttct tctgaaattt ggatctctgg
     1321 ctttgttttg aattcagtaa attctttatt taattctatg atataatttc ttgttttaaa
     1381 tttagacaaa cctaatgttt catataactt aggcagcact acataattct ttttctcaaa
     1441 ttctaaatat ctagctagca gcagtttttc tctatcttcc tcttctataa ggtaatccaa
     1501 cttcatatcc tacacctcaa aaaatgcctc ataccaatta cttgatatgc agatgaaagt
     1561 tcatcttttc acctatactg attttatgat ggattatgct tgttttttca tcagttattc
     1621 tcatttttta tctttttttt gagaatattg caaaaaatgg aaaaatttat gaggaaatat
     1681 tctatatttt ttgttttatt gtgttaaaat gtacaagatt tgcgccattt gcgtaaacaa
     1741 ccacaaaaaa gagcaggacg gccaccgtcc tgctctttta cttattttaa gttccattac
     1801 ttattaattg gctaaacttc tgcttgtttc tgatattgct tgtttttctc caatgatccg
     1861 cacttctgtt tctaaagaca catcaaattt ttctttgatt acttcttgga tgtgcgcaat
     1921 caattcaata taatctgtcg ctgtcgcatg atcgatattg acgatgaatc cagcatgttt
     1981 ttcagagact tgtgcaccgc cccatttcag gccttgcaat cctgcttgtt ggatcagctg
     2041 acctgtatag tgtcctactg ggcgcttaaa gacacttccg catgaagggt attctaatgg
     2101 ctgttttgat tgacgcaatt cggtcaactc atccattctt gctttgattg cttcataatc
     2161 ccctgtttgc aaactgaatc gagcttccaa tacgatagca cgtaattctt ggactttact
     2221 gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt ttcaatgttc catcttcaag
     2281 caaaagatcc acttcagcaa aaacatcttt gatctctccg ccatacgcac ctgcattcat
     2341 ataaaccgct ccaccgacac ttcccgggat gccgcaagca aattcaaatc cagtcaatga
     2401 ttctgccaaa gctgcataag tcgtatcaat cagtgaggca cctgcttccg caatgaccat
     2461 cgttcctttg acatgaatct gtttcatttc agtcagcatg atgactacac cgcgaatccc
     2521 gccatcttga acgatcagat tgcttgcatt tcctaatacc atccaaggta tatcatggat
     2581 tcggcaataa tcgatgattt gctttacttc ttcttgtttt ttcgggaatg caaggacatc
     2641 agctggtcca cctgttttcg taaacgtata gttcatcaat ggttcatcaa acaatatagt
     2701 aatcgtggct aagctttcaa ctatttcttc tttattcact gaggattttc ctttcttttc
     2761 tcaaacgtcc tcattattct atcacgctta atacaatctt gccagtcttg ttcaaagttg
     2821 atgacggtag tgatctttaa gcacttcctc acataattca tgacttctta caacttcttt
     2881 ttgtttaagc aattctgttt ccgatttacc taaacggatc tgagaaaaga aggcctctac
     2941 cattggctca aacccgcgtt tgactagcgt gtttgtccag tcgccaaatt ctttcacttt
     3001 ttttccatca gcctcttgaa tgacaagctg ggtcagatcg tttacttcat aagtaccatt
     3061 tttacttgtt acttgatatt tttctatatt agcgccactt gctaaatcca tcgttaaaat
     3121 agccgtttga ttttgtgttt ctaattgtaa aatggcatat tccaaaaatt cctttgtttc
     3181 tcttatctgg tattttgttc tcaaaatggg ttcatctaat aaatacacag ctgtatcaat
     3241 aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt tcttttgccg ctattcgatt
     3301 tttttgcagg tggatcaacc gcttatctgg gatacgtttc aattcttcta ccataggagc
     3361 aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc ttatcagcta attcttgcaa
     3421 ttcacgaact tcatttagat ggacactcag tggtttgtca atataaacat ggataccatt
     3481 ttctagacat tttttggcta attcataatg tacttttgtt gctgcgtgga tcatacatgc
     3541 ttcgattttt tcttctaaaa gctcatcgat cgtttcatat agatgttgga acccgtactc
     3601 tttttgaatt ctttcttttg tttctttatt tcttgttgca aaataaaaat caagtgttcc
     3661 ttgttttttc gcataagttg gtaaatatgc tttttctgcg atattcccta caccgattac
     3721 accaatcttc atctactttc ctcctggatc atttagttac tttcatctta caccattttt
     3781 tactaaaatg aggtacaata gtatcgcaaa ggtaaggaaa ttaattcgaa ggagtgtgct
     3841 tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt gccatcgtaa ataaaaattt
     3901 tttagaagtt tttcatgagt tagacgccga ttttttctgt ctgcaggaaa cgaaactcca
     3961 agcaggacaa attgatttag atcttccagg ttattatcaa tattggaatt acgcggaacg
     4021 aaaaggctat tccggcacag cgatttttgc taaaaaacca gcattgaatg ccacttacgg
     4081 aatgggtatc gatatccatg atacagaagg ccgtctgatt actttggaat attccgattt
     4141 ctttcttgta acctgttaca cgccaaattc tcaaagcgaa ttaaaacgtt tagattaccg
     4201 attagaatgg gaagaagcct tttataatta tttggaaaat ctaaaaaaac aaaaaccagt
     4261 catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt gatttgaaaa actggaaaac
     4321 gaatcaaaaa aatgctggat tcactccaga agaaagagcg gctctttctc gactgttgga
     4381 taacgggttt atcgatacat tccgttattt ttatccaacc caggaaggtg tctactcttg
     4441 gtggaactac cgattcaatt caaggaaaaa caatgctggt tggcggatcg attacttttt
     4501 gacgagcaaa gatttagagc caggactagc tgatgcaaag atccatacgg acattatggg
     4561 cagtgatcat tgtccagtag aattagattt gaaataaaga ggttgtgaaa aagtcgttta
     4621 gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acattttgca taaatttggc
     4681 ataataccga gaaactggct tttgaacact gtttatcgag agattgtgaa aaagctgtcc
     4741 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catatttttc gtttggttgg
     4801 gcttattacc gcagatacaa gcttttgaac accgtttatt aagaggttgt gaaaaagctg
     4861 cactgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt ttcgtttggt
     4921 tgggcttatt accgcagatg caagcttttg aacaccgttt attaagaggt tgtgaaaaag
     4981 ctgcactgca tcaagtaata agaacagcca aacaaaaata gttcctcata tttttcgttt
     5041 ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattgaga agttgtgaaa
     5101 tcagcgtcct gacctgagta ttagagaaac aaatggcaaa atagttcctc atattttgct
     5161 attttttgag ctaataccga gggtcagctg attgaacacc gtttagtgga gaagttgtga
     5221 aaaagtcgcc tactttcgag tcgtaagaaa tttttttgaa aatggttcct cacaactttg
     5281 cataaatttg acttaatacc gagaagtcat gatccccatc taactccaaa tcacaaggaa
     5341 tgagttcaaa aactagcttt tctcaacaaa tattacataa aaaattctat gttttttgtt
     5401 aaatttattt gcttttttca aaagtatgtt acagtaattt cccaaggagg ttttacagaa
     5461 atgaattttg ttgccatgga ttttgaaact gccaatcatc agccttatag tgcttgttct
     5521 cttgcgctag tcatggtaaa aaatagccag atcgttgatg aattctatac attgatacag
     5581 cctgagactc cttttttctg gcgaaatgtc caaattcacg gaatccatca agaagacgta
     5641 cgcaatgcgc cgaaattccc agaagtctgg caaactatcc aaccatattt tcaaaaaaat
     5701 cgtttagtcg ttgcacataa tgctgctttt gatacaaagg tcttagcggg atgcttagat
     5761 tattatcatc ttgaacagcc taattatctt tctttatgta cagtgaaaag tagccgtcgc
     5821 ctgtttcctg aatttccaaa tcatcgattg aacactgtgt gcgaaaattt gaacatccaa
     5881 ttagatcatc accatgatgc attggaagat agtcgtgcat gtgcggaaat cctgctttat
     5941 caagaaaaaa aattcgggac agatccattg aaaaaacttg tgactataaa gtaaaagaat
     6001 acttatgaaa cacacaaaga ggtcgtgagc ctgccgtttc actccgagtc acaaggagca
     6061 atttcaaaaa atagcttctc atgtttatta aatattgtga ctggtgaccg aggagctggc
     6121 aggagaacac cgtttattgg agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa
     6181 cagccaaaca aaaatagctt ctcatatttt tcatttggtt ggacttatta ccgcagatgc
     6241 aagcttttgg acaccgttta cc
//
LOCUS       DARBFD010000123         6147 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000123 DARBFD010000000
VERSION     DARBFD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.126"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            102..2543
                     /locus_tag="SCK08_002533"
     CDS             102..2543
                     /locus_tag="SCK08_002533"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEG1202368.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="SCK08_002534"
     CDS             complement(2873..3577)
                     /locus_tag="SCK08_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202369.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="SCK08_002535"
     CDS             complement(3764..4009)
                     /locus_tag="SCK08_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202370.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="SCK08_002536"
     CDS             complement(4026..4877)
                     /locus_tag="SCK08_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1202371.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="SCK08_002537"
     CDS             5043..6047
                     /locus_tag="SCK08_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEG1202372.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1970 a         1064 c         1301 g         1812 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtg
//
LOCUS       DARBFD010000124         6002 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000124 DARBFD010000000
VERSION     DARBFD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.127"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SCK08_002538"
     CDS             <1..395
                     /locus_tag="SCK08_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202373.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="SCK08_002539"
     CDS             398..1228
                     /locus_tag="SCK08_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEG1202374.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="SCK08_002540"
     CDS             1244..2095
                     /locus_tag="SCK08_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1202375.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="SCK08_002541"
     CDS             2092..2451
                     /locus_tag="SCK08_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEG1202376.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="SCK08_002542"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="SCK08_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="SCK08_002543"
     CDS             2624..2929
                     /locus_tag="SCK08_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202377.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="SCK08_002544"
     CDS             2929..3240
                     /locus_tag="SCK08_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEG1202378.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="SCK08_002545"
     CDS             3237..3410
                     /locus_tag="SCK08_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202379.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="SCK08_002546"
     CDS             3407..3823
                     /locus_tag="SCK08_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG1202380.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="SCK08_002547"
     CDS             3823..4491
                     /locus_tag="SCK08_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG1202381.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="SCK08_002548"
     CDS             4488..4865
                     /locus_tag="SCK08_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202382.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="SCK08_002549"
     CDS             4862..5161
                     /locus_tag="SCK08_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202383.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="SCK08_002550"
     CDS             5151..5309
                     /locus_tag="SCK08_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202384.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="SCK08_002551"
     CDS             5403..5819
                     /locus_tag="SCK08_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG1202385.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DARBFD010000125         5982 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000125 DARBFD010000000
VERSION     DARBFD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.128"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(362..1144)
                     /locus_tag="SCK08_002552"
     CDS             complement(362..1144)
                     /locus_tag="SCK08_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202386.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1209..2045)
                     /locus_tag="SCK08_002553"
     CDS             complement(1209..2045)
                     /locus_tag="SCK08_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1202387.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2063..3472)
                     /locus_tag="SCK08_002554"
     CDS             complement(2063..3472)
                     /locus_tag="SCK08_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1202388.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3486..5309)
                     /locus_tag="SCK08_002555"
     CDS             complement(3486..5309)
                     /locus_tag="SCK08_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1202389.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1847 a         1288 c          960 g         1887 t
ORIGIN      
        1 tagactgatc gttaacttat tttttatatt tctacatagt tatctttaga accatttcag
       61 atacatatga aacaattaca tatgattttt tcataaaaaa acagtactgt ctagtagtat
      121 aaaatataca aatttcttaa aaattttttc actaaaatca ctacctgaaa cagtctaaaa
      181 cacttatata tcaacgttta atgaaactat ctccgtttca tttcagatac aaataaatat
      241 agtcgggctc caaatccgaa aaaaaatacc cccagtagct tatcctaagc tactggagat
      301 gtataagtac ggtcatataa caacggggta gggtaaataa atgaaaaaga tggaagaatc
      361 attattcaat aactttttta aatacttcgt tgtaatcagt tacctgtccg ttcttaaata
      421 ccacagaata atcttttttc ccattttcaa gtaaccgaag tggaatgtct ttaccaactt
      481 gctctgtcac tgcattactg atacctgtta gattagctaa taaatctgat aaagattgag
      541 catctgtaga aatctctgta tcagtattta tcgtgattcc tttatcttca tttacaagct
      601 tccaagtagg tggtatagct gtgtatgttt cgatgaatga atcaattctt tcatctatcg
      661 tcttcccagt atctacttct gtctcattac tcttagatga atcgattctt tcatccatcg
      721 tctgctccgt aactgcttct gtctcattat tctcaccgga aactctcttc acacccaaac
      781 ttatatattg tccatcttta gtgtgcatag ttaaaattcc cacgctttta ctaataaatt
      841 cactataacc agttctttca atagagactt caatgtattt cacactatca tactcaaaag
      901 aaaaatagac atgtaaaagc attttgttgt ttacatctac ataataatcc tttgactcta
      961 cttgagacct tcctatacgg aatacagctt tactgtctga tatctctata ccagcatcct
     1021 tattattaat ctgccaatct ccaagtagct cttctggcaa gctagtagtt tcttgtgaac
     1081 actttaccac ttcactggta gaggtcgaaa cattcacctc cgcactgtta gaagaatttt
     1141 ccaaggaatc aggagcctta gcacaaccaa caagtcttta tatattgtgc agccgatatt
     1201 ttcttcattc agtacaccct tgaaatatgc agtgttagat aaagcagttc gtcctgattt
     1261 gctgaggcac ccatctcttc ttgaacgtac tccttgattt tcaaggccgc ccgatactct
     1321 ttcggaaaac gctgcttcac tgaagccagc aaaaaatcat ccttctccgg caactcttcc
     1381 ttcttaatca ttcgggcaat aaaaaactgt aaatgcgtca caaatcttaa gtaaagcatc
     1441 gaatctttat caaccttttt ttcgggaaac agatatccta gcatagccaa gatatccttt
     1501 agtgctctta ccgagagcat ggtttcttcc atatttgctt gttcactttg ggcatttaca
     1561 atgtgaaacg caatgtttcc agcctcctct tctggcaact gaacattcat ttttttgttg
     1621 atcaattgga tagcatagca gccgatctca aattcttttg ggtaatactt ttgaatttca
     1681 atcagcaggc gattttgaaa aacaatgcct ttttgactgc gttcaatggc atgcttcaag
     1741 tgatcaacca aggtaaaaaa tagactttta ttcagcttaa agtcaaactt ggcgttggca
     1801 taatcgataa ttcgcttgac gcaaataatt agatcatccg gagcagcctc aaacagttta
     1861 atgtaatcct tttttagagc atcagaaagt gaatctaatt taaatacttt ttggattttg
     1921 tcagccggaa ccaaatcgcc tttctttagt tgaaatccaa tccctttccc catcaaaatt
     1981 acttctttgc cttgcttatc taagcaaaga attaaactgt tgtttaacgt tttaattact
     2041 ctcattagat tcaccgttct ctctaagcta agccatttct tgcgattact ttttgatacc
     2101 aaaagaagct ttcttttttt attcgtttta attctttaag attctcatcg gttcggtcaa
     2161 catatataaa accataacgc ttattgaagc cttgatgggt actaacaaca tcaatcgctg
     2221 accaaggtaa ataaccaaaa acatctacac catctgttaa agctgcttga atagccagaa
     2281 tatgtttggc tagataatca attcgatcct ggtcattaat tcgtctatct tcctcaacta
     2341 tgtcgggtgc cccatagcca ttttcagtaa ttaaaatagg taaatcatag cgatcgtaca
     2401 atttcctcaa tgttaatcgt agtccagtag ggtcaactac ccagccatat ggcgttttat
     2461 ctacataagg attttcagct gcccggtaga caccttcttc tcccaacatg atctgttgat
     2521 cccccgctct agcggaaaca tctgatccgt catttttgct ggcagcgatg gtggcagttg
     2581 aataataatt aatcgcaata aaatcgggtt taccggagga taaaattgcc tgatcctctg
     2641 cctcaatttt aggatataat ccacgatcat tcaaatagtt ttcaaacaaa taattgtatt
     2701 ttccccgaac tgctacatct aagaaagacc agcctctcaa tgtttcccaa ttatgggctg
     2761 caattgcatc ttctggactg gaggtagctt gatacatagc cgttaaattg attgctggac
     2821 cgatttttcc tttaagattc aactgatgat acaagtgaat tactctagct tgagctaata
     2881 aaacatggtg attaatttga aagagttctt tttttgaagg cagctctcca cttggtggca
     2941 aaccaatcgc tccaggatga aggatcatcg tgttttgctc attaattgtc agccaatagt
     3001 tcaccttgtc accatactca gtaagcaata cctgtacata gtccacaaaa taatcgatag
     3061 ttgctcgatt taaccaaccg ccctgctcct ctagaaaata aggtaaatcg aaatggtaca
     3121 tggtgacaat cggttcgatt ccctgattca ggcattcatc aattacttga tgataaaaag
     3181 aaattccctt ttgattggtt ttattatttt caatatccat aattcgcgtc caagcgatgg
     3241 aaaagcgaaa agcttttaag cctaattctt taaacagctg tatatcttct ttatagtgat
     3301 ggtaaaaatc cgaggcaact tcaaaattag ctaccccttc tgtatgctga tacttgtcaa
     3361 taatagacgg ttgtttccca tcctcctgat aggcaccctc tgcctggtag gcactaatgg
     3421 aggcacccca taaaaacttc tctggaaatg ctgaaagctg tttgtattgc acaaaaatcc
     3481 tctccttaac taatttgcat caatggttcg tgttccttta agcttccgct tgcttcacta
     3541 attcctgcgt aattttcact gttggtcact accacaatca ctgtatcatc gtagccagcg
     3601 tttctaatgg cagcgaaatc ggcgttgatt aataattgtc ctgctgacac ctgatctcct
     3661 gcttttacaa atacttcaaa gggttcccct tttaaattca cggtgtcgat ccccacatgg
     3721 atcaataatt caataccttc tttagaacgt agaccaatcg catgtttaga ttctaaaata
     3781 gtaatgactt ctccctcaac aggtgaataa atgctgtttt cttctggatg aatggctacg
     3841 ccctttccca atagtccctt agaaaagaca gcatcgttca cttcagaaat aggacacacg
     3901 gttcctttta ccggattttc aacaatgact gcttcggcta ttgccgtttg ctctgattca
     3961 tcagaagtat cgtcaaatcc aattagataa gcaatgataa aggttgcaac aaaggcgatt
     4021 acacacgtaa taattgcgtg aacaatattc attggatttt ctccaataaa ggctggtaag
     4081 gctgctaagc caggggaaac aaaggcatat ctgactaaac cgctcaagcc agcatacaaa
     4141 ccagcaattg caccaccaat catggaagca actaatggtt ttttcagctt caaggttact
     4201 ccatagagtg aaggctcagt aattcccatt aaagcagtga tcccagtgga cacagctagc
     4261 tgcttcaact ctttgttttt agttttcgcg gctacagcaa aggtagcacc tgattgagca
     4321 atattagaag ccagcattcc tggcccattg atcatttcag aacccgtact gctcaattgc
     4381 atagtagcga ttggcgtcat tgcccaagct gtacccgtaa ccaccaaaaa tggctgcaaa
     4441 ccacccatca aaaatggaat taaccaagct gctttgccgt taatccattc agcccccatt
     4501 gctataagat cgttcagcaa cgttccaaac ggtccaatga caaccaacgc taaaggcccc
     4561 ataattaaaa ataccagcat cggcttagta aagaatttaa taaacgaagg cgaaattcga
     4621 tccgcaaagg gctctacata ggacatgatc caaacagtaa agataatcgg taaaactgat
     4681 cccgcataat ctgcagctaa taccggaatt cctagaaaag aaacggcatc gccagtagaa
     4741 aaaagctgtg aaatatttgg atgaagcaaa atgccagcaa cagtaatcgc caaaattggg
     4801 ttgacatcaa actttatggc tgccccatag gctaatagta ctggcataaa ataaaaggct
     4861 gcatcagcaa tagtattcaa aatgatgtaa gtttgactac tgtctgatag tacaccagtc
     4921 aaagtcaaga cggccaaaat ggcttttacc atcccggcac ctgttatagc tggaatcatc
     4981 ggtgtaaagg tggtagaaat gacactaatc aatcttgaaa taatactttc ttgattgtct
     5041 gaagaggggc cctttttaat tgaagttccc acttcctttt gaatttcctt gaaggctgtt
     5101 tggacttcat tgccaatcac cacttggtat tgtccaccgc tatccaccac actaattaca
     5161 ccaagtgttt tttctaaggc tttcgtatcg gctttccttt tatctttgag ttcaaaacgc
     5221 agtctggtgg cacaatgggt taagctgacc acattttctt ttcccccaac ctgttgcaat
     5281 acatttcttc ctacttctct gtaatccatt attctctctc cttaaagcag taaaaaagac
     5341 caagtcaaac cacgccccaa tcaacattga agtgttagtt tgtcttggtc ttgcctgcat
     5401 aatcagtaac atgccaataa cgttattttg ctgtaatcca ataatagcac attatgatag
     5461 cgcaatcaac agggaattta attttttatc ttttcgtttg accgtatagg gtaatataac
     5521 agttagatca catcaactag ggtatatctg cgtatactta ctgctaaaat aagtatatct
     5581 aatattaact ataccagtaa aaccattcct acataaccag cgggaaattc ccaaaatcgt
     5641 gaaagcatca aaaatgcata taacggatca cacaacttca acaattctgc tcttgggaaa
     5701 aattgatgaa tttggtgagt aaaggatgga ataaacagca ttccaacaat ataggcaaca
     5761 atagtgatac ttgttttttt ggtaaaaatc agtaagcaat agccaatttc aatcattccg
     5821 agatataaag gtatcgatag aatgatttgg gctagtattc ttaaaaatta actagcaaat
     5881 gtttaaactg atttatttct aatctacatg tagtaagtat ttcaattcca ccaattatca
     5941 ataaaaatac caaacatatc cataataatt ttttcttttt ca
//
LOCUS       DARBFD010000126         5809 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000126 DARBFD010000000
VERSION     DARBFD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.130"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            450..1604
                     /locus_tag="SCK08_002556"
     CDS             450..1604
                     /locus_tag="SCK08_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEG1202390.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="SCK08_002557"
     CDS             1598..2020
                     /locus_tag="SCK08_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202391.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="SCK08_002558"
     CDS             3143..4075
                     /locus_tag="SCK08_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1202392.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="SCK08_002559"
     CDS             4092..4607
                     /locus_tag="SCK08_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1202393.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="SCK08_002560"
     CDS             4903..5184
                     /locus_tag="SCK08_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202394.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="SCK08_002561"
     CDS             5186..5602
                     /locus_tag="SCK08_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202395.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DARBFD010000127         5790 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000127 DARBFD010000000
VERSION     DARBFD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.131"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(99..1229)
                     /locus_tag="SCK08_002562"
     CDS             complement(99..1229)
                     /locus_tag="SCK08_002562"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEG1202396.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="SCK08_002563"
     CDS             complement(1877..3079)
                     /locus_tag="SCK08_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1202397.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="SCK08_002564"
     CDS             complement(3139..3342)
                     /locus_tag="SCK08_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEG1202398.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="SCK08_002565"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="SCK08_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="SCK08_002566"
     CDS             4879..5691
                     /locus_tag="SCK08_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1202399.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DARBFD010000128         5749 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000128 DARBFD010000000
VERSION     DARBFD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.132"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="SCK08_002567"
     CDS             complement(<1..1128)
                     /locus_tag="SCK08_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1202400.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="SCK08_002568"
     CDS             complement(1164..1874)
                     /locus_tag="SCK08_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1202401.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2757)
                     /locus_tag="SCK08_002569"
     CDS             complement(1942..2757)
                     /locus_tag="SCK08_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEG1202402.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="SCK08_002570"
     CDS             complement(2768..3736)
                     /locus_tag="SCK08_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1202403.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>5749
                     /locus_tag="SCK08_002571"
     CDS             4499..>5749
                     /locus_tag="SCK08_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1202404.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT         1772 a         1183 c          915 g         1879 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagtcttgc caaagaacat tcgtagcgga
     4801 tacctctgtt gctgaaaaac actgtttcat tagtcaaaaa gttcgttggt ctgtcattgc
     4861 tcgcttaaaa gagaatactt ccatgacgga aatcgctcgt cagaaaaata tttccacttc
     4921 ttcggtctat cgtgtcatga agcgattcta ccgcccattg aatcctttca aacagacttt
     4981 gccaaaggtc ttatgctttg atgaattcaa atcggttcga agtgttgcca gtgcaatgag
     5041 ttttatcatg atggacggac aaagccatgc attattggat attgtcgaaa atcgacgctt
     5101 gccttattta gaacgctatt tttctcggtt ttcattagcg actcgtgaag ccgttcaatt
     5161 gattgttatt gatatgtatg cgccttatgt ttcgttggtg aaaaagcttt tcccgaatgc
     5221 tcagttgatc atcgatcgtt tccacatcgt ccaacatatt ggcagaactt tcttgaatca
     5281 tcgagtgaaa gaaaccacgg tacgacttaa aggaaattcc cactctcaac gagggttagg
     5341 gaaaaaattg aagcgctatt ggaaactcct acaaaaagaa gaagcaaagt taaactatga
     5401 aaaacgggtt tggcgtgcca gttttaaaga ttatttgact gaaagtgaga ttgccgatcg
     5461 actgttagct gcttgtccca acttacgcca aggctatcag ttataccaag acattcttta
     5521 tgcggtaaag aaaagagatc agtcactatt tgaggattgt ttaactagag acattagcgg
     5581 tctgccagaa acctatacaa ccactttacg aacgtttaaa aagtttctgc cacagatcca
     5641 aaatgcgctt cactacagct attcaaacgg accattggaa tgcttaaaca accacatcaa
     5701 agtattaaaa cgcaatgcgt acggctttcg caacttttac aatttcaaa
//
LOCUS       DARBFD010000129         5746 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000129 DARBFD010000000
VERSION     DARBFD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.133"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(194..415)
                     /locus_tag="SCK08_002572"
     CDS             complement(194..415)
                     /locus_tag="SCK08_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202405.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(412..702)
                     /locus_tag="SCK08_002573"
     CDS             complement(412..702)
                     /locus_tag="SCK08_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202406.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(699..1883)
                     /locus_tag="SCK08_002574"
     CDS             complement(699..1883)
                     /locus_tag="SCK08_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1202407.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(2065..3468)
                     /locus_tag="SCK08_002575"
     CDS             complement(2065..3468)
                     /locus_tag="SCK08_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1202408.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3490..4263)
                     /locus_tag="SCK08_002576"
     CDS             complement(3490..4263)
                     /locus_tag="SCK08_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202409.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4273..4650)
                     /locus_tag="SCK08_002577"
     CDS             complement(4273..4650)
                     /locus_tag="SCK08_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1202410.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4671..4985)
                     /locus_tag="SCK08_002578"
     CDS             complement(4671..4985)
                     /locus_tag="SCK08_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1202411.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5185..>5746)
                     /locus_tag="SCK08_002579"
     CDS             complement(5185..>5746)
                     /locus_tag="SCK08_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1202412.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
BASE COUNT         1710 a         1127 c          953 g         1956 t
ORIGIN      
        1 tcagtctcat ctaaagttac ctctgatatt ttcattttag aaacaatctc gttacctcct
       61 tcataataat ttgaagagct ctttctttct aatgttttaa ttaaaagaga tttaaattga
      121 tcatttgtaa acatattcat aaatcctttc ataacaaaat atatacttcc tattttatca
      181 tgaaaaatat ctgttaagca ccaatgattc ggttaaataa gtttaataac acatctttca
      241 cgccagctgc attgaacaca agtccgacgg cgattagagc aataatcaag aagccaatga
      301 gttttgaaaa ttctcgttta aaacctaaaa acagtccaat gacaacaatc gctaacaata
      361 ccaatgactg tgcattatcc aagaaccagt tgtacaaatt ctgcccaaaa ttcatgtgat
      421 tgtcctcctt tccttaagat atttttctcg ataatagata tagcttttta cacgccttaa
      481 taattgaaaa ggggttaaat gctctccata gacggttact cgattggtat aattattata
      541 aaatctcacc ttgtaaaaat cataggtata gatggtatca ccagcttgcc gttttccctc
      601 aataatgaga gacgtatatt tccctgtatt ttgtatatac gcttgaaact caatgccaca
      661 ctgaaaccct aacttcttta attcttttgt taaaaggctc atgtaatcaa atcctccatg
      721 gctagatgtt gttgttcaat taatttctca tgtttttctg tcagtttggc ttcctgaatc
      781 atggtggaga taatggttgt accgttgaga acatctaaaa tgcttgcgat ttttaatgta
      841 ggggcgactt gatgatgtaa ccagttcaat gttcgttcaa aagaataagg ctctggctgt
      901 gtggttaggc gtaatgctcc acgattttta ccgataaacc attcccaacg ttcattagtt
      961 ttccaatcac tccgacgttt agtgtcgtct ttatctgcaa agcggatata gcgattgata
     1021 atttcaaagg ctgttttttc cgcctgttga tgtgccaata aatctttcat ggcttgggtg
     1081 gctcggtcat tttttaaccg aatttcaaac cgattcttta cttctgcgtc ttctaaggga
     1141 acatcatttt tcacgtattg ctcataatct ttctcatata tacagaaata gacttcactt
     1201 tttaatgagc caatataaag tgtgtttccc atgtatggtt tttcatcacg gtgaaccaat
     1261 tcaccactgc gataattttt aaagcttcga aagacggaaa tgcattcttc ctgtttacat
     1321 ttctttgcca aatctggaat attcaaaatc cctgttttat cgttgatagc taaatccaag
     1381 cgtttgaaaa tacctttatg ttccatgcaa tcttgaaaga aatcatacca gctccgatgt
     1441 tgagccaata gaaagttttc aaattgccga cagccttttc ctttcagttc taataaaaca
     1501 cctttactta catcatgaga gactaggaca acaatatcac cgaaacgata gtgttcggga
     1561 taagaataat agccaaagtc ttcgtgtagc atataatcca tattcaaacg taaaacctca
     1621 ccgataattt gagccacatc attggtaggg aatcgaatcc gtacatagtc gaataacatc
     1681 tccaatgggt tatcgggatt gaagcgttcc aaagcgttca gtaaatcttc tttcacctgt
     1741 tgagtaggga ctgccttgcc tgtttcaata tccgataggt aaggtctggt aatatgagaa
     1801 gcaaccgcca gtttattttg agaaacgcca taagccagtc gcttttcttt caattcctta
     1861 atccaaattt tttcgttttc cattcgcaaa cctccaatct aaaaaagatg taaactatga
     1921 cccttgagtt tacacctttc atattttccg caatcctttg taccatcaac attggttgat
     1981 gagaattgcc tgttttcagt tgtatttgtg cccccctgtt agatagaggg ggctaaagga
     2041 ttggcgtggc taacgccaca ccaatcaaag caagtctgaa cctgtacctt tcacttcgtg
     2101 cggtacaggc tcagattgct ttttttgaat gacttttcct atttctccta agaaatcgta
     2161 cctttttggt acaaggggtg tataaaactc actaatcaca ctttttcctg tatccacgta
     2221 acctcttccc ttgatgggtt ttaagaaaaa ttgtttttgt gtatcgcttc cgaacatcat
     2281 accaaagccc atttcagaca tacgacccaa agccacacgg aagttaaagt tgtcccgaat
     2341 cccatcacta aaatattttg cgtctggacg ttgacaggca agaattaaaa agaagcctgc
     2401 ttgtcgtcct aacattacaa tttgtttgag tttatgaata acggtggtac tttcacgacc
     2461 tagcatatcc atcattgcca catattcatc aaaaataaga aaattggcag acaaaccaag
     2521 ataagcataa ttttctcctg ttttatagtt agggtgttgt ttcatggttt cactgcgatc
     2581 aatcatatct tcgtaaaatt gattgataca tgccaacata tcttctttac ggtaatagac
     2641 attattcatg accgccccta agtctgccaa atcagcattt ttagggtcaa gaatataaag
     2701 ctttgcattt gtatgcagga gagattcaat caatgtgaga ataaaatagg attttccact
     2761 accagtgccc ccagcaatta acatgtgagg gagagaatcg aactcccaat agaccgattc
     2821 catcaatttg acacgtccat cttgagctac cacgtcttca atgccaattc gattgccaat
     2881 agtatcataa agtaggacat actctacata agaatctttc aacgtttttt ccactaattc
     2941 acaatacaat cctgtttcta gtttgttttc taggtgcaaa agctgatctt gatatttccc
     3001 taaggtgatt tctacatgaa tatggattag tccatctttc attcgataat atattttagg
     3061 aaaatggcgg attcgttgtt tggttttctt gctgggtaaa tctttaaaga atccctctga
     3121 ttgagtttct tcggcttcat accaatgatt ttctaaaatc atacgagcta acttttgacg
     3181 gtgctctaac tgttttatag aatctatacg atagtgataa taaagccagc tgataagtcc
     3241 gcatacaaaa agagcagtga gaatactgct ccaaaagtat ggcgtatgaa gttgatagaa
     3301 aaatgtatct aactgaactg cttgccagtt caccgattgt aacgctctcc aatgaaaagg
     3361 gagtaggacg actagaaaaa ctaccagcaa tgaaattagt acaaaatgat agaccaagtg
     3421 cttatcgttg gctttgattc gctctcctct gtcatgaagc aatggcataa aaatcctcct
     3481 ttccacctat caaaataatt gtgtattgta gatacgagcc tgtttatcaa aagttggttg
     3541 agaaatggct tcatatttct gataagcgat tcgcttttca aagcgtaaat ttttttcttt
     3601 cggataataa tgacgtcgat agagagtgtg tcctaacagt tttttcgctt cttttgtcaa
     3661 cggatagaga tagcgaaaca tgcgtccatt gattttctca atccctttgt attcgcaaaa
     3721 tgcctgggaa agccaatgct ttttctctac ctgttcaaag cgagcatttt cctctaataa
     3781 aaggcgagca cttcgagggt gtatcttttc ccatgactgc ttatcacgat agacacttgt
     3841 cttgaaatac ccgcagtaaa agaagttgga cgcttgatag acatagccac attttccctc
     3901 aattccatct gccagtgtat ataaaaaatg acagtccgta tgttctttca accacttaat
     3961 tagagctgaa agagcttgac tgccaaaata attggtttga ttcatttctg gaagaaaaca
     4021 catctttcca atttctaaat aatctgtggt ttgaagggaa ctgtcgggaa agagtttgcg
     4081 tatggtttgt aggggttgcg taccccagcc taattccaca acgcctaata atttttcttc
     4141 tgaaaaaatc cctagaaagt atttacacaa tctagggagt atctttgaat aatggtattg
     4201 ccgaataaaa gaaatggctt catccttcgg aatttctttc aaaatcaatg gtgtgtttgt
     4261 cataagcaac acctattttt cactttcgga cgcttctttt ttatatttct gatgaggttt
     4321 tgtctgtcct tttaatacaa gatcgtctgc tttcacgtac caatcaacgt ctgcccctcg
     4381 aaaagtagcc gtcgctacgg tatctgccac aggattgatt aattccactt ctgcgttgta
     4441 gtcaaattct ttcaaaggaa cagaagcggg gatactgact tgtatcatgc gtccttgtcc
     4501 ttttgatttt aagtcatagg tacgttcttt aatttcttcg gaaaccgtac catcttcatt
     4561 ctggatacga acctcacgac gcaaggcgga aaatttcaat gtgccaaatg tctggtcttt
     4621 ttcaatcaca ataccttctg ctaatctcat attttttcac ctttcctttc ttatgctttc
     4681 accatatcgt ctgcgtgcat aatgtaattg gtaaatcctc gattgccaat tttataacct
     4741 tctgcggtaa tatgtgggtt gatgagtttg actttttcgt cggattcaaa atgtttctcg
     4801 ccagcttcgg agggaagaat gacaacaata tcatctgctc gttgaatcgt tgagtataaa
     4861 ttgtaactac gagagagaat ggttggtctg ccatttattc gacgttgttc aatgttccct
     4921 tcaccagcat attctaattg tccaaatgtt ttttccatat tgggaatgac atatttaagt
     4981 tccatatttt tacctatcct ttctgttttt atgtttgaat gaatcttatt tgtgtgatgg
     5041 ttttaaaggg gggagagcga ccttttgatt cttgattttt tattttcata agttcacttc
     5101 ctttcaaaat tgggtaaaaa aatagacgct ccgtactgag cgtccgattc ctactatatt
     5161 taaaacaacg actacctagg tattttaaat attataatca aattctattt tatctaataa
     5221 ttgagtggga taaagcaagt ctcctttact cgtaaaataa cccacctctg agaaaagaaa
     5281 taaattattt tttgctcttg aacaaattac gtatagtagt tttctcgctg aactaattcc
     5341 tttatcagaa acattaaaaa tttcattcca atgaggaatt ttcccgttta gaagattaat
     5401 agcgataaca gtatcatact catttccttt aactttatgt gctgtagtaa gtgtgatacc
     5461 atctcgagat ttaaacatct tcttaaaact agatacacta ctagataatg aattattttt
     5521 tatccgatct tctacttcgt taataaaaaa tagatagtgc ttaaaaatag acgggtaatt
     5581 tttctcgtca atattaagat taacaaatat atgtttggta acaaatttgt ataaatcaac
     5641 accgttgtcg tctccactat acattctttt cacactgttt attagctgta ataaatctaa
     5701 gtcaataaaa taatctttaa cttctattcc aaattcattt tttaat
//
LOCUS       DARBFD010000130         5651 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000130 DARBFD010000000
VERSION     DARBFD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.134"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            165..1076
                     /locus_tag="SCK08_002580"
     CDS             165..1076
                     /locus_tag="SCK08_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEG1202413.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1563..1829
                     /locus_tag="SCK08_002581"
     CDS             1563..1829
                     /locus_tag="SCK08_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202414.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            1901..2242
                     /locus_tag="SCK08_002582"
     CDS             1901..2242
                     /locus_tag="SCK08_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202415.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2297..2716
                     /gene="ssb"
                     /locus_tag="SCK08_002583"
     CDS             2297..2716
                     /gene="ssb"
                     /locus_tag="SCK08_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1202416.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            2793..3089
                     /locus_tag="SCK08_002584"
     CDS             2793..3089
                     /locus_tag="SCK08_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202417.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3104..3535
                     /locus_tag="SCK08_002585"
     CDS             3104..3535
                     /locus_tag="SCK08_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG1202418.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            3717..4022
                     /locus_tag="SCK08_002586"
     CDS             3717..4022
                     /locus_tag="SCK08_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202419.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            4426..4914
                     /locus_tag="SCK08_002587"
     CDS             4426..4914
                     /locus_tag="SCK08_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1202420.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            4901..>5651
                     /locus_tag="SCK08_002588"
     CDS             4901..>5651
                     /locus_tag="SCK08_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1202421.1"
                     /translation="MEKIKQTFPLINKYWNKKKIFIFFITFLLSSLFSLIFPYLFSNV
                     IDLYLENELSQNIVRIVTILIFQLFLVLIKEVYGYIKTIYAFEVDLSMKIDFLKVLQK
                     KNLFFSQTKHSGEIQFRMFSDISTIVNTIFIFSVELPVIGVLVLVAVFIISKVNLIFL
                     IFSLIMMGAMVLQYNLFQLPLKKTIENTKSVNQSITGELNAHFDQLEIISAYNFENIS
                     ILKFDSRFKELFNVFKKQNKLLTLGSCLAQVI"
BASE COUNT         2104 a          761 c         1115 g         1671 t
ORIGIN      
        1 aaacttgcga gagagttgga gtaaaacaag tgtagaggaa acttgaagac ttgttcgaac
       61 acaaacttac aagagagggg agtaaagcag ttgcagagga atcaggaaga tttgttcgaa
      121 cagaaacttg caagaaagca ttattaaatt gaaggtgatg aaatatgaag tatttattgc
      181 atggctattt tattgattat tctactcgca atagtagtac tttagcgatt ctatgtttgc
      241 ttcatgaatt gagagtggtt actagaaaac agctatacaa actattgaat tttgacagat
      301 caattagtaa acaaggagta acgaatgctt tagtgagatt atatgaaaaa cagttaatcg
      361 atcgaaaaaa atatggaaca gaaatgtatt attatttgac gaaagaggga catcaaagca
      421 taggtgggta ttatacgttg ccgaaagttc ctgaatataa tttgaatcac catttacaaa
      481 ttaacaatta tttgattaaa ggaatggcat tggcaaaaga ccacccacat ttaaaacgga
      541 ttatttcaga aagacgtacc gtctatgaaa cgaaggattt aaatcctaag acaaaaggaa
      601 gaaaattttt tgtgccagat tttatttttc gttttcgaga caaagaaaat catgagatca
      661 attggtcctt tgaaattgag ctaacgttga aaacacgtcg tcgctaccgt gaagcgattt
      721 ttccgaaata tattcgtgag ttgcgaacac gtcctgaatc acatttaatt tatgtgacac
      781 ctagtggcgc aattcaagaa gaacttcatg cgtttaagcg tatttttttc aagcgcgaaa
      841 ctgaaaaaga tgcgagtcaa caagaattgt ttgagcggtt gcatattttt tcagctttag
      901 agtttgaaga gggattaaaa gcattattag agaatgatcc agcagtcaat tggcggacag
      961 tagaacgaga aaaaatttat ccgaatggcg ctgttcaaag tcgtttgaat ttatttgaag
     1021 aatcaactga aaatgaggaa aaaccaaaag tgaagtttac gcctgaagta ttgtaaaaga
     1081 gggtgttcgc ttcgctcttt ttttgtgcca gtttgctggc accgttcaga cgcaagcgtc
     1141 tgaaccggct cgtttttagg aaggactaac ttgtttgatt tatccgttgt caaagaagtg
     1201 gacgttcgag agtccctaat cgtatagcag tcattcggcg agtataacct aaaacataac
     1261 ctgccaattg ccaagggtaa aatgcacgct gcgcgccctt ggcaatcgtc agaacatgtt
     1321 ttcgagccgg ttatgccgaa cgaccgctat acgaaaaggg cctctaaaca gaagtccact
     1381 ttgatccttg acaactgaaa cccaaaatca aacaagcagg ccttcgtcta aaaacgagcc
     1441 gaatgaagac cttgggggtt gcacccccaa acccccgtgc ccccgacaca ctaccgaaaa
     1501 ggtgggggtg ggtggcgaaa aaatcaaata aaacattcta ttcaagaaag gaagaaacaa
     1561 ccatgacaac taaaaacgag caactgaaag aacaggcaag aaaagagttg caagaaagag
     1621 gtttgattat tgaggggagc tttgaaggag attttgaaac ctatattggt tgttatgcaa
     1681 gaccgataaa caaacctaca gccttagacc caatgaacga acaagaagta aaagaacaag
     1741 aaaaacatgc agtgaatggt tttccgcaag atttcactga atggtatgag tgggaaatta
     1801 acaacgggaa gcttgaaaac ttcttataaa agtcagatga cgtgcaaaga gaatgattct
     1861 ctttgtacgg taaaaatata aagaaagaag gaaaaagaaa atggcaaaaa acacacctta
     1921 tcaagtagga gacatttttt atagtagttg gggctacgaa caaacgaacg tgactttttg
     1981 gcaaattgtg aagttaacag aaaaaacagc atggtttcag cctattcaaa agaaaacggt
     2041 cgagcattat tcgtccatga gtggaaaagt tgttccaatg aaagacaagt ttatagagta
     2101 tcctttagca aaaactaaag acagtattgt aaaaaaacga attgatccag aaacctatcc
     2161 tgatatgtta tgtggttatc gtagttggga cacgtttcat agatataaaa atcaagctgt
     2221 ttatgaaagt agttattatt aaggggatag tttccccttt caaaaataat aaatagaaga
     2281 atggagagaa aaaatcatga tgaattcagt gaatttagta ggacggttaa caaaagaagt
     2341 ggatttaaaa tacacaccaa aaggcaatgc gacaggcaca tttattttag ctgttaatcg
     2401 aaattatacg aatgcaaacg gcgagcgtga agcagattat atccgctgtg tcatttggcg
     2461 gaaagctgcg gaaactttag caaaatatac tggaaaagga agtttgattg gcattaatgg
     2521 acgtcttcaa acgaggagct atcagaatca gcaaggacag accatgtatg ttacggaagt
     2581 tctagtgaca gatttctatt tgttagagag taaagaaatc aatgagcaac ggtcaaaatc
     2641 aatggcgaat aatcaaaata attcttcaat tccaggaaat gagattcaaa tttcagatag
     2701 ttatttgccc ttttaagtga aaaataattt tgttctatac ctgatttctt caggtataga
     2761 acaaaaaata aatagagatg aggcgatttt ttatgaaaat cattgacagt tacgatcaac
     2821 gaaccatgca atatttgggc tcgtttgatt atacagatga aaatattatt aattatgttg
     2881 caagtttacc tccttatcaa agcgttcgct tggtagattt tgcaagtgat gaattggtat
     2941 taacaacgat gggcaacttt ttagattatg tcggagacaa aaaatggttg gaaaaaattt
     3001 tgtctaaatt gattgcgaaa caaacaggag aagaacccat aaatgcgatt caattgttca
     3061 atcgatatga acaagaaaac aatgattaaa gaggagagaa aaaatgaaaa gattaaaacg
     3121 aagtattttt attgtgctag cattgattga ttttgttggg acaggttgtg aagcaattga
     3181 cgttcaaaaa gctcccttat cggaaataaa acaagcaata acaaaacaag atgaagaaca
     3241 accgctaaat acacaagtag aaatagtgaa tcaaaataaa ccgagttttt ctgaagaaga
     3301 tttttcgtta gaaaatggaa ccaggcaatt tttttcagat ttggatcgtt taaatcgggt
     3361 aggtgtagca agtgcacttt tgcataaaag caggatgcca acagaagaac gagaccgatt
     3421 atacattaag ccaactggct ggaaacaaaa gaaaatgaag aatggcgatt atctttataa
     3481 tcgttgtcat ttgattggct atcaacgtgc gacaagtttc cccataagta tgtaatacga
     3541 aacgggagga ttaatcttaa gattaatcac aactaaccta tttgacagtt ggaatgaaag
     3601 ctatcatgtc tgctgaatca actggtctga tactcctaca tgcaataaag tctactagga
     3661 agttattgta tgaagctagg taaaggcggc agaacaattt ctaagtgtgg aatcatatga
     3721 agttggatat gccgggacgc tataaattac tcagggtgag actgttttta attaaactga
     3781 cgaacttgcg aatgtacggg tctataaccg acttatattc gaaagatata agattgcgaa
     3841 tgcaaagaac attatcgaaa agtaagaatg ttggtcatga aattcgagat ggaagactag
     3901 cggtgtatct gtcaagagtt ggtaaaacat gtttgattga ttcagatgag agtgggaatg
     3961 cgacaaaacg ttttacagaa gaaatagagc aactagcaga actgaatata cgatattttt
     4021 aaatacttta gcggagaata aaaaaatcct ctacagaaaa ctacggaggg caacatgtgc
     4081 tattgtttca acaatacatt tttaattttt accataacaa aaaagtactt gaaaaaaaac
     4141 agaaatatgt taaattaaaa atgaaaaaat ggaagcggtt acttattctt ttgaatctaa
     4201 gataaaaatt caaaagaata acataaccca aatatgatta tacttgagat tctaaatatt
     4261 tatcgaaaag gaatttgttt taaggaaaaa tgattctatg gacggttatt gatttatatt
     4321 taccaaatgg ctaataaaat agtatttctt atattgtata gtttttgttt ttatgtgaaa
     4381 acatgagcaa tagaattgta ctagtaggat aaggatggat ggaaaatgaa aaaaactatt
     4441 tttgttttag cgatttttat atgttttggg ctacttttgg taggctactt gttgtttaat
     4501 tctaatgaaa tgagaactat ttctacgaat gacttgttaa aaaaaagtga attaaaggat
     4561 aaaaaaaaag aatctcttgc ctttataatg tttactcaag aagagtgtga gtattgtata
     4621 gcaataaatc caatcataga tagtgtaaat aaggaaactt tgaataagat aaagaaatat
     4681 gatatatctg tagattttca atctaaacaa aagtgtaaaa ttaagtttgt tccgactatt
     4741 gttgcatata gtaagaataa agaagtgggg agaattgaca atgaattaga taatttgtat
     4801 cgaaaaaagc aagatggttc aattgtaata gacaagaaaa aaacaaagac attgcttata
     4861 gattggatga aaaaaatgga taaacaagga gaatatttgt atggagaaga ttaaacagac
     4921 atttccgtta attaataaat attggaataa gaaaaaaatt tttatatttt ttattacgtt
     4981 tctgctgtca tctttatttt ctctaatttt cccgtatcta tttagtaatg ttattgattt
     5041 atatttagag aatgagttat cacagaatat tgtgcgaata gtaacgattc ttatatttca
     5101 gttattttta gtcttaatta aagaagtgta tggatatata aaaactattt atgcatttga
     5161 agttgattta tcaatgaaaa ttgattttct taaggtttta caaaaaaaaa atcttttttt
     5221 ttcgcaaacg aagcattcag gagaaattca atttaggatg ttttcggata tttctactat
     5281 cgtaaatact atctttattt tttctgttga attacctgtg ataggcgttt tagttcttgt
     5341 agcggtattt attataagta aagtgaatct tatttttctg attttttcac taataatgat
     5401 gggagctatg gtgttacagt ataatttatt tcaattaccg cttaagaaaa caatcgagaa
     5461 tacaaaaagt gttaatcaaa gtattacagg agaattgaat gcacattttg atcaattaga
     5521 aattataagc gcatataatt ttgagaatat ctctatttta aaattcgatt ctagatttaa
     5581 agaattattt aatgttttca aaaaacaaaa taaattacta acactgggaa gctgtttggc
     5641 acaggttatt t
//
LOCUS       DARBFD010000131         5611 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000131 DARBFD010000000
VERSION     DARBFD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.136"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(59..2008)
                     /locus_tag="SCK08_002589"
     CDS             complement(59..2008)
                     /locus_tag="SCK08_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG1202422.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="SCK08_002590"
     CDS             complement(2050..3108)
                     /locus_tag="SCK08_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEG1202423.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="SCK08_002591"
     CDS             complement(3227..3682)
                     /locus_tag="SCK08_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202424.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="SCK08_002592"
     CDS             complement(4266..5069)
                     /locus_tag="SCK08_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1202425.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2073 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat a
//
LOCUS       DARBFD010000132         5595 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000132 DARBFD010000000
VERSION     DARBFD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.137"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..863
                     /locus_tag="SCK08_002593"
     CDS             6..863
                     /locus_tag="SCK08_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1202426.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            885..1394
                     /locus_tag="SCK08_002594"
     CDS             885..1394
                     /locus_tag="SCK08_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1202427.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1431..1832
                     /locus_tag="SCK08_002595"
     CDS             1431..1832
                     /locus_tag="SCK08_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202428.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1944..2297
                     /locus_tag="SCK08_002596"
     CDS             1944..2297
                     /locus_tag="SCK08_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202429.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2331..2774
                     /locus_tag="SCK08_002597"
     CDS             2331..2774
                     /locus_tag="SCK08_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1202430.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2791..3876
                     /gene="uxuA"
                     /locus_tag="SCK08_002598"
     CDS             2791..3876
                     /gene="uxuA"
                     /locus_tag="SCK08_002598"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1202431.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3869..4582
                     /locus_tag="SCK08_002599"
     CDS             3869..4582
                     /locus_tag="SCK08_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1202432.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(4789..4938)
                     /locus_tag="SCK08_002600"
     CDS             complement(4789..4938)
                     /locus_tag="SCK08_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202433.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            5334..5534
                     /locus_tag="SCK08_002601"
     CDS             5334..5534
                     /locus_tag="SCK08_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1202434.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1941 a          856 c         1216 g         1582 t
ORIGIN      
        1 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
       61 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
      121 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
      181 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
      241 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
      301 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
      361 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
      421 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
      481 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
      541 tcactatggg ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg
      601 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
      661 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
      721 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
      781 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
      841 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
      901 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
      961 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     1021 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     1081 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     1141 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     1201 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     1261 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     1321 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     1381 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     1441 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     1501 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     1561 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     1621 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     1681 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     1741 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     1801 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     1861 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     1921 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     1981 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     2041 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     2101 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     2161 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     2221 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     2281 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     2341 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     2401 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     2461 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     2521 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     2581 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     2641 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     2701 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     2761 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     2821 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     2881 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     2941 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     3001 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     3061 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     3121 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     3181 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     3241 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     3301 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     3361 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     3421 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     3481 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     3541 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     3601 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     3661 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     3721 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     3781 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     3841 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     3901 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     3961 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     4021 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     4081 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     4141 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     4201 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     4261 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     4321 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     4381 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     4441 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     4501 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     4561 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     4621 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     4681 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
     4741 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt
     4801 ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc
     4861 tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc
     4921 tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa
     4981 cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta
     5041 agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa
     5101 tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta
     5161 tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct
     5221 attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa
     5281 tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata
     5341 acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg
     5401 gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag
     5461 aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca
     5521 ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt
     5581 ttgtacgaaa aaatt
//
LOCUS       DARBFD010000133         5531 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000133 DARBFD010000000
VERSION     DARBFD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.138"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..985)
                     /locus_tag="SCK08_002602"
     CDS             complement(<1..985)
                     /locus_tag="SCK08_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEG1202435.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="SCK08_002603"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="SCK08_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1202436.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="SCK08_002604"
     CDS             1810..2964
                     /locus_tag="SCK08_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1202437.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="SCK08_002605"
     CDS             3179..4147
                     /locus_tag="SCK08_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1202438.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="SCK08_002606"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="SCK08_002606"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1202439.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5531
                     /locus_tag="SCK08_002607"
     CDS             5177..>5531
                     /locus_tag="SCK08_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HEG1202440.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1669 a         1078 c         1251 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc g
//
LOCUS       DARBFD010000134         5433 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000134 DARBFD010000000
VERSION     DARBFD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.139"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..145
                     /locus_tag="SCK08_002608"
     CDS             <1..145
                     /locus_tag="SCK08_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202441.1"
                     /translation="GSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNPVAGTYYVV
                     QNI"
     gene            270..2621
                     /locus_tag="SCK08_002609"
     CDS             270..2621
                     /locus_tag="SCK08_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEG1202442.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2701..3096
                     /locus_tag="SCK08_002610"
     CDS             2701..3096
                     /locus_tag="SCK08_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202443.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3421..4038
                     /locus_tag="SCK08_002611"
     CDS             3421..4038
                     /locus_tag="SCK08_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1202444.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            4079..4378
                     /locus_tag="SCK08_002612"
     CDS             4079..4378
                     /locus_tag="SCK08_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEG1202445.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            4412..4579
                     /locus_tag="SCK08_002613"
     CDS             4412..4579
                     /locus_tag="SCK08_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1202446.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4624..5229)
                     /locus_tag="SCK08_002614"
     CDS             complement(4624..5229)
                     /locus_tag="SCK08_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1202447.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         1859 a          758 c         1058 g         1758 t
ORIGIN      
        1 tggttctatt caaggaatgg cattgtatgc tcgtgaagga ttcaaatact ttgcaccaaa
       61 cgggcctggt tggacaatga atataggtat ttattacact tataatcctg ttgcaggtac
      121 gtattatgtt gttcaaaata tttaataaaa cagcacaaag agatttcttt gtgctgtttt
      181 attattttta gttgttgaca aacactaaaa tataaaatat aatcaacaca taaacgaacg
      241 tatgttcggc aagaaagcag gggattttta tggatgagat ggttttaagt actcagaaat
      301 ggctaaacaa gaaatacagt aatgttacag ggtttgataa ggttccagaa aatgggcgaa
      361 cagggtggcc tacaatctat ggacttattg aaggactaca agtagaactt ggtattacta
      421 atctagtagc taattttgga cctaccactg aaaaaatgta cgataatcag gttactccta
      481 aatggggcaa aaatctacct aaaaatattg tatttcttat tcaaggtgcc ttttggtgta
      541 aggggattaa tccaggtggc ttcgatgggg tatatactcc atatttagat actgctgtaa
      601 aagagttgca gactgatgca gggtttaatg gaagcgctgt aactggagtt ttggatgcga
      661 aatgggccaa agcgttattt gatatgagtg cttttgttct tgttcccggc ggagatagta
      721 aaattagaaa aatgcagcaa tggttaaatg taaattattt agaatacacg ggaataatgc
      781 cttgtgatgg tatttatcaa agaaattcaa atcaagcatt aatttttgca ttacaagctg
      841 aactaggata ttctcccagt gaagcaacag ggtcttatgg taatgggaca actagtaaaa
      901 cttatcctgt gtctgaaggt aactcgggaa actatgtaag aataattcaa tatggattat
      961 atgttaatgg ttattttgaa gacggtacat tcgatggtat tttcactaaa tatatgggtt
     1021 tagaagtact ggcattccga aaatttatgg tattgcctga atatactgaa atagcagatg
     1081 ttgttgtaat aaaaggacta ttgtctagcg ctggagatat tagaagatct gcagatggcg
     1141 cagatacttc tacgcaatta actagatctc aaatccaaac attggttgat aatgatataa
     1201 aaatagttgg tagatatctt actggtactg ttggaattgg aaaagatgaa cgaaataagt
     1261 atctaactac agaggaattg aataatattt tttctaaaaa tttatctgtg tttcctatct
     1321 atcaagatgg aggtgcagca ttagcttatt ttacttatga tcgtggctta tcagatggta
     1381 agaaggctat agatgcagca aaaaatctaa atataccatt aacaacagtt atttattttg
     1441 ctgttgatct agacatgtta ggggaagaaa ttcttgcata tgctggagaa tattttaagg
     1501 gggtatctgc tgtaatgtca tatagcgggt atcaaacggg tgtttacgga acaagaaatg
     1561 tttgttctca aataatcaat aacgggtatg cttcgtttgc atttgtttca gatatgtcga
     1621 ctggttatag tgggaattta ggttttccta tgcctagaca atggtctttt gatcaatttg
     1681 ttgaatttac cataggaagt ggcaacggag ctgttggtat cgatttaatc gctacatcgg
     1741 gtagagatag cggatttaat gagttatcaa atgatactaa taatgataat tacattgcca
     1801 aatacaatca gaaggttata aatcagatgg tacgtgcgta tcaatatcta tcacaagctg
     1861 gtcttgataa tccttggaac cctcatttga cattttatag atatgtaaat tattctggtt
     1921 tgtcatggga catcatttct tctcctgtaa cagagcatga tagaaaaatt tatgatgaat
     1981 ataggaataa attaactaat agcgagggcc tgtataatta ttttatagat cctaatactg
     2041 gttcaataat aggtttgcct cacttaattg tgacgttaca atcacagatg tttctagccg
     2101 acacaatcaa tgatagtgtt tctgactttg caggttggtt aggagattta atgacttgct
     2161 ggggtgaagt taagaaatta ggaatacaaa tggagcaagg tgtatttgca ttagtaggta
     2221 cggctgcagg tggaacattt agtctagaag atttatacca agatgttgac gcggtaaatg
     2281 tttatacctt agcaaaaaga aattctgatt caacagcttt atcggcttta aaaacgtact
     2341 attgtgataa gggtgctatt gatagattta atcaatttgt taagaacaga tttggttctt
     2401 cagataaaat cgcttcagta acaaatagtt tattagaaaa tgacgaaaca gttgatatct
     2461 taggtgtagg gataatttca tttttcattg ataaaatagt taataattca cttgggacag
     2521 attttactta tcttgatttt attggagatg aggggaaagt tgcaaatgga ttttctgaaa
     2581 aaattattca atttcaagaa aatagaagta aacttaatta agaatcgttt actatttaaa
     2641 cagtgtcctt ttccttttta aatatatacg ataaaataag tatatgaatt gtaaaaggac
     2701 atgatgatta tggaagaaat agaatggaat aatctgactc aaaataataa aaataattct
     2761 ggatgtggaa ttatctttat gtttgttata gttttgggaa tagtagtact tttaataact
     2821 gtcaagatag tagattactt tgcgaatact cctgaagaac aaatacagag aaaacttcct
     2881 gagtttgaaa gagtaccacc ttatgatcca agagataatt catttcaagt aaaaaaatta
     2941 aaagagtctc caaatgaaat gtggtttgaa gttacagttg aatcaagcga cttcaaagaa
     3001 aaaaagaaag aatacaatta tattgtaaag gcaaaagtat caaaaagatt tatttttaac
     3061 cctaaattag aaatatatag tgttgtaata gaataatagt gttaaaaaaa ttgtaaaagt
     3121 taaagaagtc aaattctgat gtgaaaaata aaatatacca tggaagacat aaaagaaaaa
     3181 ctggataact agtttattta atggcagacc gttaaggcct gccattttta tgtattttag
     3241 cggaataaat ataatcatgc gggagtgtaa acttactgtt tcaaaaagta tactttttga
     3301 catgccttga ttttctttgt ttatgtatgg cataataacc acataataca atttgaaacg
     3361 tttctgattt aatctgattg ttttgaaaca attttagtga aaaaagggta gataaaaacg
     3421 atgagaaaat ttttttatat ccgggtcagt agttatgatc aatcgatcga acgacaactg
     3481 atttccgcga aggaattagg aatccctgaa gaatatatat ttgtagaaaa agccagcggc
     3541 aaagatttta aacgccctga atatcaatta atgaaacgga tgtttcgaaa aggagattta
     3601 ctctatctac aatcattaga tagactaggc agaaataaac aaatgatttt agaagaatgg
     3661 cgagaattaa ctaaggaaaa gcagattgat atttctgtgt tagacatgcc tctgttagat
     3721 actgctagtt acaagcaact agatgggtta gagacgctta tctctgactt aattctgcaa
     3781 ttactcagct atatggccga agacgagcgg aaacgaatta atagccggca aagagaaggc
     3841 gtagaagctg cgaaacaaaa gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct
     3901 gaatttcctg cgatctattc tgactggaaa gcaggaaaga taactgcagt ctatgctatg
     3961 gatcaattgg gcatgaaacg aaataccttt tatcgacggg tgaaagaata tgaacatacc
     4021 ttgcaccact tttcttgata gatataacag ctattagacg ggaagggaga gtttgaaaat
     4081 ggacttaacg aatcaaaggg tcgtttttac tggaacgctg cagcaaatga cacgaaccca
     4141 agccatcggt ttggttcact ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac
     4201 aaatttcctg gtttgtggtc agtattctaa gtcgttgttt gatgattctc tctatacaaa
     4261 aaaggaacaa accgcgcggg acttgattgc gttaggtcat gagattacca ttttatcaga
     4321 agtcgaattt tatgcgttaa ttagtcaaca gttgaaagaa tggcaacggt atttataatc
     4381 gttagtaaaa tttacaagga ggaaaatttt tatgaagcga aaatcttatg atcgttgggg
     4441 cattgaaata actagtttta atcgaaatat tgcttttcac gtggagtgtg actgtgggga
     4501 attagcaaaa agtgtttatc aaaaaaatta cttttactgt cctaaatgcc atcgccgata
     4561 cggggtacat ggaacataga aacgaaatag tgcggtagaa aagcgaatga atgtagtata
     4621 tttctacaaa tcattcgctt tctttatttt ataaatagtt ggtcgagaga tcccgttttc
     4681 ttttgcaatc tctgcaactg gaatgccttg ctttaacatg tccaccacgg ttttaaaaac
     4741 taagcgtttt tgcggatctg ttgagttaat agaatactcg attggtcgtc ccttatatcg
     4801 gccggctttt tgagccaata atactccttg ccgctgacgt tcacgaattt tctcacgctc
     4861 gttttgtgca atataactta gcagtgataa aaacatatcg aacatagcgg tatccaggag
     4921 ttcatttcca gtattaaaat ctaaaaactg tgcatctaat atttttaacg ctactttttt
     4981 ctgtttcata aaagcaactg tattttttat atcttcataa tctcttccta aacgatctag
     5041 tgatgtaacg atgacgcaat caccttcacg gatataattt agtaattttt gaaactcagg
     5101 acgctcagta tctttaccac tggctttttc ttggaataat ttatcaatgt taagtccttc
     5161 catcattgcc aattgtcgat ccaaattttg atcttgtgaa gatactcgaa tatagccaat
     5221 ttttcccata aaaaactcct ttttaaaagt gatataattt acatgtaaaa tttacttgta
     5281 gttttactgc tatcatagca tttttaaaaa taaattttat gtagacttaa aatttacttc
     5341 atggagggtt taaatggatg aaatcaaaag taacaaaaaa gcatttggca tttatttagg
     5401 agaacctagt acagaacaat taaaccaatt ttt
//
LOCUS       DARBFD010000135         5342 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000135 DARBFD010000000
VERSION     DARBFD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.140"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(61..279)
                     /locus_tag="SCK08_002615"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="SCK08_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="SCK08_002616"
     CDS             complement(545..739)
                     /locus_tag="SCK08_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202448.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="SCK08_002617"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="SCK08_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2091..3131
                     /locus_tag="SCK08_002618"
     CDS             2091..3131
                     /locus_tag="SCK08_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1202449.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3955..4287
                     /locus_tag="SCK08_002619"
     CDS             3955..4287
                     /locus_tag="SCK08_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEG1202450.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4906..5247
                     /locus_tag="SCK08_002620"
     CDS             4906..5247
                     /locus_tag="SCK08_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEG1202451.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1771 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtggtat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cacaatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     4141 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     4201 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     4261 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     4321 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     4381 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     4441 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     4501 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     4561 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     4621 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     4681 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     4741 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     4801 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat
     4861 ataaaacata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     4921 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     4981 ggatttgtga cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattagac
     5041 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     5101 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     5161 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     5221 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     5281 tagaaggagt cacgatgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
     5341 tt
//
LOCUS       DARBFD010000136         5220 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000136 DARBFD010000000
VERSION     DARBFD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.141"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="SCK08_002621"
     CDS             complement(<1..1256)
                     /locus_tag="SCK08_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1202452.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="SCK08_002622"
     CDS             complement(1270..2487)
                     /locus_tag="SCK08_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1202453.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="SCK08_002623"
     CDS             complement(2477..4072)
                     /locus_tag="SCK08_002623"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1202454.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="SCK08_002624"
     CDS             4197..4982
                     /locus_tag="SCK08_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HEG1202455.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DARBFD010000137         5040 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000137 DARBFD010000000
VERSION     DARBFD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.142"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            414..620
                     /locus_tag="SCK08_002625"
     CDS             414..620
                     /locus_tag="SCK08_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEG1202456.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(822..1823)
                     /locus_tag="SCK08_002626"
     CDS             complement(822..1823)
                     /locus_tag="SCK08_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1202457.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1828..3741)
                     /locus_tag="SCK08_002627"
     CDS             complement(1828..3741)
                     /locus_tag="SCK08_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1202458.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3909..4415)
                     /locus_tag="SCK08_002628"
     CDS             complement(3909..4415)
                     /locus_tag="SCK08_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1202459.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4575..5015
                     /locus_tag="SCK08_002629"
     CDS             4575..5015
                     /locus_tag="SCK08_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1202460.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
BASE COUNT         1391 a         1008 c          769 g         1872 t
ORIGIN      
        1 ttatttttgt ttcttatttt ctaaaaaatt aaaaaaaatt ttggtgaatt aagtatttcc
       61 ttgataacac tggcttttgg cctcatttta ggttatcatt tgtaatttta tttcacagtt
      121 gatatgattg ttatgtaaaa taaaaaagtt taggatacat cgaatttttg gggaaattct
      181 tttgggggta tcctaaatct tttctattat attcccatat tttctattat tggtctagac
      241 gtatgatggt aaactttgta cctatctgat tgctgaatca ttaattgctt atcattagcc
      301 atctttgttt caagttcgct agatgatcat tttattcaca tcctaacaaa cttgtcacat
      361 ttatagattc ttaatctatt tatcatacaa ttataaaaaa ggaggtgctg cttatgaaag
      421 ctatccatat ttctttgctt tttttcgcta gtgcattgat tttatttgct attcatcttt
      481 gttcccagcc ttatatcaat aacaacggaa tattagtgga accagcattc ttttgcttac
      541 cactcggctt tttgtccctt cttgcctccg caggcttcgg atttgttgct ttttttaaac
      601 gatcaaaaca acaaatctga cttataaaat ttacgtaaaa gagatcgtga caataatcgt
      661 gtgatattcc caaatttcga ataaaaggtg ccccttgcca tctctaactc ctcagtcaca
      721 agttacaata ttcataaacc atgaaaagct tgtttattga aattgctcct tgtgacttgg
      781 agctaagtga cagattacaa cctcttttgc atcataagac tttactcctt gaaaaacatt
      841 actaactttt gatatgtttc ttgcgcttgt ttattgctga aatcaaactg atattcatgg
      901 ttcaaaacag catttttctc cgcataatct acttttgtga ctttcacccc cgctttctca
      961 aatacagaag ttacttcttc catttgtttc gtaaacgtat ttgtatttcc atccgttaag
     1021 aatgtgtttt gcgtccaacg atccgcatgt ctgaccaagg ctaaactttt gaccttctct
     1081 gattgttcaa agtccttatt atcaaagtaa gcccatccac atttttcata aagaaagcta
     1141 gagattttat cacttacttg agtaagtgca gaaggatcca acagaccaga caaagaaata
     1201 aacttcgtaa tctgcttgtt ctccaaagtc ggatcaagat gatcagcttt tttaatttct
     1261 tcattttgaa gtgctaacaa atattctcca ctaatttgtg cacctgccga atttccacca
     1321 atcgaaatat tcgaccaatc tgcttgatct tttatctctt ttttagctgc ttgaatggct
     1381 tcgttcaatt ggataactgg tgtaggatgt tttgcttccg gtgatcgttc ataatctata
     1441 tttacgatga tttgctgcgt gtctgaacat agatattcca aataatcacg acaatcattt
     1501 ttatcccctc ctacatatgc gcctccatgc atccagaaaa gaatcggtaa ttttttcttt
     1561 tccactttcg gtgtgtagat atccatatta ttatttttgt actttgaagg ataggatatt
     1621 tcttttttta tatcgatttg ttcgattttt tgctggaaat catctggtcg ggcatatact
     1681 tgttcttttt cacttgtgtc aaattgtttt ctaacaagca agctaatcgg ctttggtgtg
     1741 atttgattaa tcaaaaatac agtgactaaa ataactacga tgataactgg tactgcaatc
     1801 actattcgct tgaaccattt cattcattta ccctctcttt cttcgccaaa tccatgtgat
     1861 aatcgtgcta acagataaaa atccacctga ataaatgaga agcgaatgtt tcttttctcc
     1921 agttttcggc aacattcttt tttttgcatc tgtctttttt tgtactttca ctgtcatctg
     1981 aatcggttgc gtatcatcac ttgattgatc ctctcgaggt tcattctttc ccttattgtc
     2041 tgcattctct gtcggatctt gcttttttgg tggagctact gggatttttt tgtaatagaa
     2101 aataatagtt ggtatcgtct cattaaaaat cccttcagtc ggtccttcaa catttgctaa
     2161 ttcgtatcct tcgaatgtct tttgctctgt catataattt tctccataag ctccgtaaag
     2221 ctggatttcg tcctggtctt tgagtagact tttattgttt tcatccacat actttacgtt
     2281 tatctgatca tggaacacta ctttcaaaaa gtccgttaca tgtttcgtgt aggcagcatg
     2341 gtcaacttcc tgtgcctttc catgccctgc tccttctact aataaaagat ctttataccc
     2401 tcctgataac tcatataatt tttgcgcgtt agacacaggt actacatcat ctgctgttcc
     2461 atggataatc aatttaggca atgaatctga cattccaatc gtaggtagag cagcttttgt
     2521 atccaaatgc atctcctgat caaaatagta ttgtttcaaa aggcccataa ataaatcttc
     2581 atgctcttga tccccaatga tgtctaactg attttttatg gcatccggta gtgcgggggc
     2641 tactgtctgg ctgtacgtaa aacgtagttg ttcatcaatt gatgcaaatc cgcaattttc
     2701 aataattcct ttgacattga caggaatatc tttcgctaat acagacatca tcgtagctgc
     2761 tcccatcgaa ccaccataaa gtaaaatctt ttgagatggc cgctctcgaa cttcttgatt
     2821 tatccagtaa aggacgtcat ctgactcata ttgtccatag gtcacataat cgcctccgct
     2881 attaccagtc gctctggaat caacaaataa taaattatac ccggccttat aaaaatcatt
     2941 gtattcttct gtaacgatcc cgttattcca attccctcga aatccgccat ggatcatgac
     3001 tgttttatct gaaccttgat cgacataata tgcatataca tccatcattt tgccatcttg
     3061 tttgtttagt acagtttgat gaagttgttt tgtatttgga tagaagcctg tgttttctgt
     3121 aggcgttgtc ccatcatctg tacctccaag catcgacatc aacacactgt tttttgcaaa
     3181 agcgatatcg tacaatagat aagtagcaat cagatgtttg ataatctctt tatctacacc
     3241 ataatcatta acagcaagct cagtgactaa taactctatt aattttgact tgatgtcttc
     3301 tgttggtcca ttaagtattt ctgtcatctg tttaccgaag taattcaaat attctttacc
     3361 attgaaattt tcacctttac cgatatcatc attttctgtc ccattctctt tgtcacttac
     3421 tttagcagaa gaggattcgt cgtttgacat ttttgtagct gttgcttctt ctgtttgttg
     3481 gttatctgtg gtagcggtcg tttcttgatc tgtgctagta acagatgtct caaccgttgt
     3541 ttcagtattt gtttctgttt gtgaagtact cgatttcgtt tcatttccag catcagtagt
     3601 ttctgtggtt tctacctccc gagattccga ttcggaagaa atgctctctc catatactgt
     3661 ccattgcggg acgcttgtac ttacagcaaa taagatgacc aacgctccct ttaatacttg
     3721 ttttgttctc aagctattca ctccttttta tgtttctagg agaattatat gtaaacgcaa
     3781 acatttttta tacaaaaaat aaatggttta tcccataaaa atagaacagg attattttct
     3841 cattttacgt aataaagcag aatggataaa ccagtatcca ttctgctttt tttaaacgaa
     3901 gaaacaactt attgctccaa aaatgtcaga aatcgtttat cccaaatttt ctcgtaaaaa
     3961 gcgattgttt cctttgcttt cataaaagga ttatcataag ttgtcgttgc tcctctacac
     4021 atcataagct ggtatcctaa tccatgagca aacttcacag tcgtatctat acaatactct
     4081 gcttgtgccc cacataattc gatcgaatga cagtccaatt cagttaataa aacactcaaa
     4141 ttagtcttat aaaatgaatt cgcatgggtt tttctcacaa aataactgtg ctcaggaata
     4201 gataattccg acaatagctc ccatggtttt gtttgtggca ctaattgagc atcttcgtgt
     4261 tgaacgaaaa taactggttt cccaatcgtt tcgtatgttt tgattctttt attcactaat
     4321 gctgtcactg tttctaattc gtgtatcggc gaagaatcaa aacatacccc tttttgtaaa
     4381 tcgatcacta ctattgcgtc ggctgtgttt gacatacttg tgccttcttt cttttttctt
     4441 gatttaatag tatttcttat ctagctagtt ccattatacg tgacaacatt ttcttatttt
     4501 acacctctaa atggcacttt tcgactattt cttcatgtta ttatttcata aatacttagc
     4561 tattggagga atgtatgatg attaaattgt atacaggttc tggctgctcg tcttcaagac
     4621 gcgcaaaacg ttggcttatc gtccatgata taccgtttat cgaacagaac ttaattcgtc
     4681 aaccactaac caaaaatgaa tttatccact tactaaaatt gacaaacaac ggcgtgaccg
     4741 aaatgctctc tactcattct attagttata aaaatcttgt tcacagaata gatgaactca
     4801 gtttgagtga actatatatt gagattcgta catccccttc tttattgaaa aaaccgatta
     4861 tctacgacca atctgcaaaa cttttgactg gatttagcga agataaaatc aggatgttcc
     4921 tttctaaaac taaaagaaag caagagctca acgaactctt gctttctggt catcttgtcg
     4981 attttaaaga aaataatctt tttgcaatgt cttaattttg gcataaacaa tctgcttttc
//
LOCUS       DARBFD010000138         5029 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000138 DARBFD010000000
VERSION     DARBFD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.143"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="SCK08_002630"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="SCK08_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEG1202461.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="SCK08_002631"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="SCK08_002631"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEG1202462.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="SCK08_002632"
     CDS             complement(2169..2906)
                     /locus_tag="SCK08_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1202463.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="SCK08_002633"
     CDS             complement(2997..3524)
                     /locus_tag="SCK08_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202464.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="SCK08_002634"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="SCK08_002634"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEG1202465.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="SCK08_002635"
     CDS             complement(4401..4886)
                     /locus_tag="SCK08_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEG1202466.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1336 a         1123 c          862 g         1708 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgttt
//
LOCUS       DARBFD010000139         5004 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000139 DARBFD010000000
VERSION     DARBFD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.144"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..182)
                     /locus_tag="SCK08_002636"
     CDS             complement(<1..182)
                     /locus_tag="SCK08_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1202467.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="SCK08_002637"
     CDS             517..951
                     /locus_tag="SCK08_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1202468.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="SCK08_002638"
     CDS             974..1294
                     /locus_tag="SCK08_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202469.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="SCK08_002639"
     CDS             1435..1626
                     /locus_tag="SCK08_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202470.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="SCK08_002640"
     CDS             complement(1697..1954)
                     /locus_tag="SCK08_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEG1202471.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="SCK08_002641"
     CDS             2112..2375
                     /locus_tag="SCK08_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202472.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="SCK08_002642"
     CDS             2399..2587
                     /locus_tag="SCK08_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202473.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="SCK08_002643"
     CDS             complement(2670..2855)
                     /locus_tag="SCK08_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202474.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="SCK08_002644"
     CDS             3070..3441
                     /locus_tag="SCK08_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1202475.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="SCK08_002645"
     tRNA            complement(3591..3663)
                     /locus_tag="SCK08_002645"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="SCK08_002646"
     CDS             complement(3709..4137)
                     /locus_tag="SCK08_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202476.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="SCK08_002647"
     CDS             complement(4197..4955)
                     /locus_tag="SCK08_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEG1202477.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1743 a          784 c          978 g         1499 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt attt
//
LOCUS       DARBFD010000140         4897 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000140 DARBFD010000000
VERSION     DARBFD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.145"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(406..780)
                     /locus_tag="SCK08_002648"
     CDS             complement(406..780)
                     /locus_tag="SCK08_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1202478.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="SCK08_002649"
     CDS             complement(803..2203)
                     /locus_tag="SCK08_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEG1202479.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="SCK08_002650"
     CDS             complement(2207..2617)
                     /locus_tag="SCK08_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1202480.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="SCK08_002651"
     CDS             complement(2635..3126)
                     /locus_tag="SCK08_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1202481.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="SCK08_002652"
     CDS             complement(3136..3927)
                     /locus_tag="SCK08_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1202482.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="SCK08_002653"
     CDS             complement(3920..4693)
                     /locus_tag="SCK08_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1202483.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1556 a         1001 c          647 g         1693 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcct
//
LOCUS       DARBFD010000141         4788 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000141 DARBFD010000000
VERSION     DARBFD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.146"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            564..989
                     /locus_tag="SCK08_002654"
     CDS             564..989
                     /locus_tag="SCK08_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202484.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="SCK08_002655"
     CDS             1019..1183
                     /locus_tag="SCK08_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202485.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="SCK08_002656"
     CDS             2141..3118
                     /locus_tag="SCK08_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1202486.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="SCK08_002657"
     CDS             3128..3625
                     /locus_tag="SCK08_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1202487.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="SCK08_002658"
     CDS             4001..4282
                     /locus_tag="SCK08_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202488.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="SCK08_002659"
     CDS             4284..4700
                     /locus_tag="SCK08_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202489.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          816 c          785 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtatacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DARBFD010000142         4720 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000142 DARBFD010000000
VERSION     DARBFD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.147"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="SCK08_002660"
     CDS             complement(<1..1097)
                     /locus_tag="SCK08_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1202490.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="SCK08_002661"
     CDS             complement(1227..2603)
                     /locus_tag="SCK08_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1202491.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="SCK08_002662"
     CDS             complement(2603..3259)
                     /locus_tag="SCK08_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1202492.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="SCK08_002663"
     CDS             complement(3269..4546)
                     /locus_tag="SCK08_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1202493.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..>4720)
                     /locus_tag="SCK08_002664"
     CDS             complement(4565..>4720)
                     /locus_tag="SCK08_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEG1202494.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1477 a          858 c          566 g         1819 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DARBFD010000143         4711 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000143 DARBFD010000000
VERSION     DARBFD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.148"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            106..357
                     /locus_tag="SCK08_002665"
                     /pseudo
     CDS             106..357
                     /locus_tag="SCK08_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="SCK08_002666"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="SCK08_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG1202495.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="SCK08_002667"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="SCK08_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="SCK08_002668"
     CDS             1786..1869
                     /locus_tag="SCK08_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG1202496.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="SCK08_002669"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="SCK08_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG1202497.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="SCK08_002670"
     CDS             complement(2994..3857)
                     /locus_tag="SCK08_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEG1202498.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="SCK08_002671"
     CDS             complement(3859..4131)
                     /locus_tag="SCK08_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1202499.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="SCK08_002672"
     CDS             complement(4148..4357)
                     /locus_tag="SCK08_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEG1202500.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4711)
                     /locus_tag="SCK08_002673"
     CDS             complement(4455..>4711)
                     /locus_tag="SCK08_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEG1202501.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc a
//
LOCUS       DARBFD010000144         4646 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000144 DARBFD010000000
VERSION     DARBFD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.149"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(133..717)
                     /locus_tag="SCK08_002674"
     CDS             complement(133..717)
                     /locus_tag="SCK08_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1202502.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="SCK08_002675"
     CDS             complement(928..1131)
                     /locus_tag="SCK08_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202503.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="SCK08_002676"
     CDS             complement(1141..1563)
                     /locus_tag="SCK08_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202504.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="SCK08_002677"
     CDS             1801..2415
                     /locus_tag="SCK08_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1202505.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="SCK08_002678"
     CDS             complement(2580..3404)
                     /locus_tag="SCK08_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202506.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="SCK08_002679"
     CDS             complement(3428..3583)
                     /locus_tag="SCK08_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202507.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="SCK08_002680"
     CDS             complement(4042..4299)
                     /locus_tag="SCK08_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1202508.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="SCK08_002681"
     CDS             complement(4292..4561)
                     /locus_tag="SCK08_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1202509.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttat
//
LOCUS       DARBFD010000145         4633 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000145 DARBFD010000000
VERSION     DARBFD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.150"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            175..519
                     /locus_tag="SCK08_002682"
     CDS             175..519
                     /locus_tag="SCK08_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEG1202510.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="SCK08_002683"
     CDS             520..939
                     /locus_tag="SCK08_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEG1202511.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="SCK08_002684"
     CDS             complement(1031..1381)
                     /locus_tag="SCK08_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202512.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2567)
                     /locus_tag="SCK08_002685"
     CDS             complement(1347..2567)
                     /locus_tag="SCK08_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEG1202513.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="SCK08_002686"
     CDS             2933..3499
                     /locus_tag="SCK08_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1202514.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="SCK08_002687"
     tRNA            3644..3731
                     /locus_tag="SCK08_002687"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4633
                     /locus_tag="SCK08_002688"
     CDS             4065..>4633
                     /locus_tag="SCK08_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEG1202515.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1683 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tgg
//
LOCUS       DARBFD010000146         4610 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000146 DARBFD010000000
VERSION     DARBFD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.151"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(85..339)
                     /locus_tag="SCK08_002689"
     CDS             complement(85..339)
                     /locus_tag="SCK08_002689"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1202516.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(450..704)
                     /locus_tag="SCK08_002690"
     CDS             complement(450..704)
                     /locus_tag="SCK08_002690"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1202517.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(897..1172)
                     /locus_tag="SCK08_002691"
     CDS             complement(897..1172)
                     /locus_tag="SCK08_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEG1202518.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1144..2097)
                     /locus_tag="SCK08_002692"
     CDS             complement(1144..2097)
                     /locus_tag="SCK08_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1202519.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2709..4202
                     /locus_tag="SCK08_002693"
     CDS             2709..4202
                     /locus_tag="SCK08_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEG1202520.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4337..>4610
                     /locus_tag="SCK08_002694"
     CDS             4337..>4610
                     /locus_tag="SCK08_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEG1202521.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1542 t
ORIGIN      
        1 tgcttgaatt cttgtgaaaa agttcttcgt gttctcttag acataaaaat tcctcctggt
       61 atgttttctt ctagtctaca caccttaatt tttctgtcta gttaattgta gcctatccat
      121 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
      181 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      241 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      301 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      361 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      421 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      481 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      541 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      601 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      661 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      721 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      781 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      841 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      901 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      961 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     1021 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     1081 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1141 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1201 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1261 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1321 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1381 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1441 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1501 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1561 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1621 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1681 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1741 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1801 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1861 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1921 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1981 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     2041 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     2101 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2161 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2221 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2281 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2341 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2401 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2461 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2521 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2581 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2641 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2701 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2761 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2821 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2881 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2941 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     3001 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     3061 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3121 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3181 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3241 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3301 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3361 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3421 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3481 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3541 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3601 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3661 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3721 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3781 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3841 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3901 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3961 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     4021 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     4081 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4141 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4201 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4261 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4321 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4381 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4441 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4501 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4561 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa
//
LOCUS       DARBFD010000147         4473 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000147 DARBFD010000000
VERSION     DARBFD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.152"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            321..1052
                     /locus_tag="SCK08_002695"
     CDS             321..1052
                     /locus_tag="SCK08_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1202522.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1197..3224
                     /locus_tag="SCK08_002696"
     CDS             1197..3224
                     /locus_tag="SCK08_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1202523.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3332..4429
                     /locus_tag="SCK08_002697"
     CDS             3332..4429
                     /locus_tag="SCK08_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1202524.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaatataat atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc
       61 aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct
      121 gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga
      181 taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca
      241 taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa
      301 ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt
      361 tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat
      421 ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg
      481 atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa
      541 caggaggggc attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt
      601 ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac
      661 cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct
      721 ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat
      781 ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt
      841 ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa
      901 caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg
      961 tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag
     1021 tttggacatt agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa
     1081 tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat
     1141 ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga
     1201 aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca
     1261 tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca
     1321 aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg
     1381 atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca
     1441 aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta
     1501 tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg
     1561 ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata
     1621 agaagattag tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac
     1681 gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta
     1741 atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg
     1801 gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt
     1861 cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt
     1921 ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag
     1981 ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc
     2041 aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc
     2101 tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt
     2161 tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg
     2221 atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga
     2281 taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga
     2341 ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc
     2401 aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt
     2461 attttattgc tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc
     2521 ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat
     2581 cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata
     2641 aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag
     2701 agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga
     2761 atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca
     2821 aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta
     2881 gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt
     2941 ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc
     3001 ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg
     3061 ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta
     3121 cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg
     3181 aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata
     3241 taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga
     3301 gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc
     3361 aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc
     3421 agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga
     3481 caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc
     3541 ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac
     3601 caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct
     3661 gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag
     3721 tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc
     3781 tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca
     3841 aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact
     3901 gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag
     3961 aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat
     4021 tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg
     4081 attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct
     4141 aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa
     4201 attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa
     4261 agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa
     4321 aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt
     4381 atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa
     4441 caaatagtaa atttttatta ttgtaggatg gtt
//
LOCUS       DARBFD010000148         4434 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000148 DARBFD010000000
VERSION     DARBFD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.153"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(543..2048)
                     /locus_tag="SCK08_002698"
     CDS             complement(543..2048)
                     /locus_tag="SCK08_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1202525.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="SCK08_002699"
     CDS             complement(2045..2731)
                     /locus_tag="SCK08_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1202526.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="SCK08_002700"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="SCK08_002700"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEG1202527.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DARBFD010000149         4368 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000149 DARBFD010000000
VERSION     DARBFD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.154"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..1257)
                     /locus_tag="SCK08_002701"
     CDS             complement(37..1257)
                     /locus_tag="SCK08_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEG1202528.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="SCK08_002702"
     CDS             complement(1277..1888)
                     /locus_tag="SCK08_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1202529.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="SCK08_002703"
     CDS             complement(1931..2863)
                     /locus_tag="SCK08_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG1202530.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="SCK08_002704"
     CDS             complement(3284..4144)
                     /locus_tag="SCK08_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1202531.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
     gene            complement(4276..>4368)
                     /locus_tag="SCK08_002705"
     CDS             complement(4276..>4368)
                     /locus_tag="SCK08_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1202532.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIQA"
BASE COUNT         1242 a          881 c          716 g         1529 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag cttggattcc caataatacc ttgatttcag tacagaccga
     4321 aaacccgaag agagtgcctt cttttcgggt tttcttatat aatcctcg
//
LOCUS       DARBFD010000150         4345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000150 DARBFD010000000
VERSION     DARBFD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.155"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(387..1853)
                     /locus_tag="SCK08_002706"
     CDS             complement(387..1853)
                     /locus_tag="SCK08_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1202533.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SCK08_002707"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SCK08_002707"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1202534.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DARBFD010000151         4324 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000151 DARBFD010000000
VERSION     DARBFD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.156"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..281)
                     /locus_tag="SCK08_002708"
     CDS             complement(<1..281)
                     /locus_tag="SCK08_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1202535.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="SCK08_002709"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="SCK08_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="SCK08_002710"
     CDS             complement(2359..2721)
                     /locus_tag="SCK08_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202536.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3063)
                     /locus_tag="SCK08_002711"
     CDS             complement(2725..3063)
                     /locus_tag="SCK08_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202537.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3042..3461)
                     /locus_tag="SCK08_002712"
     CDS             complement(3042..3461)
                     /locus_tag="SCK08_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202538.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="SCK08_002713"
     CDS             complement(3454..3687)
                     /locus_tag="SCK08_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202539.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="SCK08_002714"
     CDS             complement(3684..>4324)
                     /locus_tag="SCK08_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1202540.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DARBFD010000152         4026 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000152 DARBFD010000000
VERSION     DARBFD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.158"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(242..391)
                     /locus_tag="SCK08_002715"
     CDS             complement(242..391)
                     /locus_tag="SCK08_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202541.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="SCK08_002716"
     CDS             853..1065
                     /locus_tag="SCK08_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202542.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="SCK08_002717"
     CDS             complement(1343..3142)
                     /locus_tag="SCK08_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1202543.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="SCK08_002718"
     CDS             complement(3266..3862)
                     /locus_tag="SCK08_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1202544.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          688 g         1178 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataac
//
LOCUS       DARBFD010000153         3986 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000153 DARBFD010000000
VERSION     DARBFD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.159"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(9..551)
                     /locus_tag="SCK08_002719"
     CDS             complement(9..551)
                     /locus_tag="SCK08_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEG1202545.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            686..1075
                     /locus_tag="SCK08_002720"
     CDS             686..1075
                     /locus_tag="SCK08_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202546.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1079..1843
                     /locus_tag="SCK08_002721"
     CDS             1079..1843
                     /locus_tag="SCK08_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1202547.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1972..2919
                     /locus_tag="SCK08_002722"
     CDS             1972..2919
                     /locus_tag="SCK08_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1202548.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3049..3789
                     /locus_tag="SCK08_002723"
     CDS             3049..3789
                     /locus_tag="SCK08_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202549.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1321 a          683 c          810 g         1172 t
ORIGIN      
        1 tttgtgagtt attttccttg tcgttttctc acttttttag tccagaaacc acctgaaata
       61 tattttggct cataagaaat gataaaagca cgctcttcga tttctttgat caggcgatac
      121 agttcacgtt cgctttttct aggagaaagt atttcgagaa ctagccgctc accttccagc
      181 cccattgcag aagtctgtgt cacaccataa ccgtgttctc tcagtatacc cggcaaactt
      241 ttatcttctg cgctatttgc tggcaaaatc gttgtgacca tgatgtatcc taatgccaat
      301 ttgtcttcaa tcaaaattcc gacactgata cctactgcgt aacctaacgc atagacaaat
      361 aaattcagtg gattatctaa acgatctaaa acaagggata agccgacgat ataaatcgtg
      421 atctcgacca tactgacaag tggagcaatc agtcgatagc ctttcattgt caacatgaaa
      481 cgtaacgtgt tcagagtgat ataagcaaaa ttaatgagga agatcatccc caacattttt
      541 ggatcaatca tttgtaaaac tcctttcatt tatataacaa caatatcaaa aaaaatcgta
      601 agtgaagaaa aattgaaact ttatacatac aaaaaaatga taaaatgatt ataatagaat
      661 tataaagata ggttaggtga ttaaaatgaa aacggtcaat cgtgtgtttt gtgccagcca
      721 cgtgctaagt ctccttctca gtatatactt aggtgtctat tcgattcaac agccaattga
      781 ggtagagaac attgtttttc agatgtcgct gacagatggt ttaattttaa gcgttttttt
      841 ctttttgata ctgtttattg gtaatatttt cggtagcgtt gccagttttt tgaatttttc
      901 tatttatcca ctcctttcgc tagcattagg agtggtggga ctttgttctc tgtgcttatt
      961 tcccgagcca ttcagtcctg catttatatt atttggtaca ctgaacctgt tccagacaac
     1021 tgttggagca tggttgctat ggcgatcagg aaatttaatg aaaataggtg agtaacaatt
     1081 gaatccaatt atattgaaac gttctgtcca tcatattcca gtgttagaag ttgttctgaa
     1141 agaattaaga gaggaacgta ttcccgttgt catctattat cacggatggc agacaagtaa
     1201 ggagttagtt ttgacgcaag gaagaaaaat tgcgcagcag ggcattcgtg tgatcctgcc
     1261 agatgcgatg aatcatgggg agcgcaaaca gccggtttct tctattccat cctatacatt
     1321 ttgggatagt atctacggaa atctgtttga atttgacaca cttatcaagc atttagaaaa
     1381 aagaggtctt ctacatgatc agcttgcagt tggcggggtt tcgatggggg gaatgacaac
     1441 atgcgccttg ttggctaaac atcctcatat agtagcgggc gtgtgtttga tgggttcccc
     1501 tgcacctttg gaatatggaa aagaaatcgt aaggcgcgca tcggaatata attatgtcgt
     1561 accaaaagac tattttgatt tgatttcatg ggttaagagc tatgatttgt cattgcagcc
     1621 ggaaaagtta gctggacgtc ctctgttctt ttggcatgga caagctgacg aaaaaatccc
     1681 ttatatacaa acggaacgat ttgtagaaaa aaatcggcat aagtcttacg gaaaagaaat
     1741 tgtttttcgt tcctcaaaaa cacagcgaca tatggtacaa ataccactaa tggaagaagc
     1801 ttccactttt ttagcaaaaa atttgataaa aaagtataaa taattgaaac ggaaaatcac
     1861 tcatgacgat agaggatttt ccgtttttta ttttgggcta taataaactc tgttaggaaa
     1921 atttggtata attgatagta agaatgacaa catgttagaa aggaagagag catgcccact
     1981 ttagcagatg ttgcaaaacg tgccaatgtt tcaaaaatga cggtctctcg agtcatcaat
     2041 catccggaac aggtgactga cgaattgaaa gagttagttt tctcagcaat ggctgaactg
     2101 gattaccgtc caaatattgc tgcaaaagca cttgtcacga atcggtcgca gatcatcaaa
     2161 ttatttatac tggaagaaat cgacacaact gagccctatt acatgaattt gctgatggga
     2221 atcgctaaac ggatcggtaa agcgcattat tctctccaat tagttacaaa tgatgccttc
     2281 gacgtcggtt cttgtgatgg atacatcatc acaggtgtca gaaaaaaaga cttcgattgg
     2341 atcgaccgtt tggaaaaacc agttgtcttg tttggggaaa attctgaagg atatgattat
     2401 gtagacagcg ataatgaata tggtacagca aaagcaacgc aatatgcctt agaaagagat
     2461 tatcaaacga ttatttatat cggtatcgaa gtggacgagc cttttgaaat ttcaagggaa
     2521 actggttatt tatctgtgat gaaggaagtt gatcttccta gtaaaatctt gcggtttcca
     2581 aatcattcga cagaagtaga aagatatatg acagaaaact gggcagattt tacagataaa
     2641 acttgttttg tatgtagttc agatcggcta gcactaggag cagtaagagg gattataaat
     2701 gctggaggac acttgcccga agaatttggt gtgattggat ttgatggggt atttttagac
     2761 caaatcagtt cacctaaatt gacaacgatc aagcaagata ttatacgtat gggtgaagta
     2821 tgtggagaaa tgttgttaaa aaaaatagaa gaaaaagggg cgagtcaagg ttatcgtcgt
     2881 ttttttcctg aactagttat cagagagaca acgaattaag tcttattact atatatatat
     2941 ttatgaaatt catcgctatt ttcttaaaat aatcagcgca ttgctactga aaacaatgaa
     3001 tctgtgctat tattgagaaa agttccattt ttgagaagga tggtttgaat gaaagaaatc
     3061 agacgaaaac atttccgttt tcctgcgtac gatgatgagt taggcgtaaa attaaccaac
     3121 gacaagaagc gagtattatt tgacggacaa gaagatcttt tgactgaaca gaatcagtcc
     3181 ccgtttgaac agatgaaaga atttcatttc acgaacacga acaccgagaa aatccgtcgc
     3241 aagcgtcgtg caacaaatgg attgaagtca gctccgcgta aggaaaattt ggaaaagcat
     3301 aaaggaaaat taccggatta taccggccat taccaatcag aaatcacatc gacaggacga
     3361 aaaaaacttt ttggtgattc ctctcgttct gtcagacaaa gtgaaacaag gacagagtcc
     3421 aagcatacta cacgaaacgc atctgttgac acgaaggtgc gggagcgttc ttattttgtt
     3481 cctaaatatg tacccgcatc gattatccca gaccctagag aaagcaaaat ttctgaggat
     3541 catttaatcg aatcgatgaa aaaaacggat gactcttatc tgttgttcga tacagaaccg
     3601 acgccgtatc aagaaaaaag aaatggtgag ccgtctgtac gaacattcaa tcgaacaaaa
     3661 acgttagagg aaacaaagca gtcagaagaa aaaaaatcga aaaaaagcca tggtatctta
     3721 gaacgctcat tacaaggatt gatcgatgat caaggaaact ctttgggaga aaatagctat
     3781 tttaaataag tgatatacaa atttatattc ggctacagtt ggaaaggaaa cgctgcggcg
     3841 tttccttatt ttatactcta tccgaaagtt tatatcgtat cctcaaattt ttttgagttt
     3901 aagcgaattt tttctcccaa taagtaagaa aataatggca aaataaaaaa acaaactatt
     3961 ttcgcagatc gtttgcaaaa atagtt
//
LOCUS       DARBFD010000154         3949 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000154 DARBFD010000000
VERSION     DARBFD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.160"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(597..1154)
                     /locus_tag="SCK08_002724"
     CDS             complement(597..1154)
                     /locus_tag="SCK08_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202550.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1333..1944)
                     /locus_tag="SCK08_002725"
     CDS             complement(1333..1944)
                     /locus_tag="SCK08_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202551.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2057..2314)
                     /locus_tag="SCK08_002726"
     CDS             complement(2057..2314)
                     /locus_tag="SCK08_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202552.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2911..3189)
                     /locus_tag="SCK08_002727"
     CDS             complement(2911..3189)
                     /locus_tag="SCK08_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202553.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3223..3474)
                     /locus_tag="SCK08_002728"
     CDS             complement(3223..3474)
                     /locus_tag="SCK08_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202554.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1199 a          809 c          523 g         1418 t
ORIGIN      
        1 ttaaacgctt ctgtcccagc ctccttattc cgaataaatg gtgtccaaaa gcttgcatct
       61 gccagattat ctcatttctt atgacaagct aagcaaatta tctccaccaa ataatttatt
      121 catatttttg acttgcgcat actaactaat tcttaaattt tcatacaaaa tttctcaatt
      181 tcccttaacg agtaatattt atgtacggtg attctcacta gtatcccaat cactttttaa
      241 gttatcccga tttcaattct acttcaaaaa tagttgcttt tatgtatgct gttagttgat
      301 tagatggtca tttgttgagc taggtcgttt attttaaaca agttgtttct ttagcttata
      361 cctatctcgt ctaccaaacg caagatggca aacacaaatg catacgcatc tgtatttgcc
      421 actttgattc gtataaagct aacaactatt aactcctctc agctaatagt tgctctcttt
      481 tttattcaga ttcatttttg ctgatcacag cctagtattt caatggtttt gaacactaaa
      541 ccaaattgtt ccattacaac attcactggc tgatgaaatg gattagtaga tttcgattat
      601 tcaaagcgat tttctattgg tggatcgtgt tcccaactaa taagacggat tccaaaccta
      661 tcgtacagat cttctaaacc acttataaat tttgcaccta tttcaggtgt tttattgcgt
      721 ggatattcta ttgttacgcc attaaatgga taccaattag gattaggctc tctttttttc
      781 tgtacatctc ccatagaata gttagcactt tctgggacat aatatattct tcgaccatcc
      841 tctaattcat ctaataagat atttcgtgat cgtctatcac ttgctaaatg tagacgccac
      901 gtgcgatacc atgccatgct acctgcatct gcagaaaatc catcaaataa tgcaacaatt
      961 gtatcttcta tcacaatcct ttccccatct acttcatatt ctaatctaaa atcaaactcc
     1021 cctcgggtaa tttctggtct tggtggctta ggacccaata ttattctact taaaaccgca
     1081 ttcgttccca gatgaaacac agctataaca acaataatga ttaatatttt gtttctattt
     1141 gataaatttc tcattattcg cacatcacct ctacattctc acatattagt tcgtaatact
     1201 cctttaatat caaaatatct actggtcgta ataatattcg ctatgaattc ctactactcg
     1261 tataagaaag gccaaatcat cagcttttac ttgctgatga tttggcttgt ttctatttat
     1321 cgtaattgtt ttctaatcac caaaattgtt aacaatcggt tcagatattt cccagttgat
     1381 taaggtaatt ccgtattgtg tcaaaacttc ttgcgcttct ccgaaattaa gtacagttct
     1441 tatgtatcca ctttctccta atataaagtc tacctctctt ttaaaacctt cctcgagatt
     1501 tctaagggct atatgtggtt ttaatgacat tgattgtaca ttttccccca tataatattg
     1561 cgcacttcct cgactaaaat agattcctag atcgtcccca aaaggtagat cttctctacc
     1621 actagctaat ctacttctcc atcttcgttc tcttttcata gaacctgcgc tgaactcaca
     1681 cccgttaaac tcagctatga cagtatcttc aattatgtta atctgtccat ctagttcgta
     1741 ttcaatccta aatggaaatt ctccataggt gatttccggt tcgggtattt taggtgagat
     1801 taatgcaaag aaaggaaata ttaagcttgg caacatcaaa aaaactcctc ctatgatcac
     1861 aattcccgat gtaatccaaa atataatttg tacaattctc attgatccat tgtattccat
     1921 tttattcccc ctattactat tcatatccgc ctatctatat cgatcttcca atgcagagat
     1981 aattgcttcc ataccttgat tttttactga gttacctatg cgagatatca ttctccacta
     2041 aggtgcatca tattgtctat ctaacatgat taattctcca ataggtgtat actcctccaa
     2101 aaacaacatt taatgcatct ccctcgacaa cataagctac catgtttgct gtatatgtgt
     2161 ttgagtctaa ttcataagca gataaattta ctgtcgctgg attaaatata atcgcattcg
     2221 tatttatagc tactgaattt gctgcttcag caccaccttt tgaatgtccg atcattgtga
     2281 tttcaatagt aggatttacg tggacagccc tcaccgttta ccctgaccat tatagaaatc
     2341 gcttactttt ttgaactacg caacaaagga tacaatgctc ctaacttcac tgtctccatt
     2401 tttgtccact aaggaatata aaaaataggc attaattcat ttaattcggt cacaacacgt
     2461 taccttttaa atcattatac tgtatctcat acattattat caataaacta ttataaaaat
     2521 gcaaaaaatg ctttatcaat ctattttgta attggtcata atatcattga aatcataccc
     2581 tgtatcgatt tttcctatga atctcgtcta tttgtgattg acgaaaaaga ggcaagacag
     2641 acactggaat ggtaatttta cccactattt tgctccaact atttgtaatc gtacccactt
     2701 ttgaaatacc gagatgatca ttttaattga tatgctgatt cccaagcaaa accccaagaa
     2761 attaaatgct ctatcgcatc tagcttcttg ggttttgctt atataaattc caacatttaa
     2821 attttttatg ctgttcggtc tcagaaatgc cgtttttgct tatatcacaa tatcaagatg
     2881 tcaaattatt cgtgttgccg atgactttga ttaatttttt tcaaattgtt tattaagact
     2941 aatgctgtct ttttgcagat accaatgttc gaacttttta acattatcat atgatcctgc
     3001 aagttttaac ttcttaccat tagatgttat caaagttata attaaccttg cctttttccc
     3061 attaggatct tttattttat gaattttctg caggtaatct ctcctaatgg cagctatttt
     3121 tacctcatca gtgctgataa accattgatc atccacatac atactgcctg ttatgttgtg
     3181 tttttccatt tcttccgaga acgaaaagtt taaggctcgt tcttacttcc aaagttgaat
     3241 gagtccatgg attggactta gtgtgagaat tgagaacaac aataagaatc catatcctcc
     3301 cgcaaacatc cctaaggcta tactgtctct ccatccataa gtaggattag aagagaaaag
     3361 agtcaccgtt tgtaataata tgaaaaggaa taatccagca caaacaccaa agataacaac
     3421 tccaaacatt gaatttttat acaatgacca tttatttata ggcttttctt tcattttact
     3481 tccccctact gttctgttca cacggaatga attcaaagtt cataagccct cctctttgaa
     3541 catatgaata cgacttgcta actatacttt aaaaaagctt gttttaagcg gttataggcg
     3601 agttgtaatc tctattcatc tcattagcaa ataacttgcc gaaaagtcta ctgaaattaa
     3661 agcagatagt ggaagaatta tttagccagg atagacacta tttgtgaaac acatgtcttt
     3721 acttttgaaa ttctatgatg aaaagccttg gaatacagta gctcttaatt ggtaatactt
     3781 tttccattat aaataacctc ttttcatttt atatttatcc cctctaaaaa taatatctaa
     3841 tctaatattt taaacttata tttccctcgc tgtgctaata gagcattact aaataaatag
     3901 aaactagaaa aaagcattac gcctgccaga ataaaccaat tatttgtat
//
LOCUS       DARBFD010000155         3907 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000155 DARBFD010000000
VERSION     DARBFD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.161"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1432
                     /locus_tag="SCK08_002729"
     CDS             <1..1432
                     /locus_tag="SCK08_002729"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1202555.1"
                     /translation="EYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFN
                     VLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEV
                     IKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQK
                     ERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAE
                     EMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSK
                     ADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLF
                     FGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVE
                     KYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLE
                     AISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1422..2639
                     /locus_tag="SCK08_002730"
     CDS             1422..2639
                     /locus_tag="SCK08_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1202556.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2652..>3907
                     /locus_tag="SCK08_002731"
     CDS             2652..>3907
                     /locus_tag="SCK08_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1202557.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1353 a          669 c          814 g         1071 t
ORIGIN      
        1 ggaatatgac acagtaagca aacaaacaat gttgtatcgt gaattacttt cagatgaaga
       61 atcaaaagaa gatctgattg ccacaatcgt tgatattttg ggatatgcaa ttgcaccaga
      121 gtatcttttc aacgtcttag cggatcaagc aaaacaagca acgttccaat tgaacgatct
      181 aaacaaagcc tttgtgcaat tggcatctac ctacaatcaa tttaatgggt tatttgatga
      241 tgtggatctc caatcaaaaa aactaggaac agatgagcaa caacgaaatg tgacgattac
      301 cgaagtcatc aagaaattga atgatgtaga tgttttagaa cacgatgggg acgtgatcgg
      361 ggatgcgtat gagtttctga tcagtcaatt tgcttcagaa gcagggaaga aagccggaga
      421 attttacaca cctcatatgg tttctgatat gatggcgcaa atcgtaacgc tcgatcaaaa
      481 ggaacgacga ttctttagtg tttttgatcc aacgatgggt tcaggttcat tgatgttaaa
      541 cgtacgtaac tacttaacac acccagataa cgttaaatat catgggcaag aactaaatac
      601 aacgacatac aatctagcaa aaatgaactt gattttacat ggtgtcgacg ccgaagaaat
      661 gaatctccgc aatggggata cgttgaataa ggactggcca acagatgaac cttacacatt
      721 tgatgcagta gtaatgaacc caccttattc ggctaattgg tcagcagata cgactttctt
      781 agatgattct cggtttaatc gttatgggaa attagcgcca aaatccaaag cagattttgc
      841 gtttcttctt catgggttct atcatttgaa ggaaacaggt accatggcca tcgtcttacc
      901 acacggagtt ttattccgtg gcgctgcaga aggggtcatc cgtcaaaaat tactagaaga
      961 tggcagtatt tatgctgtga tcggtatgcc tgcgaacttg ttctttggga catcgattcc
     1021 aacgacggtg attgtgttga agaagaatcg tcaaactcga gatgtattat tcattgatgc
     1081 tagtcgtgaa tttgttaaag ggaaaaatca aaataaacta tcggaagaaa acattcaaaa
     1141 aatcctcgaa acctacgctg aaagaaaaga tgtcgagaaa tacgctcatt tggcaacctt
     1201 tgatgaaatc aaagaaaatg actataactt aaatatccca cgctatgtag atactttcga
     1261 agaagaagaa ccaattgata tggttcatgt cggaaatgac atcaagaaga tacgacaaga
     1321 acagcaagtg ctagaaaaaa aacttttaga agctatctct tccctgcaaa caacacctga
     1381 aaatgaagcg tggttacaag gtgcgctgga ggtgttcaaa catgagcaat gatacacaac
     1441 cagaaatccg tttcccagga tttacagatg attgggaaca gaggaagttg ggggacttcg
     1501 ttgtagatta tgttgaaaag acaagtgtac aaaatcaatt tcctatgcta acttcatctc
     1561 aacaaaaagg gatagttttg caagaagact attttgcaaa tcggcaagta actacagaaa
     1621 ataatattgg ttactttgta ctacctagag gatatttcac gttccgtagt cgaagtgata
     1681 atgatgtgtt tgtgtttaac cgtaatgaca ttattgatag agggattata agctattttt
     1741 atcctgtatt tactttaaaa tctgctgatt ccgacttctt cttaagacgt attaacaatg
     1801 gaatccaacg acaattatct attcaagcag agggaacagg tcaacatgtt ctgtctctaa
     1861 aaaagtttaa aaatatagta gcaatgtttc ctagtgagga agaacaacaa aaaatcggca
     1921 ccttcttcaa acaacttgac gacacgatcg ctcttcatca gcgtaagtta gatttgctga
     1981 aggaaacgaa aaaaggattc ttacaaaaaa tgttccctga aaacggagca aaagtgccag
     2041 aaatccggtt tcctggtttt acggaagatt gggaagagcg taagttggga gaattaggta
     2101 aaactcaatc gggaattgga tttcctgatg ccgagcaagg tggttctgaa ggtactccat
     2161 tttttaaagt ttccgacatg aataacattg gtaatgagta tgaaatgaga aatgcaaatc
     2221 attatgtatc taatgagcaa attgagcgta aaaaatggaa gccaataaaa gatgtgccag
     2281 cgattatatt tgctaaagtt ggcgcagcaa taatgcttaa tagaaaacgt ttggtcacaa
     2341 gccctttctt aatagataac aataccatgg cttatttatt taataatact tgggatatct
     2401 attttggtaa aatattgttt gaaactataa atttgccaag atactcccaa gtaggggcat
     2461 tgccttctta taacagttct gatattgaga atatcagtgt taaggttcct gtaaaggatg
     2521 aacaacaaaa aatcggtact ttcttcaaac agctagatga tacgatcgct cttcatcaac
     2581 gtaagttaga tttactaaaa gaaacgaaaa aaagcttctt acaaaagatg tttgtctagg
     2641 agggaagaag aatgaaaatt agtcaccgtg aggaagcaga agtcgaagaa cagttgattc
     2701 gtgtacttgg agaagggcat aatcagtgga cgtatcgtcc ggatctaaaa tcagaagaag
     2761 acctttgggt caatttacgc caaaaaatta tctcaaataa tcaagcagaa ctgaatgatt
     2821 ttcctttgac cgataaagaa tttgaaacga tcaaaacgga attactgctg cgaacgaaaa
     2881 ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg atcacgattg
     2941 aacgggaaga ttcgcaattg ggttctgtct cacttatcct ttattccaat caagacattg
     3001 gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag
     3061 aagcacggga tcgacgtttt gacgtcacct tattaatcaa tggattaccg attgtccaaa
     3121 tcgagctgaa acaagtaact gcaaaagatg gtgtttatca ggcatttaac caaatcaaaa
     3181 aatatgcaga agaaggtatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg
     3241 tttccaatga acaaaccact cggtattttg caaatgcctt accgaaagac ttgcacccta
     3301 agtttttatt tagttggcga acgaaagaca atgaaaaagt agaaaactta tatgagtttt
     3361 gtaaacaagt gttaaatatc ccggatgctc atcgtttgat tgcggattat acaatcgtca
     3421 gtgaagatca agacaataaa accctcatgg tgttacatcc ttatcaagta catgcgattc
     3481 aagccttgtt tattgccgca aataaacatc aatcagggta tgtgtggcat gcgactggtt
     3541 caggaaagac gttgactagt tttgtatcta ctaagctttt agcacgaaaa tcggggatcg
     3601 atcgaacgat catgttagtg gaccgcaaag atttagataa tcaaaccacc acagagttta
     3661 caaaatttgc ttcggaattt aatactggga tttcttccgg gaacgccaaa gcgaatagct
     3721 tgatcgttgg aacgggaagt gccaaagaat taagtgaaac cctgttggca gatgccaatg
     3781 caaatgttgt aatcattaca acccgtcaaa aactagatgc ggcgttgaaa tatgcaaaga
     3841 aacaagaaga gaaaaaagga accaatcgct tccaaaaatt aatgggccaa catatcgtgt
     3901 ttgtagt
//
LOCUS       DARBFD010000156         3876 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000156 DARBFD010000000
VERSION     DARBFD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.162"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            158..2257
                     /locus_tag="SCK08_002732"
     CDS             158..2257
                     /locus_tag="SCK08_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1202558.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="SCK08_002733"
     CDS             2453..3316
                     /locus_tag="SCK08_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1202559.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="SCK08_002734"
     CDS             3421..>3876
                     /locus_tag="SCK08_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1202560.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DARBFD010000157         3713 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000157 DARBFD010000000
VERSION     DARBFD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.163"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="SCK08_002735"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="SCK08_002735"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEG1202561.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="SCK08_002736"
     CDS             complement(2261..2512)
                     /locus_tag="SCK08_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEG1202562.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="SCK08_002737"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="SCK08_002737"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEG1202563.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="SCK08_002738"
     CDS             3462..3680
                     /locus_tag="SCK08_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202564.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DARBFD010000158         3567 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000158 DARBFD010000000
VERSION     DARBFD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.164"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            155..2344
                     /locus_tag="SCK08_002739"
     CDS             155..2344
                     /locus_tag="SCK08_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1202565.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2341..>3567
                     /locus_tag="SCK08_002740"
     CDS             2341..>3567
                     /locus_tag="SCK08_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1202566.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         1350 a          481 c          593 g         1143 t
ORIGIN      
        1 agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt
       61 attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa cttactatat
      121 aatcatatta attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg
      181 aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat
      241 aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga
      301 tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa
      361 tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga
      421 atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga
      481 agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa
      541 caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa
      601 cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa
      661 tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat
      721 agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga
      781 tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat
      841 gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc
      901 agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt
      961 tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc
     1021 agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc
     1081 aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca
     1141 tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga
     1201 actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa
     1261 aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca
     1321 ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa
     1381 tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt
     1441 aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa
     1501 tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat
     1561 tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat
     1621 taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca
     1681 gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt
     1741 gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt
     1801 taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt
     1861 agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa
     1921 agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag
     1981 aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt
     2041 aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt
     2101 tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga
     2161 cttgtacgag gaaactacag aattgtcagc tttatcagct agtttaaaaa caagtgatac
     2221 agctgatttg aatatagcaa taatttcttt attagagtac tttggtaaag atgaggatgt
     2281 tgtgtatcta atcaatcaga tagaaaatta cacaaaaata ctttatgctg aggtttcaaa
     2341 atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa agatgttgtg
     2401 gaaagtgaag ataaccttta tgtaaatgca tgtcctggga gtgggaaaac aagggtactt
     2461 acaaggaaaa ttgcttacca agcaattaaa cacgatggat cactaaaaag aataattgct
     2521 atcacataca ctaatagagc tgctgaagaa attaaggaac gcctaagttt acttagtata
     2581 gatgatgata ttaatatttg ggtggggaca atacatcagt tttgtcttga atttattatt
     2641 tatcctttta aaatgaatct ctcaagagta tcaaaaggat ttacaattat tgatgattat
     2701 actcaaagag cttacgtcag ccaaattaat aatatgctga atttaaatct caaaacgtat
     2761 gaactgaaca aaattgattt gtcactttct catgatttta aaattaatga agttcagtat
     2821 actcaagttt ctgaaatgta tcacactatg ttggaaaaaa ataaagaaat tgattttgat
     2881 atgattttac tagtagcgta taaaatttta actgtaaaca gtaacgttgc acgtaatatt
     2941 gctagtaata taagggcgat ttatgtggat gaatttcaag atactagaga attacaatac
     3001 aatgtaatag ctaaattact tcaaaacaac cctcaaattc agtcaatgtt tgttggagat
     3061 attgaccaag caatttatgg atcattagat ggtatagcta aatcagttga agaactagaa
     3121 atgctaacta gtcacacttt tcaatcaaag acgctacatg gctgttacag gtctaatcag
     3181 agattagtag acttctactc taattttcag agttgtcctt acgagattga aagcagaggg
     3241 aataataaaa acgaccgtgg ttttataagt tatgattgca aagttactaa ggaggattta
     3301 cccaacataa taaaaaatat aataaaggaa aagattgaaa gcggcattca tgaaaaagat
     3361 atttgtgtac ttgctcctca acactatcct ctgttttcac tgggtgagta tttaaagaaa
     3421 gaattaccgt attgcaattt tgatgctata aatatatcac caattaaggt aaataatcat
     3481 agtttatttt tcaagttagc aattttgtac tttactgaat caggtgaaaa ggttcgaaga
     3541 agaaagctaa ttgcaaacga tatttta
//
LOCUS       DARBFD010000159         3437 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000159 DARBFD010000000
VERSION     DARBFD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.165"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="SCK08_002741"
     CDS             complement(<1..2177)
                     /locus_tag="SCK08_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202567.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="SCK08_002742"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="SCK08_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          975 a          758 c          538 g         1166 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgaatcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acacttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattaccaa ataacaa
//
LOCUS       DARBFD010000160         3381 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000160 DARBFD010000000
VERSION     DARBFD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.166"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..798
                     /locus_tag="SCK08_002743"
     CDS             <1..798
                     /locus_tag="SCK08_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEG1202568.1"
                     /translation="FDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGG
                     IYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVM
                     VVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVAS
                     AIVNILLNMIFIPRLGIIAAGYTTVFTYFLLFIFHYNIAKKITNKQLFDTKKICIIII
                     FVSIFSLILHILINQMLIRYVLTSLLIISLVIYFLKIKNRIIDDYNS"
     gene            811..1818
                     /locus_tag="SCK08_002744"
     CDS             811..1818
                     /locus_tag="SCK08_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1202569.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1830..2834
                     /gene="neuB"
                     /locus_tag="SCK08_002745"
     CDS             1830..2834
                     /gene="neuB"
                     /locus_tag="SCK08_002745"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEG1202570.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2831..>3381
                     /gene="neuC"
                     /locus_tag="SCK08_002746"
     CDS             2831..>3381
                     /gene="neuC"
                     /locus_tag="SCK08_002746"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEG1202571.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNR"
BASE COUNT         1212 a          431 c          640 g         1098 t
ORIGIN      
        1 tttgataaaa aacagtggag atacgcttta aaaatctctt tgccgctaat cccacattcc
       61 ttagcaaata tagttttagc acaattcgat agaattatga ttaattctta tttaggtcct
      121 gctgcggggg gaatatattc ctacattagt aacataggta ttattctaag cgtaatatgg
      181 atgtctacta ataacgcctg ggttccatgg ttctatggtg agatgagtaa aaataatgaa
      241 aaaaaagtga agaaagttag taactattat ttagtagtct ttactttgat tacgatgatt
      301 gtaatggtag tttcgattga tttagcaaaa tttatggctc cgaaagacta ttattctggg
      361 ttatctttag tggttccgat aaccttaggc tatttctttc agtttttata tagtttacca
      421 gttaatgctg aattttatga gaaaaaaaca tcgtttattg cgattggtac agtagcaagt
      481 gctatagtta atatactatt aaacatgatt ttcataccta ggttaggaat aattgctgct
      541 ggatacacaa ccgtatttac gtatttttta ctttttattt ttcattataa tatcgctaaa
      601 aaaattacga ataaacaatt atttgacaca aaaaaaatat gtatcataat aatttttgta
      661 tcaatttttt ctttgatact tcatatttta atcaatcaaa tgttgattcg ttacgttttg
      721 acatcattat tgataattag tttagttata tattttttaa agattaagaa tcgaattata
      781 gatgactata attcatagga ggagatataa gtgtttaaag gaaaaacaat attagtaact
      841 ggcggaactg gttccattgg aagtgaaatt gttcgtcagc ttttaaaata tgaaccaaaa
      901 gctatcagaa tttatagtag aggtgaagaa aaacaatttt atatgcaaca agagctctca
      961 aatcacacta atttgagatt tttaattggt gatatcagag actctcaaag gattgattat
     1021 gcatgtcgtg atgtggatat tatttttcat gctgccgcaa tgaaacacgt tccagcaagt
     1081 gaatataatc ctatggaagc tgtaaaaaca aatgtgatgg gaactcaaaa tgtaatagat
     1141 gcagcgatca aaaataatgt aaaacatttt gtaggtatca gtacagataa agtagtgagt
     1201 cctactaaca ccatgggtgc gacaaagtta ttgagtgaaa aattaataat agctgccaat
     1261 cagtataaag gaaaaacaga aacaatattt tcttgtgtaa ggtttggaaa tgtcatgggg
     1321 tcaagtggat ctgttattcc tctttttagt aaacaaataa aaaacaataa agatattact
     1381 ttgacagatg ttgatatgac acgatttatg atgtcaatac cggatgctgc aagattagtt
     1441 ctgtctgctg ctatgaattc aaaatttgga gaaacatatg tcttaaaaat gccggcaatc
     1501 aatattttaa ctttagctca atcattaata aaaatatata atgatacaca tgatgaaaaa
     1561 tatagaggtg aaataaaagt tgtaggtatc aggccaggag aaaaattata tgaagagcta
     1621 atgactacag acgaaattga acgctcatat gaaaatgata aaatgtatat cattccacca
     1681 cactatcatg ctactgacga actgtacaaa gattatgaaa aaattaaaat cactgcatac
     1741 tcttcagatg caacagaatt aatgagtgga caggaattta ttagaatcgc aaaagagaat
     1801 aatgtcgtga cttggtaagg agttttatga tgaaatcaga agtatatgta attgcagaaa
     1861 ttggtgtaaa ccataatgga agtctagatt tagctttaga aagtattgat aaagctaaag
     1921 aatgtggagc taacgcggtt aaatttcaaa catttaaaac gaataagcta gtttccaaaa
     1981 aggctccaat ggcaaaatat cagaaggata atcttaaaga ggacagttct caatttgaaa
     2041 tgctaaaaaa attagaatta tctcaatctg attttattag aataaaagat tattgtatac
     2101 aaaataaaat tgattttttg tctactcctt ttgatgaaga aagtgcagac tttctaaaac
     2161 aaattggtgt acaaggattt aaaattggct caggagattt aacaaattta ccttttctta
     2221 gaaaaataga tatgtatggg ttgccagttt tattatctac aggtatggct gatattcatg
     2281 aggtcagtga ggctgtagat tgtttcatag atagtccagt tactatttta cattgtactt
     2341 caaattatcc ggctagttct gaagatatta atttattggc gattaacaca ttaaaagaaa
     2401 tgttcgggat acccgttggg tactccgatc attcattggg atatgatgtt ggtatttgtg
     2461 ctgttgcgat gggtgctaag gtaattgaga aacattttac cttggataag gaactacctg
     2521 gtccagatca taaagcttct ttagatcctc aggaatttgc agattttgta aagcatatta
     2581 gaaacactga aatattttta ggtgatggtg ttaagcgagc aatgccttct gaacttagta
     2641 caaaagaagt atcaagaaaa agcctagtgg tcactgagaa tttaaatgtg ggagatattc
     2701 ttacagagaa atctcttaca ataaaacgtc ctggaaatgg aatagctcct aagtttttat
     2761 atcaatatat tggcaaaaaa gttaaaagac caataagtgc agatagtgtt ctgcttgagg
     2821 aagatgttga atgaaaaaaa tacttatagt aagtggaaca cgagctgatt atggaatata
     2881 taaacctgtt gctgagaatt tactacaaga taattgggat gtatcatttg ttgtttctgg
     2941 aatgcattta tcccataaat atggatttac taaagaattg atcttagctg acggatttag
     3001 aattagtgga gaagttcaaa gtttgttttt agagaacaat aagggaaaca tggctcggtc
     3061 tgtggcatta gagataaatg gtttcacaaa tatatttgaa caaactagcc ctgattttgt
     3121 attattacta ggggatagag gagaaatgtt ggcagctgcg atagcttgtt tgtatcttgg
     3181 aatcaaaaca gcacatattc acggaggcga agtgtctggt actgtagatg aatcaataag
     3241 acatgccata acgaaaatag catctgttca ttttgctgct acggaaaaaa gcaaagagcg
     3301 tttaataaaa atgggggaag attcttggag agtttttccc gtcggagctc ctagaataga
     3361 tactatttta aataatagat t
//
LOCUS       DARBFD010000161         3277 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000161 DARBFD010000000
VERSION     DARBFD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.167"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(88..1035)
                     /locus_tag="SCK08_002747"
     CDS             complement(88..1035)
                     /locus_tag="SCK08_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEG1202572.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
     gene            complement(1040..1792)
                     /locus_tag="SCK08_002748"
     CDS             complement(1040..1792)
                     /locus_tag="SCK08_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1202573.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            complement(1793..2701)
                     /locus_tag="SCK08_002749"
     CDS             complement(1793..2701)
                     /locus_tag="SCK08_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEG1202574.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(2701..>3277)
                     /locus_tag="SCK08_002750"
     CDS             complement(2701..>3277)
                     /locus_tag="SCK08_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1202575.1"
                     /translation="NKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIEL
                     PWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNL
                     TIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKE
                     FFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT         1077 a          613 c          406 g         1181 t
ORIGIN      
        1 ttctatccca gatttgattt tacaaaactc atcagtcctt attgacaaag agcctgaatt
       61 taccctctaa tcttatctta gtatgtttta attgatttca gttttcaaat aattagtcat
      121 tccacttaaa ataccatatt tcggttgata gccaattgac tggatcttag taatatctgc
      181 tagagagtca tggatatcac ccgaacgctc ttctttgtat tcaagtgtca attctacacc
      241 gagaatttca ttgattgcat gaatcaattc caaaagtgtg gttgctttac ccgttccaac
      301 attgaattgt ttccccagtg cagcttcctc attcgcaact aaaagtaatg cttggattac
      361 atcatctata taaacaaagt ctctagactg gcttccatca ccaaataatg taaatgaagt
      421 tgcttctcct acgatttgtt tcttatagcg atctacgaga atagaaatca caccactgta
      481 tggagaattc ggattttgat ttggaccata gacattgaaa aaacgaactg cgctaccggg
      541 tacatcatat aaatgacaat aatctagcac atattgttct gcagcgaatt tatcaaccgc
      601 ataaggcgtc aatgggcgaa tcacggactc ttcttttttt ggtaaagtcg gttcatcccc
      661 ataaacagcc gctgaagaac taaacacgat ccgttttaaa tcaggttgat acttgcggat
      721 caattctaaa atcattaata cactttcaaa attcacacga tgtgtttcta ctggtcgggc
      781 aactgaatct gccacactag caactgctgc taaatgaaag atataatcaa atttattagc
      841 tttcatgatc tcttccatca aggatttctc agcgacatct ccctcaataa aagtgatatt
      901 tttcgtcata tcaagatttt ctacttttcc cattgataag tcatcgacga cgattaccac
      961 atgttcttct cctaaatgat tggctaaggt agaaccaatg aagccagccc caccggtgat
     1021 aagataacag ctcatccttt tacctccgat ataaataatt atagatatct tgaagctccg
     1081 aagaatcttc taaaaattta ggaaacaaaa attctaacca tttaaaatct tcttcactat
     1141 caatatctaa aattagataa tctctcattt ttattatccc acattgccca tcaaaaatat
     1201 aattactttt ttttagaaat tcaggtttaa aagaatacat tgaggcattc atttcaaaaa
     1261 cttcaggtgt ctgttgccta gatgtgtatc ttgatttatt aactatagta acttcatttt
     1321 caattctttt taccatatta aaatagggat tacgtcttgc agaaacaact gaaaagtaga
     1381 cgtctttatc taaatttttt agatgttcat ctattaattc ttcaagatta gaaacttcac
     1441 gtaaaggtga agtcaaatct aaatctataa ttaaatcaaa tatattatct tctctttcta
     1501 gttgtaagaa tgaatcacgt atcacttcaa ttttccctac agtatctgta gctaattcag
     1561 gtttacgttc tacaattttc acagacggat ttaacttagt cgtcaattct actaactcat
     1621 tactgtccgt attcaaacaa gtcgtgcaat tatgttcagg atgtttttgt tgatagatat
     1681 ctatcattgc taaagcgaaa tggactaggg gctttcctgc caaatatttc aaatttttat
     1741 ttttaaaccc cttagaccca gctctaccac aaatagtaaa taaaatttcc atttatttct
     1801 cctcagctat tttcaatact tcgtttgcat gatataaatc attccagttt tctttgcctg
     1861 ttactacgtt ttcaaagaaa tatttcaact ccttctcata catatcatta gcagattcag
     1921 gcatttcttt aacaatgttt tcttcaaaac ttattttatt atttaataag tcaacattga
     1981 tacattcatc taaagtaaac aattgaaatt ttcttgtagg aactcttcca aaataatcta
     2041 aatgaatttc tataatttta tcatcgtatc tcaatatata tgttgctaaa tcattactgt
     2101 taatttcaag attcgaatat ttaccaaact gtttttttac tgatttaggg aaaccaaata
     2161 ggtttataat ataatccaat tcatgtatac agtctaattc tactcctccc cccatagatt
     2221 taaaggcaga ataattattt ctataatcag taccttttct ccaatcagga agataagtag
     2281 aacaaatagc tcttacagaa tatacttttt tactttgaat actattacga acaacttcca
     2341 tgatattttt atacctcagt ggagcagcaa cataaatatt tttgtaatca taattatatt
     2401 gagtaagatc aatactctca gaaaaagcag gtttttctaa aaatatgtaa tcaacttttt
     2461 caacaattac atttagtgtt tccagatgct taaaagtcgg atttgtaata aaagctatat
     2521 cataatgtgt atccaaatcg tctctatttt gaatatacgt tatttcttca aactcttttt
     2581 ttccactgct actataagct gttatatcaa aattaataga attctttttt ccatagctta
     2641 ttagattttt tagatgtctc tttccaattg accctaaccc aaaaaaaatt actttcatca
     2701 ttaatttacc tcaatgtcgt taattaagtt aataatatac ttatcttttt caaaagtata
     2761 ttggggttcc attttctttt ccaaaagcgt gaaaaattct ttaatctcct ctaaatatgc
     2821 atcttcaata atagtatgtg aataattatt atcatgcata acctcatcgt acaattgaat
     2881 atctgtcata ctagaagttt cttcattcca tgaaaataga gaatccgggt taccttccca
     2941 ttctattaaa atcttttcac caattatagt taaatttctt ttagctactc ttgaaacaac
     3001 atctacactt aaagttccaa ctataccagt ttcatgctca ataataagag tgaaactgtc
     3061 cgaataatca atatcaagat tggataattt ctgggtcatt acagtaaacg atttaacttt
     3121 cccaaatgta tctataatcc aaggtaattc aatagcgaat atttctctac acccattagt
     3181 ttttttgttt gaaacaaaaa aattgcgata atcttcccaa ggatgccagt cgggtaaata
     3241 ctgtcctaca tggtattgat aagaaatttt tttatta
//
LOCUS       DARBFD010000162         3232 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000162 DARBFD010000000
VERSION     DARBFD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.168"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            157..507
                     /locus_tag="SCK08_002751"
     CDS             157..507
                     /locus_tag="SCK08_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1202576.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            921..1739
                     /locus_tag="SCK08_002752"
     CDS             921..1739
                     /locus_tag="SCK08_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1202577.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1812..2171)
                     /locus_tag="SCK08_002753"
     CDS             complement(1812..2171)
                     /locus_tag="SCK08_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1202578.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
     gene            2912..>3232
                     /locus_tag="SCK08_002754"
     CDS             2912..>3232
                     /locus_tag="SCK08_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1202579.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT         1114 a          472 c          568 g         1078 t
ORIGIN      
        1 cttcgcaacg atggcaaccg gagtttcttg atgaaaatat gcttctatca ttacaagctc
       61 gtttggtgta agatgggtat aagtcattta tgttcactct ccttgtatgc tttagcgggt
      121 attacaattt gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg
      181 gcgttttata aagcaatcat tcctgagaac ttagatgagg ttaaacttta tttatgggaa
      241 gataacggag aaattgtcgg ttttagtggt gtaagtgcag aagaattagt tatgctattt
      301 cttgatccag attttattgg taagcgatat ggtagtcaaa tattaattga gctgattgaa
      361 actgaagata taaaaagaat tgatgttaat agtcaaaata aatatgcaaa acatttttat
      421 ttaagccatg gatttgaaat tgaatctgaa gaagacttag atggttttgg taaaccatac
      481 cctataacgc atttagtaaa aaaataattc atgaatggtt tattacctga ttgaacatga
      541 ataaaagtaa tatttttatt ttgatggcct atttctaggt tatgaattat ccagatcaaa
      601 tttgtgtatt acattcagta ataaggtgct tatcttagga tcaacttatg atactagctg
      661 accaaggatg taccagttat agaaaatcag ttaaagtaaa aatgataaaa atcttatgtc
      721 aacactgatt taggctgacc tgtgggttag gagaaagaca ggtcggtagc ttttaataca
      781 aaaaatggct tgtatgtatc tagatagtta actgaaatta gaaatgttct tatttttaac
      841 ggagtaagaa aaataggctg ctttaggtct gaatactttt tcaatataat gaagatatca
      901 aaggcgacag gagtgttttt atgaataaga atcctcataa tctaaagttg gtagcagtag
      961 atatggatgg aacatttgct cgtactgatt acacatacga tatcccaagg ttcaaggctg
     1021 ttttatcgcg aatgaaagct gttgattgtc aatttgttgt tgcaagtgga aatcaatact
     1081 accaattgag atcgttattt ccaaattatt atgatgaatt aagttttgtt gctgaaaatg
     1141 gtgcttttgt taaagataaa acagaggtac tattttcagc cgatatgcct agaaaagcga
     1201 tcgaggtggt ccttgattta tgtaaagact atcctgaagt aaaaaatgtg atgtgtggtc
     1261 tgaatagtgc ctactgtgaa agaggaaagg tgagtcaagc attttttgat ctaaccaata
     1321 tctattacca tagattagaa tgggtagctg attttgcaga agttcaagat aaaattttaa
     1381 aatttgctcc gactatgcct gttgaaaaaa ccgaatttta cgttgaattg ttccgtgaaa
     1441 aattagccgg gctattagta cctaccagta gtggtcatgg ttctgtagac ttgattcttt
     1501 ctgagtgcca taaaactttc ggcttgaaaa tgttagttga gcgcttgggg attaatcctg
     1561 accagtgcgt tgcttttgga gatgggggca atgacattga gatgttggag tattgtggtc
     1621 tgagttatga aatggataat gcaacggaag ctgttaagca agttgctaag catcaatgtc
     1681 cttcaaatga cgaagacggt gttttagtga cgctcgatcg attgttccct aacacgtaaa
     1741 cactaggtaa ttcatcaaaa aacctcgata taagaaatat tcgaggtttt aaaactatat
     1801 ttagaacaga tttacgaagc gtctgatgag tcaaaataga cttggacatt gctatctaaa
     1861 ataccacttt cattctttaa acgaattgtt tgatttgatg gagttacctc taatactaaa
     1921 tgcccattaa taaaggtagt atcgtattca gatgttttaa ctgttgcttc ttcgcaaggt
     1981 attgttaaat catatgctgg aatataatgt tgtgtggaaa ctaataaaaa atgatcacca
     2041 atcatacagc atccctctgc tgaagaaaat tgattagagc catcatttaa agttaacaag
     2101 aatccatcct ttaaacgagg tacttgctta aatacttctg gttgtacttc taaaaataaa
     2161 ttatcgctca ttttcacgac caccatcgac ttccacagtt gaaattgaca ttttatttaa
     2221 cacacctatt cccccttaac tataactcaa ttctatataa aaaatattct attctcaaat
     2281 aattgaactt ttcaaatcgc caatttactt cggctttatc agagctcatt catatcaaga
     2341 gcgattaacg ctgtaaaaat taaaccgaag atctgataaa ttttctctat tttctacaat
     2401 atttaaaacg cttccttagt ctattatata tctatttctc ttaggagtat gtttagatgt
     2461 ttgaataata gttgcccaac tttaaaggtt attcttataa tattttactt cattttccaa
     2521 tagaaaattc aaaggattac tggtttttct aaaatagttt agtttaaata cataagtatc
     2581 ctccgacact gtattagttc ataatcatct aaatacaaaa ttttcaaata aaaatatttc
     2641 aagctctatc gttgacgaat gtaaacgata cttataaagt aatagaaatt gattacattt
     2701 gtaaacttaa aatggttaat ttatctcaca taaaactaag aataaaataa tgattaaaag
     2761 tggttagttt ttatacgatt tagcctatcg gtaaaaatga attagagagt gtaaaatatt
     2821 ttgtgtaaat gaaaaaatcc atacaaaaaa ggaagtcgct tctgtagaat aaagttaacg
     2881 acaaccaatt cacagaaaag aggacttccc tatgaatgat tttactacag aaattgtgca
     2941 aactctagtc actaaaggcg atttaaatga attattccgt tcgcacttag aaaaagcgat
     3001 aaacacactc ctacggactg aattaacggc ttttttagat tacgaaaaat atgatcgcac
     3061 tggttttaat tcaggtaatt cgagaaacgg ttcttacttt cgatcaatca aaaccgaata
     3121 tggtgaatta acattggaaa tacctagaga tcgtaatggt gagtttaaac aacaaacttt
     3181 accagcctac aaaagaacaa acgatacatt ggaaaccact attatccatt ta
//
LOCUS       DARBFD010000163         3221 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000163 DARBFD010000000
VERSION     DARBFD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.169"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            301..585
                     /locus_tag="SCK08_002755"
     CDS             301..585
                     /locus_tag="SCK08_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202580.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="SCK08_002756"
     CDS             588..1004
                     /locus_tag="SCK08_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202581.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="SCK08_002757"
     CDS             complement(1141..1860)
                     /locus_tag="SCK08_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1202582.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="SCK08_002758"
     CDS             2535..2630
                     /locus_tag="SCK08_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1202583.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="SCK08_002759"
     CDS             complement(2932..3177)
                     /locus_tag="SCK08_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202584.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DARBFD010000164         3179 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000164 DARBFD010000000
VERSION     DARBFD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.170"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(399..1013)
                     /locus_tag="SCK08_002760"
     CDS             complement(399..1013)
                     /locus_tag="SCK08_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1202585.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1619..1996)
                     /locus_tag="SCK08_002761"
     CDS             complement(1619..1996)
                     /locus_tag="SCK08_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202586.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2296..3177)
                     /gene="galU"
                     /locus_tag="SCK08_002762"
     CDS             complement(2296..3177)
                     /gene="galU"
                     /locus_tag="SCK08_002762"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1202587.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          968 a          656 c          491 g         1064 t
ORIGIN      
        1 gacaattttg ttatatcagg taaacttcat tgcaataatg ataacaaatg tactataatt
       61 actttgtaaa gagttttggg gaagactctt tcatcaacgt gtattaagag acaagaaaga
      121 atccatatgg attctttctt gtctcttttt ccactacttt ttatttttct gacactacat
      181 attcttataa ctcatttctc ataactgtga aacaacttat aaaaataaca aactttaaac
      241 aaaacaagct taaaatcaaa gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc
      301 gttgttaatc ctcattaata atcgttttct ttttttcaaa cgctccattt ttatgtggaa
      361 cattattttt ttaaggaact ttgtcaacat gtggataact attttccact tgtgtcctgg
      421 actagctgtt ttgctgtaaa atacaaaact tgctgcagca aaaagatcgt tgcaagagag
      481 gctgttccgc agatcgctgg atctagattt ggcaaagtca agactgcatc atgtaaattg
      541 gcgtggttgt ccaaaaccaa atcagcaaat tcaaataatc gcaaaccact gctatgttgc
      601 gatttgatac ttcgtgacaa atcaaatcca gtaacaatga ttaatggatg accattttct
      661 ttgatccact gagctagttc gatgattgcc gggtttcttc cttcattcga aagaagaaga
      721 aaaatgtctt ctgatttgac agaaagctga tcaataattg cctgtcctgc accttctagc
      781 gtttcataga cacctccggc tggattttca atcaatatac ttggtacaag atccggggtt
      841 tgatcccaaa attcaaatgc tacaccactt agtgtgcggc tagcatatat ctgcaatcta
      901 ccaccttgat taatttgttt cgcgatccaa gtgccagctt gaagcatcag cggctcttct
      961 atccttaatt tctcttccaa ttggtgataa tagtcaaaat aacataaact catttatctc
     1021 tcccgcctag ctatacttga catgaataga aacagctgcc taatctaagg aagtacataa
     1081 aaaaacagta gttagacagt aacacatgtg gtctttgctg tttcaaacac aaaaaaggca
     1141 ttgaacgaat acgcccaata ccttgacata gcaaataaaa caacgtgcag accatggaaa
     1201 atatctacat aggggtagaa ttttcaccct agattaggta caataaatta ggtcgatgca
     1261 ctgcattgac tatggcaaaa tgatcggtca gggtgtatgt gtgtgactgg tcattccctg
     1321 attcttagcg ccaactaagg gtcagggagc ctttttttat tcagctgttt ctatctgaat
     1381 ttattgtaag taatctctta tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg
     1441 atttatatat atataaataa cataaaaaaa ggaatggctt atcccgatac aacaagcatt
     1501 cctttaaaaa agattctttt atataagtga aaaaagcttt tttccaccaa ttaaataaat
     1561 ctctcatctc aatagtttat ccattatttt atcttttttt gcgtatacct actaaatatt
     1621 accgtcttct tctcgttctt ctactttgtt cgagagaatc tgtactagaa aaagtagttg
     1681 tatgtctacc ttttctgcct atccgagact ttcttttcat atatgggata taaatgatta
     1741 gatacagtac agcaaaaaat aaagcaaata cgcaagcttc ccaactacct aacgccaatt
     1801 caaccatcaa ggtagcaacc ccaacggatg ctgcatacca gataaaaatc agaaacgttt
     1861 ctttttttct gaccatgcga aacatgataa atagactaat aagcaatagt ggcaacacaa
     1921 atatccaatt gcttccttcc atcagccact tgagtatatc aagcagcgac tgtgcaacag
     1981 cgactaattt tcccatagtg aacacacacc tttttttata attaaaaaat tcgccttttt
     2041 attctactat gaatcttctg gcttggcttt catttctctt atgtaatcat tttcttttat
     2101 acaacttaaa aaaaaattaa accattagca taatattttc tgaatctatt cacgaaagtc
     2161 tttttctatc tttcgttttc cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca
     2221 gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa tagcctttca ttctcagcag
     2281 gccacttatt tccagttaac tttctgttct tagcttttta ctcaactgga taatatatgc
     2341 acttaatgaa gtcccaattt ccgaatgttg aagtccatat tcaatatttg tcttcatata
     2401 tcctaactta tcgcctacgt cgtatctttt tcctttaaac tctctcgcat agaccgcctc
     2461 tgtctggtta agagtctgaa gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc
     2521 ttgagtttct aatatctcaa atattttagg agtcagtaga taacgtccga taatggctaa
     2581 attacttggt gcttttccca tttcaggttt ttctacaaac ccttttactc tgcaacgacc
     2641 cttttcgcta tctgttttag gatcaataat tccgtattta gatgtttcat tttctggtac
     2701 acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa tccatcatca attgttgagt
     2761 caatgggata tcatctttca tcaaatcatc ccccagcatg acgacaaacg gttcatttcc
     2821 tataaatgct tttgcttgaa gcacggcatc ccccagtcct cttggatgag attgacggat
     2881 gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg agtaattctg ttttttcctt
     2941 ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag tgatcttcaa tgggtctttt
     3001 ttcttttccc gttacgatca agatgtcttc gattcccgaa tccaatgctt cttccacaat
     3061 aaactggatc gttggtttat ccacgattgg aagcatttct tttgccatgg cttttgttgc
     3121 cggcaaaaaa cgtgttccta gcccagcggc aggaatgact gcttttttga ccttcactt
//
LOCUS       DARBFD010000165         3083 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000165 DARBFD010000000
VERSION     DARBFD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.171"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..783
                     /locus_tag="SCK08_002763"
     CDS             <1..783
                     /locus_tag="SCK08_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HEG1202588.1"
                     /translation="YQKSYWGYSLPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFE
                     MMDEEKKVSYDKQYIEDLYYKYMETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEV
                     ERIKEFVTKEEVVIISVNFEYSHIDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASD
                     VYLRTSYKKLLSEREVVRDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSM
                     EIITKFALLDAMNVQMRDELSELAKKVKVTFLTDPRWVRIDK"
     gene            918..1496
                     /locus_tag="SCK08_002764"
     CDS             918..1496
                     /locus_tag="SCK08_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202589.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            1442..1960
                     /locus_tag="SCK08_002765"
     CDS             1442..1960
                     /locus_tag="SCK08_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202590.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            1968..3008
                     /locus_tag="SCK08_002766"
     CDS             1968..3008
                     /locus_tag="SCK08_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1202591.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
BASE COUNT         1101 a          330 c          629 g         1023 t
ORIGIN      
        1 tatcagaaga gttattgggg atattcatta ccaaactata tttcagcagt tcataatgct
       61 catcctaatt atgcaagcta tctgtcatat aagaatactt taacaattga agcaatgaat
      121 gaaatattcg aaatgatgga tgaagaaaaa aaggtatctt atgataaaca atatatagaa
      181 gatttatact acaaatatat ggaaactggg aaaatacagg aggaatatat ggaagaactg
      241 agaaaatttc tctcagacaa aaaaattcta ctgattgcac caggaaaaag ttctgtagat
      301 gaggtagaaa gaataaaaga gtttgttacc aaagaagaag tcgtaataat tagtgtaaat
      361 tttgaatatt ctcatataga tatagattac gtatttttaa gtaatttaag acgttttcat
      421 gaattaccga aagaaagcag aactaaatgt attgttacat caaatataca agcatcggat
      481 gtatatttac ggacgagtta taaaaagttg ttatctgaaa gggaagttgt aagagacaat
      541 gcaggattaa tggcaattag atttttagcc gatatgaatg tggatgaaat attcttagct
      601 ggatttgatg gatattcaca taatgaagat gagaattatg gtgagcaatc catggaaatt
      661 ataacaaaat ttgcattatt agatgcaatg aacgtgcaaa tgagagatga attgtcagaa
      721 ttagctaaaa aagtaaaggt gacattttta acggatccac gttgggttcg aattgataag
      781 tagtagtaat aatgttttta aaattttgat aaagagatag tgagagaata aaaatgggta
      841 aagtcttaaa taaaaaagaa gaaaaatatt taagggacga tggggcgaga tgaaactcta
      901 attttgaact tttaagaatc atttctatat tgtgtattgt tgcttaccat gctaatttgc
      961 gtttaaatta taatgcagac ggaattatag tacatggttc attaactgct aaccagattt
     1021 tcttgaatta tattggtatt tggggtcagc taggagttaa ttgttttata tttatttcgg
     1081 catatttttt aagtgagaaa agaaacattc actttgaaaa tgttttggga attgtattag
     1141 aatgtatttt ttatagtgta ttagtttatg gaattctttg tgcatgtgga atagaaacat
     1201 tttcattgaa cagctttgta aaagaaatat tatcatttgc tctaggtaga tattggttta
     1261 ttacatccta tttagcattg tatattctaa gtccgtattt aaatatcatg cttaacaatc
     1321 tggaaaaact tcagttgcaa aaaataagta tgttattgtt tataggtgta tgctgttatc
     1381 gtatgttatt taaaacatct ttgtttgggg attgtgctgt tttaattgca ctatatgtca
     1441 tatgttatac actgaaaaaa acgataataa tatttttgag agacacagaa tcataggaac
     1501 aattgtatgt ataacagtta taccacttct catatccata ggaatacttt tcaaatttat
     1561 accaaatgca atggttaacc agatggttca tattggttct ccattgatgg ttatagcaag
     1621 tatattttta ttttatgtat ttaaaaattt ttcattttac agtaagtata taaataaact
     1681 tgcgagaacc agtttagggg tatatatttt acatgaaaat cattatttca ttgatggtat
     1741 gaagtctaga ggcttaatat gggatgggat atttggatta ggcaaaaggt ttttagatag
     1801 ggatttttgg atttggttta taattgcagt tgtagttgtg tgcataattt gcatggctat
     1861 cgatgttgta agagaaaaat ttgaaagaca gatttttaaa attaagagtg taaagaacct
     1921 gtgtaaactt atgaatcatg ttataaatat atacccataa ttaataaatg aggagaaaag
     1981 aagtgaaagt tatatcattc ggagaattat tattgagact tgcagcaccc ggttattcaa
     2041 agttatttca aagagatagt ttggagacta cattctgcgg aggggaagct aatgtagcgg
     2101 tatccttagc aatatttgga tatcaatcag aatttataac aaagcttccg gattcagatg
     2161 ttggatatgc ggcagctaat gctttgcgtt attttggagt aaatgttgat aatattatat
     2221 ttggagaggg acgcatggga ctgtattatt tagaaaaagg agcagctcag agaccatcta
     2281 aagtaattta tgacagaaaa ttttctgcaa tttcgttagc tgatagtgat gaatttaagt
     2341 ggaaggagat cctaaacgat gcaaattggt ttcattggac agggataaat ccagcattgt
     2401 ctgagaatct ctttgagacg tccttagatg catgtcaggc tgccaaaaaa agaggtataa
     2461 caatttcttg tgatttaaat tatcgatcca agctttggac atcagaggaa gcaaaagaaa
     2521 agatggaaca gatactacca ttggttgata tttgtatttg taatgaagaa gatgcagaaa
     2581 aagtgctagg aattaaagct gagcatacaa atattaatag tggagaatta aataaagatg
     2641 gctatgtgca ggtggctagg gagattcaca agaagtttgg atgtaggtat gtagctacaa
     2701 ctctgcgtag aagttattcg gcaagtagaa atggttggag tgcaatgtta tacgaagcag
     2761 aaaaagataa agcgtatttt tccacagaat acaacataca aattgttgat cgcgttggtg
     2821 gtggagacag cttttctgct ggtataatat atgcgttatg tgaaaatatg tccggacaaa
     2881 aggctattga atttgcgact gcatctagtt gtttaaaaca tactatagaa ggtgatttta
     2941 atcgaactac aattgaggat gtatataatt taatgaatag cggtggaaat ggtcgcgtgt
     3001 tgcgttagtc aacatataaa gataaatcac agtaattttt atggtgatta tctgtcttag
     3061 tagtgattag aataacgtga aaa
//
LOCUS       DARBFD010000166         3054 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000166 DARBFD010000000
VERSION     DARBFD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.172"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..354)
                     /locus_tag="SCK08_002767"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="SCK08_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="SCK08_002768"
     CDS             complement(614..1162)
                     /locus_tag="SCK08_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202592.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="SCK08_002769"
     CDS             complement(1163..2020)
                     /locus_tag="SCK08_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1202593.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="SCK08_002770"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="SCK08_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="SCK08_002771"
     CDS             complement(2582..2911)
                     /locus_tag="SCK08_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1202594.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          893 a          620 c          384 g         1157 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac ctta
//
LOCUS       DARBFD010000167         3051 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000167 DARBFD010000000
VERSION     DARBFD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.173"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            202..1077
                     /locus_tag="SCK08_002772"
     CDS             202..1077
                     /locus_tag="SCK08_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202595.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="SCK08_002773"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="SCK08_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="SCK08_002774"
     CDS             complement(1642..2973)
                     /locus_tag="SCK08_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1202596.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         1080 a          553 c          467 g          951 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa t
//
LOCUS       DARBFD010000168         3024 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000168 DARBFD010000000
VERSION     DARBFD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.174"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1242
                     /locus_tag="SCK08_002775"
     CDS             <1..1242
                     /locus_tag="SCK08_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1202597.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1255..1887
                     /locus_tag="SCK08_002776"
     CDS             1255..1887
                     /locus_tag="SCK08_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1202598.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2092..2319
                     /locus_tag="SCK08_002777"
     CDS             2092..2319
                     /locus_tag="SCK08_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEG1202599.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2388..2654
                     /locus_tag="SCK08_002778"
     CDS             2388..2654
                     /locus_tag="SCK08_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1202600.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2644..2913
                     /locus_tag="SCK08_002779"
     CDS             2644..2913
                     /locus_tag="SCK08_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1202601.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1073 a          443 c          606 g          902 t
ORIGIN      
        1 aaaaccaaag atcagcttaa cgaatttttt gctaatgttc ttgaaacccc ttattggtat
       61 gcaaaaaaaa tcttaagcct tttatctgag tgtttagatc aacctgtgta tgaagaggac
      121 attttcttta ttacgctata tttttatagt ttgaatgtca cgaatgttaa aacaggtatt
      181 aagagtattg tgctagctca tggttattct acagctagta gtatggcgaa tgttgccaac
      241 cgtatgctga ataagaacgt ttttcaagct tatgatatgc ctttagatat tacattagag
      301 aaaattgaag caaaattgat tcaattcctt ggagattatc gaacagattc aggcttgatt
      361 ttattggtag atatgggttc actaaaccaa ctaggcggtt tattagtgaa tcatctaaat
      421 ggaccactgt taatcattga tcatatcaat acaccgttat tattggaagt cggccaacaa
      481 gtattaaaca ataaaagtat caccgaaata ggagcagaaa ttgaaacaag tcttactttc
      541 aaaaagcagt tactacttcc caagaaaaag aaaaagaaag ccatcattac ttgttgttat
      601 acagggattg gtagtgcgac ccagattcag gagattttaa cgaaatgctt gaaagatgca
      661 gttagagaat tagtaattat tccgtatgat tacaaaaagc tgttagaaaa caagaatttt
      721 gagactcctt ttcaattata cgatgtcatc atgattgtag gtacagaaga tcccaaaatt
      781 aatcagattc catatattgg attagatcgc ctgattaatg ggggaagcat tggcgaattt
      841 gttcattact tggaaaaaga gctgactatt gaagtgaacg atttacaaaa agaattaatt
      901 ttcaattttt caattagtaa aattgtcgaa aatttaacca ttttggatcc agataaggtg
      961 ctatcttttg tacaaaaagc aattaaagaa ttatcgaagt tattaaattt tgatttttct
     1021 aataatcagc tgtttttgtt gtacattcac tgtagttgta tgattgaacg aattgtacgt
     1081 aaagaaagtg tggatgagca agaggacatt gaagaatata tgcaagcaaa tggagaaata
     1141 atggataaaa ttcatagcgc tttttttgag attgaaaggg aatacacaat tgaaatacca
     1201 gatttagagt tgcgattatt gagcgatatt gttcaaggat aggagtgaat tcgaatgaca
     1261 atgggagtaa tttttgatat ggatggggta ctcatagata gcgagacatt ttattttgag
     1321 agaagaatga acttctttca agaactggag atagatccag gatcaaacca gattgatgat
     1381 tatgtgggga aaacagaagc tgcaatatgg gaaacattag ttcctgagga caaatcatta
     1441 agagaggaac tttatccgaa atatgtagat tatcgaaaaa cgcatccaat tgatttcgct
     1501 aaagctttac gaaaagaagt gaaaccgtta cttgacttcc tcaagaagcg ggatatacca
     1561 ttggcactgg catcttcttc tccagctgtc gagatagaga gtatgctcat tcaatgtgaa
     1621 cttactgaat atttttcctt tgttataagt ggtgagtcat taaaacaaag caaaccacat
     1681 cccgaaatat atttaaaagc gaaacagcaa ttagactgtg acttctttat tgcagtagag
     1741 gattcaacga ttggtattaa atcagcaaag gcagcaaata tctatacggc ggcactaaaa
     1801 caaccaatgc aaatcgatca gagtgaagca gacatacaaa ttgaaagtct acaagaactg
     1861 gaacaaattg ttttagaaaa aggttagtga attaatggaa ataaggctat atatctgtgc
     1921 tagctatttt gactaaccaa aaaaacttgc tataattcag gtgtagtaaa aggtgctggt
     1981 aacacctttt acgaaaatgc ttcggcagtg ataatgggat tggcttttta agttttttat
     2041 tttgttcgca atccctaagg tcatgtgaat gatcctaggg attgctagaa tattttgcaa
     2101 cagaaccaaa atacgaatag ttatcaagca gccatattga ctgcggtcaa tttaggaggg
     2161 gatactgata ctactgctgc cgtaactggt gctcttgcgg gtattcttta cgggggtgaa
     2221 gtgatccctg aaaattggag aaaatcgctt caaaatgacc agcttcttaa aacgatcagt
     2281 cagagatttt gcgataccgt gctatctcaa agaatttgaa taatggatat gatgcaaatc
     2341 ttgtttgtac taacgttgta ttgacaaaaa ggattttatt tagtacaatg ttagtacaaa
     2401 tgatgaacgg aggtagaatc atgtctcaat caaccattac atttaggatt cccgacgatg
     2461 aaaaagaact ggtattagaa tatgcaaaag cacacaattc ttcattgacc gaactttatc
     2521 gaaatgctgt actggataaa atcgaagatg aaatcgatct acggacttta cggagtgcaa
     2581 ttgaagtttc caaagagaaa aaagagagtg gtatttctca agaggaaatg gagaaactgc
     2641 taaatgaatt ataaagtcat ctacatgccg caagcccaga agcaattaaa aaaaatggac
     2701 agaaaccaag ctaaaataat tgtcgcctgg atcagaaaga atttagaaaa cacgactgac
     2761 ccgcgtcagc acggaaaagg tttgactgga aataagagtg gggaatggcg ctatcgaatt
     2821 ggaaactatc gaatattggc taatatttgt gattctgaaa ttgaaatcca gatattctca
     2881 attggacata gaagtacaat atataaaaga taaccgtttt tggaacgaag tagagtttcg
     2941 aaagtcaatg tataatcttt tgaattttgc ataataccac aggtagaaac tacccgatac
     3001 tatcggttgg tttctgcttt ttta
//
LOCUS       DARBFD010000169         3012 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000169 DARBFD010000000
VERSION     DARBFD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.175"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            177..359
                     /locus_tag="SCK08_002780"
     CDS             177..359
                     /locus_tag="SCK08_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG1202602.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="SCK08_002781"
     CDS             462..746
                     /locus_tag="SCK08_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202603.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="SCK08_002782"
     CDS             762..998
                     /locus_tag="SCK08_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202604.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="SCK08_002783"
     CDS             1020..1274
                     /locus_tag="SCK08_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202605.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="SCK08_002784"
     CDS             1356..1541
                     /locus_tag="SCK08_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEG1202606.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="SCK08_002785"
     CDS             1608..2003
                     /locus_tag="SCK08_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEG1202607.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="SCK08_002786"
     CDS             1993..2370
                     /locus_tag="SCK08_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202608.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="SCK08_002787"
     CDS             2392..2766
                     /locus_tag="SCK08_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202609.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DARBFD010000170         2940 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000170 DARBFD010000000
VERSION     DARBFD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.176"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            80..1768
                     /locus_tag="SCK08_002788"
     CDS             80..1768
                     /locus_tag="SCK08_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202610.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1755..>2940
                     /locus_tag="SCK08_002789"
     CDS             1755..>2940
                     /locus_tag="SCK08_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202611.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKI"
BASE COUNT         1004 a          429 c          474 g         1033 t
ORIGIN      
        1 aaatttaacg caaaacttat taaaaaaaga caattctagt atactgaaaa tagaattaaa
       61 aaaggagaac aacaaatcta tgaaaataag taaaaaagca tttcttttag tagttcttat
      121 cttacttagt actttatatt cagtaaattt tatgagaaat gctcaagaaa tttatacaac
      181 aggtgattta tcttttcatc tttcacgaat caaagggtta tcttctattt ttgaagggcc
      241 aatcaactat acaacattta acaattatgg agatggactg aactattttt atccttttct
      301 tacaataatt cctgcagtcg ttttttatgg aatttcaaat aacttaatat taagttatgt
      361 tctatatatt tggttattaa atatttgtac gattctgatt agtttttatt atggagaaaa
      421 gttttttaaa aagatagatg ctgctttttt atttagttgt ttatatactt ttgcaactta
      481 tcgaacagtt gatatctatt atcgatccgc aattgcagaa gcaatcgctc ttacgattat
      541 cccagctgtt ttatattatg cttatggatt gatattcaat agagaaaaaa aagtcatcgc
      601 tttatctgtc agtataagtc tacttgtgta tactcatgtc ttaactacac tattgtgtag
      661 tgcattgatt gcttgtatga ttatagttcg tttgttttta agcaagcaaa taaaaaacga
      721 tataaaattt gtggtcgtga atttagtaaa ggctgttttt ttgacttgtg cattaactag
      781 ctatttttgg tatcctatgt tgcagcagat gaattatcaa gaaatacaca gtcccttcaa
      841 aacagatttg cagctagaag cactttctat ttcagatagt atcattcaag cgttaaataa
      901 cgatattaca acatacacta tggggttatt aggattggtt gcgttgattt ttcctttatt
      961 catactgaaa gaaatggaga aaaaagaaaa aatcatctat gttggcgtag tttctacatg
     1021 ggtatgcgta actaatttat ttccatggtt tttgtttcaa aaaacaccga taagcttact
     1081 ccagtttcct tggcgtatct taggtatcca aatctttttt tcttcaatga tcataacgct
     1141 tgtttttatg aaaaagacta tgaataaaaa atattcatgg ttaatcattg gcgctactgt
     1201 ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat tacgcaaaag ttatccaatc
     1261 aaaacacggt catattgtta tcaatgaaga aacactccca cgttatacaa catcagatat
     1321 gggaggatta tacgattacg ccccaacaga agcgataaaa gaaaggcctc acttagaaaa
     1381 acatgaagta aaagtaggag aatcgtggga aaaagggaag tataaagctg cagataataa
     1441 cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg acagtaccag tatatgctta
     1501 tttaggtaca caggtaaaag taaacggtaa agtcgtaaat gactcttata aaaaacatgg
     1561 cttagtgaat gtggatgtaa atgctggaga aaataagata gaaattacaa caaaatatac
     1621 tccaactgct ctcatggctt tatggatcag tattctgact tattgtttag tggtgtattc
     1681 aagttttaaa cgatttgcac ctaataagtt gaaagattct cgatatgtga ctaaaaagga
     1741 gataaacaag aagaatgaaa ataaataaaa aagtagtaat attgagtgga ttaattatag
     1801 ttagtagtat atattctttc atctttttca aaaatacaca gagtatttat accactggag
     1861 atctttcttt tcacttatct cgaatcaagg ggttatcgac tatttttacc agtccgatta
     1921 attatgagac attcaattat actggatatg gtgtaaatta tttttatccc tttctgactt
     1981 tttttccagc tgtaatgtta tattggatga caaaaaattt gattgtaagc tatattattt
     2041 acgtttggtt attgaacttg tgtacaacac tcgtagctta tcattatggc gagcggtttt
     2101 taaaacaaaa aaaagcggca tttttattta gttgtctcta tattttctct gcttatcgga
     2161 cagtagacat ctactatcgt tccgcaattg ccgaagctat cgcaataaca cttgttatcc
     2221 ctgtattatt ttatgcttat caaatcatct ctggaaaaga agaaaagtat ccgtctgtaa
     2281 agctagcatt gagtatgagt ttgctagtgt acagccatgt actatcaaca ttaatgagta
     2341 cagcattaat tatcattttt atttttattc gtttggtttc taaaggtttc aagaatgcag
     2401 attttattgc tatatttaaa aaattattta gtgcggcagg aatgacattg gttctgacga
     2461 gtgctttttg gtatccgatg tttgaacaaa tgctctacca aaaaatcaac aaacctagcg
     2521 tgacaaattt atatgctcat gcatctaatg tgttcgatag tctgactgaa gctatgaaca
     2581 acgacttaac tacttactca atgggactag ttggtctgtt atctttatgt attcccctga
     2641 ttttatttaa aaaattaaca aacatagaaa aaaagattta ttatggtacg tgtttgactt
     2701 ggctagctac aactagtttg gtgccatggt atcttctgca aaatacgcca gccaaattgt
     2761 tacaatttcc ttggcggatc ttaagcttgc aaataatatt ctcttctctg atattaacga
     2821 tgatattttt caaaaataga agatacaata agacaagaga actattttat ttatttatga
     2881 ctgttttgtt attaatcact ttaactgttt ctagtaaaaa aaatttcaat caaaaaataa
//
LOCUS       DARBFD010000171         2939 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000171 DARBFD010000000
VERSION     DARBFD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.177"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(61..1371)
                     /locus_tag="SCK08_002790"
     CDS             complement(61..1371)
                     /locus_tag="SCK08_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1202612.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="SCK08_002791"
     CDS             complement(1383..2801)
                     /locus_tag="SCK08_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1202613.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          927 a          608 c          455 g          949 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatg
//
LOCUS       DARBFD010000172         2914 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000172 DARBFD010000000
VERSION     DARBFD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.178"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(45..1364)
                     /locus_tag="SCK08_002792"
     CDS             complement(45..1364)
                     /locus_tag="SCK08_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1202614.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="SCK08_002793"
     CDS             complement(1361..2014)
                     /locus_tag="SCK08_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1202615.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..>2914
                     /locus_tag="SCK08_002794"
     CDS             2302..>2914
                     /locus_tag="SCK08_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1202616.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          955 a          474 c          321 g         1164 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgaca ccttctgttg
     2101 ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc tttagaataa
     2161 agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tattgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc
     2701 tcggatggga cggtgacacg gatcttgcgt gaagcaacga agcattacca gccacgtatg
     2761 aacttccttc cgacggtgtt gtgtatggat gagttcaaat caatgaagtc tgtttctggt
     2821 tccatgagtt ttatttgtgt tgatgggaca acccatcagc tatttacgat ccttgaagat
     2881 cgcagacttt acaaactaac tcaatacttc ctgc
//
LOCUS       DARBFD010000173         2907 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000173 DARBFD010000000
VERSION     DARBFD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.179"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1412
                     /locus_tag="SCK08_002795"
     CDS             <1..1412
                     /locus_tag="SCK08_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1202617.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="SCK08_002796"
     CDS             1546..2889
                     /locus_tag="SCK08_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1202618.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1143 a          398 c          567 g          799 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatc
//
LOCUS       DARBFD010000174         2827 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000174 DARBFD010000000
VERSION     DARBFD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.180"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            799..1053
                     /locus_tag="SCK08_002797"
                     /pseudo
     CDS             799..1053
                     /locus_tag="SCK08_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1123..1635
                     /locus_tag="SCK08_002798"
     CDS             1123..1635
                     /locus_tag="SCK08_002798"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1202619.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1632..2669
                     /locus_tag="SCK08_002799"
     CDS             1632..2669
                     /locus_tag="SCK08_002799"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1202620.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1023 a          496 c          564 g          744 t
ORIGIN      
        1 attgatgacc acgaaagtgc atggcgatat ttgatgttga tactttatgc agtaccctat
       61 gtattcttcg ctatcattct gtggctcata gcattgtttt ttagagtatt taatgacctt
      121 taggtagatt tatatataaa aagggctaaa tacgcaaaat acccccttaa aatgcaaaat
      181 aaatgcaaat aagggggtaa ctccttatga aaaaacaact aatagtatta taacacgttt
      241 ttatgccgag aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt
      301 tggttggact tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc
      361 ctgtaggttc cgaccaataa ggaaattgga ataaagcaac gaaaggagtt gagtaaaatg
      421 agtgagaaaa cgaagaaaat cattcgagta tcattagctt tttcacaagt agtagtgcaa
      481 tcattgatag tttattatac ttaccagcaa tataaattat ctaaagagaa ttaagttttg
      541 attatgtaat aagttttggt actaaaaaag acttgatcta attagaccaa gtcttttgtt
      601 agtgttatat taataacaaa ataaaaataa gaagtcgctc actccccgac caaagtttgt
      661 gagcaaaata aacagtcgat gtttgtttct taattgtata tcttgatatt aaacgattta
      721 agattattct tcaagatata tattcgcaaa tgtaattgga aaagcgactt cttaaacaaa
      781 attaaggagt cgctttttat ggcaaaaata acgaaaaatg agcaagaaaa tttggtggaa
      841 aattacacaa aaaaaccggc gtactctaat agccggttag acgcacattt cgtgtgcact
      901 tctaatccga aattgagctt tgacgctatg acgatagtcg gaaaccttaa taaaaacagc
      961 gctaaaaagc tatccgattt tatgagttta gatccacaaa ttagactttg ggatatactt
     1021 caaacaaaat ttaaagctaa agcactacaa gaccccctat gttaggctag ttgtcgtata
     1081 ggataataag ttataataaa gactatacgg aggatgatta ccatgacaaa taaaaacaat
     1141 gaacgttact ctaccgaact gaaagcgaca gccatcaaga aaatgatgcc acccgaaaat
     1201 agggggattc gccaattaag tcaaactttg ggcattccag aagcgactct ttattcttgg
     1261 cgtaaaaaag ctagaattca agggaatact acacccggaa cgaaaaaaaa tgtcgaaaaa
     1321 tggtcaagtg aagataaatt tttaattgtg atggaatcat tctcaatgaa ccaagtagaa
     1381 cttgcggaat attgccgaaa gaagggctta tttaaagaac aaattgaggc ttggaagaaa
     1441 acgtgcttat ctgcgaatga acaggaagaa aatcgaacga gagaacttgc aactgaacta
     1501 aaagaagaga aaaaacaagc gcgtcaatta gagaaagaat tacgtcataa ggaaaaagca
     1561 ttagcggaag ccgctgctct cttattacta agaaaaaaag cacacgcgat ttggggggac
     1621 caagaggaag aatgatttca tcttcagatc gcaaacaagc tgttgaactt attcaagaag
     1681 ccaatcgcaa tggggcccgt ctcacctatg cctgtaatga gctgaatatt agcgttcgaa
     1741 cctacgaacg atggacgcgt gaaggtacga tagcgcatga tcaacgccct ttggcgaaac
     1801 gtcctgtacc caaaaataaa ctgacggatc aagaacgcga aaaaataata gaaaccgttt
     1861 caaaaaaaga atttatgaat ttgccaccta gccaaattgt accaaaactt gcggattgct
     1921 cgatttatat tgcatcagag tctagcttct atcgtgtatt gcgagaaaaa aatatgcaac
     1981 atcaccgtgg ccgaagtcaa gtttcacaaa aacgcatacc gccaagtcac cttgcgacga
     2041 agccaaatga agtttggact tgggatatta cttggctaaa aggtccaata aaaggactct
     2101 tttaccggtt atatctcatc attgatttat ttagccgtaa aatggtaggc tgggagattt
     2161 gggaatcaga agaagctcgt catgccgaaa aacttattaa acagacggtg cttaaggaac
     2221 gtatccaagg acagccgttg gttttacact ccgataatgg cagccctatg aaagccgcga
     2281 cttttcaagt cttactcgaa aaattaggaa ttcaaagttc ttattcaaga ccacgagtga
     2341 gtaatgacaa tccttattcg gaagcggctt tccgaacgtt aaaatatcga ccagaatatc
     2401 ccaataacgg ctttaaaaca atagatgatg ctcgaaaatg gacgaaagag tttgtcgatt
     2461 ggtataacga ttctcatttg catagtggga ttcaatttgt gtctcctgca gcgtgtcatg
     2521 aaggacgaca tctcgctatt ttagagaaac gaaagcaggt ttacgaggac gctaaaaagc
     2581 gccatcctga acgctggtca aaaggtgttc gaaattggac tccacatcaa tcggtggcgt
     2641 tgaatccttt aaaagaaaca gaaagttaac aggagcataa tctttcaaga ttatcaaata
     2701 accctcgtca cccaataggg gaaaaagtta ttcttcccct attggataac gaggccaacc
     2761 gaccataggg aggttctgct ttttgatagg tggaaaataa aaatacgaca actatattga
     2821 caaacac
//
LOCUS       DARBFD010000175         2759 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000175 DARBFD010000000
VERSION     DARBFD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.181"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            5..190
                     /locus_tag="SCK08_002800"
     CDS             5..190
                     /locus_tag="SCK08_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1202621.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            202..450
                     /locus_tag="SCK08_002801"
     CDS             202..450
                     /locus_tag="SCK08_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202622.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            481..660
                     /locus_tag="SCK08_002802"
     CDS             481..660
                     /locus_tag="SCK08_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202623.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(703..1173)
                     /locus_tag="SCK08_002803"
     CDS             complement(703..1173)
                     /locus_tag="SCK08_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202624.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1260..1958
                     /locus_tag="SCK08_002804"
     CDS             1260..1958
                     /locus_tag="SCK08_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG1202625.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2136..2477
                     /locus_tag="SCK08_002805"
     CDS             2136..2477
                     /locus_tag="SCK08_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202626.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2470..>2759
                     /locus_tag="SCK08_002806"
     CDS             2470..>2759
                     /locus_tag="SCK08_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1202627.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         1016 a          400 c          558 g          785 t
ORIGIN      
        1 ttttatgtct gagaatttag acttaaaaat tcgagcggag atgagaaaaa gaagaatgac
       61 tttcaaagaa ctagctgcgc ttatcggtat ttcaggagct tatttatcag atattctaaa
      121 cggcaatcgt gatggaaaga aagcacaaca gcatatcgaa acagtgaaaa aaatattgga
      181 catccgatag gggggtaggc gatgacaaaa ctaaaaaaac aagattttgt aaaaaaatac
      241 aattattctc catctactta tcaacgtcga atgtcggaac taaaaaatac agcaattttc
      301 tcagcggcgt atgaacgggt cacaggacaa gaagtttgga tcaatacaga attatacgat
      361 aaatttttgt ctttcaaatc ctataacagg ttacgcacaa gaaaggtaac acctaaagaa
      421 tttatcgaga agcatttagt tgatttataa aaaacagaac attttgagag gtgaaggtta
      481 atgggtaaat tcaacagagc attggtattc agcgcaccgc taatcatcta cgctttagga
      541 ctttggggaa gcaggcaagc gttgatagga acgattgttt acatggtttg gatttttatg
      601 gggcttgatg aagctgaagc tgagtacaga gcgaaaaagc caaccgaggg ggctgactaa
      661 aaatgaatag aaaagagaaa ctagaatgta tattactatt actcagttta attctttcac
      721 taatttctct attgggaagt ttgtattttt gatatcaaca attttgtgga tttgggttct
      781 atttagagta aatatcattt ttgtttggtg attaaggagt ttattaaaat taattttccc
      841 tacttcaaat gcaaggtaac aagatatagc acttttagga ggaatattta gcggtacagc
      901 ttcagagtat attctattat catcgactga tcctccttta gcaattaatt tcttaaatgg
      961 agtagctgac catctgcggt ttaagttagc ggatgaaagt tctaaattaa ctaacatagc
     1021 tggttctgtt gaatagttag aaatgattac ttttgtataa atcatatctg cagcgaagta
     1081 agaagcatta agttcaactt ctagttgagg gcgcattcct tttattttta tccctgttaa
     1141 aaacgtactc aatataaaac ttattattga catccattga aaaattgtta agtgaaaaaa
     1201 attcaaaata aacaccacca gtttttaact aaattatacc aaaaaggaga gaagaaataa
     1261 tgcaagaatt agtaattttg aaaaataaag aagctgtgac tacgagctta caagtggcag
     1321 aaagcttcga gaaaaaacat caacatgttt taagagatat tgatgcacta aaaaaagatg
     1381 tgtccaattt tggacagatg tttgtagaag gtaatgaacc agattcatat ggcagaaatc
     1441 gacgagtttt cttcattagt agagatggat ttttcttgct ggctatgggg tttacaggaa
     1501 agaaagctat ctacttcaaa caaaaataca ttgaagcatt caacgaaatg gaagatgtta
     1561 ttcgcaagaa tactgttcct caaacaattg aagatatgat gatctatcaa ttagaagaaa
     1621 tgaaagatgt taaaaaagat gtttccatgc ttaaagatac tatgcgaatt agcggacaac
     1681 aagagtttga aattaagcaa aaaggaaata tgaaagttat gggagttcta gggggaaaag
     1741 aaagccgagc ttatgaagaa atcagcaaaa aagtattctc aaaattttgg tctgaattta
     1801 aacgtacctt ttcaatccca agatatggcg agttacctcg taagagattc gatgatgctg
     1861 tttcatttat tgaaatgtgg ttgccagaaa ctgcgatccg tatggaaatt gatcaactga
     1921 acagacaaca gagacttttt ggtgatgaca atgaatagag ctgaagcgct aagaataggg
     1981 acggcaattg ctaattgctg gtggaaatac tataaaccaa tcatcctaag ccaacaacat
     2041 attgacaagc aaaaagcatg gcaacaaata aaaaagtgac tccgccggca agcaaagagt
     2101 cacaaagaaa acacatcata aggagatttt agcatatgga aaaagaactt tccactctag
     2161 atcaatattt gattgatcct gattggggca agccgaaaat tgaggaaaca agtggtcgaa
     2221 aaatcagacg aaatcttttg acgaatgaag aactagcttg ggatcaagat gatttaggca
     2281 accatgtaac tatttgggat catgtttatc ttatccatct atcgaagcat tcgaataaac
     2341 ctgaatatat ttacgtcatc gaagatggct tgattgatgc gctagaagag tacgacagag
     2401 ataacttgat tgatatctct tattacggac caggtaagaa atacattgct gaaatggagg
     2461 cagaatttga tgagtgaaat caaagggaca acgaactttg aaaaactttt tagtcgtaag
     2521 ttaaataaaa ttctcaagaa aaaaggaaat tttgattatt tatcttgggc tcacgcgtgg
     2581 gagattatga aaaagaatga tccacaggca acggtaacta ttaatgagta caaacactac
     2641 agggttgttt ctggaactca tcaagacttt cttgttgagg aatataaacc ttttcttatg
     2701 gatgaaactg ggacttatgt atctgtctca gtaacggtta aaggacacac ggaaaccga
//
LOCUS       DARBFD010000176         2745 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000176 DARBFD010000000
VERSION     DARBFD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.182"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            419..>2745
                     /gene="esp"
                     /locus_tag="SCK08_002807"
     CDS             419..>2745
                     /gene="esp"
                     /locus_tag="SCK08_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEG1202628.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          994 a          422 c          543 g          786 t
ORIGIN      
        1 ataaagaagg tgatttcaaa ttccgaagaa ggaatttgaa gtcacctttt tacttatgtc
       61 acagtctcat tcagtaatgc gggataaggc tccttattcc gttatttgaa tactctgaag
      121 tttaatctaa gaaagggtaa tattttgaaa acatgcttca tcgcatagaa atgaacttaa
      181 tttcagaaag aatacataca tatcttttta tatgttttca gctgctgaat ggttaaaaag
      241 cacaaatatc cgaaaaaaac atgaagaaat gtactttttt tgttgtaatg ttcatgaaat
      301 tctctaagta aattagttaa cataaggtta gtaagattcc tattctgtga ataatacgta
      361 tcagatttat cttttattct tactaggaaa gtaattatta agggagttga tttgaataat
      421 ggttagcaag aataataaga gagtatttct agaaaaaaca aagaaacgag tattgaagta
      481 cagtatcaaa aaattaagtg ttggggttgc atctgttttg attggtgttg gtcttgtctt
      541 gggaacaact gaactagtta aagcacaaga tgaaatttct ccaagcagcc cacttgaaac
      601 agcgataagc tccgtacaag taggtgacaa agtagcttcg gggaatactt ttcaggagaa
      661 tcctggttat acgaaaaatt ataacttttc ggatttacaa tttagtcctc aagaactaac
      721 tggagacaca ctaaaaggca atacaattgg atttgaagtt tacggaaaac ataatattgc
      781 agcatcaact aaaaactggg aaatccgtct tcaattagat gaacgattag ctaaatatgt
      841 agaaaaaatt caggtcgatc caaagaaagg aattggatct tctagacgga cctttgtaag
      901 aattaatgat tcgctgggta gacctacaaa tatttggaag gtcaattata ttcgtgcaag
      961 tgatggatta tttgctgggg cagaaacaac cgatacgcaa accgctccta atggggtaat
     1021 tacgtttgaa aagagcctag atgaaatttt taaagagata ggcatagaca atctcaagac
     1081 cgatcgttta atgtatcgaa tctacttggt aagtcatcaa gacgatgata aaattgtacc
     1141 gggtattgat agtacgggct actttttaac agattcagat gatttctata atagcttaga
     1201 tgtatctgaa aataatcctg accagtttaa acatggatca gtaagtgcaa aatatgagaa
     1261 acctaatact caaacaaaag atggttcagg atccacaggt gctaatggag ctataatctt
     1321 agatcataag ttaacgaaaa attataattt ctcatattca tcttccgcaa agggcacgcc
     1381 ttggtatgct aattacaaaa ttgatgaacg attagttcca tatgtagcgg gtatacagat
     1441 gcacatggtt caggctgaca aagtaacata tgatgtttcg tttgaatccg gaacaaaagt
     1501 agctgattta gcaatcgagc gacgggaagg tcatgaaaat tatggcatag gttcaataac
     1561 taataatgat ttaactaaac ttatcgattt tgctaatgca agtccacgtc cagtcgttat
     1621 tcgatatgtt ttacaattga ctaagccatt agatgaaatc ttagaagata tgaaagcaac
     1681 agcacaagtt gaagaaaata aaccatttgg tgaagatttc atctttgatt cttggttgtc
     1741 ggatacgaat aaaaaattaa tccagaacac ttatggaaca ggttattatt atttgcaaga
     1801 tattgatggt gatggaaacc ctgacgataa agaagagagc ggagacacga atccatatat
     1861 cgggaaacct gaattagaag aagtatatga tgttgacaca acagttaagg ggaaagtatt
     1921 catccacgag ttagcgggaa caggtcacaa agcccaactt gttgataaag aaggtactgt
     1981 attagcagaa aaaactatcg ctccaaatga aaaagatggg gctccaattt cagatactgt
     2041 agaatttgaa tttacgggtg tagattcaag taaattaatc gcgaaagatg aattaaaaat
     2101 ccaaatcgtt tctccaggtt ttgataaacc agaagaaggt tcaaccgtta ttaaggaatc
     2161 accaaaagcg gttgataaac aaaccgtggt agttggattt aaaccagatg ctaaagaatc
     2221 aattcggaat aataaaaact tacctgaaga tgcagagtat tcatggaaaa cagagcctga
     2281 tacttctaac gttactgata gtacgaaagg tattgtaact gttaagatcg gaaatcgaac
     2341 tttcgacgtg gatgtagagt ttgctgtaaa agcttctcaa gctatggaaa atgatgcaac
     2401 atacgtacct ataacaacaa ctccagaaac gacagttcaa agtggaaaac ctacatttga
     2461 taaaccagat gttcctttag ctaaagatgc cttttcaatt tcagatgtct ataataagta
     2521 cttcggtaat gcaagtgttg atgcaaacac tggtattgtt acattcactc cagctaaagg
     2581 tgtaggagaa tcggagccga ttactggaac aattcctatt aaaattgttt accaagatgg
     2641 ttctgtaggc acgaccgatt tagcagtaac tgtaagtaaa gatatttatg aaaatccagg
     2701 agaaaacatt cctgcaggct accacaaagt aaccttcacc gcagg
//
LOCUS       DARBFD010000177         2654 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000177 DARBFD010000000
VERSION     DARBFD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.183"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(261..545)
                     /locus_tag="SCK08_002808"
     CDS             complement(261..545)
                     /locus_tag="SCK08_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202629.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
     gene            complement(566..1936)
                     /locus_tag="SCK08_002809"
     CDS             complement(566..1936)
                     /locus_tag="SCK08_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HEG1202630.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            2155..>2654
                     /locus_tag="SCK08_002810"
     CDS             2155..>2654
                     /locus_tag="SCK08_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG1202631.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSQLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVT"
BASE COUNT          824 a          539 c          394 g          897 t
ORIGIN      
        1 tatgtgaaaa tcgaatcttg agatagctat tccccttgag atattcactg atacttctac
       61 tcaaatcatt taaattttta taattatttg tacggaacgt atatatatca acatctattt
      121 agacgttgtc ggtgatacaa tgtcattttt cccgtgtact atgtcattta tctctatact
      181 ttgtcatttt tctttttggg caatcaaaaa aggcaacaaa ccaattatga tttgctacct
      241 tctccgttta aaaacttatc ttagccccaa aatggaatcc aatttttaac ttcttttgcc
      301 aatgcatacg ccttagcaag acctgagttt tcaactaaat atttttcccc ttccagagaa
      361 acactccccc agatatgaac agtgttatcc gcccaaacca tggattctag taagccattg
      421 cgcgaaagaa atttagtttg ttctaaaata tcttcttctg gaacttgccc tttaagctca
      481 ataaaaggat tctttttttc atcacgcaat ttaagaattg catatcttag tttgttctta
      541 tccaacctaa caccacctct atgctctatt tcttatttct tagcgaatca ttaatactta
      601 ttccgttatc atatttccag tagtcccaac cattccggct accccctaat agcagagctg
      661 ctgcattcga aacagatgag gcagtgtaat cttgaacaaa ttgtagtccc ccaattgttt
      721 cttcaatgat tccattatcc aacagctctt ggcgagtttc atctagcgaa tcagatgcaa
      781 tatctttaat cttagaataa atttctgaac ctgaatgtat aatcacggtc tcgtcttcgt
      841 tcaaataccc agttgctttt attccttgcg cctctaagaa aatttcccta ttcccatcgt
      901 gttcatagtc gcccgacgat ctcaaatagg gatacatagt aatcagttca ttgattagag
      961 cttcacttct ggcagaaata tccgatgatg tccactcagt caattcgtac aaatctttat
     1021 tcatacgaat atgaagggta tcttcaaaga ttttcttttt ggtctcaaag tctttatttc
     1081 ccgcggccga attatcagga tttgtaacga gtgttaaatt accaatggta tttactaatc
     1141 ctgtgtattc ttcccccgat gtaccagcct tctcttccca atatgaagta ctagtctgtg
     1201 gcatgatgtg ttcaatggac aagttactca tatcaagtgt tgctgaattt tctcgattct
     1261 ctattttttc aagtaaccaa cgggccaatc tcatcgcata agcattggca ttcaatagat
     1321 tagatttcaa tgccttgtca gatggaagtg ccatattatt tgattgattt ctagtaacaa
     1381 gaacataaat tacaatatca acgatatttt catatccatt agctttagcg tacttcctaa
     1441 cacttttcaa atatgaagga aatgcttttg acactctgct tgtatcatcc ccattaaaat
     1501 atctcctaag ctgataatca ttcactacct taatcgcatc aatatattgg tcttcagtga
     1561 taaattggtc atgatagtac cattcagata atcccaaaac aaaaggtgca ggcatcatac
     1621 tttccatact ttggaaatcg ctcaatactt ctgagtattt tccttcaggt ttgttatagt
     1681 aaagtctcgc aaaataacca gcatatcgca ctaatttttg gagcaattcg gcatcacttg
     1741 atacatcgcg ctctttatgc caatagtctt tgaatgcttg ataaagaata tgctttgctg
     1801 aatactcccc agttattgca gcaaggtagt atctgaaaaa gtcttccatt cctttagaat
     1861 tatcaaagac ttcttccaac ctacgccaat atttagtata aagcgtagtc tgctcttcat
     1921 tagatttatc catcatgatg aagtttcgaa tcaaatccga ggcagtcaat ctttcaccgg
     1981 tagaattgat actttcaaaa atttgctgtg cattatcacg gtcatctaat ttaatataaa
     2041 caatacgcgt ggtgctagtt aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa
     2101 tcaacaaaaa acgcatatag tagtctcaaa actcccaaga aatgaggact acatatgcgt
     2161 tcatcaaaat atactgaaca ttatacagat acttttataa cattcaagga aattatgaga
     2221 actgtttcta acatatacca caattgtgta ccagataaga ttaaaaaccg aagaaatact
     2281 gatcaactga agcaacgcga tacagtgatt attgcttgtg ttatatgggg cataattaat
     2341 ggctatacta gccaaagagc cacgtataga gcggtttgtt ccgtcttatt tcttaatggt
     2401 gactttccta gtaggagtcg attcactcgc ttaagttcaa acttagctta cactatcaag
     2461 attattcgat actttttcat caagaagctc accaaaggtg aactagtcgg aattatagat
     2521 agttttccta gtcaattatg taaaccagtt agaaatagac aagcaaaact attgaatcaa
     2581 atagccaaag ttggctataa ctcaacaaaa aaatcttatt tttatggtct taaaattcat
     2641 atgatcgtta ctaa
//
LOCUS       DARBFD010000178         2640 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000178 DARBFD010000000
VERSION     DARBFD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.184"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..153)
                     /locus_tag="SCK08_002811"
     CDS             complement(<1..153)
                     /locus_tag="SCK08_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1202632.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="SCK08_002812"
     CDS             complement(475..696)
                     /locus_tag="SCK08_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202633.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="SCK08_002813"
     CDS             complement(794..1039)
                     /locus_tag="SCK08_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202634.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="SCK08_002814"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="SCK08_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2263..>2640)
                     /locus_tag="SCK08_002815"
     CDS             complement(2263..>2640)
                     /locus_tag="SCK08_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1202635.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          889 a          465 c          362 g          924 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
     2341 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
     2401 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
     2461 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
     2521 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
     2581 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
//
LOCUS       DARBFD010000179         2557 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000179 DARBFD010000000
VERSION     DARBFD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.185"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..498
                     /locus_tag="SCK08_002816"
     CDS             <1..498
                     /locus_tag="SCK08_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEG1202636.1"
                     /translation="IEKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTD
                     IIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIIN
                     ANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQ
                     KQIAY"
     gene            471..1148
                     /locus_tag="SCK08_002817"
     CDS             471..1148
                     /locus_tag="SCK08_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG1202637.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1138..2280
                     /locus_tag="SCK08_002818"
     CDS             1138..2280
                     /locus_tag="SCK08_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEG1202638.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2293..>2557
                     /locus_tag="SCK08_002819"
     CDS             2293..>2557
                     /locus_tag="SCK08_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1202639.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNI"
BASE COUNT          951 a          334 c          501 g          771 t
ORIGIN      
        1 atagaaaaac aaattttagc tttgatcaat tcaatggaac aacaatctca accaattatt
       61 tgcattttac ctaatagtga tgctggtagc gaaattatca gaaaaatgta ccagaaatat
      121 ttaaaaacgg atattattta tattacgaat ttagagccgg aggaatacct gactatatta
      181 aaagagtgca aatttatgat cggaaattct tcttctggga taattgaagc tgcatcattc
      241 aaaataccag taataaattt aggaaccaga caaaatggac gagaacaatc taaaaatatt
      301 ataaatgcaa atctaaatga aaatgaaatt gaaactgctt tgaataaaat acaagatcct
      361 cagtttataa aaagcttgaa aaatgttaag aacatctacg gtgacggcaa cagttctaga
      421 agaataacaa aaacattgaa tcaaatattg aatgatgggg aagatattaa gtggattcaa
      481 aaacaaatag cttattaaaa tatactagtg atccatacat tattattggg gctggcggac
      541 acgcaaaagt cattattgaa accttaagat tagaaaataa gttaatatat ggattttttg
      601 atgataagca aaataaaatg caagatctga aatatcttgg gaaaataaac gaattgaata
      661 gctgggcgga tagcaaaaaa aaaatctata aatttattgt agctattggt gactgtgtag
      721 taagaaaaaa gattataaaa aaattgaata tgccaagtga gcaatatgga atagctgttc
      781 atccaagtgc tatagtggca aaagaaattt caattggtta tggaactgtt atttttgcga
      841 atgctgtaat taacccagat gctgtaattg gagagcatgc tataataaac acaggtagca
      901 ttgttgagca tgattgtaga attaataact atgttcattt atctccaggt gtatgcttag
      961 cgggtggagt acatgtaggt gtaggaactc aaataggaat tggttctcaa tgtatccaga
     1021 tgaagaaaat tggatcttgg tgtatgatag gcgcagggag cactattgtt aaggatattc
     1081 cctcacattc cttagcttat ggcaatccag ctaaaattaa gaaagaagga attaattttg
     1141 aattctagaa ttttattatc aagtcctcat atgggaggaa cagaagaaaa atatgttgct
     1201 aaggctttta aagataattg gatagcgcca ttaggtgaaa atgtggataa atttgaagaa
     1261 agtattacaa aatatactgg ttctaagggc ggagcttctg cgttaagttc aggtacagct
     1321 gccatacatc ttgccttaag actgcttaat gttggaaaaa acgatattgt ctttgctcct
     1381 tcatttactt ttatagctac agtaaatcct attttatatc aaggggctgt accagtgttc
     1441 attgactcag aacctaatac atggaatatg agccctgttg cactagagag cgcattagaa
     1501 gaagctgaaa gaaacaacca attacctaag gcaatcatcg tagtgaattt atatggtcaa
     1561 atggctgatt atgaacgaat tttagctatt gcaaaccaat ttaatgttcc agtgatagaa
     1621 gatgcagcgg aatcgttagg ttctacttat aagggaattt cgtcaggctt atttgggaca
     1681 attgggatct attcatttaa cggtaataag atcatcacaa cctcaggtgg aggagctcta
     1741 gtgtctagtg ataaaaaata tgttgagcat tcaagatttt tagcaacaca agcaagagat
     1801 caagcaccgt attatcaaca tagcgaaata ggttataatt accgtctaag caatatttct
     1861 gccggtatcg gacgtggaca aatggaagta ttgaatgata gagtagctaa acgaagagaa
     1921 attttcaatc gatataagga agctttttct ggaattgata gaattgaaat gatgccagaa
     1981 gtggatggca tggtgccaaa tcgttggttg tctactttaa cgatcaatga ttataattct
     2041 attgatcaag tagttaacgt aataaataaa ctaaatgaaa aaaacattga aacaagagct
     2101 ttgtggaaac cactacattt gcaaccagta tttcaagggg taaaaatgta ttaccatgat
     2161 aataatctta gtgtgtcaga aaagttattt aatagcggct tatgcttacc ttctggaagt
     2221 aatatgacag atagtgaaca agaatttgtt attgatgagt ttaaaaaaat attcaagtag
     2281 gtgtgatatt ttatgaacat aggaattatt ggtttaggtt ccatggggaa aagaagactt
     2341 agactcattc aagaacactt taatgatatt ttactatgtg gtgttgatac taatgaggcg
     2401 agacgaaagg aagttgaaaa ggaatttgga ataccaactt ataatagtct aacagaggca
     2461 aaaaatttgt ttgaaataaa ttcagttttt gtttgtacat cccctataac ccatgaattt
     2521 attataaaag aggctttaaa tttagattgt aatatct
//
LOCUS       DARBFD010000180         2513 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000180 DARBFD010000000
VERSION     DARBFD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.186"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            38..970
                     /locus_tag="SCK08_002820"
     CDS             38..970
                     /locus_tag="SCK08_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1202640.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
BASE COUNT          920 a          342 c          496 g          755 t
ORIGIN      
        1 atagttccga ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac
       61 ggatgaactt gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc
      121 ccattgcttg aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg
      181 aaaatcagcc ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg
      241 tccgcttgtt ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg
      301 gacacccgat gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat
      361 ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg
      421 taaaccgaat ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga
      481 acgtgaaaag gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat
      541 cgtaggtctg aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat
      601 catcacattg aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg
      661 gtttgaatct gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt
      721 ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg
      781 aacgccgtct caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt
      841 attgaaatct atggatttca attcagtaga tgaatttttt attcaatctg tcgcatctaa
      901 acgaaataat attcctagaa aatcactgga ttatcgaacg attatcgaac acctttggaa
      961 gtatttttga gttacgtaag tattgatgat ctgtctaatt taatttgaca attaagaaag
     1021 tattaacttg ttttgtgaaa aagagaattt gaaaaacgat tggcgaacaa atgctcggta
     1081 ggtatggtat aaatacagag ctaattggtg tgatgaaatc agatattcgc gtgagattca
     1141 gtaaagtagg agttcgaaaa cagctaggtg acaaacagta ttgctttggt acttcggaat
     1201 ggtttatcaa attcttatca acagttcact tctctttatg tttgagtgat gattcgttag
     1261 gttattgctt ggaaagaccg gatgagagta gaagagttta attttataaa gtgaaaagag
     1321 gtaatgtacg atatacgtag ggctgaacca atagaggata agcaaacgat gttacaggcg
     1381 caaaattcat agcttactgt tttggagata acagaagagg aaataatgca gaagtaaggc
     1441 gaaaaaagga cggaagccta acagcatatg aaataaagaa aaatattata actactgata
     1501 aatcggtatt gagcaaagta ctaataggag tcaaaaacat tcataagcta tgagtgtttc
     1561 ttggctactt ttttattttt atgtatctac aaaagtttcc aggggcagaa cctgtgatta
     1621 ttaatggaag aacctcaagt attgtcttaa atcattgtta tgaaaatgga ttaaaagagt
     1681 tcatcaaggt gtgattaaca agttactttt cattacatcg aatttttcag aagttgctta
     1741 tattggtgat gatattaatg atttatcatg tatgacatct gtgaagaagc gggaggaata
     1801 gtagaatgtc caaaaaatgc cacaagaaat gagattgaat tatctgattt tattgtaact
     1861 cacagtggtg gtgacgaatc tgtgatagag tttattgagt ggatattgga atcgatatag
     1921 tgtagtagta cagaaaaatt atcatacaat taatactagg cataagaata ttggcatatt
     1981 ctagagttaa aagtaactat tcgaaaagga taaggttaga attttttgtt ttgccagtgg
     2041 aagaatataa aaagggcatt ggaaattaat atattagaat gctaagcgga gaaaaaaata
     2101 tatgaaaagt acgtccttgt gatgggatgg aaaaatacac ttatttcaaa caacgataat
     2161 ataattatgt gaaccgaagt atactcgctt ctaattcaat cctcaaatcc cgacagtcgc
     2221 gacttcaaaa aagcgtatag ttttaatgta tccccatatg atccgaaaaa tggtaattta
     2281 caattaacag tttgatgatg ttgaagatgt ggaaatgctg aaattaccat gatgagaaac
     2341 acattaatta aagggaatta attaactaga atataatctg attcaatttg aattaagcaa
     2401 aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta agttagaaaa ataaaaaggc
     2461 atttatggta cactcaaatt gtatttccga cgaaagaaaa caaaggagtg taa
//
LOCUS       DARBFD010000181         2422 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000181 DARBFD010000000
VERSION     DARBFD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.187"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            118..2271
                     /locus_tag="SCK08_002821"
     CDS             118..2271
                     /locus_tag="SCK08_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEG1202641.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          825 a          425 c          537 g          635 t
ORIGIN      
        1 agttgatttt atcttgaaat taggtaaaga atttcttgct ccgcccattt tttctttttt
       61 attaagaagt ttttgggtac aatgaagcat gaatgtaaat aggcggaagg ataggaaatg
      121 atgaaagatt ttatgaaaaa aatattaaat acttcttggt tagaaaaaat aagaaaaggt
      181 gcacaagaac ctaagaacaa aacgtttcgc cggtcccatg ttccttttcg cttaaatttt
      241 ctttttttca ttatatttac gttatttgtt gcgttgattg ctagattggg ttatttgcag
      301 atcgtgaacg gcgaagagat ggaggcgcga gtgaagtcta cttctacgat tacggtccaa
      361 gaaagtactc ctcgcggaat gatctacgac tccacaggaa aagctttagt tacgaataaa
      421 gcgaatcagg ccatcacata tacgagagga acacaagtta cggctaaaga tttgcttgaa
      481 atcgcaacga agctgaactc gctgatcgat gttccggtag atgaaaatct gacagataga
      541 gataaaaaag atttttggct ggctgatccc aaacatttga aagaagccac aaaaagactt
      601 tcggcaaaag aaaagcaatt agggaacagc gaacaatata gtgcgatggt caataaagtc
      661 acagctgatg agattaattt aaacgaggag caattaaaaa ttgctaccat ctttaaacgg
      721 atgaatagtg catatgaatt aaacactgtc tatatcaaaa actctggtgt tactgatcaa
      781 gagttagctg ttgtggcaga acgcgcttct gaacttccag gagtttcgac tggcacagac
      841 tggacacgag aatataatgc agcaagttca ctgaaaagta ttcttggcag tgtgacgact
      901 gaaaagcaag gacttccagc agatgaagca gagaaatacc tagcaaaagg ttattctcgg
      961 aatgaccgtg taggacaaag ttatttggaa aaacaatatg aagatgtcct gcaaggaacc
     1021 aaaacccaat atgaagtttc ccttgataat gaaggaaatg tttcgaatca aaaagaaatc
     1081 ttctctgggg aaaaaggttc taatttaatg ttatctatga atgcagaatt ccaatcaaaa
     1141 gtagaagaaa ttttgaaaag gaattatcaa acattgatca acaacggcaa agcacagtat
     1201 tcgcctggtg cttatgcagt ggctatgaat cctcagacag gagaagtgct agcaatgact
     1261 ggtttcagtc atgaacaagg ttccaaagaa atcaccgaga atgctttagg cacgattact
     1321 agcgcctttg atccagggtc tgtagtcaag gctggtactt tgacagcagg ctgggcaagt
     1381 aacgcgatca gcggaaatca agttttgatt gatgagccga ttcgtctgca aggtgcttca
     1441 gaaaaaagct ctgtctttaa tcgaagcgga caagttgctc ttgatgcagt caaagcatcg
     1501 gaattgtctt ctaatacgta tatgatcaaa gtggcgttaa agatgttagg actagactat
     1561 acacctggaa tgggactacc tagtttagat gaagaagcta aagcttatca gcaattgaga
     1621 gactctttca aagagtttgg tcttggtaca acgactggga tcgatttgcc gaatgaatct
     1681 cctgggatct cacgttctgt tgattatatg aagaagttca atgcggataa tggcaaagaa
     1741 tggtatgctc caggtaactt taccgatttg gcatttggac agtttgacac atatacacca
     1801 atccaattag ctcaatatgc ttcaacagtc gctaatggcg ggaaacgagt acagccgcac
     1861 ctagtcaaag cgatctatgg caatgacgaa aatggaaact tgggtgaagt gaagaaagaa
     1921 attggcacaa cagtagaaaa tacggtcaat atttctgcag aaaatatggc gatcctccgt
     1981 gaaggattcc accaagttgt ccatggtacc gatccttaca caacagccaa accattagcc
     2041 agtgcgaaaa tggacctttc cgcaaaaaca gggactgcag aaacggttgc ggaaggtcat
     2101 ccagatattg cgacagtcaa tagtaatatc gtggcatacg gaccaacaga caatccggaa
     2161 attgccatca gtgttgtctt gccgaactta ttagacgaac aagaccacat aaacttgacg
     2221 attgctaaag aaataatgga cgcgtattat gatatgttca tggcaaaata aaaaagaggt
     2281 cgtgaaaaag ccgcttagtt tcgagtatga agaaaaattt tgtgaaaata gcttctcaca
     2341 tttttgctta aatttgactt cataccgaga aactggcttt tgaacaccgt ttactcggca
     2401 aaaaaggttg tgacaaaaat tg
//
LOCUS       DARBFD010000182         2407 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000182 DARBFD010000000
VERSION     DARBFD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.188"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            5..1150
                     /locus_tag="SCK08_002822"
     CDS             5..1150
                     /locus_tag="SCK08_002822"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEG1202642.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="SCK08_002823"
     CDS             1321..1779
                     /locus_tag="SCK08_002823"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEG1202643.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="SCK08_002824"
     CDS             1938..>2407
                     /locus_tag="SCK08_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEG1202644.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DARBFD010000183         2400 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000183 DARBFD010000000
VERSION     DARBFD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.189"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            207..2069
                     /locus_tag="SCK08_002825"
     CDS             207..2069
                     /locus_tag="SCK08_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1202645.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="SCK08_002826"
     CDS             2069..>2400
                     /locus_tag="SCK08_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1202646.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DARBFD010000184         2236 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000184 DARBFD010000000
VERSION     DARBFD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.191"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(32..256)
                     /locus_tag="SCK08_002827"
     CDS             complement(32..256)
                     /locus_tag="SCK08_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEG1202647.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(253..546)
                     /locus_tag="SCK08_002828"
     CDS             complement(253..546)
                     /locus_tag="SCK08_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202648.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(583..720)
                     /locus_tag="SCK08_002829"
     CDS             complement(583..720)
                     /locus_tag="SCK08_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1202649.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(722..1168)
                     /locus_tag="SCK08_002830"
     CDS             complement(722..1168)
                     /locus_tag="SCK08_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202650.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(1165..1314)
                     /locus_tag="SCK08_002831"
     CDS             complement(1165..1314)
                     /locus_tag="SCK08_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202651.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1328..1660)
                     /locus_tag="SCK08_002832"
                     /pseudo
     CDS             complement(1328..1660)
                     /locus_tag="SCK08_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
BASE COUNT          586 a          476 c          383 g          791 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt attcattttc ttctctccct aaacaaagtt ttgatttgtt gcgtgtgttc
      301 caccaatttt tctgtatgtg tatctaatct ttcatcgtgt ttctttagtt cttcatgaat
      361 catcaatcga tctgatttgc tcgattctaa atctttagtc agcaaatcta aattgtggct
      421 tacttttgaa agagtctcag taattttcga gaaagatgca gcaatcggtc taattactaa
      481 taaaatcaaa gaaacgatag cggttattga tcctgctatc attccccatt cccctaaatt
      541 aatcatgtga caactccttt accttaaata aaaacgcatc aattaagatg cgctcttatc
      601 tttattaatg attttatctg cttcttcgtc tgtaatgcat agtggaacga atagtcgaac
      661 ttgatcgtca gtaaaacagc cccaatcata catcattttc acatcgctaa aactaaacat
      721 actactcacc tccttttgaa gctggattta gttgctcttt aatttctgaa atgtctttgc
      781 tattttgtaa cgaagcaagc atcatttttg aattgatttg tgctaaactg tcagcttttt
      841 ctttcaatgc agtattttcc tgtttaatcg ctacatcgct tagcatgagt ttggcattta
      901 gctgttttag gttgccgttt tcatgttcaa gagcctcgta catcgctttg agattgttta
      961 aatcgttgtg atctaacgcg ttcgctaaaa taatccattg gttcaattta ggatcaaaca
     1021 tctgatcagc aatcgttagc ggttcgccat ctgaacgaac cccttcgagc ggtggctgat
     1081 ctgtgtaagg aacggataca agcatgtcgt ccaatacttt tcctgcatac tctccgccag
     1141 tacggccata tttccaaatg tttttcattt atttccctcc cagtaattga atttcggttt
     1201 ccaatttgga atcggtggtt cgacttctgt gcattcttcc ggtaaatgtt cttcgtcatt
     1261 cacaatgatt tgctcgaatc cgtaaggctt gattgatttg taaactactt tcattagatt
     1321 tcctccctta aattgaataa gtgataacaa atgaataagc tgatccataa cttgagtttc
     1381 ttcgccattt aatggctcca tctgcaccaa tagataactg agcactgttc aaagtagaac
     1441 ggtctatcga gccaaccagt tgctcaaaac taattggtgg ccgatagcct tctggaattg
     1501 ttagtatcgt tgaatcattt ccaccactgc ttttcccatt caacgcaaaa aaataaatag
     1561 tgacggtttt tccttcacga taaagctttg ctgatccggt attcccgttt gtaactgtta
     1621 aggttacgac ttcatatttg ttgtcgtctg atgtcaatac agattttcct tttgattgaa
     1681 taccatcttc gaaatttttc aatccttcga tggattgcgg ttcagtcaaa ctaaccgtat
     1741 cattcaagcc tttttcagta tattcaggcg tgatgtccca gctgtagtcg ttcggattgt
     1801 tgctatcttt caatccttca ccaaagtatt taaactgact aatattcggg gttcgggtgt
     1861 cgcctttttc aattttgagc cagtcaattt ggcatgcgcc ggccgtttct ttaggcaact
     1921 gaaaaatgcg aaggtcttta ggcaaacccg gttcaagttt cgttggtgtg aatgttagag
     1981 accatacgtc tgtcaatccc tcaactggtt ttagttctcc aaaattaacg ttccaataat
     2041 tatatgctac aaaggtttga cttgcgggtt ttgttccttt aagcgtgata gtatacgttt
     2101 gacctattat aagctcttct tccgtgttac ctttatatat ttcgtacgcg ctagatttaa
     2161 ttgggaacgt cgtaccttta tttgcaatat tctcacccaa cgatgtttta cctacccagt
     2221 aagggtcatc tagtaa
//
LOCUS       DARBFD010000185         2233 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000185 DARBFD010000000
VERSION     DARBFD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.192"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..544
                     /locus_tag="SCK08_002833"
     CDS             <1..544
                     /locus_tag="SCK08_002833"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1202652.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="SCK08_002834"
     CDS             complement(900..1472)
                     /locus_tag="SCK08_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1202653.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="SCK08_002835"
     CDS             complement(1488..2093)
                     /locus_tag="SCK08_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEG1202654.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt cta
//
LOCUS       DARBFD010000186         2060 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000186 DARBFD010000000
VERSION     DARBFD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.194"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1008
                     /locus_tag="SCK08_002836"
     CDS             <1..1008
                     /locus_tag="SCK08_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202655.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="SCK08_002837"
     CDS             1025..1174
                     /locus_tag="SCK08_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202656.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="SCK08_002838"
     CDS             1171..1617
                     /locus_tag="SCK08_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202657.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="SCK08_002839"
     CDS             1619..1756
                     /locus_tag="SCK08_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1202658.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2060
                     /locus_tag="SCK08_002840"
     CDS             1794..>2060
                     /locus_tag="SCK08_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202659.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIK"
BASE COUNT          752 a          357 c          425 g          526 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa
//
LOCUS       DARBFD010000187         2058 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000187 DARBFD010000000
VERSION     DARBFD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.195"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(505..870)
                     /locus_tag="SCK08_002841"
     CDS             complement(505..870)
                     /locus_tag="SCK08_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202660.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(946..1191)
                     /locus_tag="SCK08_002842"
     CDS             complement(946..1191)
                     /locus_tag="SCK08_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1202661.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1261..1515)
                     /locus_tag="SCK08_002843"
     CDS             complement(1261..1515)
                     /locus_tag="SCK08_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202662.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1679..2002)
                     /locus_tag="SCK08_002844"
     CDS             complement(1679..2002)
                     /locus_tag="SCK08_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202663.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          771 a          355 c          361 g          571 t
ORIGIN      
        1 gggaacctta tgataaaaaa actattttta ctatcaatat taacgctagc aatggcaggt
       61 tgttctgact ctggcttagc aactgaactg atcacaaaat caactgaagc cacgacagag
      121 gagttaaaat gtggaaacga tcacagtgac tacagaggct taaatcgacg atgcatggga
      181 atattaggca gaagcaattg taagcatcga tgtagtaatc gaagaaggtg tagaagaagc
      241 aacagaaaaa tcaaaagaaa gatcaaaaga agcaatctgt gctgtgcatc gtatacagac
      301 gtcgaagatg cggtactttt aaaccacgtc aggagatgtt catttatgtc tagggaagtt
      361 ggcgatgaac caaaactaga gtacgctttt agttttgcac gtgaggatga agataaccct
      421 aaaacttacg acattttagc ttgatctaag atggttaaag ctttttaatg aagtaagagg
      481 agatagaagg cttttctagt tatattaggt aagataaatt cctttttgct ccaaatttgc
      541 taaacattct gctagataaa cttcatcgtg ttccggataa atcggcacgt tgcgctctac
      601 agtaggtaaa tcaaaaaatg cttcgcatga tttgccacga tacataggat ttttccactc
      661 tggtgctggg ctgtaaccac gtttttccat ttcatccatg acaagctgat gatattggaa
      721 taatctataa ggagaatagt gaaaaacata atccacagta gcatgtttct ttccccagcc
      781 atttcctctt aatgcacagc attcccgatg ttgtcctaaa agctgttgac gaggaagctt
      841 tgaaatcaat ttttcatgcc ataatcgcat agaaatgtct cctttatata tagattgtta
      901 accttagacg ttaaatgaaa aaaccagcac caaggctgga aaaaatcact tcagtttgct
      961 tgctgtttct gagcgaccaa ttaggtaatc aacggaaata tcgtaaaagt cagcgatata
     1021 gattaattca atgatgcttg gaatttcttc tccttcttcg tacttacgat atttgaataa
     1081 aggaatactt aacgcgctag aaacagctac ttgagataaa gatcggccag ttcttaagaa
     1141 attcagacga caacgtaaag tatccagtaa aatagcttct tcgatactca tttaatcacc
     1201 tttcattata gtagaatagt tataatgact atagaaagct acacatgcaa tcaatagtac
     1261 tcaatgtttt ttatttaaac gtatcaaata aagaatgacg atcaataaag acaaaaccag
     1321 tcctgataat tcttctataa aaacactatc gggttcaaag tagttgtaat taaaaacttt
     1381 ccacataaag tagatagccg gaagagtcaa aatagctaaa taaaaccgtt ctttgttcca
     1441 attcataaga taaacaatac ttgcgaccag tgcgaccgca ggcacccaaa acaaaactct
     1501 caccaacgta atcatgtaac ggactcctca acgattcata taataaaaga taacatgtta
     1561 ttgcatacat cacaaatgaa atgctaggaa aataaaagaa gaatagatca atgaaaaatg
     1621 aaaaagcaga acagttttct gctgataagc aggaactgtt ccgctgaatg tgtcttatct
     1681 atcgagaaaa aatcaatgga ttgatatcta agtaattcaa tacatataaa aagaggacga
     1741 tcgcattcac taaaaaggaa agaatgatga tcccatagcg aatggaaaca tatttaacaa
     1801 atacttttgt ttgaatacgg gcatatcttt ttcctttata gctgagcatc atgacaagaa
     1861 gcaaaagggt aatcacacct tgaaaaatca ttccaataaa cagaattcct gaggtttgat
     1921 aatttaattg gagaaaataa gctgctgccg caactaaatc gaataaaatc gcaataaatg
     1981 gcaaaataat cactcctaac attgagaata aataaaagaa taacattttt tctaaaataa
     2041 gcaaataaaa attaaagt
//
LOCUS       DARBFD010000188         2058 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000188 DARBFD010000000
VERSION     DARBFD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.196"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..337)
                     /locus_tag="SCK08_002845"
     CDS             complement(<1..337)
                     /locus_tag="SCK08_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1202664.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="SCK08_002846"
     CDS             complement(404..559)
                     /locus_tag="SCK08_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1202665.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="SCK08_002847"
     CDS             1176..1397
                     /locus_tag="SCK08_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202666.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="SCK08_002848"
     CDS             1398..1655
                     /locus_tag="SCK08_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202667.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="SCK08_002849"
     CDS             complement(1791..>2058)
                     /locus_tag="SCK08_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1202668.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DARBFD010000189         2049 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000189 DARBFD010000000
VERSION     DARBFD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.197"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..267
                     /locus_tag="SCK08_002850"
     CDS             <1..267
                     /locus_tag="SCK08_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202669.1"
                     /translation="QEKYEQVKKDVQYFKDTQTEEACCNLFSDTIPFSTSEQERKERQ
                     QSVTYERYETLLQQATNIQNPTKKETALNYLESVKEWLDQQQQN"
     gene            complement(388..474)
                     /locus_tag="SCK08_002851"
     CDS             complement(388..474)
                     /locus_tag="SCK08_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1202670.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            1150..>2049
                     /locus_tag="SCK08_002852"
     CDS             1150..>2049
                     /locus_tag="SCK08_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1202671.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALS"
BASE COUNT          730 a          364 c          361 g          594 t
ORIGIN      
        1 caagaaaaat atgagcaagt aaaaaaagac gtgcaatatt ttaaagatac gcagacagaa
       61 gaagcttgtt gtaacttatt tagcgataca atcccttttt caaccagtga acaagaacga
      121 aaagaacggc aacaatcggt cacatacgaa cgttatgaaa cactgcttca gcaagcaaca
      181 aacatacaaa atccaacgaa aaaagaaacg gcattaaatt atctagaaag tgtcaaggaa
      241 tggttggatc agcaacaaca aaattgaatt tgattagcaa tcatgttaaa ctgaacctgc
      301 aaatgatcaa acattagata cacgaaagcc ccagagaggt cggattctct ggggcttttt
      361 ttcaggttga tgtcgccttt attgatttta cttcttgttt cggttattca accaataact
      421 gaataaagag acacaaactt ctacaagaag ggggcaaatg aaccgttcaa acattagata
      481 cacctccttc cagtcgcaag actgtcggat aggcgacaag ttattatacc ttacaaaata
      541 aaatctgtgt ggtgttttta agtatccctt attttcgggt tgttagggct tgccctgacc
      601 gcctgtaagg cgctttaaga acaagataag gttttttacc caagactgtc ttaaaacgac
      661 ttagacgagc tatcagaggc ttcccccacc tccgttactg acttgttttt agcaaaggag
      721 cctttaggcg actgcgcagt acaatgtgag agcatagcga tcacactttt ttagcgaccg
      781 tagggagcaa ggttttgaat tggcagtttt gaaaaatgcg cgaacctgtt ggtagaaaaa
      841 agaagtggtt tttaggcggg aaattttgtc accctctttt ataatctctt tttaaacctt
      901 tttttaaacc tctttaccgt cggctgtgtc cttactcctt caatggatac agcgttttat
      961 aaactacatt ttgtctgctt ttaaactaca ttttgtctgc ttttaaacta cattttgtct
     1021 gcttttaaac tacattttgt ctgcttttaa actctttttt tgcactcacg tatgttataa
     1081 tatgcattat cacagacaaa acgttgttta taacgtctgt atccattgaa ggagtaagga
     1141 ggaatatctt tggcaaatga aatcgtgaaa taccatcatg aattaaatac cattccgttg
     1201 cggaaattta caccagttga aatgaatcta tttttctcta ttgtgtctag aatgcgcgaa
     1261 aaaggcgata aaacagtccg tcttactttt gaccaactaa aagatttaag taattataaa
     1321 gcaactgcta ataatcgttt cattaatgat ttagaaatga cttatgataa attaatggat
     1381 ttacgttttg gtaaaagaag tgctaatggg ctcgatatag atagattcgt tatgtttagt
     1441 gagtttaaaa tacgtggaac tgctaaagat ccatatgtag acatacaagt ttacgaaaaa
     1501 gctttacctt tactaaacaa tttagatgag tgggttcgct attcactcca acaatttaac
     1561 gaattacaaa gtagctatgc aaagactatg tttcgattac taaaacaata tcgaactaaa
     1621 ggttttgctt atttttctaa agaggatttt catgaactat tagatattcc aaaaagttac
     1681 tggaacaacc aagcaaacgt tgataatcgt gttataaagc ctatacgaca ggaattaacc
     1741 gcaatcttca aaggattatc aatcaaaaag aaatacggaa aagggcgagg aaaacctgtc
     1801 gttggctatc aatttacgtt taaaccagaa atcaaaaaag cagatgattt taataaacat
     1861 gcaccaacca aagaagaaca aaataaagca ttggctttag ctgataaaca aaaagaatac
     1921 gaagaacctg ctacaacaat taacgaaaaa atcaaacaga agaaacgaga acttgaactc
     1981 ctccaacaaa tcaaacaact agaaaaagag aaagaagctc ttgaaaaaca aacagagtcc
     2041 gcactttcg
//
LOCUS       DARBFD010000190         2004 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000190 DARBFD010000000
VERSION     DARBFD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.198"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..894)
                     /locus_tag="SCK08_002853"
     CDS             complement(<1..894)
                     /locus_tag="SCK08_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1202672.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALS"
     gene            1531..1617
                     /locus_tag="SCK08_002854"
     CDS             1531..1617
                     /locus_tag="SCK08_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1202673.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(1738..>2004)
                     /locus_tag="SCK08_002855"
     CDS             complement(1738..>2004)
                     /locus_tag="SCK08_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202674.1"
                     /translation="QDNYERTKKDVQYFKDTQTEEACYNLFSDTIPFSTSEQERKERQ
                     QSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEWLDQQQQN"
BASE COUNT          563 a          367 c          348 g          726 t
ORIGIN      
        1 cgaaagtgcg gactctgttt gtttttcaag agcttttttc tctttttcta gttgtttgat
       61 ttgttggagg agttcaagtt ctcgtttctt ctgtttgatt ttttcgttaa ttgttgtagc
      121 gggttcttcg tattcttttt gtttatcagc taaagccaat gctttatttt ggtcttcttt
      181 ggttggtgca tgtttgttaa agtcgtctgc ttttttgatc tcaggtttga acgtaaattg
      241 atagccaata acaggttttc cccgtccttt tccgtatttc tttttgattg ttagtccttt
      301 gaagattgca gttagttctt gacgtatagg ttttataact cgtttatcta tatcaggttg
      361 cttataactt ttaggaatat ctaataattc aagaaagtct tctttggaga aatatgcgaa
      421 acctttggtt ctgtattgct ttaacagacg aaacatagtc tttgcatagc cactttgtag
      481 ctcgttgaat tgttgtaaag aatagcgtac ccactcgtct aaattattta acaaaggcag
      541 tgctttttcg taaattcgta tatctacgta aggattttct acgtccccgt tgattttaaa
      601 ttctgtaaac attacaaaca tatcgtaaga aagcccagac tcacttctac gaccaaagcg
      661 taaagataag attttttcat aagttgcttt taaatcttct acaaatcgta catttgcagt
      721 tgctttgtag ttacttaaat cttttagttg gtcaaacgta aaacgaactg tcttatcccc
      781 tttttcacgc attctagaca cgatggaaaa aaacaaattc atttcaaccg gtgtaaattt
      841 tcttaaagga attgtattta attcatggtg gtattttaca atctcattag ccatataatc
      901 acctcaatcc aattatgaat gacatttttt aaatgtcaag acattcatat ttgacgttca
      961 aacagacaaa atgtccttca taaacagaca aaatgtcctt cataaacaga caaaatgtcc
     1021 ttcataaaca gacaaaatgt ccttcataac attctgtatc cattggtaga gtaaggacac
     1081 agcttacggt aaagaggttt aaaaagaggt ttaaaaagag atataaaaag aggggggcta
     1141 attttcccgc ctaaaaacca cttctttttt ctttaaacag gttcgcgcat ttttcaaaac
     1201 tgccaatcca aaaccttgct tcctacggtc gccaaaaagg tgtgatcgct acgctctcac
     1261 attgtactgc gcagtcgcct aaaggctcct ttgctaaaaa catgttggta aagaggcctt
     1321 tgagcggttc tgaatgctta tctaagccgt tttaagacag tcttggataa aaatccttat
     1381 ctagctttaa agcgccttac aggcggtcag ggcaagccct aacaacccta aaataagcga
     1441 tacttaaaaa caccacacaa gtttaatttt ttaagatata ataacttgtc gcctatccga
     1501 cagtcttgcg actggaagga ggtgtatcta atgtttgaat tgttcatttg cccccttctt
     1561 atagaagttt gtgtctcttt attcagttat tggttgaata accgaaacaa gaagtaaaat
     1621 caataaaggc gacatcaacc tgaaaaaaag ccccagagaa tccgacctct ctggggcttt
     1681 cgtgtatcta atgtttgatc atttgcatgt ttagtttaac atgattgccg atcaaattca
     1741 attttgctgt tgctgatcta accattcctt gacacttttt agataattta atgccgtttc
     1801 ttttttcgtt ggattttgta tgttcgttgc ttgctgaagc agtgctttat aacgttcgta
     1861 ggtaactgat tgttgccgtt cttttcgttc ctgttcacta gttgaaaaag ggattgtatc
     1921 actgaataaa ttataacaag cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt
     1981 tttggttcgc tcatagttgt cttg
//
LOCUS       DARBFD010000191         2003 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000191 DARBFD010000000
VERSION     DARBFD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.199"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..378
                     /locus_tag="SCK08_002856"
     CDS             <1..378
                     /locus_tag="SCK08_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1202675.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="SCK08_002857"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="SCK08_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1202676.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="SCK08_002858"
     CDS             1859..>2003
                     /locus_tag="SCK08_002858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202677.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DARBFD010000192         1992 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000192 DARBFD010000000
VERSION     DARBFD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.200"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="SCK08_002859"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="SCK08_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1202678.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="SCK08_002860"
     CDS             853..1041
                     /locus_tag="SCK08_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1202679.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="SCK08_002861"
     CDS             1054..1611
                     /locus_tag="SCK08_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1202680.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="SCK08_002862"
     CDS             1643..1885
                     /locus_tag="SCK08_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1202681.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          725 a          308 c          406 g          553 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt tatatgaagt
     1981 attctttatt cc
//
LOCUS       DARBFD010000193         1907 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000193 DARBFD010000000
VERSION     DARBFD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.201"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            259..855
                     /locus_tag="SCK08_002863"
     CDS             259..855
                     /locus_tag="SCK08_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1202682.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(913..1395)
                     /locus_tag="SCK08_002864"
     CDS             complement(913..1395)
                     /locus_tag="SCK08_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202683.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1501..1698)
                     /locus_tag="SCK08_002865"
     CDS             complement(1501..1698)
                     /locus_tag="SCK08_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202684.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          656 a          373 c          282 g          596 t
ORIGIN      
        1 tcataaaaaa aagaagatag agatttaata attatatatc agctgttttt ttcaaataag
       61 caaaaattcc taaaacacaa caatgaaagc gataactaag aaaatcctta cataacgatt
      121 tttcttatta aaattgtttt ttgaacttcg cttatttttt gtgagcatat ctgtttactt
      181 actttttgta aggtgctata cttcatttcg taatcaatta aaaaaatgca aaacgcaaat
      241 caaaggagaa attttattat gacaactttt actgacactt tgaaaaaccg ccgttcaatc
      301 tatcatctag gacgtaatgt ttcactttct aatgaagaat taacaacttt gatcaaagaa
      361 gcaattaaag aaagcccaac tgctttcaat gctcaatcaa cacgcgcagt tatccttttc
      421 ggagatgcac atgaaaaact ttgggaaatc actgaagaag cacttcgccc attgactcca
      481 gcagaagctt tcccaaatac acaaaacaaa ttagctggtt tcaaaaatgg ttatggtaca
      541 gtattattct tcaaagatac tgacgtggta aaaggtctac aagaacaatt tgaattatat
      601 gcagacaact tcccagattg gtcagaacaa tcaaacggaa tcgcaacagc taatacttgg
      661 gtagcattag ttgataaagg attgggcgca aacttgcaac attacaaccc agttattgac
      721 gaagcagttg caaaagaatg gaatatccct tcaaactgga aactacgttc gcaattagta
      781 ttcggttcac cagaaacacc ggctggggaa aaagaataca tgaacgatgc cgatcgcttc
      841 cgcgtatttg gataatttta tttgaaataa aaaacagagg ttgtgacaaa catcttgtcg
      901 caacctctgt ttttattctg gatcaatcat aatatcaatc tctgcctcat cgtttaagcg
      961 ttgggacaaa aatggaacta attcttctaa tgatgcagaa aatttcagca tcggtcctcg
     1021 ccatccgttt cggaaaaaat tcatttttac gattttagaa ccttttggct gatataatac
     1081 aattttattg atctcagaaa aaggaacacc ttttttgtca aagctattca aaacaagtcc
     1141 ttcttctgtg attcctctac tgtccaacaa aaaactaata aaaatcattg ctgacaagat
     1201 cccttttgcc aattcatctg ctgtaggatg actgatgagt gtataccaaa tgactagtga
     1261 cactaagaag ggtatcacca agtagaccca tcgatttcgc acagtccgat aaataatttc
     1321 ttttcgaacc agccaaagaa ataaaaacat cacaacaacg aataaaaaca atagaaccgt
     1381 aaagaatgtt accatgacaa ctcgcctcgc tttttcttct ccagaaaata ggaaaagaat
     1441 actgatttac cttctcaaag aaattggact tgcagcacgc cgcacgtcca attgttttct
     1501 ttattcaaat gtatcatatt ttgcaacaac catgcttgct ttttcatcta ataattcttg
     1561 gtcctctgca gcacattggg caactttgac cacggcgcag tttgtcattt ttgatacaac
     1621 ttcaccaatg accgtttttg taaaaccaat tggtttgcat gcatattgtt ctcctaaatt
     1681 aatcatttct tccgtcatca aaatcctcct aaactgctgg aacttttctc cacattacca
     1741 ctgctatttc tttggagatt taactcattg atttttattt acagttaaaa atcaacctta
     1801 caggcattat agcgtactgt tttcgttttc acaattaaaa cagctgttgt atttagaaga
     1861 atttttttca aaaaattaaa acatattcct taaaaactaa ttatttc
//
LOCUS       DARBFD010000194         1796 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000194 DARBFD010000000
VERSION     DARBFD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.202"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="SCK08_002866"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="SCK08_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1202685.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DARBFD010000195         1786 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000195 DARBFD010000000
VERSION     DARBFD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.203"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            52..1749
                     /locus_tag="SCK08_002867"
     CDS             52..1749
                     /locus_tag="SCK08_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202686.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT          577 a          338 c          306 g          565 t
ORIGIN      
        1 ggaattgctt atttctttta ttttattttt tctcctgcgg acagcaatca aatgaaagaa
       61 ataaaagatt cgtgggtttt ctatacaaaa gatgatcctg attttaagtt tgacagcaag
      121 tacactcgta cgattcctac cgtggataaa gacgaaacat tcattatgga aacgacactt
      181 gaaaaaccgt tggacgaagc aaatttacta atcaaaggaa accatcaatg gattactgta
      241 tatctaggcg aaaagatttt ataccaacgg aaagattatc aggaagaaag caatcccgga
      301 ctctcattgg ctgtgataga tttaccagca aattatatcg gaaaaaaatt gacccttaca
      361 gtctcatctc cttatcagaa ctatgctgga ctgccaccga aagtttatct aggaacaact
      421 ggaccattga ttagttttat ttattcacaa tcagtgccgc aagtattgac gatgataatc
      481 gctgttttta tcgctatagg gacctttttc tatacgatct acttgatgta taaacaaaaa
      541 cgtcttgaat actcacttat tctcttaagt tgctttgctt tggcattagg atttgaagct
      601 gtatcagaag atattttaag tggtattctt tttgaaccat tcgttcattc ttttttatct
      661 catttattta ttatcttaac gtcagctttc atcatctgct actattggag ccgaatgttt
      721 tactataaaa aatggtacgg aattttttgt acgttccaac ttttgatttt tatcggcgtt
      781 ctgctttatg caacttttac tcctgcagaa cttcctgaat taatgccgat tgctaatatt
      841 gctagtgtta taagcacttt agtcacttca cttgcatgca ttggcgaagc atataagaat
      901 aaccgttttt atgtagcttg tacaccttgg atcgtcttgg tagccattat ccattgcttg
      961 atttacatcc aaacggcatt aggtatctat caaacaacga tcaattggtc tacgatttta
     1021 tatattatca ttctaatcgt tattatcagc tacaatctaa tcgatcacat cttaaatacc
     1081 gacaaagatc ggagacaagt agatttctta aaaatcaaaa atgatttgtt ggagcaacat
     1141 tatgagcagc ttcgacagca tatccaagaa gtaaatacac ttcgcctgga atttgtccaa
     1201 gagatggaga atctgcaaga tttaatgcat acggaccaaa caaaagctaa aaactatgtg
     1261 gaaaagatat tagaagaagc aaaaaatttc gaaatgctgt gttctttttg caaccaccag
     1321 gtgacaaatc ttatcttagc gcgttaccag aagttagctg ccaaacggaa tattcagata
     1381 accttccaag cagatcttcc cgaagaactt cctatcaacg atgacgatct ggctcaactt
     1441 ttgatccacg ctttagagca ttcctttaga gaaactcatg ccatcgaaaa tccactgtat
     1501 cgaaaaattt atttgtcgat acacgaacaa gaggaacatt tcgtcatttg ttgcgaacat
     1561 agtgcacatt acgatacaaa cctatttagt cgaggcatca cagaagaatt agatgatcag
     1621 gaacgttttg acttaatgat gatgaaagac gtagcggatc gttacacagg aacactaacg
     1681 caggaaaaag atactttcat tgatcggatc actgttcaac tctctcgaaa ttacccgaat
     1741 cctgtataaa aaatagcgga atccatatat gtggattccg ctattt
//
LOCUS       DARBFD010000196         1760 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000196 DARBFD010000000
VERSION     DARBFD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.205"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(34..480)
                     /locus_tag="SCK08_002868"
     CDS             complement(34..480)
                     /locus_tag="SCK08_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1202687.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(716..1642)
                     /gene="cysK"
                     /locus_tag="SCK08_002869"
     CDS             complement(716..1642)
                     /gene="cysK"
                     /locus_tag="SCK08_002869"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEG1202688.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT          496 a          395 c          266 g          603 t
ORIGIN      
        1 tttttttagg tataacactt gtagaaactg tttttacatt tgcgcctttt ttgctgactc
       61 tttacaatcc ggacagagtc catacaattc caaccgatgt tcattaatct caaaatcagt
      121 cagtctgctt gcagccattt ctatatcttc aagccctggg taatgaaaat caacaatctt
      181 accgcagttt tcacaaatta catgataatg acgctttgaa ctgaaatcaa atcggctaga
      241 agcatcgccg tacttcattt cttgaacaaa tccaatcgaa gtaaataagc gaaggttatt
      301 gtaaacagtc gcaacactca tatttggaaa ccgatgttca agtgctttat aaatctcatc
      361 agccgttgga tgggaatgat gttcaatcaa atattctaaa accgcgtacc gttgaggtgt
      421 gattcgaatg ttctcttttt ttaacttttc gataacctct tcgactaact tattttccat
      481 gtaaacaccc cacgttgtta ttctcgtctt atcatactat ttttacattt taaaatccac
      541 ttaacatgaa aagaaaattc aattttattg tattttaatg aaaaaagacg taaaacagtc
      601 gcgttcacta ttctacgtct ctattcaaat aatttttctc atcataaatt tttgtgtgtt
      661 cttttcatct ttaaaagttt tttacataaa cctatctgga gaaaaaccaa gtgccttata
      721 tctcttggta aagggctgta gataaataac gttcaccatt gtcgggaacc acagctaata
      781 ccttcttgcc ttttcctaat tcttttgcga cttttagcgc ggcagcaatt gcagcgccag
      841 atgagatgcc tactaaaatc ccttcttttc gcccaacttc tctagctgtt tccatcgctt
      901 cgtctccact aatagaaagg actttatcat acacagaagt atccagtgtt ttaggaacaa
      961 aacctgtacc gattccttgg attttgtgag gtcccgcttc tttcccttct aaaatagcag
     1021 attctgctgg ttctaccccg attaattcga ttttcggata cacacgtttt aattctcggc
     1081 cagcgccagt gatcgttcct cctgtaccga tacctgcaac aaacgcgtcc aagccattta
     1141 ctccaaatgc ttgatgtatc tcaggaccag tcgtcttctc atgcacaaga ggatttgctt
     1201 cattttcaaa ttgtaatggt aaaaagtagc cgttttcttt tgccaaacgt tctgcttctc
     1261 tgattgatcc agaaatacca tccgcaccag gagtcaaaat caactctgcg ccgtaccctt
     1321 tcatcaataa acgtctttcg atactcatcg tctcaggcat cacgatgatt actttatatc
     1381 cttttgcaac tcctaccatc gacaatccga ttcctgtatt tccagaagta ggttcaatga
     1441 tagtatcacc tggtttcagt agaccatcgt gttctgcttt ttctatcata cttaaagcaa
     1501 tacgatcttt tacacttcct cctggattaa aaaactcaag ctttacaaaa acatctgctg
     1561 aatcttctgg tacgatttta tttaatttga cgattggcgt tttgccaatc aattcggtga
     1621 ctgagttata gatagctgtc atcttcaatt cctccttgtc tttgctcttc ttacttatca
     1681 taatacaatc tgttcattta tggcgagtga ttcgtctttg actagcaaaa aaaagacaaa
     1741 gatataccat atatggtaat
//
LOCUS       DARBFD010000197         1739 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000197 DARBFD010000000
VERSION     DARBFD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.206"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..1235
                     /gene="aph(2'')-I"
                     /locus_tag="SCK08_002870"
                     /pseudo
     CDS             1..1235
                     /gene="aph(2'')-I"
                     /locus_tag="SCK08_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1219..1611
                     /locus_tag="SCK08_002871"
     CDS             1219..1611
                     /locus_tag="SCK08_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1202689.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          716 a          133 c          297 g          593 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      601 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      661 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      721 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      781 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
      841 ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa
      901 ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt
      961 ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta ttgttagatg
     1021 gcaataatag attaactgga ataattgatt ttggagattc tggaattata gatgaatatt
     1081 gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat tttggagaag
     1141 atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat caagatatag
     1201 ttgaagaata ttatcctatt gaaactattg tttattgatg ataatatggt ttttgatatt
     1261 gataatttaa aaggatttct taatgatacc agttcatttg ggtttatagc taaagaaaat
     1321 aataaaatta taggatttgc atattgctat acacttttaa gacctgatgg aaaaacaatg
     1381 ttttatttac actcaatagg aatgttacct aactatcaag acaaaggtta tggttcaaaa
     1441 ttattatctt ttattaagga atattctaaa gagattggtt gttctgaaat gtttttaata
     1501 actgataaag gtaatcctag agcttgccat gtatatgaaa aattaggtgg taaaaatgat
     1561 tataaagatg aaatagtata tgtatatgat tatgaaaaag gtgataaata aatgaatata
     1621 gttgaaaatg aaatatgtat aagaacttta atagatgatg attttccttt gatgttaaaa
     1681 tggttaactg atgaaagagt attagaattt tatgatggta gagataaaaa atatacatt
//
LOCUS       DARBFD010000198         1676 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000198 DARBFD010000000
VERSION     DARBFD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.207"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            316..570
                     /locus_tag="SCK08_002872"
     CDS             316..570
                     /locus_tag="SCK08_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1202690.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="SCK08_002873"
     CDS             571..888
                     /locus_tag="SCK08_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1202691.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1522..>1676)
                     /locus_tag="SCK08_002874"
     CDS             complement(1522..>1676)
                     /locus_tag="SCK08_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG1202692.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
BASE COUNT          588 a          263 c          317 g          508 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 aacccgtggt gctagttaat tttaatatcc taaagaatat ctcaaagttc cttcaaaacg
     1561 tcgttgtgct cgacttaata gaacactgta agccagtaat atgctttcaa atctactctc
     1621 tagcccttta acagaacgtg agttgaaatt ttgacaacct aatttttcta aggaag
//
LOCUS       DARBFD010000199         1672 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000199 DARBFD010000000
VERSION     DARBFD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.208"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            75..1622
                     /locus_tag="SCK08_002875"
     CDS             75..1622
                     /locus_tag="SCK08_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEG1202693.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT          560 a          305 c          343 g          464 t
ORIGIN      
        1 tgaaccccta tttcttgtgc gtttcttgtg gtattctgta acaaaatcag ttcgattcaa
       61 ggtggtgaaa aattttggaa actacagata cgttgcttca gttgcttcaa gaagcccata
      121 aaaccaatca agcacaacaa caaaccattc agaatcttac aactgaaatc caactattga
      181 atgaaaaggt caattattta accaacaaat tatttggacg ctctaaagag actctctttg
      241 aggaaacgaa tgggcaattg aatcttttta gtgatgaaga aatttctgta agtgttccag
      301 aagcagctgc caccattatt ccagtaaaag gacatcaacg agttgtagga acgaaagcag
      361 acaaaatcaa acacttacct ataacagaga aagaacatct ccttcctttg gaagaacaat
      421 tttgtgaaca ttgcggttca cagatgaaag atatcggacg gacaaaagta cgagaagaaa
      481 ttcgtttcca tcaggctatg ttggattgtc tcacccatta tcaacatacc tattgttgta
      541 aaagctgcga aaaagaagga ctttcttctt ttaaaaaagc gatcgttcct aaacctttga
      601 tttctaatag cttaggttcc aatagtttag tggcggaaac gatccggatg aaatttggtc
      661 agaaagttcc tgcctatcgg caagagaact attggaaaca aacacatggg ttggatattt
      721 cgagagataa tatcaccaat tggcatatta aagcagtgca aaacgcgtta gaccctttag
      781 gagaacgatt aagagtctat cttaatcaag aagagattct acacggggat gaaacaagtt
      841 atcgtgtgat tgaaagtgcc aaaacagata cctattactg gcagttttgt acaggaaaag
      901 acagtcagca tcccatcgtt tattatcacc acgacgaaag tcgtgctgga gatgttccga
      961 aaacttttct caaggagttt acagggtatt tacactgtga cggctatagc gggtataatg
     1021 ccgtggaaag tgtacgactt gtctattgtt tcgcgcatgt tcggcggaaa tttttcgaag
     1081 caattccaaa aggaaagaaa aacacggata ttcccgcagc tcaagcagtc aaacaattgg
     1141 ataaatggtt tgtcctagaa aagaaatgga aagacttttc tcctgagaag cgattaagct
     1201 gtcggcaaca agaactgcgt ccattattta ttgcttttta tgagtggatg gcaacgatag
     1261 atcctgttgc aaaatccaag ttggatgcag ccgttcaata tgcgtgtaaa ctacgaagtg
     1321 gttttgaacc tatttttgaa gatggtcgtt tagaactcac aaataatcgt gcggagcgaa
     1381 atatcaaaga gctggtgata ggacggaaaa attggttgca ttccacaagt cttgaaggag
     1441 cacgaacttc aggaatcatt ctgagtgtat ataaaacagc agaacttaac ggattgaatc
     1501 ctgtaaagta tctagagttt ctatttgata agataccaaa tcttcccgta ctttcagcag
     1561 aaactttaga tcaactgttg ccttggaaca aggatgtaca acaacatttt tctcgtaact
     1621 aaaaacgcca aacattcccg attctattct ataggtcggg ttatttgacg ta
//
LOCUS       DARBFD010000200         1655 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000200 DARBFD010000000
VERSION     DARBFD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.209"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..114
                     /gene="mobC"
                     /locus_tag="SCK08_002876"
     CDS             <1..114
                     /gene="mobC"
                     /locus_tag="SCK08_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEG1202694.1"
                     /translation="KWCNTHQHDVAPDDAQRLSENLKIMQKELQKIWQQLK"
     gene            96..1010
                     /locus_tag="SCK08_002877"
     CDS             96..1010
                     /locus_tag="SCK08_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG1202695.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1255..>1655
                     /locus_tag="SCK08_002878"
     CDS             1255..>1655
                     /locus_tag="SCK08_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202696.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLP"
BASE COUNT          645 a          317 c          302 g          391 t
ORIGIN      
        1 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
       61 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      121 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      181 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      241 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      301 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      361 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
      421 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
      481 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
      541 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
      601 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
      661 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
      721 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
      781 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
      841 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
      901 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
      961 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1021 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1081 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1141 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1201 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1261 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1321 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1381 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     1441 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     1501 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     1561 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     1621 atgaaaaaac tcactcgttt ttatcaaaac ttacc
//
LOCUS       DARBFD010000201         1637 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000201 DARBFD010000000
VERSION     DARBFD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.210"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            136..309
                     /locus_tag="SCK08_002879"
     CDS             136..309
                     /locus_tag="SCK08_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202697.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="SCK08_002880"
     CDS             949..1422
                     /locus_tag="SCK08_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEG1202698.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..>1637
                     /locus_tag="SCK08_002881"
     CDS             1431..>1637
                     /locus_tag="SCK08_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1202699.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDS"
BASE COUNT          536 a          262 c          244 g          595 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcg
//
LOCUS       DARBFD010000202         1577 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000202 DARBFD010000000
VERSION     DARBFD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.211"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(24..938)
                     /locus_tag="SCK08_002882"
     CDS             complement(24..938)
                     /locus_tag="SCK08_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202700.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(996..1577)
                     /locus_tag="SCK08_002883"
                     /pseudo
     CDS             complement(996..1577)
                     /locus_tag="SCK08_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          607 a          213 c          216 g          541 t
ORIGIN      
        1 ttgctaaaat atgtgctagc aaatcattca tggcttgttc ttatttttaa ttttatgcaa
       61 taaaaaagaa gaacaattag cgtgattata gaaataaata atgagatttt agaaaaaaat
      121 gtgtatgaat acctgattgt tactttattt attccacttt cactttttac tgtaacaaaa
      181 ggacctcctt gttttagtac tgtagcattt tttgaattaa cagtaaccgt taatccttta
      241 taccggaata caggtaaaac tattgttttg ccttttgaag atttaaacgt aaatgtaatt
      301 tcatctttat ttggagaaaa gctactataa ataacttgac catcttctgt aacttgatgg
      361 tttttcaaaa gcaattgatc ttttttatag cctataggta cataatcaaa attctcaaat
      421 tcttcaagtt tattttccat ttgttcatta gaataagttc cttgaaaatt catttctttc
      481 accatgttaa ctactgatga ataatttaaa attgaaatag ctaatattaa taatagtata
      541 aaatttcttc tccttgtata agaaaatttt tccaactgtg gagaaataaa tagagatact
      601 gaaaaagata tgaacaatgt tgaaaatatc ataaaacgcc aaggagcttg aataatattt
      661 aaaggagtat tttgtaaggc aaaccatgga aatatttttg tacaaagtag aatagatatt
      721 atacccatta tataagtgat tttagataag attcctaatt ttttaaagtt aaatgttagt
      781 ataattaaac aaataagcaa agttaagcca ataccaaatg atagtaattc tgaatctata
      841 cttccaatca aaaactcttt acccagataa attgctcttt tttgtaatat atccacataa
      901 ggtctattca gatcttgata caatgtctgc tcaatcatag gacctataaa cgctagtgaa
      961 agaagtaaag taactatagc agcttttatt aatttaataa ttaagaagat ttctattttc
     1021 tttttccgat aaaattgaat aattaaagca attataatca ttaccatagt catagctaca
     1081 gaaagaacat gagtgtaaat taataaagtc atgctaatcg ccaacataac ccaagattct
     1141 ttttttgagt cgtaaagctt tataaacccg agtaaaataa gaggaagtat tgacattgcg
     1201 agtaattccc ccattgcccc tcgaagaaat atttctacac tcctaaaaga agatgttgta
     1261 tatataatag cgaaaaagga gctagtaaca tgttttttgg taatttcata cattacataa
     1321 tgacatataa atagagtaat gagcgtaaca atataaagaa aaaggttata gcctattgct
     1381 aaattagtaa acttgatcaa taaaaataaa ggatatagag ttaaccatgg gtaaaaatta
     1441 tttacttgca tgccatatga tctaaacgtt tcaaaattaa taggggagct aaaaatagat
     1501 tctaaactta acgcacgatt aatgtgaaat gccgtatcat aaaagccaaa tatttccatg
     1561 tttttaaatg ctggaaa
//
LOCUS       DARBFD010000203         1558 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000203 DARBFD010000000
VERSION     DARBFD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.212"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            43..1509
                     /locus_tag="SCK08_002884"
     CDS             43..1509
                     /locus_tag="SCK08_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEG1202701.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          430 a          290 c          328 g          510 t
ORIGIN      
        1 gtataatatt ttctgaaaat ttaaagaatt ggggtataga ttatggaaaa acagcaagac
       61 aagggcttct tcgggcagcc gaagggattg tcaactttgt tcttcactga gatgtgggaa
      121 cgatttagtt attacggtat gcgtgcatta cttatctatt atatgtacga tacagttgcc
      181 aacgggggac ttggattacc acgagcaaca gcattagcta tcatgtcgat ctacggatct
      241 ttgatctaca tgtctagtat cgtcggcgga tgggtatctg accgagtgtt aggagcaaaa
      301 cgcactgtat tcttcggtgg agttttcatt atgcttggcc acatcgtctt ggctttacca
      361 tttggtgtaa gtacattgtt tgtttcgatg gcattgatta ttatcggaac cgcctttttg
      421 aaaccaaatg tttctggcat ggtcggacat ctttattcaa agactgactt gcgaagagat
      481 gcaggatttt cgatttttta catgggtgtc aacttagggg cattattagc accactaatc
      541 gttggtacaa tcggtcaaga atataactat catttagggt tctctttagc tgctattggg
      601 atgttctttg gattattaca atacatcatc caaggacgca agacattaga cggagtgggt
      661 acaacaccgc cgaatccttt gacaactgaa gaacgtaaag gattcatccg taacttgatc
      721 atcgcacttg ttgttatcgc tgcagtattc ggcggggcac aactaactgg tcatctgaca
      781 attgactttt ttgtcaatac aatcagtgta ttggggatta tcttaccgtt gtattacttt
      841 atcaaaatgc tgacttcaaa agatgtaact gctgaagaaa aaccaagagt atgggcttat
      901 atcccattat tccttgcagc aatcattttc tggtcattac aagaacaagg ttcatctatc
      961 ttagctttat ttgctagtga acgtacacaa tctacgctat ttggtgtaac gatccaagca
     1021 agctggtatc aatcattgaa tccaattttc atctttattc tgacaccagt attcgtaacg
     1081 atgtggacga aacttggtga acgtcaacca tctacagtag tgaaattctc tcttggactt
     1141 ttatttgcag gattatcgta cttgctattg atgttgccgg gtatgcttta tggtacaagt
     1201 ggaaaagtaa gtccattgtg gttgttggga agtttcttca tcgtggttat tgctgaattg
     1261 tgtctttcac cagttggttt gtctgtgaca acaaaattag caccacgagc atttgaatca
     1321 caaacaatcg ctatttggtt cttagctgat gcttcttcac aagcaatcaa tgcacaaatt
     1381 gcacgcttct acacaccggg aacagaatct gcttacttcg gtattatcgg tgcagtagca
     1441 atcgtcggtg gtatcttgct atttactgtg aaagaaccaa tcaaaaaact aatgggctct
     1501 attcattaat caatgaacat aaaaacagga attgcttatt tgcagcagtt cctgtttt
//
LOCUS       DARBFD010000204         1533 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000204 DARBFD010000000
VERSION     DARBFD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.213"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            91..807
                     /locus_tag="SCK08_002885"
     CDS             91..807
                     /locus_tag="SCK08_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1202702.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            826..>1533
                     /locus_tag="SCK08_002886"
     CDS             826..>1533
                     /locus_tag="SCK08_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEG1202703.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNT"
BASE COUNT          589 a          186 c          298 g          460 t
ORIGIN      
        1 ttatagagga agaaacatta tttcaatgat taagagtcga gagcacaaac ggtagacgat
       61 gttattttta taaaaagata taatttatta atggtcgata attatgaaag gaaaaatatt
      121 cttaaaaaaa agagtatagg atgtcgtatg aaaatagtcg ctatagtacc aatgaagtta
      181 aataacaaaa gattgccaca aaaaaacacg aaagctttta ccaatggaaa accattgtgt
      241 tattatatac tttcaacact tttaaaagta aaacagattg ataaagtgta tgtctattgt
      301 agtaacccag atattaaaga atttatacca gaaggtgtaa catttttaaa gagatctagt
      361 tccttagatc aggatacaac gagtattaca caggtattag ccggtttcac aaaagaagta
      421 gaggctgatg tatatgtaat gacacacaca acagcaccat ttattagcga aaaatctatt
      481 gaaaaaggac tagatgccgt gctgacagga gaatatgatt ctgcatttgc tgcaaaaaaa
      541 ttgcaagatt tcttatggaa agacggtgaa ccatttaatt atgaattgga caatattcct
      601 agaacacagg atttgccgcc tttatacgaa gaaactagtg gattttatat ttataatcgc
      661 aatgtgatga ccgagttgca tagaagaatt gggaaaaaac cttgcatagt tgaagtaggt
      721 gagattgagt cagttgatat tgatgaagcc gaggatttta tgattgctga tgcgatttat
      781 aatcatctta ttttacaagg tagttaagac agggggaagg ttaggatgaa caatgtacag
      841 attttagatt gcacacttag agatgggggc tattgtaaca attgggaatt tggatttgat
      901 aatacaaaaa aaattatttc tggacttata gaatcaggaa ttgagattat agaatgtggg
      961 tttctcacaa ataaaacagt atacaataaa aatgttacaa aatatacgac cttagaagaa
     1021 ttagcacaga taattcctga aaaaagagaa ggaaaattat ttgtagcaat gatgaactat
     1081 ggcgaatata atatagagga tcttccagat tatgatggtt catctattga tggaattagg
     1141 attgcgtttc ataaaaaaaa ttgcaatgaa gcgttgaaat tatgtcgtga gataaagaaa
     1201 aaaggctact tagtttttgt tcaagctatg gtatctctca attatacaga tgaagagttt
     1261 ttagcagtaa taaaaaatgt gaatcagttt aaaccatacg ctttttatat tgttgatagt
     1321 tttggaatga tgaaaggaaa agatttaact cgtctttttt acatgattga gcataatcta
     1381 gaaaaagata tacagatagg ttttcactca cataataaca tgcagcttgc atattctaat
     1441 gcacaaaaac ttataaccgt acaaacaaat cgaagcctga ttattgattc ttcggtgtac
     1501 ggaatgggaa gaggagcagg taatcttaac aca
//
LOCUS       DARBFD010000205         1509 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000205 DARBFD010000000
VERSION     DARBFD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.214"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            101..868
                     /locus_tag="SCK08_002887"
     CDS             101..868
                     /locus_tag="SCK08_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1202704.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="SCK08_002888"
     CDS             1060..1458
                     /locus_tag="SCK08_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEG1202705.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DARBFD010000206         1486 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000206 DARBFD010000000
VERSION     DARBFD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.216"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            286..693
                     /locus_tag="SCK08_002889"
     CDS             286..693
                     /locus_tag="SCK08_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202706.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="SCK08_002890"
     CDS             707..1108
                     /locus_tag="SCK08_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202707.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..1481
                     /locus_tag="SCK08_002891"
     CDS             1110..1481
                     /locus_tag="SCK08_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202708.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          571 a          183 c          259 g          473 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aactcaccta atgaat
//
LOCUS       DARBFD010000207         1484 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000207 DARBFD010000000
VERSION     DARBFD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.217"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            170..703
                     /locus_tag="SCK08_002892"
     CDS             170..703
                     /locus_tag="SCK08_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202709.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            715..918
                     /locus_tag="SCK08_002893"
     CDS             715..918
                     /locus_tag="SCK08_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202710.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            1240..>1484
                     /locus_tag="SCK08_002894"
     CDS             1240..>1484
                     /locus_tag="SCK08_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEG1202711.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAG"
BASE COUNT          484 a          231 c          317 g          452 t
ORIGIN      
        1 acaagaattt aatataacat attttcctaa tgcattctaa ctataccaca ttatcaaaaa
       61 tataaattat ctttactaaa taaaatattg acaagcctat accttttgtt ttatgatttt
      121 cacaaagaaa acgttttcta tatatttttc tgtatcagga ggaaattcta tgaaaaaatt
      181 tactttaggg gtagctttgt taactattgg ggcagttgtt ttaccgtcaa ctactatagt
      241 ttatgcttca gaaaattccc aagtggtatc attaaacgaa aacaatgcta ctgaaaatgt
      301 agttattgag tacactgaag atggtatggt cgtaactaaa tatgaggatg gttatagtgc
      361 ggaagatttt gcaaattctt cagatatcat gacaagagct gcagttgcaa gatggggcaa
      421 aaaggaatat acaaatattg ctgtaaccac tggagtagct gctaatgcta ttaatgctgc
      481 cttttgggca ggagggaaag ctagcacttc tatgtttggt atcccaggtt gggcaattgg
      541 tgctttgtta acaggtgcaa gttggactaa aaaaggtgct tctccaggga atgcagttgc
      601 aaaagaatgg gacaaaaatg gtaatggctg gattggattt tattaccaaa ggggatatga
      661 tgcagctgga agagttgtag ctactagata ttcaacgtta taagttaagg agtgatgttt
      721 ttgatatcta aatcattaaa atcattaaaa acatttacta aaaagaatcc gcaaatagct
      781 actattcgta tttttatatt tgctatagct gttgctataa ttgttaatat gctaagcgga
      841 gggcaattat acaaatctgg tttattttta gtgttaatct tagttgtctt agacataata
      901 aagtctaaaa aaaaataaat cgatacattt tagaagaata gggttgtttg aatattacaa
      961 agcaacaatt tcaccttcgg tgaaataagc gaaactttac aaaaagtggc tagcaaatgc
     1021 tagccttttt ttgtattgtg gagcgtggat tttgaggggg attcaagggg gagcttgcgc
     1081 tcctttgaca agcggttatt tgcaccaaat acggaggtat ttggctagca aatatagagc
     1141 ttgccaaacc cttttagtga gctttactca ttgtaccttt ttaggtacaa tggtattgcg
     1201 aacgatacgt ccgtaggacg tactgaaagg gtgggactta tgagcgaaca acatcaaaat
     1261 ttggctagcg atcattctaa aaaatatacc tatcgatccg agccaaaaca aatcagtttt
     1321 cgagtgagcg aatccgaatt tgcaaagctg aagcagtcag ctgaagcttt gcaaatgagt
     1381 gtgccggctt tcgtgaaagc caaggcacaa aacgcgcgtc tggtgacacc gaaagtggcg
     1441 ccagacattg cccaggcaat cgcgcgcgat ttagccaaag ctgg
//
LOCUS       DARBFD010000208         1454 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000208 DARBFD010000000
VERSION     DARBFD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.218"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..625)
                     /locus_tag="SCK08_002895"
     CDS             complement(<1..625)
                     /locus_tag="SCK08_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1202712.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="SCK08_002896"
     CDS             complement(784..1050)
                     /locus_tag="SCK08_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1202713.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="SCK08_002897"
     CDS             complement(1040..1396)
                     /locus_tag="SCK08_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1202714.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          588 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aatt
//
LOCUS       DARBFD010000209         1440 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000209 DARBFD010000000
VERSION     DARBFD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.219"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            105..1406
                     /locus_tag="SCK08_002898"
     CDS             105..1406
                     /locus_tag="SCK08_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1202715.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DARBFD010000210         1424 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000210 DARBFD010000000
VERSION     DARBFD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.220"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(150..1337)
                     /locus_tag="SCK08_002899"
     CDS             complement(150..1337)
                     /locus_tag="SCK08_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEG1202716.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DARBFD010000211         1394 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000211 DARBFD010000000
VERSION     DARBFD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.221"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            169..393
                     /locus_tag="SCK08_002900"
     CDS             169..393
                     /locus_tag="SCK08_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEG1202717.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            413..700
                     /locus_tag="SCK08_002901"
     CDS             413..700
                     /locus_tag="SCK08_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1202718.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            1150..>1394
                     /locus_tag="SCK08_002902"
     CDS             1150..>1394
                     /locus_tag="SCK08_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEG1202719.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAG"
BASE COUNT          499 a          188 c          291 g          416 t
ORIGIN      
        1 actagctgtt taaataactg atcttataat tttatttaaa cgattatgaa ttcaaaacta
       61 ctttttatga cgttaatcat tgttatataa taaaatgtta tataataaaa ttactaagat
      121 tatttaatat aacaagcaat tttaaaaaaa cgaaaaggaa aaacaatcat gaaaaagaaa
      181 gtattaaaac attgtgttat tctaggaata ttaggaactt gtctagctgg catcggtaca
      241 ggaataaaag ttgatgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa
      301 tgttgggtag attggaatca agctaaagga gaaattggaa aaattattgt taatggttgg
      361 gttaatcatg gtccatgggc acctagaagg tagtggtagg gaggttatta taatggattt
      421 tactaaagaa gaaaaacttt taaatgcaat tagtaaagta tacaatgaag caactataga
      481 tgactatcct gacttaaaag aaaagctctt tctttattct aaagaaatca gtgagggaaa
      541 aagtgttggt gaagttagta tgaaattaag tagttttctt ggaagatata ttttaaaaca
      601 taaatttgga ttacctaaat ctttaataga attacaagaa attgttagta aggaatctca
      661 agtatataga ggatgggctt ctattggtat ttggagttaa ttctttggaa taaaatgaaa
      721 atgggactga agaatcaatt ctgattcatc agtcccataa attatagagc cgaaaaatta
      781 tcatatttat agatttatta atagtaaatg gcataagaat ctttgaacaa cgaaaaaaat
      841 gtagctctac ctaattattg atttgcaaca atttcaccga aggtgaaaca agcgaaactt
      901 tacaaaaagt ggctagcatt tgctagtctt ttttgtattg tggagagtgg gggggagggg
      961 gattcaaggg ggagcttgcg cccctttgac aagcggttat ttgcaccaaa tacgtaggta
     1021 tttggctagc aaatatagag cttgccaagc cctttagtga gctttagtga gctttactta
     1081 ttgtaccttt ttaggtacaa tggtattgcg aacgatacgt ccgtaggacg tactgaaagg
     1141 gtgggactta tgagcgaaca aaatcaatat ttggctagca atcattctaa aaaatatacc
     1201 tatcgatccg agccaaaaca aatcagtttt cgagtgagcg aatccgaatt tgcaaagctg
     1261 aagcagtcag ctgaagcttt gcaaatgagt gtgccggctt tcgtgaaagc caaggcacaa
     1321 aacgcgcgtc tggtgacacc gaaagtggcg ccagacattg cccaggcaat cgcgcgcgat
     1381 ttagccaaag ctgg
//
LOCUS       DARBFD010000212         1374 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000212 DARBFD010000000
VERSION     DARBFD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.223"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            33..1372
                     /locus_tag="SCK08_002903"
     CDS             join(33..474,474..1372)
                     /locus_tag="SCK08_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEG1202720.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYRVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          577 a          177 c          239 g          381 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattacaggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DARBFD010000213         1368 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000213 DARBFD010000000
VERSION     DARBFD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.224"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(149..448)
                     /locus_tag="SCK08_002904"
     CDS             complement(149..448)
                     /locus_tag="SCK08_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1202721.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            799..>1368
                     /locus_tag="SCK08_002905"
     CDS             799..>1368
                     /locus_tag="SCK08_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1202722.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNC"
BASE COUNT          464 a          218 c          192 g          494 t
ORIGIN      
        1 tgttaattaa aggataaaat acaatataaa gaaacatctg tttctttatt tagaataaaa
       61 aaatacagac tctgttcaaa taaatgaacc tagcctgcat tatgagattg actatacttt
      121 ttaattcatt gagatgaata actcgttttt ataaatgccc aaaagtaatc ggtgccgaag
      181 ccattcctcg acctaattct ttttcaaatt ttcctattgt tgatatctcc atataaaacg
      241 gtgatactgg tttagacaat tctcccctga ttgctaacgg acgtaaacta cgttccaaat
      301 tatttatgac tttaggataa tactgacctt tttctaagcc tgtctttgcg tctatcaaaa
      361 gtatacgttc cttttctttg atctctggat ttaaaacgag atcatataat tttagtacca
      421 tgttttctat ttcttcactt ttagacatca cgatttcctc ccaaaaatat atttcttccc
      481 actacattct ataaacaaag tatacgctac atctagattt ttttaaatca ttactggatt
      541 gttaaaacct ttcaatacac tgttatggca acgattttta ttttaaaaac attctaaatt
      601 aaatcagtaa aaatttttat tctaagattt ttattagaat tacgcctaat ttgtgtctct
      661 cagggcaatt ctaagaacgt ttcaagagat agcgacacta tcgttatcga aaaaggcgaa
      721 aacctatctc attttctgct aatgtaagtt ggtttagctt ttgttaatat gactttatga
      781 aaatggaggt aattaaatat gatttttcct aaagttaatg aagcaagctt gaataaagga
      841 tccttttttc atatctatga aaacgaagat tttgaactgt ataatatcct ttcacaaaaa
      901 gaggtcatcg aaaaccaaat aatctgccat ccaattattt acaaatctat tttcaagtat
      961 gcattttttg cttccaatga ttttcaaaga atggagatta ccgactgtat attcgaaaac
     1021 tgtgattttt caggctgtat attgacaaaa agcatgttgc atcgcgtgaa atttgtcaat
     1081 tgtaaattaa caggtactaa attgatggaa agttatattg ggaacacgtt attcgagaat
     1141 tgtaaaatgg actatgtaaa tttatctggt tcaaacataa aagaaagtac ctttgagcac
     1201 tctgtcttat caagcgcaga ttttgtagac tgttccctca ctaaaataat gttttctact
     1261 aatgatttag aaaatgctag cttttttgat actgatttaa aaaatatcga tttaagtagc
     1321 aactcctttg aaatgattga agtacgctcg gaacatctaa aaaattgt
//
LOCUS       DARBFD010000214         1359 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000214 DARBFD010000000
VERSION     DARBFD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.225"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..338)
                     /locus_tag="SCK08_002906"
     CDS             complement(<1..338)
                     /locus_tag="SCK08_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202723.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNP"
     gene            complement(340..>1359)
                     /locus_tag="SCK08_002907"
     CDS             complement(340..>1359)
                     /locus_tag="SCK08_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEG1202724.1"
                     /translation="LAALISIYQFIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPN
                     VGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLV
                     LFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRK
                     VEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDF
                     YGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFS
                     LLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLY
                     LKKEN"
BASE COUNT          524 a          223 c          170 g          442 t
ORIGIN      
        1 ggattttttt tgtaaacatt ggagaaaagt atttttgtga aataattttc cccctgtata
       61 aaaatttcat cataaacgga taaatcatcc gagaaaaatt gattatggtt agttaacaag
      121 ttaattttac gtttaaagga agaaccgatt ttccggtgat tcacaaaaac aacatcatta
      181 aatagcccta attcttttat acgctcacta tatcttctag cgttttcaaa ttcagaataa
      241 ataaataaat ctccttttga ttctcggtaa aaattttgat aaacatttaa tatattaatc
      301 aaatgtaaag gagtccaagc aatgaaagct gtcttcatat taattctcct tttttaaata
      361 caagtaagaa atgcttccca aataatacca gaaaacaatt gaaatgacat cgcgcttatt
      421 gaaaagtaca tgagcctcca ccatttcatc tataaaaata gatagtataa aaattaaaac
      481 aataccaaga aacgcataat tcactttact tctaaaaaat gttttgagta aactaaagat
      541 atttaacaca tagaaagcat agatacatat aaaagcaatt ataccacttg atacaagtac
      601 ttgaagaaat ccattatgca tgtttccatg tgctcttttt agttcattca catctgtagt
      661 atcaagatct acttttttca aaaattttgc atcgctattc ccatagaaat cggtctctcc
      721 ataaccaaat aacaaagttg gtcttataac tttaagcgca gctttccaaa tcgtaaaacg
      781 accagctgaa atttcggagt tatcactaga atgctgtaat attacttctt tttctggaag
      841 tggagtttta tctgattcat ttgactctat aacttctact tttctatcaa tcgatacagg
      901 aattgttgct aaaaaatcct tagcataatt agtaccaaaa gaaaaatata ccaatagaac
      961 taataatatg cttaccatcc atttgatttt tacaggtagt acttctctaa tgctctttct
     1021 aaattttgaa ttggcaaaca acaaaagaaa taatactaaa aaagaacaaa ttactacctg
     1081 ggcacctcta gacgaagaaa gtgtaatata aaatatttgt acgataaaat tcatcatata
     1141 aaaaacacga atcgtttttg ttatttttga aattaataca ctaattaaac ttaaaataat
     1201 agaagtatat ccaaacatag ctccaacatt tggacttgta tagacaccaa ataaccgatg
     1261 ttcaataaaa ccttgtcttg caagtaatcc tggcgttcct gttgggacat gataagaaat
     1321 caaaaaaata aattgataga tagatattag cgcagctaa
//
LOCUS       DARBFD010000215         1355 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000215 DARBFD010000000
VERSION     DARBFD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.226"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1355
                     /locus_tag="SCK08_002908"
     rRNA            <1..1355
                     /locus_tag="SCK08_002908"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DARBFD010000216         1287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000216 DARBFD010000000
VERSION     DARBFD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.228"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..848
                     /locus_tag="SCK08_002909"
     CDS             <1..848
                     /locus_tag="SCK08_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1202725.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DARBFD010000217         1267 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000217 DARBFD010000000
VERSION     DARBFD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.229"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..833)
                     /locus_tag="SCK08_002910"
     CDS             complement(<1..833)
                     /locus_tag="SCK08_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEG1202726.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     D"
     gene            complement(839..>1267)
                     /locus_tag="SCK08_002911"
     CDS             complement(839..>1267)
                     /locus_tag="SCK08_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1202727.1"
                     /translation="LIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVL
                     MLPSLFEGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANV
                     DISHRDSERSILIKADYNIQTAYKILESKYYELFELIQRG"
BASE COUNT          390 a          238 c          145 g          494 t
ORIGIN      
        1 ttatcaaatg tctgtgctat ttcatgattt attctctgtg ctaaaacaat catataatct
       61 atcatcaaat aatccaccat catatcatta tttttccaat aatttaaaaa aaagtctcta
      121 attatagcaa acatccatct agactcttga ttacatgcta atgaataacc cgcaaaatat
      181 cctgatgcta cggatgcgtg agcataatca ggcctcctaa tagaccaaat atcgatatca
      241 aaatactcat ctggtattgg ttttgtacaa taaaatgtgg cgtctaacca caaacctcca
      301 tgttttgcta gcaaagataa tcttaataaa tcagaataat ttgttctcgt aatgattcct
      361 ttgtttttct tttcttccac ccattttggt atatcaacat attgtctata attatcttct
      421 gttaaaatta taacagtatg tttccctgca tttcgtttta ttgattttac acaagctttt
      481 acaatttccg gcgattgatc caatccctgc caccaacaaa cccaaatgca gttagaatat
      541 ggtgattttg attgattaat tatttgattt tgactataat catatgtttt caaaaaatct
      601 gaaaaagtct tttcataata cttcagcatt acctcatgtt tttttaataa atgttccttc
      661 tgttttttac tttcattata tccattacga ttcatgactt gaatatcaat tttagcttgt
      721 aacgtggcaa atgcttcctt ccaagatgtt atctttgcaa ttcgtcttgt tgcattcaat
      781 tcttcggata tgcgatgtat aattcttttt atacttgaat gtttctgtgt catattcttt
      841 aacctctttg tattaattca aataactcat aatattttga ttctaagatt ttgtatgctg
      901 tttgaatatt ataatctgcc tttattaaaa tcgaacgttc tgaatctcga tgagagatat
      961 ctacatttgc aatctctctc gcccattctt ttgccgattg atttaaactt atgaaattgg
     1021 tcttatcact gaaagcaact tctgtaggaa ctttctccga aaagaaacac ggtaaatctg
     1081 caaattgagc ttcaataccc actaacggaa ccccttcaaa taatgatggc aacattaata
     1141 cgtcaaatgc ttgataaaat ttttctatat catttctatt tcctaaaaat ttaatatcat
     1201 catctatgcc acttttcttt gcataatttt ttatttcttc ttccatatca ccagttccaa
     1261 taattaa
//
LOCUS       DARBFD010000218         1265 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000218 DARBFD010000000
VERSION     DARBFD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.230"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..688
                     /locus_tag="SCK08_002912"
     CDS             <1..688
                     /locus_tag="SCK08_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202728.1"
                     /translation="KKSIFSTSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYDVK
                     NIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNS
                     IFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSP
                     SMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAE
                     LRKLVEYGII"
     gene            675..>1265
                     /locus_tag="SCK08_002913"
     CDS             675..>1265
                     /locus_tag="SCK08_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG1202729.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVI"
BASE COUNT          438 a          158 c          186 g          483 t
ORIGIN      
        1 gaagaagagt atattttcaa caagtaaaaa cgttcaaaga gtttttaaaa tattaaataa
       61 aaatagcatc ttcaaaaaaa aaattgacga ttattttgtc tattgtccgg aattgttact
      121 ttatgatgta aaaaatataa aaaaaattcc caaaatagat gaaaacagta aattgatgaa
      181 aaacatacaa tcaatctttg ggatagaaga agtttttgtc ggagataaat gtatcttttt
      241 tgatcaacct ttattatctg atggttatgg catagatgaa acagatattt tcgctaaagt
      301 aaatagtata ttttctggaa aaaaagaaac tttaattaag cttcatccta gagcaaggac
      361 aaatcgttat ggtgaaaaaa acatattgaa ttccagttta ccgtttgaag tcttgctact
      421 tgaaaatgac ttctcttcaa cacttttgat tagtcctata tctacaattt ctttttcacc
      481 atctatgatg tttgaaaaga cgatcaactc tattatgttg gctaaattaa ttttaaatga
      541 atatacagca ttatctgaag ataaaaaaga aacattgaca aatattgttc ttttctgtga
      601 acattataac cgtctatcag aagaaaaaat tttactccca gaaaactggg cagaattaag
      661 aaagttggta gagtatggga ttatttaaca cactttccaa gtcaaaatta gttaagtcca
      721 gtttctggta tactataggg aattttttta ttaagggaat ttcattcata actattccta
      781 tttttgtaaa tattactact caagcagaat atggtttgat taataatttc acttcatttg
      841 tatcaatttt ttcgattctt gttgggctaa gcctaaattc ggcaattaat aatgcaagtt
      901 ttgactataa agatgacata aatgaattta tgtcatcggt actttctcta gctacacttt
      961 cgttaattgt gttcttatta atagggaaca tatatttttt gttttcaaat gctttttttg
     1021 atatgaacca acttgtattt aatttgatgt tattacaaag ttttgctagt tttgtaatta
     1081 gttatatcca gtcttatttt acgattaact ttaaccattt taaatatttg attttatctt
     1141 ttatatctac ttttttgaat attgcaatct ctgtagcttt gatgcagaca ttatattttg
     1201 atgataaata tgttggcaga gtagtcggcg gaacactgtc atttgtgttg ataggtttag
     1261 taatt
//
LOCUS       DARBFD010000219         1263 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000219 DARBFD010000000
VERSION     DARBFD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.231"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..434)
                     /locus_tag="SCK08_002914"
     CDS             complement(<1..434)
                     /locus_tag="SCK08_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202730.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(451..>1263)
                     /locus_tag="SCK08_002915"
     CDS             complement(451..>1263)
                     /locus_tag="SCK08_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG1202731.1"
                     /translation="ILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYD
                     PEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKL
                     AQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLS
                     PLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTA
                     KIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          432 a          217 c          137 g          477 t
ORIGIN      
        1 taattgcttt cactcaaata cttataatct attaacttgt agttgtttaa cttttctgaa
       61 aaagttagac ccacgtaata atcttcccag ctttctcttt caattaactg atccaatttt
      121 tgataatcca taaattcatc atgtatcaaa aagaataaat aacttgatat tttagcatct
      181 agcttaatca gattaaataa tgtttgttta aaattgctac cattaatatg actgatagat
      241 tctctagctt cttcacataa actttttact ttattaatta ctactttgtc atattcattt
      301 aacacactat tattaccatt acttagacgc tcagattcta cgaaacaact caaatcagaa
      361 atttcccaaa aaaatttttt gttaaaatcc aatttcctca actcctccaa actacgtaaa
      421 tgatatagtc tcataataaa ataaaacctc tcagctaatg ttgttgaaat tgtccactaa
      481 acctaattga tattgcttag gagatacacc tgcgtacttt ttgaatactc tcgaaaagtg
      541 tgaagaatct gaaaatccta aacctaaaga aacttcactg atagtatggt ttgatttcaa
      601 cattatttta gccgtagaaa tttttcgctt agagatataa ttataaatcg taatatgcat
      661 ttcattctta aagctttctc ttaatttagt ttcacttaca gaaaaataat tagctatttc
      721 ctttacagta attcttttat agatatttaa gccgatgaat tgaacgacag aagaaattaa
      781 gggagaaaga tgctctatct tagtttttcc gatttcctta gtataataaa caatcccaga
      841 atccctcact tttaagacct caggtaacga tacagctaat tcgttttctt gtatcaaagc
      901 atctctactt tgatacgctt ctattatatc cattcctaat gttatagacg tttgtgctaa
      961 cttttcaaaa acaatgatag aataattttt ttcagaccga atcgtatctc cactaacact
     1021 tggaattatt ccattactca gattaaaaac catgttttct aaatttcgaa tatctccatt
     1081 tttaacatat tccagtattt tattttcata aagaaacaga tatatttctg gatcgtaatt
     1141 ttgtgataac agattatcta ttttgatttg ctcgatagtt ttacttagat tttctgtata
     1201 ttcaattaac ggttcatgga atagatcttc tatttttcca gtaaaaaaat aatgaactaa
     1261 aat
//
LOCUS       DARBFD010000220         1246 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000220 DARBFD010000000
VERSION     DARBFD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.232"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            200..742
                     /locus_tag="SCK08_002916"
     CDS             200..742
                     /locus_tag="SCK08_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202732.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="SCK08_002917"
     CDS             898..>1246
                     /locus_tag="SCK08_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEG1202733.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DARBFD010000221         1213 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000221 DARBFD010000000
VERSION     DARBFD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.233"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            158..1213
                     /locus_tag="SCK08_002918"
     CDS             158..1213
                     /locus_tag="SCK08_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1202734.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT          497 a          112 c          236 g          368 t
ORIGIN      
        1 atatgatgaa aaagtatgga acttattaaa gaaagatact ttactgttta agttgacatg
       61 gaaacaatct tttaaaaagc aaaaagatga taaagttact ttttatggga tgttggtaga
      121 aaacaaatta ttataaatat tagaggtata aaaagttatg aaacacaagg taagtgtgat
      181 agtaccaatt tacaatgttg aaaaatattt aagaaaatgt gtggatagtt tattgagtca
      241 gacattaaaa gagatagaaa tcgtattagt tgatgatggt tcgccagatg cttcggggga
      301 aatagcagat gagtatcaaa aaaaatattc taatgtaaaa acaatacata gagaaaatgg
      361 ggggttagga ccggcaagaa atacaggaat tgagaatgcg acaggagaat atgtggcttt
      421 tcttgattca gatgattggg tagaaagtga tatgtatgaa aaattatact tagttgcgtc
      481 caaagaaaat gctgatatag tagttagtgg acattgtgat tttgcgaatg acaaagctat
      541 ggtgaaaaag caacatcctt tgagtgggaa tgtatatgat acgcctaata gtattaaaaa
      601 tgtacgaaaa aatttatttg gacatagtcc aaaagatcaa gaagtagagg ccttcccgat
      661 gtcagtatgt atgtctttgt ataataaaaa tctgctagat aaatatagac taagatttga
      721 aaaaatttta tctgaagata caatttttaa cttgaatgca tatgatgtcg caaataaaat
      781 tgcttttaca gaatatacag attattgtta tagaaaagaa gaacaagagt ctattacaaa
      841 aagtttatct ccaaataagc agacggaatt taaagaattt ttaataactc taaaagctat
      901 agcatcgaaa gaagatttag aatgtcaaat cagagcaaaa cgtatggcaa ttgattattg
      961 taggctttat gtgggcttag ttgatgatgc aaatttggga attaaagaga aaagaaagta
     1021 tataaaaaaa tttattgaag atgagggatt aattcaatgt tggaagggat atcctacaaa
     1081 tacgctacca tttcaacaaa aattttttca taaaatgctt gaatgtaagt gttatacaat
     1141 attgttgttg ttaagtagaa ttagaaagat aattaaaaat atgaccagaa aaaaattgtt
     1201 tataatatta taa
//
LOCUS       DARBFD010000222         1207 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000222 DARBFD010000000
VERSION     DARBFD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.234"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1207
                     /locus_tag="SCK08_002919"
     CDS             <1..>1207
                     /locus_tag="SCK08_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEG1202735.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMR
                     IRIFLINQL"
BASE COUNT          400 a          234 c          232 g          341 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaatc
     1201 aattaat
//
LOCUS       DARBFD010000223         1199 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000223 DARBFD010000000
VERSION     DARBFD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.235"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(305..661)
                     /locus_tag="SCK08_002920"
     CDS             complement(305..661)
                     /locus_tag="SCK08_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEG1202736.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /gene="spx"
                     /locus_tag="SCK08_002921"
     CDS             complement(675..1085)
                     /gene="spx"
                     /locus_tag="SCK08_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1202737.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          386 a          222 c          168 g          423 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaa
//
LOCUS       DARBFD010000224         1141 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000224 DARBFD010000000
VERSION     DARBFD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.237"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..579
                     /locus_tag="SCK08_002922"
     CDS             127..579
                     /locus_tag="SCK08_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202738.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..>1141
                     /locus_tag="SCK08_002923"
     CDS             593..>1141
                     /locus_tag="SCK08_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202739.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSG"
BASE COUNT          406 a          152 c          229 g          354 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 t
//
LOCUS       DARBFD010000225         1129 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000225 DARBFD010000000
VERSION     DARBFD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.238"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(17..922)
                     /gene="xerC"
                     /locus_tag="SCK08_002924"
     CDS             complement(17..922)
                     /gene="xerC"
                     /locus_tag="SCK08_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEG1202740.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          335 a          230 c          194 g          370 t
ORIGIN      
        1 gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt tgtaaacttt
       61 cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct agcaattctt
      121 ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta tggcgcagca
      181 tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc aagacatatt
      241 caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata tcatgctctt
      301 cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc agagcatctt
      361 gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg atcaacatga
      421 cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt aaacggatgc
      481 ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa atccaaagga ctatctcctg
      541 ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc agtctcaaat
      601 ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct tctttgagaa
      661 gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt ctgctatagc
      721 taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat tccgccttca
      781 gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg tcttcttcat
      841 atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc aaaaattttt
      901 gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa taatttagca
      961 cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct aagtttgttc
     1021 acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac gcaagaaaaa
     1081 gggctctata atttatggga ctgatgaatc agaattgatt cttcagccc
//
LOCUS       DARBFD010000226         1116 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000226 DARBFD010000000
VERSION     DARBFD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.239"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="SCK08_002925"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="SCK08_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEG1202741.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DARBFD010000227         1106 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000227 DARBFD010000000
VERSION     DARBFD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.240"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1087
                     /locus_tag="SCK08_002926"
     CDS             <1..1087
                     /locus_tag="SCK08_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1202742.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DARBFD010000228         1105 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000228 DARBFD010000000
VERSION     DARBFD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.241"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            119..>1105
                     /locus_tag="SCK08_002927"
     CDS             119..>1105
                     /locus_tag="SCK08_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202743.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDIL"
BASE COUNT          360 a          118 c          193 g          434 t
ORIGIN      
        1 aaacttgtat ggaaaaatca gtacaatacc attatttaat gttcttcact taaagcgcaa
       61 aaaatatcag tttattaaac tcgtatatag aacaaatata tgtagacggg gcgaaacgat
      121 gagtgtaaaa aaaatgctat ttatggtaag tacaataatt gtattaacat cccgattttg
      181 gggaattaat actagtttat ctatgcgata ttttattatg gcaatatggg atttatattt
      241 tatgttaagc tatttttctt atcgtgagaa aacatataaa aaatacgtta tcaagaataa
      301 cttttggttg actataatgc cgtttgtgat attttctatc tatactctaa taatatgggg
      361 aattggttca aatgcagaat ttgaaaattt tataaagtta tgttccacgt tgctttattt
      421 aattcttgca tatggttacg catgtacagc attttactta tttaaggcag aaggaattga
      481 ttttattttt tggtcaggtg ttatttcata tatgagtggt tcagtttttt atttaattgt
      541 atcttatggt ttaaatggtt taatttctta tataaaaagt cttataacag gtgaaacaaa
      601 tactgcaaat tttgctatgg aggtacatga tttaactttt gcgatggggg tttttttcct
      661 ttattacgta ttttttgaaa ataaaaatac aaaaaaacat aagataaaaa ttatcgtatc
      721 tttaatttta attttcatgg gattgaagag aattgaactg ttagctttaa gtggtgcaat
      781 tctagtgtat tatgttttat taaagtgggg aaaaaagatt caaatccgtg cattagtgtg
      841 tactatttgt gcaactgtta tttcgttagt gtttatttac attatagata caggaatttt
      901 ggcattgctt atgtcaaatt tgggcattac agatgacggt gcgaggcttt cattttatct
      961 atattcatct aaattttatg aacttggttt aggatatgta ggaacaggat ggacatggtt
     1021 ttcaaaatac ttttttaatc tttatttatc gcactttagg ttaaatggtg gaagaattgc
     1081 agcaagccta cacagtgata tatta
//
LOCUS       DARBFD010000229         1069 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000229 DARBFD010000000
VERSION     DARBFD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.242"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..969
                     /locus_tag="SCK08_002928"
     CDS             <1..969
                     /locus_tag="SCK08_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1202744.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DARBFD010000230         1068 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000230 DARBFD010000000
VERSION     DARBFD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.243"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1068
                     /locus_tag="SCK08_002929"
     CDS             <1..>1068
                     /locus_tag="SCK08_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEG1202745.1"
                     /translation="VSYETLQKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKN
                     GKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIAS
                     LLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIED
                     KMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVL
                     PNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRANIN
                     HYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFS
                     LLAELPETLDDSFREKLQNLLT"
BASE COUNT          338 a          217 c          211 g          302 t
ORIGIN      
        1 ggtctcttac gagacccttc agaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc
       61 tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat
      121 tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt gaacaataca atcatatgcc
      181 tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgca cccattggac
      241 ggctcaaagt tattttgtcc aacccagaca ttcaatcgca aatcatgtta gatataaaat
      301 tgcttcttta ctgactgaaa aagtatcttt atcttttatt gcgaaaagct gtcaggtatc
      361 tttgaccacc gttattcgta cattgaaaga gtttaaaagc tatttaccaa agcaatctaa
      421 gaagatcctc cccagagtat tgatggttga cgaatttcgt tcgcatgctt ccatagaaga
      481 taagatgagc tttatttgcg cggatggcga aacaggaaaa ttaatagatg ttttgcctac
      541 gcgtaaatta cctcgattaa caagctattt cttaggttgt accaatccag aagaagtaga
      601 attcttggtg acagacatga acgccgccta cttccagctc accaaacgtg ttctgccaaa
      661 tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac atgaatcaag cgttcaatga
      721 gttgcgtatc cgtgaaatga atgaacttcg taaagccgga cagaaaagcc aggcagaaaa
      781 actgaaaaag aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata
      841 caaaacatgg aaaagtttcc gagcaccaaa atacccattt cttactgaag caatgatgat
      901 tgatcgattg cttgagtttt ctccgcccct aaaggaggcg tatccctttt ttcatgaatt
      961 agttgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg cagaacttcc
     1021 cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac cttctgac
//
LOCUS       DARBFD010000231         1063 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000231 DARBFD010000000
VERSION     DARBFD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.244"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..153
                     /locus_tag="SCK08_002930"
     CDS             <1..153
                     /locus_tag="SCK08_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1202746.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="SCK08_002931"
     CDS             159..575
                     /locus_tag="SCK08_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202747.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="SCK08_002932"
     CDS             681..944
                     /locus_tag="SCK08_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1202748.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1063
                     /locus_tag="SCK08_002933"
     CDS             944..>1063
                     /locus_tag="SCK08_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1202749.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPY"
BASE COUNT          407 a          132 c          215 g          309 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tat
//
LOCUS       DARBFD010000232         1063 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000232 DARBFD010000000
VERSION     DARBFD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.245"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(38..994)
                     /locus_tag="SCK08_002934"
     CDS             complement(38..994)
                     /locus_tag="SCK08_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202750.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caa
//
LOCUS       DARBFD010000233         1062 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000233 DARBFD010000000
VERSION     DARBFD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.246"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..963
                     /locus_tag="SCK08_002935"
     CDS             4..963
                     /locus_tag="SCK08_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEG1202751.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          369 a          200 c          210 g          283 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc ca
//
LOCUS       DARBFD010000234         1061 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000234 DARBFD010000000
VERSION     DARBFD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.247"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..995
                     /locus_tag="SCK08_002936"
     CDS             <1..995
                     /locus_tag="SCK08_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202752.1"
                     /translation="IISDHSKNTKEIAKKIEEKKVFDGCHYLESKSFDDNIGYFQKIK
                     DCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACLYRNNKNVQVSILE
                     EGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFPNIYTGRENTIQIPLV
                     TAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYELVCKIADLVGKDN
                     LIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSGSVISGSTMS
                     AKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIAESLDDIL"
BASE COUNT          417 a          119 c          183 g          342 t
ORIGIN      
        1 ttattatttc tgatcattct aaaaacacta aagaaattgc gaaaaaaata gaagaaaaaa
       61 aagtgtttga tggctgtcat tatttagaaa gcaaatcttt tgatgataat attgggtatt
      121 ttcaaaagat taaggactgt cttcagattt cttgtggtaa atcaaatcga tatgcctatt
      181 atttgaaaaa ctgtaaaagt gaatattttg atgaatttat ttgtttttca atgggagagg
      241 tttggttgga aggaatatat gcttgcttat atcgaaataa taaaaatgta caggtatcaa
      301 ttttagaaga aggtatttta agctatggag ttgacattat tccaacagca agaacaaaag
      361 ctatcgcagt actcaattac ttaaaaaaaa ggactccact atataacaaa agctataatt
      421 tttattgttt ttttcctaat atatatacag gaagagagaa tacaatacaa attccattag
      481 ttacagctgg gtcaagaacc gcattgcaac tttcggatat ttttaaccct atgatagata
      541 tttataaaga aaaaaaatat atatttttta cgtctgttta tgattttgag ggcggagaac
      601 ctgtcggaga atatgaatta gtatgcaaaa tagcagactt agtaggaaaa gataatctaa
      661 ttgttaagac gcatcctaga gatgtaagaa caatttataa agataatgga tttatcgagg
      721 ataaaaattc atcaatacct tgggaggcga ttcaattgtc aggcgatttt aacgataaaa
      781 tatttatgac aattaattca ggaagtgtta tttcaggtag tacaatgtct gcaaaacctg
      841 tacgcacgta ttatatgtat aaattgtgta acatagaagg aaatccgtct tgcaaaaaga
      901 atgcgtatga tattgagcga ttgcttaagc aaaaaacgat gaaaaacatt ttaaagaatg
      961 tgaaaatagc agaaagctta gatgatatac tatgagcaaa actaaatatt taattaaaaa
     1021 tatgggaata ttaacaataa gtaatttttc ttcaaaaatt t
//
LOCUS       DARBFD010000235         1050 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000235 DARBFD010000000
VERSION     DARBFD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.248"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            5..979
                     /gene="bsh"
                     /locus_tag="SCK08_002937"
     CDS             5..979
                     /gene="bsh"
                     /locus_tag="SCK08_002937"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEG1202753.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          360 t
ORIGIN      
        1 aactatgtgt acgtctatta cttatgtaac aagtgatcat tattttggaa ggaattttga
       61 ttatgaaata tcttacaatg aagtagttac tgttactcca agaaattata agttgaattt
      121 tcgaaaggta aatgatttgg atactcatta tgcaatgatt ggtattgccg ctggtatagc
      181 tgactaccct ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa
      241 tttttctggg tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt
      301 tgaatttatt ccttggattt taggacaatg ctcaacagta ggagaagcta aaaaattgtt
      361 aaaaaatatc aatttagcaa atataaatta tagtgacgaa cttcctttat cccctttaca
      421 ttggctatta gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca
      481 tatatatgat aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt
      541 taatttaaac aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca
      601 aataagtttg aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc
      661 ctcagtatct cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc
      721 agagtcagaa agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg
      781 tttgtgtgat gtaggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt
      841 tgacaaagga atctattatt atcgaacata tgaagacagt caaattactg caattgatat
      901 gaataaagaa gacttagata gtcataagtt aattagttat ccaattatag aaaaacaaca
      961 aattaaatat ataaattagt taatgtgttg tgattgatta tttaatatag atataataca
     1021 aaaaggcaaa gatattttca aaacattttt
//
LOCUS       DARBFD010000236         1032 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000236 DARBFD010000000
VERSION     DARBFD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.249"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            50..583
                     /locus_tag="SCK08_002938"
     CDS             50..583
                     /locus_tag="SCK08_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1202754.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            598..768
                     /locus_tag="SCK08_002939"
     CDS             598..768
                     /locus_tag="SCK08_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202755.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
BASE COUNT          435 a          183 c          172 g          242 t
ORIGIN      
        1 tatctgattt agataattta ttccagtagc aacctaggag gcaaccagta tgcaaccaca
       61 agaaaccttt acaataaagg aattgtcaga tctttttaag atgagtaggc aagcaatatc
      121 gaaacatatc caaaaattag attcttccat gatagctaaa aatgagcgtg gttataaggt
      181 tgtattgcgt tctggagtat tgcaacttgc tcgcaactta gacaatcaaa ggttgctaga
      241 taagttgcat aaacatgaac aaaacaacga taaaaatgct tatataccaa acaaaaacgt
      301 ggttgcaaac aagttgctag atcagttgcg tgaagaaaat gaatttttac gagaacaact
      361 tgccctcaaa gagcaaaata attctcatat acaaaaacta ctcgaccagc aacaacaact
      421 caccctgcaa gccaaccaac aaatcgaaaa acttcaggag caactgcagt tgacttatac
      481 ggaagagtct tcagagaata gttcaacgac tctttctgaa gcaacagaaa caatggacga
      541 acaaacaaag aattggaaaa aatggtggca gctatggaaa tgaagaagga aatacttatg
      601 acaaaaaaac acttgctatt cggttctatc ctactaggaa tattaagctt tagcccaatg
      661 ttattatcag tggccaacaa aggaaataaa aaaggggaac taaaactcaa tcctaattac
      721 tacaacagtc aaaatgagga agtatttgta gaaaaacttg ataaataaga aaagactgct
      781 cgtacgagca gtctttctga aagagattat cctattatta accttagaaa agaataataa
      841 atgaatatga gatatgtaaa aagcataaca aacacaaaca aaaataacaa gtatttaaaa
      901 aatgaccgat caatattcac aaagaattag aaagaaacaa ccgcagaaaa ccaagcctta
      961 aaaagagctt ttaacctaga ccaataaaaa ttcacaaaat cagcgcctgg atcccttggt
     1021 acaggcgtta ct
//
LOCUS       DARBFD010000237         1023 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000237 DARBFD010000000
VERSION     DARBFD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.250"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            105..797
                     /locus_tag="SCK08_002940"
     CDS             105..797
                     /locus_tag="SCK08_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1202756.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          461 a          116 c          152 g          294 t
ORIGIN      
        1 aataattttt tagacaaaaa acagaaagtg tctaaagaaa gcttgacgaa gaaaataatt
       61 tttaataact tatttaagga aattttttga aggagaagat aaaagtgaga aaaaagatta
      121 ttattaatgc tggagtaatt ataggattga tgttagtaat tttctccgga aagcaatatg
      181 ctgataatat tttattatgg ggaggggaag ataatattga aaagatcaac aacaatttaa
      241 ctatattaga cacagctcta gaaaataaag agcaaaaaat tagtatttta aattcacaat
      301 tgtcttcaaa caaccaaact cttgaacaac taagaactaa tattgaagtt tccaaacaaa
      361 aagtggcatc tttagaaaac gacaagaatc aattagtatc agagaaaact aatttagaaa
      421 atcaattgtc agcaaaagac tctgaattgc aaggaaaaca aaatgaaatt aatgcgaaaa
      481 tagacgaaat taaccaaaaa gtagctgaaa taaatcgatt aaatagcgat tggaataatc
      541 gtttacaaaa tgaaaagaac aaatttaatg aagaacaatc tactattaat agtttaaacc
      601 agcaaattaa tcaaatgttg agtgaaaaaa ataacattca aaatcaactg caagctactc
      661 agcaaaagct agaagaagaa aagtcaaaaa atagtagttt gaataattac attgaaaagc
      721 ttcaaaaagc taaaaatgat gtagaggata ccgcaaataa agcacaacaa attgtaaatg
      781 aacatacaat ccaatagata tagttttaat ttaacaaatg gtatgtttgg agataatgag
      841 atagaatata aatgtcataa tattatacaa tctaaacacc gtttcctttt ctatttttga
      901 tgacatcagt atatagctac tcgaaagagt agtcttttta ttattctcgc ttattattga
      961 tatgaaaatt tcgatttgac aaaaggtacc gaataatttt gtaatatgaa ttattatcaa
     1021 gaa
//
LOCUS       DARBFD010000238         1013 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000238 DARBFD010000000
VERSION     DARBFD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.251"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            380..1013
                     /locus_tag="SCK08_002941"
                     /pseudo
     CDS             380..1013
                     /locus_tag="SCK08_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          347 a          183 c          186 g          297 t
ORIGIN      
        1 caaaagtagt gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag
       61 aaagaaatga acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga
      121 aaaacaatct taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt
      181 tagcaagcta gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa
      241 atcattatgg atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa
      301 ttcgcaaaat ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc
      361 ccattgttat tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa
      421 gccgtcaaaa aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc
      481 aattgtggat aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat
      541 tttgcctaca cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga
      601 agcggtggaa tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt
      661 cttaccaaat gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc
      721 gttcaatgat tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga
      781 agccgagaaa gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca
      841 ctatgattac aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc
      901 aatgatgatt gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt
      961 ccatgagctg accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DARBFD010000239          994 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000239 DARBFD010000000
VERSION     DARBFD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.252"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..912)
                     /locus_tag="SCK08_002942"
     CDS             complement(25..912)
                     /locus_tag="SCK08_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEG1202757.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          320 t
ORIGIN      
        1 aatttctttg cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata
       61 actggacata gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg
      121 tgcaatatcc catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt
      181 tacatctgtt gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact
      241 tgtggttgtt gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt
      301 aatcaatgag ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta
      361 cgctgttgca tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc
      421 gcccgtggaa aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata
      481 agaagaatat tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt
      541 ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt
      601 tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat
      661 gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc
      721 gataaacgct tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg
      781 cggtaccgct gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat
      841 cgctttttta ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt
      901 cagctcttgc attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt
      961 aaaacaaact taattaatta aattatactt ttaa
//
LOCUS       DARBFD010000240          994 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000240 DARBFD010000000
VERSION     DARBFD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.253"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="SCK08_002943"
     rRNA            complement(<1..>994)
                     /locus_tag="SCK08_002943"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DARBFD010000241          988 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000241 DARBFD010000000
VERSION     DARBFD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.254"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..704
                     /locus_tag="SCK08_002944"
     CDS             <1..704
                     /locus_tag="SCK08_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1202758.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          335 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataa
//
LOCUS       DARBFD010000242          914 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000242 DARBFD010000000
VERSION     DARBFD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.255"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>914
                     /locus_tag="SCK08_002945"
     CDS             <1..>914
                     /locus_tag="SCK08_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEG1202759.1"
                     /translation="FLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFF
                     LLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIA
                     TVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYG
                     DNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVL
                     NCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVI
                     YVVRKRAVGKDIFIKHYHIVVESWGLLFV"
BASE COUNT          284 a          114 c          177 g          339 t
ORIGIN      
        1 tttttgtatt atatatccta tgtatggaat caatttctaa tacaatttgc taaagggtta
       61 gaaaaagtta aagctatggg catagcaggt attatcagca caattgtaac aataattgcc
      121 aatattttct tcttgcttat tctgaaatgg ggattaattg gttttttcat tgcaaatatt
      181 ttatcacaga ttatttcagc tacttatctg tcagtattag tgagaatatg ggaatatatc
      241 gattttgagg ctaaagatcc tcagctaaag agagaaatgc taaattactc gattccatta
      301 attgcaactg ttgttggatg gtggattaat agtacagctg ataggtatgt ggtttccttt
      361 atagtaggtg tctcggcgaa tggcttattg tctgtggcat ataaaatacc tcaaataatt
      421 aatactttgc agggaatatt tatacaggcc tggcaaattt ctgcaattaa agaatatgga
      481 gataatgaga ctgcaaaatt ttatggaaat acatttgtta tgattaattt gttaatgtgc
      541 ataacctgtt caagtcttat catattaact agaccgtttt cacatattct ttatgcgaaa
      601 gacttttatg aagcgtggaa atatgttcca tttttattaa tagctaatgt tttaaattgt
      661 gcatcaggtc tgttaggacc tatcttatca gcaaaaaaag attctaaatc catgatgtgg
      721 tctgcaatca taggggcatt agttaatgtg attatgagtg tagtccttgt ttataagata
      781 ggggttcaag gtgccacaat agccactgtt atatgttcat atgtaatata tgttgttcgt
      841 aaacgagctg tgggaaaaga tatttttata aagcattatc atattgttgt ggaatcttgg
      901 ggattattgt ttgt
//
LOCUS       DARBFD010000243          904 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000243 DARBFD010000000
VERSION     DARBFD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.256"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            265..888
                     /locus_tag="SCK08_002946"
     CDS             265..888
                     /locus_tag="SCK08_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1202760.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DARBFD010000244          872 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000244 DARBFD010000000
VERSION     DARBFD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.257"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>872
                     /locus_tag="SCK08_002947"
     CDS             <1..>872
                     /locus_tag="SCK08_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1202761.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFR"
BASE COUNT          302 a          166 c          161 g          243 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta ga
//
LOCUS       DARBFD010000245          868 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000245 DARBFD010000000
VERSION     DARBFD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.258"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            62..>868
                     /locus_tag="SCK08_002948"
     CDS             62..>868
                     /locus_tag="SCK08_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEG1202762.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEI"
BASE COUNT          301 a          132 c          159 g          276 t
ORIGIN      
        1 aggaagtccc ttctgtagaa tagagttacc acaacacatt cacagaaaag aggacttcca
       61 tatgaacgat tttactacag aaattatgga aatactaatc aataaaggtg atttggatga
      121 tttgtttcgt cgtcacctag aattggctat taacgcttta ttacaagctg aattaacggt
      181 atttcttgac tatgaaaagt atgatagagc tggatttaat tcaggtaatt cccgtaatgg
      241 gaattattcg cgctctttta aaacagagta cggtgaattg aatttagtaa tacctagaga
      301 taggaatgga gacttttctc aacaaacttt acctgcttat aaaagaacca atgactcttt
      361 agaaaccact attattcagc tagttcaaaa aggaattact atgtccgaaa tctctgatct
      421 aatcgaaaaa atgtatggtc attattacaa accacaaact atttcaaata tgagtaaaat
      481 tgtgtctgaa gatgtttcgg catttaaaga aagaacttta gaagctaaat actcagtcat
      541 ttttatggat gctactcata ttcccttaaa aagacaaacc gtatcaaaag aagctgtcta
      601 tatagtaatt ggtatcaggt tggatggaac taaagaggtt ctaggattta ctatagctcc
      661 aactgaatct gcttatgttt ggaaagagat tcttcaagat ctaaaagacc gtggtttaga
      721 agaggttttg ttagtcgtaa cagatggttt aagtggtatt gacgatagta ttcatagtgt
      781 ttatccaaat gctcaatttc aacaatgttg tgtccatata tctagaaata tcgctcataa
      841 ggttcgtgtt agtgatcgac aagaaatc
//
LOCUS       DARBFD010000246          839 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000246 DARBFD010000000
VERSION     DARBFD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.259"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..98)
                     /locus_tag="SCK08_002949"
     tRNA            complement(26..98)
                     /locus_tag="SCK08_002949"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:His,seq:gtg)
     gene            complement(101..174)
                     /locus_tag="SCK08_002950"
     tRNA            complement(101..174)
                     /locus_tag="SCK08_002950"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(140..142),aa:Trp,seq:cca)
     gene            complement(181..261)
                     /locus_tag="SCK08_002951"
     tRNA            complement(181..261)
                     /locus_tag="SCK08_002951"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(225..227),aa:Tyr,seq:gta)
     gene            complement(263..338)
                     /locus_tag="SCK08_002952"
     tRNA            complement(263..338)
                     /locus_tag="SCK08_002952"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(303..305),aa:Phe,seq:gaa)
     gene            complement(374..446)
                     /locus_tag="SCK08_002953"
     tRNA            complement(374..446)
                     /locus_tag="SCK08_002953"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(411..413),aa:Asp,seq:gtc)
     gene            complement(456..528)
                     /locus_tag="SCK08_002954"
     tRNA            complement(456..528)
                     /locus_tag="SCK08_002954"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(493..495),aa:Val,seq:tac)
     gene            complement(544..615)
                     /locus_tag="SCK08_002955"
     tRNA            complement(544..615)
                     /locus_tag="SCK08_002955"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(580..582),aa:Glu,seq:ttc)
     gene            complement(630..719)
                     /locus_tag="SCK08_002956"
     tRNA            complement(630..719)
                     /locus_tag="SCK08_002956"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Ser,seq:gga)
     gene            complement(759..832)
                     /locus_tag="SCK08_002957"
     tRNA            complement(759..832)
                     /locus_tag="SCK08_002957"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(796..798),aa:Asn,seq:gtt)
BASE COUNT          236 a          229 c          191 g          183 t
ORIGIN      
        1 ttggctatac cccaagaata aaaagggcga atgatgggaa tcgaacccac gaatgccgga
       61 gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tggcaggggc agcaggaatt
      121 gaacccacac taacggtttt ggagaccgct gttctacctt taaactatgc ccctataaaa
      181 tggaggagag tggattcgaa ccactgaacc ctaaggaacg gatttacagt ccgtcgcgtt
      241 tagccacttc gctactcctc catggtggcg cgggacagaa tcgaactgcc gacacacgga
      301 gcttcaatcc gttgctctac caactgagct accgcgccat attttaaaat tattaattga
      361 ataacaagaa cgacggttcc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg
      421 catgttaacc cctacaccac ggaaccagtt cattatggag gttaacggga tcgaaccgct
      481 gaccctctgc ttgtaaggca gatgctctcc cagctgagct aaacctccaa cattcaatta
      541 aaatgacccg tacgggattc gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg
      601 cttgaccaac gggccaggta aatttgctac ggagagtaag ggattcgaac ccttgagaca
      661 gtttggcacc atctacatga tttccaatca tgctccttcg gcctctcgga caactctcca
      721 gagtgaaaga agctcaatac tctttctcaa aatgagaact ccggcagtag gactcgaacc
      781 tacgacatca tgattaacag tcatgcgcta ctaccaactg agctatgccg gaataacat
//
LOCUS       DARBFD010000247          811 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000247 DARBFD010000000
VERSION     DARBFD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.260"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..672
                     /locus_tag="SCK08_002958"
     CDS             <1..672
                     /locus_tag="SCK08_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1202763.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DARBFD010000248          774 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000248 DARBFD010000000
VERSION     DARBFD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.261"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(109..>774)
                     /locus_tag="SCK08_002959"
     CDS             complement(109..>774)
                     /locus_tag="SCK08_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEG1202764.1"
                     /translation="RRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQ
                     EQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYS
                     LTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVH
                     QYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSG
                     EAA"
BASE COUNT          213 a          179 c          150 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gccg
//
LOCUS       DARBFD010000249          773 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000249 DARBFD010000000
VERSION     DARBFD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.262"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            91..>773
                     /locus_tag="SCK08_002960"
     CDS             91..>773
                     /locus_tag="SCK08_002960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1202765.1"
                     /translation="MSLNRYYEYYSNNNKVAGTKKINKINSITGQSLNFSYNKRKVLS
                     NISFSTEMNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDINMIDNESLRRL
                     ISIATQIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMTKLGITLDDNIYDE
                     KILLSGGEKQKISLARALIKSASVIILDEPFRNLDNETRDNLRDYILNTKKEVCYIII
                     THDHILDNF"
BASE COUNT          327 a           84 c          115 g          247 t
ORIGIN      
        1 aattttgtaa tagtatttat gtacgctaac atggtcccta ctttatggac tactttaatt
       61 agtattattt ggaaatggca agactattca gtgtcattaa atagatatta cgaatattat
      121 tcaaataaca acaaagtggc tggcacaaaa aaaataaata aaataaattc tattactggt
      181 caatcattaa atttttcata taacaaaaga aaggtattaa gcaatataag tttttcaact
      241 gaaatgaata aactattcat gatatcagga gaaaatggaa cagggaaaac aacactgatg
      301 aaattattta ctaaacagtt gccggttagt aataatcaat tattcgtcaa cgggatagat
      361 attaatatga ttgataacga atcattaaga aggttaattt caatcgcaac acaaatacca
      421 tatataatta atagctccgt gaaggagaat attttaatgg ggactaaaaa ctatgataaa
      481 aaaaaattca gtaaattaat agaactattg ggaataagta aatttatgac gaaattaggt
      541 attactttgg acgacaatat ttatgatgaa aaaatattat tatctggagg agaaaaacaa
      601 aaaataagtt tagctagggc gcttattaaa tcagcttcag ttattatatt agatgaacca
      661 tttagaaatt tagataatga aactcgcgat aatttgaggg attatatact aaatacaaaa
      721 aaagaagttt gttatataat tataactcat gatcacattc tagataattt ttt
//
LOCUS       DARBFD010000250          773 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000250 DARBFD010000000
VERSION     DARBFD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.263"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..526)
                     /locus_tag="SCK08_002961"
     CDS             complement(<1..526)
                     /locus_tag="SCK08_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1202766.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLII"
     gene            complement(602..748)
                     /locus_tag="SCK08_002962"
     CDS             complement(602..748)
                     /locus_tag="SCK08_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEG1202767.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          312 a          124 c           87 g          250 t
ORIGIN      
        1 gaatgattaa aaatattaag acaccaaaca caaaatgatc aaatacttga tattgatgag
       61 caacataaga aacaaataaa ccaattgtaa gtaaataaat catgaatata gcagtaagtt
      121 tagaactgga ctgttcaaca atcctattta tagctaattt tctatataac caaataaaca
      181 tcagcaaaaa caaagaatca agcattatct ggaatactac ataaccatac ccttttatcc
      241 cttcaaatga aaacatatac gtcataacca tggaagtaat aatagatctg aaatcatcaa
      301 taagcataca taatagtaat ttatttaaaa gcactatatt agttttcttc ttttccttta
      361 atataaaata acttccgatt ataggtatag ctgcggaaac atcagtaaat aattcaacaa
      421 aaaaagtgag tggtatcaat aaacatacaa aaagatattt tcttttgtta agatcagtat
      481 tagtcatata aaataagaga aaaaagaaaa agtttcccac gaacattctt aatagataag
      541 cttttgtatc gatagtaatc aagagatccc ctcctgattt taggatatca taaaaaaaat
      601 tttagttaca ctttttaaat aatgaaaaaa cacattccac taaatttgat gccggttttc
      661 cttgaggttt tgttccagca cctcctttaa tttttttaag ctcttgatct gaacattgtt
      721 tagcatttag tctatttttt tcttccatta cgattccccc tacaaactat aaa
//
LOCUS       DARBFD010000251          755 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000251 DARBFD010000000
VERSION     DARBFD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.264"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            330..728
                     /gene="spxA"
                     /locus_tag="SCK08_002963"
     CDS             330..728
                     /gene="spxA"
                     /locus_tag="SCK08_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1202768.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          278 a          106 c          135 g          236 t
ORIGIN      
        1 tactattaaa gaaataaaaa acataaaaaa tcttaagaaa tcggtttcat ttagcttttg
       61 agataaaata atagtacagt tttatacgct gtttcatatg tttagaaaat ttgaattaga
      121 tttgaaaact ctaattttta aatgataaaa aaagcgagaa tgctggtttt actgtatatt
      181 aaccttagat aactttcatt tttttaaaaa aattatgaaa aaaaggtagc tatttaaaag
      241 ttgattatgt ataatagaaa cagatgggag attattatga tcttctgtta caaaaaagtg
      301 attcttatca cgaaaaggag tggtgcacgt tgttaacatt atatacttcc ccaagttgta
      361 cttcctgcag aaaggctcgt gcgtggttgc aagaacatca gattccattt gttgaacgta
      421 atattttttc agaaccattg aacagttctg aattaaaagc gattttacaa atgacagaag
      481 acggaacaga agaaatcatc tcgacacgtt ccaaagtatt ccaaaaactg aacatggatt
      541 tagatgatct gccgttacaa gaattattgg aacttgtgca aaacaatcct ggattgcttc
      601 gccgtccaat catgattgat gataaacgtc tgcaagttgg ttttaatgaa gatgaaattc
      661 gtcggttcct accgcgcgat gtacgacaat tagaattacg tcaagcacaa ttgatggctg
      721 gattatagaa ggataatacg aaacttggaa aagac
//
LOCUS       DARBFD010000252          742 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000252 DARBFD010000000
VERSION     DARBFD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.265"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            132..344
                     /locus_tag="SCK08_002964"
     CDS             132..344
                     /locus_tag="SCK08_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202769.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>742
                     /locus_tag="SCK08_002965"
     CDS             506..>742
                     /locus_tag="SCK08_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEG1202770.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLK"
BASE COUNT          272 a          105 c          126 g          239 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aa
//
LOCUS       DARBFD010000253          733 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000253 DARBFD010000000
VERSION     DARBFD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.266"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>733
                     /locus_tag="SCK08_002966"
     rRNA            <1..>733
                     /locus_tag="SCK08_002966"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DARBFD010000254          714 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000254 DARBFD010000000
VERSION     DARBFD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.267"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..448)
                     /locus_tag="SCK08_002967"
     CDS             complement(<1..448)
                     /locus_tag="SCK08_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1202771.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          216 a          124 c           95 g          279 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aacc
//
LOCUS       DARBFD010000255          694 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000255 DARBFD010000000
VERSION     DARBFD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.268"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..448)
                     /locus_tag="SCK08_002968"
                     /pseudo
     CDS             complement(1..448)
                     /locus_tag="SCK08_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          208 a          140 c          105 g          241 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tatatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttcg ggtattaatt cgagaatcac acct
//
LOCUS       DARBFD010000256          688 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000256 DARBFD010000000
VERSION     DARBFD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.269"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(230..>688)
                     /locus_tag="SCK08_002969"
     CDS             complement(230..>688)
                     /locus_tag="SCK08_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1202772.1"
                     /translation="MIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYL
                     NFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIID
                     PSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          242 a          112 c           79 g          255 t
ORIGIN      
        1 aaaactaatc attgctccaa tcagtagcca agaaaaacgc attttaaaaa aatttttctt
       61 tataaaaacc tcatacaaaa ctataattcc accccaagca aaagctattt tcatcgggat
      121 attagttaca ttaaaagtac taggcataaa ataaagcaga caaatgatta aatttattac
      181 tcgaaacgca tctcgactta ataaactctt ctctaaaaca ggtactattc tattcatcta
      241 tatttccaac tttcaaaatt tctttatcaa ttctatttaa tagtttatca gagtaatccg
      301 atttaacttt tcttacaaat aatttttcac tttcttttat ttgatctata tccctttcat
      361 caaaccattt gttcatggaa ggatcaataa tatgaatatt tgctaatttc gaagtttgta
      421 aagtagtttc ttcaatattt ttcaattcaa gtggtaaatc tggtcctcca ataacatttt
      481 tatttttgaa cgaggaatta aaaaaaagtg tatgaaaaaa tttttcatct ggggcaaaca
      541 tacctatttt aaaattcagg tagtcttgtt gattttgttc atacatttgt attaattctg
      601 ttaggcattc tttcgacaaa gcccaccatt gagaaccatg atacaaatca aatttatggt
      661 gtctaatttt tgggggaagt ttaatcat
//
LOCUS       DARBFD010000257          688 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000257 DARBFD010000000
VERSION     DARBFD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.270"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            124..327
                     /locus_tag="SCK08_002970"
     CDS             124..327
                     /locus_tag="SCK08_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEG1202773.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            327..677
                     /locus_tag="SCK08_002971"
     CDS             327..677
                     /locus_tag="SCK08_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1202774.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          287 a           69 c          115 g          217 t
ORIGIN      
        1 tcaatcattg ttatataata aaatgttata taataaaatt actaatgata tttaatatta
       61 caagcaatct taagaaattc gaaaaggaaa aaatgattat gaaaaagaaa gaaaaagaaa
      121 gtattgaaac attgtgttat tctaggaata ttaggaactt gtctagctgg cattggtaca
      181 ggaatagacg ttgacgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa
      241 tgttgggtaa attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt
      301 tttggtagtt ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag
      361 tcatacatga attatacaat agtttaatga cacgacctaa tcagacgaaa gcattattag
      421 atattactga tgttttattt caggtatata aaaaaattga tacagcaaaa tatcctgaaa
      481 ttcttgtaaa taaattagtt aattatattt acatcgtggg ttttgataat aaaatacgct
      541 ttattgataa tgatgaaaaa ttattaatag aattagggga tatttctaaa aaagctggaa
      601 ttaatagtaa atacaaagca aactttacag ataaatcgca attttatagt tattcagaaa
      661 caatgcctca acgataaaat atagtata
//
LOCUS       DARBFD010000258          662 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000258 DARBFD010000000
VERSION     DARBFD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.271"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            122..457
                     /locus_tag="SCK08_002972"
                     /pseudo
     CDS             122..457
                     /locus_tag="SCK08_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            553..>662
                     /locus_tag="SCK08_002973"
     CDS             553..>662
                     /locus_tag="SCK08_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1202775.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          234 a          127 c          132 g          169 t
ORIGIN      
        1 tttatataat aaaatgcaat acttttcgag agtgtaaaat attttgtgta aatagaaaaa
       61 aggaagtccc ttctgtagaa tagagttacc acaacacatt cacagaaaag aggacttcca
      121 tatgaacgat tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga
      181 attattccgt gtccatttgg aaaaagctgt caatacgctt ctcaaaacgg agttaacggc
      241 tttcctcgat tacgaaaagt acgatcgcat tggttttaac acgggtaatt ctcgtaacgg
      301 ctcctatgac cgtacggtca agaccgagta cggggaactt catctccaga ttccgcgcga
      361 ccgcaacggc gagttcaagc aacagactgt tcctgcttat agacggacga atgatacctt
      421 agaggagacc gtcattcacc tcttccgaaa aggtatggat aggctacaat taactagtgg
      481 ataggctaca attaactaga cagaaaaatt aaggtgtgta gactagaaga aaacatacca
      541 ggaggaattt ttatgtctaa gagaacacga agaacttttt cacaagaatt caagcaacaa
      601 atcgtcaatc tttacttagc tggaaagcca cgtgtagaaa tcattcgaga atatgaacta
      661 ac
//
LOCUS       DARBFD010000259          635 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000259 DARBFD010000000
VERSION     DARBFD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.272"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..270)
                     /locus_tag="SCK08_002974"
     CDS             complement(<1..270)
                     /locus_tag="SCK08_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202776.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="SCK08_002975"
     CDS             complement(276..>635)
                     /locus_tag="SCK08_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1202777.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DARBFD010000260          627 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000260 DARBFD010000000
VERSION     DARBFD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.273"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..382)
                     /locus_tag="SCK08_002976"
     CDS             complement(<1..382)
                     /locus_tag="SCK08_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202778.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFE"
     gene            complement(389..>627)
                     /locus_tag="SCK08_002977"
     CDS             complement(389..>627)
                     /locus_tag="SCK08_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEG1202779.1"
                     /translation="DFNSSTEFKLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRG
                     TYIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
BASE COUNT          215 a           92 c           62 g          258 t
ORIGIN      
        1 tttcaaattc taaaaagcta tccgaatttt cttcgacgat taaatactta agagaattta
       61 tatcggaaaa gatagattca agtttactaa tctcattttc taaatctata ttttcacgtg
      121 tgattatctc atcacttttt cttaaaaagc taaaaattgc atcagttgct ttacactttt
      181 ccattaaaga agtatattct tcaatttttc ttctttcttt atggtcattt tttaaatcag
      241 tttcaatgtc ttttttcatt tgttctatac tgagtttgtt ctttttgttc ttttgagtaa
      301 atctaaaatt caaacacgaa aaaagtatgt tagcaataac acctataata gcaaataaat
      361 catttatact atttaatttc atttatcatc acaaacttag aactttactt atcatctcat
      421 aagacaagtt tccaaattga taattattgg tagattttaa atcattataa ttttcgagaa
      481 attttatata agtacctctc atcggtttat ggctattaaa agtgttaaaa tcactatctt
      541 caactatttc ttgaaaccta tttaattttt caataaaaaa ttgtgcataa agtgctaact
      601 taaattcggt agagctatta aagtcaa
//
LOCUS       DARBFD010000261          588 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000261 DARBFD010000000
VERSION     DARBFD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.274"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(65..150)
                     /locus_tag="SCK08_002978"
     tRNA            complement(65..150)
                     /locus_tag="SCK08_002978"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="SCK08_002979"
     tRNA            complement(167..238)
                     /locus_tag="SCK08_002979"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="SCK08_002980"
     tRNA            complement(250..322)
                     /locus_tag="SCK08_002980"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="SCK08_002981"
     tRNA            complement(340..421)
                     /locus_tag="SCK08_002981"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="SCK08_002982"
     tRNA            complement(429..501)
                     /locus_tag="SCK08_002982"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="SCK08_002983"
     tRNA            complement(505..577)
                     /locus_tag="SCK08_002983"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DARBFD010000262          585 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000262 DARBFD010000000
VERSION     DARBFD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.275"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>585
                     /locus_tag="SCK08_002984"
     CDS             <1..>585
                     /locus_tag="SCK08_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1202780.1"
                     /translation="PVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKI
                     PHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNFHLDSTHIKANANKHNF
                     TKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYV
                     KGEQEKQFAYSAHTSCDDNGFILSTIITPGNIHDS"
BASE COUNT          200 a          110 c          114 g          161 t
ORIGIN      
        1 ccagtgattc tgatcaaatt ggtgtttatt cagtatttat tcggtatccg ttccatgcga
       61 caaaccatta aggaagtcga taccaatgtc gcttaccgct ggtttttagg ctactccttc
      121 gaagagaaaa tcccccattt ctcaactttt ggaaagaatt atgttcgccg atttagagaa
      181 acgacggtgt ttgaggatat ttttgcctat attttagaac aagcagttaa agcaggtttt
      241 gtaacagagg ataattttca tctagattct acccacatca aagctaacgc caataaacat
      301 aattttacga aagaaatgac ccatgatgaa gcgaaagcct atcaggatga attagaagat
      361 gagatcaatc gtcaacgaat agaggctgga aaacgccctt ttactttgga cttagaaaag
      421 gaagtgaagc tgaaagaaag aaaaataagt aaagctgatc cggaaagcgg ctactacgtc
      481 aaaggcgaac aagaaaaaca atttgcttat tctgcccata cttcatgcga cgacaatgga
      541 tttattctta gcacaatcat cactcctgga aatattcatg acagt
//
LOCUS       DARBFD010000263          570 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000263 DARBFD010000000
VERSION     DARBFD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.276"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..276)
                     /locus_tag="SCK08_002985"
     CDS             complement(<1..276)
                     /locus_tag="SCK08_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1202781.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          177 a           95 c           92 g          206 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgatatatta aacaacgttg tcgcgcttca ttgaaacatt ttatgaaaat
      421 tccaaaaaag agttgacaca tgtttttaga cgtgataaga taactaagtc gttaaaaaga
      481 accacttgcg aagcttgatt ctatcgaaaa aaaacttcaa aaaagttctt gacaaataac
      541 aaacgataag ttatgataaa tgagttgctg
//
LOCUS       DARBFD010000264          526 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000264 DARBFD010000000
VERSION     DARBFD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.277"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCK08_002986"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCK08_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEG1202782.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="SCK08_002987"
     CDS             complement(349..>526)
                     /locus_tag="SCK08_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202783.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DARBFD010000265          515 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000265 DARBFD010000000
VERSION     DARBFD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.278"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(107..>515)
                     /locus_tag="SCK08_002988"
     CDS             complement(107..>515)
                     /locus_tag="SCK08_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEG1202784.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcca
//
LOCUS       DARBFD010000266          514 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000266 DARBFD010000000
VERSION     DARBFD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.279"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..132)
                     /locus_tag="SCK08_002989"
     CDS             complement(<1..132)
                     /locus_tag="SCK08_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1202785.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DARBFD010000267          494 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000267 DARBFD010000000
VERSION     DARBFD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.280"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          166 a           96 c           58 g          174 t
ORIGIN      
        1 aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata aaaaaattat
       61 tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc tttcatcact
      121 tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc gtattccttc
      181 aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac ttaaaaattt
      241 agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa ttgacagacc
      301 aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat tcattttcaa
      361 gacatctcga ttcatattca agataggaat ctttttatca acatcacttt attcgtattt
      421 tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact attcagccca
      481 ttcaacgacc aatg
//
LOCUS       DARBFD010000268          491 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000268 DARBFD010000000
VERSION     DARBFD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.281"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>491
                     /locus_tag="SCK08_002990"
     CDS             <1..>491
                     /locus_tag="SCK08_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEG1202786.1"
                     /translation="VGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEYGKLLYQI
                     WKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKR
                     LVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRN
                     KFYR"
BASE COUNT          174 a           84 c           94 g          139 t
ORIGIN      
        1 gtgggctact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat cttatatgat
       61 cgtggcatta acgtttctca tacgacgatt tatcgttggg tgcaagaata tggcaaacta
      121 ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt attcatggaa aatggatgaa
      181 acgtacatca aaattaaagg aaaatggcat tatttgtatc gagccatcga tgcagatggt
      241 ttaaccttgg atatttggtt acgtaaaaaa cgggacacac aagcagccta tgcttttctt
      301 aagcggttag tgaagcagtt tgatgaaccg aaggttgtag tcacagataa agccccctct
      361 attacaagtg cctttaagaa actaaaagaa tacggctttt atcaagggac agaacatcgt
      421 accattaaat acctgaataa tttgattgaa caagaccatc gtccagtaaa gagacgcaat
      481 aaattctatc g
//
LOCUS       DARBFD010000269          485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000269 DARBFD010000000
VERSION     DARBFD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.282"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..259)
                     /locus_tag="SCK08_002991"
     CDS             complement(62..259)
                     /locus_tag="SCK08_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1202787.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DARBFD010000270          482 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000270 DARBFD010000000
VERSION     DARBFD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.283"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            143..400
                     /locus_tag="SCK08_002992"
     CDS             143..400
                     /locus_tag="SCK08_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202788.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          205 a           63 c           69 g          145 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaatag
      481 at
//
LOCUS       DARBFD010000271          461 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000271 DARBFD010000000
VERSION     DARBFD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.284"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>461
                     /locus_tag="SCK08_002993"
     CDS             <1..>461
                     /locus_tag="SCK08_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202789.1"
                     /translation="GTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQ
                     PITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKY
                     TWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          187 a           68 c          108 g           98 t
ORIGIN      
        1 ggaacaagta ttgaaagtgg aacaacagtc tttgcagtga aagacggcgt aagcttacca
       61 gaagataaac ttccagtgtt gaaagcaaaa gatggttata cagatgcgaa atggccagag
      121 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
      181 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      241 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      301 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      361 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      421 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DARBFD010000272          452 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000272 DARBFD010000000
VERSION     DARBFD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.285"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>452
                     /locus_tag="SCK08_002994"
     CDS             <1..>452
                     /locus_tag="SCK08_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202790.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DARBFD010000273          415 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000273 DARBFD010000000
VERSION     DARBFD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.286"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..339)
                     /locus_tag="SCK08_002995"
     CDS             complement(<1..339)
                     /locus_tag="SCK08_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1202791.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DARBFD010000274          401 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000274 DARBFD010000000
VERSION     DARBFD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.287"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          112 a           98 c           76 g          115 t
ORIGIN      
        1 attataaata actcataaaa aatattttga ctttcaaaat cacttttgct accagaagtt
       61 gaggcgaagt gttttaagca tagttgtacc ctagtttgac cgttaaaaat aaataacgat
      121 cgctagagtg caactatgtt tgtttgccct accgtcagcg tcggcttggc ctctcacatc
      181 cattaccgtc gggattatct ccgttacata cccttacaaa atacgccctg taagcattgc
      241 atgaaaccac cgtgcatttg caccgtattt ccgccctagc cgttcaaatc ggtcgctacc
      301 accattcaga aacacgtgcg gtaatggaag gtttggagga agctatccag accactaacg
      361 attgtcccta tcgtcgtacg tttcactcag atcaaggttg g
//
LOCUS       DARBFD010000275          376 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000275 DARBFD010000000
VERSION     DARBFD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.288"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          120 a           93 c           85 g           78 t
ORIGIN      
        1 atgtaataag tggggggagg gctgtcggcg cttggtatga aaggcattct ccgacttcca
       61 ggtttttgac cattttctct caacgtggaa aaaccgccac ttatgagaga aaatggtcaa
      121 aaacctaagc gggaagtctc cgaataacat accaagcgcc gacagccaac agataaagaa
      181 tacactacta aaaatagtag tagcataaaa tttaaaaggg aaaaaatcaa cccctacccc
      241 agtcttgggg tctcaaaact aactcccccc taaccccctc tttcagcctc ttttgaaaga
      301 gggggagaat caaccccacg gccaacgccc cagtgttctt ggggctgtcc agaacagaaa
      361 cttgcgagag agtggg
//
LOCUS       DARBFD010000276          375 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000276 DARBFD010000000
VERSION     DARBFD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.289"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>375)
                     /locus_tag="SCK08_002996"
     CDS             complement(<1..>375)
                     /locus_tag="SCK08_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202792.1"
                     /translation="YTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTT
                     GSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKV
                     STSYTVTTIISYLVTLTAYLCL"
BASE COUNT          117 a           68 c           53 g          137 t
ORIGIN      
        1 aataaacata aatatgcagt taatgtaaca agataagaaa taatagtcgt tactgtataa
       61 cttgtgctta ctttaatatc agcaactcct ttgactccgc ttatttgtac caatccatta
      121 gcattcacct gctcctttac aggttgatga tttatttcta cattcgttcc taaataagca
      181 tatacaggaa gtgtgattac ttggctcttc tcattagtta ctctatatat gatagaacta
      241 ccagttgttt tataaggaag gtttacccat tgactgccaa tctttgcttc gtgttttatt
      301 atatgctcct tattctccaa tgaagcgata ggagaataat catataaaaa ataatccatt
      361 cctttagtgg tatac
//
LOCUS       DARBFD010000277          367 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000277 DARBFD010000000
VERSION     DARBFD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.290"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          134 a           67 c           34 g          132 t
ORIGIN      
        1 tttccagtca tcaaataaaa gtaaatttca gcttctcatt gtatatagaa atttcaaaga
       61 aactattctc tcaatttctt taagttaatc cctgcaagat tattttagaa actacttttt
      121 tcatataacc agaataattt aactattcac tctaacagtt aaattatttt ctcccatcac
      181 atgactagaa gcacatcttc tcaacaaact ttttcaaaat gatcactcat ataattacac
      241 cttcattaat ttaaacagca ctacaaattc caataattaa tgagcatatc gtatttgtat
      301 acaaaaaaat cgttgttaag gcaaaaatag ccgtttttac gctcatttta tatactgttg
      361 gaaggtg
//
LOCUS       DARBFD010000278          358 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000278 DARBFD010000000
VERSION     DARBFD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.291"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            78..>358
                     /locus_tag="SCK08_002997"
     CDS             78..>358
                     /locus_tag="SCK08_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1202793.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
BASE COUNT          122 a           58 c           73 g          105 t
ORIGIN      
        1 tttatttttg aaaacttgcc atacttttta tttgttttat tttccagtat actatacgaa
       61 aggaaggtgg ctagaccatg aaagttgtac aaacaaaaga tacaatgagc aatatttact
      121 tagatgctgt acggatcagg catcaagttt ttgtagtcga acagggtgtg cctttgtctc
      181 gagaaataga caaagatgaa gcccattgta tccacttcgt cctgtactca gataaaaaag
      241 aaccgcaagg aactgtacgt cttttgcctt tagaaaacgg aaaaatgaaa ttacagcgga
      301 tggcgattct ttctgaatac agacaccaag ggttaggaaa aatattgatt gaagaagc
//
LOCUS       DARBFD010000279          338 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000279 DARBFD010000000
VERSION     DARBFD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.292"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..338
                     /locus_tag="SCK08_002998"
                     /pseudo
     CDS             1..338
                     /locus_tag="SCK08_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DARBFD010000280          331 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000280 DARBFD010000000
VERSION     DARBFD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.293"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>331
                     /locus_tag="SCK08_002999"
     CDS             <1..>331
                     /locus_tag="SCK08_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HEG1202794.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DARBFD010000281          323 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000281 DARBFD010000000
VERSION     DARBFD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.294"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="SCK08_003000"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="SCK08_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEG1202795.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DARBFD010000282          312 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000282 DARBFD010000000
VERSION     DARBFD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.296"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>312
                     /locus_tag="SCK08_003001"
     CDS             <1..>312
                     /locus_tag="SCK08_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1202796.1"
                     /translation="GDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQW
                     FSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRN
                     GL"
BASE COUNT           97 a           63 c           55 g           97 t
ORIGIN      
        1 ggtgatacga tcgttggcat tcatcataaa agtgccgtca ttactttagt tgaaagatta
       61 tctaaagtca ttatcacgat taaacccaac ggccgtaagg cattagatat tgaaactgcc
      121 cttaatcaat ggttttctcg cttccctaaa aacttcttta aatctattac gtttgactgt
      181 ggaaaagaat tttctaactg gaaagccatt agtaaccaac atgatattga tatatatttt
      241 gcggaccctg gaacaccttc tcaacgccca ttaaacgaga attctaacgg gattctgcgt
      301 cgtaatggac tg
//
LOCUS       DARBFD010000283          309 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000283 DARBFD010000000
VERSION     DARBFD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.297"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..309
                     /locus_tag="SCK08_003002"
                     /pseudo
     CDS             1..309
                     /locus_tag="SCK08_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DARBFD010000284          308 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000284 DARBFD010000000
VERSION     DARBFD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.298"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(76..>308)
                     /locus_tag="SCK08_003003"
     CDS             complement(76..>308)
                     /locus_tag="SCK08_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1202797.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DARBFD010000285          300 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000285 DARBFD010000000
VERSION     DARBFD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.300"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>300
                     /locus_tag="SCK08_003004"
     CDS             <1..>300
                     /locus_tag="SCK08_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1202798.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSI"
BASE COUNT          117 a           57 c           48 g           78 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
//
LOCUS       DARBFD010000286          292 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000286 DARBFD010000000
VERSION     DARBFD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.301"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..185
                     /locus_tag="SCK08_003005"
     CDS             <1..185
                     /locus_tag="SCK08_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1202799.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DARBFD010000287          290 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000287 DARBFD010000000
VERSION     DARBFD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.302"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT           96 a           49 c           45 g          100 t
ORIGIN      
        1 catatttttg aaaaaaagtg agaaaaaagg cttacaattg gagtggtagt taattagtga
       61 cttatttttg attttatagc actgatacta taaaatatag atatcatcat attacacaat
      121 cttaatacta acttaaaaaa tatctccttt cacaagtatg gtgataaaat tcgttccggg
      181 ctaccttttt aggtagccta ctttaatctt tatacctttc tggatcaacg aaagtatact
      241 ttatatagtc ataacgccga tgatcgctac gtgcgtccgg cacgtcagtc
//
LOCUS       DARBFD010000288          289 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000288 DARBFD010000000
VERSION     DARBFD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.303"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..170
                     /locus_tag="SCK08_003006"
     CDS             <1..170
                     /locus_tag="SCK08_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1202800.1"
                     /translation="YLPEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQ
                     LYTPPRSVIEK"
BASE COUNT          104 a           61 c           45 g           79 t
ORIGIN      
        1 attatcttcc tgaatacaaa gctttttatc aaagtaaata taaggaggtt ccaaagcatc
       61 aacacaaacg tgcactcgtc ctaaccgcaa gaaaatttac gcgtctggtg gatacgctac
      121 tacgtaaaca ccaactctat acgccaccaa ggagtgtgat agagaaataa caatccgtta
      181 ttgacgcaaa tccttgaatc aacccgaaaa aaatttgatt ttgatcgggt ctagtttcgt
      241 gcgcttcaaa acatattact caataaagaa aatcttaaat ttcatcttg
//
LOCUS       DARBFD010000289          282 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000289 DARBFD010000000
VERSION     DARBFD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.304"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>282
                     /locus_tag="SCK08_003007"
     CDS             <1..>282
                     /locus_tag="SCK08_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEG1202801.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DARBFD010000290          273 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000290 DARBFD010000000
VERSION     DARBFD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.denovo.305"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(133..206)
                     /locus_tag="SCK08_003008"
     tRNA            complement(133..206)
                     /locus_tag="SCK08_003008"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Ile,seq:cat)
BASE COUNT           95 a           60 c           50 g           68 t
ORIGIN      
        1 acatcctttc attaatccat aaaaggatac ataaaaaagc cactcatttg agtggcaatg
       61 aagaaaagct ttagcttgta taatactctt caaaaaaatt ctaacacaga acgattcaaa
      121 tggctacgtt aatgtaccct gtaggactcg aacctacgac cggacggtta tgagccgtct
      181 gctctgacca actgagctaa gggtactggt tgttgccaca taaagccata aacaatcaac
      241 cagtagaatg tgtggcaaca aacctgttat cgc
//
LOCUS       DARBFD010000291        11676 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.guided.083, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000291 DARBFD010000000
VERSION     DARBFD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.guided.083"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            18..515
                     /gene="dfrG"
                     /locus_tag="SCK08_003009"
     CDS             18..515
                     /gene="dfrG"
                     /locus_tag="SCK08_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1202802.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(587..2539)
                     /locus_tag="SCK08_003010"
     CDS             complement(587..2539)
                     /locus_tag="SCK08_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1202803.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2546..2782)
                     /locus_tag="SCK08_003011"
     CDS             complement(2546..2782)
                     /locus_tag="SCK08_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202804.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3017..3820)
                     /locus_tag="SCK08_003012"
     CDS             complement(3017..3820)
                     /locus_tag="SCK08_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1202805.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(3836..3952)
                     /locus_tag="SCK08_003013"
     CDS             complement(3836..3952)
                     /locus_tag="SCK08_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG1202806.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(4197..5126)
                     /locus_tag="SCK08_003014"
     CDS             complement(4197..5126)
                     /locus_tag="SCK08_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1202807.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(5143..6165)
                     /locus_tag="SCK08_003015"
     CDS             complement(5143..6165)
                     /locus_tag="SCK08_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1202808.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(6162..8189)
                     /locus_tag="SCK08_003016"
     CDS             complement(6162..8189)
                     /locus_tag="SCK08_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202809.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(8186..10639)
                     /locus_tag="SCK08_003017"
     CDS             complement(8186..10639)
                     /locus_tag="SCK08_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1202810.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            complement(10623..11018)
                     /locus_tag="SCK08_003018"
     CDS             complement(10623..11018)
                     /locus_tag="SCK08_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1202811.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            complement(11090..>11676)
                     /locus_tag="SCK08_003019"
     CDS             complement(11090..>11676)
                     /locus_tag="SCK08_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202812.1"
                     /translation="GWVIDSFYFRYKQQNYIVLVKLFEKEEKVPEYALLKLEFLKEND
                     FSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQFSQTLARFIPTEVSEKKNE
                     DQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKRSPFNDNKTRLYRPSLYER
                     LGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
BASE COUNT         3441 a         2271 c         1976 g         3988 t
ORIGIN      
        1 aaaaggctgt gttataaatg aaagtttctt tgattgctgc gatggataag aatagagtga
       61 ttggcaaaga gaatgacatt ccttggagga ttcccaagga ctgggaatat gttaaaaata
      121 ctacaaaggg acatccgata atattaggta ggaagaacct tgaatcaatc ggaagagcct
      181 tacctgacag aagaaatatt attctgacga gagataaggg gtttaccttt aatggttgtg
      241 aaattgttca ttcaatagaa gatgtttttg agttatgtaa aaacgaagaa gaaattttta
      301 ttttcggagg agaacagatt tataatttgt ttttccctta tgttgagaaa atgtacatca
      361 caaaaataca tcatgaattc gaaggagata ctttttttcc agaagtgaat tatgaggaat
      421 ggaatgaggt atttgcccaa aaagggataa agaatgataa aaatccgtat aactactatt
      481 ttcatgtata tgaaagaaaa aacttattga gttaacgggt gcgttaattg aagaagaaca
      541 gtaaagataa agcacaagag cttgggatta ctccaagctc tttttgtcat ttgaaataaa
      601 ttatatcttt ccaatcaaat tgtttatctg taatccctaa tctttgagca ggtgtcaaaa
      661 cctttttatc cgctgattta aaagggcgac aaaagttgta gtaagttctt agaattgtaa
      721 tcgcaaattg agcatacttc ggattgaagt tagcgtaaat atagctcttc ttatcaccac
      781 gagctgttgt taatggtctt tcaagagatg atatataacg tcttatctgt tgaataaagc
      841 tattggtcga atggtcatta acatttaata gcatctttgc gatttcgttt ggttccagag
      901 cagataaatc cgttctacaa tcaactgaat aaaatccttt atccttagtg gctaatggat
      961 gtttgatagg gttatcggca tatttgcgat atctttctcc atctttacta atagcctctt
     1021 cgtggaattg atgacttgtt agtaattcag ttagataatg agatgccaaa gtccgtagaa
     1081 actttgtttt gaatcctctg atatctcccc aatcgagcag ttctgctttt gcttgtccga
     1141 attcttttaa gcattgtttt ctgcttttcg tcttgttaac ttggcaaata aaatgatgtg
     1201 catcgctcaa ccttaattct ttcgtgaata ctctataaaa ggcgtttcta atagagaagt
     1261 catcatcact aatcattcgc cattcgtcgg cattaaccat ttgtttaatt aaccaatagt
     1321 gagcagttgt agtataagga gcatcaacat ttaaaccatc gacaaatcta ctcctattag
     1381 taattttccc taattcgtga agataagcat tcctattttc gctatcgtta gcggatggtt
     1441 cttgtggata ataagaccaa tctaacctat catttttacg actaaaagta ttgagatggt
     1501 cttctttgaa ttttcttgta tcttcattta attcatccat tgaaatattc caatcatagg
     1561 caacatcaga acggaacaca tatcttgata agacctcagc agatacaact atatacgttt
     1621 gcatattaag gtcttcaaac cccgtatatt tctttgaacc ttgtcctttc ttacggacgt
     1681 tatttaggta gtagtgcatc ttgtccgtat taagccacat ctcgttaaat ttctttgttt
     1741 gaaggggttg tgtctcatat cgttctaaaa actccaaaca acgcctatac agccactcca
     1801 gcttatgata gtaggttcca actccaattc ctaaaatatc acaagtacga ctgactgaaa
     1861 cccttccaac aaccatctta gcgaacattg gaagaatggt gttcttttgt tggtgataag
     1921 tagtggtttc ttctcttttt ggtagaacat tagtgaactt cttacacgcc ttacattgat
     1981 agcgttgaga tttgccccta cttttccccc ttttgtaaaa ttgcttcggc tcattgaatg
     2041 gggttgattc ttcttccgag cagccttctt tatgaaattg ataatctggc tcaacatctt
     2101 ggatgctatt gatttctata aggcgtttaa tttcttcaac aacagaccaa ttagataaag
     2161 gggttactgt acagttttgc gatacacctc tgccaatagg attgtcattg cagtacatac
     2221 ctttatgacc tttatctttg gaactaccag tcattttata tcgacttggt ttccctttta
     2281 catctttgaa tttgtgctgt tctttcccaa aattacaaca aaacggattg atgcagtaat
     2341 tatattgaat gggatattca gaaccgttcc atttaaaaga cgttggcaag aacaacattt
     2401 tgttatacct tgttgcaaat cgttttggag ttaataaatc atattgttta gccctatctt
     2461 ttaattcttc gtttgaaatc ggaattttaa catctacaat ttcatcttcc ttcgtggcta
     2521 atcttttaag cttggtcatt gtctatcaac ccactctatc tttgataccc attttttcaa
     2581 caatccgctt attacttcgc ttgttggcaa tccattcaat aaacttctta tcgtctaaaa
     2641 tgttcaataa gaattcatcc actgcttcgc tttcagtacg ctctgcatat tcagcgtatt
     2701 gcttaactat cacccttact tgctctgata tttcccaatc cacagactta gcgttcttat
     2761 tttttggctc aagaaattgc ataggaaaat gcaccccttt ttgcttagta aattgtaacc
     2821 attttaacac accaatttgc attcctacaa taattatttc acgttcgcat ttttatatac
     2881 attttaactt acattttgtg gtgtaaatta acaacaaacg ttgatttaac aataaaaaac
     2941 gagtatcaaa ttcagttgca atttgatact catttaggtt tacattttca ctttttgtac
     3001 tccaaaactg aactacttaa tatgcaagac gttgtctttt ttttgtatat tcaattttga
     3061 aaacatttcc gcaaagttca gacggacctc gatgtgttga tgaataaaat ttattagtaa
     3121 taacttctat aaggttatca atccctatat tactttttgc acttccatga taaagaggga
     3181 acagagaaca attctgaaat cttatgcttt cctcttgttc gagttccaat gcttctaatg
     3241 atttaccgga catatatttc tctaaaaggt catcgtttcc ctctattacc gtatcccatt
     3301 gttcagattc ggtaaagttc gtcacacaca tattaggata cagttctacc ttctgtttga
     3361 ttacaatttc ggcagaaagt ttctctttaa tatcctgata aaccgttgat aaatcaattc
     3421 cattttggtc aatcttattg ataaaaaaga ttgtgggaat ccccattttc ctaagtgcat
     3481 gaaataatat acgagtttgt gcttgtacgc catcttttgc agaaatcagt agaattgccc
     3541 catctaaaac tgataatgaa cgatatactt ctgctaagaa atccatatgt cctggcgtgt
     3601 ctatgatgtt caccttcgta ttttcccact gaaaagaggt tattcctgtc tgaattgtaa
     3661 ttcctctctg acgttctaaa agcgtattat ccgtcctcgt tgtacctttg tccacgcttc
     3721 ctaattctgt aatcgctcca ctgttatata ataagctttc tgttaaggta gtttttcctg
     3781 catcaacatg agctaaaact ccaatattaa taattttcat gtgattttcc tccattcaaa
     3841 aacccaaaag ggcataaaaa tcccagtgat aaatactttt atcactggga tttttatgca
     3901 taaccatagg tatacaaagc atacagatat tctctggata ctttagaatc acatgataaa
     3961 ggtattctta aactgggtac aaaaaactaa gccctcctaa aaaaggacat ctaattattt
     4021 gttcccgcta tcaaattgac agtttattta agaatacctt gccgcatatt tattaactcc
     4081 ttttaaatag tcacttaaat aatagcacgt aagagcatat ttgtaaagga atctccaatt
     4141 ttttatcaaa aagagtacat gattacaaag tatctgtaat catgtaccaa tatttgttat
     4201 tttacaatct tccaattatc ttgtttttct aatatcaact caaattgcga gatttgggtt
     4261 gcctttgttt cttgatctaa atatttcaca gcgacattca cgattacttg gttatctttt
     4321 ctggtaaaga taggattcac caattcctcg aaaacatatt ccttgttaat cataggtaac
     4381 acatgattac ttacatagta agtcaattct ttttctgtgg cggtaggata gagctggaag
     4441 aacgtttcta aaaagctagt gatttcgtcc atcgtttctg tatctactgt atggtcactt
     4501 tctagctgtt tgggttggta gtccgatttt tgaggtttct tactcattgt cggatttttg
     4561 ataataacca tattatctga ttcatctata tggacaacca catgaaagct agacgatata
     4621 gtttccttgt ctttgtcttc ggtaataact tgttcaacag aaaacagtac ctcaaaggtg
     4681 ttctcattca cttgagaaac ctgccacacc tgtatatcgt tcacactaga actagtagga
     4741 atatccttac gaatcatttc ttcatttaat acctgcaatt cttccgtcaa ataatgggtc
     4801 aatttttcat ttcgtttatc aatggcttct tgggattgtt gccacgaaaa gtagtctttc
     4861 gcaaaggatt tcacaaaact ttctacttgg ttggtatcga caatctgctg tttaatgatt
     4921 tctgtttcac ggacagtatg agtgtcaatg gcagtaaaat ttttatagat tccaaatccg
     4981 acactaccaa tcaataaaac ccataaaaca aaggttaatt tcttatgcgt gccaacttta
     5041 atcatcggaa cttttctttg cttgctcggt tttttagatt tctttggttt ctccttcttt
     5101 tcttttcgct cgatttttat aatcatttga ttcattcctt tcttagttat gaataacacg
     5161 tctagcccca atcagatggt cttgccaata acgactgctt aagtcgccat aaccgattgg
     5221 gtcgcctgca tgataaaagc gatttccctc taaatatatc gccacatgag tcacatatgt
     5281 gcctgcatta taggttgaat gaaagaagat aaggtcgcct gcttgggctt cttccataga
     5341 gatttcttgc gtagcgtcat actgatcttg ggcgacacga gggagagaaa tgccagcttt
     5401 gtcatacacc cattgtatta agccactaca atcaaaagag gtggtaggcg acgcccctcc
     5461 aaagacatag ggaaaccctt cgtatttcaa ggcttcgtcc ataatgactt gcacggtttc
     5521 atcatcaaat tctgttgggg aagtgtcggt taggtattgt gaaaccaact gaacataaaa
     5581 ctgatttcca tagttatatc gccaaccacc attcacaggt atggctatag gattgggata
     5641 gtctgctttt tgaccacccg acttctcttt agaaaactgt tcggctaatt cataggtata
     5701 ttttttccca tgactcctca cataatttaa aaagccaccg ccataattat aagattggat
     5761 aacagaatct atatccactc cttgttgttc tgcactggtt aataattcac tgaaatattt
     5821 aaccccttgt ttgatggact cttcggtact caaactgttg ggcggtaaac ccaaagattc
     5881 ggaactttgc ataacatctt ctgctgtacc gcccgattcc acttgcataa tggctaagat
     5941 ataagaaaca tagtcttcaa tcccatattc cttaccatat ttttcaatca acggacggtg
     6001 tgccaacact tctggtgaaa cggtcacacc gccttgtgga atagaagaat ccttgttgtt
     6061 ttcgccactg tccgtatcat ctgcaaagaa aatgactagg aataataaga gaaaaaagaa
     6121 gagagagaag accacactac aaatcactgt tatttttagc ttcatgtttg ttgtcctcgc
     6181 ttatccgttc ttttatttag cttcctctga ttgaattgct tcggtggtgt cttttgtttt
     6241 tgctcttttt tcaatggtcg ctgatgattt tcacgtttag tgttttgtga caaaacaggc
     6301 gtgacggttt gctgacgctt gcgaacatgc tgctggttat tctgaacagg agatgatggg
     6361 ttttcttttc gtcttttttc ttttgcttta tccagctcca aacgcttttg agcaatgttc
     6421 tttcgttttt gctcctgctg gttcatacgc tgttgtttgc gttgagcttt ggtatctgcc
     6481 atagattgtt taaagtctgt tacattatga gtgacattct cttttccttg atgaagggca
     6541 tatttgacat gggttggtgt cgcctgtatc tgttgttttg cctgctttct tttattgact
     6601 agacgatttt tcgtgtccaa tacatgggct gtcttttcac ctaatcgttg cccgataccg
     6661 ccttgttgct ttttcacatt ggaaagtgct gtttctttct ttggtgtagt ccgttcattt
     6721 tgacgagtat ggttagcttt attggtttga ttctgctttg ttgtcattgt cttttgcttc
     6781 gttgaccctg cttttccacg aaaaatctta ctcatactgc gttgaaatct tctggtttgt
     6841 cgattcatca tttgacgagg tctgcgaaac acttgtctgc ccatgttttg agagtcgttg
     6901 ctttgtaaag tgaataggct cattaaatcg cccagtttaa aataaatccc tgcaaaggtc
     6961 acaatttgta aaaacataat catgaaaaag ggagaacttg ctgacagcga ataaagcata
     7021 gtggaaatac taaaggcaac ggtaataatc aaggtaatac cagcccttgt cataatcaca
     7081 ttaaataact tggtaatggc atgcctgccc atgccatcaa aactcggcaa catacttaat
     7141 aggaaactga ttggcaaaaa catagcgaaa ataataaata gcacttgcga aaaaatcatg
     7201 attcctgtta gaaggaaaac aaaaatcgag ataccaatat tgaatacaaa caaaaagaag
     7261 actgttccta atctggaaat cgtcttcggt agggttaaat aggtattgtc atgatcttca
     7321 atttcttcaa tcactacatt ttctctatct tcaccattgt ttgtatctgg actggtagac
     7381 aataaacttt ccacacgttc ttcccctaac gcttctacat cagtggagcc atattgaagc
     7441 aatagccatg gttgctttac ttgaacagag aataagctgt cacgtatcat gtccacactg
     7501 tctttccctt gactatccgt gttaggaaga acaatttttg tcccaatatt cagactggcg
     7561 gtactgacat ctttggaaaa atcattgatt ttcgcaatat aatcgggtgc atacgcaata
     7621 aaggaagccg atagaataaa caccacgaca aagtttaaaa cagctcgtat cactttcgtc
     7681 gtttctcgct taaccaatcc tgtataggca acatagattc caacaatgag aataaaaaga
     7741 aggaggaaac cgacataaaa ccctgaactg gaaaagccat tcggtgtaat gcctgcaatc
     7801 gtctgcatgt tttcgccaat tgcttgggat gtttgactaa tgaaatctaa ggcataggct
     7861 tctttcacca aataacctgt ggcgttcgat agataaagag aaattgtcca aataaaatta
     7921 gtaatggcat acaatccata catcaccgat tgtcctatgc catcactcca attccatgga
     7981 agccagcccc agctattgtc cacataataa tcaagctgat attgattcaa accatacttg
     8041 gagtattcat tgcttgtatc aatggtttca tcaactaagc ctgtcgcttg tactgcattg
     8101 cccagcgtgc ctaataaaag taaaataacg cccacacaaa ggagcgttat ccaactatag
     8161 tgaagaaatt tcttttgttt ttgtgtcatt cttccacctc atttcgcaca ggcggtctgg
     8221 tatcaaaggc atgaagcaac tcttcaaaaa tcgggtgaaa ctgcataacc cctacacgac
     8281 catagatgtc tgaaactaaa cattgtccat tttctaattc acgtaagcgt ttctgattgt
     8341 tttcatcttc cttgtccaca ccaaaaaatt ctaaggtctt tttgatttcc actaggtcag
     8401 tggaacgaaa ggcaaatttc aagccaatat tattcttcat cttttcatct agtaaatcgt
     8461 ccgcgttttg agtaacgaaa tagacccctg cattcatggc acgtcctgct cgaaccaatc
     8521 gcatagatag agcttttcct tgtgccactt gtaaaaaact ccaagcctca tcaaggtcaa
     8581 caattttaaa aatggaacgg tcagagtgga taaagtctaa agcaaaggta ctaatgacaa
     8641 tcagcattgc cacacttaat aattccatgg tagtgtattc ttcaaaagag ctattggaat
     8701 cgggtaagac caaatctgcc acttgaataa tattgagttg tttttccaag ctaatggatt
     8761 gggtgacact gccatcactg aataacagat gggcaaaatc ataatcgaca aagctttcga
     8821 tatggtcagc aatactattg ctgatgggcg tatcttcttt tcgcaattca tcaatcacca
     8881 ataacaaccc tcgttgtttg ctttgagtta cactccgaat agctttacgc aggacaggaa
     8941 acttctcgct atctcggctg gaaatacctg ttaagaacgt gagaatatca atggcaaggc
     9001 tttctgaatc tttcgggagt tttaaaatca cataagggtc aagtaaccct ttattgtcgt
     9061 tctcactcgt gagattaaca atattgattt catgggcaat atcaggtaag gtttctttcc
     9121 attcaccacg ttcagctttt gggtcaacaa tggttgcctg tccgccaaag agtactgaat
     9181 aatagaccag catattgtta gagaatgatt ttccgccacc taatgaacca ataaaagcgg
     9241 acgctaatgc atttgttaca gagcctttaa ttccttgact tgcaaggcta ggttggagat
     9301 aaatatttct gcctgtatcg agattgtaac cgacgtaaat accagaattt tcaccaatca
     9361 tttgtgtagc accgaaacca agaccagcca aaaaatccga cgtgacatat tgaatatagt
     9421 cattgatata tcgcttgctt gagggaataa attcattatg caagcctagc atatcgccaa
     9481 aaggtcgtac taatttgata gacagatcat cataaaaatc ttttacttca tcacaacgtt
     9541 gtttcagctc gtccaaattc tttgctgata cccgtaccac atagcttagt ttgtacatag
     9601 attctttggt ttggtctaaa ttactttcta attcatcgac agattctaaa gcgtccacca
     9661 cattagtact ggtttcgttg tcgctttccc atgcgtggct atctaaatct ttcagttctt
     9721 tctttttatt gcgaacggta gataaggctt ttttattggc gacaatttca acattcatac
     9781 tggtatcaac ggggaaagta aactgctgtt gctggtaata aaaaatttca ctattaggga
     9841 aatccaattc accaacaata ctattaattg taaaatacgc cacataagta gtggtatctt
     9901 cctgttcaat ccgtaaatag cgttgatttt cttcgattaa acagcgagtg ggtttgagaa
     9961 tatcgtattt ctttaccaac acttttttat cttcgtatcg tttcggtaaa tggtagtcgt
    10021 agtcatcaaa agggacacct gtttgcccgt aaagatgttc aataatataa ccaaaatcat
    10081 ctttctccaa tttacgaatc tgaaagcgtc tggataattt actttctaac aactgttcca
    10141 tcttagaaaa gcgttcgatt tccgccatag gcatagaaac aaaatcgccc attaatttgt
    10201 gattcacacc atgaaagaat tccgcaaatg ctttttgaat ttctttccca acatttttta
    10261 cggatacttc ctgttcattg aggtagagtt taaaaccaat aaaaaagcga tagtctactt
    10321 gattgtcacc aatcatagaa attaacgctt cggtctgttc atctattttt tcgtaagcta
    10381 tctcttttaa tcgtccagtg atttcttttt tcgattgttc ctgtgtggct cgaatacttg
    10441 attcggttgc tagttgcaga gcatgaatct tgccatcacg attttgggca atcaattgac
    10501 agaaattatc atgaacttga attttttcat cggaacttaa aaaactgtaa ttatagggaa
    10561 ttaattcata ataagcgaat acttcgccat catgattaaa tacgagatta tcttccatat
    10621 atttaatcgg atatgccatc tagttgttca ctccttacaa tcgtaatcga ttcattcatt
    10681 gtttcttttt ttaatcgtat ttgtttgcca gcgtaggtta ctttagggcg tagagcatag
    10741 gtaatgatag actttagaaa tccatagggt ttctttccgt caaaggtctt ttggcacatg
    10801 aacccagtga gaacaatggg aatccctaag tatttgagta gtgctccgtc aatcagagaa
    10861 agtggtggta aactgccaaa gagtatgacg acaaacaaag aaacgataaa ccatgtcatt
    10921 tgcgtaaagg taattgggaa agggagttgt aaatcattaa tcgcataaat cactttttct
    10981 actgaccaaa tactggtgta acttcttatt tttttcatgt cttcacgtcc tttccgtaaa
    11041 aatagaaaaa gaacaatcaa aataggtgat tgctctttca atgctttaac tattcaattt
    11101 tattttttgt ccattttgca ataatacctt catcagtttc ttcatcatct ggattcattg
    11161 aatatctgaa acttaaattg gtatctgacc ctaatcgttc atataagctg ggtctataca
    11221 atctcgtttt attgtcatta aaaggacttc gttttcccag ctcaccattt ccctttaaag
    11281 gatttcttct aactgaaaaa caatattttt ttctgggatc ttctgaatca ctttgactta
    11341 atgagaaaca catggcttct ttttggtctt catttttctt ctcactaact tctgtcggaa
    11401 taaatcgagc aagagtttgg ctaaattgaa ataaaacatc tcccaagtta ctactgtatt
    11461 cgataccaaa atattctctt attgttttcg tgtctgtata taattttacc gaattagcat
    11521 agacagctag catatctgaa aaatcgtttt cctttaaaaa ttctagtttt aacagagcat
    11581 attctggaac tttttcctct ttttcaaata gcttaacaag tacaatataa ttttgctgtt
    11641 tgtaacgaaa gtaaaaggaa tctatcaccc agcctt
//
LOCUS       DARBFD010000292        10660 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.guided.089, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000292 DARBFD010000000
VERSION     DARBFD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.guided.089"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(204..2162)
                     /locus_tag="SCK08_003020"
     CDS             complement(204..2162)
                     /locus_tag="SCK08_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1202813.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="SCK08_003021"
     CDS             complement(2159..3031)
                     /locus_tag="SCK08_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1202814.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="SCK08_003022"
     CDS             complement(3373..4245)
                     /locus_tag="SCK08_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1202815.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="SCK08_003023"
     CDS             4373..4942
                     /locus_tag="SCK08_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG1202816.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="SCK08_003024"
     CDS             5009..5746
                     /locus_tag="SCK08_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1202817.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="SCK08_003025"
     CDS             5977..7245
                     /locus_tag="SCK08_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1202818.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="SCK08_003026"
     CDS             7732..8196
                     /locus_tag="SCK08_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1202819.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="SCK08_003027"
     CDS             8180..8809
                     /locus_tag="SCK08_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1202820.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="SCK08_003028"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="SCK08_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1202821.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT         3441 a         1992 c         2143 g         3084 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc
//
LOCUS       DARBFD010000293         8061 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.guided.110, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000293 DARBFD010000000
VERSION     DARBFD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.guided.110"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            128..1564
                     /locus_tag="SCK08_003029"
     CDS             128..1564
                     /locus_tag="SCK08_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1202822.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="SCK08_003030"
     CDS             1753..4200
                     /locus_tag="SCK08_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG1202823.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="SCK08_003031"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="SCK08_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG1202824.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="SCK08_003032"
     CDS             complement(5690..6793)
                     /locus_tag="SCK08_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1202825.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="SCK08_003033"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="SCK08_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1202826.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2696 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DARBFD010000294         5858 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.guided.129, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000294 DARBFD010000000
VERSION     DARBFD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.guided.129"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            230..1126
                     /locus_tag="SCK08_003034"
     CDS             230..1126
                     /locus_tag="SCK08_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1202827.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1276..2217)
                     /locus_tag="SCK08_003035"
     CDS             complement(1276..2217)
                     /locus_tag="SCK08_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1202828.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2343..3464
                     /locus_tag="SCK08_003036"
     CDS             2343..3464
                     /locus_tag="SCK08_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1202829.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3722..5224
                     /gene="eat(A)"
                     /locus_tag="SCK08_003037"
     CDS             3722..5224
                     /gene="eat(A)"
                     /locus_tag="SCK08_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1202830.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5252..5818
                     /locus_tag="SCK08_003038"
     CDS             5252..5818
                     /locus_tag="SCK08_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1202831.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1936 a         1031 c         1223 g         1668 t
ORIGIN      
        1 attaagaata aatagcaata tttaacaatg actaatgata acaaattttc agacaattac
       61 aactattcag aaaactttta ggcgaatttt aatagaagaa tgatttattt catcatattt
      121 tcatacaaat gttaaatttt catacaaata taggaaacgt tttactggct ttttactatt
      181 taaacaggct atcataggaa aaagtgacag agaaagtgag gcggaaaaga tgaacaaaaa
      241 tgacgtactg ctagttgcaa aaaataaatc actttatttt ttgccttcca tccgctcttt
      301 agatgtttgg tccggaaaag atttactaca gtcggaattc attaaatcag ctagacaaca
      361 gttggcaaat tatcagctga tcgttttttt agattatgga tttgatatgg agatggctcg
      421 acgcgtgcgc ccatttacca atgcaaaaat tgtgttattt ttttggaatc acttcaaagc
      481 agaacacgag gcgatgctga agcaggcaca agatgaaccg gcaatcgatg agatttatca
      541 ttttgatttt ttggaagcaa aagaacttgg gctgaaacat aatagttcat tttattcgaa
      601 acatatgaat ttttcttcta gcgaaccaaa gacggatcta ttttttggtg caacgaataa
      661 tgggagaaaa gaacgagcag aaagctataa gcaggaattt ataaaaagag atgttacgac
      721 caactatttc attcttccac atcgagggaa tgaacaacct ggatatctta cttatgaaga
      781 gtacctgaaa aaaacagggg aatcgcgcgg gattctggaa ctgttgcggg agggtcagca
      841 aggagtgact ttgcggacat ttgaatccat gtattttcaa aagaaactag tgacggataa
      901 tcaagcgatt gctcattatc atttttatcg tcctgaaaat atttttttgc ttcgagaacg
      961 tgatttagat gaattaccgg aatttctgca tacaccattt aagccaatcc tatcagagct
     1021 attggaattt tatgatgcag aaaattgggc aaaacgattt atcactaacg attccactat
     1081 ttatgagcaa tacgaatatg acctctcctc tgttgaaaac aaataaaaaa cctacatttt
     1141 gaagagatta tgtactgaaa caaacaagca caattcttat ggcatcatga gagttgtgtt
     1201 ttctttttga tcttttattg ggcttaaaaa aagagaacca gctgctgatt cagctgattc
     1261 tcatcagaga gccttttatt ctgcgccaag gattccttct tttgcatgga ctaattgggt
     1321 caaaaaggca caatatggtg tagcaatatc atatttttgt ccttttcgcc aaatggcacc
     1381 attgatgtag tcgatttcag tcaaacgatg gtttttgatc aggtcttggt acatagatgg
     1441 gtaatgcaac ccgatgttgt caggatcata agtagaagca atatgttggt acacttcttc
     1501 ttgatctagt atgattcctt cttttgctgc gatagaagca aattcagata cgattgttcg
     1561 aaccatgctc tctgccgctt tttgcgcgcc tagttcagca atattacaat ccaaaatcgt
     1621 gcataaacca ttcaatgttc catttacaca agcttttcgc caaatggaat aacgaacgtt
     1681 atggctatag atcggattta aatttgcttc ttgaaagact tctacaactt tttttgtaaa
     1741 tgcttcgcct tctggttcca agttctctag ttcgatttct ccatcaccta acaatgtgac
     1801 ttttccggga ccagctagac cagctgtcca catcgtgatc ccaaataaga tatttttctt
     1861 tggcacatat ttttccagta catcttcatg acctaatcca tttagtaaac acaaaacata
     1921 agtattgtca gtaataatgg attggatcga acaaaacata tcatctaatt gattggcttt
     1981 tgttaatgct acgatcaagt ccacgtgttc gttgctttcg atgatttctt ctggtgaata
     2041 gattggcagt tttgcgacca cttctttacc gttaaagtca gcaatcaacc catttgtacg
     2101 aatggcttca atgtgagcag gccaacgatc gatcaagagg acctcatttc cgctttgatg
     2161 tagcatcaat ccgattcggc tgcccattgc tccggcccca gctattgcga ttttcatgtt
     2221 ttttctctcc tttattcaat gagttccctt ttagtatata gaaaaatgga agaaaatagc
     2281 attgttttgt ttgaaaaaaa tgaaaaaaag tgtcttactt aagaagaaag tgagggatac
     2341 agatggatat tttggcggca attgattcat ttaaaggaag tgcttcttca tttgaactaa
     2401 atcaggcagt tctaaatggc tgggaagatc gtaaaggaag aaaaatcaat gtgccgattg
     2461 cagatggcgg agaaaatact gcagaagcaa tctattacgc gctcagggga acatggcgta
     2521 ccattgatgg gatggatctc ttattccgtc agcgaaaaat cagctattta ttgacaactt
     2581 ggcaaggaaa aaacaccgca gtcatcgaat ctgcacaagc attggggtta gatctacttg
     2641 ataaaccaac ggatcaaact atccgacttg cttccagtta tgccttagga gaacttatta
     2701 aagatgcact gcttcaagaa gtagaacaaa ttattgtcac tttaggagga agcgggtgta
     2761 gcgatggtgg tctaggatta ctccaatcct tgggtgcttc tttagaaggg tacacagaag
     2821 gaaatccttt attatctact aaaaaacttc attttgatcg aagaaacgtt cttgtttcta
     2881 aagtaaaatt gattatcgct gcagatgtta ccaatccata tacaggtcaa caaggagctg
     2941 cttggcaatt tggaagacaa aaaggaggaa tagatacgac gctagctttc ttagataaac
     3001 aagcaggaaa gattgctcag caaataaaag atttctggga ggtggattta gatgatattc
     3061 caggaagtgg agcagcaggc ggtattggtg gagccctttt tttgttggga gcgaagatgg
     3121 tttctggttt cgatttagtc agccaaatca ctggattgga aaaaaagatc gaacaagcag
     3181 atgtcatcat tacaggagaa gggcggatcg atcagcaaac gatccacgga aaagttccat
     3241 acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat aatcgctatt tgcggaagta
     3301 gagaccaaga gctagaagga ctttctgatt ttctcccaat tgtcttaagt attcatttgg
     3361 gtccaatagg tttagaagaa gcaatggaac acaggcaaac attagaaaaa acaaaaatct
     3421 taggacagac acttagcaga tttttcacca tcctcccgaa gtagttgaca aaatcatttt
     3481 ctttatgcta tcgtaatttc aaatatagga caaagtgagg gatccgtgca ttgaaaaatt
     3541 aagtcgattg atgaaagctg cattcagact gtaccttgtg gagacaagga agcagtgtgc
     3601 agattttcgg gattggcttg gttttttcga tgtatgggtc tttttgtgtg tatccaaccc
     3661 ttgcatcctc tttagccagt tctgaaaata ggactggtct ttttttacga ggtgatggaa
     3721 aatgtctaaa atcgaaataa aaaatctgac attcggctac gacagccaag gcacattatt
     3781 atttgaacaa gcaaatctaa attttgacac acaatggaaa ctaggactta tcggacgaaa
     3841 cggtcgagga aagacaactt tactgaatat tctacaaaac aaactacctt atcaagggca
     3901 agtaatccat cagcaagaat ttgcctattt cccgcaacag acaaaagata aagaacgttt
     3961 aacctattac gtgttaaatg atattacgga ttttgagata tgggaaatcg aaagagagct
     4021 ccaattgatg caaacagatc ctgaaatctt atggagagaa ttcagcacac tatcgggggg
     4081 agagaagaca aaagtcctac tggcactttt atttgtggat gacactcatt tcccgttaat
     4141 cgatgaacca acgaatcatt tggatatctc tggtagaaaa caagtagcgg cttatttgaa
     4201 aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg ggatttatcg atgaagtagt
     4261 ggaccatgtt ttagcaatcg aaaaaagtca actggaactt tatcaaggga atttctctat
     4321 ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg gctcaaaatg aaaaattgaa
     4381 aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa gccgaatggt ctcgttcccg
     4441 agaaggagat aaaacaaaga aacaagtcgg attcatcgat actgaatcta gacgagtgaa
     4501 taaaggagca gtgggtgctg atgctgcacg gacgatgaaa cgatccaaag caatcgtgaa
     4561 tcggatggag acccagatca gcgagaaaga aaaactatta aaagatatcg aatatatcga
     4621 ttctttgacg atgaatagcc aagcgtctca ccataagcga cttttaagcg tagaagatct
     4681 tcaattaggg tatgaaaatc tgttattcga gccaattcat tttacaatcg agcctcatca
     4741 gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca tccattatcc attatcttct
     4801 gggggcattc aacggcaagg ttataggaga aaaataccag ccaaaacatc tgagcattag
     4861 ttatgcaagc caaaattatg aagacaatcg aggaacgttg gcggaatttg cagagaaaaa
     4921 ccaagtagac taccaagcat ttttgaacaa cctccgaaag cttgggatgg aaagagatgt
     4981 ttttcataac aagatcgagc agatgagtat gggccaacgg aaaaaagtgg aattggctaa
     5041 atctttatca cagccagctg aactatatac atgggatgaa ccattgaatt atttggatgt
     5101 cttcaatcaa gaacaattag aacaactgat cttgaacgtg aaacctgcca tgttactagt
     5161 ggaacatgat caaaccttcc tggataaagt atctactgag attatttctc ttgagagaat
     5221 ctaacccttg taatctcaag aacaacaagg tatggttaag gggaactttg ccaacgaagg
     5281 agagaatcac atgctttatg taacgagaca cggagaaaca acttggaacg cacaaggatt
     5341 agtttgcgga cgggcagatg tgccgctgac tgaaaaagga cagatgcagg cacagaaact
     5401 agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa atcatccatt cccctcttca
     5461 acgagcaaga gatacagcac aagcagttgc tgatcgtctc agcttgccgc tgaccgttga
     5521 tgaacgattg gtagaaatgg attttgggga ttatgatgga ctgccaagca aagatgagaa
     5581 ctttcaaaaa gcacgtttag cgttcgctgt acgattccca aatggggagt cagtcttaga
     5641 tgtgtatgca cggatcgttc ctttattgaa agaatgcatc gaagacgaag aaaatgtcta
     5701 tctgttagtt tgccataatg ccttgataag agtgatcaat gcttattttc atccgatgcc
     5761 taatgagggg ttctttacat tcatggtaga taatacagaa ttggtttctt atgagtaatt
     5821 atgtttttgt gttatttaaa taaaatattt caaggcac
//
LOCUS       DARBFD010000295         5613 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.guided.135, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000295 DARBFD010000000
VERSION     DARBFD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.guided.135"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(40..1026)
                     /locus_tag="SCK08_003039"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="SCK08_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="SCK08_003040"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="SCK08_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1202832.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="SCK08_003041"
     CDS             1851..3005
                     /locus_tag="SCK08_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1202833.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="SCK08_003042"
     CDS             3220..4188
                     /locus_tag="SCK08_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1202834.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="SCK08_003043"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="SCK08_003043"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1202835.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="SCK08_003044"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="SCK08_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DARBFD010000296         4061 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.guided.157, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000296 DARBFD010000000
VERSION     DARBFD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.guided.157"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..596
                     /locus_tag="SCK08_003045"
     CDS             <1..596
                     /locus_tag="SCK08_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1202836.1"
                     /translation="LKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMIN
                     NGMPQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEV
                     DDVELQWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLE
                     RTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG"
     gene            603..980
                     /locus_tag="SCK08_003046"
     CDS             603..980
                     /locus_tag="SCK08_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1202837.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1134..1916
                     /locus_tag="SCK08_003047"
     CDS             1134..1916
                     /locus_tag="SCK08_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG1202838.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2042..2773)
                     /gene="erm(A)"
                     /locus_tag="SCK08_003048"
     CDS             complement(2042..2773)
                     /gene="erm(A)"
                     /locus_tag="SCK08_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG1202839.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2831..2890)
                     /locus_tag="SCK08_003049"
     CDS             complement(2831..2890)
                     /locus_tag="SCK08_003049"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG1202840.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            3282..3944
                     /locus_tag="SCK08_003050"
     CDS             3282..3944
                     /locus_tag="SCK08_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1202841.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         1415 a          589 c          768 g         1289 t
ORIGIN      
        1 cattaaaaca agaaacattt agaggtgagt taaataaatt agcttatgag caaaatatag
       61 tggataaatc aggtgagatt tatagattcc atgcccatgc ctttcgccat acagtaggaa
      121 caagaatgat taacaacggg atgccccagc atattgtgca gaaatttttg gggcatgaaa
      181 gcccagaaat gacaagcaga tacgctcata tctttgatga aactctaaaa aatgaattta
      241 ctaaatttca ggaaaaactg gttaccaata atggagatgt gcttgatcta gatgaagata
      301 atgaagtcga tgatgtagag cttcaatggt tcaagaaaaa tataaatgca caagtgcttc
      361 caaatggtta ttgtagattg ccagtagtag caggtggttg tccacatgcg aatgcatgct
      421 tagattgcac tcacttctgt accagtaagc aattcttacc acagcacgaa gaacagttag
      481 agcgtacaga agagttatta gccatagcta aggataaaca atggcaaaga caagtagaga
      541 ctaatagccg tgttaaagag cgtttagaac aaatcattgg aagtttgacg gggtaattat
      601 caatggataa acaagttaga aatacaacag aaattgtacg tttggcgaag cagaaatcaa
      661 aaaagacaag ggaaaaagta gacaaagcga tttctaaatt ttcgattgaa ggtaaagtta
      721 ttaattttaa ttcaatagca aaggaagcta atgtttctaa atcatggctt tataaggaac
      781 acgatattag gcaaagaatc gaatcccttc gtgagcgtca aataacagca aatgtagtct
      841 caaaacccaa gaaaagttct cgttcggagg aaatccttat taaaacctta aaaagaagag
      901 taatggaatt agaaaaagaa aataaaaaat tacagaacca aattcaaaaa ttatatggag
      961 atctgtataa taaagaataa ttattaatct gtagacaaat tgtgaaagga tgtacttaaa
     1021 cgctaacggt cagctttatt gaacagtcat ttaggtatat gtccaatcta gggtaagtaa
     1081 attgagtatc aatataaact ttatatgaac ataatcagtt acgaggtgaa atcatgagca
     1141 atttgattaa cggaaaaata ccaaatcaag cgattcaaac attaaaaatc gtaaaagatt
     1201 tatttggaag ttcaatagtt ggagtatatc tatttggttc agcagtaaat ggtggtttac
     1261 gcattaacag cgatgtagat gttctagtcg tcgtgaatca tagtttacct caattaactc
     1321 gaaaaaaact aacagaaaga ctaatgacta tatcaggaaa gattggaaat acggattctg
     1381 ttagaccact tgaagttacg gttataaata ggagtgaagt tgtcccttgg caatatcctc
     1441 caaaaagaga atttatatac ggtgagtggc tcaggggtga atttgagaat ggacaaattc
     1501 aggaaccaag ctatgatcct gatttggcta ttgttttagc acaagcaaga aagaatagta
     1561 tttctctatt tggtcctgat tcttcaagta tacttgtctc cgtacctttg acagatattc
     1621 gaagagcaat taaggattct ttgccagaac taattgaggg gataaaaggt gatgagcgta
     1681 atgtaatttt aaccctagct cgaatgtggc aaacagtgac tactggtgaa attacctcga
     1741 aagatgtcgc tgcagaatgg gctatacctc ttttacctaa agagcatgta actttactgg
     1801 atatagctag aaaaggctat cggggagagt gtgatgataa gtgggaagga ctatattcaa
     1861 aggtgaaagc actcgttaag tatatgaaaa attctataga aacttctctc aattaggcta
     1921 attttattgc aataacaggt gcttactttt aaaactactg atttattgat aaatattgaa
     1981 caatttttgg gaagaataaa gcgtcctctt gtgaaattag agaacgcttt attactttaa
     2041 tttagtgaaa caatttgtaa ctattgaaaa tagaaagaaa ttgttccttc gatagtttat
     2101 taatattagt gacatttgca tgcttcaaag cctgtcggaa ttggttttta gtgaaaagaa
     2161 cacgatattc acggtttacc cacttataaa caaaagatcg atactttttg tagtccttct
     2221 ttgaaatcaa tggttgatgt cgttcaagaa caatcaatac agagtctaca cttggcttag
     2281 gatgaaaata tagtggtggt acttttttga gcatttttat atccatctcc accattaata
     2341 gtaaacccaa agctcgttgc agattttgca atcttttcgc aaatcccttc tcaacgataa
     2401 gatagctata tttagcctga ctttcaaagg taattctttt gacaatatcc gtactgatgt
     2461 tataaggaat attaccatat atcttatagt ttatatgttt tgggaaggaa aattttagaa
     2521 tatccgtttg aatcactttt atattctcag aggggtttac cgcttcttta gtcacttgac
     2581 ataagcctcc atcaatttct atagcagtaa ctgatcgact cattttgact agctctttgg
     2641 taaaatgtcc ttttcctgat ccgatttcta ttacgttgtc ttgtttactg atattcgtgt
     2701 gattcaatat ttcttttaca tgctttttag aagtaataaa attttgcgtg tctttagggt
     2761 ttttctggtt cattataacc ttctccttac tggttataat gaactgacat taagcgttca
     2821 ttataaccaa ttaattttga tttggttgat aacgaacttt attaataaca aatatagaaa
     2881 aagtacccat acttttatct cctttccatt tttttcatta tgaatgagat aaagtaattt
     2941 ctactactgc gatactggtg cacataattg aaacctcctt atgtaactga atatgattgt
     3001 atcatctttt cattttagaa taaaccttta tatgtaattt tgcacttgac acttcagatg
     3061 gacgttttct tcaaaataat atgttttgaa acttgataag agaagccact gtctttacta
     3121 aaaagtaaag gctgcgataa aggacagtga ctttaataaa gttgcactgt tgtattgtct
     3181 ttggataaaa ttgtctaaag ggaagtttta tccccaacga aaaaacatct aaagtggtaa
     3241 gtaatgaaag aatgtcgata aagtaggaat gggggatctt tttgaataca atagaatcac
     3301 agtttgataa ggttgcagaa gattacgatt tcgtgaatga gcttttgaat gattattcat
     3361 tctttgtgtc taatatgtct ccaaagaaag gcagagcatt agatatcgga tgtggctcgg
     3421 gtttgttagt ggagaaatta gcaagttatt atgatgaagt ggtagggatt gatatttcta
     3481 atcaaatgct cgatcttgcc aaatctaaac gtcaactaac aaatacggtc tatctgaata
     3541 tgaatgcaga acaacttaat tttaatgaga agtttgattt tattgtaagc cgaacaacct
     3601 ttcatcattt ggatgatata gccagcgtaa tacagcaaat gaaggaactg ttgaatgagg
     3661 aaggaagaat agttattctt gataatgtat ctgaagtcga gacaccgcct acctatgttt
     3721 ataaattagg ggcaatccaa gagtttttac cgcattgttt caaatttgga ataaaaaatg
     3781 cgattagaat ttataatcac aacacatcta aatcatggct tgagcattta gcttcagata
     3841 agtacctatc tgaacaaaat tattacgatt tatatgaaaa gttgttgcct ggatgccaat
     3901 tccataaaat gggttgggca atgggggttg tctggacaaa ataacttaaa gctgtggctt
     3961 tgaaaagttg aatttgtgat tgttttcaga gctatcgggt gctttagtat aaataacaat
     4021 gaataaaaaa tcagcctgta tcttatgtta ctattaagta a
//
LOCUS       DARBFD010000297         2354 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.guided.190, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000297 DARBFD010000000
VERSION     DARBFD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.guided.190"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            344..1384
                     /locus_tag="SCK08_003051"
     CDS             344..1384
                     /locus_tag="SCK08_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1202842.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1482..1670
                     /locus_tag="SCK08_003052"
     CDS             1482..1670
                     /locus_tag="SCK08_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG1202843.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1728..2081)
                     /locus_tag="SCK08_003053"
     CDS             complement(1728..2081)
                     /locus_tag="SCK08_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1202844.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT          783 a          375 c          400 g          796 t
ORIGIN      
        1 aactaattta ccatctaatt tcttatttct atcaaaaaac cttttctcat tatcctgccc
       61 gttagttgaa taagcaattt tttctataag ttatattatc ataataccac aaaatcacaa
      121 attttcattc aattatcatt ttcaattgca ttttataaat cattttgtat gtaatttggt
      181 atgtaagtta aaatgaaaaa agtagtccat tttgaagtac attttgtcat tttgtactcc
      241 aaaactgaac tacttacttg catatatacg cctttatagt ggagtactac atttacgaga
      301 ttcggttaga gtatcagaaa aagaaaaaat aaaagttaca gaaatgtata cttcaataaa
      361 tggtgaatta tgtaagattg atagagctta ttctggagaa attgttattt tgcaaaatga
      421 gtttttgaag ttaaatagtg ttcttggaga tacaaaacta ttgccacaga gaaaaaagat
      481 tgaaaatccg caccctctac tacaaacaac tgttgaaccg agtaaacctg aacagagaga
      541 aatgttgctt gatgcccttt tggaaatctc agatagtgat ccgcttctac gatattacgt
      601 ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa tggaagtgat
      661 tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc ctacagtcat
      721 ttatatggag agaccgttaa aaaatgcaga atataccatt cacatcgaag tgccgccaaa
      781 tcctttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa gtggaatgca
      841 gtatgagagc tcggtttctc ttggatactt aaatcaatca tttcaaaatg cagttatgga
      901 agggatacgc tatggttgcg aacaaggatt atatggttgg aatgtgacgg attgtaaaat
      961 ctgttttaag tacggtttat actatagccc tgttagtact ccagcagatt ttcggatgct
     1021 tactcctatt gtactggagc aagcctttag aaaagctgga acagaattgt tagagccata
     1081 tcttagtttt aaagtttatg caccacagga atatctttca cgggcatata acgatgctcc
     1141 caaatattgt gcaaatatcg taaatactca actgaaaaat aatgaggtca ttattattgg
     1201 agaaattcct gctcgatgta ttcaagatta tcgcaatgat ttaacttttt ttacaaatgg
     1261 gcttagtgtt tgtttagcag agctaaaagg atatcaggtt accactggcg aacctgtttg
     1321 ccagacccgt cgtctaaata gtcggataga taaagtaaga tatatgttca ataaaataac
     1381 ttagtgcgtt ttatgttgtt atataaatat ggtttcttat taaataagat gaaatattct
     1441 ttaatataga tttgaattaa agtggaaagg aggagattgt tattataaac tacaagtgga
     1501 tattgtgtcc tatttgtgga aataaaacaa gactacgaat acgagtggat actatactta
     1561 aaaatttccc tttatactgc cccaaatgta agaacgaaac tttaattaat gttcaaaaaa
     1621 tgaatataat aacaatcaaa gagccagacg ccaagacgca gagccgataa tttgagaaat
     1681 gaaactctca tcttatcggc tctttttgtt tatctgaatt ttactgacta gccttcaata
     1741 tttcttgctt cattgatacc ttttgctagt gcttccatta aggatagttc tttgtctgta
     1801 aagctatcca tgtatttttc tacctgtaac cttcgagtgc ttttaatcaa attatctgta
     1861 ggtaagaaaa attcatcaat agatacatgg agtaatgata caagatcata cagcacttgt
     1921 gtacttgggt gttgtccttt gttttctata tttgttaaat aacgtgggtc aatttcaatc
     1981 attgttccca cttgttcacg agtgagaccc cgtttcattc gagcctcttt aatcgctaaa
     2041 ccaacggctc taaaatcgta tttatcttct tttttacgca tactaaatca cctctataca
     2101 ttttactgtt cctatataac cataaacagg tatagaaaaa catgttatat agtttatgag
     2161 ttcctataga acactgtgga atttattaat gcgatgaatc caatttcaaa tagaaaggtc
     2221 gctgatttta tttacctata caactttata aaatttagtc atactcctag aaagtagata
     2281 aaattggcga cttaatggag ggaaaagtcg ccatgagaaa gataatctgt catctagttc
     2341 tcttaattaa gttc
//
LOCUS       DARBFD010000298         2126 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.guided.193, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000298 DARBFD010000000
VERSION     DARBFD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.guided.193"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(757..1047)
                     /locus_tag="SCK08_003054"
     CDS             complement(757..1047)
                     /locus_tag="SCK08_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1202845.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1255..1947)
                     /locus_tag="SCK08_003055"
     CDS             complement(1255..1947)
                     /locus_tag="SCK08_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1202846.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          589 a          395 c          313 g          829 t
ORIGIN      
        1 tcagttcttt gttattcgtt atttttctat tatgtttgtt catttttata ctccttctta
       61 tatttcctaa tagagtataa catagcttta agcattactt aaagataact taaaagttat
      121 cttttaaata cttaattgtt aaatacctta tttgatgcaa aagaaatcta cgatagtcaa
      181 caaaaaaatt ttcattatat ccagaaaaat ttttcttagt taaggaccta tggattgcag
      241 taatgcagaa ttcgtcgaat aaactgccaa agacatacaa tcatattgct tttaaaattg
      301 acgaacaaga tatagattcg tttgtctcaa aaatccaaat gttaggttta actgtcgaac
      361 caggacgttc tcgtgtgaaa ggcgaggctt cttcaattta cttttacgac tatgataatc
      421 acctcttcga gttacatacc ggtactctcc aggaacgttt aaaatcctac aattccacta
      481 cctgagacta gtaatgacta gtctcttttt atggcctctg atccccccat tgagcgaata
      541 ctaatagggc ttcgaattga tctgttcaat ctttaagcaa tatccctcaa atgagtttgg
      601 aaagtttagt gattcatcac aaatacttaa atacatgtag ttatatatag attgaggttc
      661 ttgtgagcgt atgctcgtgt gggttcaaat cccatcgtct gcatccatct ttccaaaaca
      721 agcggctagg acatttttgt ctcagccgct tgttttctag tttattcttt catcttgtcg
      781 gacctttttt cttgggtata gacttttctc atattctacc acaattttgg gtttccctaa
      841 cacatgatag gatctcacaa caaattgctt tctattatta taataacctg taacgactaa
      901 cttcatccct tcgtctactt cagctaggaa gttcaaacta tgggaagcaa tcaaacaatt
      961 gaccccattt aaactgaaac ggatcaacgg tctggcacta acttgcaaag tacggatctt
     1021 atctactgtt ccatattttt tctccataac cttcacctca ataccattat acgaacatac
     1081 attccctttg taaagaagaa actctgtttt atctttttga ctttttgtct attacctttc
     1141 ttaaaatcat attggcactt cctgcgaaga tgaaggacat gtactaaaaa aaagaggcag
     1201 cagtatagat tactgttgct tctttactaa tttaggttta cttgttaaga aagattattt
     1261 acgcaaatgt tcttcaacaa tttgctgaga tttatcagct gtatcttcca cttcatcttt
     1321 tgctttttct aacttttgaa tatattgttt caagcttgag ttttcagcag ttgcttcgtc
     1381 taatttcttt tgtgtatctg ttaactgagt ttctacattt gttttttctt gtgtcagtgt
     1441 tgcgatttgt tgattcaaac catttacagt attttgtaat tctgaaagtt ttgcttgtgc
     1501 ttcttgtagt cttgctgtcc aatctgcatt cagatgattg atttcatcat ttttttggtt
     1561 gatctcatct agtttgacat tgatttcatt ttgtttgctt tgtagttgtg catccttgct
     1621 gactaattga ctttctaagt tttgtttttc tgctgccgat tgattttttt ggttttctaa
     1681 ttcatttact tttgtttggt attcttggac atctgccttt gaatcttgca atgtctgttc
     1741 tttttgtgct agtcgagcgg taagatctgt aagcgcttgc tctttatctg tcaatgtatg
     1801 atcaagctgt gtcagattct cattgattgt atcaatatct gtctctccac cccacaaaat
     1861 aatttcattt gcgtaccgcg tgcctgtgta gccaattaga catagaaatg caacgacaat
     1921 cccaccagta ataaatttct ttttcatttg catagctcct taatccaata cttttacatg
     1981 ctaaaagtaa gtgatctcaa acacaatgtc aagcgttttt aaattacatg tgtaatttta
     2041 atataaatac tatcaaatac tcatttaatc ctcctttttt atctatttaa aaaatctttt
     2101 ttatagagaa aaatgcaggt taatac
//
LOCUS       DARBFD010000299         1781 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.guided.204, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000299 DARBFD010000000
VERSION     DARBFD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.guided.204"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            24..416
                     /locus_tag="SCK08_003056"
     CDS             24..416
                     /locus_tag="SCK08_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1202847.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="SCK08_003057"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="SCK08_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HEG1202848.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DARBFD010000300         1504 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.guided.215, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000300 DARBFD010000000
VERSION     DARBFD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.guided.215"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            262..345
                     /locus_tag="SCK08_003058"
                     /pseudo
     CDS             262..345
                     /locus_tag="SCK08_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="SCK08_003059"
     CDS             394..477
                     /locus_tag="SCK08_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG1202849.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="SCK08_003060"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="SCK08_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG1202850.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DARBFD010000301         1382 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.guided.222, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000301 DARBFD010000000
VERSION     DARBFD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.guided.222"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1311
                     /locus_tag="SCK08_003061"
     CDS             <1..1311
                     /locus_tag="SCK08_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HEG1202851.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DARBFD010000302         1350 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.guided.227, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000302 DARBFD010000000
VERSION     DARBFD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.guided.227"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            47..298
                     /locus_tag="SCK08_003062"
                     /pseudo
     CDS             47..298
                     /locus_tag="SCK08_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="SCK08_003063"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="SCK08_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG1202852.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DARBFD010000303         1194 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3939
            SAMEA114569800-rid20112093.guided.236, whole genome shotgun
            sequence.
ACCESSION   DARBFD010000303 DARBFD010000000
VERSION     DARBFD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569800
            Sequence Read Archive: ERR12203625
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972608.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972608.1
            Genome Coverage       :: 150x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:19:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,064
            CDSs (total)                      :: 3,003
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569800-rid20112093.guided.236"
                     /isolate="VRE3939"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="SCK08_003064"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="SCK08_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEG1202853.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
